CN110403898A - A kind of jelly peptide facial mask and preparation method thereof - Google Patents
A kind of jelly peptide facial mask and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
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- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A61Q19/007—Preparations for dry skin
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- A—HUMAN NECESSITIES
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The present invention provides a kind of jelly peptide facial masks and preparation method thereof, it is related to biological cell technical field of skin care, freeze peptide solution including mescenchymal stem cell, mixed plant extract solution, glycerite and facial mask substrate, it includes extract freeze-drying powder that mescenchymal stem cell, which freezes peptide solution, extract freeze-drying powder makes the activity of stem cell extract and function be saved and be utilized, effectively extend its function and effect, and by with the cigarette Gotu Kola P.E that accordingly matches, german chamomile flower extract, polygonum cuspidate root extract and a kind of reed mentioned in ancient books root extract mix and adsorbed by facial mask substrate in proper temperature environment, then facial mask entirety frozen dried, so that freezing peptide facial mask reaches effective moisturizing, it lightens the stain, wrinkle etc. is gone to repair the curative effect of skin problem.
Description
Technical field
The present invention relates to biological cell technical field of skin care more particularly to a kind of jelly peptide facial mask and preparation method thereof.
Background technique
Facial mask is a kind of carrier of face beautifying health keeping product, powder reconciliation at present, kaolin, non-woven fabrics, silk mask, tencel face
Film, Biofibre facial mask, nonwoven fabric facial mask material facial mask using very extensive.Superconducting film facial mask is the skill of latest generation innovation
Art.
Facial mask is a classification in skin care item.Its most basic purpose is to make up makeup removing and wash one's face still insufficient clear
Clean work cooperates other essential ingredients to realize other maintenance functions on this basis, for example, replenishing water and preserving moisture, whitening and anti-aging,
Sage Extract etc..
Result of study shows that stem cell is most important for the skin of women at present, and skin keeps young, complete and goes out
The later reparation of existing problem will lean on stem cell, and many obstinate general skin care item of skin problem can't resolve, and
It tackles the problem at its root or to repair skin, the skin repair ability of mescenchymal stem cell is just very strong, and exists in recent years
Skin injury field using more and more extensive, effect is also very significant.
Wherein mescenchymal stem cell belongs to multipotential stem cell, have Multidirectional Differentiation, hematopoiesis support and promote stem cell implantation,
The multiple functions such as immunoregulation are primarily present in placenta, umbilical cord, fat and marrow；Mescenchymal stem cell is during the growth process
Several hundred kinds of cell factors can be secreted, these cell factors can promote skin histology differentiation, angiogenesis and granulation tissue
Growth, to promote the structural remodeling and Regeneration and Repair of injured cutaneous tissue, reduces the generation of scar tissue.It is slow for various urgency
Property skin injury, the reparation of eczema, allergy and ulcer.And the immune function of local organization can be adjusted, reduce wound tissue
Bacterium infection and inflammatory reaction.
Mescenchymal stem cell extract includes the vesicles that the diameter of cell secretion is 30~150nm, is included from thin
The biomolecule such as the relevant protein of born of the same parents and nucleotide, play an important role between cell communication.Extract is the small of cell secretion
Biological membrane vesicle, compared with mescenchymal stem cell, extract volume is smaller, complexity is lower, is readily produced and stores, mesenchyma
Stem cell extract is rich in bioactive molecule, and such as protein and RNA, many protein being present in extract belong to enzyme
Class has catalytic activity.But a generally existing problem is that the bioactivity of cell factor and extract is difficult to hold at present
Continuous effect, causes its function and effect endurance lower, although facial mask has been widely used in cosmetics, in view of stem cell
The characteristic of extract is still difficult to that effectively device is applied on facial mask, therefore how to extend cell factor and extract life
It is simultaneously used cooperatively by the active preservation of object with other compositions, and the facial mask comprising stem cell extract is made to reach the work of skin repair
With becoming those skilled in the art's urgent problem to be solved.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art, provide a kind of jelly peptide facial mask and
Preparation method, the jelly peptide facial mask are capable of the effective active of long-term preservation stem cell extract, and by matching with other compositions
Than, using simple, effectively realization moisturizing, the curative effect for repairing skin such as lighten the stain, go wrinkle.
