CN115530372A - Seaweed extract with fat reducing function, preparation method thereof and seaweed extract composition - Google Patents
Seaweed extract with fat reducing function, preparation method thereof and seaweed extract composition Download PDFInfo
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- CN115530372A CN115530372A CN202211224706.7A CN202211224706A CN115530372A CN 115530372 A CN115530372 A CN 115530372A CN 202211224706 A CN202211224706 A CN 202211224706A CN 115530372 A CN115530372 A CN 115530372A
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- seaweed
- seaweed extract
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention belongs to the technical field of health-care food, and particularly relates to a seaweed extract with a fat reducing function, a preparation method thereof and a seaweed extract composition. The invention provides a seaweed extract, the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage content of more than or equal to 1.5 wt%. Fucoxanthin in the seaweed extract belongs to natural carotenoid, is fat-soluble pigment, and can have a remarkable fat-reducing effect by inhibiting the formation of fat cells; meanwhile, the average relative molecular weight of the seaweed extract provided by the invention is 1000-30000 Da, and the seaweed extract is easy to be absorbed by human bodies. The seaweed extract composition provided by the invention has the advantages of effectively increasing fat metabolism, inhibiting adipocyte generation, reducing body fat rate even in a static state of a human body and having obvious weight-losing effect.
Description
Technical Field
The invention belongs to the technical field of health-care food, and particularly relates to a seaweed extract with a fat reducing function, a preparation method thereof and a seaweed extract composition.
Background
In the current society, the pace of life is gradually accelerated, the working pressure of modern people is increased day by day, the working and learning time is too long, the exercise time is reduced, the people sit for a long time, and the people are not moved to cause obesity, constipation and increase the weight and the body fat. In addition, modern people are busy in working, and always eat fast food and take out, the situation of weight and body fat increase is aggravated by unreasonable dietary structure, excessive nutrient intake, unbalanced nutrition, much oil, much salt and high calorie. Obesity is mainly due to an acquired lifestyle discrepancy such as too much food intake, too little exercise, etc. The obesity not only can bring about the change of body form, but also can cause the increase of the incidence of chronic diseases such as hypertension, hyperlipidemia, diabetes, coronary heart disease, malignant tumor and the like, and the obesity is an invisible killer in our life nowadays.
However, the weight-reducing products on the market generally fall into the following categories: 1. promoting excretion and losing weight, promoting intestinal tract peristalsis, and causing excretion in a short time after administration, the method has obvious body feeling and high speed, but can be accompanied with abdominal discomfort and distending pain, can cause damage to intestinal tract after long-term administration, and has no obvious effect on losing weight; 2. meal replacement, which reduces energy intake by the form of meal replacement, has bad taste, is easy to hungry, is not easy to persist, and can cause rebound; 3. weight-reducing medicines have large side effect and damage to the body; 4. exercise and fitness, healthy weight reduction is good for the body, but modern time is limited, and most people cannot insist on the body due to personal reasons and are unwilling to exercise.
Therefore, the current commercial weight-reducing products can not realize effective reduction of body fat in a static state of a human body, and have poor weight-reducing effect.
Disclosure of Invention
The invention aims to provide a seaweed extract with a fat reducing function, a preparation method thereof and a seaweed extract composition, wherein the seaweed extract provided by the invention can inhibit the formation of fat cells and has a remarkable fat reducing effect; the seaweed extract composition provided by the invention can effectively increase fat metabolism and inhibit adipocyte generation, has the effect of reducing the body fat rate even in a static state of a human body, and has an obvious weight-losing effect.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a seaweed extract with a fat-reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage content of more than or equal to 1.5 wt%.
Preferably, the fucoxanthin content of the seaweed extract is 1.5-3 wt%.
The invention provides a preparation method of a seaweed extract with a fat reducing function, which comprises the following steps:
mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and performing solid-liquid separation to obtain seaweed enzymolysis precipitate; the compound hydrolase comprises alginate lyase and cellulase;
dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid;
concentrating the purified seaweed extract solution, and drying to obtain the seaweed extract with the function of reducing fat.
Preferably, the mass of the compound hydrolase is 0.5-1.0 per mill of the fresh weight of the fresh seaweed.
Preferably, the mass ratio of the alginic acid lyase to the cellulase is (1-3) to (1-3); the enzyme activity units of the alginate lyase and the cellulase are independently 5-50 ten thousand U/g.
Preferably, the temperature of the enzymolysis is 35-60 ℃; the heat preservation time of the enzymolysis is 1 to 6 hours; the pH value of the enzymolysis is 5-9.
Preferably, before the mixing, preparing the fresh seaweed into seaweed water slurry, and crushing the seaweed water slurry to obtain seaweed particle slurry; the particle size of the seaweed particles in the seaweed particle slurry is 10-30 mu m.
Preferably, the pulverization is performed by a colloid mill, and the pulverization power of the colloid mill is 37-45W; the crushing speed of the colloid mill is 2000-3000 r/min.
The invention provides a seaweed extract composition which comprises the following components in parts by mass: 1-35 parts of seaweed extract, 1-30 parts of mulberry leaf extract, 1-20 parts of green tea, 1-20 parts of coffee, 1-20 parts of green coffee, 1-10 parts of L-carnitine and 1-15 parts of opuntia ficus-indica; the seaweed extract is the seaweed extract with the fat reducing function in the technical scheme or the seaweed extract with the fat reducing function prepared by the preparation method in the technical scheme.
Preferably, the beverage also comprises auxiliary materials, wherein the auxiliary materials comprise one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food essence, xanthan gum, citric acid, povidone K30 and gelatin.
