KR101485575B1 - An method of Extracts for A GABA ingredient from germinated brown rice and that of using health function composition for a diabetic - Google Patents

Abstract

The present invention relates to a method for extracting soluble GABA components from germinated brown rice and a health functional composition using the same. The present invention relates to a process for producing a germinated brown rice extract, which comprises adding water at a temperature of 75 ° C to 100 ° C to a germinated brown rice germinated brown rice powder at 10 to 15 times the germinated brown rice weight (l / kg) A first step of cooling the temperature of the next extract to 70 캜; Glutamic acid decarboxylase corresponding to 3% by weight of dry germinated brown rice and α-amylase, 3% by weight of dry weight of germinated brown rice, were added to the extraction tank and fermentation temperature was 70 ° C. and fermentation time was 6 A second step of fermenting the extract with time; In order to remove solids from germinated brown rice fermented extract, the solid content was separated using a centrifugal dehydrator or a filter press. Then, in order to remove insoluble microparticles, proteins and macro carbohydrates in the extracted fermentation broth, ; In order to remove the inactive polymer carbohydrate and bacterial microorganism of the extracted primary purified solution, an Ultra filtering system equipped with a TFF (Tangential Flow Filtration) type membrane was separated by retentate and permeate, and then a germinated brown rice refining solution permeated A fourth step of extraction; And a fifth step of adding the extracted germinated brown rice second refinement liquid to a vacuum concentrator, concentrating the concentrated germinated brown rice solution until the degree of concentration reaches 20 brix or more, or highly concentrating the solution to 65 brix or more to extract GABA purified solution.
As a result, it can be applied to food and commercial as a health functional food containing GABA component extract by utilizing a high purity extract containing GABA component of germinated brown rice as a main component, thereby suppressing the tendency to develop adult diseases which spread to lower age layers do.

Description

[0001] The present invention relates to a method for extracting soluble GABA components from germinated brown rice and a health functional composition for diabetic patients using the same,

The present invention relates to a method for extracting soluble GABA components from germinated brown rice and a health functional composition for diabetic patients using the same. More specifically, the present invention relates to a method for extracting GABA (gamma -aminobutyric acid) Extracting the GABA component from germinated brown rice which can provide a health food having the effect of removing cholesterol or improving diabetes as a health functional food, and a health functional composition for diabetic patients using the same.

Modern society has brought about many changes in diet due to rapid industrial development. In particular, our human body, which has been dominated by instant foods and synthetic seasonings, has suffered from a wide variety of adult diseases, such as diabetes, hypertension, hyperlipemia, arteriosclerosis, obesity, and dementia, And the consumption of rice is continuously decreasing due to such eating habits.

GABA (γ-Aminobutyric acid) is a kind of nonspecific amino acid widely distributed in nature and is an excitatory inhibitory neurotransmitter. It accounts for about 30% of all neurotransmitters in the central nervous system and exists at a high concentration of about 200 to 1,000 times that of other neurotransmitters.

GABA is found in the brain, kidneys, heart, and lungs in animals, and in plants, germinated brown rice, germinated grains, green tea, and Chinese cabbage roots. GABA is known to be involved in the regulation of many physiological mechanisms of the human body such as inhibition of blood pressure rise, inhibition of blood cholesterol and triglyceride increase, improvement of blood flow in the brain, prevention of obesity, improvement of visual acuity, It is known that it is also involved in the regulation of the secretion and also has the effect of alleviating pain.

As a result of this diet, rice consumption is steadily decreasing. In this context, we have developed a production process technology capable of mass production of GABA, which is the most abundant GABA in germinated brown rice. The main ingredient of this product is GABA extract, which improves diabetes and eliminates cholesterol There is a growing need for the development of health foods.

[Related Technical Literature]

1. Method for manufacturing germinated brown rice with enhanced gabar component and method for producing germinated brown rice with enhanced gabar component (Patent No. WO2005 / 030510) Application No. 10-2004-0008491)

2. A mainstream composition containing a large amount of gabas and functional polysaccharides using germinated brown rice and kelp extract and a process for producing the same (Japanese Patent Application Laid-Open Patent Application No. 10-2007-0084885)

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above problems and it is an object of the present invention to provide a health functional food containing GABA component extract by utilizing a high purity extract containing GABA component of germinated brown rice as a main component, A method for extracting soluble GABA components from germinated brown rice and a health functional composition for diabetic patients using the same.

