CN109354628A - A kind of preparation method and applications of xylo-oligosaccharide - Google Patents
A kind of preparation method and applications of xylo-oligosaccharide Download PDFInfo
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- CN109354628A CN109354628A CN201811579732.5A CN201811579732A CN109354628A CN 109354628 A CN109354628 A CN 109354628A CN 201811579732 A CN201811579732 A CN 201811579732A CN 109354628 A CN109354628 A CN 109354628A
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- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
- C08B30/18—Dextrin, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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Abstract
The invention discloses a kind of preparation methods of xylo-oligosaccharide, the following steps are included: freezing makes cell wall rupture after corncob alkalinity is crushed, it is subsequently placed under acidic environment and is ultrasonically treated, it is spare successively to obtain xylan solution after AMBERJET 1200H and Amberlite XAD-16 processing；Xylan solution is digested using zytase, obtains xylo-oligosaccharide solution, xylo-oligosaccharide solution is crystallized.The xylan solution high income that the present invention obtains, to make the yield for improving xylo-oligosaccharide, and preparation method provided by the invention is simple to operation.
Description
Technical field
The invention belongs to chemical industry, food technology field, and in particular to a kind of preparation method and applications of xylo-oligosaccharide.
Background technique
Xylo-oligosaccharide is also known as wood oligose, is the functional polymerization being combined by 2-7 xylose molecule with β-Isosorbide-5-Nitrae glycosidic bond
Sugar, xylo-oligosaccharide are that one of strongest kind of Bifidobacterium function is proliferated in polymerization carbohydrate, and xylo-oligosaccharide has many unique
Function: 1, xylo-oligosaccharide is difficult to human digestive enzymes and is decomposed, and carries out digestion experiment with saliva, gastric juice, pancreatic juice and small intestine enzyme solution,
Experimental result shows that various digestive ferments cannot nearly all decompose xylo-oligosaccharide, and the energy value of xylo-oligosaccharide is almost nil, both
Blood sugar concentration is not influenced, the level of insulin in blood glucose will not be increased, and not will form fat deposition, therefore can be in low energy
It plays a role in food, meets diabetes patient, adiposis patient and hypoglycemosis human needs to greatest extent；2, xylo-oligosaccharide
Good to acid, thermal stability, hardly possible fermentation, compared with other oligosaccharide, the outstanding feature of xylo-oligosaccharide is that stability is very good, even if
(pH=2.5-7) is heated to 100 DEG C and does not also decompose substantially in acid condition.Xylo-oligosaccharide is to be proliferated bifid in polymerization carbohydrate
One of strongest kind of bacillus function, its effect of property are nearly 20 times of other polymerization carbohydrates, are not hydrolyzed in human gastrointestinal tract
The enzyme of xylo-oligosaccharide promotes Bifidobacterium proliferation simultaneously so it is preferably that Bifidobacterium is utilized that it, which can be directly entered big enteral,
Generate a variety of organic acids.Enteron aisle pH value is reduced, inhibits harmful bacteria growth, is proliferated probiotics largely in enteron aisle, reaches above-mentioned
Health-care efficacy.
Xylo-oligosaccharide is widely used, and can be used for feed additive field, role are as follows: 1, promotion animal alimentary canal
The breeding of interior beneficial bacterium inhibits the growth of harmful bacteria, improves microbial flora balance, 2, blocking pathogen and host cell receptor
Combination, 3, stimulate animal matrix immune system, improve immunity, 4, enhance animal disease resistance, reduce disease incidence, mention
Control efficiency of the height to Animal diseases, 5, improvement efficiency of feed utilization, raising breeding performonce fo animals, 6, pollution of the reduction to environment,
Improve the sanitary condition of animal productiong environment；In terms of can be also used for mother and baby's articles, xylo-oligosaccharide is in terms of infant's product
Using concentrating on glucose product, milk powder product etc..
