CN105030627A - Salicornia herbacea cell face cream and preparation method thereof - Google Patents
Salicornia herbacea cell face cream and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an anti-aging skin care product and particularly discloses a salicornia herbacea cell face cream and a preparation method thereof. The salicornia herbacea cell face cream includes the following components: 1) a group A: 1-3% of cetyl alcohol, 0.2-2% of octadecanol, 5-15% of squalane, 1-3% of stearic acid, 0.2-2% of glycerin monostearate, 1-3% of Vaseline and 5-15% of wool fat; and 2) a group B: 5-15% of stem cell extract, 0.2-2% of silk peptide, 1-3% of collagen, 2-7% of glycerin, 0.2-0.7% of lactic acid, 0.2-2% of hyaluronic acid and 0.2-2% of a preservative. All the components are added with deionized water to 100%. The preparation method includes the steps of (1) mixing the components in the group A, heating the components to 70-75 DEG C; (2) heating the deionized water to 70-75 DEG C; (3) adding the group A to the deionized water, mixing the components uniformly, and stirring the mixture and decreasing the temperature; and (4) when the temperature is reduced to lower than 40 DEG C, adding the components in the group B and mixing the components uniformly. The salicornia herbacea cell face cream has excellent effects on improving skin elasticity, reducing wrinkles and keeping the skin to be smooth and tender.
Description
Technical field
The present invention relates to a kind of antidotal skin care item, be specially grass green salicornia europaeal extract cell facial cream and preparation method thereof.Belong to cosmetic technical field.
Background technology
At the cosmetics of skin anti-aging, there is multiple method, as by the formula such as hormone, plant extract, wrinkle removing process is carried out to skin, but these methods can not fundamentally reach antidotal effect, Corticosteroids has harm to human body, and can only obtain short run effect, life-time service aggravation skin aging, and the DeGrain of plant extract, need life-time service.
Along with improving constantly of living standard, the maintenance of people to health especially skin more and more comes into one's own.Skin histology is human body maximum tissue organ, covers human body surface, intercepts the detrimental effect that all harmful substances of nature produce viable organism.But along with the increase at age, the function of skin histology organ is aging gradually, from aesthetic view point, have a strong impact on the enthusiasm of people to life; From physiological angle, affect the available protecting of human internal organs.The main cause of skin aging is, the various quantity of nutrient matter needed in metabolic processes and quality can not obtain physiological and meet from diet, thus cause the quantity of the synthesis material of the life hereditary material carrier DNA in life cells core to reduce gradually, it is finally caused to adjust the kinds of protein of empty synthesis and quantity to reduce, cellular metabolic activity declines thereupon, and the life-span shortens relatively.The performance of the excessive aging phenomenon of skin is: cutis laxa, wrinkle increase degradation under intensification, pore increase, the thicker turmoil of skin texture, skin elasticity.At present, on the market for the most moisturizing of product of skin protection, limited efficacy.
Discarded umbilical cord when Human Umbilical Cord's mescenchymal stem cell (humanumbilicalmesenchymalstemcells, HUMSC) and placenta stem-cell (placentalstemcell, PSC) derive from Delivery and placenta tissue.Umbilical cord includes two umbilical arterys, a umbilical vein, containing special embryo's mucoid connective tissue-Wal Tong Shi glue (WhartonpsJelly) between arteriovenous, be separated from Wal Tong Shi glue the stromal cell obtained and be human umbilical cord mesenchymal stem cells (humanumbilicalmesenchymalstemcell, HUMSC).Placenta Hominis is that fetus draws the place of nutrition from parent, is divided into rete layer, tapetum and de-basement membrane layer.HUMSC and PSC has multi-lineage potential and plasticity, the histiocyte in three germinal layer sources can be divided into, as osteoblast, chondrocyte, adipose cell, islet cells, neurocyte, myocardial cell, sexual cell (as spermatogonium, oocyte etc.), and abundant trophic factors can be secreted.These abundant trophic factors not only can the aging of delaying skin stem cell, can also promote epidermal stem cells and hair follicle stem cells propagation, have important function to maintenance skin elasticity and health.
