CN1534098A - technology of preparing polypeptide from soyabean waste dreg - Google Patents
technology of preparing polypeptide from soyabean waste dreg Download PDFInfo
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- CN1534098A CN1534098A CNA031175864A CN03117586A CN1534098A CN 1534098 A CN1534098 A CN 1534098A CN A031175864 A CNA031175864 A CN A031175864A CN 03117586 A CN03117586 A CN 03117586A CN 1534098 A CN1534098 A CN 1534098A
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Abstract
A process for preparing polypeptide from soybean dregs includes proportionally mixing soybean dregs, deionized water, and composite proteinase, enzymlyzing at 40-60 deg.C for 5-24 hr, regulating pH=4-4.5, removing unhydrolyzed protein deposit, bitter taste, color and salt, and classifying and concentrating. Its advantages are low cost and productivity. Its product features high adaptability to acid, alkali and cells, high thermal stability, flowability and absorptivity, and no hypersensitivity.
Description
Affiliated technical field the invention belongs to the biological products manufacture field, especially relates to a kind of technology for preparing polypeptide from the soybean waste residue.
Background technology China soybean is produced about 1,640 ten thousand tons per year, accounts for 9% of Gross World Product, occupies the 3rd.Soybean is as a kind of important oil crops, its output be used for grease production more than 80%, so just produced a large amount of sex change dregs of beans.Along with the continuous progress of science and technology and people further investigation to soybean nutritional effect and health-care effect, multiple trace ingredients relation bioactive with it has been subjected to people's attention in the soybean, wherein extracting soybean isoflavones from soybean has become the importance in soybean deep processing field, and has formed suitable industrialized scale.According to incompletely statistics, annual about 150 tons of the soybean isoflavones of producing of China produces 30,000 tons dregs of beans slag approximately.Because also do not set up the processing channel of reliable, the economically viable recycling of technology, high value conversion at present, therefore a large amount of dregs of beans slags has only small part to be used for leavened food production mainly as feed.This has not only caused the huge waste of resource but also can bring a large amount of environmental problems.
Soybean polypeptide have easily digest and assimilate, can be rapidly to body energy, no protein denaturation, free from beany flavor, no residue are provided, molecular weight is little, soluble in water and also do not produce precipitation under acidic conditions, solution viscosity is little and be heated and characteristic such as do not solidify, and is a kind of more satisfactory novel soybean deep processed product.And exciting be that soybean polypeptide is a kind of active polypeptide, have and reduce serum cholesterol, hypotensive and promote special physiological properties such as metabolism of fat, in foodstuffs industry, have very widely purposes and than the wide development application prospect as functional factor.
The biologically active function is the key property of soybean polypeptide, and the biological activity of soybean polypeptide depends primarily on its specific three-dimensional arrangement and conformation.In case after three-dimensional arrangement that these are specific and conformation are destroyed, polypeptide will lose activity.Existing protease method prepares soybean polypeptide technology, the inefficiency of protolysate raw material, and also required time is long; And the technology that conventional acid hydrolysis and alkali hydrolysis method prepare soybean polypeptide, though the efficient height of protolysate raw material, but its inherent three-dimensional arrangement and conformation are suffered serious destruction to a great extent, and some amino acid may be decomposed simultaneously, thereby makes product lose biological activity; Acid hydrolysis and basic hydrolysis technology also can be brought environmental pollution in addition.
The soybean polypeptide of the dregs of rice slag preparation behind the soybean isoflavones is extracted in utilization, and its product is the mixture of the different polypeptide of molecular weight, and the polypeptide of different molecular structures and molecular size has different biological activitys and function, and its application also has nothing in common with each other.If can utilize modern separation means, isolate the active polypeptide of different fractions, with its further deep processing, be widely used in fields such as nutritive health-care, medical science pharmaceuticals and beauty and skin care, its social benefit, economic benefit will be more remarkable.
The objective of the invention is to overcome the defective of prior art, provide a kind of technology that from the soybean waste residue, prepares polypeptide, to prepare natural radioactivity peptide with different biological functions; guarantee the activity of native peptides to greatest extent; running cost is low, the production efficiency height, and help environment protection.
