CN112931881A - Production method of green bean NMN small peptide powder and SOD soft capsule thereof - Google Patents
Production method of green bean NMN small peptide powder and SOD soft capsule thereof Download PDFInfo
- Publication number
- CN112931881A CN112931881A CN202110258425.2A CN202110258425A CN112931881A CN 112931881 A CN112931881 A CN 112931881A CN 202110258425 A CN202110258425 A CN 202110258425A CN 112931881 A CN112931881 A CN 112931881A
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- China
- Prior art keywords
- green bean
- nmn
- powder
- small peptide
- green
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A production method of green bean NMN small peptide powder and SOD soft capsules thereof belongs to biotechnology. The NMN small peptide SOD soft capsule comprises green bean NMN small peptide SOD tablets and linseed oil; the green bean NMN small peptide SOD tablet is obtained by tabletting according to the following proportion: 50-80% of NMN green bean extract powder, 10-30% of green bean small peptide powder, 5-15% of yeast SOD powder (20 ten thousand U/g), 4% of lactose, 0.5% of xylitol and 0.5% of magnesium stearate. The NMN green bean extract powder and the NMN green bean small peptide powder are obtained by centrifuging and filtering fresh green bean pods to obtain green bean filtrate and green bean filter cakes and further preparing the green bean filtrate and the green bean filter cakes. The invention extracts NMN from natural food, and obtains small peptide with molecular weight of 180-400 daltons by enzymolysis technology, so that the NMN can be protected and the stability of the NMN can be improved, and the product has strong health care effect.
Description
Technical Field
The invention relates to the technical field of NMN small peptide enzymolysis extraction and SOD soft capsule preparation, and belongs to the biotechnology.
Background
NMN (β -nicotinamide mononucleotide), a precursor of the cofactor NAD + of the long-life protein in the human body. NAD + is also called coenzyme I, is called Nicotinamide Adenine Dinucleotide (NADP), and participates in thousands of reactions in every cell. NAD + is an important coenzyme of tricarboxylic acid cycle, promotes the metabolism of sugar, fat and amino acid, and participates in the synthesis of energy; NAD + is in turn the sole substrate for coenzyme I consuming enzymes (the sole substrate for DNA repair enzyme PARP, the sole substrate for longevity protein Sirtuins, the sole substrate for cyclic ADP ribose synthase CD 38/157). Therefore, NAD + is very important for the human body and plays an important role in human body aging resistance. However, with age, NAD + levels in the human body are gradually decreasing, resulting in decreased mitochondrial activity, accelerated aging of mitochondria, cells, and even the whole body, and gradually entering the vicious circle, the human body gradually ages. Four classes of precursors that complement NAD + three metabolic cycles can be nicotinic acid, tryptophan, nicotinamide and NMN/NR. Compared with other supplementation modes of NAD +, NMN bypasses the bottleneck of NAMPT rate-limiting enzyme, and can rapidly supplement NAD + in vivo, so that the method is an optimal ideal supplementation method. The NAD + molecules are large, the NAD + directly supplemented from the outside hardly penetrates through cell membranes and enters the inside of cells, but the NMN molecules easily penetrate through the cell membranes and enter the inside of the cells. Once inside the cell, 2 NMN molecules will bind together to form one NAD + molecule. Therefore, the regular intake of NMN can effectively increase the content of NAD + in vivo, which is helpful for human to repair DNA damage by self, thereby achieving the purpose of delaying senility.
Beta-nicotinamide mononucleotide as intermediate in NAD + remedial pathway has the functions of resisting oxidation and reducing oxidative stress, and has good performance in the treatment of some specific diseases, such as cerebral apoplexy, cardiac ischemia-reperfusion, Alzheimer disease, Parkinson disease, acute kidney injury, retinal degenerative diseases, type II diabetes, and the like. Particularly in the aspect of anti-aging, NMN can slow down physiological decline of organisms, enhance energy metabolism and prolong the life. Because NMN is a human endogenous substance and has high safety, the NMN has wide prospect in the field of functional food development as an active substance.
At present, NMN is produced mainly by chemical synthesis, enzyme fermentation and conversion, etc. The products of the NMN method are inevitably doped with some chemical substances, and are difficult to apply to the fields of health care products and foods at present.
NMN is unstable in chemical property, very sensitive to light, oxygen and moisture, and often needs to be prepared at present. If the common preparation formulation is prepared by adopting the conventional auxiliary materials, the defects of poor stability, short shelf life and the like exist.
