CN111034837A - β - (1-3), (1-4) -glucan coffee capable of reducing blood fat and preparation method thereof - Google Patents
β - (1-3), (1-4) -glucan coffee capable of reducing blood fat and preparation method thereof Download PDFInfo
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- CN111034837A CN111034837A CN201911184720.7A CN201911184720A CN111034837A CN 111034837 A CN111034837 A CN 111034837A CN 201911184720 A CN201911184720 A CN 201911184720A CN 111034837 A CN111034837 A CN 111034837A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
- A23F5/36—Further treatment of dried coffee extract; Preparations produced thereby, e.g. instant coffee
- A23F5/40—Further treatment of dried coffee extract; Preparations produced thereby, e.g. instant coffee using organic additives, e.g. milk, sugar
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
- A23F5/36—Further treatment of dried coffee extract; Preparations produced thereby, e.g. instant coffee
- A23F5/38—Agglomerating, flaking or tabletting or granulating
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Abstract
A β - (1-3), (1-4) -glucan hypolipidemic coffee and its preparation method, using wheat bran as raw materials, drying and pulverizing, leaching with hot water, mixing β - (1-3), (1-4) -glucan, rose hip polysaccharide, phellinus igniarius polysaccharide, coffee extract, then granulating, β - (1-3), (1-4) -glucan hypolipidemic coffee obtained by the method has good taste, wherein the added rose hip polysaccharide and phellinus igniarius polysaccharide enhance the hypolipidemic function of β - (1-3), (1-4) -glucan, β - (1-3), (1-4) -glucan hypolipidemic instant coffee of the invention can assist in reducing blood fat, the serviceability is wide, the result is good.
Description
Technical Field
The invention belongs to the technical field of food, and particularly relates to β - (1-3), (1-4) -glucan blood fat reducing coffee and a preparation method thereof.
Background
Wheat bran, flakes or powder, wheat flour by-products. The wheat bran has partial plumule at the end part, accounting for 5-10% of the total amount of wheat bran, 6 layers of wheat bran, and 5 outer layers containing more crude fiber. Wheat bran contains a large amount of vitamin B and cellulose, and is mainly used as an edible raw material, a feed raw material and the like. The dietary fiber is a necessary nutrient element for human body, can quickly improve the constipation condition, can also promote the excretion of fat, and has great prevention and treatment effect on fiber-deficient diseases; the high fiber component is taken in, so that the discharge of steroid in excrement can be reduced, and the main boundary metabolic process of cholesterol in a human body is through the excretion of excrement, so that serum cholesterol can be reduced, and the atherosclerosis of the human body can be slowed down; on the other very important side, dietary fiber in cereals helps to prevent colorectal and rectal cancer; the vitamins B contained in the wheat bran play many functions in the body and are also indispensable nutrient components in the normal metabolism of food. The wheat bran can be used as animal feed, and has high nutritive value.
The wheat bran is rich in glucan, β - (1-3), (1-4) -glucan active structure is polysaccharide consisting of glucose units, the glucan can well activate macrophages and the like, improve the content of leukocytin, cytokinin and special antibodies, and stimulate the immune system of the organism, β - (1-3), (1-4) -glucan is recognized as a very safe substance by the FDA in the United states and can be added into general food, in addition, the glucan can well remove free radicals, resist radiation and dissolve cholesterol, β - (1-3), (1-4) -glucan can be widely used in the industries of medicine, food and the like, the wheat bran is rich in a large amount of glucan, common food can be well enriched in nutrition, the β - (1-3), (1-4) -glucan market has large potential, and the product increment range is high.
Disclosure of Invention
The invention provides β - (1-3), (1-4) -glucan blood fat reducing coffee and a preparation method thereof, wherein a wheat aleurone layer is prepared into soluble β -glucan instant coffee, and the soluble β -glucan instant coffee is brewed and drunk to achieve the effect of assisting in reducing blood fat.
The technical proposal is that β - (1-3), (1-4) -glucan blood fat reducing coffee comprises the following components in parts by weightThe following components: molecular weight MWβ - (1-3), (1-4) -glucan of 220kDa 50-55 parts, molecular weight MW3-4 parts of 250kDa rose hip polysaccharide and MW5-6 parts of 350kDa phellinus igniarius polysaccharide and 60-65 parts of coffee extract.