The invention provides a seaweed extract with a fat reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage content of more than or equal to 1.5 wt%. The fucoxanthin in the seaweed extract provided by the invention has the mass percentage content of more than or equal to 1.5wt%, belongs to natural carotenoid, is fat-soluble pigment, and can have a remarkable fat-reducing effect by inhibiting the formation of fat cells; meanwhile, the average relative molecular weight of the seaweed extract provided by the invention is 1000-30000 Da, and the seaweed extract is easy to be absorbed by human bodies.
The invention provides a preparation method of a seaweed extract with a fat reducing function, which comprises the following steps: mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and performing solid-liquid separation to obtain seaweed enzymolysis precipitate; the compound hydrolase comprises alginate lyase and cellulase; dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and performing anion-cation exchange resin chromatography on leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid; concentrating the purified seaweed extract solution, and drying to obtain the seaweed extract with the function of reducing fat. The preparation method provided by the invention adopts the compound hydrolase comprising alginate lyase and cellulase to carry out enzymolysis reaction on the seaweed, effectively reduces the molecular weight of alginic acid in the seaweed, promotes fucoxanthin in the seaweed to be separated from the seaweed more easily, and then adopts the alcohol solvent to further extract, and because alginic acid obtained simultaneously during the enzymolysis reaction reacts with calcium ions to generate calcium alginate with stable structure, the alginic acid stably exists in the form of calcium alginate when the fucoxanthin is extracted by the alcohol solvent, so that the fucoxanthin with higher purity can be extracted; after fucoxanthin is further enriched in the alcohol solvent, the obtained seaweed extract is purified by anion-cation exchange resin chromatography, so that the fucoxanthin content is high, the molecular weight is moderate, and the seaweed extract is easy to absorb by a human body.
The invention provides a seaweed extract composition which comprises the following components in parts by mass: 1-35 parts of seaweed extract, 1-30 parts of mulberry leaf extract, 1-20 parts of green tea, 1-20 parts of coffee, 1-20 parts of green coffee, 1-10 parts of L-carnitine and 1-15 parts of opuntia ficus-indica; the seaweed extract is the seaweed extract with the fat reducing function in the technical scheme or the seaweed extract with the fat reducing function prepared by the preparation method in the technical scheme. In the invention, the mulberry leaf extract has the functions of reducing blood sugar and blood fat and inhibiting lipogenesis; the tea polyphenols contained in green tea can inhibit fat absorption and fat generation in vivo; caffeine in coffee has an effect of increasing the concentration of fatty acids in blood, and once the concentration of fatty acids in blood increases, fatty acids are absorbed by muscles and consumed in a manner of converting body energy, thereby decomposing fat accumulated in the body; the chlorogenic acid contained in the green coffee can obviously inhibit the activities of fatty acid synthetase, HMG-CoA reductase and fatty coenzyme A, ACT in a fat metabolic pathway, and improve the expression of fatty acid beta-oxidation and peroxisome proliferator activated receptor alpha, so that the lipid metabolism is regulated, and the green coffee has obvious effects of inhibiting fat and promoting fat decomposition; l-carnitine is a key substance in the fat metabolism process, can promote fatty acid to enter mitochondria for oxidative decomposition, is a carrier for transporting fatty acid, and can improve the oxidation rate of fat and reduce the consumption of glycogen; the seaweed extract composition provided by the invention is prepared by compounding the seaweed extract with the mulberry leaf extract, green tea, coffee, green coffee, L-carnitine and opuntia ficus-indica, so that the seaweed extract composition has the remarkable effects of effectively increasing fat metabolism and inhibiting lipogenesis within the range of the mass parts, and reducing the body fat rate even in a static state of a human body.
The seaweed extract composition provided by the invention can be prepared into various dosage forms, is stable in product and is easy for industrial production.
Drawings
FIG. 1 is a liquid chromatogram of a seaweed extract having a fat reducing function prepared in example 1 of the present invention.
Detailed Description
The invention provides a seaweed extract with a fat reducing function, wherein the average relative molecular weight of the seaweed extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage content of more than or equal to 1.5 wt%.
In the present invention, all the preparation starting materials/components are commercially available products well known to those skilled in the art unless otherwise specified.
In the invention, the fucoxanthin content in the seaweed extract is preferably 1.5-3 wt%.
The seaweed extract provided by the invention has the effects of burning fat and promoting fat metabolism, and has an obvious weight losing effect.
The invention provides a preparation method of a seaweed extract with a fat reducing function, which comprises the following steps:
mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and performing solid-liquid separation to obtain seaweed enzymolysis precipitate; the compound hydrolase comprises alginate lyase and cellulase;
dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on leaching filtrate after solid-liquid separation to obtain seaweed extract purified liquid with the fat reducing function;
concentrating the purified seaweed extract solution, and drying to obtain the seaweed extract with the function of reducing fat.
Mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and performing solid-liquid separation (hereinafter referred to as first solid-liquid separation) to obtain seaweed enzymolysis precipitate; the compound hydrolase comprises alginate lyase and cellulase.
In the present invention, the fresh seaweed is particularly preferably fresh kelp and/or fresh undaria pinnatifida.
In the present invention, the solid content of the fresh seaweed is preferably not less than 15% by mass, more preferably not less than 20% by mass.
In a particular embodiment of the invention, the fresh seaweed is preferably purchased from Qingdao Shandong.
In the present invention, the fresh seaweed does not exhibit a large area yellowing or dehydration phenomenon.
The source of the fresh seaweed is not particularly limited in the present invention, and the seaweed can be obtained by using a commercially available product or a self-prepared product which is conventional in the art.
In the invention, before the mixing, the fresh seaweed is prepared into seaweed water slurry, and the seaweed water slurry is crushed to obtain seaweed particle slurry; the particle size of the seaweed particles in the seaweed particle slurry is 10-30 mu m.