In addition, it is possible to increase the consumption of rice by making it possible to develop the agroeconomic economy, which is getting harder due to the decrease of rice consumption in the free trade agreement between the countries and the dietary life, Soluble GABA component of germinated brown rice and a health functional composition for diabetic patients using the same.

However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to accomplish the above object, the present invention provides a method for extracting soluble GABA components from germinated brown rice, which comprises adding ginseng brown rice weight (l / kg) of water between 75 ° C and 100 ° C to dried powdered germinated brown rice, And the mixture is stirred for 2 to 6 hours to obtain a water-soluble germinated brown rice extract, followed by cooling the extract to a temperature of 70 캜; Glutamate decarboxylase (GAD) equivalent to 3% by weight of dry weight of germinated brown rice and? -Amylase, which is 3% by weight of dry weight of germinated brown rice, were added to an extraction tank and fermentation temperature A second step of fermenting the extract at 70 DEG C and a fermentation time of 6 hours; In order to remove solids from germinated brown rice fermented extract, the solid content was separated using a centrifugal dehydrator or a filter press. Then, in order to remove insoluble microparticles, protein and macro carbohydrates in the fermentation broth, ; After the third step, an ultra filtration system equipped with a membrane of TFF (Tangential Flow Filtration) method was used to remove the inactive polymer carbohydrate and the bacterial microorganism of the extracted first purified liquid A fourth step of extracting a germinated brown rice secondary refining liquid which is separated into a retentate and a permeate and is then permeated; And a fifth step of introducing the extracted germinated brown rice secondary purified solution into a vacuum concentrator, concentrating the concentrated germinated brown rice solution to a concentration of 20 brix or more after concentration, or extracting the purified GABA solution with high concentration of 65 brix or more .

In this method, after the fourth step, the extracted germinated brown rice second refinement liquid is poured into a vacuum concentrator, and after decompression, the germinated brown rice GABA The purified component extract is added to a freeze-dryer, and the mixture is heated to -40 캜 for 36 hours by freeze-drying at -60 캜 to extract GABA purified powder.

In addition, the method for extracting soluble GABA components of germinated brown rice according to the present invention is characterized in that, after the second step is performed, the temperature of the extract solution after the fermentation process is raised to 95 ° C or higher, and then cooling water is immediately introduced into the jacket of the extraction tank, To stop the activity of the glutamate decarboxylase and the? -Amylase.

In addition, the method for extracting soluble GABA component of germinated brown rice according to the present invention is characterized in that in the fourth step, in order to obtain the maximum GABA component of the germinated brown rice in the first purified solution retentate, And further comprising the step of adding and diluting the purified water when the flow rapidly increases and repeating the filtration process several times.

The method for extracting soluble GABA component of germinated brown rice according to the embodiment of the present invention and the health functional composition for diabetic patients using the germinated brown rice according to the present invention is a health functional food containing GABA component extract by utilizing a high purity extract containing GABA component of germinated brown rice as a main ingredient, And it can be applied commercially to control the trend of adult diseases such as diabetes which spread to lower age groups and can provide a great help to improve the quality of life in an aged society.

In addition, according to another embodiment of the present invention, the method for extracting soluble GABA components from germinated brown rice and the health functional composition using the same can be applied to the agricultural economy which becomes increasingly difficult due to the reduction of rice consumption in the free trade agreement and the eating habits of the germinated brown rice It is possible to increase the consumption of rice by making it possible to develop it by combining it with beneficial health functional food, thereby providing an effect to activate the agricultural economy.

1 is a flowchart of a method for extracting soluble GABA components from germinated brown rice according to the present invention
FIG. 2 is a table showing efficacy comparison results of the germinated brown rice extract according to the present invention and the untreated enzyme

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a detailed description of preferred embodiments of the present invention will be given with reference to the accompanying drawings. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

The present invention will be more specifically illustrated by the following Examples and Experimental Examples. However, these examples and experimental examples are merely examples of the present invention and do not limit the scope of the present invention.