The present invention is to be achieved by the following technical programs: a kind of jelly peptide facial mask, by volume parts ratio, including 5-10 parts
Mescenchymal stem cell freezes peptide solution, 86-94 parts of mixed plant extract solutions, 1-4 parts of glycerites and facial mask substrate, by matter
Score ratio is measured, it includes 10-25 parts of extract freeze-drying powders and 75-90 parts of distilled water that the mescenchymal stem cell, which freezes peptide solution, is pressed
Mass parts ratio, the mixed plant extract solution include 1-3 parts of Gotu Kola P.Es, 0.5-2 parts of german chamomile flower extracts,
0.5-2 parts of polygonum cuspidate root extracts, 0.5-2 parts of a kind of reed mentioned in ancient books root extracts and 91-97.5 parts of distilled water, by mass parts ratio, glycerol is molten
Liquid includes 40-60 parts of glycerol and 40-60 parts of distilled water, and the extract freeze-drying powder is handled by the culture of mescenchymal stem cell starvation
It is made, the mescenchymal stem cell extracts from the mescenchymal stem cell of Goat Placenta, and is cultivated according to the method normally cultivated
It obtains.
According to the above technical scheme, it is preferable that press volume parts ratio, including 6-8 part mescenchymal stem cells jelly peptide solutions,
88-91 parts of mixed plant extract solutions, 1-3 parts of glycerites and facial mask substrate, by mass fraction ratio, the mesenchyma is dry
It includes 15-22 parts of extract freeze-drying powders and 78-85 parts of distilled water that cell, which freezes peptide solution, and by mass parts ratio, the mixing is planted
Object extract solution includes that 1.5-2.5 parts of Gotu Kola P.Es, 0.8-1.8 parts of german chamomile flower extracts, 0.7-1.8 parts of polygoni cuspidati,radixes mention
Object, 0.7-1.8 parts of a kind of reed mentioned in ancient books root extracts and 92.1-96.3 parts of distilled water are taken, by mass parts ratio, glycerite includes 42-58
Part glycerol and 42-58 parts of distilled water, the extract freeze-drying powder is made by the culture processing of mescenchymal stem cell starvation, between described
Mesenchymal stem cells extract from the mescenchymal stem cell of Goat Placenta, and are cultivated to obtain according to the method normally cultivated.
According to the above technical scheme, it is preferable that the time of the mescenchymal stem cell starvation culture is 12-24 hours.
According to the above technical scheme, it is preferable that the incubation time of the mescenchymal stem cell is 48-72 hours.
According to the above technical scheme, it is preferable that the jelly peptide facial mask facial mask integrally passes through frozen dried, and extract is lyophilized
Powder is placed in -20 DEG C of preservations.
A kind of preparation method for freezing peptide facial mask, comprising the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and body
Product specific concentration is that 8%-12% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask in 35-38 DEG C, 3-6%CO2 item
It is cultivated under part, incubation time is 48-72 hours；
The preparation of extract freeze-drying powder: reaching 70%-80% to growth of mesenchymal stem cells degree, clear using physiological saline
It washes mescenchymal stem cell 2-3 times, hungry culture 12-24 hours is carried out to mescenchymal stem cell with Multiple electrolytes injection, to
After cell extent of growth reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, is collected in cell culture after the completion of centrifugation
Clear liquid obtains stem cell factor, and is extracted using extract extracts kit to mescenchymal stem cell extract, among it
Mesenchymal stem cells extract is mainly excretion body, and the stem cell factor of extraction and excretion body are then put into freeze-drying after mixing
It is lyophilized in machine, forms extract freeze-drying powder, which can long-term preservation；
Mescenchymal stem cell jelly peptide solution preparation: press mass fraction ratio, weigh 10-25 parts of extract freeze-drying powders and
75-90 parts of distilled water are stirred into mescenchymal stem cell at normal temperature and freeze peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures 1-3 parts of Gotu Kola P.Es, 0.5-2 parts of mothers
Chrysanthemum extract, 0.5-2 parts of polygonum cuspidate root extracts, 0.5-2 parts of a kind of reed mentioned in ancient books root extracts and the 91-97.5 parts of distilled water systems of stirring evenly
；
The preparation of glycerite: pressing mass fraction ratio, weigh 40-60 part glycerol and 40-60 parts of distilled water, general when stirring
Glycerol is dissolved in distilled water, is stirred evenly obtained；
Freeze the preparation of peptide facial mask: pressing volume parts ratio, successively chooses 5-10 parts of mescenchymal stem cells and freeze peptide solution, 86-94
Part mixed plant extract solution and 1-4 parts of glycerites are put into mixing arrangement, stir evenly obtained homogeneous solution, then
The homogeneous solution is sprayed at facial mask surface, finally facial mask is integrally lyophilized, is made and freezes peptide facial mask.
