CN115530372B - Seaweed extract with lipid-lowering function, preparation method thereof and seaweed extract composition - Google Patents
Seaweed extract with lipid-lowering function, preparation method thereof and seaweed extract composition Download PDFInfo
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- CN115530372B CN115530372B CN202211224706.7A CN202211224706A CN115530372B CN 115530372 B CN115530372 B CN 115530372B CN 202211224706 A CN202211224706 A CN 202211224706A CN 115530372 B CN115530372 B CN 115530372B
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- enzymolysis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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Abstract
The invention belongs to the technical field of health-care foods, and particularly relates to a seaweed extract with a lipid-reducing function, a preparation method thereof and a seaweed extract composition. The invention provides an alga extract, which has an average relative molecular weight of 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage of more than or equal to 1.5 wt%. The fucoxanthin in the seaweed extract provided by the invention belongs to natural carotenoid, is fat-soluble pigment, and has obvious fat-reducing effect by inhibiting the formation of fat cells; meanwhile, the average relative molecular weight of the seaweed extract provided by the invention is 1000-30000 Da, and the seaweed extract is easy to be absorbed by human bodies. The seaweed extract composition provided by the invention has the effects of effectively increasing fat metabolism, inhibiting fat cell generation, reducing body fat rate even when a human body is static, and reducing weight.
Description
Technical Field
The invention belongs to the technical field of health-care foods, and particularly relates to a seaweed extract with a lipid-reducing function, a preparation method thereof and a seaweed extract composition.
Background
In the current society, the life rhythm is gradually accelerated, the modern artificial pressure is increasingly increased, the working and learning time is overlong, the exercise time is reduced, the exercise time is prolonged, the obesity, constipation, weight and body fat are increased due to the fact that the exercise time is prolonged and the exercise time is prolonged. In addition, modern people are busy in work and always eat fast food and take out, the dietary structure is unreasonable, the nutrition intake is excessive, the nutrition is unbalanced, the nutrition is rich in oil and salt, and the weight and body fat are increased due to high heat. Obesity is mainly due to improper natural survival after excessive intake of food, hypoexercise, etc. Obesity not only can bring about changes of body forms, but also can cause increases of incidence rate of chronic diseases such as hypertension, hyperlipidemia, diabetes, coronary heart disease, malignant tumor and the like, and nowadays obesity is an invisible "killer" in our lives.
Weight loss products on the market now generally fall into the following categories: 1. the method has obvious somatosensory effect and high speed, but can be accompanied with abdominal discomfort and distending pain, and can cause damage to intestinal tracts after long-term administration, and the effect of losing weight is not obvious; 2. meal replacement, through meal replacement, reduces energy intake, has bad taste, is easy to hunger, is not easy to adhere to, and can cause rebound; 3. weight-losing medicines have great side effects and damage to the body; 4. exercise and fitness, healthy weight loss is good for the body, but modern people have limited time, and most people cannot adhere to the body for personal reasons and do not want to exercise.
Therefore, the currently marketed weight-reducing products cannot realize effective reduction of body fat when the human body is static, and the weight-reducing effect is poor.
Disclosure of Invention
The invention aims to provide a seaweed extract with a fat reducing function, a preparation method thereof and a seaweed extract composition, and the seaweed extract provided by the invention can inhibit the formation of fat cells and has a remarkable fat reducing effect; the seaweed extract composition provided by the invention can effectively increase fat metabolism, inhibit fat cell generation, and has the effect of reducing body fat rate even when a human body is static, and has remarkable weight-losing effect.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a seaweed extract with a fat reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage of more than or equal to 1.5 wt%.
Preferably, the mass percentage of fucoxanthin in the seaweed extract is 1.5-3 wt%.
The invention provides a preparation method of the seaweed extract with the lipid-lowering function, which comprises the following steps:
mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and carrying out solid-liquid separation to obtain seaweed enzymolysis sediment; the complex hydrolase comprises alginic acid lyase and cellulase;
dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on the leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid;
concentrating the seaweed extract purified solution, and drying to obtain the seaweed extract with the lipid-reducing function.
Preferably, the mass of the compound hydrolase is 0.5-1.0 per mill of the fresh weight of the fresh seaweed.
Preferably, the mass ratio of the alginic acid lyase to the cellulase is (1-3): 1-3; the enzyme activity units of the alginic acid lyase and the cellulase are independently 5-50 ten thousand U/g.
Preferably, the enzymolysis temperature is 35-60 ℃; the heat preservation time of the enzymolysis is 1-6 h; the pH value of the enzymolysis is 5-9.
Preferably, before mixing, the method further comprises preparing the fresh seaweed into seaweed aqueous slurry, and crushing the seaweed aqueous slurry to obtain seaweed particle slurry; the granularity of the seaweed particles in the seaweed particle slurry is 10-30 mu m.
Preferably, the crushing is colloid mill crushing, and the crushing power of the colloid mill is 37-45W; the grinding speed of the colloid mill is 2000-3000 r/min.
The invention provides a seaweed extract composition, which comprises the following components in parts by mass: 1 to 35 parts of seaweed extract, 1 to 30 parts of mulberry leaf extract, 1 to 20 parts of green tea, 1 to 20 parts of coffee, 1 to 20 parts of green coffee, 1 to 10 parts of L-carnitine and 1 to 15 parts of Opuntia ficus-indica; the seaweed extract is the seaweed extract with the lipid-reducing function in the technical scheme or the seaweed extract with the lipid-reducing function prepared by the preparation method in the technical scheme.