≪ Example 1 >

 Production method of water-soluble extract containing GABA as a main component in germinated brown rice

1, a water-soluble GABA component extraction method for germinated brown rice of the present invention will be described.

(1) First, the dried germinated brown rice is roughly pulverized, and then the germinated brown rice dried weight (l / kg) ratio of water at 75 ° C to 100 ° C is adjusted to about 10 to 15 times the extraction condition suitable for the physical properties of the GABA component And stirred for 2 to 6 hours to obtain an aqueous germinated brown rice extract, followed by cooling the extract to a temperature of 70 ° C (see step S10)

(2) Glutamic acid decarboxylase (GAD) corresponding to 3% by weight of dry weight of germinated brown rice used in hot water extraction and α-amylase, which is 3% by weight of dry weight of germinated brown rice, Is put into an extraction tank and the fermentation temperature is set to 70 캜 and the fermentation time is set to 6 hours to ferment the germinated brown rice extract (refer to step S20)

(3) After the fermentation process of the germinated brown rice extract is completed, the temperature of the extract is raised to 95 ° C or higher, and cooling water is immediately put into the jacket of the extraction tank to quench the fermented liquid to stop the activity of glutamate decarboxylase and α-amylase (Refer to step S30)

(4) In order to remove solids from germinated brown rice extract, fermentation broth was separated by using centrifugal dehydrator or filter press. Then, centrifugal dehydrator or filter press was used to remove the insoluble microparticles, protein and macro carbohydrate in the fermentation broth. To extract germinated brown rice water-soluble primary purification liquid at a rate of 15,000 to 20,000 RPM (see step S40).

(5) In order to remove the inactive polymer carbohydrates and microorganisms in the purified purified germinated brown rice and to increase the content of GABA components, it was passed through ultra-high-speed centrifuge, and TFF (Tangential Flow Filtration ) Was separated into a retentate and a permeate by using an ultrafiltering system equipped with a membrane type 5KDa-pore size membrane, and then germinated brown rice was permeated Only the second purification solution is extracted (refer to step S50)

  On the other hand, in order to maximize the GABA content of the germinated brown rice in the first purified liquid which is retentated during the extraction of the second purified liquid, when the concentration of the retentate is increased and the filtration pressure significantly increases and the flow rapidly decreases, the purified water is diluted The secondary filtrate may be extracted by repeating the post-filtration process several times.

(6) The germinated brown rice secondary purified solution extracted by the above procedure is put into a vacuum concentrator, and the vacuum is concentrated until the degree of concentration reaches 20 brix or more, or the concentration is 65 brix or more to extract the GABA purified solution (refer to step S60) )

Further, as an additional feature of the present invention, the extracted germinated brown rice secondary purified solution is put into a vacuum concentrator, and after decompression, the GABA component extracted and refined liquid concentrated from the germinated brown rice at a concentration of 20 brix is put into a freeze dryer, And the mixture is then lyophilized by raising the temperature to 40 ° C for 36 hours to extract the GABA purified powder (see step S70)

In another aspect of the present invention, the purified solution or the purified powder of the water-soluble GABA component of germinated brown rice extracted by the above procedure can be subjected to enzymatic fermentation, which is a process in which the GABA component- Amylase or glutamate decarboxylase or α-amylase and glutamate decarboxylase were added to the extract, regardless of the dry weight of the germinated brown rice, and the fermentation time was set at 30 to 80 ° C. for 2 to 24 hours, To increase the content of the GABA component.

≪ Example 2 >

A method of manufacturing a health food having the effect of eliminating cholesterol containing GABA component or improving diabetes

The efficacy of the manufacturing process of a health drink food having the effect of removing GABA component-containing cholesterol or diabetes by using the purified liquid or purified powder prepared in Example 1 is as follows. GABA component extraction from germinated brown rice The purified solution was mixed with other raw materials as shown in Table 1 below, mixed with purified water in a mixing tank, stirred until completely dissolved, steam was then fed into the double jacket of the mixing tank for 2 hours to sterilize Whereby a food for beverages was prepared.

