CN112970577A - Breeding method of white jade green cucumber - Google Patents
Breeding method of white jade green cucumber Download PDFInfo
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- CN112970577A CN112970577A CN201911282809.7A CN201911282809A CN112970577A CN 112970577 A CN112970577 A CN 112970577A CN 201911282809 A CN201911282809 A CN 201911282809A CN 112970577 A CN112970577 A CN 112970577A
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- cucumber
- white jade
- jade green
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a breeding method of white jade green cucumber, which comprises the following steps: step S1: selecting male parent and female parent; step S2: carrying out sexual hybridization; step S3: f1 pollen is cultured in vitro to obtain haploid plants; step S4: inducing chromosome doubling to obtain fertile homozygote; step S5: and (3) breeding the white jade green cucumber meeting the conditions by a haploid breeding method. According to the invention, the white jade green cucumber product has the characteristics of excellent quality, crisp taste, small cavity, thick meat and the like, is bright green and white in appearance, has obvious and dense white spines, has straight melon strips and uniform thickness, has smaller leaves than female parents, has increased disease resistance compared with the female parents, is resistant to blight, downy mildew and powdery mildew, is suitable for planting in a cutting frame and is suitable for planting in a cutting frame, the method is simple to operate and easy to realize, has a good haploid induction effect, is high in induction efficiency, is rapid and accurate in identification, does not need to transplant all surviving seedlings, and saves time and subsequent identification steps.
Description
Technical Field
The invention belongs to the technical field of fruit and vegetable breeding, and particularly relates to a breeding method of white jade green cucumber.
Background
The new variety of cucumber 'jin Chun series' represented by jin Chun No. 4 cultivated in China in the 90 th century in 20 th century completes the update of high-yield and high-quality cucumber varieties in China, and then a series of excellent cucumber varieties are cultivated in succession all over the country, but the color of cucumber is mainly cyan, while the white cucumber mainly consumed in Yangtze river and yellow river basin before the 80 th century in China has the problems of low yield, susceptibility to downy mildew and the like, so that the research on the breeding of white cucumber by people is few, and nowadays, the new variety of cucumber with high quality, high yield and disease resistance is cultivated for enriching the flower color of cucumber.
However, the existing products have the problems of poorer disease resistance than the female parent, blight resistance, downy mildew and powdery mildew resistance, low bacterial angular spot resistance, unsuitability for planting in a cutting frame and unsuitability for planting in a cutting frame.
Therefore, the design of a breeding method of the white jade green cucumber is very important.
Disclosure of Invention
In order to solve the technical problems, the invention provides a breeding method of the white jade green cucumber, which aims to solve the problems that the existing product has poorer disease resistance than the female parent, is resistant to blight, downy mildew and powdery mildew, has low bacterial angular spot resistance, is not suitable for planting in a cutting frame and is not suitable for planting in a cutting frame.
A breeding method of white jade green cucumber comprises the following steps:
step S1: selecting male parent and female parent;
step S2: carrying out sexual hybridization;
step S3: f1 pollen is cultured in vitro to obtain haploid plants;
step S4: inducing chromosome doubling to obtain fertile homozygote;
step S5: and (3) breeding the white jade green cucumber meeting the conditions by a haploid breeding method.
Preferably, in step S1, the new variety 'baiyucui' of white cucumber, which is bred by the 12 th 31 th week-mouth vegetable research institute in 2015 and integrates disease resistance, high yield and high quality, is used as a female parent, the cucumber strips are straight, the cavity is small and the meat is thick, the skin is thin, crisp and sweet, and the cucumber is suitable for uncooked eating and cold mixing, and the 'baiyucui' cucumber 20B68-2-2-3 which is bred in 2007 and has stable characters and outstanding hybrid advantages is used as a male parent.
Preferably, in the step S2,
in the first step of hybridization, seeding male parent seedlings in the first ten days of 4 months, and seeding female parent seedlings in the middle ten days of 4 months, wherein the interval time between the seeding of the male parent and the seeding of the female parent is 10 days;
and secondly, respectively planting the male parent and the female parent, wherein the seed production density is 18-20 cm of plant spacing of the male parent, 20-25 cm of plant spacing of the female parent, and the number ratio of the male parent to the female parent is 1: 3.
And thirdly, the pollen of the male parent falls on the stigma of the pistil of the female parent, pollen grows out of a pollen tube which is a passage of the sperm and directly communicated to the ovule, and the sperm enters the ovule through the passage and is combined with the egg cell to finish fertilization, so that F1 is obtained.
