CN112825860A - Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof - Google Patents
Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof Download PDFInfo
- Publication number
- CN112825860A CN112825860A CN202110091420.5A CN202110091420A CN112825860A CN 112825860 A CN112825860 A CN 112825860A CN 202110091420 A CN202110091420 A CN 202110091420A CN 112825860 A CN112825860 A CN 112825860A
- Authority
- CN
- China
- Prior art keywords
- pyraclostrobin
- bactericidal
- regulator
- agent
- alginate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and a preparation method thereof, wherein the bactericidal regulator comprises the following compounds in percentage by mass: 10-50% of alginate-derived oligosaccharide, 10-70% of pyraclostrobin and 10-30% of cosolvent, wherein the cosolvent comprises a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent. The bactericidal regulator prepared by mixing the pyraclostrobin and the alginate-derived oligosaccharide can effectively protect the pyraclostrobin under the condition of not influencing the activity of the medicine, improve the stability of the pyraclostrobin and prolong the lasting period.
Description
Technical Field
The invention relates to the technical field of sterilization regulators, in particular to a sterilization regulator containing alginate oligosaccharides and pyraclostrobin and a preparation method thereof.
Background
Pyraclostrobin (pyraclotrobin), namely chlorothalonil and Pyraclostrobin, is a beta-methoxy acrylate bactericide, has triple effects of protection, treatment and eradication, destroys energy synthesis of pathogenic bacteria by inhibiting respiratory action of the pathogenic bacteria, and is one of agricultural bactericides with the largest usage and the largest sale in the world. Pyraclostrobin has extremely low solubility (1.9g/L) in water, is sensitive to light in aqueous solution, is easy to cause photolysis failure in the preparation and storage processes, and is short in growth period and high in planting density of crops and particularly easy to cause fungal diseases due to the fact that a large amount of out-of-season vegetables and early-maturing fruits are planted at present. Pyraclostrobin is used as a special medicament, and is particularly easy to be used in excess amount and frequently by planters. Thus accelerating the development of disease resistance.
Disclosure of Invention
The invention aims to provide a bactericidal regulator containing brown alginate oligosaccharides and pyraclostrobin and a preparation method thereof.
The above object of the present invention is achieved by the following technical solutions:
a bactericidal regulator containing brown algae oligosaccharide and pyraclostrobin comprises the following compounds in percentage by mass: 10-50% of alginate-derived oligosaccharide, 10-70% of pyraclostrobin and 10-30% of cosolvent, wherein the cosolvent comprises a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent.
The invention is further configured to: the bactericidal regulator can be made into wet dispersant, suspending agent, dust agent, paste, smoke agent, granule, tablet, dry suspending agent, water suspending agent and sustained release agent.
The invention is further configured to: the sterilization regulator consists of the following compounds in percentage by mass: 10% of alginate oligosaccharide, 60% of pyraclostrobin and 30% of cosolvent.
The invention is further configured to: the sterilization regulator consists of the following compounds in percentage by mass: 50% of alginate oligosaccharide, 40% of pyraclostrobin and 10% of cosolvent.
The invention is further configured to: the sterilization regulator consists of the following compounds in percentage by mass: 10% of alginate oligosaccharide, 70% of pyraclostrobin and 20% of cosolvent.
A preparation method of a bactericidal regulator containing brown alginate oligosaccharides and pyraclostrobin comprises the following steps:
(1) preparing a brown algae oligosaccharide solution: cleaning kelp with clear water, pulping into kelp pulp in a pulping machine, adjusting pH to 7-9, adding alginate lyase produced by fermenting strain XS1412C, and stirring for enzymolysis at 25-35 deg.C for 5-13 h; centrifugally separating the kelp residue which is not subjected to enzymolysis, and collecting supernatant to obtain high-purity brown algae oligosaccharide solution;
(2) preparing pyraclostrobin solution: heating and melting pyraclostrobin, and dissolving the pyraclostrobin in a mixed solvent at 0-60 ℃ to prepare a pyraclostrobin solution;
(3) and (3) uniformly mixing the brown algae oligosaccharide solution prepared in the step (1) and the step (2) with the pyraclostrobin solution, then adding a cosolvent, stirring and mixing, and adjusting the pH value to 7-9 to finish the preparation of the sterilization regulator.
In conclusion, the beneficial technical effects of the invention are as follows:
1. pyraclostrobin is a beta-methoxy acrylate bactericide, has triple effects of protection, treatment and eradication, has remarkable functions of regulating immunity, resisting virus, resisting tumor, resisting coagulation, resisting oxidation and the like, can effectively protect pyraclostrobin under the condition of not influencing the activity of a medicament, and can improve the stability and prolong the lasting period by preparing the bactericidal regulator by mixing the pyraclostrobin and the alginate oligosaccharide.
Detailed Description
The present invention will be described in further detail with reference to examples.
