CN112826769A

CN112826769A - A facial mask with effects of improving facial flora, relieving, repairing and keeping moisture

Info

Publication number: CN112826769A
Application number: CN201911159623.2A
Authority: CN
Inventors: 梁活玲; 张目; 李继德
Original assignee: Guangzhou Yuezhuang Biotechnology Co ltd
Current assignee: Guangzhou Yuezhuang Biotechnology Co ltd
Priority date: 2019-11-22
Filing date: 2019-11-22
Publication date: 2021-05-25
Anticipated expiration: 2039-11-22
Also published as: CN112826769B

Abstract

The invention provides a facial mask with the effects of improving facial flora conditions, relieving, repairing and moisturizing, which is prepared by compounding an effective composition of soybean milk lactobacillus fermentation product filtrate, ceramide 3 and centella asiatica extract, has synergistic effects in relieving and moisturizing, reduces main pathogenic bacteria causing skin infection, balances facial resident bacteria and dermatophytes, balances skin microorganisms, effectively reduces skin inflammation, reduces skin redness, increases skin collagen and improves skin moisture.

Description

A facial mask with effects of improving facial flora, relieving, repairing and keeping moisture

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a facial mask with the effects of improving the condition of facial flora, relieving, repairing and moisturizing.

Background

The skin is a natural barrier for preventing the human body from contacting with the outside, and the human body is protected from the invasion of external harmful substances. The skin surface has a large amount of microorganisms which can be divided into resident microorganisms and temporary microorganisms, wherein the resident microorganisms mainly comprise staphylococcus, propionibacterium, micrococcus and the like; the transient flora is obtained by contacting with the outside, and comprises pseudomonas alcaligenes, staphylococcus aureus, enterococcus, streptococcus hemolyticus and the like. Transient flora is the main pathogen causing skin infections.

The microbial flora of normal skin is in a relatively balanced state, and factors influencing the microbial flora of the skin are various, such as the population, the living environment, the air quality, the face care condition and the like. Many times, factors such as modern life style, environmental pollution, improper use of skin care products and the like cause a certain temporary bacterium to be greatly increased, the balance of the microbial flora of the skin is damaged, and adverse conditions such as acne, allergy, erythema and the like appear on the skin.

Disclosure of Invention

The invention aims to provide a facial mask which has the effects of improving the condition of facial flora, relieving, repairing and moisturizing.

In the present invention, the lactobacillus Sojae Atricolor fermented product filtrate is preferably black bean milk lactobacillus fermented product filtrate. The filtrate of the lactobacillus fermentation product of the soybean milk contains active substances such as protein, amino acid, organic acid, polypeptide, vitamin, flavone, hydroxy acid, mineral substances and the like, and has the effects of improving the condition of facial flora, relieving, repairing and moisturizing. It will be understood by those skilled in the art that the lactobacillus fermentive filtrate of the present invention may be prepared by conventional methods in the art or purchased directly, such as optimelalth (cetimin, AXIALYS INNOVATIONS, france).

Ceramide is part of the stratum corneum lipid of the skin, and the ceramide content in the skin can decrease, resulting in water loss, loss of elasticity, and susceptibility to inflammation. Ceramide 3 has effects of repairing skin barrier and keeping moisture. In the present invention, ceramide 3 is preferably liposome-encapsulated ceramide 3. It will be understood by those skilled in the art that the ceramide 3 of the present invention may be prepared by a conventional method in the art or purchased directly, for example, DS-CERASOME1.0 (DOOSAN, korea).

The centella asiatica extract contains various effective components such as asiaticoside, asiatic acid, madecassic acid and the like, can stimulate the proliferation of fibroblasts, increase the synthesis of collagen and fibronectin, and is a blocker for inducing apoptosis, and has obvious biological effect on resisting apoptosis inducer on the histiocytes by reducing the concentration of oxygen free radicals in the histiocytes. The centella asiatica extract can act on tissue cells, reduce local inflammatory reaction and local myofibroblast production, obviously increase the synthesis of extracellular matrix components including collagen in the healing remodeling process, and finally accelerate the epithelialization process of skin wound surfaces. It will be understood by those skilled in the art that the centella asiatica extract of the present invention may be prepared by conventional methods in the art or purchased directly.