Preferably, the food additive further comprises auxiliary materials, wherein the auxiliary materials comprise one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food essence, xanthan gum, citric acid, povidone K30 and gelatin.
The invention provides a seaweed extract with a fat reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage of more than or equal to 1.5 wt%. The mass percentage of fucoxanthin in the seaweed extract provided by the invention is more than or equal to 1.5wt%, and the fucoxanthin belongs to natural carotenoid, is a fat-soluble pigment, and has remarkable fat reducing effect by inhibiting the formation of fat cells; meanwhile, the average relative molecular weight of the seaweed extract provided by the invention is 1000-30000 Da, and the seaweed extract is easy to be absorbed by human bodies.
The invention provides a preparation method of the seaweed extract with the lipid-lowering function, which comprises the following steps: mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and carrying out solid-liquid separation to obtain seaweed enzymolysis sediment; the complex hydrolase comprises alginic acid lyase and cellulase; dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and carrying out anion-cation exchange resin chromatography on the leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid; concentrating the seaweed extract purified solution, and drying to obtain the seaweed extract with the lipid-reducing function. According to the preparation method provided by the invention, the composite hydrolase comprising the alginic acid lyase and the cellulase is adopted to carry out enzymolysis reaction on the seaweed, so that the molecular weight of alginic acid in the seaweed is effectively reduced, fucoxanthin in the seaweed is more easily separated from the seaweed, and then the seaweed is further leached by adopting the alcohol solvent; after the alcohol solvent is further enriched in fucoxanthin, the fucoxanthin is purified by anion-cation exchange resin chromatography, and the obtained seaweed extract has high fucoxanthin content and moderate molecular weight and is easy to be absorbed by human body.
The invention provides a seaweed extract composition, which comprises the following components in parts by mass: 1 to 35 parts of seaweed extract, 1 to 30 parts of mulberry leaf extract, 1 to 20 parts of green tea, 1 to 20 parts of coffee, 1 to 20 parts of green coffee, 1 to 10 parts of L-carnitine and 1 to 15 parts of Opuntia ficus-indica; the seaweed extract is the seaweed extract with the lipid-reducing function in the technical scheme or the seaweed extract with the lipid-reducing function prepared by the preparation method in the technical scheme. In the invention, the mulberry leaf extract has the effects of reducing blood sugar and blood fat and inhibiting adipogenesis; tea polyphenols contained in green tea can inhibit fat absorption and in vivo fat generation; caffeine in coffee has the effect of increasing the concentration of fatty acids in blood, and once the concentration of fatty acids in blood increases, fatty acids are absorbed by muscles and consumed in a manner of converting body energy, so that fat accumulated in the body is decomposed; the chlorogenic acid is contained in the green coffee, can obviously inhibit the activities of fatty acid synthase, HMG-CoA reductase and fat coenzyme A, ACT in a fat metabolism pathway, and improves the expression of a fatty acid beta-oxidation and peroxisome proliferator-activated receptor alpha, so that the lipid metabolism is regulated, and the activity of inhibiting fat and promoting fat decomposition is obvious; l-carnitine is a key substance in the fat metabolism process, can promote fatty acid to enter mitochondria for oxidative decomposition, is a carrier for transporting fatty acid, and can increase the oxidation rate of fat and reduce glycogen consumption; the seaweed extract composition provided by the invention is prepared by compounding the seaweed extract with the mulberry leaf extract, green tea, coffee, green coffee, L-carnitine and Opuntia ficus-indica, and has the remarkable effects of effectively increasing fat metabolism, inhibiting lipogenesis and reducing body fat rate even when a human body is static in part by mass.
The seaweed extract composition provided by the invention can be prepared into various dosage forms, is stable in product and is easy for industrial production.
Drawings
FIG. 1 is a liquid chromatogram of seaweed extract with lipid-lowering function prepared in example 1 of the present invention.
Detailed Description
The invention provides a seaweed extract with a fat reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage of more than or equal to 1.5 wt%.
In the present invention, all preparation materials/components are commercially available products well known to those skilled in the art unless specified otherwise.
In the present invention, the mass percentage of fucoxanthin in the seaweed extract is preferably 1.5 to 3wt%.
The seaweed extract provided by the invention has the effects of burning fat and promoting fat metabolism, and has obvious effect of reducing weight.
The invention provides a preparation method of the seaweed extract with the lipid-lowering function, which comprises the following steps:
mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and carrying out solid-liquid separation to obtain seaweed enzymolysis sediment; the complex hydrolase comprises alginic acid lyase and cellulase;
dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on the leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid with the lipid-reducing function;
concentrating the seaweed extract purified solution, and drying to obtain the seaweed extract with the lipid-reducing function.
Fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase are mixed for enzymolysis, and seaweed enzymolysis precipitate is obtained after solid-liquid separation (hereinafter referred to as first solid-liquid separation); the complex hydrolase comprises alginic acid lyase and cellulase.
In the present invention, the fresh seaweed is particularly preferably fresh kelp and/or fresh undaria pinnatifida.
