CN107375189A - A kind of stem cell composition and face rejuvenation cosmetic formulation - Google Patents

A kind of stem cell composition and face rejuvenation cosmetic formulation Download PDF

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Publication number
CN107375189A
CN107375189A CN201710318005.2A CN201710318005A CN107375189A CN 107375189 A CN107375189 A CN 107375189A CN 201710318005 A CN201710318005 A CN 201710318005A CN 107375189 A CN107375189 A CN 107375189A
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CN
China
Prior art keywords
stem cell
cell composition
skin
cosmetic formulation
umbilical cord
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Pending
Application number
CN201710318005.2A
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Chinese (zh)
Inventor
马颖
马铎
阮雯莉
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Guangzhou Zisheng Biotechnology Co Ltd
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Guangzhou Zisheng Biotechnology Co Ltd
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Priority to CN201710318005.2A priority Critical patent/CN107375189A/en
Publication of CN107375189A publication Critical patent/CN107375189A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/91—Injection

Abstract

The application belongs to cosmetic field, and in particular to a kind of stem cell composition and face rejuvenation cosmetic formulation.Stem cell composition provided by the present invention includes：Umbilical cord mesenchymal stem cells, sodium hyaluronate, Wushengtai and EGF, compatibility is reasonable, Synergistic so that stem cell composition of the present invention has the function of wrinkle resistance, whitening moisturizing simultaneously, and the time maintained is longer.Above-mentioned stem cell composition is applied to prepare a kind of face rejuvenation cosmetic formulation, facial wrinkles can be eliminated, improve skin texture, bright color, repairing effect is good, length of holding time.