In the present invention, the present invention preferably further comprises subjecting the fresh seaweed to crushing prior to said crushing. Obtaining seaweed sheets; preparing the seaweed slices into seaweed water slurry, and crushing the seaweed water slurry. In the invention, the particle size of the seaweed sheet is less than or equal to 20 meshes. The invention has no special requirements on the specific implementation process of the crushing.
In the present invention, the content of the seaweed or seaweed pieces in the fresh seaweed water slurry or the seaweed piece water slurry is preferably 10% to 20% by mass, and more preferably 15% to 17% by mass.
In the invention, the pulverization is colloid mill pulverization, and the pulverization power of the colloid mill is preferably 37-45W, and more preferably 45W.
In the present invention, the rotational speed of the colloid mill is preferably 2000 to 3000r/min.
In the present invention, the particle size of the seaweed particles in the seaweed particle slurry is preferably 10 to 30 μm, more preferably 20 μm.
In the present invention, the water-soluble inorganic calcium salt is particularly preferably calcium chloride.
In the present invention, the mixing of the fresh seaweed, water-soluble inorganic calcium salt and complex hydrolase preferably comprises the steps of: ultrasonically mixing the fresh seaweed, water and water-soluble inorganic calcium salt to obtain feed liquid to be subjected to enzymolysis; and mixing the feed liquid to be subjected to enzymolysis with the compound hydrolase. In the present invention, the time of the ultrasonic treatment is preferably 0.5 to 3 hours.
In the invention, during enzymolysis, the mass of the water-soluble inorganic calcium salt is 1-2 of the fresh weight of the fresh seaweed.
In the invention, the mass of the compound hydrolase is 0.5-1.0 per mill of the fresh weight of the fresh seaweed, and more preferably 0.6-0.8 per mill.
In the invention, the mass ratio of the alginic acid lyase to the cellulase is preferably (1-3) to (1-3), more preferably (1-2) to (1-2).
In the invention, the enzyme activity unit of the alginate lyase is preferably 5-50 ten thousand U/g, more preferably 20 ten thousand U/g or 50 ten thousand U/g.
In the present invention, the alginate lyase is purchased from Baiying biotechnology, inc. in Jiangxi.
In the invention, the enzyme activity unit of the cellulase is preferably 5-50 ten thousand U/g, more preferably 20 ten thousand U/g or 50 ten thousand U/g.
In the present invention, the cellulase is purchased from Novoxil (China) Biotechnology Ltd.
In the present invention, the temperature of the enzymatic hydrolysis is preferably 35 to 60 ℃, more preferably 40 to 50 ℃.
In the present invention, the incubation time for the enzymatic hydrolysis is preferably 1 to 6 hours, and more preferably 2 to 3 hours.
In the present invention, the pH of the enzymatic hydrolysis is preferably 5 to 9, more preferably 6 to 8.
In the invention, an enzymolysis reaction solution is obtained after the enzymolysis reaction, and the first solid-liquid separation is carried out after the enzymolysis reaction solution is subjected to enzyme deactivation treatment preferably in a kettle. In the present invention, the enzyme deactivation treatment is preferably performed in the following manner: heating the enzymolysis reaction solution to a temperature for enzyme deactivation, and carrying out heat preservation and enzyme deactivation; in the invention, the enzyme deactivation temperature is preferably 70-90 ℃, and the heat preservation time of the enzyme deactivation is preferably 30-60 min. In the present invention, the enzyme deactivation is to denature and inactivate various enzymes in the complex hydrolase in the enzymatic reaction solution, thereby terminating the enzymatic reaction.
In the present invention, the first solid-liquid separation embodiment is preferably filtration. In the present invention, the filtration is preferably performed using a filter screen having a mesh size of preferably 20 to 40 mesh, more preferably 20 mesh or 40 mesh. In the invention, the first solid-liquid separation is carried out to obtain the seaweed enzymolysis precipitate.
After the seaweed enzymolysis precipitate is obtained, the seaweed enzymolysis precipitate is dispersed in an alcohol solvent for leaching, and leaching filtrate after solid-liquid separation (hereinafter referred to as second solid-liquid separation) is subjected to anion exchange resin chromatography and cation exchange resin chromatography in turn to obtain a seaweed extract purified solution.
In the present invention, the alcohol solvent is particularly preferably ethanol or an ethanol-water mixed solvent.
In the present invention, the volume percentage content of ethanol in the ethanol-water mixed solvent is preferably 95 to 100%, and is not equal to 100%.
In the invention, the mass ratio of the seaweed enzymatic hydrolysis precipitate to the alcohol solvent is 1:5.
In the present invention, the leaching time is preferably 1 hour.
In the present invention, the leaching is preferably performed under stirring.
In the present invention, the second solid-liquid separation embodiment is preferably filtration. The invention is not particularly limited to the specific embodiments of the concerns described. In the present invention, the filtration is preferably performed using a filter screen having a mesh size of preferably 20 to 40 mesh, more preferably 20 mesh or 40 mesh.
In the present invention, the anion exchange resin chromatography embodiment is preferably: passing the leach filtrate through the anion exchange resin column for chromatography.
In the present invention, the cation exchange resin chromatography embodiment is preferably: and (3) carrying out chromatography on the initial chromatographic solution subjected to the anion exchange resin chromatography by using a cation exchange resin column.
In the present invention, the cation exchange resin is chromatographed to obtain a chromatography liquid, and in the present invention, the chromatography liquid is preferably post-treated to obtain the purified solution of the seaweed extract. In the present invention, the post-treatment preferably comprises: and sequentially filtering the chromatographic solution by a filter column and ultrafiltering by an ultrafiltration column. In the present invention, the size of the micropores of the filtration column is preferably 0.5 to 1 μm; the filter element specification of the ultrafiltration column is preferably 0.02 μm.