Preferably, the composition comprises β - (1-3), (1-4) -glucan 55 parts, rose hip polysaccharide 3 parts, phellinus igniarius polysaccharide 6 parts and coffee extract 65 parts in parts by weight.
The preparation method of the blood fat reducing coffee comprises the steps of uniformly mixing β - (1-3), (1-4) -glucan, rose hip polysaccharide, phellinus igniarius polysaccharide and coffee extract, and then granulating.
β - (1-3) and 1-4) -glucan are prepared by collecting wheat bran powder with a water content of less than 5 wt%, pulverizing the bran powder with an air-cooled pulverizer, sieving the pulverized bran particles to obtain fine particles with an average particle size of 150 μm, pulverizing the fine particles with supersonic gas of a flat ceramic liner, subjecting the pulverized particles to supersonic gas milling with an air flow pressure of 8-9 atm and an average particle size of 1 μm, subjecting the pulverized fine particles to ultrasonic microwave extraction with water of 105 ℃ for 20-21 min at a liquid-to-material ratio of 25mL to 1g, an ultrasonic power of 210W and a microwave power of 105W, adding 1wt.% of arabinoxylanase for enzymolysis of arabinoxylan for 50-60 min, inactivating the arabinoxylan at 100 ℃, adding 95% ethanol in an amount 4 times the volume of the product, filtering, precipitating with ethanol, and vacuum drying the precipitate to obtain β - (1-3) and a purity of 1-4) -glucan of ≧ 90%.
The preparation steps of the rose hip polysaccharide are as follows: putting the rose hips into a low-temperature mechanical pulverizer, pulverizing to obtain particles of 20 meshes, extracting the rose hips with distilled water at the temperature of 4 ℃ for 25 minutes at the water content of less than 9 wt%, wherein the liquid-material ratio is 16mL:1g, the ultrasonic power is 150W, the microwave power is 105W, and spray drying to obtain the rose hips polysaccharide.
The preparation method of the phellinus igniarius polysaccharide comprises the following steps: placing phellinus igniarius into a low-temperature mechanical pulverizer, pulverizing to obtain particles of 40 meshes, extracting the mixture for 16-17 minutes by using water of 90 ℃ at the pulverizing temperature of 20 ℃ and the moisture content of less than 9wt.%, wherein the liquid-material ratio is 20mL:1g, the ultrasonic power is 150W, the microwave power is 105W, and vacuum concentrating and drying to obtain phellinus igniarius polysaccharide.
The preparation steps of the coffee extract are as follows: extracting Yunnan coffee with 60-65 deg.C water for 5-6 min at a liquid-material ratio of 12mL:1g, and spray drying to obtain extract.
The β - (1-3), (1-4) -glucan blood fat reducing coffee prepared by the method has good taste, has aromatic flavor of instant coffee, can effectively assist in reducing blood fat, is safe and free of side effects, is suitable for wide people to be used, and is used for researching the effect of the wheat aleurone layer blood fat reducing instant coffee on reducing blood fat of rats.
Detailed description of the invention
The present invention will be better understood from the following detailed description of specific examples, which should not be construed as limiting the scope of the present invention.
Example 1
β - (1-3), (1-4) -glucan blood fat reducing coffee is prepared by taking wheat bran powder with the water content of less than 5wt.%, pulverizing bran powder by using a DCW series air-cooled pulverizer, preferably Xingda morning mechanical science and technology limited company, the pulverized bran particles are sieved to obtain fine particles with the average particle size of 150 microns, pulverizing the fine particles by using a DCA series flat ceramic liner supersonic gas, the air flow pressure is 8 atm, the pulverized particles have the average particle size of 1 micron, performing ultrasonic microwave extraction on the pulverized fine particles by using 105 ℃ water for 20 minutes, the liquid-material ratio is 25mL:1g, the ultrasonic power is 210W, the microwave power is 105W, adding 1% (mass percentage) of arabinoxylan for 50 minutes, performing inactivation at 100 ℃, adding 95% ethanol with the volume of the product obtained in the last step for 4 times, performing alcohol precipitation on the obtained by using ethanol with the concentration ratio of 95% of the volume of the obtained product obtained in the ultrasonic extraction process of 635 mL, the ultrasonic extraction is 7-3W series flat ceramic liner supersonic extraction, the microwave extraction of the microwave extraction, the polysaccharide is 1-4% of the polysaccharide, the polysaccharide obtained by using a concentrated solution obtained by using 7-6-mesh ethanol precipitation, the pulverized polysaccharide obtained by using a distilled water extraction process, the pulverized coffee extract obtained by repeating the process, the process of vacuum drying process, the polysaccharide obtained by using a distilled water extraction process, the pulverized coffee extract of 150-6-mesh distillation process, the pulverized coffee extract is the pulverized coffee extract, the pulverized coffee extract is the pulverized coffee is obtained by using the pulverized coffee extract, the pulverized coffee extract of the pulverized coffee extract, the pulverized coffee extract of the pulverized coffee extract, the pulverized coffee is obtained by using the pulverized coffee powder is obtained by using the pulverized coffee extract, the pulverized coffee powder, the pulverized coffee extract, the.