Description

A kind of stem cell composition and face rejuvenation cosmetic formulation
Technical field
The invention belongs to cosmetic field, and in particular to a kind of stem cell composition and face rejuvenation cosmetic formulation.
Background technology
Face rejuvenation (Face rejuvenation) is to refer to make the face of aging to recover the various of rejuvenation appearance Means and method.Facial aging, it is the result that the hard and soft tissue of body and face/neck changes jointly, is mainly shown as：Facial skin Skin is thin and dries, and skin elasticity weakens, and cutaneous pigmentation, colour of skin dimness is uneven, and wrinkle increases, and subcutaneus adipose tissue is reduced Or local accumulation, sagging, muscular atrophy caused by support structure relaxation.Facial aging and constitution, inherent cause, living environment, The factors such as mental emotion, working environment, nutrition condition, disease and topical skin care have close association.Common people arrive Just start aging sign occur after 25 years old, the formation of face skin wrinkle is a key character and index.
At present, it is that a kind of use is relatively broad face is recovered rejuvenation using autologous fat and PRP joint injection faces Beauty means.But because of the own absorption of autologous fat, its repairing effect is limited, and it is shorter to hold time.
The content of the invention
In order to solve this limited technical problem of repairing effect in existing beautifying technique, the invention provides a kind of new Stem cell composition and face rejuvenation cosmetic formulation, the effect of being provided simultaneously with wrinkle resistance, whitening moisturizing, repairing effect is good, maintains Time is grown.
The concrete technical scheme of the present invention is as follows：
The invention provides a kind of stem cell composition, including：Umbilical cord mesenchymal stem cells, sodium hyaluronate, Wushengtai and EGF。
Preferably, the cell density of the umbilical cord mesenchymal stem cells is 5 × 105~5 × 106Individual/mL.
Preferably, the concentration of the Wushengtai is 1~5mg/mL.
Preferably, the concentration of the sodium hyaluronate is 35vol%~60vol%.
Preferably, the concentration of the EGF is 10~50mg/mL.
Present invention also offers a kind of face rejuvenation cosmetic formulation of wrinkle resistance, whitening moisturizing, is combined comprising above-mentioned stem cell Acceptable auxiliary material on thing and pharmacy.
Preferably, the formulation of the face rejuvenation cosmetic formulation is ejection preparation, aqua, gel or creme.
In summary, the present invention using umbilical cord mesenchymal stem cells joint sodium hyaluronate use, and add simultaneously Wushengtai and EGF obtains a kind of stem cell composition, applied to skin wrinkle resisting and improves skin texture and the colour of skin.Umbilical cord mesenchymal stem cells Breeding under the regulation and control of body and secreting substantial amounts of active factors improves skin, moreover it is possible to it is thin to be differentiated to form adipose tissue filling face Line；Wushengtai can promote collagen, elastic fibers and hyaluronic acid synthesis, improve the water content of skin, increase skin thickness And microgroove is reduced, beneficial to the reparation of damage skin or inflammation skin, skin energy balance is kept, so as to avoid skin because of grease Melanin is excessively calm caused by content is low, cuticula is thinning, strengthens whitening effect；Sodium hyaluronate and EGF can improve skin rapidly The water content of skin, maintain skin moisturizing smooth, fine and smooth tender and high resilience, there is wrinkle resistant, crease-resistant, beauty and health care and recover skin The effect of skin physiological function.Above-mentioned each component compatibility is reasonable, Synergistic so that stem cell composition of the present invention has anti-simultaneously The function of wrinkle whitening and moisturizing, and the time maintained is longer.Above-mentioned stem cell composition is applied to cosmetic field, one can be used as The new face rejuvenation cosmetic formulation of kind, eliminates facial wrinkles, improves skin texture, and bright color, its repairing effect is good, dimension Hold time length.
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this The embodiment of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can also basis The accompanying drawing of offer obtains other accompanying drawings.
Fig. 1-1a, Fig. 1-2 a, Fig. 1-3a and Fig. 1-4a are that the umbilical cord mesenchymal stem cells cell phenotype of blank control group is special The qualification result of sign；
The cell for the umbilical cord mesenchymal stem cells that Fig. 1-1b, Fig. 1-2 b, Fig. 1-3b and Fig. 1-4b are separately cultured to obtain for embodiment 1 The qualification result of phenotypic characteristic.
In order to provide the face rejuvenation cosmetic formulation that a kind of repairing effect is good, holds time long, the invention provides one Kind stem cell composition and face rejuvenation cosmetic formulation.
The present invention is not particularly limited to the Wushengtai in stem cell composition, sodium hyaluronate and EGF source, using ability Commercially available prod known to field technique personnel, or prepared using this area routine techniques.Following examples In EGF be the obtained EGF solution of EGF freeze-dried powders and solvent mixed dissolution, buy in Kang Jie bioengineering Co., Ltd；Five Victory peptide is bought in Jin Sirui bio tech ltd；Sodium hyaluronate is bought in Guangzhou Run Chuyan biological medicines Science and Technology Ltd..
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made Embodiment, belong to the scope of protection of the invention.
The umbilical cord mesenchymal stem cells of embodiment 1 are separately cultured
1st, the umbilical cord of health full term surgical neonate is taken, umbilical cord amniotic membrane is peeled off after sterile cleaning is carried out in super-clean bench and moves Vein, take out jelly of Wharton；Shredded with operating scissors, add DMEM in high glucose+10%FBS complete mediums and be resuspended, be then inoculated with Into 15cm plates, 5%CO is transferred to2, cultivate in 37 DEG C of environment.Often cultivate fluid infusion in 5 days once, until cell climbs out of, abandon Tissue block is removed, new complete medium is supplemented and continues to cultivate, treats that cell forms larger clone, carries out Secondary Culture.