Since the application range of xylo-oligosaccharide is wider, so preparation quality height, high-quality xylo-oligosaccharide become in weight
Weight, the preparation method of xylo-oligosaccharide are broadly divided into enzymatic hydrolysis, Acid hydrolysis, digest four kinds from hydrolysis, chemistry, enzymatic hydrolysis
Since clean and environmental protection becomes the main preparation methods of xylo-oligosaccharide, but certain drawbacks are equally existed, such as preparation condition control
It makes the improper enzyme that will lead to inactivate, or enzyme and reactant binding site are insufficient in reaction, reaction is not thorough, so as to cause
The xylo-oligosaccharide yield of synthesis is low, purity difference.
Such as a kind of preparation method of xylo-oligosaccharide is disclosed in Chinese patent application 201610217884X, belong to oligomeric
Technical field of xylose production, this method will obtain xylan by alkalinity extraction in the agricultural fibre waste material containing hemicellulose,
In the aqueous solution of xylan, addition solid acid catalyst, which is directly degraded, obtains xylo-oligosaccharide solution, after filtering by solid acid
Catalyst and the isolated xylo-oligosaccharide of xylo-oligosaccharide solution, this method low cost, green production, environmental-friendly, easy behaviour
The product quality make, obtained is high, and the yield of this method xylan is low, to keep the yield of xylo-oligosaccharide relatively low.
For another example, it is disclosed in Chinese patent application 201410330909.3 and a kind of prepares xylo-oligosaccharide with sugar grass fermentation residue
Method, belong to technical field of biochemical industry.Method provided by the invention be using sugar grass fermentation residue as raw material, it is pre- by alkaline process
It after handling and being separated by solid-liquid separation, then after obtained solid phase is washed to neutrality, is separated by solid-liquid separation after being saccharified with zytase, then by super
Filter method obtains xylo-oligosaccharide liquid glucose, then after activated carbon adsorption decoloration and ion-exchange resin purification, after concentration addition excipient
Spray drying.Method provided by the invention takes full advantage of remaining fermentation residue after sugar grass solid state fermentation production alcohol fuel,
The problem that impurity is more in acid-catalyzed hydrolysis technique is overcome, therefore subsequent xylose purifying cost, the oligomeric wood of gained is effectively reduced
Sugar products matter is good, and recovery rate is high, but the preparation method operating procedure of the disclosure of the invention is complicated, instrument it is more, cost compared with
It is high.
With the continuous improvement of people's living standards, fine food starts gradually to substitute other foods and becomes staple food, dining table
On meat and vegetables ratio also start gradually to occur the inclination of morbid state, this to need in more and more people, especially city often
The overtime work of property, the white collars for paying close attention to healthy diet of having no time, enter " inferior health " state.Cardiovascular disease, obesity, intestines problem
The problems such as start generally to perplex today's society, this makes healthy diet initially enter the visual field of more people.
Resistant dextrin, stachyose, L-arabinose, soyabean oligosaccharides, chitosan oligosaccharide, oligomeric dragon gallbladder sugar, Oligomeric manna sugar etc.
Prebiotics do not have relevant enzyme and environment to be degraded, absorbed in upper digestive tract, and into after enteron aisle, certain by large intestine specific have
Beneficial bacteria carries out decomposition digestion, promotes the growth of beneficial bacterium, inhibits the number of harmful bacteria.It absorbs water and is digested drop after entering large intestine
Solution is gas and acidic materials.It water suction and produces gas and can increase intestinal contents volume, reduce its storage and stay the time and avoid constipation
Occur, the acidic materials of generation can then adjust the pH value of enteron aisle, to inhibit the growth of harmful bacteria and reduce intestines problem
Occur.The ability that cholesterol, raising body in serum resist the disease can be reduced by taking prebiotics simultaneously, and people is helped to walk out
" inferior health " state.