Summary of the invention
For above-mentioned technical problem, the invention provides the short and anti-aging skin care product had no side effect of a kind of successful, curative effect, concrete technical scheme is:
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 70-75 degree.Deionized water is heated to 70-75 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
An inventive point of the present invention is exactly different with prior art in the past, after liposoluble substance and surfactant individual processing, the method mixed with other active princlples again, raising like this for the character of final products is very large, be not easy to separate out water, belong to the creative inventive point of originality of the present invention.
Wherein, preferably, method and weight ratio as follows
A group:
B group:
A group is mixed, is heated to 72 degree.Deionized water is heated to 72 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Above-mentioned cell extract is stem cell extract.
Stem cell extract of the present invention is preferably plant stem cell extract;
Plant stem cell (Plantstemcell) includes all formulas about development of plants and growth, has the cell (immortalcell) of eternal life power, is the root (origin) of plant life power.Plant stem cell is present in and is called as in merismatic special tectonic, has very surprising regeneration capacity.These make plant constantly can grow between centuries, and generate brand-new organ." nature " 415, what 715-754 (2002) the present invention adopted is commercially available dried apple slices cell, Tomato Dry cell and dried Fructus Vitis viniferae cell, but in order to more suit goal of the invention of the present invention, inventor is through test of many times, dried apple slices cell is processed and adjusts, make better effects if of the present invention, concrete method is as follows:
According to part by weight 1: 1: 1: 2 by dried apple slices cell, Tomato Dry cell, dried Fructus Vitis viniferae cell and vitamin C mixing;
Conventional method In vitro culture also collects culture;
Collect supernatant middle-molecular-weihydroxyethyl to meet the composition of 5000Da, carry out lyophilization, wherein, cryodesiccated method is as follows: 0 degree Celsius of 5-10 minute ,-10 degrees Celsius of 10-20 minute,-20 degrees Celsius of 2-3 hour, then-35 degrees Celsius of preservations were more than 5 hours, carried out lyophilization, and this is that an inventive point of the present invention have passed through this method, cell active substance can be kept to greatest extent, to the effect of final products, there is extraordinary facilitation.
Stem cell extract of the present invention is preferably placenta stem-cell extract;
The method obtaining stem cell of the present invention is as follows:
(1) collection of umbilical cord and Placenta Hominis.Select mature ewe, Viral diagnosis, aseptically obtain sheep Cord blood and Placenta Hominis, under 0 degrees celsius, be transported to GMP workshop, put into liquid nitrogen container and preserve.(2), after collecting enough Placenta Hominiss and sheep Cord blood, prepare to produce and processing.On ice, with PBS, umbilical cord and Placenta Hominis are rinsed twice, cut off separately, the piece of tissue of 0.5-3mm3 size is cut into after removing blood vessel and adventitia, with tissue refiner, umbilical cord and Placenta Hominis are made tissue homogenate, remove after centrifugal 2-20 minute for 800-900 rev/min and organize relic, supernatant is placed in refrigerator.Preferably, can by this supernatant according to 1: 1 part by weight join in stem cell extract and be integrally used in product of the present invention.
Wherein, described stem cell method for preparing extractive is:
1), the fragmentation of sheep cord blood stem cell: by sheep cord blood stem cell suspension 50 milliliters, adopt ultrasonic fragmentation, utilize ultrasonic oscillator to launch 9 ~ 10 kilocycle sonicated 3-9 minute;
2), in solution, add 10 times of mL normal saline after, 3000 revs/min, centrifugal 20 minutes;
3), get supernatant, add 20% dehydrated alcohol, mixing, places 15 minutes (ethanol precipitation);
4), by solution 3000 revs/min, centrifugal 20 minutes;
5), abandon supernatant, get precipitation, add normal saline 50 milliliters, shake up gently, resolution of precipitate is become clear solution, be sheep cord blood stem cell activated protein crude extract;
Wherein, the preparation method of grass green salicornia europaeal (SALICORNIAHERBACEA) extract is:
Wherein, grass green salicornia europaeal method for preparing extractive is: be 1 to be mixed with deionized water by grass green salicornia europaeal than the weight ratio of 8-10 according to weight ratio, the temperature remaining on 0-4 DEG C soaks 2 hours to 24 hours, broken grass green salt to suspension, then according to 0.2-0.4 liter/kilogram ratio add chitosan; Adding mass ratio is again 3% ~ 6% ethanol, leaves standstill 2 hours; Get 109AB-8 macroporous resin dress 5 parts, post, under PH6-7, extract concentration gets 50ml upper prop 0.1-0.2 gram often liter time, and loading flow velocity is 2BV/h, first with deionized water clarification, then obtains extract with 3BV50% ethanol according to the flow velocity eluting of 2BV/h.Wherein, the better method of extraction effect adds the wintergreen oil with weight portions such as grass green salicornia europaeals, this is that the present invention finds through test of many times, study through inventor, wintergreen oil is water miscible, can the further stripping by the water-soluble components in grass green salicornia europaeal.This is because wintergreen oil has the effect of the irritant reaction such as cutaneous vasodilation, the colour of skin be rubescent, synergism is produced when being that grass green salicornia europaeal is coefficient, product of the present invention can be made further to make skin absorb, change human body skin state rapidly.In addition, the present inventor is surprised to find, time grass green salicornia europaeal extract is used alone, effect is not very good, but it is but very good with the mixed effect of stem cell extract, find through independent stem cell batching, the cosmetics of grass green salicornia europaeal batching and the contrast of both cosmetics used in combination, in skin elasticity, wrinkle minimizing and skin light are tender, there is very large progress.