After the seaweed extract purified liquid is obtained, the seaweed extract purified liquid is concentrated and dried to obtain the seaweed extract with the fat reducing function.
In the present invention, the concentration is preferably concentration under reduced pressure.
In the present invention, the degree of vacuum for the concentration under reduced pressure is preferably 0.8 to 1.0MPa.
In the present invention, the temperature of the concentration under reduced pressure is preferably 30 to 40 ℃.
In the present invention, the concentration is performed to obtain a concentrated solution, and the mass percentage of the concentrated solution is preferably 15 to 30%, and more preferably 20 to 25%. The present invention preferably dries the concentrate.
In the present invention, the drying is particularly preferably freeze drying.
In the present invention, the temperature of the freeze-drying is preferably-20 to-50 ℃.
In the present invention, the degree of vacuum of the freeze-drying is preferably-0.07 to-0.09 MPa.
The invention provides a seaweed extract composition which comprises the following components in parts by mass: 1-35 parts of seaweed extract, 1-30 parts of mulberry leaf extract, 1-20 parts of green tea, 1-20 parts of coffee, 1-20 parts of green coffee, 1-10 parts of L-carnitine and 1-15 parts of opuntia ficus-indica; the seaweed extract is the seaweed extract with the fat reducing function in the technical scheme or the seaweed extract with the fat reducing function prepared by the preparation method in the technical scheme.
The seaweed extract composition provided by the invention comprises 1-35 parts by mass of the seaweed extract composition, preferably 10-30 parts by mass, and more preferably 13 parts by mass.
In the present invention, the seaweed extract group preferably has a particle size of 0.25mm or less, more preferably 0.18mm or less
The seaweed extract composition provided by the invention comprises 1-30 parts of mulberry leaf extract by mass, preferably 5-20 parts of mulberry leaf extract by mass, and more preferably 10 parts of seaweed extract by mass.
In the present invention, the purity of the mulberry leaf extract is preferably 95 to 99%.
In the present invention, the particle size of the mulberry leaf extract is preferably 0.25mm or less, more preferably 0.18mm or less
The source of the mulberry leaf extract is not particularly limited, and the mulberry leaf extract can be obtained by adopting conventional commercial products in the field, such as Disemann (China) Co., ltd., food grade and 98% purity.
The seaweed extract composition provided by the invention comprises 1-20 parts of green tea, preferably 5-20 parts of green tea, and more preferably 7 parts of seaweed extract by mass.
In the present invention, the green tea is preferably green tea powder, and the particle size of the green tea powder is preferably 0.25mm or less, more preferably 0.18mm or less.
In the present invention, the purity of the green tea is preferably 95 to 99%.
The source of the green tea is not particularly limited in the present invention, and any commercially available product conventionally used in the art, for example, food grade, 98% purity, readily known as food thoroughfare limited.
The seaweed extract composition provided by the invention comprises 1-20 parts of coffee, preferably 5-20 parts of coffee, and more preferably 7 parts of seaweed extract by mass.
In the present invention, the coffee is preferably coffee powder, and the particle size of the coffee powder is preferably 0.25mm or less, and more preferably 0.18mm or less.
In the present invention, the purity of the coffee is preferably 90 to 99%; the source of the coffee is not particularly limited in the present invention, and may be any commercially available product conventionally used in the art, for example, degummed coffee, inc, food grade, purity 95%.
The seaweed extract composition provided by the invention comprises 1-20 parts of green coffee, preferably 5-20 parts of green coffee, and more preferably 7 parts of green coffee by mass.
In the present invention, the green coffee is preferably green coffee powder, and the particle size of the green coffee powder is preferably 0.25mm or less, and more preferably 0.18mm or less.
In the present invention, the purity of the green coffee is preferably 50 to 99%; the source of the green coffee is not particularly limited in the present invention, and the green coffee can be obtained by using a conventional commercial product in the field, for example, guangzhou Liang Biotechnology Ltd, purity 90%.
Based on the mass parts of the seaweed extract, the seaweed extract composition provided by the invention comprises 1-10 parts of L-carnitine, preferably 1-5 parts, and more preferably 3 parts.
In the invention, the L-carnitine is preferably L-carnitine powder, and the grain size of the L-carnitine powder is preferably less than or equal to 0.25mm, and more preferably 0.18mm.
In the invention, the purity of the L-carnitine is preferably 95-99%; the source of the L-carnitine is not particularly limited, and the L-carnitine can be obtained by adopting conventional commercial products in the field, such as L-carnitine powder from Liaoning nutritional science and technology Co.
Based on the mass parts of the seaweed extract, the seaweed extract composition provided by the invention comprises 1-15 parts of opuntia ficus-indica, preferably 1-10 parts, and more preferably 3 parts.
In the invention, the Opuntia ficus-indica is specifically preferably Opuntia ficus-indica powder, and the grain size of the Opuntia ficus-indica powder is preferably less than or equal to 0.25mm, and more preferably 0.18mm.
In the invention, the purity of the Opuntia ficus-indica is preferably 50-99%; the source of the Opuntia ficus-indica is not particularly limited in the present invention, and conventional commercial products in the field can be adopted, for example, fennerot Biotech limited, and the purity of Opuntia ficus-indica powder is 70%.
