CN113016600B - Method for degrading sulfonamide antibiotics in decomposed livestock and poultry manure - Google Patents
Method for degrading sulfonamide antibiotics in decomposed livestock and poultry manure Download PDFInfo
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
A method for degrading sulfonamide antibiotics in decomposed livestock manure by utilizing plant root system secretion comprises the following steps: a hydroponic facility was built and designed as 4 layers altogether. Water planting nutrient solution: decomposed manure leachate=10:1, and the leachate is sterilized and diluted. And (3) selecting hydroponic plants, namely respectively selecting low-temperature plants and high-temperature plants. The plant spacing of the hydroponic plants is 15-25cm. The hydroponic plant is changed for one time in 6 days, and is shaded by a shading net at high temperature. The antibiotic degradation platform is designed into 4 layers, each layer is a tray with the depth of 15-20cm, the area of the tray is 60cm multiplied by 35cm, and the tray with the bottom layer of 70cm multiplied by 35cm is used for receiving seepage. And the same side of the tray is lifted by 1cm, and the other side of the tray is reserved with holes by using cloth strips for drainage. And designing a water pipe with holes for supplying water. The plant water culture solution is added once every 6 days when the plant water culture solution is decomposed, and the total degradation time is 30 days. The degradation rate is measured, the total content of the sulfonamide antibiotics is 0.5-1mg/kg, and the degradation rate is 60% -80%.
Description
Technical Field
The invention belongs to the field of degradation of organic pollutants, and particularly relates to a method for promoting degradation of sulfonamide antibiotics in decomposed livestock and poultry manure by utilizing plant root secretions.
Background
The sulfonamide antibiotics are artificial synthetic antibacterial drugs with wider antibacterial spectrum, have certain killing power on gram-negative bacteria and gram-positive bacteria, and mainly inhibit the activity of microorganisms. The feed additive has the advantages of stable property, simple and convenient use, no consumption of grains in production and the like, and is widely used in feed additives in livestock and poultry cultivation. The fresh livestock manure contains a large amount of undegraded sulfonamide antibiotics, and the sulfonamide antibiotics can be obviously reduced after short-term storage, but a certain amount of residues are also left. When the organic fertilizer is applied to the farmland, soil antibiotic pollution is caused, the plant absorbs and accumulates sulfonamide antibiotics, and the detected concentration in corns, tomatoes and lettuce is up to 0.1 mg/kg. Sulfonamide antibiotics are absorbed by crops into the food chain and harm human health. The sulfonamide antibiotics are known to be highly toxic drugs based on information released by swedish pharmaceutical industry association. Can cause nausea, allergy and other reactions of human beings, and the accumulation of the drugs in food can cause the development and transmission of drug resistance, and previous researches find that the drug resistance of the sulfonamide antibiotics is highest and is several times higher than that of other antibiotics.
The treatment of sulfonamide antibiotics mainly comprises advanced oxidation technology, adsorption technology, membrane treatment technology, physical and chemical methods and biological methods. The physicochemical method has the advantages of high energy consumption, easy membrane technology pollution, good treatment effect, recyclable adsorbent, high cost of the adsorbent and easy pollution of the adsorbent material in the subsequent treatment. The advanced oxidation method comprises a plurality of methods, and has the advantages of high efficiency, but photooxidation has the defects of complicated catalyst preparation process, high energy consumption, complicated operation and the like. The electrochemical oxidation has the advantages of no consumption of chemical substances, simple operation, but the problems of reduced process efficiency, long service life of the electrode, electrode pollution caused by surface organic matter deposition and the like caused by current density increase. Fenton oxidative degradation of sulfonamide antibiotics has the main problems of catalyst activity and by-product formation in the degradation process. The biological method is a relatively environment-friendly and simple method, and the existing method for degrading the sulfonamide antibiotics by utilizing microorganisms under the anaerobic or aerobic condition is not thorough in degradation.