2nd, the P0 that can be passed on is taken to discard original fluid in ware for cell, add 10mLPBS buffer solutions and gently rinse cell life Long face, discard washing lotion.Then, the trypsase of 2mL 0.25% is added, the plate that rolls makes pancreatin uniform fold in ware bottom；In Micro- Microscopic observation visible cell gap increase, kytoplasm retraction, when observing that spindle shape cell rounding brightens, adds 10 immediately The complete medium of times volume terminates digestion.Then, blow and beat cell repeatedly with liquid-transfering gun, until cell all come off into it is unicellular Suspension is transferred in centrifuge tube after suspension, 300rpm centrifuges 5min at room temperature.Then, DMEM+ is added after removing supernatant Cell is resuspended in 10%FBS complete mediums, by 1 × 105Individual/mL cell density inoculation, be placed in 37 DEG C, it is 5% carbon dioxide, full With Secondary Culture is carried out in the cell culture incubator of humidity.Finally, P2 is collected respectively to the umbilical cord mesenchymal stem cells in P5 generations, is treated With.
3rd, the identification of the cell phenotype feature of umbilical cord mesenchymal stem cells
P3 is taken to use normal saline dilution to obtain density as 1 × 10 for umbilical cord mesenchymal stem cells6Individual/mL stem cell Suspension, anti-CD105, CD73, CD34, CD45 each 5 μ L of monoclonal antibody are drawn respectively, add the μ L of cell suspension 500, at room temperature Lucifuge is incubated 20min, while sets up blank Isotype control group；1500prm centrifuges 5min, supernatant is abandoned, with the PBS containing 10%FBS Washing 2 times, then above machine testing is resuspended after sieving with 500 μ L PBS.Fig. 1 is qualification result.
Embodiment 2
Tri- kinds of face rejuvenation cosmetic formulations of A, B and C, its specific preparation process are prepared respectively according to the formula described in table 1 It is as follows：
1st, the preparation of Wushengtai mother liquor：Wushengtai powder is weighed, adds physiological saline, dissolving, it is 500mg/ to obtain concentration ML Wushengtai mother liquor, with 0.22 μm of membrane filtration, it is stand-by to be placed in 4 DEG C of refrigerators.
2nd, the preparation of EGF solution：One bottle of EGF freeze-dried powder and one bottle of solvent are taken out respectively, are drawn with disposable syringe molten Matchmaker, squeeze into EGF and freeze in powder bottle, fully mix, form 2.5mL EGF solution.
3rd, toward sodium hyaluronate is added in EGF solution, A liquid is obtained；Filled between the umbilical cord prepared in embodiment 1 is resuspended with physiological saline Matter stem cell, umbilical cord mesenchymal stem cells suspension is obtained, then add in step 1 the Wushengtai mother liquor prepared, obtain B liquid； Finally, A liquid and B liquid are mixed, are then transferred in syringe, face rejuvenation cosmetic formulation A, B and C is made.
Table 1
Stem cells density (individual/mL) Wushengtai (g/mL) EGF(μg/mL) PRP (vol%)
Preparation A 5×105 5 50 20
Preparation B 1×106 3 15 10
Formulation C 5×106 1 30 30
3 facial anti-wrinkle effect of embodiment is evaluated
Choosing 120 faces has the normal adults (men and women half and half) of obvious wrinkle, and age bracket is 40~60 years old；Random point Into 4 groups, including 3 groups of control groups and 1 group of experimental group, preparation as shown in table 2 is then injected to facial wrinkles compared with many places respectively Corium deep layer, evaluation anti-wrinkle effect after 4 weeks.Its evaluation index is：Degree, which is repaired, according to facial wrinkles carries out cosmetic result Scoring, it is better to repair, and scoring is higher；Hold time, be from the time span for cosmetic result occur and being lost to cosmetic result.Such as Shown in the result of table 3, embodiment 1 is far above control group 1 to 3 in wrinkle repairing effect and aspect of holding time, and shows that its is good Cosmetic result.
Table 2
Group Component
Control group 1 Physiological saline
Control group 2 Umbilical cord mesenchymal stem cells+physiological saline
Control group 3 Umbilical cord mesenchymal stem cells+PRP+ physiological saline
Experimental group Preparation A
Table 3
Control group 1 Control group 2 Control group 3 Experimental group
Average crease-resistant scoring 10.14±0.69 69.36±1.58 74.49±2.49 86.47±4.03
Averagely hold time (moon) / 12.4±0.8 19.53±0.7 24.13±0.9
The skin colorimetric detection of embodiment 4
Using the change of Lab colorimeter systems spectrophotometer (Minolta spectrophotometer) measurement skin color.Light source selects With the X- of standard illuminants (typical daylight)-D65,1931CIE-RGB system standard observer's spectral tristimulus value 0.3127.Y -0.3291, correlated colour temperature 6504, with D65/100Mode output data.
1 day before the injection, detect the whitening degree of skin.1st month, the 2nd month and 3rd month point after ejection preparation Not Shi Yong spectrophotometer measurement detection skin brightness value, average, evaluate whitening effect.As shown in the result of table 4, experimental group It is better than control group 1~3 in skin whitening effects and aspect of holding time, shows its good cosmetic result.
Table 4
Control group 1 Control group 2 Control group 3 Experimental group
Before experiment 63.42±3.89 64.16±3.25 63.68±3.57 64.58±4.25
1st month 63.33±3.07 65.18±2.73 67.35±2.42 72.64±3.37
2nd month 63.56±3.66 69.02±2.59 68.93±3.76 73.03±2.35
3rd month 64.28±2.25 68.45±3.14 71.04±2.27 74.38±2.24
The moisture of skin of embodiment 5 detects
Use the water content of skin moisture content tester Corneometer CM825 (German CK companies) test skins MMV values.In the first 1 day MMV value for detecting skin of injection, 1st month, the 2nd month and 3rd month then after injection makes Moisture content of skin is detected with spectrum photometer measurement, is averaged, evaluates Water-saving effect.As can be seen from the results of table 5, experimental group is in skin Skin Water-saving effect and aspect of holding time are better than control group 1~3, show its good cosmetic result.
Table 5
Control group 1 Control group 2 Control group 3 Experimental group
Before experiment 19.47±1.68 19.57±1.25 20.48±1.26 20.79±1.53
1st month 20.14±1.47 46.85±1.63 50.24±1.35 60.25±1.44
2nd month 20.75±1.59 47.26±1.75 49.53±1.66 62.74±1.37
3rd month 21.03±1.45 47.47±1.89 49.79±1.83 62.56±1.28