In the seaweed extract composition provided by the invention, a synergistic effect exists between the seaweed extract group and coffee, green coffee, mulberry leaf extract, green tea, L-carnitine and opuntia ficus-indica. Fucoxanthin in the seaweed extract belongs to natural carotenoid, has obvious fat burning effect, and can inhibit lipogenesis and promote fat metabolism in static state of human body. The method specifically comprises the following steps: fucoxanthin can regulate expression of mitochondrion disassembly protein (UCP 1) in human body in WAT (white fat), and the effect can reduce formation of WAT and achieve fat reducing effect. Caffeine in coffee has the effect of increasing the concentration of fatty acids in blood, which are absorbed by muscles and consumed in a way that converts body energy. That is, fat accumulated in the body is decomposed. The chlorogenic acid contained in the green coffee can obviously inhibit the activities of fatty acid synthetase, HMG-CoA reductase and fatty coenzyme A, ACT in a fat metabolic pathway, and improve the expression of fatty acid beta-oxidation and peroxisome proliferator activated receptor alpha, thereby regulating the lipid metabolism. The tea polyphenols of green tea can inhibit fat absorption and fat generation in vivo. L-carnitine is a key substance in the fat metabolism process, can promote fatty acid to enter mitochondria for oxidative decomposition, and is a carrier for transporting fatty acid.
In the invention, the main mechanism of the seaweed extract composition is coffee, green coffee, mulberry leaf extract, green tea powder and L-carnitine, various components for promoting fat combustion and improving fat metabolism are provided, and simultaneously along with the progress of fat combustion, fucoxanthin can reduce the generation of WAT (white fat) and promote the generation of BAT (brown fat) by regulating the expression of mitochondrion disassembly protein (UCP 1) in WAT (white fat), the proportion of WAT is reduced and the proportion of BAT is increased by the synergistic action of fucoxanthin and the five fat-burning components, thereby achieving the effects of fat burning and fat reduction.
The seaweed extract composition provided by the invention preferably further comprises auxiliary materials.
In the present invention, the adjuvant preferably includes one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food essence, xanthan gum, citric acid, povidone K30, and gelatin.
The type and amount of the auxiliary materials are not particularly limited in the present invention, and are preferably selected and determined according to the specific formulation of the seaweed extract composition.
In the present invention, the seaweed extract composition is preferably in the form of a tablet, granule, capsule, powder or oral liquid.
In the present invention, the amount of the auxiliary material is preferably 10 to 80% by mass of the seaweed extract composition.
In the present invention, when the seaweed extract composition is preferably in the form of a tablet, the excipient is specifically preferably sorbitol, microcrystalline cellulose, a food flavor, and magnesium stearate.
In the invention, when the dosage form of the seaweed extract composition is preferably a tablet, the seaweed extract composition tablet comprises 13% of seaweed extract, 10% of mulberry leaf extract, 7% of green tea powder, 7% of coffee powder, 7% of green coffee powder, 3% of L-carnitine powder, 3% of opuntia ficus-indica powder, 40% of sorbitol, 8% of microcrystalline cellulose, 0.7% of food essence and 1.3% of magnesium stearate in percentage by mass.
In the present invention, when the seaweed extract composition is preferably in the form of powder, the auxiliary materials are specifically sorbitol, microcrystalline cellulose, a food flavor, and magnesium stearate.
In the invention, when the preferable dosage form of the seaweed extract composition is powder, the seaweed extract composition tablet comprises 13% of seaweed extract, 10% of mulberry leaf extract, 7% of green tea powder, 7% of coffee powder, 7% of green coffee powder, 3% of L-carnitine powder, 3% of opuntia ficus-indica powder, 40% of sorbitol, 8% of microcrystalline cellulose, 0.7% of food essence and 1.3% of magnesium stearate in percentage by mass.
In the invention, when the dosage form of the seaweed extract composition is preferably oral liquid, the auxiliary materials are preferably water, fruit powder, xanthan gum, sucralose, citric acid and food essence. The fruit powder is particularly preferably apple powder; the food essence is particularly preferably apple essence; the water is preferably purified water.
In the invention, when the dosage form of the seaweed extract composition is preferably an oral liquid, the seaweed extract composition tablet comprises, by mass percentage, 2.6% of seaweed extract, 2% of mulberry leaf extract, 1.4% of green tea powder, 1.4% of coffee powder, 1.4% of green coffee powder, 0.6% of L-carnitine powder, 0.6% of Opuntia ficus-indica powder, 5% of apple powder, 0.2% of xanthan gum, 0.1% of sucralose, 0.3% of citric acid, 0.1% of apple essence and 84.3% of water.
The invention also provides a preparation method of the seaweed extract composition, which comprises the following steps:
mixing seaweed extract, mulberry leaf extract, green tea, coffee, green coffee, L-carnitine and opuntia ficus-indica to obtain the seaweed extract composition.
In the present invention, before the mixing, the present invention preferably sieves the above raw materials through a 60-80 mesh sieve and collects undersize fractions to obtain sieved raw materials, and then mixes the sieved raw materials to obtain the seaweed extract composition.
The specific operation of sieving is not particularly limited in the invention, and the conventional sieving operation in the field can be adopted.
After the sieved raw materials are obtained, the sieved raw materials are mixed to obtain a seaweed extract composition; the mixing is preferably carried out in a three-dimensional mixer, the rotation speed of the mixing is preferably 10 to 20rpm, and the mixing time is preferably 20 to 40min.
In the present invention, when the seaweed extract composition is preferably in the form of a tablet, the present invention preferably further comprises tabletting the mixed material obtained by mixing after the mixing, and the tabletting is preferably carried out by a tabletting machine.
In the present invention, when the dosage form of the seaweed extract composition is preferably an oral liquid, the mixing is preferably performed in ingredients, and the mixing is particularly preferably: mixing water with the sieved raw materials and related auxiliary materials to obtain compound feed liquid; filtering the compound feed liquid by a filter element type filter; obtaining a filtered feed liquid; sterilizing the filtrate at UHT high temperature; obtaining a sterilized feed liquid; and filling the sterilized feed liquid into oral liquid by an automatic filling machine.