In the present invention, the mass percentage of the solids of the fresh seaweed is preferably not less than 15%, more preferably not less than 20%.
In a specific embodiment of the invention, the fresh seaweed is preferably purchased from Qingdao, shandong.
In the present invention, the fresh seaweed does not exhibit a large area yellowing or dehydration phenomenon.
The source of the fresh seaweed is not particularly limited, and the seaweed can be obtained by adopting a commercially available product or self-preparation which are conventional in the field.
In the invention, before mixing, the method further comprises the steps of preparing the fresh seaweed into seaweed water slurry, and crushing the seaweed water slurry to obtain seaweed particle slurry; the granularity of the seaweed particles in the seaweed particle slurry is 10-30 mu m.
In the present invention, the present invention preferably further comprises crushing the fresh seaweed before the crushing. Obtaining seaweed slices; preparing the seaweed pieces into seaweed water slurry, and crushing the seaweed water slurry. In the invention, the particle size of the seaweed pieces is less than or equal to 20 meshes. The invention has no special requirements for the specific implementation process of the crushing.
In the present invention, the mass percentage of the seaweed or seaweed pieces in the aqueous slurry of fresh seaweed or the aqueous slurry of seaweed pieces is preferably 10% to 20%, more preferably 15% to 17%.
In the present invention, the pulverization is colloid mill pulverization, and the power of the colloid mill pulverization is preferably 37 to 45W, more preferably 45W.
In the invention, the grinding speed of the colloid mill is preferably 2000-3000 r/min.
In the present invention, the particle size of seaweed particles in the seaweed particle slurry is preferably 10 to 30. Mu.m, more preferably 20. Mu.m.
In the present invention, the water-soluble inorganic calcium salt is particularly preferably calcium chloride.
In the present invention, the mixing of fresh seaweed, water-soluble inorganic calcium salt and complex hydrolase preferably comprises the steps of: ultrasonically mixing the fresh seaweed, water and water-soluble inorganic calcium salt to obtain a feed liquid to be subjected to enzymolysis; and mixing the feed liquid to be subjected to enzymolysis with the composite hydrolase. In the present invention, the time of the ultrasonic wave is preferably 0.5 to 3 hours.
In the invention, the mass of the water-soluble inorganic calcium salt is 1-2 of the fresh weight of the fresh seaweed during enzymolysis.
In the invention, the mass of the compound hydrolase is 0.5 to 1.0 per mill of the fresh weight of the fresh seaweed, and more preferably 0.6 to 0.8 per mill.
In the present invention, the mass ratio of the alginic acid lyase to the cellulase is preferably (1-3): 1-3, more preferably (1-2): 1-2.
In the present invention, the enzyme activity unit of the alginic acid lyase is preferably 5 to 50 ten thousand U/g, more preferably 20 ten thousand U/g or 50 ten thousand U/g.
In the present invention, the alginic acid lyase is purchased from Jiangxi Baiying biotechnology Co.
In the present invention, the enzyme activity unit of the cellulase is preferably 5 to 50 ten thousand U/g, more preferably 20 ten thousand U/g or 50 ten thousand U/g.
In the present invention, the cellulase is purchased from novelian (china) biotechnology limited.
In the present invention, the temperature of the enzymolysis is preferably 35 to 60 ℃, more preferably 40 to 50 ℃.
In the present invention, the incubation time for the enzymolysis is preferably 1 to 6 hours, more preferably 2 to 3 hours.
In the present invention, the pH of the enzymatic hydrolysis is preferably 5 to 9, more preferably 6 to 8.
In the present invention, the enzymolysis reaction liquid is obtained after the enzymolysis reaction, and the first solid-liquid separation is preferably performed after the enzymolysis reaction liquid is subjected to enzyme deactivation treatment. In the present invention, the specific embodiment of the enzyme deactivation treatment is preferably: heating the enzymolysis reaction liquid to the enzyme deactivation temperature for heat preservation and enzyme deactivation; in the invention, the enzyme deactivation temperature is preferably 70-90 ℃, and the enzyme deactivation heat preservation time is preferably 30-60 min. In the present invention, the enzyme deactivation is performed by denaturing and deactivating various enzymes in the complex hydrolase in the enzymatic reaction solution, thereby terminating the enzymatic reaction.
In the present invention, the specific embodiment of the first solid-liquid separation is preferably filtration. In the present invention, the filtration is preferably performed using a filter screen having a mesh size of preferably 20 to 40 mesh, more preferably 20 mesh or 40 mesh. In the invention, the seaweed enzymolysis sediment is obtained by the first solid-liquid separation.
After the seaweed enzymolysis sediment is obtained, the seaweed enzymolysis sediment is dispersed in an alcohol solvent for leaching, and the leaching filtrate after solid-liquid separation (hereinafter referred to as second solid-liquid separation) is sequentially subjected to anion exchange resin chromatography and cation exchange resin chromatography to obtain seaweed extract purified liquid.
In the present invention, the alcohol solvent is particularly preferably ethanol or an ethanol-water mixed solvent.
In the present invention, the ethanol-water mixed solvent preferably has a volume percentage of ethanol of 95 to 100% and is not equal to 100%.
In the invention, the mass ratio of the seaweed enzymolysis sediment to the alcohol solvent is 1:5.