Disclosure of Invention
The invention aims to provide a method for degrading sulfonamide antibiotics in decomposed livestock and poultry manure, which utilizes plant root secretions to activate microorganisms in the decomposed livestock and poultry manure, degrades the sulfonamide antibiotics which are not degraded in the decomposition process, improves the biodegradation rate, purifies the pollution of antibiotics and increases the fertilizer efficiency of organic fertilizers.
The invention is realized by the following technical scheme:
1. firstly, a water planting facility is established, a three-dimensional water planting device is designed into 4 layers, the depth of each layer of water planting tray is 15-20cm, a sunshade net is utilized for covering (not covering plants), firstly, the growth of green algae is avoided, secondly, water planting seedlings are fixed, the area of an upper layer tray is 60cm multiplied by 25cm, the lower layer tray is 65cm multiplied by 30cm, 70cm multiplied by 35cm, 75cm multiplied by 40cm, the lowest layer is a culture tray with the depth of 20cm and 85cm multiplied by 50cm, seedlings are not placed, water is received, and meanwhile, water in a lower water tray is pumped to the upper side by a circulating pump, so that water planting and supplying are ensured;
2. secondly, constructing a sulfonamide antibiotics platform in the decomposed livestock manure, which is also a three-dimensional platform, wherein each layer is a tray with the depth of 15-20cm, the area of the tray is 60cm multiplied by 35cm, and the bottom layer is provided with a tray with the depth of 70cm multiplied by 35cm for receiving seepage without putting manure. And (3) lifting 1cm on the same side of the tray, draining redundant filtrate to the last tray by using cloth strips on the other side of the tray, and collecting seepage. The water pipe is discharged at the upper side of each layer, and water holes are reserved on the water pipe and are one hole per centimeter;
3. the water culture nutrient solution is from decomposed livestock and poultry manure leaching solution, the water is solid=10:1, the leaching solution is sterilized at 100 ℃ for 10 minutes, and then diluted by 50-100 times, and the water culture plant is used as the nutrient solution;
4. selecting water-cultured plants, and selecting low temperature-resistant plants such as Chinese cabbage, spinach, rape, etc. at a temperature of 10-20deg.C; selecting high temperature resistant plants such as crabgrass, paspalum, green bristlegrass, etc. at 18-28deg.C;
5. the plant spacing of the hydroponic plants is 15-25cm, and is determined according to the plant size;
6. the culture solution is changed once in 6 days, the hydroponic plant ensures the circulation of water, and when the temperature exceeds 28 ℃, a sunshade net is used for shading;
7. water management of decomposed livestock manure: the water culture liquid which has been cultured for 6 days is added every 6 days, and the added water culture liquid is based on the liquid just leached from the bottom. Because the bottom tray is inclined by 1cm, no water is accumulated at the bottom of the decomposed livestock manure, no water is added within 6 days, and the pile is not turned over;
8. the period for degrading the decomposed livestock manure is 5 periods for 30 days;
9. the degradation rate is measured by sampling in 30 days, the total content of the sulfonamide antibiotics is 0.5-1mg/kg, and the degradation rate is 60% -80%.
The invention has the advantages that: the plant root system secretion contains a large amount of biochemical active substances, so that the quantity and activity of microorganisms can be increased in the decomposed livestock and poultry manure by spraying, the degradation of sulfonamide antibiotics in the decomposed livestock and poultry manure is promoted, and the pollution of antibiotics is reduced; the water-cultured plants cultured by utilizing the sterilized decomposed livestock and poultry manure leaching solution can be used as feed, so that the ecological cycle of the system is ensured.
Drawings
FIG. 1 is a schematic diagram of a platform for degrading sulfonamide antibiotics in decomposed livestock manure, wherein the parts are marked as follows: 1 is a water funnel; 2 is a tray; 3 is a water pipe; 4 is a water receiving tray; and 5 is a drainage cloth strip.
Description of the embodiments
The invention will be described in further detail below with reference to the embodiments shown in the drawings.