But there are certain barriers in actual industrial application for prebiotics additive.Due to resistant dextrin, stachyose, L-
The prebiotics such as arabinose, soyabean oligosaccharides, chitosan oligosaccharide, oligomeric dragon gallbladder sugar, Oligomeric manna sugar are functional oligosaccharide, i.e.,
The oligomer of polyhydroxy aldehyde or polyhydroxyketone has very strong hygroscopicity, while its reducing end group also assigns these oligosaccharide one
Fixed reactivity is easy if directly using or adding prebiotics or prebiotics powder in processing, use, storage, addition
Occur Deng during moisture absorption, occur it is hardened, or even during storage with amino acid, protein in other adding ingredients etc.
Complex chemical reaction occurs for active part, is unfavorable for the production and use of product, needs to protect it.But it is existing to pass through
Prepare that the methods of micro-capsule then has the control of production is at high cost, is unfavorable for production process quality, poor universality is difficult to popularize
The disadvantages of to a variety of prebiotics Additive Productions.Known protection materials are mostly at high price simultaneously, and in wet environments such as kitchens
In be still prone to it is hardened.
In order to solve probiotics technical problems such as the generally existing easy moisture absorption, easy to harden during production and storage etc.,
A kind of prebiotics additive and its preparation method and application is disclosed in Chinese patent application 201711038208.2.Comprising prebiotic
First sugar and modified maltodextrin；The modification maltodextrin is obtained by maltodextrin through alcoholic solution recrystallization, heat treatment.This hair
The bright preparation method for also disclosing the additive and the additive is used to prepare the edible salt comprising prebiotics, solid
Application in the fermented teas such as beverage and dark green tea, black tea.The present invention originally proposes that one kind is changed by physics by numerous studies
Property maltodextrin solves the thinking of the technical problem.In the present invention, the physical modification is carried out to maltodextrin, it can
It realizes its interior molecules rearrangement, the degree of unfolding for changing strand, remove the purpose of unstable low molecular sugar, malt is pasted
Essence intermolecular gap, hydrophilic group arrangement etc. have improvement effect.It, can be effective using the modification maltodextrin of the physical modification
Reduce hygroscopicity, the hardened incidence of prebiotics additive.But prebiotics used in this application are single prebiotics, are being produced
Significant limitation is still remained in product.
Few for above-mentioned xylo-oligosaccharide yield, the shortcomings and deficiencies of preparation process complexity, it is simple to develop a kind of step, operation
Simplicity, the preparation method of xylo-oligosaccharide high income, convenient for wide in the especially high-end food of food, health care product and field of health care products
General application.
Summary of the invention
In view of the deficiency of the prior art, a kind of preparation method of xylo-oligosaccharide is provided, this method has step letter
It is single, it is easy to operate, the advantages that xylo-oligosaccharide high income.
A kind of preparation method of xylo-oligosaccharide, comprising the following steps:
Successively are as follows: raw material is crushed, the absorption of ultrasonic treatment, cation seperation column, anion column absorption, digested, crystallization；
The raw material crushes are as follows: crushes corncob, is put into obturator and is freezed, then room temperature condition
Under dissolve it naturally, the corncob after obtaining freezing processing is spare；
The ultrasonic treatment are as follows: the corncob after freezing processing adjusts pH value with acid after adding water and surpasses in a heated condition
Sound, filtering, to obtain supernatant spare for centrifugation；
The cation seperation column absorption are as follows: supernatant is cleaned by cation exchange resin column, eluent is obtained, with work
Property charcoal adsorbed, filtering and collecting filter liquid, vacuum concentration, obtain concentrate；
The anion column absorption are as follows: concentrate is cleaned by anion-exchange resin column, eluent is obtained, adjusts
It is spare that eluent pH value obtains enzymatic hydrolysis xylan solution；
The enzymatic hydrolysis are as follows: enzymatic hydrolysis xylan solution is digested using zytase, obtains xylo-oligosaccharide solution；
The crystallization are as follows: xylo-oligosaccharide solution is concentrated in vacuo to saturated solution, is then crystallized to get oligomeric wood
Sugar.