Summary of the invention the objective of the invention is to realize by following technical proposals:
With after extracting 0.2～1.0 part of 100～300 parts of 100 parts in soybean waste residue, deionized waters, polynary compound protease behind the soybean isoflavones and stirring, in pH=5～7,30～60 ℃ of following enzyme digestion reactions of temperature 5～24 hours; The pH value of re-adjustment enzymolysis solution is 4.0～4.5, removes unhydrolysed protein precipitation, obtains soybean polypeptide mixed solution crude product.
0.5%～1.5% times of gac that adds the polypeptide quality in soybean polypeptide mixed solution crude product time stirred 10～30 minutes at 30～40 ℃, pH=2.0～4.0, filtered 2～3 times; The filtrate H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-, obtain soybean polypeptide mixed solution elaboration.
Soybean polypeptide mixed solution elaboration is removed particulate by the millipore filtration of 0.22 μ m; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the soybean polypeptide mixed solution is carried out classification and concentrate, the red-tape operati temperature is at 20～40 ℃, pressure 0.1～0.25Mpa, pH=5～7,2～5 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, obtains the soybean polypeptide concentrated solution of different fractions after ultrafiltration.
The soybean polypeptide concentrated solution of above-mentioned soybean polypeptide mixed solution crude product or soybean polypeptide mixed solution elaboration or each fraction carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain the powder soybean polypeptide of crude product powder soybean polypeptide or elaboration powder soybean polypeptide or various fractions.
In the such scheme, the soybean waste residue that extracts behind the soybean isoflavones is used further to enzyme digestion reaction after pre-treatment, and pretreatment technology is: will extract the soybean waste residue behind the soybean isoflavones, and 90 ℃ of heating 5～20 minutes down, lower the temperature immediately, and clean, defibrination, coarse filtration.
In the such scheme, 100～300 parts of 100 parts in soybean waste residue, deionized waters extracting behind the soybean isoflavones are regulated pH=5～7 with acid or alkali, be warming up to 30～60 ℃, insulated and stirred added polynary compound protease again and carries out enzyme digestion reaction after 10～30 minutes.
In the such scheme, with the soybean waste residue that extracts behind the soybean isoflavones, 90 ℃ of heating 10 minutes down, cooling is immediately cleaned, defibrination, coarse filtration handle; Through above-mentioned pretreated 100 parts of soybean waste residues and 100～300 parts of deionized waters, add in the biochemical reactor; Regulate pH=5～7 with acid or alkali, be warming up to 30～50 ℃, insulated and stirred 10～30 minutes; Add 0.2～1.0 part of polynary compound protease in biochemical reactor, after stirring, enzyme digestion reaction 5～24 hours; PH value by the adjusting protein enzymatic hydrolyzate is 4.0～4.5, removes unhydrolysed protein precipitation; Add 0.5%～1.5% times of gac of polypeptide quality in the hydrolysate of soybean protein liquid, time stirred 10～30 minutes, filter 2～3 times at 30～40 ℃, pH=2.0～4.0; With the hydrolysate of soybean protein liquid H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-Remove particulate by the millipore filtration of 0.22 μ m again; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the polypeptide behind the enzymolysis is carried out classification and concentrate, the red-tape operati temperature is at 20～40 ℃, pressure 0.1～0.25Mpa, pH=5～7,2～5 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, obtains the soybean polypeptide concentrated solution of different fractions after ultrafiltration; The soybean polypeptide concentrated solution of the different fractions that obtains is carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain the finished product powder soybean polypeptide of different fractions.
In the such scheme, polynary compound protease is that microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase are by equal mixed.
In the such scheme, microbial protease is an isolated microbial protease from tempeh.