Stem cell facial cream provided by the invention, from the angle of cell, supplements the function of competent cell or the existing cell of activation targetedly.In the cell that skin is numerous, skin progenitor cell is that various cell turnover is regenerated " seed cell ", is the germinal cell that can produce all kinds cell.Stem cell facial cream, by after epidermal absorption, is deep into skin corium and subcutaneous tissue, differentiation of stem cells in full activation skin, upgrades rapidly and replaces old and feeble cell.Thus increase collagen secretion, improve skin of face elasticity; Promote the synthesizing activity of albumen, strengthen skin of face pliability; Stimulate muscle layer to produce fibroblast and skin protein, increase face skin degree of compacting and elasticity thereof, strengthen skin elasticity, make skin tight.Engulf pigment removal mottle; Help and smooth microgroove, wrinkle, reinvent face mask, make skin become compacting, tender, fine and smoothly to moisten, reduce wrinkle, make overall skin present young state.
Detailed description of the invention
The specific embodiment of the present invention is described in conjunction with the embodiments.
Embodiment 1
Method and weight ratio as follows
A group:
B group:
A group is mixed, is heated to 72 degree.Deionized water is heated to 72 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1: 1, adding mass ratio is again 6% gelatin solution, gelatin solution is 0.3 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
The preparation method of the active extract of described Placenta Hominis is as follows: get fresh Placenta caprae seu ovis, clean and remove fascia, weigh after shredding, carry out tissue grinder, add the normal saline of Placenta caprae seu ovis weight 1 ~ 2 times, carry out high speed tissue homogenate, the Placenta Hominis after homogenate is uniform meat paste shape, then Placenta caprae seu ovis homogenate is dissolved after freezing 48 hours at-30 DEG C ~-40 DEG C at 25 DEG C, so repeatedly freeze-thaw 2 ~ 3 times.Again through 5000 ~ 70000r/min frozen centrifugation 25 ~ 30 minutes, get the static dialysis of supernatant 24 ~ 48 hours or vacuum dialysis can obtain the active extract of Placenta Hominis for 10 ~ 12 hours.
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Embodiment 2
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 75 degree.Deionized water is heated to 75 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Embodiment 3
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 73 degree.Deionized water is heated to 73 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1.2: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 0.8: 1, adding mass ratio is again 5% gelatin solution, gelatin solution is 0.3 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Embodiment 4
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 70-75 degree.Deionized water is heated to 70-75 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Embodiment 5
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 71 degree.Deionized water is heated to 71 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Another one inventive point of the present invention is also, be configured with a kind of natural plants mixed liquor, before emulsifying, after it is added according to the ratio of weight portion 7%, homogenizing and emulsifying is carried out together with other raw materials, wherein, the preparation method of described natural plants mixed liquor is: by Rhizoma Zingiberis Recens, Herba Menthae, after Herba Erodii (HERBAERODII/HERBAGERANII) 1: 1: 1 mixing by weight (preferably before mixing, whole component being dried to water content is 1-3%), rub with machinery respectively, squeeze out juice, residue joins in ethanol water, stir after 2 hours, cross elimination residue, filtrate and juice are mixed to get extracting solution, to be ethanol with water volume ratio be ethanol water 0.5-0.8: 1 mixes and make, the weight ratio of residue and ethanol water is 1: 1.Because of the effect that natural plants mixing composition for this reason has sterilization, renders palatable, make this product under the prerequisite not adding antiseptic, also shelf-life longer time can be had, because the heterotrophia of product of the present invention enriches, so it is also industrially very useful for adding this component.
