CN110448522A - A kind of jelly peptide restructuring compound for the neck and preparation method thereof - Google Patents
A kind of jelly peptide restructuring compound for the neck and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The present invention provides a kind of jelly peptide restructuring compounds for the neck and preparation method thereof, it is related to biological cell technical field of skin care, freeze peptide solution and mixed plant extract solution including mescenchymal stem cell, extract freeze-drying powder makes the activity of stem cell extract and function be saved and be utilized, effectively extend its function and effect, and by with the niacinamide that accordingly matches, purslane extract, Gotu Kola P.E, panthenol, allantoin, tocopherol, Sodium Hyaluronate, hydrolytic collagen, Bacillus acidi lactici/soya-bean milk tunning filtrate and bacillus/fermented soybean product extract carry out being mixed into paste in proper temperature environment, neck neck line is effectively improved so that freezing peptide restructuring compound for the neck and reaching, the effect of even skin tone degree and smoothness.
Description
Technical field
The present invention relates to biological cell technical field of skin care more particularly to a kind of jelly peptide restructuring compound for the neck and preparation methods.
Background technique
Stem cell is most important for the skin of women, and skin keeps young, the complete and later reparation that goes wrong
Stem cell will be leaned on, and many obstinate general skin care item of skin problem can't resolve, if fundamentally solving phase
The skin problem answered depends on reparation skin, and the skin repair ability of mescenchymal stem cell is just very strong, and exists in recent years
The research of skin injury field is also more and more extensive, and business application effect is also very significant.
Mescenchymal stem cell belongs to multipotential stem cell, has Multidirectional Differentiation, hematopoiesis support and promotes stem cell implantation, is immunized
The multiple functions such as regulation, are primarily present in placenta, umbilical cord, fat and marrow；Mescenchymal stem cell can divide during the growth process
Several hundred kinds of cell factors are secreted, these cell factors can promote the growth of skin histology differentiation, angiogenesis and granulation tissue,
To promote the structural remodeling and Regeneration and Repair of injured cutaneous tissue, the generation of scar tissue is reduced.For various acute and chronic skins
Skin damage, the reparation of eczema, allergy and ulcer.And the immune function of local organization can be adjusted, reduce the thin of wound tissue
Bacterium infection and inflammatory reaction.
Extract includes the vesicles that the diameter of cell secretion is 30~150nm, includes albumen relevant from cell
The biomolecule such as matter and nucleotide, play an important role between cell communication.Extract is the atom membrane vesicle of cell secretion, with
Mescenchymal stem cell is compared, and extract volume is smaller, complexity is lower, is readily produced and is stored, mescenchymal stem cell extract
Rich in bioactive molecule, such as protein and RNA, many protein being present in extract belong to enzyme, have catalysis
Activity.But a generally existing problem is that the bioactivity of cell factor and extract is difficult to continuous action at present, is caused
Its function and effect endurance is lower；Now using computer or phone as crowd's substantial amounts of workbench in city,
And working condition usually maintains one to bow or micro- movement bowed, therefore the neck neck of the office worker of existing youth is general
There are deep mixed neck lines, and there is also the unsmooth and unequal skin problems of the colour of skin for skin of neck, for the female to like to be beautiful
Property for, the skin problem of neck, which becomes, influences to show beautiful key factor, thus how to extend cell factor and
It extracts the preservation of microbic activity and its restructuring compound for the neck for forming reparation neck skin with other effective components is become into this field skill
Art personnel's urgent problem to be solved.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art, provide a kind of jelly peptide restructuring compound for the neck and
Preparation method, the restructuring compound for the neck realize the effect of being effectively improved neck neck line, even skin tone degree and smoothness.