The beneficial effects of the present invention are: all cytokine antagonist polypeptides that the facial mask secretes mescenchymal stem cell and extraction
The effective components whole high efficiency extraction such as object is simultaneously lyophilized, so that the activity and function of stem cell extract are saved and benefit
With, and the time survived can be longer, is able to extend its function and effect, and by extracting with the centella accordingly matched
Object, german chamomile flower extract, polygonum cuspidate root extract, a kind of reed mentioned in ancient books root extract and glycerite carry out mixing in normal temperature environment and are sprayed at face
In film substrate, frozen dried is carried out, repairs skin so that freezing peptide facial mask and effectively realizing moisturizing, lighten the stain, go wrinkle etc.
Effect.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, below with reference to embodiment pair
The present invention is described in further detail.
Embodiment 1:
The present invention provides a kind of jelly peptide facial masks, freeze peptide solution, 93 by volume parts ratio, including 5 parts of mescenchymal stem cells
Part mixed plant extract solution, 2 parts of glycerites and facial mask substrate, by mass fraction ratio, the mescenchymal stem cell freezes
Peptide solution includes 10 parts of extract freeze-drying powders and 90 parts of distilled water, by mass parts ratio, the mixed plant extract solution
Including 1 part of Gotu Kola P.E, 0.5 part of german chamomile flower extract, 0.5 part of polygonum cuspidate root extract, 0.5 part of a kind of reed mentioned in ancient books root extract and
97.5 parts of distilled water, by mass parts ratio, glycerite includes 40 parts of glycerol and 60 parts of distilled water, and the extract freeze-drying powder is logical
It crosses the culture processing of mescenchymal stem cell starvation to be made, the time of starvation culture is 16 hours, and the mescenchymal stem cell extracts from
The mescenchymal stem cell of Goat Placenta, and cultivated to obtain according to the method normally cultivated, incubation time is 16 hours.
Embodiment 2:
A kind of jelly peptide facial mask, by volume parts ratio, including 10 parts of mescenchymal stem cells freeze peptide solution, 87 parts of mixed plants mention
Object solution, 3 parts of glycerites and facial mask substrate are taken, by mass fraction ratio, it includes 25 that the mescenchymal stem cell, which freezes peptide solution,
Part extract freeze-drying powder and 75 parts of distilled water, by mass parts ratio, the mixed plant extract solution includes 3 parts of centellas
Extract, 2 parts of german chamomile flower extracts, 2 parts of polygonum cuspidate root extracts, 2 parts of a kind of reed mentioned in ancient books root extracts and 91 parts of distilled water, by mass fraction
Than glycerite includes that 60 parts of glycerol and 40 parts of distilled water, the extract freeze-drying powder are cultivated by mescenchymal stem cell starvation
Processing is made, and the time of starvation culture is 16 hours, and the mescenchymal stem cell extracts from the mescenchymal stem cell of Goat Placenta, and
It is cultivated to obtain according to the method normally cultivated, incubation time is 56 hours.