The temperature freezed in the raw material crushing is -15--20 DEG C, and cooling time is 20-30 minutes.
The pH value adjusted in the ultrasonic treatment is 3.5-5.5, and Ultrasonic Heating temperature is 80-100 DEG C, and ultrasonic time is
1-2.5 hour；
Adsorption time in the cation seperation column absorption is 20-40 minutes；
PH value in the anion column absorption is 3.5-5.5；
The temperature of vacuum concentration in the crystallization is 50-80 DEG C.
The cation seperation column absorption is carried out using AMBERJET 1200H.
The anion column absorption is carried out using Amberlite XAD-16.
In order to further solve single prebiotics existing limitation in the product, the present invention also provides a kind of compound benefits
Raw member, the purpose of the complex prebiotics are to control weight and improve colony balance.
It is a kind of to improve the complex prebiotics composition of colony balance for controlling weight, it according to parts by weight include with the following group
Point:
Preferably, above-mentioned to improve the complex prebiotics composition of colony balance for controlling weight, it wraps according to parts by weight
Include following components:
It is further preferred that above-mentioned improve the complex prebiotics composition of colony balance for controlling weight, by weight
Number meter includes following components:
In a preferred embodiment, above-mentioned to improve the complex prebiotics composition of colony balance for controlling weight, it presses
Parts by weight meter includes following components:
It is in a further advantageous embodiment, above-mentioned to improve the complex prebiotics composition of colony balance for controlling weight,
According to parts by weight include following components:
Above-mentioned complex prebiotics composition, the ratio between the resistant dextrin, poly- galactolipin and parts by weight of oligofructose
For 5-10:1:1；Preferably, the ratio between parts by weight of the resistant dextrin, poly- galactolipin and oligofructose are 6-9:1:1；Into
Preferably, the ratio between parts by weight of the resistant dextrin, poly- galactolipin and oligofructose are 7-8:1:1 to one step.
Above-mentioned complex prebiotics composition, it is described in the ratio between parts by weight of short chain inulin and long-chain inulin be 1:1.
The weight fraction ratio of above-mentioned complex prebiotics composition, the oligofructose and xylo-oligosaccharide is 1-7:1；It is preferred that
The weight fraction ratio of ground, the oligofructose and xylo-oligosaccharide is 2-6:1；It is further preferred that the oligofructose and
The weight fraction ratio of xylo-oligosaccharide is 3-5:1.
Above-mentioned complex prebiotics composition can be applied to food, food additives, health food, human food, veterinary drug, move
In terms of object feed.
Above-mentioned complex prebiotics composition is applied to prebiotics solid beverage in a preferred embodiment.
The present invention also provides the preparation method of above-mentioned complex prebiotics solid beverage, specific steps are as follows:
By formulation weight part weigh resistant dextrin, poly- galactolipin, oligofructose, in short chain inulin, long-chain inulin, stachyose
It is mixed at room temperature with xylo-oligosaccharide by certain proportion, obtains mixed-powder, a certain amount of water is added into mixed-powder, surpassed
Sound 10-30min is dissolved under the conditions of 20-40 DEG C, is filtered, it is sterilized after concentration, freeze-drying, 20-35 DEG C of low-temperature grinding pass through
Packaging is prepared after 100-200 mesh.
Amount of water described in above-mentioned steps is just to be completely dissolved mixed-powder.
Cooling time described in above-mentioned steps is 18-36h.
Beneficial effects of the present invention:
(1) complex prebiotics solid beverage disclosed by the invention makes each by adjusting the type and proportion of various prebiotics
It can interact between kind prebiotics, can preferably promote the increase of organic acid and short chain fatty acids in enteron aisle, adjust intestines
Road flora composition, inhibits harmful bacteria, increases beneficial to bacterial content.