Raw material name content(%) Extract of GABA from germinated brown rice (30Brix) 20 Guava leaf extract (27%) Suitable amount Ovarian Maltodextrin (85%) Suitable amount Palatinos Suitable amount Cyclodextrin Suitable amount Vitamin C Suitable amount Vitamin B1 hydrochloride Suitable amount Vitamin B2 Suitable amount Vitamin B6 hydrochloride Suitable amount Purified water Suitable amount

≪ Example 3 >

Comparison of efficacy according to GABA content of germinated brown rice extract according to enzyme treatment and enzyme untreated process

(1) GABA content comparison experiment method

① germinated brown rice extract 5 g of dried powder was filtered with 0.02 N HCl and filtered through a 0.45 μm membrane filter.

② GABA content was measured by HPLC under the conditions shown in the table below.

Time (min) Solvent composition (%) Solventa Solvent B 0 95 5 31 44 56 33 44 56 34 0 100 40 0 100

_{Solvent A: 40mM NaH 2 PO 4} (pH 7.8)

    Solvent B: ACN: MeOH: D.W (45: 45: 10)

③ The column used for the analysis was Capcellpak UG120 C ₁₈ (250 x 4.6 mm, 5 ㎛), flow rate was set to 1.5 ml / min, 10 μl was injected using Autosampler, and the UV detector was measured at 338 nm The standard material was imported from GABA (Sigma Co. Ltd.).

(2) Comparison of GABA content

    GABA content comparison test result table

Classification (average of 3 experiments) GABA content (/ / g) Enzyme untreated 138.5 Enzyme treatment 245.0

As shown in FIG. 2, the fermentation process using α-amylase and glutamic acid decarboxylase in the extraction process of germinated brown rice GABA component was carried out as follows. The content of GABA component was found to be 77% higher than that of the fermentation process.

<Experimental Result 1>

Effect of dietary supplement of GABA

(1) Experimental method for improving diabetes

   - Four diabetic men and two adult women were treated with the GABA ingredient-containing health food prepared according to Example 2, four times daily, 30 minutes after lunch and dinner, and before bedtime, and 150 ml It was steadily ingested for three months.

- All the experimental groups were taking medication prescribed by the diabetes prescription from the hospital for over 1 year. In this experiment, the prescription treatment was kept as it is, and the additional food containing GABA ingredient was taken under the same condition as above.

(2) Experimental results of improving diabetes

No gender Blood sugar
(Before ingestion) Symptom dosage
(Ml / day) term Blood sugar
(After ingestion) effect
(Survey results) One female 80-300 Thirsty 600 3M 140-150 Improving 2 south 300 Insomnia, irritability,
Decreased visual acuity 600 3M 108 Improving 3 female 300-400 - 600 3M 120 Improving 4 south - Fatty liver 600 3M - Improving 5 south 260 (400) 7 kg weight loss, blood pressure lowering agent insulin 7 units 600 3M 140 1400 calories No insulin needed 6 south Seven years of stress 20kg weight loss, insulin 600 3M - Improving

According to the results of this experiment, gabapentinized GABA-containing health food was consumed 150 ml once a day for 4 consecutive 3 months for diabetic patients (fasting blood glucose level 200 ~ 300 ㎎ / ㎗) The results showed that the fasting blood glucose level was 80 ~ 100mg / dl and the blood glucose level was 100 ~ 200mg / dl, which is the limit value. .

Therefore, it was determined that the health food prepared with the composition containing the germinated brown rice GABA component of the present invention had an effect of improving diabetes.

<Experimental Result 2>

The method for extracting soluble GABA component of germinated brown rice and the health functional composition using the germinated brown rice of the present invention is a kind of amino acid which is a non-monocarboxylic acid signaling substance of GABA (γ-Aminobutyric acid) By improving blood sugar level by promoting consumption, it is possible to improve the diabetes and to promote the decomposition of cholesterol and triglyceride, and to improve adult diseases such as obesity and hypertension.