Embodiment 6
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 72 degree.Deionized water is heated to 72 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Another one inventive point of the present invention is also, be configured with a kind of natural plants mixed liquor, before emulsifying, after it is added according to the ratio of weight portion 3%, homogenizing and emulsifying is carried out together with other raw materials, wherein, the preparation method of described natural plants mixed liquor is: by Rhizoma Zingiberis Recens, Herba Menthae, after Herba Erodii (HERBAERODII/HERBAGERANII) 1: 1: 1 mixing by weight (preferably before mixing, whole component being dried to water content is 1-3%), rub with machinery respectively, squeeze out juice, residue joins in ethanol water, stir after 2 hours, cross elimination residue, filtrate and juice are mixed to get extracting solution, to be ethanol with water volume ratio be ethanol water 0.5-0.8: 1 mixes and make, the weight ratio of residue and ethanol water is 1: 1.Because of the effect that natural plants mixing composition for this reason has sterilization, renders palatable, make this product under the prerequisite not adding antiseptic, also shelf-life longer time can be had, because the heterotrophia of product of the present invention enriches, so it is also industrially very useful for adding this component.
The temperature remaining on 0-4 DEG C soaks 2 hours to 24 hours, broken grass green salt to suspension, then according to 0.2-0.4 liter/kilogram ratio add chitosan; Adding mass ratio is again 3% ~ 6% ethanol, leaves standstill 2 hours; Get 109AB-8 macroporous resin dress 5 parts, post, under PH6-7, extract concentration gets 50ml upper prop 0.1-0.2 gram often liter time, and loading flow velocity is 2BV/h, first with deionized water clarification, then obtains extract with 3BV50% ethanol according to the flow velocity eluting of 2BV/h; Wherein said grass green salicornia europaeal method for preparing extractive is: more preferably, adds the wintergreen oil with weight portions such as grass green salicornia europaeals when soaking grass green salicornia europaeal with deionized water
Embodiment 7
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 70 degree.Deionized water is heated to 70 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Another one inventive point of the present invention is also, be configured with a kind of natural plants mixed liquor, before emulsifying, after it is added according to the ratio of weight portion 5%, homogenizing and emulsifying is carried out together with other raw materials, wherein, the preparation method of described natural plants mixed liquor is: by Rhizoma Zingiberis Recens, Herba Menthae, after Herba Erodii (HERBAERODII/HERBAGERANII) 1: 1: 1 mixing by weight (preferably before mixing, whole component being dried to water content is 1-3%), rub with machinery respectively, squeeze out juice, residue joins in ethanol water, stir after 2 hours, cross elimination residue, filtrate and juice are mixed to get extracting solution, to be ethanol with water volume ratio be ethanol water 0.5-0.8: 1 mixes and make, the weight ratio of residue and ethanol water is 1: 1.Because of the effect that natural plants mixing composition for this reason has sterilization, renders palatable, make this product under the prerequisite not adding antiseptic, also shelf-life longer time can be had, because the heterotrophia of product of the present invention enriches, so it is also industrially very useful for adding this component.