Preferably, the chromosome doubling is induced by treatment with colchicine, the seeds with embryo germination are soaked for 8h with colchicine with the concentration of 0.6%, and the haploid doubling can be realized after the soaking is finished.
Preferably, in the step S5, when the roots of the flower culture regenerated plants grow to 4-6 and the roots grow to 3-5cm, the flower culture regenerated plants are moved to a greenhouse to be acclimatized by natural illumination, and the cap is not opened to acclimatize the seedlings for 3d and is opened to acclimatize the seedlings for 5 d; then, the flower culture seedlings are removed from the bottle, the culture medium attached to the roots is washed and then transplanted into a substrate, and conventional culture management is performed under appropriate conditions.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the white jade green cucumber product has the characteristics of excellent quality, crisp taste, small cavity, thick meat and the like, is bright green and white in appearance, has obvious and dense white spines, is straight in melon strips and uniform in thickness, has smaller leaves than female parents, has increased disease resistance compared with the female parents, and is resistant to blight, downy mildew and powdery mildew, and bacterial angular leaf spot.
2. The method is simple to operate and easy to realize, has a good haploid induction effect, high induction efficiency and quick and accurate identification, and plays an important role in cucumber haploid breeding; the technical breeding period is obviously shortened, the target character selection efficiency is obviously improved, a standard ploidy map can be established in the seedling stage, non-haploid plants are eliminated on a seedbed, all surviving seedlings do not need to be transplanted, and the time and subsequent identification steps are saved.
Detailed Description
The invention is further described below:
example 1:
the invention provides a breeding method of white jade green cucumber, which comprises the following steps:
step S1: selecting male parent and female parent;
step S2: carrying out sexual hybridization;
step S3: f1 pollen is cultured in vitro to obtain haploid plants;
step S4: inducing chromosome doubling to obtain fertile homozygote;
step S5: and (3) breeding the white jade green cucumber meeting the conditions by a haploid breeding method.
Preferably, in step S1, the new variety 'baiyucui' of white cucumber, which is bred by the 12 th 31 th week-mouth vegetable research institute in 2015 and integrates disease resistance, high yield and high quality, is used as a female parent, the cucumber strips are straight, the cavity is small and the meat is thick, the skin is thin, crisp and sweet, and the cucumber is suitable for uncooked eating and cold mixing, and the 'baiyucui' cucumber 20B68-2-2-3 which is bred in 2007 and has stable characters and outstanding hybrid advantages is used as a male parent.
In step S2, in the first step of hybridization, male parent seedlings are sown in the first ten days of 4 months, female parent seedlings are sown in the middle ten days of 4 months, and the time interval between the sowing of the male parent seedlings and the sowing of the female parent seedlings is 10 days; and secondly, respectively planting the male parent and the female parent, wherein the seed production density is 18-20 cm of plant spacing of the male parent, 20-25 cm of plant spacing of the female parent, and the number ratio of the male parent to the female parent is 1: 3. And thirdly, the pollen of the male parent falls on the stigma of the pistil of the female parent, pollen grows out of a pollen tube which is a passage of the sperm and directly communicated to the ovule, and the sperm enters the ovule through the passage and is combined with the egg cell to finish fertilization, so that F1 is obtained.
The induced chromosome doubling is processed by colchicine, the seed of embryo germination is soaked for 8h by using the colchicine with the concentration of 0.6%, and the haploid doubling can be realized after the soaking is finished.
In the step S5, when the root systems of the flower culture regenerated plants grow to 4-6 and the root length is 3-5cm, moving the plants to a greenhouse for hardening seedlings by using natural illumination, and hardening the seedlings for 3d without opening the bottle caps and hardening the seedlings for 5d by opening the bottle caps; then, the flower culture seedlings are removed from the bottle, the culture medium attached to the roots is washed and then transplanted into a substrate, and conventional culture management is performed under appropriate conditions.
The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention.
Claims (5)
1. A breeding method of white jade green cucumber is characterized by comprising the following steps:
step S1: selecting male parent and female parent;
step S2: carrying out sexual hybridization;
step S3: f1 pollen is cultured in vitro to obtain haploid plants;
step S4: inducing chromosome doubling to obtain fertile homozygote;
step S5: and (3) breeding the white jade green cucumber meeting the conditions by a haploid breeding method.