In order to solve the problem of unstable chemical property of NMN, chinese patent document CN109364091A discloses a method, which is to prepare a biopolymer nanosphere from NMN and a biopolymer carrier, and then prepare a pharmaceutically acceptable dosage form from the biopolymer nanosphere; chinese patent document CN110812365A discloses another method, that is, NMN is first adsorbed by ion exchange resin to form drug-loaded resin particles, and then the drug-loaded resin is adsorbed by coating material and plasticizer. The two methods are complex in process and use some unusual pharmaceutic adjuvants.
The small peptides are very active and often play a significant role in very small quantities; small peptides have important physiological functions. Relates to the fields of hormone, nerve, cell growth and reproduction of human body, and can regulate physiological functions of various systems and cells in the human body and maintain the normal of the systems of nerve, digestion, reproduction, growth, movement, metabolism, circulation and the like of the human body. The small peptide plays a key role in the growth, the metabolism, the diseases, the aging and the death of human beings. Small peptides are the most important active substances in the human body.
Disclosure of Invention
The invention aims at providing a method for directly extracting NMN small peptide from food green beans and a method for preparing capsule-in-capsule gel candy from the NMN small peptide, SOD and linseed oil (high content of alpha-linolenic acid).
To achieve the above object, the present invention adopts the following embodiments:
the raw materials used by the invention are fresh green bean pods, deep-sea hot spring yeast SOD powder and linseed oil, and the production steps are as follows:
(1) production of soybean milk
Peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: grinding the green beans into pulp by using a colloid mill;
ultrasonic extraction: adding water into the soybean milk, and carrying out ultrasonic extraction for 3 hours at 50 ℃ and 20000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, the discharging temperature is 90-110 ℃, and green bean NMN powder is obtained, wherein the NMN content is 1-10%;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bag to obtain powder of green bean NMN extract;
(3) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; firstly, adjusting the pH value of the soybean milk to 8.5-9.5 by using soda ash, heating to 50-55 ℃, stirring and activating for 2 hours; then adding alkaline protease accounting for 2 percent of the weight of the green beans, and stirring and reacting for 2 hours at 50-55 ℃; adding neutral protease accounting for 2% of the weight of the green beans, adjusting the pH value to be 6.5-7.5, and stirring and reacting for 2 hours at 50-55 ℃; then adjusting the pH value to be 4-6.5, adding bromelain accounting for 2% of the weight of the green beans or mixing the bromelain with papain to react for 8 hours at 50-75 ℃;
inactivating enzyme: heating to 95 ℃ for 10 minutes;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, and the discharging temperature is 90-110 ℃, so that the green soybean peptide powder is obtained;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bags to obtain a green bean small peptide powder finished product;
(4) green bean NMN small peptide SOD tablets:
tabletting by adopting the following proportion:
50-80% of NMN green bean extract powder,
10-30% of NMN green bean small peptide powder,
5 to 10 percent of yeast SOD powder (20 ten thousand U/g),
4 percent of lactose,
0.5 percent of xylitol,
magnesium stearate, 0.5%.
(5) NMN small peptide and SOD soft capsule production method thereof
Producing the capsule-in-capsule soft capsule according to the following mixture ratio:
green bean NMN small peptide SOD tablet, 0.33g,
linseed oil (containing alpha-linolenic acid, 70%) 0.6 g.
The invention has the beneficial effects that:
1. the invention provides a method for extracting NMN from natural food, and small peptides with the molecular weight of 180-500 daltons are obtained through an enzymolysis technology, so that the effects of protecting the NMN and improving the stability of the NMN can be achieved. Meanwhile, the health care function of the small peptide is increased.
2. In order to avoid the condition that the oral NMN is damaged by gastric acid when passing through the stomach, the invention uses deep-sea hot spring yeast SOD (superoxide dismutase) with high stability and high activity as a stabilizing agent and presses the deep-sea hot spring yeast SOD into NMN small peptide SOD tablets. The small peptide and the SOD can simultaneously protect the NMN from being decomposed by gastric acid and improve the gastric transit stability of the NMN. The strong antioxidant property of SOD also improves the whole anti-aging performance of the product.