Examples
1. In a small chicken farm in the ancient elm town of Chang-shi county, a water culture rack is welded by utilizing triangular iron, a water culture facility is established, 4 layers are welded together, the height of each layer is 40cm, the depth of an placed tray is 15cm, the depth of a placed water culture nutrient solution is 15cm, then a sunshade net is utilized for covering (not covering plants), the sunshade net is utilized for 3-needle density sunshade net, and a thin wire is utilized for penetrating and pulling, so that a hole of about 0.5-1cm is exposed for transplanting seedlings. The bottom iron frame is large, the upper iron frame support is welded to the inner side of the lower iron frame, the area of an upper tray is ensured to be 60cm multiplied by 25cm, the lower tray is sequentially 65cm multiplied by 30cm, 70cm multiplied by 35cm, 75cm multiplied by 40cm, the lowest part is a culture tray with the depth of 20cm and 85cm multiplied by 50cm, seedlings are not placed, water is received, and meanwhile, water in the lower tray is pumped to the upper side by a circulating pump, so that water culture and supply are ensured;
2. the method comprises the steps of constructing a sulfonamide antibiotic platform in decomposed livestock and poultry manure, designing a three-dimensional platform, and using triangular iron to put plastic trays with the depth of 15-20cm in each layer, wherein the tray area is 60cm multiplied by 35cm, and the bottom layer is provided with 70cm multiplied by 35cm tray seepage, so that manure is not put. And (3) lifting 1cm on the same side of the tray, draining redundant filtrate to the last tray by using cloth strips on the other side of the tray, and collecting seepage. The water pipe is discharged at the upper side of each layer, and water holes are reserved on the water pipe and are one hole per centimeter;
3. the water culture nutrient solution is from decomposed livestock and poultry manure leaching solution, the water is solid=10:1, the leaching solution is sterilized at 100 ℃ for 10 minutes, and then diluted by 50-100 times, and the water culture plant is used as the nutrient solution;
4. the selection of the hydroponic plant is carried out in 3 months, and the temperature is within the range of 10-20 ℃, so that the low-temperature resistant plant pakchoi is selected; sowing the cabbages in the middle ten days of 2 months, carrying out seedling emergence in a greenhouse for 5 days, and transplanting in 3 months and 5 days, wherein the number of leaves is 4, and the plant height is 8-10cm;
5. the plant spacing of the transplanted hydroponic plants is 15cm;
6. the hydroponic plant is replaced by the culture solution once in 6 days, the temperature is lower, and shading by a shading net is not utilized;
7. water management of decomposed livestock manure: the hydroponic liquid which has been cultivated for 6 days is added every 6 days, the amount of the added hydroponic liquid being based on the liquid just leached from the bottom. Because the bottom tray is inclined by 1cm, no water is accumulated at the bottom of the decomposed livestock manure, no water is added within 6 days, and no pile is turned over;
8. the period for degrading the decomposed livestock manure is 5 periods for 30 days;
9. the degradation rate was measured by sampling for 30 days, and the total content of sulfonamide antibiotics was 0.6. 0.6 mg/kg, and the degradation rate was 75.5%.