The present invention is to be achieved by the following technical programs: a kind of jelly peptide restructuring compound for the neck, by volume parts ratio, including 7-16 parts
Mescenchymal stem cell freezes peptide solution and 84-93 parts of mixed plant extract solutions, and by mass fraction ratio, mescenchymal stem cell freezes
Peptide solution includes 10-50 parts of extract freeze-drying powders and 50-90 parts of distilled water, and by mass parts ratio, mixed plant extract is molten
Liquid includes 0.5-2.5 parts of niacinamide, 0.5-2 parts of purslane extracts, 0.5-2 parts of Gotu Kola P.Es, 0.5-2.5 parts of panthenols,
0.3-1.5 parts of allantoins, 0.5-3 parts of tocopherols, 0.5-2 parts of Sodium Hyaluronates, 0.5-2 parts of hydrolytic collagens, 0.5-2.5 parts of lactic acid
Bacillus/soya-bean milk tunning filtrate, 0.5-2.5 parts of bacillus/fermented soybean product extract and 77.5-95.2 parts of distillations
Water, extract freeze-drying powder are made by the culture processing of mescenchymal stem cell starvation, and mescenchymal stem cell is to extract from Goat Placenta
Mescenchymal stem cell is cultivated to obtain according to the method normally cultivated.
According to the above technical scheme, it is preferable that press volume parts ratio, including 8-13 part mescenchymal stem cells jelly peptide solutions with
And 87-92 parts of mixed plant extract solutions, by mass fraction ratio, it includes 18-40 parts of extractions that mescenchymal stem cell, which freezes peptide solution,
Object freeze-dried powder and 60-82 parts of distilled water, by mass parts ratio, mixed plant extract solution includes 0.8-2.2 parts of niacinamide,
0.8-1.6 parts of purslane extracts, 0.7-1.5 parts of Gotu Kola P.Es, 0.6-2.2 parts of panthenols, 0.4-1.2 parts of allantoins,
0.7-2 parts of tocopherols, 0.7-1.5 parts of Sodium Hyaluronates, 0.8-1.5 parts of hydrolytic collagens, 0.8-2.2 parts of Bacillus acidi lacticis/soya-bean milk hair
Ferment Product filtrate, 0.7-2.3 parts of bacillus/fermented soybean product extracts and 81.8-93 parts of distilled water, extract freeze-drying
Powder is made by the culture processing of mescenchymal stem cell starvation, and mescenchymal stem cell is the mescenchymal stem cell for extracting from Goat Placenta,
It is cultivated to obtain according to the method normally cultivated.
According to the above technical scheme, it is preferable that the time of mescenchymal stem cell starvation culture is 12-24 hours.
According to the above technical scheme, it is preferable that the time that mescenchymal stem cell is normally cultivated is 48-72 hours.
According to the above technical scheme, it is preferable that the stem cell factor ingredient for including in extract freeze-drying powder is freeze-drying shape
State.
According to the above technical scheme, it is preferable that the jelly peptide restructuring compound for the neck is mainly used for improving neck line, the even skin tone of skin of neck
And smoothness.
A kind of preparation method for freezing peptide restructuring compound for the neck, comprising the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and body
Product specific concentration is that 8%-12% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask in 35-38 DEG C, 3-6%CO2 item
It is cultivated under part, incubation time is 48-72 hours；
The preparation of extract freeze-drying powder: reaching 70%-80% to growth of mesenchymal stem cells degree, clear using physiological saline
It washes mescenchymal stem cell 2-3 times, hungry culture 12-24 hours is carried out to mescenchymal stem cell with Multiple electrolytes injection, to
After cell extent of growth reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, is collected in cell culture after the completion of centrifugation
Clear liquid obtains stem cell factor, and is extracted using extract extracts kit to mescenchymal stem cell extract, among it
Mesenchymal stem cells extract is mainly excretion body, and the stem cell factor of extraction and excretion body are then put into freeze-drying after mixing
It is lyophilized in machine, forms extract freeze-drying powder；
Mescenchymal stem cell jelly peptide solution preparation: press mass fraction ratio, measure 10-50 parts of extract freeze-drying powders and
50-90 parts of distilled water, 16-25 DEG C at a temperature of stir evenly obtained mescenchymal stem cell and freeze peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures 0.5-2.5 parts of niacinamide, 0.5-2 parts of dents
Amaranth extract, 0.5-2 parts of Gotu Kola P.Es, 0.5-2.5 parts of panthenols, 0.3-1.5 parts of allantoins, 0.5-3 parts of tocopherols, 0.5-
2 parts of Sodium Hyaluronates, 0.5-2 parts of hydrolytic collagens, 0.5-2.5 parts of Bacillus acidi lacticis/soya-bean milk tunning filtrate, 0.5-2.5 parts of buds
Spore bacillus/fermented soybean product extract and 77.5-95.2 parts of distilled water, it is molten to stir evenly obtained mixed plant extract
Liquid；
Freeze the preparation of peptide restructuring compound for the neck: pressing volume parts ratio, successively measures 7-16 parts of mescenchymal stem cells and freeze peptide solution and 84-93
Part mixed plant extract solution is put into mixing arrangement, and obtained paste is stirred evenly under room temperature.