3. The NMN small peptide SOD tablet adopts a capsule-in-capsule packaging method and is prepared into capsule-in-capsule gel candy by using linseed oil (high content of alpha-linolenic acid). When the soft capsule is taken orally, the shell of the soft capsule is decomposed in stomach, and the NMN small peptide SOD tablet enters the intestinal tract and is fully absorbed in the intestinal tract without being affected by gastric acid basically. The medium of the soft capsule contains a large amount of alpha-linolenic acid, is a health-care component for softening blood vessels and protecting heart, and has extremely strong health-care effect while protecting the NMN small peptide SOD tablet from being eroded by gastric acid.
The product of the invention is detected according to the corresponding method and steps of the national standard GB 5009.5-2016 protein, and the indexes meet the requirements of the standard.
Detailed Description
The following detailed description of the embodiments provided according to the present invention is given with reference to the following examples:
example 1
(1) Production of soybean milk
Selecting 500kg of high-quality full green bean pods;
peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: adding 3 times of purified water into semen Phaseoli Radiati kernel, and grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding 1 time of purified water into the green soybean milk, and carrying out ultrasonic extraction for 3 hours at 50 ℃ and 20000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) preparation of green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying in a spray drying tower at 170 deg.C to obtain coarse powder of NMN;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bag to obtain semen Phaseoli Radiati NMN extract powder with NMN content of 2.5%;
(3) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; adjusting pH of soybean milk to 9.5 with soda, heating to 55 deg.C, adding alkaline protease 2% of the weight of semen Phaseoli Radiati, and stirring at 55 deg.C for 2 hr; adding neutral protease 2 wt% of semen Phaseoli Radiati, adjusting pH to 7.5, and stirring at 50 deg.C for 2 hr; then adjusting the pH value to 5, adding bromelain or papain with the weight of 2 percent of that of the green beans, and stirring and reacting for 8 hours at 65 ℃;
inactivating enzyme: heating to 95 ℃ for 10 minutes;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying in a spray drying tower at 180 deg.C to obtain coarse powder of semen Phaseoli Radiati;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bags to obtain a green bean small peptide powder finished product; the molecular weight distribution of the green bean small peptide powder is more than 98% and less than 1000 daltons, wherein 60% is 180-500 daltons, and the weight average molecular weight is 310 daltons.
(4) Green bean NMN small peptide SOD tablets:
tabletting by adopting the following proportion:
NMN green bean extract powder, 60%
NMN green bean small peptide powder, 30%
Yeast SOD powder (20 ten thousand U/g), 5%
Lactose 4%
Xylitol 0.5%
Magnesium stearate, 0.5%;
(5) NMN small peptide and SOD soft capsule production method thereof
Producing the capsule-in-capsule soft capsule according to the following mixture ratio:
green bean NMN small peptide SOD tablet, 0.33g
Linseed oil (containing alpha-linolenic acid, 70%) 0.6 g.
Example 2
(1) Production of soybean milk
Selecting 500kg of high-quality full green bean pods;
peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: adding 3 times of purified water into semen Phaseoli Radiati kernel, and grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding 1 time of purified water into the green soybean milk, and carrying out ultrasonic extraction for 3 hours at 45 ℃ and 50000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) preparation of green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying in a spray drying tower at 160 deg.C to obtain coarse powder of semen Phaseoli Radiati NMN;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bag to obtain powder of green bean NMN extract; the NMN content is 1.8%;
(3) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; adjusting pH of soybean milk to 8.5 with soda, heating to 50 deg.C, adding alkaline protease 2% of the weight of semen Phaseoli Radiati, and stirring at 50 deg.C for 2 hr; adding neutral protease 2 wt% of semen Phaseoli Radiati, adjusting pH to 6.5, and stirring at 55 deg.C for 2 hr; then adjusting the pH value to 4, adding bromelain or papain with the weight of 2 percent of that of the green beans, and stirring and reacting for 8 hours at 50 ℃;
inactivating enzyme: heating to 90 deg.C for 10 min;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying in a spray drying tower at 160 deg.C to obtain semen Phaseoli Radiati small peptide powder;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bags to obtain a green bean small peptide powder finished product; the molecular weight distribution of the green bean small peptide powder is more than 97% and less than 1000 daltons, wherein 56% is 180-500 daltons, and the weight average molecular weight is 330 daltons.