Examples
1. In a miniature pig farm in the ancient elm town of Chang-shi county, a water culture rack is welded by utilizing triangular iron, a water culture facility is established, 4 layers are welded together, the height of each layer is 40cm, the depth of an placed tray is 15cm, the depth of a placed water culture nutrient solution is 15cm, then a sunshade net is utilized for covering (not covering plants), the sunshade net is utilized for 3-needle density sunshade net, and a thin wire is utilized for penetrating and pulling, so that a hole of about 0.5-1cm is exposed for transplanting seedlings. The bottom iron frame is large, the upper iron frame support is welded to the inner side of the lower iron frame, the area of an upper tray is ensured to be 60cm multiplied by 25cm, the lower tray is sequentially 65cm multiplied by 30cm, 70cm multiplied by 35cm, 75cm multiplied by 40cm, the lowest part is a culture tray with the depth of 20cm and 85cm multiplied by 50cm, seedlings are not placed, water is received, and meanwhile, water in the lower tray is pumped to the upper side by a circulating pump, so that water culture and supply are ensured;
2. the method comprises the steps of constructing a sulfonamide antibiotic platform in decomposed livestock and poultry manure, designing a three-dimensional platform, and using triangular iron to put plastic trays with the depth of 15-20cm in each layer, wherein the tray area is 60cm multiplied by 35cm, and the bottom layer is provided with 70cm multiplied by 35cm tray seepage, so that manure is not put. And (3) lifting 1cm on the same side of the tray, draining redundant filtrate to the last tray by using cloth strips on the other side of the tray, and collecting seepage. The water pipe is discharged at the upper side of each layer, and water holes are reserved on the water pipe and are one hole per centimeter;
3. the water culture nutrient solution is from decomposed livestock and poultry manure leaching solution, the water is solid=10:1, the leaching solution is sterilized at 100 ℃ for 10 minutes, and then diluted by 50-100 times, and the water culture plant is used as the nutrient solution;
4. the selection of the hydroponic plant is carried out in 7 months, and the air temperature is in the range of 20-30 ℃, so that the crabgrass with the height of Wen Zhiwu is selected; digging crabgrass directly in the field, soaking for 10 minutes by using clear water, and washing roots;
5. the plant spacing of the transplanted hydroponic plants is 20cm;
6. the hydroponic plant is replaced by the culture solution once in 6 days, the temperature is lower, and shading by a shading net is not utilized;
7. water management of decomposed livestock manure: the hydroponic liquid which has been cultivated for 6 days is added every 6 days, the amount of the added hydroponic liquid being based on the liquid just leached from the bottom. Because the bottom tray is inclined by 1cm, no water is accumulated at the bottom of the decomposed livestock manure, no water is added within 6 days, and no pile is turned over;
8. the period for degrading the decomposed livestock manure is 5 periods for 30 days;
9. the degradation rate was measured by sampling for 30 days, and the total content of sulfonamide antibiotics was 0.8. 0.8 mg/kg, and the degradation rate was 77.8%.
Claims (3)
1. A method for degrading sulfonamide antibiotics in decomposed livestock manure by utilizing plant root system secretion, which is characterized by comprising the following steps:
(1) Firstly, a three-dimensional water planting device is established, 4 layers of trays are arranged at the upper part, the depth of each layer of tray is 15-20cm, the areas of the trays are 60cm multiplied by 25cm, 65cm multiplied by 30cm, 70cm multiplied by 35cm and 75cm multiplied by 40cm in sequence from top to bottom, a sunshade net is used for covering, and the sunshade net is pulled through fine wires to expose 0.5-1cm holes for transplanting seedlings, firstly, green algae growth is avoided, and secondly, water planting seedlings are fixed; placing a culture tray with the depth of 20cm and 85cm multiplied by 50cm at the lowest part, not placing seedlings, taking charge of water receiving, and simultaneously pumping water in the culture tray at the lower part to the upper side by using a circulating pump to ensure water culture and supply;
(2) Secondly, constructing a three-dimensional platform for degrading sulfonamide antibiotics in decomposed livestock and poultry manure, firstly arranging 4 layers of trays at the upper part, wherein the depth of each layer of tray is 15-20cm, the area of each tray is 60cm multiplied by 35cm, then arranging 70cm multiplied by 35cm trays at the bottom layer to receive seepage liquid, not placing manure, lifting 1cm at the same side of the trays, and draining the residual filtrate to the bottom layer of tray by using cloth strips at the other side of the trays, collecting seepage liquid, and draining water pipes at the upper side of each layer, wherein the water holes are reserved for one hole per centimeter;
(3) The water culture nutrient solution is from decomposed livestock and poultry manure leaching solution, the water is solid=10:1, the leaching solution is sterilized at 100 ℃ for 10 minutes, and then diluted by 50-100 times, and the water culture plant is used as the nutrient solution;
(4) Selecting hydroponic plants, and selecting low-temperature resistant plant Chinese cabbage under the condition of lower temperature of 10-20 ℃; selecting high-resistance Wen Zhiwu crabgrass under the condition that the air temperature is 18-28 ℃;
(5) The plant spacing of the hydroponic plants is 15-25cm, and is determined according to the plant size;
(6) The hydroponic plant is changed with the culture solution once in 6 days, the hydroponic plant ensures the circulation of water, and when the temperature exceeds 28 ℃, the plant is shaded by using a shading net;
(7) Water management of decomposed livestock manure: the water culture liquid which has been cultured for 6 days is added once every 6 days, and the added water culture liquid is based on the liquid just leached from the bottom; because the bottom tray is inclined by 1cm, no water is accumulated at the bottom of the decomposed livestock manure, no water is added within 6 days, and the pile is not turned over;
(8) The period for degrading the decomposed livestock manure is 5 periods for 30 days;
(9) The degradation rate is measured by sampling in 30 days, the total content of the sulfonamide antibiotics is 0.5-1mg/kg, and the degradation rate is 60% -80%.