In addition, it can be confirmed that the GABA component of the present invention contains about 77% or more of the GABA component from the germination labyrinth through the production method of extracting the GABA component in high purity from the germinating labyrinth through the fermentation process using the enzyme, Of diabetes mellitus. The results showed that the blood glucose level was significantly improved in all the subjects, and the subjects of all subjects were directly experiencing the complication rate due to diabetes mellitus , Respectively.

In addition, it can be applied to all foods including beverages, health food, confectionery, liquor, etc., through cholesterol removal efficacy, which is an effect of germinated brown rice, and water extractable refining materials based on GABA ingredient of germinated brown rice to improve diabetes .

In addition, an extractive refinement material based on the water-soluble GABA component of germinated brown rice produced based on the production technique described in Example 1 above is used as a raw material medicine and a general medicine product of various formulations for the purpose of improving diabetes and cholesterol- .

According to an embodiment of the present invention, the present invention can be applied by a person skilled in the art to a range including cereals such as brown rice, white rice, black rice, and barley and wheat as well as germinated brown rice.

As described above, preferred embodiments of the present invention have been disclosed in the present specification and drawings, and although specific terms have been used, they have been used only in a general sense to easily describe the technical contents of the present invention and to facilitate understanding of the invention , And are not intended to limit the scope of the present invention. It is to be understood by those skilled in the art that other modifications based on the technical idea of the present invention are possible in addition to the embodiments disclosed herein.

Claims (5)

Water from 75 ° C to 100 ° C was added to the germinated brown rice weight (l / kg) ratio 10 to 15 times and the mixture was agitated for 2 to 6 hours to obtain a water-soluble germinated brown rice extract. A first step of cooling the temperature to 70 占 폚;
Glutamate decarboxylase (GAD) equivalent to 3% by weight of dry weight of germinated brown rice and? -Amylase, which is 3% by weight of dry weight of germinated brown rice, were added to an extraction tank and fermentation temperature A second step of fermenting the extract at 70 DEG C and a fermentation time of 6 hours;
In order to remove solids from germinated brown rice fermented extract, the solid content was separated using a centrifugal dehydrator or a filter press. Then, in order to remove insoluble microparticles, protein and macro carbohydrates in the fermentation broth, ;
After the third step, an ultra filtration system equipped with a membrane of TFF (Tangential Flow Filtration) method was used to remove the inactive polymer carbohydrate and the bacterial microorganism of the extracted first purified liquid A fourth step of extracting germinated brown rice secondary purified liquid which is separated into a retentate and a permeate and is then permeated;
And a fifth step of introducing the extracted germinated brown rice secondary purified solution into a vacuum concentrator and concentrating the resulting solution until the concentration of the solution reaches 20 brix or more after the decompression or increasing the concentration to 65 brix or more to extract the GABA purified solution A Method for Extracting Soluble GABA Components from Germinated Brown Rice.
The method according to claim 1,
After the fourth step,
The extracted germinated brown rice secondary purified solution was put into a vacuum concentrator, and after decompression, the GABA component extracted and purified from the germinated brown rice, which had been concentrated to a concentration of 20 brix, was added to the freeze drier and lyophilized by heating at -60 ° C to 40 ° C for 36 hours And extracting the purified GABA powder.
The method according to claim 1,
After the second step,
Characterized in that the temperature of the extract solution after the fermentation process is raised to 95 ° C or more and then cooling water is put into the jacket of the extraction tank to quench the extracted fermentation broth to stop the activity of glutamate decarboxylase and α-amylase, Component extraction method.
The method according to claim 1,
In the fourth step, in order to maximize the GABA content of the germinated brown rice in the primary purification liquid to be the retentate, the filtration pressure significantly increases as the concentration of the retentate solution increases, Further comprising the step of adding diluted water to the filtrate and repeating the filtration process several times when the pH of the germinated brown rice falls rapidly.
A health functional composition for diabetic patients comprising the active ingredient of the water-soluble GABA extract of germinated brown rice extracted through the process of claim 1 for removing cholesterol or improving diabetes

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