The beneficial effects of the present invention are: all cytokine antagonist polypeptides of mescenchymal stem cell secretion and extract etc. are had
Effect ingredient whole high efficiency extraction is simultaneously lyophilized, and extract freeze-drying powder protects the activity of stem cell extract and function
Deposit and utilize, and the time survived can be longer, is able to extend its function and effect, and by with the nicotinoyl that accordingly matches
Amine, purslane extract, Gotu Kola P.E, panthenol, allantoin, tocopherol, Sodium Hyaluronate, hydrolytic collagen, Bacillus acidi lactici/
Soya-bean milk tunning filtrate and bacillus/fermented soybean product extract carry out being mixed into paste in normal temperature environment, from
And to freeze the curative effect that peptide restructuring compound for the neck is effectively improved neck neck line, even skin tone degree and smoothness.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, below with reference to embodiment pair
The present invention is described in further detail.
Embodiment 1:
The present invention provides a kind of jelly peptide restructuring compounds for the neck, freeze peptide solution, 93 by volume parts ratio, including 7 parts of mescenchymal stem cells
Part mixed plant extract solution, by mass fraction ratio, it includes 10 parts of extracts freeze-dryings that the mescenchymal stem cell, which freezes peptide solution,
Powder and 90 parts of distilled water, by mass parts ratio, the mixed plant extract solution includes 0.5 part of niacinamide, 0.5 part of dent
Amaranth extract, 0.5 part of Gotu Kola P.E, 0.5 part of panthenol, 0.3 part of allantoin, 0.5 part of tocopherol, 0.5 part of Sodium Hyaluronate,
0.5 part of hydrolytic collagen, 0.5 part of Bacillus acidi lactici/soya-bean milk tunning filtrate, 0.5 part of bacillus/fermented soybean product extract
And 95.2 parts of distilled water, the extract freeze-drying powder are made by the culture processing of mescenchymal stem cell starvation, starvation culture
Time is 12 hours, and the mescenchymal stem cell is the mescenchymal stem cell for extracting from Goat Placenta, according to the method normally cultivated
It is cultivated to obtain, incubation time is 48 hours.
Embodiment 2:
The present invention provides a kind of jelly peptide restructuring compounds for the neck, freeze peptide solution, 84 by volume parts ratio, including 16 parts of mescenchymal stem cells
Part mixed plant extract solution, presses mass fraction ratio, mescenchymal stem cell freeze peptide solution including 50 parts of extract freeze-drying powders with
And 50 parts of distilled water, by mass parts ratio, mixed plant extract solution includes 2.5 parts of niacinamide, 2 parts of purslane extracts, and 2
Part Gotu Kola P.E, 2.5 parts of panthenols, 1.5 parts of allantoins, 3 parts of tocopherols, 2 parts of Sodium Hyaluronates, 2 parts of hydrolytic collagens, 2.5
Part Bacillus acidi lactici/soya-bean milk tunning filtrate, 2.5 parts of bacillus/fermented soybean product extract and 77.5 parts of distilled water,
Extract freeze-drying powder is made by the culture processing of mescenchymal stem cell starvation, and the time of starvation culture is 16 hours, and mesenchyma is dry
Cell is the mescenchymal stem cell for extracting from Goat Placenta, is cultivated to obtain according to the method normally cultivated, incubation time 56
Hour.