Claims (7)

  1. A kind of 1. stem cell composition, it is characterised in that including：Umbilical cord mesenchymal stem cells, sodium hyaluronate, Wushengtai and EGF.
  2. 2. stem cell composition according to claim 1, it is characterised in that the cell of the umbilical cord mesenchymal stem cells is close Spend for 5 × 105~5 × 106Individual/mL.
  3. 3. stem cell composition according to claim 1, it is characterised in that the concentration of the Wushengtai is 1~5mg/mL.
  4. 4. stem cell composition according to claim 1, it is characterised in that the concentration of the sodium hyaluronate be 35vol%~ 60vol%.
  5. 5. stem cell composition according to claim 1, it is characterised in that the concentration of the EGF is 10~50 μ g/mL.
  6. 6. a kind of face rejuvenation cosmetic formulation, it is characterised in that include the stem cell described in claim 1 to 5 any one Acceptable auxiliary material on composition and pharmacy.
  7. 7. face rejuvenation cosmetic formulation according to claim 6, it is characterised in that its formulation be ejection preparation, aqua, Gel or creme.
CN201710318005.2A 2017-05-08 2017-05-08 A kind of stem cell composition and face rejuvenation cosmetic formulation Pending CN107375189A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110368321A (en) * 2018-04-11 2019-10-25 上海坤福生物科技有限公司 A kind of biological beauty product in human stem cell source and preparation method thereof
CN111317677A (en) * 2018-12-13 2020-06-23 田娟 Human stem cell cosmetic formula with repairing effect on damaged cells
CN115444805A (en) * 2022-09-27 2022-12-09 广东金专生物科技有限公司 Stem cell facial beauty composition and application thereof

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Publication number Priority date Publication date Assignee Title
CN105055187A (en) * 2015-09-14 2015-11-18 广州赛莱拉干细胞科技股份有限公司 Facial rejuvenation cosmetic preparation and preparation method for same
CN105056303A (en) * 2015-09-21 2015-11-18 广州赛莱拉干细胞科技股份有限公司 Composition, preparation and application thereof
CN106377474A (en) * 2016-10-09 2017-02-08 倪飞鹏 Dendrobium officinale mask and manufacturing method thereof

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CN105055187A (en) * 2015-09-14 2015-11-18 广州赛莱拉干细胞科技股份有限公司 Facial rejuvenation cosmetic preparation and preparation method for same
CN105056303A (en) * 2015-09-21 2015-11-18 广州赛莱拉干细胞科技股份有限公司 Composition, preparation and application thereof
CN106377474A (en) * 2016-10-09 2017-02-08 倪飞鹏 Dendrobium officinale mask and manufacturing method thereof

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110368321A (en) * 2018-04-11 2019-10-25 上海坤福生物科技有限公司 A kind of biological beauty product in human stem cell source and preparation method thereof
CN111317677A (en) * 2018-12-13 2020-06-23 田娟 Human stem cell cosmetic formula with repairing effect on damaged cells
CN115444805A (en) * 2022-09-27 2022-12-09 广东金专生物科技有限公司 Stem cell facial beauty composition and application thereof
CN115444805B (en) * 2022-09-27 2023-08-22 广东金专生物科技有限公司 Stem cell facial cosmetic composition and application thereof

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Application publication date: 20171124