According to one aspect of the present invention, there is provided a mask composition comprising a filtrate of lactobacillus fermentation product of soybean milk, ceramide 3 and centella asiatica extract. The filtrate of the lactobacillus fermentation product of the soybean milk is preferably filtrate of the lactobacillus fermentation product of the black soybean milk, and is further preferably optimelatal (oxitin); the ceramide 3 is preferably liposome-encapsulated ceramide 3, and more preferably DS-CERASOME 1.0.

Preferably, the weight percentage of the lactobacillus fermented product filtrate of the soybean milk, preferably the lactobacillus fermented product filtrate of the black soybean milk, and further preferably optimelath (oxitamine) in the mask composition is 0.5-5.0%, preferably 1.0-2.0%; ceramide 3 preferably liposome-encapsulated ceramide 3 further preferably DS-CERASOME1.0, in an amount of 0.2-2.0% by weight, preferably 0.5-1.0% by weight; the centella asiatica extract is present in an amount of 0.2-2.0 wt%, preferably 0.5-1.0 wt%.

Preferably, other skin care actives may optionally be included in the mask compositions of the present invention, such as moisturizers, emollients, soothing anti-inflammatory agents, and the like. Preferably, the weight percentage of the other skin care active ingredients is 9.0-40.0%. Preferably, the humectant is 5-22 wt%, the emollient is 3.0-17.0 wt%, and the soothing anti-inflammatory agent is 0.01-1.0 wt%.

It will be appreciated by those skilled in the art that various other skin care actives commonly used in the art, such as moisturizers, emollients, soothing anti-inflammatory agents, and the like, can be used in the present invention. Preferably, the humectant is one or a mixture of two or more of glycerol, trehalose, allantoin, sodium hyaluronate and butanediol; the emollient is selected from one or two or more of PPG-10 methyl glucose ether, N-602, PEG/PPG-17/6 copolymer, and glycerol glucoside; soothing anti-inflammatory agents such as horse chestnut (AESCULUS CHINENSIS) extract and the like.

Preferably, the mask composition of the present invention may further comprise adjuvants such as thickening agents, chelating agents, preservatives, solubilizers, fragrances, solvents, and the like. Preferably, the thickener is present in an amount of 0.1 to 1.0% by weight, the chelating agent is present in an amount of 0.01 to 1.0% by weight, the preservative is present in an amount of 0.5 to 1.5% by weight, the solubilizer is present in an amount of 0.001 to 0.1% by weight, and the fragrance is present in an amount of 0.0001 to 0.001% by weight.

It will be understood by those skilled in the art that various adjuvants commonly used in the art, such as thickeners, chelating agents, preservatives, solubilizers, fragrances, and solvents, can be used in the present invention. Preferably, the thickener is selected from one or a mixture of two or more of sodium polyacrylate grafted starch, polyacrylate cross-linked polymer-6, and hydrolyzed SCLEROTIUM rolfsii (SCLEROTIUM rolfsii) gum; chelating agents such as disodium EDTA and the like; the preservative is selected from p-hydroxyacetophenone, E59 and mixtures thereof; solubilizers such as C_12-13Alkanol polyether-9, and the like; fragrances such as geranium (PELARGONIUM GRAVEOLENS) oil and the like; solvents such as water and the like.

Preferably, the mask composition of the present invention comprises the following components in percentage by weight:

wherein E59 consists of 85% propylene glycol, 10% caprylhydroxamic acid, 4% 1, 2-hexanediol, 1% glyceryl caprylate, available from Korea DNE; n-602 consists of 50% dimethiconol, 2% polysorbate-20, 1% 1, 2-hexanediol, 47% water, available from Korea Diamond chemical (KCC).

It will be appreciated by those skilled in the art that the mask composition of the present invention can be prepared by conventional methods in the art. For example, it can be prepared by the following method: 1. weighing water, glycerol, sodium polyacrylate grafted starch, polyacrylate cross-linked polymer-6, PPG-10 methyl glucose ether, trehalose, EDTA disodium, allantoin, sodium hyaluronate, and hydrolyzed SCLEROTIUM rolfsii (SCLEOTIUM ROLFSSII) glue into a main pot, and homogenizing at high speed for 5-8 min; 2. stirring and heating to 88-90 deg.C, sampling, observing the material to obtain a completely transparent uniform liquid, maintaining the temperature, dissolving, sterilizing for 20-30min, and cooling; 3. mixing butanediol and p-hydroxyacetophenone, stirring, preheating to 50 ℃ for dissolving, recovering to room temperature, and stirring uniformly to obtain the finished product 1. When the temperature of the main pot is reduced to 50 ℃, adding the prefabricated product 1 into the main pot, and uniformly stirring; 4. the oil of Pelargonium GRAVEOLENS (Pelargonium GRAVEOLENS) and C12-13-alkanol polyether-9 were mixed and stirred to a completely transparent liquid, which was designated as preform 2. Cooling to 45 deg.C, adding preform 2, E59, N-602, PEG/PPG-17/6 copolymer, glycerol glucoside, herba Centellae (CENTELLA ASIATICA) extract, DS-CERASOME1.0, OPTIMETALTH, and horse chestnut (Aesculus CHINENSIS) extract into main pan, and stirring to obtain facial mask composition.