Embodiment 3:
The present invention provides a kind of jelly peptide restructuring compounds for the neck, freeze peptide solution, 91 by volume parts ratio, including 12 parts of mescenchymal stem cells
Part mixed plant extract solution, presses mass fraction ratio, mescenchymal stem cell freeze peptide solution including 35 parts of extract freeze-drying powders with
And 65 parts of distilled water, by mass parts ratio, mixed plant extract solution includes 1 part of niacinamide, 1 part of purslane extract, and 1 part
Gotu Kola P.E, 1.5 parts of panthenols, 0.5 part of allantoin, 1 part of tocopherol, 1 part of Sodium Hyaluronate, 1 part of hydrolytic collagen, 1.5 parts
Bacillus acidi lactici/soya-bean milk tunning filtrate, 1.5 parts of bacillus/fermented soybean product extract and 89 parts of distilled water extract
Object freeze-dried powder is made by the culture processing of mescenchymal stem cell starvation, and the time of starvation culture is 24 hours, mescenchymal stem cell
It for the mescenchymal stem cell for extracting from Goat Placenta, is cultivated to obtain according to the method normally cultivated, incubation time is 72 hours.
According to above-described embodiment, it is preferable that the stem cell factor ingredient for including in extract freeze-drying powder is lyophilised state,
And extract freeze-drying powder is placed in -20 DEG C of preservations, under the temperature environment, can effectively extend in extract freeze-drying powder stem cell because
The activity of son and holding time.
Further, mainly for the preparation of the restructuring compound for the neck of skin of neck injury repair, this kind of effect can be tied by test
Fruit carries out clear conclusion.
A kind of preparation method for freezing peptide restructuring compound for the neck, comprising the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and body
Product specific concentration is that 10% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask and is trained under the conditions of 37 DEG C, 5%CO2
It supports, incubation time is 48-72 hours；
The preparation of extract freeze-drying powder: reaching 70%-80% to growth of mesenchymal stem cells degree, clear using physiological saline
It washes mescenchymal stem cell 2-3 times, hungry culture 12-24 hours is carried out to mescenchymal stem cell with Multiple electrolytes injection, to
After cell extent of growth reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, is collected in cell culture after the completion of centrifugation
Clear liquid obtains stem cell factor, and is extracted using extract extracts kit to mescenchymal stem cell extract, among it
Mesenchymal stem cells extract is mainly excretion body, and the stem cell factor of extraction and excretion body are then put into freeze-drying after mixing
It is lyophilized in machine, forms extract freeze-drying powder；
Mescenchymal stem cell jelly peptide solution preparation: press mass fraction ratio, measure 10-50 parts of extract freeze-drying powders and
50-90 parts of distilled water, 16-25 DEG C at a temperature of stir evenly obtained mescenchymal stem cell and freeze peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures niacinamide, purslane extract, centella
Extract, panthenol, allantoin, tocopherol, Sodium Hyaluronate, hydrolytic collagen, Bacillus acidi lactici/soya-bean milk tunning filtrate, gemma
Bacillus/fermented soybean product extract and distilled water stir evenly obtained mixed plant extract solution；
Freeze the preparation of peptide restructuring compound for the neck: pressing volume parts ratio, successively measures mescenchymal stem cell jelly peptide solution and mixed plant mentions
It takes object solution to be put into mixing arrangement, obtained paste is stirred evenly under room temperature.
Sensitivity test
The first step test first 100 small white mouse back side shavings and smear embodiment 1-3 proportion generate jelly peptide restructuring compound for the neck,
Testing staff observes the small white mouse back side and does not generate the adverse reactions such as red and swollen, acne；Second test, chooses 60 20-45 weeks
The adult human in year tests, and 60 testing crews are divided into 3 groups of every group of 20 people, carry out the production of above-mentioned 3 groups of embodiments respectively
Product anaphylaxis detects, and the jelly peptide restructuring compound for the neck of corresponding embodiment is smeared on the neck of all testers, and testing staff detects survey at any time
The neck change of skin of examination personnel, after tested personnel and testing staff identify the neck of all testers without occur it is red and swollen or
Person plays the adverse reactions such as acne, therefore the jelly peptide restructuring compound for the neck will not generate adverse reaction to human body.
Curative effect test
In view of women for the distinguishing ability of cosmetics, thus choose 27-35 years old, 36-40 years old and 41-45 years old each 60
Women tests, and the women of three groups of age brackets is separated into 3 groups of 20 people's test cells for using embodiment 1-3 respectively, in use,
The restructuring compound for the neck of corresponding embodiment is smeared at the neck skin of tester, once a day, is gently rubbed 20 minutes every time, is persistently used one
Month, it is then scored respectively wrinkle degree of improvement, even skin tone degree and smoothness three using women, repairs skin degree
For three average marks, the total score of each single item is 100 points, makes scoring according to using effect, and 0-30 divides that effect is unobvious, 31-
60 points of effects are common, 61-80 points of effect is obvious, 81-100 divides highly significant and the more high effect that scores is better, calculate each survey
The average mark for trying unit, obtains the test result such as table 1.