2. The method for degrading sulfonamide antibiotics in decomposed livestock manure by utilizing plant root system secretion according to claim 1, wherein the seeding, growth management and transplanting of the low temperature resistant plants are as follows:
(1) Sowing by using a seedling raising tray, wherein the soil temperature is ensured to be higher than 5 ℃; normal water and fertilizer management, proper illumination and ventilation, and free growth prevention;
(2) Seedling transplantation: transplanting when seedlings grow to 5-6 leaves, watering the seedlings two days before transplanting, not watering the seedlings on the same day, tapping a seedling tray by using a wood stick to enable the seedlings and soil to be separated from the tray, shaking out the soil, soaking the seedlings in clear water for 10 minutes to obtain root systems, and taking out the seedlings and putting the root systems into water planting liquid;
(3) Note that root system is not hurt when passing through the sunshade net on the water planting disk.
3. The method for degrading the sulfonamide antibiotics in the decomposed livestock manure by utilizing the plant root system secretion according to claim 1, wherein the construction of the platform for degrading the sulfonamide antibiotics in the decomposed livestock manure is as follows:
(1) The bracket used for constructing the platform is a triangular iron frame;
(2) A plastic tray was placed on the iron stand.
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| CN101811776B (en) * | 2010-04-13 | 2011-12-21 | 南京大学 | Method for processing aquaculture wastewater containing antibiotics by utilizing plant floating bed |
| CN103848502B (en) * | 2012-12-05 | 2016-07-06 | 中国科学院城市环境研究所 | A kind of water plant filter bed system purified containing antibiotic breeding wastewater |
| CN104016484B (en) * | 2013-07-11 | 2016-04-20 | 中国科学院城市环境研究所 | A kind of for removing nitrogen phosphorus and antibiotic upwelling artificial swamp in breeding wastewater |
| CN103332791A (en) * | 2013-07-11 | 2013-10-02 | 中国科学院城市环境研究所 | Artificial wetland capable of strongly removing antibiotic and resistance gene from culture waste water |
| CN105330094B (en) * | 2015-09-06 | 2017-09-29 | 东南大学 | Strengthen the system and method that wetland coupled system removes antibiotic in surface water using root exudates |
| CN107148901A (en) * | 2016-03-03 | 2017-09-12 | 杜晓华 | A kind of implant system being made up of Novel planting rope |
| CN107302884A (en) * | 2016-04-23 | 2017-10-31 | 杜晓华 | A kind of combined type fowl fish and vegetable symbiotic system |
| CN106940361B (en) * | 2017-04-25 | 2019-07-09 | 广东省农业科学院环境园艺研究所 | It is a kind of for go water removal in disulfonamide object plant application and its screening technique |
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2021
- 2021-03-30 CN CN202110337202.5A patent/CN113016600B/en active Active
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