The present invention has following advantage:
(1) waste residue behind the utilization extraction soybean isoflavones prepares soybean polypeptide for raw material, make waste material obtain effective utilization, and whole process is friendly to environment;
(2) before the enzyme digestion reaction, pretreated dregs of beans was heated 10 minutes down at 90 ℃, both can prevent that the soy bean proteinous soln viscosity from raising, and can improve its degree of hydrolysis again greatly;
(3) isolated microbial proteinous endonuclease capable acts on the higher structure of soybean protein effectively from tempeh, so this enzyme has good enzymolysis to soybean protein;
(4) site of endonuclease reaction is effectively controlled in multienzyme synergism effect, obtains target product;
(5) multi-enzymatic hydrolysis carries out under comparatively gentle condition, can guarantee to deposit the nutritive value of soybean polypeptide and farthest active the recovery;
(6) at the appearance of soybean protein hydrolysate bitter taste, on the basis of adopting the multienzyme synergism hydrolysis, cooperate charcoal absorption to handle, make the soybean polypeptide taste be improved significantly, the transparent color and luster clarification;
(7) the employing ion-exchange-resin process removes the Na in the hydrolyzed solution
+And Cl
-, greatly reduce salt content, eliminated the saline taste of hydrolyzed solution;
(8) adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, no phase transformation takes place, and no pyrogen imports, and not with an organic solvent, does not change pH value of solution value and ionic strength, and these all help the active recovery of soybean polypeptide;
(9) purposes different according to soybean polypeptide can freely be selected the parameter of the molecular weight cut-off of ultra-filtration membrane, thereby obtain the product of corresponding molecular weight ranges;
(10) used microbial protease and animal and plant proteolytic enzyme is safe and reliable in the biochemical reaction process, so its product is green.
The present invention adopts from tempeh isolated microbial protease to handle the waste residue that extracts behind the soybean isoflavones in conjunction with animal and plant protease; provide the natural radioactivity peptide of different biological functions by ultra-filtration technique classification preparation; guaranteed the activity of native peptides to greatest extent; running cost is low; the production efficiency height, and help environment protection.Product has the series of features such as adaptability of flowability, high-absorbable, nonallergic and cell under good soda acid adaptability, thermostability, the higher concentration, can be widely used in nutritive health-care, fields such as medical science pharmaceuticals and beauty and skin care will produce significant social and economic benefit.
Embodiment is carried out concrete description below in conjunction with embodiment to the present invention, but the present invention is not limited only to described embodiment.
Embodiment one
With the soybean waste residue that extracts behind the soybean isoflavones, 90 ℃ of heating 10 minutes down, cooling is immediately cleaned, processing such as defibrination separation, fine grinding, filtration; Dregs of beans slag double centner homogenate with pretreated mixes for 200 kilograms with deionized water, joins in the biochemical reactor; With acid for adjusting pH=5, be warming up to 45 ℃, insulated and stirred 20 minutes; 0.25 kilogram of the polynary compound protease that adding is formed by equal mixed by microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase in biochemical reactor, after stirring, enzyme digestion reaction 12 hours; PH value by the adjusting protein enzymatic hydrolyzate is 4, removes unhydrolysed protein precipitation; At carbon: liquid=1: 100,40 ℃ of temperature under the condition of pH=4, add gac, stir 20 minutes, filter 2 times; With hydrolysate of soybean protein liquid with the flow velocity of the 10 times of column volume/h H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-Millipore filtration by 0.22 μ m is removed particulate; Use ultralow protein adsorption film then, red-tape operati pressure 0.20Mpa, 30 ℃ of temperature, tangential flow technology ultrafiltration 2 hours is adopted in pH=6～6.5; Selection is held back and is limited to 20kD, hold back down and be limited to the 1kD ultra-filtration membrane, obtain the active protein concentrated solution of 20kD～1kD after ultrafiltration, ultrafiltration obtains 20kD～10kD, 10kD～5kD, 5kD～3kD, 3kD～1kD respectively and less than the active soybean polypeptide mixed concentrated liquid of the different fractions of 1kD successively; The soybean polypeptide concentrated solution of the different fractions that obtains is carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain the finished product powder soybean polypeptide of different fractions.
Embodiment two
After 0.2 kilogram of the polynary compound protease that extracts soybean waste residue double centner behind the soybean isoflavones, deionized water double centner, formed by equal mixed by microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase stirred, at pH=5,60 ℃ of following enzyme digestion reactions of temperature 24 hours; The pH value of re-adjustment enzymolysis solution is 4, removes unhydrolysed protein precipitation, obtains soybean polypeptide mixed solution crude product.