Embodiment 3:
A kind of jelly peptide facial mask, by volume parts ratio, including 8 parts of mescenchymal stem cells freeze peptide solution, 90 parts of mixed plants mention
Object solution, 2 parts of glycerites and facial mask substrate are taken, by mass fraction ratio, it includes 18 that the mescenchymal stem cell, which freezes peptide solution,
Part extract freeze-drying powder and 82 parts of distilled water, by mass parts ratio, the mixed plant extract solution includes 1.8 parts of accumulated snow
Careless extract, 1 part of german chamomile flower extract, 1 part of polygonum cuspidate root extract, 1 part of a kind of reed mentioned in ancient books root extract and 95.2 parts of distilled water, by quality
Portion rate, glycerite include 55 parts of glycerol and 45 parts of distilled water, and the extract freeze-drying powder passes through mescenchymal stem cell starvation
Culture processing is made, and the time of starvation culture is 16 hours, and the mesenchyma that the mescenchymal stem cell extracts from Goat Placenta is dry thin
Born of the same parents, and cultivated to obtain according to the method normally cultivated, incubation time is 56 hours.
According to above-described embodiment, it is preferable that extract freeze-drying powder is placed in -20 DEG C of preservations, and the jelly peptide facial mask facial mask is whole
Body passes through frozen dried.
A kind of preparation method for freezing peptide facial mask, comprising the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and body
Product specific concentration is that 10% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask and is trained under the conditions of 37 DEG C, 5%CO2
It supports, incubation time is 56 hours；
The preparation of extract freeze-drying powder: reaching 70%-80% to growth of mesenchymal stem cells degree, clear using physiological saline
It washes mescenchymal stem cell 2 times, hungry culture 16 hours is carried out to mescenchymal stem cell with Multiple electrolytes injection, it is raw to cell
After long degree reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, cell culture supernatant is collected after the completion of centrifugation and obtains
Stem cell factor is taken, and mescenchymal stem cell extract is extracted using extract extracts kit, wherein mesenchyma is dry
Cell extract is mainly excretion body, then by the stem cell factor of extraction and excretion body be put into freeze dryer after mixing into
Row freeze-drying, forms extract freeze-drying powder, which can save at -20 DEG C for a long time；
Mescenchymal stem cell jelly peptide solution preparation: press mass fraction ratio, weigh 10-25 parts of extract freeze-drying powders and
75-90 parts of distilled water are stirred into mescenchymal stem cell at normal temperature and freeze peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures 1-3 parts of Gotu Kola P.Es, 0.5-2 parts of mothers
Chrysanthemum extract, 0.5-2 parts of polygonum cuspidate root extracts, 0.5-2 parts of a kind of reed mentioned in ancient books root extracts and the 91-97.5 parts of distilled water systems of stirring evenly
；
The preparation of glycerite: pressing mass fraction ratio, weigh 40-60 part glycerol and 40-60 parts of distilled water, general when stirring
Glycerol is dissolved in distilled water, is stirred evenly obtained；
Freeze the preparation of peptide facial mask: pressing volume parts ratio, successively chooses 5-10 parts of mescenchymal stem cells and freeze peptide solution, 86-94
Part mixed plant extract solution and 1-4 parts of glycerites are put into mixing arrangement, stir evenly obtained homogeneous solution, then
The homogeneous solution is sprayed at facial mask surface, finally facial mask is integrally lyophilized, is made and freezes peptide facial mask.
Sensitivity test
The first step test first 100 small white mouse back side shavings and stick embodiment 1-3 proportion generate jelly peptide facial mask,
Continuous detection three days, testing staff observe that the small white mouse back side does not generate the adverse reactions such as red and swollen, acne；Second step test,
The adult human for choosing 60 20-45 one full year of life tests, and 60 testing crews are divided into 3 groups of every group of 20 people, carries out respectively
The product anaphylaxis detection of 3 groups of embodiments is stated, the left face of all testers does not use facial mask, while right face uses facial mask, inspection
Survey personnel detect the facial skin variation of tester at any time, and personnel and testing staff identify the right face of all testers after tested
Without occurring, redness perhaps plays the adverse reactions such as acne therefore the jelly peptide essence will not generate adverse reaction or stimulation to human body
Reaction.