(4) Green bean NMN small peptide SOD tablets:
tabletting by adopting the following proportion:
NMN green bean extract powder, 50%
NMN green bean small peptide powder, 30%
Yeast SOD powder (20 ten thousand U/g), 15%
Lactose 4%
Xylitol 0.5%
Magnesium stearate, 0.5%;
(5) NMN small peptide and SOD soft capsule production method thereof
Producing the capsule-in-capsule soft capsule according to the following mixture ratio:
green bean NMN small peptide SOD tablet, 0.33g
Linseed oil (containing alpha-linolenic acid, 70%) 0.6 g.
Example 3
(1) Production of soybean milk
Selecting 500kg of high-quality full green bean pods;
peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: adding 3 times of purified water into semen Phaseoli Radiati kernel, and grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding 1 time of purified water into the green soybean milk, and carrying out ultrasonic extraction for 3 hours at 55 ℃ and 30000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) preparation of green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying in a spray drying tower at 180 deg.C to obtain green bean NMN powder with NMN content of 3.5%;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bag to obtain powder of green bean NMN extract;
(3) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; adjusting pH of soybean milk to 9.0 with soda, heating to 53 deg.C, adding alkaline protease 2% of the weight of semen Phaseoli Radiati, and stirring at 53 deg.C for 2 hr; adding neutral protease 2 wt% of semen Phaseoli Radiati, adjusting pH to 7.0, and stirring at 53 deg.C for 2 hr; then adjusting the pH value to 6.5, adding bromelain which accounts for 2 percent of the weight of the green beans or mixing the bromelain with papain to react for 8 hours at 75 ℃;
inactivating enzyme: heating to 93 ℃ for 10 minutes;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying in a spray drying tower at 170 deg.C to obtain semen Phaseoli Radiati coarse powder;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bags to obtain a green bean small peptide powder finished product; the molecular weight distribution of the green bean small peptide powder is more than 99% and less than 1000 daltons, wherein 66% is 180-500 daltons, and the weight average molecular weight is 290 daltons.
(4) Green bean NMN small peptide SOD tablets:
tabletting by adopting the following proportion:
NMN green bean extract powder, 80%
NMN green bean small peptide powder, 10%
Yeast SOD powder (20 ten thousand U/g), 5%
Lactose 4%
Xylitol 0.5%
Magnesium stearate, 0.5%;
(5) NMN small peptide and SOD soft capsule production method thereof
Producing the capsule-in-capsule soft capsule according to the following mixture ratio:
green bean NMN small peptide SOD tablet, 0.33g
Linseed oil (containing alpha-linolenic acid, 70%) 0.6 g.
According to the embodiments, the method for extracting NMN from natural food is used, and small peptides with the molecular weight of 180-400 daltons are obtained through an enzymolysis technology, so that the effects of protecting NMN and improving the stability of NMN can be achieved. Meanwhile, the health care function of the small peptide is increased.
In order to avoid the condition that the oral NMN is damaged by gastric acid when passing through the stomach, the invention uses SOD (superoxide dismutase) with high stability and high activity as a stabilizing agent and presses the SOD into NMN small peptide SOD tablets. The small peptide and the SOD can simultaneously protect the NMN from being decomposed by gastric acid and improve the gastric transit stability of the NMN. The strong antioxidant property of SOD also improves the whole anti-aging performance of the product.
The NMN small peptide SOD tablet adopts a capsule-in-capsule packaging method and is prepared into capsule-in-capsule gel candy by using linseed oil (high content of alpha-linolenic acid). When the soft capsule is taken orally, the shell of the soft capsule is decomposed in stomach, and the NMN small peptide SOD tablet enters the intestinal tract and is fully absorbed in the intestinal tract without being affected by gastric acid basically. The medium of the soft capsule contains a large amount of alpha-linolenic acid, is a health-care component for softening blood vessels and protecting heart, and has extremely strong health-care effect while protecting the NMN small peptide SOD tablet from being eroded by gastric acid.
It should be further noted that the embodiments of the present invention do not limit the scope of the present invention, and all modifications that fall within the technical spirit and obvious technical solutions in the field shall fall within the scope of the present invention.
Claims (7)
1. An NMN small peptide SOD soft capsule is characterized in that: the composition is produced by the following components in proportion:
green bean NMN small peptide SOD tablet, 0.33g,
linseed oil, 0.6g,
wherein: the linseed oil contains 70 percent of alpha-linolenic acid;
the green bean NMN small peptide SOD tablet is obtained by tabletting according to the following proportion:
50-80% of NMN green bean extract powder,
10 to 30 percent of green bean small peptide powder,
5 to 15 percent of yeast SOD powder (20 ten thousand U/g),
4 percent of lactose,
0.5 percent of xylitol,
magnesium stearate, 0.5%.