According to a second aspect of the present invention, there is provided a mask comprising the mask composition of the present invention and a mask carrier. Preferably, the mask of the present invention consists of the mask composition of the present invention and a mask carrier.

Those skilled in the art will appreciate that a variety of mask carriers are available in the art for use in the present invention, such as nonwovens and the like.

It will be appreciated by those skilled in the art that the mask composition of the present invention may be included in a suitable amount, for example, 5 to 50g, preferably 20 to 30 g.

The effective composition of the invention has the following advantages and beneficial effects:

the lactobacillus/soybean milk fermentation product filtrate has the effects of improving the condition of facial microbial flora, reducing facial passing bacteria, reducing main pathogenic bacteria causing skin infection and balancing facial resident bacteria. It contains protein, amino acids and polypeptide, and has good moisture keeping and repairing effects. The ceramide 3 can supplement ceramide in human body, repair skin barrier and improve skin moisture. The centella asiatica extract can act on tissue cells, reduce local inflammatory reaction, reduce local myofibroblast generation, increase healing collagen and the like, and achieves the effects of balancing facial microbial flora, relieving, moisturizing and repairing the skin. The soybean milk lactobacillus fermentation product filtrate, the ceramide 3 and the centella asiatica extract are compounded, and the synergistic effect is achieved in the aspects of relieving and moisturizing.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes or modifications can be made by those skilled in the art after reading the description of the present invention, and such equivalents also fall within the scope of the invention.

Example 1 moisturizing test

Each experimental group mask having the composition shown in the following table was prepared as follows:

1. weighing water, glycerol, sodium polyacrylate grafted starch, polyacrylate cross-linked polymer-6, PPG-10 methyl glucose ether, trehalose, EDTA disodium, allantoin, sodium hyaluronate, and hydrolyzed SCLEROTIUM rolfsii (SCLEOTIUM ROLFSSII) glue into a main pot, and homogenizing at high speed for 5-8 min;

2. stirring and heating to 88-90 deg.C, sampling, observing the material to obtain a completely transparent uniform liquid, maintaining the temperature, dissolving, sterilizing for 20-30min, and cooling;

3. mixing butanediol and p-hydroxyacetophenone, stirring, preheating to 50 ℃ for dissolving, recovering to room temperature, and stirring uniformly to obtain the finished product 1. When the temperature of the main pot is reduced to 50 ℃, adding the prefabricated product 1 into the main pot, and uniformly stirring;

4、mixing Pelargonium GRAVEOLENS (Pelargonium GRAVEOLENS) oil and C_12-13The alkanol polyether-9 was mixed and stirred into a completely clear liquid, which was designated preform 2. Cooling to 45 deg.C, adding preform 2, E59, N-602, PEG/PPG-17/6 copolymer, glycerol glucoside, herba Centellae (CENTELLA ASIATICA) extract, DS-CERASOME1.0, OPTIMETALTH, and horse chestnut (Aesculus CHINENSIS) extract into main pan, and stirring to obtain facial mask composition.

5. Filling the prepared mask composition into common non-woven fabrics according to 25g per piece to obtain the mask.

In addition, each comparative facial mask having the composition shown in the following table was prepared as above.

30 volunteers aged 18-50 years, male and female, no allergy, and no volunteers recently participating in hand test. Before testing, all volunteers clean their arms with clear water, keep the temperature at 20-22 deg.C and humidity at 50-60%, and stand for 30 minutes, and simultaneously select 9 test areas of 4cm by 4cm on the inner sides of the forearms of both hands of the volunteers and mark the test areas.

And respectively transferring 2ml of the facial mask liquid of the prepared comparison groups 1-4 and the prepared facial mask liquid of the prepared experiment groups 1-4 into corresponding areas by using a liquid transfer gun, and uniformly coating the facial mask liquid on the experiment areas by using latex finger sleeves. Test areas not coated with any sample were recorded as blank sets.