(2) the adjustable fat metabolism of complex prebiotics solid beverage disclosed in the present invention increases energy consumption, lowers
Appetite reduces food ration, reduces cholesterol, and triglycerides increases mineral absorption, mitigates inflammation, loses weight.
(3) prebiotics solid beverage disclosed by the invention not only plays the role of improving colony balance, also has stimulation immune
Cell, strengthen immunity effect.
Specific embodiment
A kind of basic embodiment 1: preparation method of xylo-oligosaccharide
The following steps are included: successively are as follows: raw material crush, ultrasonic treatment, cation seperation column absorption, anion column absorption, enzymatic hydrolysis,
Crystallization；
The raw material crushes are as follows: crushes corncob, is put into obturator and is freezed, then room temperature condition
Under dissolve it naturally, the corncob after obtaining freezing processing is spare；
The ultrasonic treatment are as follows: the corncob after freezing processing adjusts pH value with acid after adding water and surpasses in a heated condition
Sound, filtering, to obtain supernatant spare for centrifugation；
The cation seperation column absorption are as follows: supernatant is cleaned by cation exchange resin column, eluent is obtained, with work
Property charcoal adsorbed, filtering and collecting filter liquid, vacuum concentration, obtain concentrate；
The anion column absorption are as follows: concentrate is cleaned by anion-exchange resin column, eluent is obtained, adjusts
It is spare that eluent pH value obtains enzymatic hydrolysis xylan solution；
The enzymatic hydrolysis are as follows: enzymatic hydrolysis xylan solution is digested using zytase, obtains xylo-oligosaccharide solution；
The crystallization are as follows: xylo-oligosaccharide solution is concentrated in vacuo to saturated solution, is then crystallized to get oligomeric wood
Sugar.
The temperature freezed in the raw material crushing is -15--20 DEG C, and cooling time is 20-30 minutes.
The pH value adjusted in the ultrasonic treatment is 3.5-5.5, and Ultrasonic Heating temperature is 80-100 DEG C, and ultrasonic time is
1-2.5 hour；
Adsorption time in the cation seperation column absorption is 20-40 minutes；
PH value in the anion column absorption is 3.5-5.5；
The temperature of vacuum concentration in the crystallization is 50-80 DEG C.
The cation seperation column absorption is carried out using AMBERJET 1200H.
The anion column absorption is carried out using Amberlite XAD-16.
Basic embodiment 2-3 is on the basis of basic embodiment 1, by the specific implementation of below table adjusting parameter composition
Example:
Embodiment 1 is a kind of to improve the complex prebiotics composition of colony balance for controlling weight
Formula: including following components according to parts by weight:
Preparation method: specific steps are as follows:
Weigh 40 parts of resistant dextrin, 6 parts of poly- galactolipin, 6 parts of oligofructose, in 4 parts of short chain inulin, 4 parts of long-chain inulin, water
8 parts and 6 parts of xylo-oligosaccharide of threose mix at room temperature, obtain mixed-powder, water is added into mixed-powder, keep mixed-powder complete
Fully dissolved, ultrasonic 10min dissolve under the conditions of 40 DEG C, filter, it is sterilized after concentration, be freeze-dried 18h, 20 DEG C of low-temperature grindings are led to
Packaging is prepared after sieving with 100 mesh sieve.
Embodiment 2 is a kind of to improve the complex prebiotics composition of colony balance for controlling weight
Formula: including following components according to parts by weight:
Preparation method: specific steps are as follows:
Weigh 60 parts of resistant dextrin, 12 parts of poly- galactolipin, 12 parts of oligofructose, in 6 parts of short chain inulin, 6 parts of long-chain inulin,
2 parts and 4 parts of xylo-oligosaccharide of stachyose mix at room temperature, obtain mixed-powder, water is added into mixed-powder, make mixed-powder
It is completely dissolved, ultrasonic 20min, is dissolved under the conditions of 35 DEG C, filtered, is concentrated after sterilized, is freeze-dried for 24 hours, 25 DEG C of low-temperature grindings,
It is prepared by being packed after 120 meshes.