The temperature remaining on 0-4 DEG C soaks 2 hours to 24 hours, broken grass green salt to suspension, then according to 0.2-0.4 liter/kilogram ratio add chitosan; Adding mass ratio is again 3% ~ 6% ethanol, leaves standstill 2 hours; Get 109AB-8 macroporous resin dress 5 parts, post, under PH6-7, extract concentration gets 50ml upper prop 0.1-0.2 gram often liter time, and loading flow velocity is 2BV/h, first with deionized water clarification, then obtains extract with 3BV50% ethanol according to the flow velocity eluting of 2BV/h; Wherein said grass green salicornia europaeal method for preparing extractive is: more preferably, adds the wintergreen oil with weight portions such as grass green salicornia europaeals when soaking grass green salicornia europaeal with deionized water
Embodiment 8
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 75 degree.Deionized water is heated to 75 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
Wherein, described stem cell method for preparing extractive is: the Cord blood gathering sheep adds anticoagulant, and remain on the temperature of 4 DEG C, with normal saline dilution, sheep Cord blood and normal saline volume ratio are 1: 1;
Sheep Cord blood after dilution is joined in the active extract of Placenta Hominis, the volume ratio of the sheep Cord blood of dilution and the active extract of Placenta Hominis is 1.2: 1, adding mass ratio is again 3% gelatin solution, gelatin solution is 0.5 with the volume ratio of the active extract of Placenta Hominis: 1. carry out gradient centrifugation and be separated, and obtains liquid phase interface cell suspension;
Cell suspension phosphate buffer carries out washing twice obtaining stem cell;
Stem cell after washing is put into 1640 culture medium and measures active, and it is 95% that is qualified that activity is greater than;
Qualified stem cell uses hydrodynamic shear to cut into super small-molecule active substance to stem cell.
Be configured with a kind of natural plants mixed liquor, before emulsifying, after it is added according to the ratio of weight portion 7%, homogenizing and emulsifying is carried out together with other raw materials, wherein, the preparation method of described natural plants mixed liquor is: by Rhizoma Zingiberis Recens, Herba Menthae, after Herba Erodii (HERBAERODII/HERBAGERANII) 1: 1: 1 mixing by weight (preferably before mixing, whole component being dried to water content is 1-3%), rub with machinery respectively, squeeze out juice, residue joins in ethanol water, stir after 2 hours, cross elimination residue, filtrate and juice are mixed to get extracting solution, to be ethanol with water volume ratio be ethanol water 0.5-0.8: 1 mixes and make, the weight ratio of residue and ethanol water is 1: 1.Because of the effect that natural plants mixing composition for this reason has sterilization, renders palatable, make this product under the prerequisite not adding antiseptic, also shelf-life longer time can be had, because the heterotrophia of product of the present invention enriches, so it is also industrially very useful for adding this component.
The temperature remaining on 0-4 DEG C soaks 2 hours to 24 hours, broken grass green salt to suspension, then according to 0.2-0.4 liter/kilogram ratio add chitosan; Adding mass ratio is again 3% ~ 6% ethanol, leaves standstill 2 hours; Get 109AB-8 macroporous resin dress 5 parts, post, under PH6-7, extract concentration gets 50ml upper prop 0.1-0.2 gram often liter time, and loading flow velocity is 2BV/h, first with deionized water clarification, then obtains extract with 3BV50% ethanol according to the flow velocity eluting of 2BV/h; Wherein said grass green salicornia europaeal method for preparing extractive is: more preferably, adds the wintergreen oil with weight portions such as grass green salicornia europaeals when soaking grass green salicornia europaeal with deionized water
Effect detection
By normal individual trial test, the antidotal effect of stem cell facial cream that the present invention passes through is described: select the stem cell facial cream obtained by embodiment.
Subjects is selected: each half of men and women, 25 ~ 65 years old every ten years old is an age bracket, is divided into four age brackets, each men and women of each age bracket one, healthy, without dermatosis and allergies.
Test number: 160 people, is divided into 8 groups according to sex is identical with age bracket is close, often organizes 20 people.
Evaluation index: canthus, under-eye skin elasticity, degree of hydration, transdermal dehydration, wrinkle, pore, skin smoothness.
Test zone: face, comprises canthus, now region.
Test period: before using and after using 14 days.
Skin elasticity is tested: skin elasticity is also one of important symbol judging skin aging, and test position is selected in face.Because eye part skin is the thinnest, do not have sebum secretion, and eye motion is frequent, other position more facial is easily old and feeble, therefore tests now.Because canthus also easily produces wrinkle, canthus is also for mainly to detect position.Use MPA580 elasticity tester, execute a constant negative pressure at canthus and under-eye skin place, by the size of testing the reply deformation of skin investigate make to apply some make up after the change of skin elasticity, evaluate the senile-resistant efficacy of cosmetics.