Curative effect test
In view of women for the fine distinguishing ability of cosmetics, therefore choose 27-35 years old, 36-40 years old and 41-45 years old
Each 60 women test, and the women of three groups of age brackets is separated into 3 groups of 20 people's test cells for using embodiment 1-3 respectively,
In use, the left face of tester, using the half facial mask for smearing distilled water, right face uses the facial mask, once a day, each face
Film absorbs half an hour, persistently uses one month, using women and is unaware of the ingredients of two groups of facial masks, then tester distinguishes
Compare left face respectively to the moisturizing for the right face for using the facial mask, lighten the stain, wrinkle three gone to score, A- effect is very aobvious
It writes, B- effect is obvious, and C- effect is general, and to no effect, then staff checks the scoring number of every group of option to D-, obtains such as table
1 test result:
Table 1
By to table 1 data analysis, the jelly peptide facial mask for moisturizing, lighten the stain, go wrinkle have obvious curative effects, for protect
The moistening effect of wet performance, all age group is close, but the moistening effect of embodiment 3 is substantially better than other two groups of embodiments；
For lightening the stain and going wrinkle two, in a certain range with the increase at age, effect is more obvious, and embodiment 3
Effect is substantially better than embodiment 1 and embodiment 2, show that embodiment 3 is most preferred embodiment in conjunction with table 1.
Analysis of cases:
Name: Wang little Jie age: 26 years old skin quality: two cheek peeling of dry hide
Service life: 2-3 times weekly
Effect: moisturizing effect is good, effectively eliminates the skin problem of dry hide and two cheek peelings
Name: the Zhao little Jie age: 32 years old skin quality: mixing dry doubling and skin is dark yellow
Service life: 3 weekly
Effect: facial mask quality do not stick clearly it is greasy, after use without cleaning, turgor is glossy after absorption, have reparation and
Highlight effect
Name: Sun little Jie age: 29 years old skin quality: dry hide sensitivity flesh red capillary
Service life: 3 times a week, preceding use is slept
Effect: to unstable skin releive maintenance the effect of, general red and decortication situation improves obvious
The beneficial effects of the present invention are: all cytokine antagonist polypeptides that the facial mask secretes mescenchymal stem cell and extraction
The effective components whole high efficiency extraction such as object is simultaneously lyophilized, so that the activity and function of stem cell extract are saved and benefit
With, and the time survived can be longer, is able to extend its function and effect, and by extracting with the centella accordingly matched
Object, german chamomile flower extract, polygonum cuspidate root extract, a kind of reed mentioned in ancient books root extract and glycerite carry out mixing in normal temperature environment and are sprayed at face
In film substrate, frozen dried is carried out, repairs skin so that freezing peptide facial mask and effectively realizing moisturizing, lighten the stain, go wrinkle etc.
Effect.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of jelly peptide facial mask, which is characterized in that press volume parts ratio, including 5-10 parts of mescenchymal stem cells freeze peptide solution, 86-
94 parts of mixed plant extract solutions, 1-4 parts of glycerites and facial mask substrate, by mass fraction ratio, the mesenchyma is dry thin
It includes 10-25 parts of extract freeze-drying powders and 75-90 parts of distilled water that born of the same parents, which freeze peptide solution, by mass parts ratio, the mixed plant
Extract solution includes 1-3 parts of Gotu Kola P.Es, 0.5-2 parts of german chamomile flower extracts, 0.5-2 parts of polygonum cuspidate root extracts, 0.5-2
Part a kind of reed mentioned in ancient books root extract and 91-97.5 parts of distilled water, by mass parts ratio, glycerite includes 40-60 parts of glycerol and 40-60 parts
Distilled water, the extract freeze-drying powder are made by the culture processing of mescenchymal stem cell starvation, and the mescenchymal stem cell extracts
It is cultivated to obtain from the mescenchymal stem cell of Goat Placenta, and according to the method normally cultivated.
2. a kind of jelly peptide facial mask according to claim 1, which is characterized in that press volume parts ratio, including 6-8 parts of mesenchymas
Stem cell freezes peptide solution, 88-91 parts of mixed plant extract solutions, 1-3 parts of glycerites and facial mask substrate, by mass fraction
Than it includes 15-22 parts of extract freeze-drying powders and 78-85 parts of distilled water that the mescenchymal stem cell, which freezes peptide solution, by mass parts
Number ratios, the mixed plant extract solution include 1.5-2.5 parts of Gotu Kola P.Es, 0.8-1.8 parts of german chamomile flower extracts,
0.7-1.8 parts of polygonum cuspidate root extracts, 0.7-1.8 parts of a kind of reed mentioned in ancient books root extracts and 92.1-96.3 parts of distilled water, by mass parts ratio,
Glycerite includes that 42-58 parts of glycerol and 42-58 parts of distilled water, the extract freeze-drying powder are trained by mescenchymal stem cell starvation
Processing is supported to be made, the mescenchymal stem cell extracts from the mescenchymal stem cell of Goat Placenta, and according to the method normally cultivated into
Row culture obtains.