The 0.5% times of gac that adds the polypeptide quality in soybean polypeptide mixed solution crude product stirred 10 minutes under 40 ℃, pH=4.0, filtered 2 times; The filtrate H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-, obtain soybean polypeptide mixed solution elaboration.
Soybean polypeptide mixed solution elaboration is removed particulate by the millipore filtration of 0.22 μ m; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the soybean polypeptide mixed solution is carried out classification and concentrate, the red-tape operati temperature is at 20 ℃, pressure 0.25Mpa, pH=7,2 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, and ultrafiltration obtains 20kD～10kD, 10kD～5kD, 5kD～3kD, 3kD～1kD respectively and less than the active soybean polypeptide concentrated solution of the different fractions of 1kD successively.
The soybean polypeptide concentrated solution of each fraction is carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying obtains the powder soybean polypeptide of various fractions.
Embodiment three
With the soybean waste residue that extracts behind the soybean isoflavones, 90 ℃ of heating 5 minutes down, cooling is immediately cleaned, processing such as defibrination separation, fine grinding, filtration; Dregs of beans slag double centner homogenate with pretreated mixes for 300 kilograms with deionized water, joins in the biochemical reactor; Regulate pH=7 with alkali, be warming up to 60 ℃, insulated and stirred 10 minutes; 1 kilogram of the polynary compound protease that adding is formed by equal mixed by microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase in biochemical reactor, after stirring, enzyme digestion reaction 5 hours; PH value by the adjusting protein enzymatic hydrolyzate is 4.5, removes unhydrolysed protein precipitation; The 1.5% times of gac that adds polypeptide quality in the protein enzymatic hydrolyzate stirred filter 23 30 minutes under 30 ℃, pH=2.0; Filtrate is with the flow velocity of the 10 times of column volume/h H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-Again protein enzymatic hydrolyzate is suitably diluted, remove particulate by the millipore filtration of 0.22 μ m; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the polypeptide behind the enzymolysis is carried out classification and concentrate, the red-tape operati temperature is at 40 ℃, pressure 0.1Mpa, pH=5,5 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, and ultrafiltration obtains 20kD～10kD, 10kD～5kD, 5kD～3kD, 3kD～1kD respectively and less than the active soybean polypeptide concentrated solution of the different fractions of 1kD successively; The soybean polypeptide concentrated solution of each fraction is carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying obtains the powder soybean polypeptide of various fractions.
Embodiment four
With the soybean waste residue that extracts behind the soybean isoflavones, 90 ℃ of heating 20 minutes down, cooling is immediately cleaned, processing such as defibrination separation, fine grinding, filtration; Dregs of beans slag double centner homogenate with pretreated mixes for 300 kilograms with deionized water, joins in the biochemical reactor; With acid for adjusting pH 5, be warming up to 30 ℃, insulated and stirred 30 minutes; 0.5 kilogram of the polynary compound protease that adding is formed by equal mixed by isolated microbial protease in the tempeh, stomach en-, papoid, bromeline, 1398 neutral proteinase is in biochemical reactor, after stirring, enzyme digestion reaction 12 hours; PH value by the adjusting protein enzymatic hydrolyzate is 4, removes unhydrolysed protein precipitation; The 1% times of gac that adds polypeptide quality in the protein enzymatic hydrolyzate stirred filter 23 20 minutes under 30 ℃, pH=3.0; With protein enzymatic hydrolyzate with the flow velocity of the 10 times of column volume/h H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-Again protein enzymatic hydrolyzate is suitably diluted, remove particulate by the millipore filtration of 0.22 μ m; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the polypeptide behind the enzymolysis is carried out classification and concentrate, the red-tape operati temperature is at 30 ℃, pressure 0.2Mpa, pH=5,3 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, and ultrafiltration obtains 20kD～10kD, 10kD～5kD, 5kD～3kD, 3kD～1kD respectively and less than the active soybean polypeptide concentrated solution of the different fractions of 1kD successively; The soybean polypeptide concentrated solution of each fraction is carried out the ultrahigh-temperature instantaneous sterilization of 5s respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying obtains the powder soybean polypeptide of various fractions.