2. The breeding method of the white jade green cucumber as claimed in claim 1, wherein in step S1, the new variety 'white jade green' of white cucumber, which is bred by the institute for vegetables of 12-31 # 12-31-k in 2015 and integrates disease resistance, high yield and high quality, is used as a female parent, the cucumber strips are straight, the cavity is small and the meat is thick, the skin is thin, crisp and sweet, and is suitable for raw eating and cold mixing, and the 'white jade green' cucumber 20B68-2-2-3 with stable characters and outstanding hybrid advantages is bred in 2007 as a male parent.
3. The method for breeding white jade green cucumber as claimed in claim 1, wherein in step S2,
in the first step of hybridization, seeding male parent seedlings in the first ten days of 4 months, and seeding female parent seedlings in the middle ten days of 4 months, wherein the interval time between the seeding of the male parent and the seeding of the female parent is 10 days;
secondly, planting the male parent and the female parent respectively, wherein the seed production density is 18-20 cm of male parent plant spacing, 20-25 cm of female parent plant spacing, and the number ratio of the male parent to the female parent is 1: 3;
and thirdly, the pollen of the male parent falls on the stigma of the pistil of the female parent, pollen grows out of a pollen tube which is a passage of the sperm and directly communicated to the ovule, and the sperm enters the ovule through the passage and is combined with the egg cell to finish fertilization, so that F1 is obtained.
4. The breeding method of white jade green cucumber according to claim 1, wherein the induced chromosome doubling is treated with colchicine, and the seed of embryo germination is soaked with colchicine with concentration of 0.6% for 8h, so that haploid doubling can be realized after soaking.
5. The breeding method of the white jade green cucumber according to claim 1, wherein in the step S5, when the root system of the flower-cultivated regenerated plant grows to 4-6 strips and the root length is 3-5cm, the plant is moved to a greenhouse and acclimatized by natural illumination, and the cap is opened for acclimatization for 3d without opening the cap and is acclimatized for 5 d; then, the flower culture seedlings are removed from the bottle, the culture medium attached to the roots is washed and then transplanted into a substrate, and conventional culture management is performed under appropriate conditions.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911282809.7A CN112970577A (en) | 2019-12-13 | 2019-12-13 | Breeding method of white jade green cucumber |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911282809.7A CN112970577A (en) | 2019-12-13 | 2019-12-13 | Breeding method of white jade green cucumber |
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| CN112970577A true CN112970577A (en) | 2021-06-18 |
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| CN201911282809.7A Withdrawn CN112970577A (en) | 2019-12-13 | 2019-12-13 | Breeding method of white jade green cucumber |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1418950A (en) * | 2002-12-26 | 2003-05-21 | 北京市海淀区植物组织培养技术实验室 | Method for obtaining haploid plant strain by culturing anther or pollen |
| US20040055034A1 (en) * | 2002-09-18 | 2004-03-18 | Jahn Margaret M. | Multiple disease resistant early flowering cucumber variety |
| CN101317548A (en) * | 2008-07-18 | 2008-12-10 | 南京农业大学 | Cultivation method for Isolated microspore of cucumber |
| CN101658131A (en) * | 2009-09-17 | 2010-03-03 | 洛阳师范学院 | Method for breeding novel breed of cucumber |
| CN109220781A (en) * | 2018-10-13 | 2019-01-18 | 江苏强农农业技术服务有限公司 | A kind of Cucumber Target Leaf Spot resistance breeding method |
-
2019
- 2019-12-13 CN CN201911282809.7A patent/CN112970577A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040055034A1 (en) * | 2002-09-18 | 2004-03-18 | Jahn Margaret M. | Multiple disease resistant early flowering cucumber variety |
| CN1418950A (en) * | 2002-12-26 | 2003-05-21 | 北京市海淀区植物组织培养技术实验室 | Method for obtaining haploid plant strain by culturing anther or pollen |
| CN101317548A (en) * | 2008-07-18 | 2008-12-10 | 南京农业大学 | Cultivation method for Isolated microspore of cucumber |
| CN101658131A (en) * | 2009-09-17 | 2010-03-03 | 洛阳师范学院 | Method for breeding novel breed of cucumber |
| CN109220781A (en) * | 2018-10-13 | 2019-01-18 | 江苏强农农业技术服务有限公司 | A kind of Cucumber Target Leaf Spot resistance breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 赵鑫等: ""早熟高产白黄瓜新品种‘白玉翠’的选育"", 《中国瓜菜》 * |
| 陈芬芬等: ""黄瓜花蕾大小与花粉发育时期相关性研究"", 《延边大学农学学报》 * |
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Application publication date: 20210618 |