3. a kind of jelly peptide facial mask according to claim 2, which is characterized in that mescenchymal stem cell starvation culture when
Between be 12-24 hours.
4. a kind of jelly peptide facial mask according to claim 3, which is characterized in that the incubation time of the mescenchymal stem cell is
48-72 hours.
5. a kind of jelly peptide facial mask according to claim 4, which is characterized in that extract freeze-drying powder is placed in -20 DEG C of preservations, and
And freeze peptide facial mask entirety frozen dried.
6. a kind of preparation method for freezing peptide facial mask, which comprises the following steps:
The culture of mescenchymal stem cell: isolating mescenchymal stem cell from Goat Placenta, with without phenol red DMEM/F12 and volume ratio
Concentration is that 8%-12% fetal calf serum does complete medium, is put into T75 Tissue Culture Flask under the conditions of 35-38 DEG C, 3-6%CO2
It is cultivated, incubation time is 48-72 hours；
The preparation of extract freeze-drying powder: reach 70%-80% to growth of mesenchymal stem cells degree, between cleaning using physiological saline
Mesenchymal stem cells 2-3 times carry out hungry culture 12-24 hours to mescenchymal stem cell with Multiple electrolytes injection, to cell
After extent of growth reaches 90%-95%, centrifugal treating is carried out to interstital stem cell, cell culture supernatant is collected after the completion of centrifugation
Stem cell factor is obtained, and mescenchymal stem cell extract is extracted using extract extracts kit, wherein mesenchyma
Stem cell extract is mainly excretion body, is then put into the stem cell factor of extraction and excretion body in freeze dryer after mixing
It is lyophilized, forms extract freeze-drying powder, which can be in -20 DEG C of long-term preservations；
The preparation of mescenchymal stem cell jelly peptide solution: mass fraction ratio is pressed, 10-25 parts of extract freeze-drying powders and 75-90 are weighed
Part distilled water is stirred into mescenchymal stem cell at normal temperature and freezes peptide solution；
The preparation of mixed plant extract solution: pressing mass parts ratio, measures 1-3 parts of Gotu Kola P.Es, 0.5-2 parts of Flos Chrysanthemis
Extract, 0.5-2 parts of polygonum cuspidate root extracts, 0.5-2 parts of a kind of reed mentioned in ancient books root extracts and 91-97.5 parts of distilled water, which stir evenly, is made mixed
Close plant extract solution；
The preparation of glycerite: pressing mass fraction ratio, weigh 40-60 parts of glycerol and 40-60 parts of distilled water, by glycerol when stirring
It is dissolved in distilled water, stirs evenly obtained；
Freeze the preparation of peptide facial mask: pressing volume parts ratio, successively choose 5-10 part mescenchymal stem cells freeze peptide solutions, 86-94 parts mix
It closes plant extract solution and 1-4 parts of glycerites is put into mixing arrangement, stir evenly obtained homogeneous solution, then should
Homogeneous solution is sprayed at facial mask surface, and finally facial mask is integrally lyophilized, and is made and freezes peptide facial mask.
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| CN201910842715.4A CN110403898A (en) | 2019-09-06 | 2019-09-06 | A kind of jelly peptide facial mask and preparation method thereof |
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| CN201910842715.4A CN110403898A (en) | 2019-09-06 | 2019-09-06 | A kind of jelly peptide facial mask and preparation method thereof |
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| CN201910842715.4A Pending CN110403898A (en) | 2019-09-06 | 2019-09-06 | A kind of jelly peptide facial mask and preparation method thereof |
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| CN115252531A (en) * | 2022-08-11 | 2022-11-01 | 睿博斯(北京)生物科技有限公司 | Anti-wrinkle essence and preparation method thereof |
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Application publication date: 20191105 |