2. The NMN small peptide SOD soft capsule as claimed in claim 1, wherein the following ratio is adopted for tabletting:
NMN green bean extract powder, 60%,
30 percent of green bean small peptide powder,
yeast SOD powder (20 ten thousand U/g), 5%,
4 percent of lactose,
0.5 percent of xylitol,
magnesium stearate, 0.5%.
3. The NMN small peptide SOD soft capsule as claimed in claim 1 or 2, wherein: the NMN green bean extract powder and the green bean small peptide powder are directly extracted from green beans serving as food.
4. The NMN small peptide SOD soft capsule as recited in claim 3, wherein: the NMN green bean extract powder and green bean small peptide powder are prepared by grinding fresh green bean pods with a colloid mill, extracting with ultrasonic extraction equipment, filtering with a centrifuge to obtain green bean filtrate and green bean filter cake, and further preparing the green bean filtrate or the green bean filter cake.
5. A method for producing green bean NMN small peptide powder for the NMN small peptide SOD soft capsule of claim 1 or 2, characterized in that:
(1) production of soybean milk
Peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding water into the soybean milk, and carrying out ultrasonic extraction for 3 hours at the temperature of 45-55 ℃ and 20000-ion 50000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, the discharging temperature is 90-110 ℃, and green bean NMN extract powder with the NMN content of 1-10% is obtained;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: and (3) packaging the powder in vacuum, and packaging by using an aluminum foil bag to obtain the green bean NMN extract powder.
6. A method for producing green bean NMN small peptide powder for the NMN small peptide SOD soft capsule of claim 1 or 2, characterized in that:
(1) production of soybean milk
Peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding water into the soybean milk, and carrying out ultrasonic extraction for 3 hours at the temperature of 45-55 ℃ and 20000-ion 50000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; firstly, adjusting the pH value of the soybean milk to 8.5-9.5 by using soda ash, heating to 50-55 ℃, stirring and activating for 2 hours; then adding alkaline protease accounting for 2% of the weight of the green beans, and stirring and reacting for 2 hours at 50-55 ℃; adding neutral protease accounting for 2% of the weight of the green beans, adjusting the pH value to be 6.5-7.5, and stirring and reacting for 2 hours at 50-55 ℃; then adjusting the pH value to be 4-6.5, adding bromelain accounting for 2% of the weight of the green beans or mixing the bromelain with papain to react for 8 hours at 50-75 ℃;
inactivating enzyme: heating to 90-95 ℃ for 10 minutes;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, and the discharging temperature is 90-110 ℃, so that the green soybean peptide powder is obtained;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: and (3) packaging the powder in vacuum, and packaging by using an aluminum foil bag to obtain a green bean small peptide powder finished product.
7. A production method of NMN small peptide SOD soft capsules is characterized in that:
(1) production of soybean milk
Peeling the beans: peeling green bean pods to obtain green bean kernels;
grinding soybean milk: grinding semen Phaseoli Radiati into slurry with colloid mill;
ultrasonic extraction: adding water into the soybean milk, and carrying out ultrasonic extraction for 3 hours at the temperature of 45-55 ℃ and 20000-ion 50000 HZ;
centrifugal filtration: filtering with a high-speed centrifuge to obtain green bean filtrate and green bean filter cake;
(2) green bean NMN extract powder
Fine filtering of filtrate: adopting a bamboo filter, and carrying out precision filtration by using diatomite to obtain an NMN extracting solution;
membrane refining and concentration: firstly, removing monovalent and divalent ions in the extract liquid by adopting a membrane filter to reduce the conductivity of the extract liquid; then, concentrating and dehydrating to obtain a concentrated NMN extracting solution;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, the discharging temperature is 90-110 ℃, and green bean NMN powder is obtained, wherein the NMN content is 1-10%;
screening: screening the obtained green bean NMN powder by adopting a 60-mesh ultrasonic vibration sieve, and collecting the powder which is screened by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bag to obtain powder of green bean NMN extract;