Embodiment 3 is a kind of to improve the complex prebiotics composition of colony balance for controlling weight
Formula: including following components according to parts by weight:
Preparation method: specific steps are as follows:
Weigh 50 parts of resistant dextrin, 10 parts of poly- galactolipin, 10 parts of oligofructose, in 5 parts of short chain inulin, 5 parts of long-chain inulin,
6 parts and 2 parts of xylo-oligosaccharide of stachyose mix at room temperature, obtain mixed-powder, water is added into mixed-powder, make mixed-powder
It is completely dissolved, ultrasonic 25min, is dissolved under the conditions of 30 DEG C, filtered, is concentrated after sterilized, freeze-drying 30h, 30 DEG C of low-temperature grindings,
It is prepared by being packed after 150 meshes.
Embodiment 4 is a kind of to improve the complex prebiotics composition of colony balance for controlling weight
Formula: including following components according to parts by weight:
Preparation method: specific steps are as follows:
Weigh 45 parts of resistant dextrin, 8 parts of poly- galactolipin, 8 parts of oligofructose, in 6 parts of short chain inulin, 6 parts of long-chain inulin, water
4 parts and 5 parts of xylo-oligosaccharide of threose mix at room temperature, obtain mixed-powder, water is added into mixed-powder, keep mixed-powder complete
Fully dissolved, ultrasonic 30min dissolve under the conditions of 35 DEG C, filter, it is sterilized after concentration, for 24 hours, 20 DEG C of low-temperature grindings are led to for freeze-drying
It crosses after 200 meshes to pack and be prepared.
Embodiment 5 is a kind of to improve the complex prebiotics composition of colony balance for controlling weight
Formula: including following components according to parts by weight:
Preparation method: specific steps are as follows:
Weigh 55 parts of resistant dextrin, 10 parts of poly- galactolipin, 10 parts of oligofructose, in 5 parts of short chain inulin, 5 parts of long-chain inulin,
3 parts and 4 parts of xylo-oligosaccharide of stachyose mix at room temperature, obtain mixed-powder, water is added into mixed-powder, make mixed-powder
It is completely dissolved, ultrasonic 20min, is dissolved under the conditions of 40 DEG C, filtered, is concentrated after sterilized, freeze-drying 36h, 35 DEG C of low-temperature grindings,
It is prepared by being packed after 150 meshes.
A kind of complex prebiotics composition of comparative example 1
Formula: it is with the difference of embodiment 1: the weight fraction ratio of institute's addition polymerization galactolipin, oligofructose and resistant dextrin
For 1:1:3.7.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 1.
A kind of complex prebiotics composition of comparative example 2
Formula: it is with the difference of embodiment 1: the weight fraction ratio of institute's addition polymerization galactolipin, oligofructose and resistant dextrin
For 1:1:12.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 1.
A kind of complex prebiotics composition of comparative example 3
Formula: be with the difference of embodiment 3: the weight fraction ratio of short chain inulin and long-chain inulin is 1:4 in added.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 3.
A kind of complex prebiotics composition of comparative example 4
Formula: be with the difference of embodiment 3: the weight fraction ratio of short chain inulin and long-chain inulin is 4:1 in added.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 3.
A kind of complex prebiotics composition of comparative example 5
Formula: be with the difference of embodiment 5: the weight fraction ratio of added middle oligofructose and xylo-oligosaccharide is 0.5:1.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 5.
A kind of complex prebiotics composition of comparative example 6
Formula: be with the difference of embodiment 5: the weight fraction ratio of added middle oligofructose and xylo-oligosaccharide is 8:1.
Preparation method: in addition to formulation weight number is different, other operations are same as Example 5.
Experimental section
1, dissolubility is tested:
The complex prebiotics solid beverage of embodiment 1-5 is added in about 40 DEG C of water and is gently mixed, about in 1-2min
Inside it is completely dissolved.
2, security test:
10 volunteers are found, respectively the complex prebiotics solid beverage 10-20g of daily early oral meal embodiment 1-5,
The side effects such as diarrhea are not found.
3, beneficial effect is tested
According to Dalian Medical Univ's Zhou Jingxin master thesis, " several prebiotic preparations make intestinal flora to experimental method
With the research of effect " method of experiment in vitro and dosage are tested in the paper of code Y1044452, and ferment 12 hours hindguts
Road Microflora measurement, as a result such as the control group phase of following table each embodiment group intestinal flora and the glucose for being incorporated as same dose
Compare, enterobacteria, enterococcus are inhibited (P > 0.05), and Bifidobacterium, Bacillus acidi lactici increase significant (P < 0.01), and data are said
To harmful bacteria enterobacteria, enterococcus is inhibited, increase beneficial bacterium Bifidobacterium, Bacillus acidi lactici have aobvious the formula of bright embodiment 1-5
Write effect.The influence to human body intestinal canal B/E value in vivo studies 14 days is carried out according to the method for above-mentioned paper, matching described in discovery can
To improve human body intestinal canal B/E value, be conducive to stablize intestinal microbial balance.
Data processing method
Flora investigates bacterium viable count logN,It indicates, data processing method is using at SPSS11.0 statistical software
Reason.
(1) results from vivo experiments
Table 1 ferment 12 hours rear intestinal Microfloras (lgCFU/g, it is wet just, average ± standard deviation, n=5) see the table below 1
Table 1
After thering is the detection data of upper table 1 to can be seen that fermentation 12 hours, intestinal microflora measurement, each experimental group enteron aisle
Bifidobacterium, lactobacillus, enterobacteria, enterococcus increase flora compared with the control group, but lactobacillus and Bifidobacterium increase
Significantly (P < 0.01), that is to say, that complex prebiotics solid beverage disclosed by the invention, by adjusting the type of various prebiotics
And proportion, make to interact between various prebiotics, can preferably promote organic acid and short chain fatty acids in enteron aisle
Increase, adjusts intestinal flora composition, inhibit the content of harmful bacteria (enterobacteria, enterococcus), increase beneficial bacterium (Bifidobacterium, cream
Bacillus) content.
(2) experiment in vivo result
Prebiotics solid beverage prepared by the present invention is continuously taken after 14 days to the measurement (lgCFU/ of human body intestinal canal B/E value
G, it is wet just, average ± standard deviation, n=8) see the table below 2
Table 2
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Before taking | 1.10±0.24 | 1.08±0.27 | 1.09±0.25 | 1.12±0.20 | 1.09±0.27 |
| After taking | 1.35±0.25 | 1.34±0.24 | 1.32±0.22 | 1.30±0.28 | 1.32±0.23 |
P < 0.05 compared with before take.
Each flora of five groups of subject's enteron aisles before taking prebiotic preparation counts no significant difference (P > 0.05).It is eating
No significant difference (P > 0.05) is counted with each flora of enteron aisle between each group after prebiotics solid beverage prepared by the present invention 14 days.
Complex prebiotics composition prepared by the present invention can also control weight well, even the crowd of easily fatting habitus
It takes for long periods of time under can also controlling weight well in certain weight range, is not in obviously increasing for weight.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of xylo-oligosaccharide, it is characterised in that: the following steps are included:
Successively are as follows: raw material is crushed, the absorption of ultrasonic treatment, cation seperation column, anion column absorption, digested, crystallization；
The raw material crushes are as follows: crushes corncob, is put into obturator and is freezed, then makes under room temperature
It is dissolved naturally, and the corncob after obtaining freezing processing is spare；
The ultrasonic treatment are as follows: the corncob after freezing processing uses acid adjustment pH value and in a heated condition ultrasound after adding water,
Filtering, to obtain supernatant spare for centrifugation；
The cation seperation column absorption are as follows: supernatant is cleaned by cation exchange resin column, eluent is obtained, uses active carbon
It is adsorbed, filtering and collecting filter liquid, is concentrated in vacuo, obtains concentrate；
The anion column absorption are as follows: concentrate is cleaned by anion-exchange resin column, obtains eluent, adjustment elution
It is spare that liquid pH value obtains enzymatic hydrolysis xylan solution；
The enzymatic hydrolysis are as follows: enzymatic hydrolysis xylan solution is digested using zytase, obtains xylo-oligosaccharide solution；
The crystallization are as follows: xylo-oligosaccharide solution is concentrated in vacuo to saturated solution, is then crystallized to get xylo-oligosaccharide.
2. preparation method according to claim 1, it is characterised in that: the temperature freezed in the raw material crushing
It is -15--20 DEG C, cooling time is 20-30 minutes.
3. preparation method according to claim 1, it is characterised in that: the pH value adjusted in the ultrasonic treatment is 3.5-
5.5。
4. preparation method according to claim 1, it is characterised in that: the Ultrasonic Heating temperature is 80-100 DEG C, is surpassed
The sound time is 1-2.5 hours.
5. preparation method according to claim 1, it is characterised in that: the adsorption time in the described cation seperation column absorption is
20-40 minutes.
6. preparation method according to claim 1, it is characterised in that: the pH value in the anion column absorption is 3.5-
5.5。
7. preparation method according to claim 1, it is characterised in that: the temperature of the vacuum concentration in the crystallization is
50-80℃。
8. preparation method according to claim 1, it is characterised in that: the cation seperation column absorption uses AMBERJET
1200H is carried out.
9. preparation method according to claim 1, it is characterised in that: the anion column absorption uses Amberlite
XAD-16 is carried out.
10. the xylo-oligosaccharide of -9 described in any item preparation method preparations changes in preparation for controlling weight according to claim 1
Application in the complex prebiotics composition of kind colony balance.
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| CN2018107163794 | 2018-07-03 | ||
| CN201810716379 | 2018-07-03 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110237092A (en) * | 2019-06-11 | 2019-09-17 | 厦门蓝特生物科技有限公司 | A kind of composition for treating constipation |
| CN111493306A (en) * | 2020-06-05 | 2020-08-07 | 山东肥城精制盐厂有限公司 | Xylo-oligosaccharide edible salt capable of effectively improving moisture resistance |
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| CN102206691A (en) * | 2011-03-26 | 2011-10-05 | 北海田野食品有限公司 | Method for producing xylose oligomer from bagasse |
| CN103053903A (en) * | 2012-12-29 | 2013-04-24 | 北京中科邦尼国际科技有限责任公司 | Compound functional sugar with function of reducing food glycemic indexes |
| CN104642870A (en) * | 2015-02-05 | 2015-05-27 | 骆奇 | Prebiotics composition |
| CN105085697A (en) * | 2015-08-05 | 2015-11-25 | 都宝君 | Chlorella polysaccharide extraction method |
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|---|---|---|---|---|
| CN102206691A (en) * | 2011-03-26 | 2011-10-05 | 北海田野食品有限公司 | Method for producing xylose oligomer from bagasse |
| CN103053903A (en) * | 2012-12-29 | 2013-04-24 | 北京中科邦尼国际科技有限责任公司 | Compound functional sugar with function of reducing food glycemic indexes |
| CN104642870A (en) * | 2015-02-05 | 2015-05-27 | 骆奇 | Prebiotics composition |
| CN105085697A (en) * | 2015-08-05 | 2015-11-25 | 都宝君 | Chlorella polysaccharide extraction method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110237092A (en) * | 2019-06-11 | 2019-09-17 | 厦门蓝特生物科技有限公司 | A kind of composition for treating constipation |
| CN111493306A (en) * | 2020-06-05 | 2020-08-07 | 山东肥城精制盐厂有限公司 | Xylo-oligosaccharide edible salt capable of effectively improving moisture resistance |
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