In the present invention, the time of the leaching is preferably 1h.
In the present invention, the leaching is preferably performed under stirring.
In the present invention, the second solid-liquid separation is preferably performed by filtration. The invention is not particularly limited to the specific embodiments of the concerns. In the present invention, the filtration is preferably performed using a filter screen having a mesh size of preferably 20 to 40 mesh, more preferably 20 mesh or 40 mesh.
In the present invention, the anion exchange resin chromatography embodiment is preferably: subjecting the leaching filtrate to chromatography by passing through the anion exchange resin column.
In the present invention, the cation exchange resin chromatography is preferably performed as follows: and (3) carrying out chromatography on the initial chromatographic liquid after the anion exchange resin chromatography by a cation exchange resin column.
In the present invention, the cation exchange resin is used for chromatography to obtain a chromatography liquid, and the chromatography liquid is preferably subjected to post-treatment to obtain the seaweed extract purification liquid. In the present invention, the post-treatment preferably includes: and filtering the chromatographic liquid by a filter column and ultrafiltering by an ultrafiltration column in sequence. In the present invention, the pore size of the filter column is preferably 0.5 to 1 μm; the specification of the filter element of the ultrafiltration column is preferably 0.02 mu m.
After the seaweed extract purified solution is obtained, concentrating the seaweed extract purified solution and drying to obtain the seaweed extract with the lipid-reducing function.
In the present invention, the concentration is preferably concentration under reduced pressure.
In the present invention, the vacuum degree of the reduced pressure concentration is preferably 0.8 to 1.0Mpa.
In the present invention, the temperature of the reduced pressure concentration is preferably 30 to 40 ℃.
In the present invention, the concentration gives a concentrated solution, and the mass percentage of the concentrated solution is preferably 15 to 30%, more preferably 20 to 25%. The concentrate is preferably dried in the present invention.
In the present invention, the drying is particularly preferably freeze-drying.
In the present invention, the temperature of the freeze-drying is preferably-20 to-50 ℃.
In the present invention, the degree of vacuum of the freeze-drying is preferably-0.07 to-0.09 Mpa.
The invention provides a seaweed extract composition, which comprises the following components in parts by mass: 1 to 35 parts of seaweed extract, 1 to 30 parts of mulberry leaf extract, 1 to 20 parts of green tea, 1 to 20 parts of coffee, 1 to 20 parts of green coffee, 1 to 10 parts of L-carnitine and 1 to 15 parts of Opuntia ficus-indica; the seaweed extract is the seaweed extract with the lipid-reducing function in the technical scheme or the seaweed extract with the lipid-reducing function prepared by the preparation method in the technical scheme.
The seaweed extract composition provided by the invention comprises 1-35 parts by mass of the seaweed extract composition, preferably 10-30 parts by mass, and more preferably 13 parts by mass.
In the present invention, the particle size of the seaweed extract group is preferably 0.25mm or less, more preferably 0.18mm
The seaweed extract composition provided by the invention comprises 1-30 parts of mulberry leaf extract, preferably 5-20 parts, more preferably 10 parts, based on the mass parts of the seaweed extract.
In the present invention, the purity of the mulberry leaf extract is preferably 95 to 99%.
In the present invention, the particle size of the mulberry leaf extract is preferably not more than 0.25mm, more preferably 0.18mm
The source of the mulberry leaf extract is not particularly limited, and the mulberry leaf extract is obtained by adopting products conventionally sold in the art, for example, food grade and purity of 98% of Dissman (China) Limited.
The seaweed extract composition provided by the invention comprises 1-20 parts of green tea, preferably 5-20 parts, and more preferably 7 parts, based on the mass parts of the seaweed extract.
In the present invention, the green tea is preferably a green tea powder, and the particle size of the green tea powder is preferably 0.25mm or less, more preferably 0.18mm or less.
In the present invention, the purity of the green tea is preferably 95 to 99%.
The source of the green tea is not particularly limited, and the green tea can be obtained by adopting products conventionally sold in the field, for example, food grade and purity of 98% of Quzhou Co., ltd.
The seaweed extract composition provided by the invention comprises 1-20 parts of coffee, preferably 5-20 parts, more preferably 7 parts, based on the mass parts of the seaweed extract.
In the present invention, the coffee is preferably a coffee powder, and the particle size of the coffee powder is preferably 0.25mm or less, more preferably 0.18mm.
In the present invention, the purity of the coffee is preferably 90 to 99%; the source of the coffee is not particularly limited in the present invention, and conventional commercial products in the art, for example, dehong rear grain coffee limited, food grade, purity 95%, may be used.
The seaweed extract composition provided by the invention comprises 1-20 parts of green coffee, preferably 5-20 parts, more preferably 7 parts, based on the mass parts of the seaweed extract.
In the present invention, the green coffee is particularly preferably a green coffee powder, and the particle size of the green coffee powder is preferably 0.25mm or less, more preferably 0.18mm.
In the present invention, the purity of the green coffee is preferably 50 to 99%; the source of the green coffee is not particularly limited, and the green coffee can be obtained by adopting products which are conventionally sold in the field, for example, guangzhou energy and beauty biotechnology company, and the purity is 90%.
The seaweed extract composition provided by the invention comprises 1-10 parts of L-carnitine, preferably 1-5 parts, and more preferably 3 parts, based on the mass parts of the seaweed extract.