Embodiment five
With the soybean waste residue that extracts behind the soybean isoflavones, 90 ℃ of heating 10 minutes down, cooling is immediately cleaned, processing such as defibrination separation, fine grinding, filtration; Dregs of beans slag double centner homogenate with pretreated mixes for 200 kilograms with deionized water, joins in the biochemical reactor; With acid for adjusting pH 6, be warming up to 50 ℃, insulated and stirred 20 minutes; 0.4 kilogram of the polynary compound protease that adding is formed by equal mixed by isolated microbial protease in the tempeh, stomach en-, papoid, bromeline, 1398 neutral proteinase is in biochemical reactor, after stirring, enzyme digestion reaction 10 hours; The pH value of regulating protein enzymatic hydrolyzate is 4, removes unhydrolysed protein precipitation, obtains soybean polypeptide mixed solution crude product; Soybean polypeptide mixed solution crude product is carried out the ultrahigh-temperature instantaneous sterilization of 5s under 135 ℃ of temperature, vacuum concentration then, last spraying drying obtains crude product powder soybean polypeptide.
Embodiment six
In the soybean polypeptide mixed solution crude product of embodiment five preparation, add 1% times of gac of polypeptide quality, under 30 ℃, pH=3.0, stirred 20 minutes, filter 2 times; The filtrate H that flows through respectively
+Type Zeo-karb and OH
-Type anionite-exchange resin removes Na
+And Cl
-, obtain soybean polypeptide mixed solution elaboration; Soybean polypeptide mixed solution elaboration is carried out the ultrahigh-temperature instantaneous sterilization of 5s under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain elaboration powder soybean polypeptide.
Claims (10)
- One kind from the soybean waste residue preparation polypeptide technology, it is characterized in that after extracting 0.2～1.0 part of 100～300 parts of 100 parts in soybean waste residue, deionized waters, polynary compound protease behind the soybean isoflavones and stirring, in pH=5～7,30～60 ℃ of following enzyme digestion reactions of temperature 5～24 hours; The pH value of re-adjustment enzymolysis solution is 4.0～4.5, removes unhydrolysed protein precipitation, obtains soybean polypeptide mixed solution crude product.
- 2. the technology that from the soybean waste residue, prepares polypeptide according to claim 1, it is characterized in that in soybean polypeptide mixed solution crude product, adding 0.5%～1.5% times of gac of polypeptide quality, time stirred 10～30 minutes at 30～40 ℃, pH=2.0～4.0, filter 2～3 times; The filtrate H that flows through respectively +Type Zeo-karb and OH -Type anionite-exchange resin removes Na +And Cl -, obtain soybean polypeptide mixed solution elaboration.
- 3. the technology for preparing polypeptide from the soybean waste residue according to claim 2 is characterized in that the millipore filtration removal particulate of soybean polypeptide mixed solution elaboration by 0.22 μ m; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the soybean polypeptide mixed solution is carried out classification and concentrate, the red-tape operati temperature is at 20～40 ℃, pressure 0.1～0.25Mpa, pH=5～7,2～5 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, obtains the soybean polypeptide concentrated solution of different fractions after ultrafiltration.
- 4. according to claim 1,2, the 3 described technology that from the soybean waste residue, prepare polypeptide, it is characterized in that the soybean polypeptide concentrated solution of soybean polypeptide mixed solution crude product or soybean polypeptide mixed solution elaboration or each fraction carried out respectively 5 seconds ultrahigh-temperature instantaneous sterilization under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain the powder soybean polypeptide of crude product powder soybean polypeptide or elaboration powder soybean polypeptide or various fractions.
- 5. the technology that from the soybean waste residue, prepares polypeptide according to claim 1, the soybean waste residue that it is characterized in that extracting behind the soybean isoflavones is used further to enzyme digestion reaction after pre-treatment, pretreatment technology is: will extract the soybean waste residue behind the soybean isoflavones, heated 5～20 minutes down at 90 ℃, cooling is immediately cleaned, defibrination, coarse filtration.
- 6. according to claim 1, the 5 described technology that from the soybean waste residue, prepare polypeptide, it is characterized in that 100～300 parts of 100 parts in soybean waste residue, deionized waters extracting behind the soybean isoflavones are regulated pH=5～7 with acid or alkali, be warming up to 30～60 ℃, after the insulated and stirred 10～30 minutes, add polynary compound protease again and carry out enzyme digestion reaction.