Example 2
β - (1-3), (1-4) -glucan blood fat reducing coffee is prepared by taking wheat bran powder with the water content of less than 5wt.%, pulverizing bran powder by using a DCW series air-cooled pulverizer, preferably Xingda morning mechanical science and technology limited company, the pulverized bran particles are sieved to obtain fine particles with the average particle size of 150 microns, pulverizing fine particles by using a DCA series flat ceramic inner container supersonic gas, preferably Xingda morning mechanical science and technology limited company, the airflow pressure is 8 atm, the average particle size of the pulverized particles is 1 micron, performing ultrasonic microwave extraction on the pulverized fine particles with 105 ℃ water for 20 minutes, the liquid-material ratio is 25mL:1g, the ultrasonic power is 210W, the microwave power is 105W, 1% (mass percentage) arabinoxylan is added for 60 minutes, performing inactivation at 100 ℃, performing 95% ethanol precipitation on the obtained product in the 4 volume by using 105 ℃ water, performing alcohol precipitation on 6-layer filtration gauze, repeating the precipitation process for 4 times, performing vacuum drying 3660- (1-3) -6- (1: 4) polysaccharides, performing ultrasonic spray extraction on the pulverized polysaccharides in a distilled water-distilled liquid, the pulverized polysaccharides obtained by using a distilled liquid, the pulverized polysaccharides obtained by using a microwave distillation process, the pulverized polysaccharides obtained by using a 90-7-60-mesh pulverizer, the pulverized phellinum polysaccharide-60-mesh-60-mesh-60-mesh-60-mesh-60-mesh-6-mesh-powder-.
Example 3
β - (1-3), (1-4) -glucan blood fat reducing coffee is prepared by taking wheat bran powder with the water content of less than 5wt.%, pulverizing bran powder by using a DCW series air-cooled pulverizer, preferably Xingda morning mechanical science and technology limited company, the pulverized bran particles are sieved to obtain fine particles with the average particle size of 150 microns, pulverizing fine particles by using a DCA series flat ceramic inner container supersonic gas, preferably Xingda morning mechanical science and technology limited company, the airflow pressure is 9 atmospheres, the average particle size of the pulverized particles is 1 micron, performing ultrasonic microwave extraction on the pulverized fine particles with 105 ℃ water for 21 minutes, performing ultrasonic microwave extraction on the fine particles with the liquid-material ratio of 25mL:1g, the ultrasonic power of 210W and the microwave power of 105W, adding 1% (by mass%) arabinoxylan for 60 minutes, performing inactivation on the pulverized particles at 100 ℃, performing alcohol precipitation on the obtained product with 95% ethanol with the volume of 4 times of the powder with the water content of 105 ℃ and the liquid-powder with the liquid-material ratio of 10mL, performing ultrasonic microwave extraction on the ultrasonic microwave power of 10W and the microwave power of 10W for 10W, performing ultrasonic microwave extraction on the microwave power of 105W, performing alcohol precipitation on the pulverized particles with the ethanol precipitation on the pulverized particles with the ethanol extract of 95% of 6-6 layers, repeating the precipitation filtration process, performing the precipitation process, performing vacuum drying on the precipitation process for 4 times, the precipitation process, performing vacuum drying the precipitation process, performing vacuum drying of precipitation process, the precipitation process for 60 minutes, performing vacuum drying of precipitation process for 60 minutes, the precipitation process for 60 minutes, performing vacuum drying of the precipitation process for 6-6 precipitation process for 7-6 precipitation process, the precipitation process for 7-6.