Elastic mensuration index be R2, R2=(e (a)-e (a+b)/e (a): namely without skin during negative pressure springback capacity and have a negative pressure time the ratio of maximum tension amount, ratio, more close to 1, illustrates that skin elasticity is better.Visible by front and back test comparison, skin elasticity all has obvious improvement.
Table 1 skin elasticity test chart
| Tested object numbering group | Use front elasticity measurement index | Use elastic mensuration index after 14 days |
| 1 | 0.656 | 0.887 |
| 2 | 0.867 | 0.943 |
| 3 | 0.687 | 0.832 |
| 4 | 0.743 | 0.976 |
| 5 | 0.845 | 0.986 |
| 6 | 0.764 | 0.843 |
| 7 | 0.854 | 0.976 |
| 8 | 0.732 | 0.932 |
Moisture measurement: by the mensuration to moisture of skin. not only can be directly acquainted with the situation of epiderm skin horny layer moisture content, also indirectly can reflect the degree of skin aging.Moisture measurement is selected in the trigonum of decree stricture of vagina, investigates the change that object of exerting pressure uses degree of hydration before and after stem cell facial cream, evaluates the antidotal effect of cosmetics.It is that skin is drier that degree of hydration is less than 50, and 50 ~ 60 is dry, and being greater than 60 is that moisture is abundant.Visible by front and back test comparison, skin is moisture all has obvious improvement, and major part can reach the sufficient degree of moisture.
Table 2 skin elasticity test chart
| Tested object numbering group | Use front degree of hydration | Use degree of hydration after 14 days |
| 1 | 52 | 65 |
| 2 | 45 | 61 |
| 3 | 56 | 62 |
| 4 | 50 | 65 |
| 5 | 63 | 66 |
| 6 | 38 | 56 |
| 7 | 47 | 66 |
| 8 | 59 | 64 |
Wrinkle ready visual contrast: choosing is analyzed area to the whole cheek at canthus now, investigates experimenter and uses the visual sense organ of wrinkle before and after stem cell facial cream, evaluate the senile-resistant efficacy of cosmetics.Effective by estimating the elimination of visible wrinkle.
Table 3 wrinkle of skin range estimation table
| Tested object numbering group | Wrinkle after 14 days is used whether obviously to eliminate |
| 1 | Not obvious |
| 2 | Clearly |
| 3 | More obvious |
| 4 | Obviously |
| 5 | More obvious |
| 6 | More obvious |
| 7 | More obvious |
| 8 | Obviously |
Skin smoothness: by the change of regular period skin smoothness after mensuration use stem cell facial cream, and facial smoothness contrast before and after using, evaluate the senile-resistant efficacy of cosmetics intuitively, quantitatively.Smoothness evaluation is that the peak dot/valley point by calculating epidermis realizes, close to 1, peak dot/valley point ratio more illustrates that skin is more smooth.Visible by front and back test comparison, skin smoothness all has obvious raising.
Table 4 skin smoothness test chart
| Tested object numbering group | Use front degree of hydration | Use degree of hydration after 14 days |
| 1 | 0.83 | 0.95 |
| 2 | 1.25 | 1.12 |
| 3 | 0.94 | 0.98 |
| 4 | 0.78 | 0.95 |
| 5 | 1.03 | 1.02 |
| 6 | 1.63 | 1.37 |
| 7 | 0.64 | 0.95 |
| 8 | 0.91 | 0.96 |
By universality normal individual trial, test illustrates the antidotal effect of stem cell facial cream passed through of the present invention: from 25-65 year crowd select each 150 of healthy masculinity and femininity, 300 people altogether, generally send the test product of product of the present invention, the feedback capture through trier is as follows:
Have this visible, product of the present invention is tried out on a large scale men and women and is experimentally achieved extraordinary effect, reaches 93.7% to the total effective rate of skin of face.
Claims (7)
1. a grass green salicornia europaeal cell facial cream, is characterized in that:
The grass green salicornia europaeal extract cell facial cream of product of the present invention, comprises following raw material and mixes according to weight ratio:
A group:
B group:
A group is mixed, is heated to 70-75 degree.Deionized water is heated to 70-75 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
2. facial cream according to claim 1, is characterized in that:
Wherein, preferably, method and weight ratio as follows
A group:
B group:
A group is mixed, is heated to 72 degree.Deionized water is heated to 72 degree.A group is added in deionized water, mix homogeneously, stir cooling.