In order to further illustrate the present invention, the following detailed description of the technical solutions provided by the present invention is made with reference to the accompanying drawings and examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Cleaning fresh kelp (purchased from Qingdao Shandong, the specification is that the solid content of the fresh kelp is more than or equal to 20 percent), crushing the kelp to be less than or equal to 20 meshes, and forming a small piece; crushing the water slurry of the crushed seaweed pieces (the percentage content of the seaweed pieces is 15%) by using a colloid mill, wherein the processing power of the colloid mill is 45W, and the rotating speed is 2500r/min; grinding into slurry of seaweed granules with a particle size of 20 μm.
Ultrasonically mixing the slurry of the seaweed particles, water and calcium chloride, and then mixing the mixture with composite hydrolase for enzymolysis, wherein the composite hydrolase is alginate lyase (purchased from Baiying biotechnology limited in Jiangxi, 50 ten thousand U /) and cellulase (purchased from Novoxil (China) biotechnology limited, 50 ten thousand U/g), the mass ratio of the alginate lyase to the cellulase is 2:1, the using amount of the composite hydrolase is 0.8 per thousand of the fresh weight of the fresh kelp, and the mass of the calcium chloride is 1 of the fresh weight of the fresh kelp; controlling the enzymolysis temperature at 45 +/-5 ℃, and carrying out enzymolysis for 2 hours, wherein the pH value of enzymolysis is 6;
after enzymolysis, heating the enzymolysis reaction liquid to 75 ℃ to inactivate enzyme for 40min, so that alginic acid lyase and cellulase in the compound hydrolase are denatured and inactivated, and filtering the inactivated enzymolysis reaction liquid by using a 40-mesh filter screen after the enzymolysis reaction is stopped to obtain seaweed enzymolysis precipitate;
adding ethanol into the seaweed enzymolysis precipitate, wherein the mass of the ethanol is 5 times of that of the seaweed enzymolysis precipitate, and stirring for 1 hour for leaching; filtering the leaching solution by a 40-mesh filter screen, sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on the obtained leaching filtrate, filtering by a filter column (the micropore of the filter column is 0.5-1 mu m), and carrying out ultrafiltration by an ultrafiltration column (the specification of a filter element is 0.02 mu m) to obtain a seaweed extract purified solution containing fucoxanthin;
concentrating the obtained purified liquid of the seaweed extract to 20 mass percent by adopting a reduced pressure distillation method to obtain a concentrated solution;
freeze drying the concentrated solution (freeze drying temperature of-50 deg.C, vacuum degree of-0.09 Mpa), and freeze drying for 48 hr to obtain Sargassum extract.
Detecting the content of the seaweed extract: the seaweed extract obtained in this example was subjected to fucoxanthin content detection using a high performance liquid chromatograph (Shimadzu model SPD-10AVP, shimadzu, japan), and the result is shown in fig. 1, which indicates that the fucoxanthin content obtained by the detection was 3.54%.
Example 2
The preparation method is basically the same as that of example 1, except that: the mass ratio of the alginate lyase to the cellulase is 1:2.
Example 3
The preparation method is basically the same as that of example 1, except that: the mass ratio of the alginate lyase to the cellulase is 1:1.
Example 4
The preparation method is basically the same as that of example 1, except that: the mass ratio of the alginate lyase to the cellulase is 3:1.
Example 5
The preparation method is basically the same as that of example 1, except that: the mass ratio of the alginate lyase to the cellulase is 1:3.
Comparative example 1
The preparation method is basically the same as that of example 1, except that: enzymolysis is carried out only by adopting alginate lyase.
Comparative example 2
The preparation method is basically the same as that of example 1, except that: enzymolysis is carried out only by using cellulase.
The types and amounts of enzymes used in the enzymatic hydrolysis of examples 1 to 5 and comparative examples 1 to 2, and the fucoxanthin contents of the live seaweed extracts are shown in Table 1.
TABLE 1 content of fucoxanthin obtained after enzymolysis in examples 1 to 5 and comparative examples 1 to 2
| Serial number | Enzyme | Adding amount of | Fucoxanthin content% |
| Comparative example 1 | Alginate lyase | 0.8‰ | 0.71 |
| Comparative example 2 | Cellulase enzymes | 0.8‰ | 0.63 |
| Example 2 | Alginate lyase and cellulase 1:2 | 0.8‰ | 1.91 |
| Example 3 | Alginate lyase and cellulase 1:1 | 0.8‰ | 2.21 |
| Example 1 | Alginate lyase and cellulase 2:1 | 0.8‰ | 3.54 |
| Example 4 | Alginate lyase and cellulase 3:1 | 0.8‰ | 2.31 |
| Example 5 | Alginate lyase and cellulase 1:3 | 0.8‰ | 2.19 |
It can be seen from the data in table 1 that the fucoxanthin content of the compound hydrolase obtained by combining the alginate lyase and the cellulase according to different proportions is different, mainly because the two enzymes in different combinations have different enzymolysis effects on alginic acid, but the fucoxanthin content is obviously higher than that of a product subjected to enzymolysis by using one enzyme, because: the invention adopts the compounding of alginate lyase and cellulase, so that the molecular weight of alginic acid can be reduced, fucoxanthin in fresh seaweed can be more easily separated from the seaweed, and the fucoxanthin content which can be extracted in the subsequent ethanol extraction is more, wherein the alginate lyase and the cellulase have the mass ratio of 2:1 for enzymolysis in the embodiment 1, and the fucoxanthin content in the finally extracted seaweed extract is the most.
Example 6
The seaweed extract composition provided in this example included, in mass%, 13% (13 kg) of the seaweed extract obtained in example 1, 10% (10 kg) of the mulberry leaf extract, 7% (7 kg) of green tea powder, 7% (7 kg) of coffee powder, 7% (2 kg) of green coffee powder, 3% (3 kg) of l-carnitine powder, 3% (3 kg) of opuntia ficus indica powder, 40% (40 kg) of sorbitol, 8% (8 kg) of microcrystalline cellulose, 0.7% (0.7 kg) of a food flavor, and 1.3% (1.3 kg) of magnesium stearate.