By to table 1 data analysis, the jelly peptide restructuring compound for the neck for wrinkle degree of improvement, even skin tone degree and smoothness this
Class skin characteristic has effects that obvious, and for wrinkle degree of improvement index, the smoothness test effect of all age group is close, still
The moistening effect of embodiment 3 is substantially better than other two groups of embodiments；For even skin tone degree and smoothness, in a certain range
The interior increase with the age, the effect of the restructuring compound for the neck is more obvious, and the effect of embodiment 3 is substantially better than embodiment 1 and embodiment
2, show that embodiment 3 is most preferred embodiment in conjunction with table 1.
Specific tester 1:
Name: Zheng little Jie age: 27
Skin quality: staying up late, it is relatively deep to like that evening sleeps love neck line of bowing
Service life: all using sooner or later, persistently uses one month, and cooperates massage 20 minutes
Effect: after one month through with photo comparison before, neck line is obviously thin out, and the colour of skin is gradually uniform
Specific tester 2:
Name: Qian little Jie age: 32
Skin quality: the dry line of neck microgroove
Service life: it is sooner or later each to smear 1 time, and cooperate massage 20 minutes
Effect: restructuring compound for the neck quality is soft and smooth, by with photo comparison before, the reduction of small microgroove, deeper lines after adhering to 1 month
Also it can shoal
Specific tester 3:
Name: Zhou Nvshi age: 40
Skin quality: neck relaxation neck line is deeper
Service life: smearing once before sleeping every night, and is used continuously 4 weeks
Effect: restructuring compound for the neck has greasy feeling when being just applied to skin of neck, and with massage, the restructuring compound for the neck of smearing is absorbed quickly, presses
After rubbing, neck skin becomes very soft, by with photo comparison before, promote effect after four weeks and neck line fading effect be bright
It is aobvious, it is also very effective for pigmentation.
The beneficial effects of the present invention are: all cytokine antagonist polypeptides of mescenchymal stem cell secretion and extract etc. are had
Effect ingredient whole high efficiency extraction is simultaneously lyophilized, and extract freeze-drying powder protects the activity of stem cell extract and function
Deposit and utilize, and the time survived can be longer, is able to extend its function and effect, and by with the nicotinoyl that accordingly matches
Amine, purslane extract, Gotu Kola P.E, panthenol, allantoin, tocopherol, Sodium Hyaluronate, hydrolytic collagen, Bacillus acidi lactici/
Soya-bean milk tunning filtrate and bacillus/fermented soybean product extract carry out being mixed into paste in normal temperature environment, from
And to freeze the curative effect that peptide restructuring compound for the neck is effectively improved neck neck line, even skin tone degree and smoothness.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of jelly peptide restructuring compound for the neck, which is characterized in that press volume parts ratio, including 7-16 part mescenchymal stem cells jelly peptide solutions and
84-93 parts of mixed plant extract solutions, by mass fraction ratio, the mescenchymal stem cell jelly peptide solution includes 10-50 parts and mentions
Object freeze-dried powder and 50-90 parts of distilled water are taken, by mass parts ratio, the mixed plant extract solution includes 0.5-2.5 parts
Niacinamide, 0.5-2 parts of purslane extracts, 0.5-2 parts of Gotu Kola P.Es, 0.5-2.5 parts of panthenols, 0.3-1.5 parts of allantoins,
0.5-3 parts of tocopherols, 0.5-2 parts of Sodium Hyaluronates, 0.5-2 parts of hydrolytic collagens, 0.5-2.5 parts of Bacillus acidi lacticis/soya-bean milk fermentation produce
Object filtrate, 0.5-2.5 parts of bacillus/fermented soybean product extract and 77.5-95.2 parts of distilled water, the extract freeze
Dry powder is made by the culture processing of mescenchymal stem cell starvation, the mescenchymal stem cell be extract from Goat Placenta mesenchyma it is dry
Cell is cultivated to obtain according to the method normally cultivated.