When temperature drops to below 40 degree, add B group, mix homogeneously.
3. facial cream according to claim 1, is characterized in that: described cell extract is stem cell extract.
4. facial cream according to claim 4, is characterized in that: described stem cell extract is placenta stem-cell extract, and its preparation method is:
1), the fragmentation of sheep cord blood stem cell: by sheep cord blood stem cell suspension 50 milliliters, adopt ultrasonic fragmentation, utilize ultrasonic oscillator to launch 9 ~ 10 kilocycle sonicated 3-9 minute;
2), in solution, add 10 times of mL normal saline after, 3000 revs/min, centrifugal 20 minutes;
3), get supernatant, add 20% dehydrated alcohol, mixing, places 15 minutes (ethanol precipitation);
4), by solution 3000 revs/min, centrifugal 20 minutes;
5), abandon supernatant, get precipitation, add normal saline 50 milliliters, shake up gently, resolution of precipitate is become clear solution, be sheep cord blood stem cell activated protein crude extract.
5. facial cream according to claim 1, is characterized in that: described grass green salicornia europaeal method for preparing extractive is:
Be 1 to be mixed with deionized water by grass green salicornia europaeal than the weight ratio of 8-10 according to weight ratio, the temperature remaining on 0-4 DEG C soaks 2 hours to 24 hours, broken grass green salt to suspension, then according to 0.2-0.4 liter/kilogram ratio add chitosan; Adding mass ratio is again 3% ~ 6% ethanol, leaves standstill 2 hours; Get 109AB-8 macroporous resin dress 5 parts, post, under PH6-7, extract concentration gets 50ml upper prop 0.1-0.2 gram often liter time, and loading flow velocity is 2BV/h, first with deionized water clarification, then obtains extract with 3BV50% ethanol according to the flow velocity eluting of 2BV/h.
6. facial cream according to claim 1, is characterized in that: described grass green salicornia europaeal method for preparing extractive is: more preferably, adds the wintergreen oil with weight portions such as grass green salicornia europaeals when soaking grass green salicornia europaeal with deionized water.
7. with the product obtained as method as described in any one in claim 1-6.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105030631A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell face cream and preparation method thereof |
| EP3603613A4 (en) * | 2018-05-24 | 2020-06-17 | Phyto Corporation Inc. | Skin anti-aging composition containing irilin b |
| CN116019737A (en) * | 2023-03-29 | 2023-04-28 | 天齐(内蒙古)生物科技有限公司 | Preparation process and application of easily absorbable bovine and sheep skin collagen peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100362498B1 (en) * | 2000-10-05 | 2002-11-29 | 애경산업(주) | Hamcho containinig cosmetics |
| CN102068391A (en) * | 2010-12-30 | 2011-05-25 | 广州赛莱拉化妆品科技有限公司 | Application of human steam cell growth factors in cosmetics |
| CN102552099A (en) * | 2011-12-30 | 2012-07-11 | 王英丽 | Composite biological agent used for skin beautifying and restoring and preparing method |
-
2014
- 2014-12-11 CN CN201410757310.8A patent/CN105030627A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100362498B1 (en) * | 2000-10-05 | 2002-11-29 | 애경산업(주) | Hamcho containinig cosmetics |
| CN102068391A (en) * | 2010-12-30 | 2011-05-25 | 广州赛莱拉化妆品科技有限公司 | Application of human steam cell growth factors in cosmetics |
| CN102552099A (en) * | 2011-12-30 | 2012-07-11 | 王英丽 | Composite biological agent used for skin beautifying and restoring and preparing method |
Non-Patent Citations (1)
| Title |
|---|
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105030631A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell face cream and preparation method thereof |
| EP3603613A4 (en) * | 2018-05-24 | 2020-06-17 | Phyto Corporation Inc. | Skin anti-aging composition containing irilin b |
| CN116019737A (en) * | 2023-03-29 | 2023-04-28 | 天齐(内蒙古)生物科技有限公司 | Preparation process and application of easily absorbable bovine and sheep skin collagen peptide |
| CN116019737B (en) * | 2023-03-29 | 2023-06-02 | 天齐(内蒙古)生物科技有限公司 | Preparation process and application of easily absorbable bovine and sheep skin collagen peptide |
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