And (3) respectively recording the initial value before the mask liquid is not smeared, and the water content of the skin stratum corneum after 30min, 1h, 2h and 3h after smearing by using a Tewameter TM300 water content tester probe.

The average water content of the same area of 30 volunteers was determined and the results are shown in the following table:

note: p is < 0.05 and represents comparison between the product after use and before use

According to the moisturizing test result, the facial mask containing the lactobacillus/soybean milk fermentation product filtrate, the ceramide 3 and the centella asiatica extract has the advantages that the moisturizing effect is obviously improved, and the synergistic moisturizing effect is realized.

Example 2 improvement of facial flora

12 volunteers aged between 20-50 years, male and female were unlimited, any skin, but all were facial skin sensitive skin, with red or pox face, wherein 10 volunteers used the mask of experimental group 1 in example 1 every 2 days, and 2 other volunteers used the mask of comparative group 1 (recorded as volunteer 1 of comparative group 1, volunteer 2 of comparative group 1). After daily cleaning, the non-woven fabric mask is attached to the face, the non-woven fabric mask is torn after being statically applied for 15-20 minutes, and the mask essence is massaged and absorbed for 4 weeks, wherein other products cannot be used in the period.

Samples of the microorganisms were removed from the volunteer face at week 0 and week 4, respectively, and the codes were recorded. Before sampling, the volunteers clean the face with clear water and then rest for 30 minutes in an environment with constant temperature of 22 +/-1 ℃ and constant humidity of 50 +/-3%.

The experimenter wears sterile gloves with both hands, uses sterile cotton swabs to wipe a 4 cm-4 cm area at the flat part of the forehead for a plurality of times, and intercepts facial microorganisms.

The taken microorganism specimen is stored in a microorganism preservation solution, and the percentage distribution of the microorganisms in the sample is analyzed by using microorganism DNA detection, which is shown in the following table:

as can be seen from the above table, when the mask of experimental group 1 in example 1 was used, pseudomonas alcaligenes causing pustule and inflammation on the skin was reduced, and the entire flora was in an equilibrium state.

Example 3 moisturizing and repairing experiments

The volunteers of example 2 above collected TEWL (skin Water loss) (g/m) of the face using a Tewameter TM300 at week 0, week 2 and week 4 after using the mask of experiment group 1 of example 1 and the mask of comparative group 1²H) value.

	Week 0	Week 2	Week 4
				Comparative group 1	17	16	15.5
Experimental group 1	16	14.2*	10.2*

As can be seen from the above table, the skin TEWL value becomes significantly smaller after using the mask of test group 1 in example 1, and the smaller the skin TEWL value, the better the skin barrier, the better the repair, and the better the water-holding capacity.

Example 4 relaxation experiments

54 adult volunteers aged 18-65 years, male and female are not limited, any skin is sensitive skin of face, the skin is randomly divided into 9 groups, 6 volunteers in each group are respectively a control group, a comparison group 1-4 and an experimental group 1-4, all the groups use lactic acid water solution on the nasolabial sulcus part under the condition of no allergy and no damage of the face, and the skin prickling feeling, itching feeling, burning feeling and the like are self-evaluated before and after the facial mask composition of the comparison group 1-4 and the experimental group 1-4 in the embodiment 1 is used for one time on the face (the control group), so that the relieving effect of the facial mask composition on the sensitive skin is evaluated. The specific operation is as follows:

the face of the volunteer is cleaned by clear water and then is rested for 30 minutes in the environment with the constant temperature of 22 +/-1 ℃ and the constant humidity of 50 +/-3%.

A cosmetic cotton pad stained with 2ml of 10% lactic acid was placed on the lateral nasolabial folds, and rubbed gently for 7 times to set a timer.

The volunteers scored discomfort such as skin prickling, burning, itching, etc. 2 minutes, 5 minutes, and 8 minutes after application, respectively. Grading standard: no stinging sensation was scored at 0, mild stinging at 1, moderate stinging at 2, and severe stinging at 3.

Immediately after the discomfort (score of nasolabial fold was more than 2 points), 3ml of water or the mask composition of comparative group 1-4 and experimental group 1-4 of example 1 was transferred to the abdomen of the finger using a syringe, smeared on the nasolabial fold, smeared once gently, and a second timer was started.

Each group of volunteers scored again at 2 minutes, 5 minutes, 8 minutes after application of the water or mask composition according to the scoring criteria above and recorded.