The preparation method comprises the following steps:
respectively sieving the seaweed extract, the mulberry leaf extract, the green tea powder, the coffee powder, the green coffee powder, the L-carnitine powder and the opuntia ficus-indica powder prepared in the example 1 with a 80-mesh sieve;
weighing the sieved raw materials and related auxiliary materials (the auxiliary materials are sorbitol, microcrystalline cellulose, food essence, magnesium stearate and the like which mainly play roles of improving the mouthfeel and increasing the bonding property, the disintegration property, the smoothness and the like of tablets) according to the formula ratio, and putting the raw materials and the related auxiliary materials into a three-dimensional mixer for mixing, wherein the mixing speed is controlled at 15r/min, and the mixing time is 30min to obtain mixed powder;
the mixed powder is put into a hopper of a tablet machine for tabletting, and is pressed into tablets with 0.8 g/tablet.
Example 7
The seaweed extract composition provided in this example included, in mass%, 13% (13 kg) of the seaweed extract prepared in example 1, 10% (10 kg) of the mulberry leaf extract, 7% (7 kg) of the green tea powder, 7% (7 kg) of the coffee powder, 7% (2 kg) of the green coffee powder, 3% (3 kg) of the l-carnitine powder, 3% (3 kg) of the opuntia ficus lndica powder, 40% (40 kg) of sorbitol, 8% (8 kg) of microcrystalline cellulose, 0.7% (0.7 kg) of a food flavor, and 1.3% (1.3 kg) of magnesium stearate.
The preparation method comprises the following steps:
respectively sieving the seaweed extract, the mulberry leaf extract, the green tea powder, the coffee powder, the green coffee powder, the L-carnitine powder and the opuntia ficus-indica powder prepared in the example 1 with a 80-mesh sieve;
weighing the sieved raw materials and related auxiliary materials according to the formula ratio, and putting the raw materials and the related auxiliary materials into a three-dimensional mixer for mixing, wherein the mixing speed is controlled at 15r/min, and the mixing time is 30min to obtain powder.
Example 8
The seaweed extract composition provided in this example includes, in terms of mass%, 2.6% (2.6 kg) of the seaweed extract prepared in example 1, 2% (2 kg) of the mulberry leaf extract, 1.4% (1.4 kg) of the green tea powder, 1.4% (1.4 kg) of the coffee powder, 1.4% (1.4 kg) of the green coffee powder, 0.6% (0.6 kg) of the l-carnitine powder, 0.6% (0.6 kg) of the opuntia ficus indica powder, and adjuvants: 5 percent of apple powder (5 kg), 0.2 percent of xanthan gum (0.2 kg), 0.1 percent of sucralose (0.1 kg), 0.3 percent of citric acid (0.3 kg), 0.1 percent of apple essence (0.1 kg) and 84.3 percent of water (84.3 kg).
The preparation method comprises the following steps:
respectively sieving the seaweed extract, the mulberry leaf extract, the green tea powder, the coffee powder, the green coffee powder, the L-carnitine powder and the opuntia ficus-indica powder prepared in the example 1 with a 80-mesh sieve;
weighing the sieved raw materials and related auxiliary materials according to the formula ratio, adding purified water into a mixing tank, adding the sieved raw materials and the related auxiliary materials into the mixing tank, and stirring and mixing for 30min to obtain compound feed liquid;
filtering the compound feed liquid by a filter element type filter; obtaining a filtered feed liquid;
sterilizing the filtrate at UHT high temperature; obtaining a sterilized feed liquid;
and filling the sterilized feed liquid into oral liquid by an automatic filling machine.
Test example
The test example is a weight reduction, excretion promotion and fat burning test
Experimental sample
The seaweed extract oral liquid obtained in example 8 was filled into 45 mL/bag packages to prepare a test substance having a seaweed extract composition mass concentration of 0.1 g/mL.
Experimental population
(1) Volunteer enrollment criteria
The age is 25-50 years.
(2) Those with overweight, poor exercise, high body fat rate or constipation, and those with a need for fat reduction;
(3) continuously taking for four weeks, 2 times per day in the morning and evening, 1 bag each time;
(2) Treatment regimens
Blank group: no functional component is added, and only 20 cases of auxiliary materials (5 percent (5 kg) of apple powder, 0.2 percent (0.2 kg) of xanthan gum, 0.1 percent (0.1 kg) of sucralose, 0.3 percent (0.3 kg) of citric acid, 0.1 percent (0.1 kg) of apple essence and 84.3 percent (94.3 kg) of water) are added.
Test group: the seaweed extract oral liquid is taken in 45 mL/bag in 1 bag each time, and is taken in 1 bag in the morning and evening respectively, 20 cases in total.
(3) Application method
The two groups are administered orally, 2 bags per day. The composition is administered in 1 bag each time, 1 bag in the morning and evening, and is administered continuously for 4 weeks.
(4) The test results are shown in tables 2 and 3.
TABLE 2 comparison of mean body weight (kg) of two groups of volunteers before the test, 2 weeks and 4 weeks after administration
TABLE 3 comparison of average body fat percentage (%) between 2 and 4 weeks after administration before and after the test in two groups of volunteers
The results of the test examples show that the test groups have obvious effects of reducing weight, promoting defecation and reducing fat compared with the blank group, and that after 2 weeks and 4 weeks of use, the test subjects of the test groups have obvious weight loss and reduced body fat rate after 2 weeks and 4 weeks of use. Therefore, the seaweed extract composition provided by the invention can play a role in obviously burning fat and promoting fat metabolism; the seaweed extract composition has effects of increasing fat metabolism, inhibiting lipogenesis, and reducing body fat rate
Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and all of the embodiments belong to the protection scope of the present invention.