2. a kind of jelly peptide restructuring compound for the neck according to claim 1, which is characterized in that press volume parts ratio, including 8-13 parts of mesenchymas
Stem cell freezes peptide solution and 87-92 parts of mixed plant extract solutions, and by mass fraction ratio, the mescenchymal stem cell freezes peptide
Solution includes 18-40 parts of extract freeze-drying powders and 60-82 parts of distilled water, by mass parts ratio, the mixed plant extract
Solution includes 0.8-2.2 parts of niacinamide, 0.8-1.6 parts of purslane extracts, 0.7-1.5 parts of Gotu Kola P.Es, and 0.6-2.2 parts
Panthenol, 0.4-1.2 parts of allantoins, 0.7-2 parts of tocopherols, 0.7-1.5 parts of Sodium Hyaluronates, 0.8-1.5 parts of hydrolytic collagens, 0.8-
2.2 parts of Bacillus acidi lacticis/soya-bean milk tunning filtrate, 0.7-2.3 parts of bacillus/fermented soybean product extract and 81.8-
93 parts of distilled water, the extract freeze-drying powder are made by the culture processing of mescenchymal stem cell starvation, the mescenchymal stem cell
For the mescenchymal stem cell for extracting from Goat Placenta, cultivated to obtain according to the method normally cultivated.
3. a kind of jelly peptide restructuring compound for the neck according to claim 2, which is characterized in that mescenchymal stem cell starvation culture when
Between be 12-24 hours.
4. a kind of jelly peptide restructuring compound for the neck according to claim 3, which is characterized in that the mescenchymal stem cell normally cultivate when
Between be 48-72 hours.
5. a kind of jelly peptide restructuring compound for the neck according to claim 4, which is characterized in that include in the extract freeze-drying powder is dry
Cytokine component is lyophilised state.
6. a kind of jelly peptide restructuring compound for the neck according to claim 5, which is characterized in that the jelly peptide restructuring compound for the neck is mainly used for improving neck skin
Neck line, even skin tone and the smoothness of skin.
7. a kind of preparation method for freezing peptide restructuring compound for the neck, which comprises the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and volume ratio
Concentration is that 8%-12% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask under the conditions of 35-38 DEG C, 3-6%CO2
It is cultivated, incubation time is 48-72 hours；
The preparation of extract freeze-drying powder: reach 70%-80% to growth of mesenchymal stem cells degree, between cleaning using physiological saline
Mesenchymal stem cells 2-3 times carry out hungry culture 12-24 hours to mescenchymal stem cell with Multiple electrolytes injection, to cell
After extent of growth reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, cell culture supernatant is collected after the completion of centrifugation
Stem cell factor is obtained, and mescenchymal stem cell extract is extracted using extract extracts kit, wherein mesenchyma
Stem cell extract is mainly excretion body, is then put into the stem cell factor of extraction and excretion body in freeze dryer after mixing
It is lyophilized, forms extract freeze-drying powder；
The preparation of mescenchymal stem cell jelly peptide solution: mass fraction ratio is pressed, 10-50 parts of extract freeze-drying powders and 50-90 are measured
Part distilled water, 16-25 DEG C at a temperature of stir evenly obtained mescenchymal stem cell and freeze peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures 0.5-2.5 parts of niacinamide, and 0.5-2 portions of purslanes mention
Take object, 0.5-2 parts of Gotu Kola P.Es, 0.5-2.5 parts of panthenols, 0.3-1.5 parts of allantoins, 0.5-3 parts of tocopherols, 0.5-2 parts
Sodium Hyaluronate, 0.5-2 part hydrolytic collagen, 0.5-2.5 parts of Bacillus acidi lacticis/soya-bean milk tunning filtrate, 0.5-2.5 parts of gemma bars
Bacterium/fermented soybean product extract and 77.5-95.2 parts of distilled water, stir evenly obtained mixed plant extract solution；
Freeze the preparation of peptide restructuring compound for the neck: pressing volume parts ratio, successively measures 7-16 parts of mescenchymal stem cells jelly peptide solutions and 84-93 parts mixed
It closes plant extract solution to be put into mixing arrangement, obtained paste is stirred evenly under room temperature.
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