The research results in Table 1 show that β - (1-3), (1-4) -glucan and instant coffee extract have obvious synergistic effect of reducing blood fat, the control group 1 has a weaker blood fat reducing effect on mice when samples with wheat aleurone layer extract and without coffee extract are used, and the control group 2 has a stronger blood fat reducing effect when samples with Pu' er coffee extract and without wheat aleurone layer extract are used, and the wheat aleurone layer extract and coffee extract are combined together.
TABLE 1 dynamic changes (x. + -. s, mg/L) in serum TC in rats of each group
TABLE 2 dynamic changes (x. + -. s, mg/L) in serum TG of rats in each group
Claims (7)
1.β - (1-3), (1-4) -glucan blood fat reducing coffee is characterized by comprising the following components in parts by weight, wherein the molecular weight M isWβ - (1-3), (1-4) -glucan of 220kDa 50-55 parts, molecular weight MW3-4 parts of 250kDa rose hip polysaccharide and MW5-6 parts of 350kDa phellinus igniarius polysaccharide and 60-65 parts of coffee extract.
2. The blood fat reducing coffee according to claim 1, which is characterized by comprising β - (1-3), (1-4) -glucan 55 parts by weight, rose hip polysaccharide 3 parts by weight, phellinus igniarius polysaccharide 6 parts by weight and coffee extract 65 parts by weight.
3. The preparation method of the blood lipid reducing coffee according to claim 1, wherein the steps are mixing β - (1-3), (1-4) -glucan, rose hip polysaccharide, phellinus linteus polysaccharide and coffee extract uniformly, and then granulating.
4. The preparation method of the blood fat reducing coffee according to claim 3, wherein β - (1-3) and 1-4) -glucan are prepared by taking wheat bran powder with a water content of less than 5 wt%, crushing the bran powder by using an air-cooled crusher, sieving the crushed bran particles to obtain fine particles with an average particle size of 150 microns, crushing the fine particles by using supersonic gas with a flat ceramic liner, wherein the gas flow pressure is 8-9 atm, the average particle size of the crushed particles is 1 micron, performing ultrasonic microwave extraction on the crushed fine particles for 20-21 minutes by using water with a temperature of 105 ℃ and ultrasonic waves, the liquid-material ratio is 25mL:1g, the ultrasonic power is 210W, the microwave power is 105W, adding 1wt.% of arabinoxylan for enzymolysis for 50-60 minutes, performing inactivation at 100 ℃, adding 95% ethanol with a volume which is 4 times that of the volume of the obtained product in the previous step, performing ethanol precipitation, filtering, and performing vacuum drying on the obtained precipitate to obtain β - (1-3) and the product with a purity of not less than 90%.
5. The preparation method of the blood fat reducing coffee according to claim 3, wherein the preparation steps of the rose hip polysaccharide are as follows: putting the rose hips into a low-temperature mechanical pulverizer, pulverizing to obtain particles of 20 meshes, extracting the rose hips with distilled water at the temperature of 4 ℃ for 25 minutes at the water content of less than 9 wt%, wherein the liquid-material ratio is 16mL:1g, the ultrasonic power is 150W, the microwave power is 105W, and spray drying to obtain the rose hips polysaccharide.
6. The preparation method of the blood fat reducing coffee according to claim 3, wherein the preparation steps of the phellinus igniarius polysaccharides are as follows: placing phellinus igniarius into a low-temperature mechanical pulverizer, pulverizing to obtain particles of 40 meshes, extracting the mixture for 16-17 minutes by using water of 90 ℃ at the pulverizing temperature of 20 ℃ and the moisture content of less than 9wt.%, wherein the liquid-material ratio is 20mL:1g, the ultrasonic power is 150W, the microwave power is 105W, and vacuum concentrating and drying to obtain phellinus igniarius polysaccharide.
7. The preparation method of the blood fat reducing coffee as claimed in claim 3, wherein the preparation steps of the coffee extract are as follows: extracting Yunnan coffee with 60-65 deg.C water for 5-6 min at a liquid-material ratio of 12mL:1g, and spray drying to obtain extract.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111387326A (en) * | 2020-05-10 | 2020-07-10 | 临清先锋农产品专业合作社 | Formula and production process of phellinus igniarius coffee |
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Cited By (1)
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| CN111387326A (en) * | 2020-05-10 | 2020-07-10 | 临清先锋农产品专业合作社 | Formula and production process of phellinus igniarius coffee |
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