In the present invention, the L-carnitine is preferably L-carnitine powder, and the particle size of the L-carnitine powder is preferably not more than 0.25mm, more preferably 0.18mm.
In the invention, the purity of the L-carnitine is preferably 95-99%; the source of the L-carnitine is not particularly limited, and the L-carnitine powder can be obtained by adopting products which are conventionally sold in the field, such as Liaoning scientific and technological company, inc.
The seaweed extract composition provided by the invention comprises 1-15 parts of Opuntia ficus-indica, preferably 1-10 parts, more preferably 3 parts, based on the mass parts of the seaweed extract.
In the invention, the Opuntia ficus-indica is particularly preferably an Opuntia ficus-indica powder, and the particle size of the Opuntia ficus-indica powder is preferably less than or equal to 0.25mm, more preferably 0.18mm.
In the invention, the purity of the Opuntia ficus-indica is preferably 50-99%; the source of the Opuntia ficus-indica is not particularly limited, and the conventional commercial products in the field, such as the powder purity of the Opuntia ficus-indica 70% of Fengnoto biotechnology Co., ltd, can be adopted.
In the seaweed extract composition provided by the invention, a synergistic effect exists between the seaweed extract group and coffee, green coffee, mulberry leaf extract, green tea, L-carnitine and Opuntia ficus-indica. Fucoxanthin in seaweed extract belongs to natural carotenoid, has obvious fat burning effect, and can inhibit lipogenesis and promote fat metabolism when a human body is static. The method comprises the following steps: the fucose Huang Zhineng can regulate the expression of mitochondrial uncoupling protein (UCP 1) in WAT (white fat) in human body, and the effect can reduce WAT formation and achieve lipid-reducing effect. Caffeine in coffee has the effect of increasing the concentration of fatty acids in the blood, which are absorbed by the muscles and consumed in a way that converts body energy once the concentration of fatty acids in the blood increases. That is, the fat accumulated in the body is decomposed. The green coffee also has obvious functions of inhibiting fat and promoting lipolysis, and chlorogenic acid contained in the green coffee can obviously inhibit the activities of fatty acid synthase, HMG-CoA reductase and fat coenzyme A, ACT in fat metabolism pathways, and improve the expression of fatty acid beta-oxidation and peroxisome proliferator-activated receptor alpha, so that the lipid metabolism is regulated. The tea polyphenols of green tea can inhibit fat absorption and in vivo fat generation. L-carnitine is a key substance in the fat metabolism process, can promote fatty acid to enter mitochondria for oxidative decomposition, and is a carrier for transporting fatty acid.
In the present invention, the main mechanism of the seaweed extract composition is that coffee, green coffee, mulberry leaf extract, green tea powder, and L-carnitine provide various components for promoting fat burning, improving fat metabolism, and simultaneously, the fat burning is carried out, fucoxanthin can reduce the generation of WAT (white fat) by regulating the expression of mitochondrial uncoupling protein (UCP 1) in WAT (white fat), promote the generation of BAT (brown fat), and the fucoxanthin and the above five fat burning components act synergistically, so that the occupation ratio of WAT is reduced, and the occupation ratio of BAT is increased, thereby achieving the effects of fat burning and fat reduction.
The seaweed extract composition provided by the invention preferably further comprises auxiliary materials.
In the present invention, the auxiliary materials preferably include one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food essence, xanthan gum, citric acid, povidone K30, and gelatin.
The type and amount of the auxiliary materials are not particularly limited in the present invention, and are preferably selected and determined according to the specific formulation of the seaweed extract composition.
In the present invention, the formulation of the seaweed extract composition is preferably a tablet, granule, capsule, powder or oral liquid.
In the present invention, the mass of the auxiliary material is preferably 10 to 80% of the mass of the seaweed extract composition.
In the present invention, when the formulation of the seaweed extract composition is preferably a tablet, the auxiliary materials are particularly preferably sorbitol, microcrystalline cellulose, food essence and magnesium stearate.
In the present invention, when the formulation of the seaweed extract composition is preferably a tablet, the seaweed extract composition tablet comprises, by mass, 13% of the seaweed extract, 10% of the mulberry leaf extract, 7% of green tea powder, 7% of coffee powder, 7% of green coffee powder, 3% of L-carnitine powder, 3% of Opuntia ficus-indica powder, 40% of sorbitol, 8% of microcrystalline cellulose, 0.7% of food essence and 1.3% of magnesium stearate.
In the present invention, when the formulation of the seaweed extract composition is preferably a powder, the auxiliary materials are particularly preferably sorbitol, microcrystalline cellulose, food essence and magnesium stearate.
In the invention, when the formulation of the seaweed extract composition is preferably powder, the seaweed extract composition tablet comprises 13% of seaweed extract, 10% of mulberry leaf extract, 7% of green tea powder, 7% of coffee powder, 7% of green coffee powder, 3% of L-carnitine powder, 3% of Opuntia ficus-indica powder, 40% of sorbitol, 8% of microcrystalline cellulose, 0.7% of food essence and 1.3% of magnesium stearate according to the above technical scheme.