The average of the scores for each group of volunteers at 2 min, 5 min, 8min was as follows:

	2min	5min	8min
				control group	2	2	2
Comparative group 1	2	1.6	1.3
				Comparative group 2	1.8	1.2	0
Comparative group 3	1.7	1.2	0
				Comparative group 4	1.6	1	0
Experimental group 1	1.5	0	0
				Experimental group 2	1.4	0	0
Experimental group 3	1.4	0	0
				Experimental group 4	1.5	0	0

According to the relieving experiment result, the mask composition disclosed by the invention can be used for effectively relieving the tingling sensation and has a good relieving effect.

The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiment. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A facial mask composition characterized by containing a filtrate of lactic acid bacteria fermentation product of soybean milk, ceramide 3 and centella asiatica extract.

The lactobacillus fermented product filtrate of soybean milk is preferably black soybean milk lactobacillus fermented product filtrate, and further preferably optimelath (oxitenmin).

The ceramide 3 is preferably liposome-encapsulated ceramide 3, and more preferably DS-CERASOME 1.0.

2. The mask composition of claim 1, wherein the weight percentage of the filtrate of the lactobacillus ferments of soy milk is 0.5-5.0%, preferably 1.0-2.0%; the weight percentage content of the ceramide 3 is 0.2-2.0%, preferably 0.5-1.0%; the content of centella asiatica extract is 0.2-2.0 wt%, preferably 0.5-1.0 wt%.

3. The mask composition of claim 1 or 2, further comprising a humectant.

Preferably, the humectant is present in an amount of 5 to 22% by weight.

Preferably, the humectant is one or a mixture of two or more of glycerol, trehalose, allantoin, sodium hyaluronate and butanediol.

4. The mask composition of claim 1 or 2, further comprising an emollient.

Preferably, the emollient is present in an amount of 3.0 to 17.0% by weight.

Preferably, the emollient is selected from one or a mixture of two or more of PPG-10 methyl glucose ether, N-602, PEG/PPG-17/6 copolymer, and glycerol glucoside.

5. The mask composition of claim 1 or 2, further comprising a soothing anti-inflammatory agent.

Preferably, the soothing anti-inflammatory agent is present in an amount of 0.01 to 1.0% by weight.

Preferably, the soothing anti-inflammatory agent is horse chestnut (AESCULUS CHINENSIS) extract.

6. The mask composition of claim 1 or 2, further comprising a moisturizing agent, an emollient, and a soothing anti-inflammatory agent.

Preferably, the humectant is 5-22 wt%, the emollient is 3.0-17.0 wt%, and the soothing anti-inflammatory agent is 0.01-1.0 wt%.

Preferably, the humectant is one or a mixture of two or more of glycerol, trehalose, allantoin, sodium hyaluronate and butanediol; the emollient is selected from one or two or more of PPG-10 methyl glucose ether, N-602, PEG/PPG-17/6 copolymer, and glycerol glucoside; the soothing anti-inflammatory agent is horse chestnut (Aesculus CHINENSIS) extract.

7. The mask composition of any one of claims 3-6, further comprising a thickening agent, a chelating agent, a preservative, a solubilizer, a fragrance, and a solvent.

Preferably, the thickener is present in an amount of 0.1 to 1.0% by weight, the chelating agent is present in an amount of 0.01 to 1.0% by weight, the preservative is present in an amount of 0.5 to 1.5% by weight, the solubilizer is present in an amount of 0.001 to 0.1% by weight, and the fragrance is present in an amount of 0.0001 to 0.001% by weight.

Preferably, the thickener is selected from one or a mixture of two or more of sodium polyacrylate grafted starch, polyacrylate cross-linked polymer-6, and hydrolyzed SCLEROTIUM rolfsii (SCLEROTIUM rolfsii) gum; chelating agents such as disodium EDTA and the like; the preservative is selected from p-hydroxyacetophenone, E59 and mixtures thereof; the solubilizer is C_12-13Alkanol polyether-9, and the like; the aromatic agent is Pelargonium GRAVEOLENS (Pelargonium GRAVEOLENS) oil; the solvent is water.

8. The mask composition of claim 7, comprising the following components in weight percent:

9. a mask comprising the mask composition of any one of claims 1-8 and a mask carrier.

10. The mask pack according to claim 9, wherein the mask pack contains 5 to 50g, preferably 20 to 30g of the mask composition.