Claims (10)
1. An alga extract having a fat-reducing function, characterized in that the average relative molecular weight of the alga extract is 1000-30000 Da; the seaweed extract contains fucoxanthin with the mass percentage content of more than or equal to 1.5 wt%.
2. The seaweed extract according to claim 1, wherein the fucoxanthin content of the seaweed extract is 1.5-3 wt% based on the mass percentage of fucoxanthin.
3. The method for preparing the seaweed extract having a fat reducing function as set forth in claim 1 or 2, comprising the steps of:
mixing fresh seaweed, water-soluble inorganic calcium salt and compound hydrolase for enzymolysis, and performing solid-liquid separation to obtain seaweed enzymolysis precipitate; the compound hydrolase comprises alginate lyase and cellulase;
dispersing the seaweed enzymolysis precipitate in an alcohol solvent for leaching, and sequentially carrying out anion exchange resin chromatography and cation exchange resin chromatography on the leaching filtrate after solid-liquid separation to obtain a seaweed extract purified solution;
concentrating the purified seaweed extract solution, and drying to obtain the seaweed extract with the function of reducing fat.
4. The preparation method according to claim 3, wherein the mass of the compound hydrolase is 0.5-1.0 per mill of the fresh weight of the fresh seaweed.
5. The method according to claim 3 or 4, wherein the mass ratio of the alginate lyase to the cellulase is (1-3) to (1-3); the enzyme activity units of the alginate lyase and the cellulase are independently 5-50 ten thousand U/g.
6. The preparation method according to claim 3, wherein the temperature of the enzymolysis is 35-60 ℃; the heat preservation time of the enzymolysis is 1 to 6 hours; the pH value of the enzymolysis is 5-9.
7. The method according to claim 3, further comprising, before said mixing, preparing said fresh seaweed into a seaweed aqueous slurry, and pulverizing said seaweed aqueous slurry to obtain a seaweed particle slurry; the particle size of the seaweed particles in the seaweed particle slurry is 10-30 mu m.
8. The preparation method according to claim 7, wherein the pulverization is colloid mill pulverization, and the pulverization power of the colloid mill is 37-45W; the crushing speed of the colloid mill is 2000-3000 r/min.
9. The seaweed extract composition is characterized by comprising the following components in parts by mass: 1-35 parts of seaweed extract, 1-30 parts of mulberry leaf extract, 1-20 parts of green tea, 1-20 parts of coffee, 1-20 parts of green coffee, 1-10 parts of L-carnitine and 1-15 parts of opuntia ficus-indica; the seaweed extract is the seaweed extract with the fat-reducing function of claim 1 or 2 or the seaweed extract with the fat-reducing function prepared by the preparation method of any one of claims 3 to 8.
10. The seaweed extract composition of claim 9, further comprising an adjuvant comprising one or more of water, sorbitol, erythritol, lactose, microcrystalline cellulose, maltodextrin, magnesium stearate, silicon dioxide, titanium dioxide, sucralose, fruit powder, fruit juice, food flavor, xanthan gum, citric acid, povidone K30, and gelatin.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011500556A (en) * | 2007-10-10 | 2011-01-06 | アミコゲン、インク | Composition for prevention or treatment of lipid metabolic disease containing fucoxanthin or seaweed extract containing the same |
| CN102336725A (en) * | 2011-06-10 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Method for extracting fucoxanthine-containing concentrate, product obtained through method, and application of product |
| CN102334688A (en) * | 2011-07-21 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Health-care food with function of reducing fat |
| CN110327325A (en) * | 2019-07-29 | 2019-10-15 | 中国科学院海洋研究所 | A kind of fucoxanthin is repairing the application in injury of kidney caused by cadmium poisoning |
| CN112679450A (en) * | 2020-12-30 | 2021-04-20 | 上海珈凯生物科技有限公司 | Method for comprehensively extracting fucoxanthin and algal polysaccharides from Fucus vesiculosus |
| CN112679452A (en) * | 2020-12-31 | 2021-04-20 | 福建师范大学 | Method for extracting fucoxanthin from kelp |
| CN115039878A (en) * | 2021-03-09 | 2022-09-13 | 仙乐健康科技股份有限公司 | Composition for regulating fat metabolism function |
-
2022
- 2022-10-09 CN CN202211224706.7A patent/CN115530372B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011500556A (en) * | 2007-10-10 | 2011-01-06 | アミコゲン、インク | Composition for prevention or treatment of lipid metabolic disease containing fucoxanthin or seaweed extract containing the same |
| CN102336725A (en) * | 2011-06-10 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Method for extracting fucoxanthine-containing concentrate, product obtained through method, and application of product |
| CN102334688A (en) * | 2011-07-21 | 2012-02-01 | 秦皇岛大惠生物技术有限公司 | Health-care food with function of reducing fat |
| CN110327325A (en) * | 2019-07-29 | 2019-10-15 | 中国科学院海洋研究所 | A kind of fucoxanthin is repairing the application in injury of kidney caused by cadmium poisoning |
| CN112679450A (en) * | 2020-12-30 | 2021-04-20 | 上海珈凯生物科技有限公司 | Method for comprehensively extracting fucoxanthin and algal polysaccharides from Fucus vesiculosus |
| CN112679452A (en) * | 2020-12-31 | 2021-04-20 | 福建师范大学 | Method for extracting fucoxanthin from kelp |
| CN115039878A (en) * | 2021-03-09 | 2022-09-13 | 仙乐健康科技股份有限公司 | Composition for regulating fat metabolism function |
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