In the invention, when the formulation of the seaweed extract composition is preferably an oral liquid, the auxiliary materials are particularly preferably water, fruit powder, xanthan gum, sucralose, citric acid and food essence. The fruit powder is particularly preferably apple powder; the food essence is particularly preferably apple essence; the water is preferably purified water.
In the invention, when the formulation of the seaweed extract composition is preferably oral liquid, the seaweed extract composition tablet comprises 2.6% of seaweed extract, 2% of mulberry leaf extract, 1.4% of green tea powder, 1.4% of coffee powder, 1.4% of green coffee powder, 0.6% of L-carnitine powder, 0.6% of pear cactus powder, 5% of apple powder, 0.2% of xanthan gum, 0.1% of sucralose, 0.3% of citric acid, 0.1% of apple essence and 84.3% of water according to the mass percentage.
The invention also provides a preparation method of the seaweed extract composition, which comprises the following steps:
mixing seaweed extract, folium Mori extract, green tea, coffee, green coffee, L-carnitine, and Opuntia Dillenii to obtain the seaweed extract composition.
In the present invention, the above raw materials are preferably separately screened through a 60-80 mesh sieve to collect undersize components to obtain screened raw materials, and then the screened raw materials are mixed to obtain the seaweed extract composition.
The specific operation of the sieving is not particularly limited in the present invention, and a conventional sieving operation in the art may be adopted.
After the sieving raw materials are obtained, mixing the sieving raw materials to obtain a seaweed extract composition; the mixing is preferably carried out in a three-dimensional mixer, the rotational speed of the mixing is preferably 10 to 20rpm, and the mixing time is preferably 20 to 40min.
In the present invention, when the formulation of the seaweed extract composition is preferably a tablet, the present invention preferably further comprises tabletting the mixed material obtained by the mixing after the mixing, and the tabletting is preferably carried out by a tablet press.
In the present invention, when the formulation of the seaweed extract composition is preferably an oral liquid, the mixing is preferably performed in ingredients, and the mixing is particularly preferably: mixing water with the screened raw materials and related auxiliary materials to obtain compound feed liquid; filtering the compound liquid through a filter element type filter; obtaining a filtered feed liquid; sterilizing the filtered feed liquid at high temperature by UHT; obtaining a sterilizing feed liquid; and (3) filling the sterilized feed liquid into the oral liquid by an automatic filling machine.
The technical solutions provided by the present invention are described in detail below with reference to the drawings and examples for further illustrating the present invention, but they should not be construed as limiting the scope of the present invention.
Example 1
Cleaning fresh kelp (purchased from Shandong Qingdao market, wherein the solid content of fresh kelp is more than or equal to 20%), and pulverizing to less than or equal to 20 meshes to obtain small pieces; crushing the water slurry of the crushed seaweed sheets (the seaweed sheets are 15 percent) by using a colloid mill, wherein the power of the colloid mill treatment is 45W, and the rotating speed is 2500r/min; grinding into slurry of seaweed particles with particle size of 20 μm.
Mixing seaweed granule slurry, water and calcium chloride by ultrasonic method, and mixing with compound hydrolase for enzymolysis, wherein the compound hydrolase is alginic acid lyase (purchased from Jiangxi Baiying biotechnology Co., ltd., 50U /) and cellulase (purchased from NoveXin (China) biotechnology Co., ltd., 50U/g), the mass ratio of alginic acid lyase to cellulase is 2:1, the dosage of compound hydrolase is 0.8 per mill of fresh kelp fresh weight, and the mass of calcium chloride is 1 of fresh kelp fresh weight; the enzymolysis temperature is controlled at 45+/-5 ℃, the enzymolysis time is 2 hours, and the pH value of enzymolysis is 6;
after enzymolysis, heating the enzymolysis reaction liquid to 75 ℃ to inactivate enzyme for 40min, so as to denature and inactivate alginic acid lyase and cellulase in the compound hydrolase, and filtering the enzymolysis reaction liquid after the enzymolysis reaction is terminated by using a 40-mesh filter screen to obtain seaweed enzymolysis precipitate;
adding ethanol into the seaweed enzymolysis sediment, wherein the addition mass of the ethanol is 5 times of the seaweed enzymolysis sediment mass, and stirring for 1 hour for leaching; filtering the leaching solution by a 40-mesh filter screen, sequentially passing the obtained leaching solution through anion exchange resin and cation exchange resin for chromatography, filtering by a filter column (the micropores of the filter column are 0.5-1 mu m), and ultrafiltering by an ultrafiltration column (the specification of the filter element is 0.02 mu m) to obtain seaweed extract purified solution containing fucoxanthin;
concentrating the obtained seaweed extract purified solution to 20 mass percent by adopting a reduced pressure distillation method to obtain concentrated solution;
freeze drying (freeze drying temperature-50deg.C, vacuum degree-0.09 Mpa) the concentrated solution, and freeze drying for 48 hr to obtain seaweed extract.
Detecting the content of the seaweed extract: the seaweed extract obtained in this example was subjected to fucoxanthin content detection by high performance liquid chromatography (SPD-10 AVP model Shimadzu, japan) as shown in FIG. 1, to obtain fucoxanthin content of 3.54%.
Example 2
The preparation process was essentially the same as in example 1, except that: the mass ratio of the alginic acid lyase to the cellulase is 1:2.
Example 3
The preparation process was essentially the same as in example 1, except that: the mass ratio of the alginic acid lyase to the cellulase is 1:1.