- According to claim 1,2,3 described from the soybean waste residue technology of preparation polypeptide, it is characterized in that the soybean waste residue that will extract behind the soybean isoflavones, 90 ℃ of heating 10 minutes down, cooling is immediately cleaned, defibrination, coarse filtration handle; Through above-mentioned pretreated 100 parts of soybean waste residues and 100～300 parts of deionized waters, add in the biochemical reactor; Regulate pH=5～7 with acid or alkali, be warming up to 30～50 ℃, insulated and stirred 10～30 minutes; Add 0.2～1.0 part of polynary compound protease in biochemical reactor, after stirring, enzyme digestion reaction 5～24 hours; PH value by the adjusting protein enzymatic hydrolyzate is 4.0～4.5, removes unhydrolysed protein precipitation; Add 0.5%～1.5% times of gac of polypeptide quality in the hydrolysate of soybean protein liquid, time stirred 10～30 minutes, filter 2～3 times at 30～40 ℃, pH=2.0～4.0; With the hydrolysate of soybean protein liquid H that flows through respectively +Type Zeo-karb and OH -Type anionite-exchange resin removes Na +And Cl -Remove particulate by the millipore filtration of 0.22 μ m again; Adopt ultralow protein adsorption film and cross-flow ultrafiltration technology, the polypeptide behind the enzymolysis is carried out classification and concentrate, the red-tape operati temperature is at 20～40 ℃, pressure 0.1～0.25Mpa, pH=5～7,2～5 hours ultrafiltration time; Selection is held back and is limited to 20kD, holds back down to be limited to the 1kD ultra-filtration membrane, obtains the soybean polypeptide concentrated solution of different fractions after ultrafiltration; The soybean polypeptide concentrated solution of the different fractions that obtains is carried out 5 seconds ultrahigh-temperature instantaneous sterilization respectively under 135 ℃ of temperature, vacuum concentration then, last spraying drying can obtain the finished product powder soybean polypeptide of different fractions.
- 8. the technology for preparing polypeptide from the soybean waste residue according to claim 1 is characterized in that polynary compound protease is that microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase are by equal mixed.
- 9. the technology for preparing polypeptide from the soybean waste residue according to claim 7 is characterized in that polynary compound protease is that microbial protease, stomach en-, papoid, bromeline, 1398 neutral proteinase are by equal mixed.
- 10. according to Claim 8,9 described from the soybean waste residue preparation polypeptide technology, it is characterized in that microbial protease is an isolated microbial protease from tempeh.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824455B (en) * | 2009-03-06 | 2013-07-17 | 香港百特有限公司 | Soy protein oligopeptide, and preparation method and use thereof |
| CN112645741A (en) * | 2020-12-14 | 2021-04-13 | 剑胜作物科技(江苏盐城)有限责任公司 | Preparation method for environment-friendly agricultural lecithin and amino acid co-production |
| CN112931881A (en) * | 2021-03-10 | 2021-06-11 | 山东泰好生物科技有限公司 | Production method of green bean NMN small peptide powder and SOD soft capsule thereof |
| CN114525314A (en) * | 2022-03-18 | 2022-05-24 | 广西七虹灯农业科技有限公司 | Process for producing amino acid by enzymolysis method |
-
2003
- 2003-04-01 CN CNA031175864A patent/CN1534098A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824455B (en) * | 2009-03-06 | 2013-07-17 | 香港百特有限公司 | Soy protein oligopeptide, and preparation method and use thereof |
| CN112645741A (en) * | 2020-12-14 | 2021-04-13 | 剑胜作物科技(江苏盐城)有限责任公司 | Preparation method for environment-friendly agricultural lecithin and amino acid co-production |
| CN112931881A (en) * | 2021-03-10 | 2021-06-11 | 山东泰好生物科技有限公司 | Production method of green bean NMN small peptide powder and SOD soft capsule thereof |
| CN114525314A (en) * | 2022-03-18 | 2022-05-24 | 广西七虹灯农业科技有限公司 | Process for producing amino acid by enzymolysis method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |