CN108013237A - High-effective service and its application in lamb supplement material is prepared - Google Patents
High-effective service and its application in lamb supplement material is prepared Download PDFInfo
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Abstract
The invention discloses high-effective service and its prepare lamb supplement material in application, belong to technique for producing feed field, the high-effective service is prepared by culture medium, is inoculated with, compound bacteria culture and etc. be prepared；The high-effective service includes following raw material：Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, potassium phosphate, Trametes versicolor, microbacterium, saccharomycete；The present invention also provides application of the high-effective service in lamb supplement material is prepared, the percentage of the lamb supplement material either palatability, gaining in weight of lamb rate, lamb sick rate and the lamb neutrophil that are prepared using the high-effective service is better than feed made from the prior art；And supplemented and expected by compound bacteria-fermented lamb so that the active ingredient and amino acid of Chinese herbal medicine extract, trace element spread or penetrate into supplement material well, improve the nutritive value and disease resistance of supplement material.
Description
Technical field
The present invention relates to technique for producing feed field, more particularly to high-effective service and its in lamb supplement material is prepared
Application.
Background technology
Lamb stomach volume is small, and digestion fiber ability is weaker.1-20 days old lambs are that can only rely on breast milk without the stage of ruminating
Meet nutritional need, and the forage that cannot search for food；Lamb after 20 ages in days just starts to occur ruminating phenomenon, and can search for food a small amount of plant
Physical property feed, carries out Fiber Digestion decomposition, therefore suitably supplements some to lamb in nursing period and hold digestible plant feed,
Nourishing the stomach can be promoted to develop, make lamb do sth. in advance to adapt to the feeding environment of plant feed, create conditions for early weaning.But due to
Lamb resistivity is not strong and antibacterial ability is weaker, and the illnesss such as enteritis, occurs in easy bacterial infection when eating feed；Therefore need
The formula and preparation process for wanting lamb supplement material are extremely particular about, and the general of lamb infection germ is reduced in should expecting as far as possible from supplement
Rate, improves lamb disease resistance.
Chinese herbal medicine comes from natural plants, and toxic side effect is small, the katabolism easily in animal body, good drug efficacy, livestock products medicine
Thing residual quantity is low and some Chinese herbal medicines have the function that to promote growth of animal etc., in order to reduce the sick probability of livestock and poultry, improves body
Immunity, generally improves immunity of organisms using antibiotic is added in feed, and improvement is grown, but antibiotic etc
Long-time service can make microorganism produce drug resistance, cause livestock and poultry endogenous infection and two degree of infection, decline immunity of livestock, antibiosis
The problems such as element residual, seriously affect animal health and health.At present, more and more antibiotic are prohibited from using；Therefore,
Chinese herbal medicine is quoted to develop the non-stimulated new high disease-resistant feed of new type of safe for alleviating medicament residue and pathogenic bacteria resistance to drugs
Deng feed and food-safety problem, ensure that human health has positive meaning.
But current application of the Chinese herbal medicine in feed is added substantial amounts of in traditional Chinese herbal feed there is also some problems
Chinese herbal medicine, so as to have impact on the palatability of feed, Chinese patent 201510143866.2 discloses one kind in applying and carries new height
Disease resistance Chinese herbal medicine sheep medicine feed and preparation method thereof, which is made of the raw material of following parts by weight：：Soya-bean cake 40-
120 parts, 30-60 parts of wheat bran, 30-60 parts of rice bran, 10-30 parts of pears slag, 10-20 parts of treaster, 2-6 parts of salt；Chinese herbal medicine adds
1-10 parts of agent, is prepared by the preparation and fermenting raw materials of Chinese herb feed additive.It is general that merga pass is mixed using 18 kinds of Chinese herbal medicines
The mode that logical oiling decocts obtains Chinese herb feed additive, although preparation process is simple, the addition meetings of a large amount of Chinese herbal medicines
Bitter taste is produced, influences the palatability of feed, and Chinese herbal medicine effective ingredients utilization rate is low, therefore, it is necessary to the preparation of development of new
Technique, lowers the bitter taste for even removing Chinese herbal medicine, improves the palatability of feed, while make it that the active principle of Chinese herbal medicine is very big
Be dissolved into feed, improve the utilization rate of Chinese herbal medicine effective ingredients.
The disclosure of background above technology contents is only used for inventive concept and the technical solution that auxiliary understands the present invention, it is not
The prior art of present patent application is necessarily belonged to, shows the applying date of the above in present patent application in no tangible proof
In the case of disclosed, above-mentioned background technology should not be taken to the novelty and creativeness of evaluation the application.
The content of the invention
Present invention aims at the application for proposing a kind of high-effective service and its in lamb supplement material is prepared, with solution
State the technical problems such as middle feed palatability is poor, disease resistance is low；The lamb supplement prepared at the same time using high-effective service of the present invention
Material nutrient enriches, and can effectively realize lamb getting fat.
To solve the above problems, the present invention provides following technical scheme：
High-effective service, prepared, be inoculated with by culture medium, compound bacteria culture and etc. be prepared；
The high-effective service includes following raw material：Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, potassium phosphate, discoloration
Bolt bacterium, microbacterium, saccharomycete.
Further, the Trametes versicolor, microbacterium, the ratio of saccharomycete are：1:1:0.5.
Further, the Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, the amount of potassium phosphate are respectively：Sucus Caulis et Folium Brassicae capitatae
0.5g/l-1g/l, peptone 2.5g/l-5g/l, glucose 0.7g/l-1.2g/l, sodium chloride 0.4g/l-0.9g/l, potassium phosphate
0.8g/l-1.3g/l。
Further, the Sucus Caulis et Folium Brassicae capitatae 0.8g/l, peptone 3.8g/l, glucose 1.0g/l, sodium chloride 0.6g/l, phosphorus
Sour potassium 1.0g/l.
Further, the step specifically includes following：
T1：Prepare culture medium：Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, potassium phosphate are uniformly mixed, sterilizes, obtains
Obtain culture medium；
T2 is inoculated with：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, per 100g
In culture medium, inoculum concentration 0.5-1.0ml；
T3：Compound bacteria culture：The culture medium standing of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment
6-9d is cultivated, compound bacteria is made.
Further, shaken up manually once every 2d in the step T3 compound bacteria incubations.
The present invention also provides application of the above-mentioned high-effective service in lamb supplement material is prepared；The system of the lamb supplement material
Preparation Method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, mixed-powder and fishbone dust, the beans of gained
The dregs of rice, calcium carbonate are uniformly mixed, while are sprayed and added rice washing water, and mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino under the conditions of 28-30 DEG C, 600-800r/min
Acid, trace element, compound bacteria are uniformly mixed, then the material diaphragm seal that will be uniformly mixed, in 25-28 DEG C of temperature, humidity
Ferment 5-7d under the conditions of 60%-70%；
S3：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, and obtaining mesh number after sieving is
The lamb supplement material of 25-45 mesh.
Further, the addition of the step S2 compound bacterias is the 5-8% of mixture D mass fractions.
Further, the step S2 Chinese herbal medicine extracts are in units of parts by weight, including following raw material：5-7 parts of wrinkled giant hyssop,
6-8 parts of cordate houttuynia, 2-4 parts of coptis root, 8-10 parts of garlic, 2-4 parts of purple perilla, 8-10 parts of STEVIA REBAUDIANA, 6-8 parts of rhizoma zingiberis, 2-4 parts of Radix Codonopsis,
8-10 parts of radix glycyrrhizae, 7-9 parts of jujube.
Further, the Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 2-3cm, decocts 1 time at a temperature of 70-90 DEG C, and filtering, filter residue is again with the water of 2-3 times of parts by weight in 50-80 DEG C of temperature
Degree is lower to be decocted 1-2 times, filtering, merging filtrate；
D2：The filtrate that step D1 merges is concentrated under 10-15 DEG C, the cryogenic high pressure environment of pressure 1.02-1.5MPa
Relative density is the mixed liquor A of 1.20-1.30；
D3：Additional proportion is 0.5-0.7 in the mixed liquor A of step D2:The water of 1.0-1.2 and the mixed liquid B of ethanol, institute
The ratio for stating mixed liquor A and mixed liquid B is 1-2:0.8；40-55 DEG C of temperature, power 35-45W, frequency 20-25KHz ultrasound
20-30min is stirred under the conditions of ripple；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, is taken
Supernatant；
D5：In speed 1000-1500ml/h, 65-70 DEG C of feeding temperature, 30-40 DEG C of drop temperature, atomizing pressure 1.5-
Under the conditions of 2.15MPa, the supernatant of step D4 is spray-dried, obtains Chinese herbal medicine extract.
The beneficial effect that the present invention is compared with the prior art includes：
1st, the Trametes versicolor in high-effective service of the invention and microbacterium, which have, destroys cell membrane, degraded crude fibre and fruit
Glue etc. acts on, and can destroy sugarcane caudal lobe and straw while fermentation with the use of that can produce synergistic effect with saccharomycete
Cell membrane, degraded crude fibre in stalk so that the supplement material prepared is easier to be digested and assimilated by lamb；At the same time by compound
Bacterium is fermented so that and the active ingredient and amino acid of Chinese herbal medicine extract, trace element spread or penetrate into supplement material well,
Improve the nutritive value and disease resistance of supplement material.
2nd, wrinkled giant hyssop, cordate houttuynia, coptis root, garlic, purple perilla, STEVIA REBAUDIANA, rhizoma zingiberis, party in the herbal raw material that the present invention adds
Ginseng, radix glycyrrhizae, jujube play synergistic effect, and wrinkled giant hyssop is used cooperatively with coptis root, garlic, have change it is turbid and in, purging intense heat and detonicating, sterilization be good for
The effect of stomach, Radix Codonopsis, jujube are with the use of having a tonifying middle-Jiao and Qi, the effect of strengthen immunity, and cordate houttuynia is with antibacterial, disease-resistant
Poison, improve immunity of organisms and other effects, and rhizoma zingiberis can drive exterior cold, pre- avian influenza-prevention with purple perilla use；And STEVIA REBAUDIANA primarily serves
Seasoning acts on, and avoids the Chinese herbal medicine that boils excessively bitter, radix glycyrrhizae primarily serves the effect of coordinating the drug actions of a prescription so that ten taste Chinese herbal medicines are more
Add rational compatibility, it is respective the effect of reach maximization.
3rd, the feed prepared compared with the prior art, the Chinese herbal medicine extract that lamb supplement of the present invention is added in expecting can have
Effect improves the percentage of neutrophil in blood, you can to effectively improve the non-specific immunity of lamb, reduces life
Sick rate.
4th, lamb of the invention supplement material either palatability, gaining in weight of lamb rate, lamb sick rate and lamb neutrophilia is white
The percentage of cell is better than the prior art and feed is made.In addition, the later lamb of 30 ages in days should suitably feed supplement supplied materials,
To improve adaptability of the lamb to plant feed, while improve its immunity.
Embodiment
For ease of more fully understanding the present invention, the high-effective service of the present invention is used for the preparation process of lamb supplement material
In, and be illustrated by following embodiments, these embodiments belong to protection scope of the present invention, but do not limit the guarantor of the present invention
Protect scope.
Embodiment 1：
Lamb supplement material is in units of parts by weight, including following raw material：70 parts of sugarcane caudal lobe, 60 parts of peanut straw, fish-bone
70 parts of powder, 40 parts of dregs of beans, 10 parts of compound bacteria, 10 parts of rice washing water, 5 parts of calcium carbonate, 2 parts of amino acid/11, micro- 2 parts, Chinese herbal medicine
8 parts of extract；
The composite bacteria agent obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 0.5g/l, peptone 2.5g/l, glucose 0.7g/l, sodium chloride 0.4g/l, phosphorus
Sour potassium 0.8g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2 is inoculated with：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 0.5ml；
T3：Prepare compound bacteria：The culture medium standing training of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28 DEG C of environment
6d is supported, is during which shaken up manually once every 2d；Compound bacteria is made.
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：5 parts of wrinkled giant hyssop, 6 parts of cordate houttuynia, coptis root 2
Part, 8 parts of garlic, 2 parts of purple perilla, 8 parts of STEVIA REBAUDIANA, 6 parts of rhizoma zingiberis, 2 parts of Radix Codonopsis, 8 parts of radix glycyrrhizae, 7 parts of jujube；
The Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 2cm, decocts 1 time at a temperature of 70 DEG C, and filtering, filter residue decocts 1 with the water of 2 times of parts by weight at a temperature of 50 DEG C again
It is secondary, filtering, merging filtrate,
D2：By the filtrate that step D1 merges 10 DEG C of temperature, pressure 1.02MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.20 mixed liquor A；
D3：In the mixed liquor A of step D2 additional proportion be 0.51.0 water and ethanol mixed liquid B, the mixed liquor A
Ratio with mixed liquid B is 1:0.8；40 DEG C of temperature, power 35W, frequency 20KHz Ultrasonic Conditions under stir 20min；
To mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30 DEG C, rotating speed is 1200r/min, takes supernatant；
D5：Under the conditions of speed 1000ml/h, 65 DEG C of feeding temperature, 30 DEG C of drop temperature, atomizing pressure 1.5MPa, it will walk
The supernatant of rapid D4 is spray-dried, and obtains Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned lamb supplement material；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 25-
45 mesh；The mixed-powder of gained is uniformly mixed with fishbone dust, dregs of beans, calcium carbonate, while sprays and adds rice washing water, mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino acid, micro under the conditions of 28 DEG C, 600r/min
Element, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal in 25 DEG C of temperature, humidity 60%, without air stream
Ferment 5d under conditions of logical；
S3：It is dry：It is 30% to air-dry or dry in the shade the material fermented to water content, and it is 25 purposes that mesh number is obtained after sieving
Lamb supplement material.
Embodiment 2：
Lamb supplement material is in units of parts by weight, including following raw material：80 parts of sugarcane caudal lobe, 65 parts of peanut straw, fish-bone
80 parts of powder, 50 parts of dregs of beans, 15 parts of compound bacteria, 12 parts of rice washing water, 8 parts of calcium carbonate, 3 parts of amino acid/11, micro- 4 parts, Chinese herbal medicine
10 parts of extract；
The composite bacteria agent obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 0.8g/l, peptone 3.8g/l, glucose 1.0g/l, sodium chloride 0.6g/l, phosphorus
Sour potassium 1.0g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2 is inoculated with：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 0.8ml；
T3：Prepare compound bacteria：The culture medium standing training of Trametes versicolor, microbacterium, saccharomycete will be accessed under 29 DEG C of environment
7d is supported, is during which shaken up manually once every 2d；Compound bacteria is made.
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 7 parts of cordate houttuynia, coptis root 3
Part, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
The Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 3cm, decocts 1 time at a temperature of 80 DEG C, filtering, and filter residue is decocted 2 times with the water of 3 times of parts by weight in 65 DEG C of temperature again,
Filtering, merging filtrate,
D2：By the filtrate that step D1 merges 17 DEG C of temperature, pressure 1.26MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.25 mixed liquor A；
D3：Additional proportion is 0.6 in the mixed liquor A of step D2:1.1 water and the mixed liquid B of ethanol, the mixed liquor
The ratio of A and mixed liquid B is 1.6:0.8；Temperature 50 C, power 40W, frequency 23KHz Ultrasonic Conditions under stir
125min；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, is taken
Supernatant；
D5：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The supernatant of step D4 is spray-dried, and obtains Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned lamb supplement material；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 35
Mesh；The mixed-powder of gained is uniformly mixed with fishbone dust, dregs of beans, calcium carbonate, while sprays and adds rice washing water, mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino acid, micro under the conditions of 29 DEG C, 700r/min
Element, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal in 28 DEG C of temperature, humidity 65%, without air stream
Ferment 6d under conditions of logical；
S3：It is dry：It is 35% to air-dry or dry in the shade the material fermented to water content, and it is 35 purposes that mesh number is obtained after sieving
Lamb supplement material.
Embodiment 3：
Lamb supplement material is in units of parts by weight, including following raw material：90 parts of sugarcane caudal lobe, 70 parts of peanut straw, fish-bone
90 parts of powder, 60 parts of dregs of beans, 20 parts of compound bacteria, 14 parts of rice washing water, 10 parts of calcium carbonate, 4 parts of amino acid/11, micro- 6 parts, medium-height grass
12 parts of medicament extract；
The composite bacteria agent obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 1g/l, peptone 5g/l, glucose 1.2g/l, sodium chloride 0.9g/l, potassium phosphate
1.3g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2 is inoculated with：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 0.5-1.0ml；
T3：Prepare compound bacteria：The culture medium standing of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment
9d is cultivated, is during which shaken up manually once every 2d；Compound bacteria is made.
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：7 parts of wrinkled giant hyssop, 8 parts of cordate houttuynia, coptis root 4
Part, 10 parts of garlic, 4 parts of purple perilla, 10 parts of STEVIA REBAUDIANA, 8 parts of rhizoma zingiberis, 4 parts of Radix Codonopsis, 10 parts of radix glycyrrhizae, 9 parts of jujube；
The Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 3cm, decocts 1 time at a temperature of 90 DEG C, and filtering, filter residue decocts 2 with the water of 3 times of parts by weight at a temperature of 80 DEG C again
It is secondary, filtering, merging filtrate,
D2：By the filtrate that step D1 merges 15 DEG C of temperature, pressure 1.5MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.30 mixed liquor A；
D3：Additional proportion is 0.7 in the mixed liquor A of step D2:1.2 water and the mixed liquid B of ethanol, the mixed liquor
The ratio of A and mixed liquid B is 2:0.8；55 DEG C of temperature, power 45W, frequency 25KHz Ultrasonic Conditions under stir 30min；
Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 40 DEG C, rotating speed is 2400r/min, takes supernatant；
D5：, will under the conditions of speed 1500ml/h, 70 DEG C of feeding temperature, 40 DEG C of drop temperature, atomizing pressure 2.15MPa
The supernatant of step D4 is spray-dried, and obtains Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned lamb supplement material；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 45
Mesh；The mixed-powder of gained is uniformly mixed with fishbone dust, dregs of beans, calcium carbonate, while sprays and adds rice washing water, mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino acid, micro under the conditions of 30 DEG C, 800r/min
Element, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal in 28 DEG C of temperature, humidity 70%, without air stream
Ferment 7d under gating condition；
S3：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, and obtaining mesh number after sieving is
The lamb supplement material of 45 mesh.
Comparative example 1：
Lamb supplement material is in units of parts by weight, including following raw material：80 parts of sugarcane caudal lobe, 65 parts of peanut straw, fish-bone
80 parts of powder, 50 parts of dregs of beans, 15 parts of saccharomycete, 12 parts of rice washing water, 8 parts of calcium carbonate, 3 parts of amino acid/11, micro- 4 parts, Chinese herbal medicine
10 parts of extract；
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 7 parts of cordate houttuynia, coptis root 3
Part, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
The Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 3cm, decocts 1 time at a temperature of 80 DEG C, and filtering, filter residue decocts 2 with the water of 3 times of parts by weight at a temperature of 65 DEG C again
It is secondary, filtering, merging filtrate,
D2：By the filtrate that step D1 merges 17 DEG C of temperature, pressure 1.26MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.25 mixed liquor A；
D3：It is 0.6 that Volume fraction is added in the mixed liquor A of step D2:1.1 water and the mixed liquid B of ethanol, it is described
The Volume fraction of mixed liquor A and mixed liquid B is 1.6:0.8；Temperature 50 C, power 40W, frequency 23KHz ultrasonic wave bar
125min is stirred under part；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, is taken
Supernatant；
D5：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The supernatant of step D4 is spray-dried, and obtains Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned lamb supplement material；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 35
Mesh；The mixed-powder of gained is uniformly mixed with fishbone dust, dregs of beans, calcium carbonate, while sprays and adds rice washing water, mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino acid, micro under the conditions of 29 DEG C, 700r/min
Element, saccharomycete are uniformly mixed, then by uniformly mixed material diaphragm seal in 28 DEG C of temperature, humidity 65%, without air stream
Ferment 6d under gating condition；
S3：It is dry：It is 35% to air-dry or dry in the shade the material fermented to water content, and it is 35 purposes that mesh number is obtained after sieving
Lamb supplement material.
Comparative example 2
The technical solution of comparative example 2 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds
Raw material surface 3cm, decocts 1 time at a temperature of 80 DEG C, and filtering, filter residue decocts 2 with the water of 3 times of parts by weight at a temperature of 65 DEG C again
It is secondary, filtering, merging filtrate；The ethanol solution of the filtrate volume fraction 0.5% is added in the filtrate of merging, is uniformly mixed,
Obtain mixed liquor A；
D2：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The mixed liquor A of step D1 is spray-dried, and obtains Chinese herbal medicine extract.
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 3：
The technical solution of comparative example 3 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：9 parts of STEVIA REBAUDIANA, 7 parts of cordate houttuynia, purple perilla 3
Part, 7 parts of rhizoma zingiberis；
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 4：
The technical solution of comparative example 4 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 3 parts of coptis root, 9 parts of garlic,
3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 5：
Using patent " one kind improves high disease resistance Chinese herbal medicine sheep medicine feed and preparation method thereof " (application number：
201510143866.2) in embodiment 1-5 technical solution prepare sheep medicine feed.
Effect example：
Prepare lamb respectively using the technical solution of embodiment 1-3 and comparative example 1-4 with supplement to be expected, while use comparative example
The technical solution of embodiment 1-5 prepares sheep medicine feed in 5.Selection 90 is 14 ± 1kg lambs (30 days without illness record and weight
Age)；9 groups are divided into, every group 10, wherein 8 groups of feeds prepared using above-mentioned technical proposal carry out nursing experiment, is fed daily
Material once, and is feeding in the morning；Remaining 1 group of lamb only eats breast milk, as blank control example；Experimental period be 40 days, every 10
Its morning weighs once on an empty stomach, takes a blood sample once at the jugular vein of lamb on the day of off-test, blood volume 5ml.Off-test
The palatability of the rate of body weight gain of lamb, sick rate result and supplement material is as shown in table 1 afterwards；Lamb blood middle leukocytes investigation result is such as
Shown in table 2.
Table 1：
Table 2：
It was found from the embodiment 2 of table 1 and comparative example 1-2, if individually using saccharomycetes to make fermentation raw material or Chinese herbal juice not
Concentrated directly extraction, centrifuging and taking supernatant, then the feed palatability that is produced is poor, gaining in weight of lamb unobvious and lamb are easy
Infection disease causes to give birth to；This is because the Trametes versicolor and microbacterium in inventing very much the composite bacteria of use have destroy cell membrane,
The degraded effect such as crude fibre and pectin, and can be broken while fermentation with saccharomycete with the use of synergistic effect can be produced
Bad sugarcane caudal lobe and cell membrane, degraded crude fibre in stalk so that the supplement material prepared, which is easier to be digested by lamb, to be inhaled
Receive.Expect in addition, can be seen that the lamb prepared using technical solution of the present invention from embodiment 2 and comparative example 1-4 and supplement, it is agreeable to the taste
Property it is preferable and full of nutrition, gaining in weight of lamb is obvious after eating, and without sick phenomenon, illustrates Chinese herbal medicine processing using the present invention
Technique, i.e., concentrated active principle by concentrated medicine juice and collected, then extracts supernatant, each technique step through extraction, centrifugal treating
It is rapid closely coupled, it can be very good to come out the active ingredient concentration extraction in Chinese herbal medicine, greatly improve the profit of active principle
With rate；Pass through compound bacteria-fermented at the same time so that the active ingredient and amino acid of Chinese herbal medicine extract, trace element diffusion well
Or penetrate into supplement material, improve the nutritive value of supplement material.
Neutrophil percentage in lamb blood is related to the nospecific immunity of lamb；Within the specific limits,
Neutrophil percentage is got over, then the non-specific immunity of lamb is stronger.It was found from the embodiment 1-3 of table 2, embodiment
The percentage difference unobvious of neutrophil between 1-3, but compared with comparative example 1-5 and blank control example, 3 will be higher by
More than percentage point, illustrate that the percentage of neutrophil in blood can be effectively improved by adding Chinese herbal medicine extract, you can
To effectively improve the non-specific immunity of lamb, sick rate is reduced.In addition, understand the leaves of pulse plants from embodiment 2 and comparative example 3-4
Perfume, cordate houttuynia, coptis root, garlic, purple perilla, STEVIA REBAUDIANA, rhizoma zingiberis, Radix Codonopsis, radix glycyrrhizae, jujube play synergistic effect, wrinkled giant hyssop and coptis root, big
Garlic is used cooperatively, having effects that it is turbid and in, purging intense heat and detonicating, sterilization stomach invigorating, Radix Codonopsis, jujube be used cooperatively have bowl spares benefit
Gas, the effect of strengthen immunity, cordate houttuynia have antibacterial, antiviral, raising immunity of organisms and other effects, and rhizoma zingiberis uses with purple perilla
Exterior cold, pre- avian influenza-prevention can be driven；And STEVIA REBAUDIANA primarily serves seasoning effect, avoid the Chinese herbal medicine that boils excessively bitter, radix glycyrrhizae
Primarily serve the effect of coordinating the drug actions of a prescription so that the ten more rational compatibilities of taste Chinese herbal medicine, it is respective the effect of reach maximization.
The present invention is can be seen that from embodiment 2 and the comparative example 5 of table 1, table 2 to mend using the lamb of high-effective service preparation
The percentage of charge either palatability, gaining in weight of lamb rate, lamb sick rate and lamb neutrophil is better than existing skill
Feed is made in art.In addition, it was found from embodiment 2 and blank control example, the later lamb of 30 ages in days should suitably feed supplement and come
Material, to improve adaptability of the lamb to plant feed, while improves its immunity.
Above content is to combine preferred embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of present inventive concept, it can also make some replacements or modification to the embodiment that these have been described, and these are replaced
Generation or variant should all be considered as belonging to protection scope of the present invention.
Claims (10)
1. high-effective service, it is characterised in that the step such as the high-effective service is prepared by culture medium, is inoculated with, compound bacteria culture
Suddenly it is prepared；
The high-effective service includes following raw material：Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, potassium phosphate, Trametes versicolor,
Microbacterium, saccharomycete.
2. high-effective service as claimed in claim 1, it is characterised in that the Trametes versicolor, microbacterium, the ratio of saccharomycete
For：1:1:0.5.
3. high-effective service as claimed in claim 1, it is characterised in that the Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, chlorination
Sodium, the amount of potassium phosphate are respectively：Sucus Caulis et Folium Brassicae capitatae 0.5g/l-1g/l, peptone 2.5g/l-5g/l, glucose 0.7g/l-1.2g/
L, sodium chloride 0.4g/l-0.9g/l, potassium phosphate 0.8g/l-1.3g/l.
4. high-effective service as claimed in claim 3, it is characterised in that the Sucus Caulis et Folium Brassicae capitatae 0.8g/l, peptone 3.8g/l,
Glucose 1.0g/l, sodium chloride 0.6g/l, potassium phosphate 1.0g/l.
5. high-effective service as claimed in claim 1, it is characterised in that the step specifically includes following：
T1：Prepare culture medium：Sucus Caulis et Folium Brassicae capitatae, peptone, glucose, sodium chloride, potassium phosphate are uniformly mixed, sterilizes, is trained
Support base；
T2：Inoculation：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, are cultivated per 100g
In base, inoculum concentration 0.5-1.0ml；
T3：Compound bacteria culture：The culture medium quiescent culture of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment
6-9d, is made compound bacteria.
6. high-effective service as claimed in claim 5, it is characterised in that every 2d in the step T3 compound bacteria incubations
Shake up once manually.
7. application of the efficient culture medium in lamb supplement material is prepared as described in claim 1-6 is any, it is characterised in that institute
The method for stating preparation lamb supplement material comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, mixed-powder and fishbone dust, dregs of beans, the carbon of gained
Sour calcium is uniformly mixed, while is sprayed and added rice washing water, and mixture D is made；
S2：Fermenting raw materials：By mixture D and Chinese herbal medicine extract, amino acid, micro- under the conditions of 28-30 DEG C, 600-800r/min
Secondary element, compound bacteria are uniformly mixed, then the material diaphragm seal that will be uniformly mixed, in 25-28 DEG C of temperature, humidity 60%-
Ferment 5-7d under the conditions of 70%；
S3：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, and it is 25-45 that mesh number is obtained after sieving
Purpose lamb supplement material.
8. application of the efficient culture medium as claimed in claim 7 in lamb supplement material is prepared, it is characterised in that the step
The addition of S2 compound bacterias is the 5-8% of mixture D mass fractions.
9. application of the efficient culture medium as claimed in claim 7 in lamb supplement material is prepared, it is characterised in that the step
S2 Chinese herbal medicine extracts are in units of parts by weight, including following raw material：It is 5-7 parts of wrinkled giant hyssop, 6-8 parts of cordate houttuynia, 2-4 parts of coptis root, big
8-10 parts of garlic, 2-4 parts of purple perilla, 8-10 parts of STEVIA REBAUDIANA, 6-8 parts of rhizoma zingiberis, 2-4 parts of Radix Codonopsis, 8-10 parts of radix glycyrrhizae, 7-9 parts of jujube.
10. application of the efficient culture medium as claimed in claim 9 in lamb supplement material is prepared, it is characterised in that in described
Herb extracts obtain by the following method：
D1：All raw materials of Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water exceeds raw material
Surface 2-3cm, decocts 1 time at a temperature of 70-90 DEG C, and filtering, filter residue is again with the water of 2-3 times of parts by weight at a temperature of 50-80 DEG C
Decoct 1-2 times, filtering, merging filtrate；
D2：The filtrate that step D1 merges is concentrated under 10-15 DEG C, the cryogenic high pressure environment of pressure 1.02-1.5MPa opposite
Density is the mixed liquor A of 1.20-1.30；
D3：Additional proportion is 0.5-0.7 in the mixed liquor A of step D2:The water of 1.0-1.2 and the mixed liquid B of ethanol, it is described mixed
The ratio for closing liquid A and mixed liquid B is 1-2:0.8；40-55 DEG C of temperature, power 35-45W, frequency 20-25KHz ultrasonic wave bar
20-30min is stirred under part；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, takes supernatant
Liquid；
D5：In speed 1000-1500ml/h, 65-70 DEG C of feeding temperature, 30-40 DEG C of drop temperature, atomizing pressure 1.5-
Under the conditions of 2.15MPa, the supernatant of step D4 is spray-dried, obtains Chinese herbal medicine extract.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527230A (en) * | 2018-12-30 | 2019-03-29 | 广西武鸣绿世界生态农业投资有限公司 | Microorganism formulation, preparation method and its preparing the application in the sheep feed of healthy high-yield Black Hills |
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|---|---|---|---|---|
| CN101485402A (en) * | 2009-02-16 | 2009-07-22 | 北京市农林科学院 | Composite biological feed additive agent for fattening early weaning mutton sheep |
| CN103229900A (en) * | 2013-03-29 | 2013-08-07 | 蚌埠大成食品有限公司 | Weaned lamb forage and preparation method thereof |
| CN104543395A (en) * | 2015-01-06 | 2015-04-29 | 安徽麒麟正大饲料有限公司 | Supplementary lamb feed for promoting growth and enhancing immunity |
| CN106509346A (en) * | 2015-09-10 | 2017-03-22 | 开县钦梅农牧有限公司 | Appetite promoting and spleen strengthening straw feed recipe for young goats |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485402A (en) * | 2009-02-16 | 2009-07-22 | 北京市农林科学院 | Composite biological feed additive agent for fattening early weaning mutton sheep |
| CN103229900A (en) * | 2013-03-29 | 2013-08-07 | 蚌埠大成食品有限公司 | Weaned lamb forage and preparation method thereof |
| CN104543395A (en) * | 2015-01-06 | 2015-04-29 | 安徽麒麟正大饲料有限公司 | Supplementary lamb feed for promoting growth and enhancing immunity |
| CN106509346A (en) * | 2015-09-10 | 2017-03-22 | 开县钦梅农牧有限公司 | Appetite promoting and spleen strengthening straw feed recipe for young goats |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109527230A (en) * | 2018-12-30 | 2019-03-29 | 广西武鸣绿世界生态农业投资有限公司 | Microorganism formulation, preparation method and its preparing the application in the sheep feed of healthy high-yield Black Hills |
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Application publication date: 20180511 |