(3) preparation of green bean small peptide powder
Carrying out green bean filter cake enzymolysis reaction: adding the green bean filter cake into a reaction kettle, adding 5 times of water, and carrying out enzymolysis; firstly, adjusting the pH value of the soybean milk to 8.5-9.5 by using soda ash, heating to 50-55 ℃, stirring and activating for 2 hours; then adding alkaline protease accounting for 2% of the weight of the green beans, and stirring and reacting for 2 hours at 50-55 ℃; adding neutral protease accounting for 2% of the weight of the green beans, adjusting the pH value to be 6.5-7.5, and stirring and reacting for 2 hours at 50-55 ℃; then adjusting the pH value to be 4-6.5, adding bromelain accounting for 2% of the weight of the green beans or mixing the bromelain with papain to react for 8 hours at 50-75 ℃;
inactivating enzyme: heating to 90-95 ℃ for 10 minutes;
separation and filtration: separating bean dregs by using a butterfly centrifugal filter, and collecting filtrate; drying and recycling the bean dregs;
and (3) precise filtration: precisely filtering the filtrate by using a precise bamboo type diatomite filter to obtain clear and transparent green bean small peptide hydrolysate;
membrane refining and concentration: a membrane filter is adopted to remove univalent and divalent ions in the green bean small peptide hydrolysate and reduce the conductivity of the green bean small peptide hydrolysate; then concentrating and dehydrating to obtain concentrated green bean small peptide hydrolysate;
spray drying: spray drying is carried out by adopting a spray drying tower, the feeding temperature of the drying tower is 160-180 ℃, and the discharging temperature is 90-110 ℃, so that the green soybean peptide powder is obtained;
screening: sieving the obtained green soybean small peptide powder by using a 60-mesh ultrasonic vibration sieve, and collecting powder which is sieved by a 60-mesh sieve;
and (3) vacuum packaging: vacuum packaging the powder, and packaging with aluminum foil bags to obtain a green bean small peptide powder finished product;
(4) green bean NMN small peptide SOD tablets:
tabletting by adopting the following proportion:
50-80% of NMN green bean extract powder
10-30% of green bean small peptide powder
5-15% of yeast SOD powder (20 ten thousand U/g)
Lactose 4%
Xylitol 0.5%
Magnesium stearate, 0.5%;
(5) NMN small peptide and SOD soft capsule production method thereof
Producing the capsule-in-capsule soft capsule according to the following mixture ratio:
green bean NMN small peptide SOD tablet, 0.33g
Linseed oil (containing alpha-linolenic acid, 70%) 0.6 g.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1534098A (en) * | 2003-04-01 | 2004-10-06 | 成都辰农绿色产业有限公司 | technology of preparing polypeptide from soyabean waste dreg |
| CN111548382A (en) * | 2020-05-29 | 2020-08-18 | 广州颜如玉生物科技有限公司 | Method for extracting nicotinamide mononucleotide from avocado |
| CN112089700A (en) * | 2020-09-27 | 2020-12-18 | 朱洪滨 | NMN (N-methyl-N) -based multilayer composite tablet and preparation method thereof |
| CN112391436A (en) * | 2020-11-24 | 2021-02-23 | 中食都庆（山东）生物技术有限公司 | NMN-rich green soy peptide and preparation method and application thereof |
| CN112438917A (en) * | 2019-08-30 | 2021-03-05 | 广东丸美生物技术股份有限公司 | Cabbage extract and preparation method and application thereof |
-
2021
- 2021-03-10 CN CN202110258425.2A patent/CN112931881A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1534098A (en) * | 2003-04-01 | 2004-10-06 | 成都辰农绿色产业有限公司 | technology of preparing polypeptide from soyabean waste dreg |
| CN112438917A (en) * | 2019-08-30 | 2021-03-05 | 广东丸美生物技术股份有限公司 | Cabbage extract and preparation method and application thereof |
| CN111548382A (en) * | 2020-05-29 | 2020-08-18 | 广州颜如玉生物科技有限公司 | Method for extracting nicotinamide mononucleotide from avocado |
| CN112089700A (en) * | 2020-09-27 | 2020-12-18 | 朱洪滨 | NMN (N-methyl-N) -based multilayer composite tablet and preparation method thereof |
| CN112391436A (en) * | 2020-11-24 | 2021-02-23 | 中食都庆（山东）生物技术有限公司 | NMN-rich green soy peptide and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| （瑞典）哈纳斯（HANAS，R.）著: "《甜蜜一生由自己：1型糖尿病人自助全书》", 31 May 2012, 辽宁科学技术出版社 * |
| 家庭医药.快乐养生编辑部: "5种营养守护心脏健康", 《家庭医药.快乐养生》 * |
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