Example 4
The preparation process was essentially the same as in example 1, except that: the mass ratio of the alginic acid lyase to the cellulase is 3:1.
Example 5
The preparation process was essentially the same as in example 1, except that: the mass ratio of the alginic acid lyase to the cellulase is 1:3.
Comparative example 1
The preparation process was essentially the same as in example 1, except that: only alginic acid lyase is used for enzymolysis.
Comparative example 2
The preparation process was essentially the same as in example 1, except that: only cellulase is adopted for enzymolysis.
Examples 1 to 5 and comparative examples 1 to 2 are shown in Table 1, together with the enzyme types, the amounts added and the fucoxanthin content of the live seaweed extract.
TABLE 1 content of fucoxanthin obtained after enzymatic hydrolysis of examples 1 to 5 and comparative examples 1 to 2
| Sequence number | Enzymes | Additive amount | Fucoxanthin content% |
| Comparative example 1 | Alginic acid lyase | 0.8‰ | 0.71 |
| Comparative example 2 | Cellulase enzymes | 0.8‰ | 0.63 |
| Example 2 | Alginic acid lyase and cellulase 1:2 | 0.8‰ | 1.91 |
| Example 3 | Alginic acid lyase and cellulase 1:1 | 0.8‰ | 2.21 |
| Example 1 | Alginic acid lyase and cellulase 2:1 | 0.8‰ | 3.54 |
| Example 4 | Alginic acid lyase and cellulase 3:1 | 0.8‰ | 2.31 |
| Example 5 | Alginic acid lyase and cellulase 1:3 | 0.8‰ | 2.19 |
As can be seen from the data in table 1, the fucoxanthin content of the compound hydrolytic enzyme obtained by combining the two enzymes in different proportions is different, mainly because the two enzymes in different combinations have different enzymolysis effects on alginic acid, but are obviously higher than the fucoxanthin content in the product obtained by enzymolysis with one enzyme, and the reason is that: according to the invention, the molecular weight of alginic acid can be reduced by compounding alginic acid lyase and cellulase, so that fucoxanthin in fresh seaweed is more easily removed from the seaweed, and further, the content of fucoxanthin can be increased in the subsequent ethanol extraction, wherein in the embodiment 1, the fucoxanthin in the seaweed extract is the maximum by adopting the enzymolysis of the alginic acid lyase and the cellulase in a mass ratio of 2:1.
Example 6
The seaweed extract composition provided in this example comprises, by mass%, 13% of the seaweed extract obtained in example 1 (13 kg), 10% of the mulberry leaf extract (10 kg), 7% of green tea powder (7 kg), 7% of coffee powder (7 kg), 7% of green coffee powder (2 kg), 3% of L-carnitine powder (3 kg), 3% of Opuntia ficus-indica powder (3 kg), 40% of sorbitol (40 kg), 8% of microcrystalline cellulose (8 kg), 0.7% of essence for food (0.7 kg) and 1.3% of magnesium stearate (1.3 kg).
The preparation method comprises the following steps:
the seaweed extract, mulberry leaf extract, green tea powder, coffee powder, green coffee powder, L-carnitine powder and Opuntia ficus-indica powder prepared in example 1 were sieved with 80 mesh sieve respectively;
weighing sieving raw materials and related auxiliary materials (sorbitol, microcrystalline cellulose, food essence and magnesium stearate serving mainly to improve taste, increase tablet adhesiveness, disintegration property, smoothness and the like) according to the formula amount, putting into a three-dimensional mixer for mixing, controlling the mixing rotating speed at 15r/min, and mixing for 30min to obtain mixed powder;
the mixed powder was put into a hopper of a tabletting machine for tabletting, and tablets of 0.8 g/tablet were obtained.
Example 7
The seaweed extract composition provided in this example comprises, by mass, 13% of the seaweed extract prepared in example 1 (13 kg), 10% of the mulberry leaf extract (10 kg), 7% of green tea powder (7 kg), 7% of coffee powder (7 kg), 7% of green coffee powder (2 kg), 3% of L-carnitine powder (3 kg), 3% of Opuntia ficus-indica powder (3 kg), 40% of sorbitol (40 kg), 8% of microcrystalline cellulose (8 kg), 0.7% of food essence (0.7 kg) and 1.3% of magnesium stearate (1.3 kg).
The preparation method comprises the following steps:
the seaweed extract, mulberry leaf extract, green tea powder, coffee powder, green coffee powder, L-carnitine powder and Opuntia ficus-indica powder prepared in example 1 were sieved with 80 mesh sieve respectively;
weighing the sieving raw materials and related auxiliary materials according to the formula amount, putting into a three-dimensional mixer for mixing, controlling the mixing rotating speed at 15r/min, and mixing for 30min to obtain powder.
Example 8
The seaweed extract composition provided in this example comprises, by mass, 2.6% of the seaweed extract prepared in example 1 (2.6 kg), 2% of the mulberry leaf extract (2 kg), 1.4% of green tea powder (1.4 kg), 1.4% of coffee powder (1.4 kg), 1.4% of green coffee powder (1.4 kg), 0.6% of L-carnitine powder (0.6 kg), 0.6% of Opuntia ficus-indica powder (0.6 kg) and adjuvants: apple fruit powder 5% (5 kg), xanthan gum 0.2% (0.2 kg), sucralose 0.1% (0.1 kg), citric acid 0.3% (0.3 kg), apple essence 0.1% (0.1 kg), water 84.3% (84.3 kg).
The preparation method comprises the following steps:
the seaweed extract, mulberry leaf extract, green tea powder, coffee powder, green coffee powder, L-carnitine powder and Opuntia ficus-indica powder prepared in example 1 were sieved with 80 mesh sieve respectively;
weighing the sieving raw materials and the related auxiliary materials according to the formula amount, adding purified water into a batching tank, adding the sieving raw materials and the related auxiliary materials into the batching tank, and stirring and mixing for 30min to obtain compound batching liquid;
filtering the compound liquid through a filter element type filter; obtaining a filtered feed liquid;
sterilizing the filtered feed liquid at high temperature by UHT; obtaining a sterilizing feed liquid;
and (3) filling the sterilized feed liquid into the oral liquid by an automatic filling machine.
Test case
The test example is weight reduction, emission promotion and fat burning test
Experimental sample
The seaweed extract oral liquid obtained in example 8 was filled into a 45 mL/bag package to prepare a test substance having a seaweed extract composition mass concentration of 0.1 g/mL.
Experimental crowd
(1) Volunteer enrollment criteria
The age is 25-50 years.
(2) Overweight, loving exercise, high body fat rate or constipation, and fat reduction;
(3) continuously taking for four weeks, each time of 2 times in the morning and evening, 1 bag each time;
(2) Treatment regimen
Blank group: the functional components are not added, and only auxiliary materials (5 percent of apple fruit powder (5 kg), 0.2 percent of xanthan gum (0.2 kg), 0.1 percent of sucralose (0.1 kg), 0.3 percent of citric acid (0.3 kg), 0.1 percent of apple essence (0.1 kg) and 84.3 percent of water (94.3 kg)) are added for 20 cases.
Test group: the seaweed extract oral liquid is 45 mL/bag, 1 bag is taken each time, 1 bag is taken in the morning and evening, and 20 cases are taken in total.
(3) Application method
Both groups were orally administered in 2 bags per day. 1 bag is taken each time, 1 bag is taken in the morning and evening, and the administration is continued for 4 weeks.
(4) The test results are shown in tables 2 and 3.
Table 2 average body weight (kg) comparison between 2 and 4 weeks after dosing prior to the two groups of volunteers trial
Table 3 comparison of average body fat rates (%) at 2 and 4 weeks after dosing prior to the two groups of volunteer trials
As can be seen from the test example results, compared with the blank group, the test group has obvious effects of weight reduction, excretion promotion and fat reduction, and can be seen that after 2 weeks and 4 weeks of use, the test group has obvious weight loss and body fat rate reduction after 2 weeks and 4 weeks of use. Therefore, the seaweed extract composition provided by the invention can play a role in obviously burning fat and promoting fat metabolism; the seaweed extract composition is effective in increasing fat metabolism, inhibiting lipogenesis, and reducing body fat rate
Although the foregoing embodiments have been described in some, but not all embodiments of the invention, other embodiments may be obtained according to the present embodiments without departing from the scope of the invention.
Claims (7)
1. The seaweed extract composition is characterized by comprising the following components in parts by mass: 10-13 parts of seaweed extract, 1-30 parts of mulberry leaf extract, 5-7 parts of green tea, 1-20 parts of coffee, 1-20 parts of green coffee, 1-10 parts of L-carnitine and 1-15 parts of Opuntia ficus-indica; the average relative molecular weight of the seaweed extract is 1000-30000 Da; the mass percentage of fucoxanthin in the seaweed extract is 1.5-3wt%;
the preparation method of the seaweed extract comprises the following steps: mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and carrying out solid-liquid separation to obtain seaweed enzymolysis sediment; the complex hydrolase comprises alginic acid lyase and cellulase; dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on the leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid; concentrating the seaweed extract purified solution, and drying to obtain the seaweed extract.
2. The seaweed extract composition of claim 1, wherein the mass of the complex hydrolase is 0.5 to 1.0% of the fresh weight of fresh seaweed.
3. The seaweed extract composition of claim 1 or 2, wherein the mass ratio of the alginic acid lyase to the cellulase is (1-3): 1-3; the enzyme activity units of the alginic acid lyase and the cellulase are independently 5-50 ten thousand U/g.
4. The seaweed extract composition of claim 1, wherein the temperature of the enzymolysis is 35-60 ℃; the heat preservation time of the enzymolysis is 1-6 h, and the pH of the enzymolysis is 6-8.
5. The seaweed extract composition of claim 1, further comprising preparing an aqueous seaweed slurry from said fresh seaweed prior to said mixing, and pulverizing said aqueous seaweed slurry to obtain a slurry of seaweed particles; the granularity of seaweed particles in the seaweed particle slurry is 10-30 mu m.
6. The seaweed extract composition of claim 5, wherein the pulverization is colloid mill pulverization, and the power of the colloid mill pulverization is 37-45 w; the grinding speed of the colloid mill is 2000-3000 r/min.
7. The seaweed extract composition of claim 1, further comprising an adjunct comprising one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food flavors, xanthan gum, citric acid, povidone K30, and gelatin.
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