In the case of the example 1, the following examples are given,
the invention discloses a bactericidal regulator containing alginate oligosaccharides and pyraclostrobin, which consists of the following compounds in percentage by mass: 10% of brown algae oligosaccharide, 60% of pyraclostrobin and 30% of cosolvent, wherein the cosolvent is composed of a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent.
The bactericidal regulator can be made into wet dispersant, suspending agent, dust agent, paste, smoke agent, granule, tablet, dry suspending agent, water suspending agent and sustained release agent.
A preparation method of a bactericidal regulator containing brown alginate oligosaccharides and pyraclostrobin comprises the following steps:
(1) preparing a brown algae oligosaccharide solution: cleaning kelp with clear water, pulping into kelp pulp in a pulping machine, adjusting pH to 7-9, adding alginate lyase produced by fermenting strain XS1412C, and stirring for enzymolysis at 25-35 deg.C for 5-13 h; centrifugally separating the kelp residue which is not subjected to enzymolysis, and collecting supernatant to obtain high-purity brown algae oligosaccharide solution;
(2) preparing pyraclostrobin solution: heating and melting pyraclostrobin, and dissolving the pyraclostrobin in a mixed solvent at 0-60 ℃ to prepare a pyraclostrobin solution;
(3) and (3) uniformly mixing the brown algae oligosaccharide solution prepared in the step (1) and the step (2) with the pyraclostrobin solution, then adding a cosolvent, stirring and mixing, and adjusting the pH value to 7-9 to finish the preparation of the sterilization regulator.
Example 2
The invention discloses a bactericidal regulator containing alginate oligosaccharides and pyraclostrobin, which consists of the following compounds in percentage by mass: 50% of brown algae oligosaccharide, 40% of pyraclostrobin and 10% of cosolvent, wherein the cosolvent is composed of a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent.
The bactericidal regulator can be made into wet dispersant, suspending agent, dust agent, paste, smoke agent, granule, tablet, dry suspending agent, water suspending agent and sustained release agent.
A preparation method of a bactericidal regulator containing brown alginate oligosaccharides and pyraclostrobin comprises the following steps:
(1) preparing a brown algae oligosaccharide solution: cleaning kelp with clear water, pulping into kelp pulp in a pulping machine, adjusting pH to 7-9, adding alginate lyase produced by fermenting strain XS1412C, and stirring for enzymolysis at 25-35 deg.C for 5-13 h; centrifugally separating the kelp residue which is not subjected to enzymolysis, and collecting supernatant to obtain high-purity brown algae oligosaccharide solution;
(2) preparing pyraclostrobin solution: heating and melting pyraclostrobin, and dissolving the pyraclostrobin in a mixed solvent at 0-60 ℃ to prepare a pyraclostrobin solution;
(3) and (3) uniformly mixing the brown algae oligosaccharide solution prepared in the step (1) and the step (2) with the pyraclostrobin solution, then adding a cosolvent, stirring and mixing, and adjusting the pH value to 7-9 to finish the preparation of the sterilization regulator.
Example 3
The invention discloses a bactericidal regulator containing alginate oligosaccharides and pyraclostrobin, which consists of the following compounds in percentage by mass: 10% of alginate oligosaccharide, 70% of pyraclostrobin and 20% of cosolvent. The cosolvent consists of a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent.
The bactericidal regulator can be made into wet dispersant, suspending agent, dust agent, paste, smoke agent, granule, tablet, dry suspending agent, water suspending agent and sustained release agent.
A preparation method of a bactericidal regulator containing brown alginate oligosaccharides and pyraclostrobin comprises the following steps:
(1) preparing a brown algae oligosaccharide solution: cleaning kelp with clear water, pulping into kelp pulp in a pulping machine, adjusting pH to 7-9, adding alginate lyase produced by fermenting strain XS1412C, and stirring for enzymolysis at 25-35 deg.C for 5-13 h; centrifugally separating the kelp residue which is not subjected to enzymolysis, and collecting supernatant to obtain high-purity brown algae oligosaccharide solution;
(2) preparing pyraclostrobin solution: heating and melting pyraclostrobin, and dissolving the pyraclostrobin in a mixed solvent at 0-60 ℃ to prepare a pyraclostrobin solution;
(3) and (3) uniformly mixing the brown algae oligosaccharide solution prepared in the step (1) and the step (2) with the pyraclostrobin solution, then adding a cosolvent, stirring and mixing, and adjusting the pH value to 7-9 to finish the preparation of the sterilization regulator.
Pyraclostrobin is a beta-methoxy acrylate bactericide, has triple effects of protection, treatment and eradication, has remarkable functions of regulating immunity, resisting virus, resisting tumor, resisting coagulation, resisting oxidation and the like, can effectively protect pyraclostrobin under the condition of not influencing the activity of a medicament, and can improve the stability and prolong the lasting period by preparing the bactericidal regulator by mixing the pyraclostrobin and the alginate oligosaccharide.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.
Claims (6)
1. A bactericidal regulator containing brown algae oligosaccharide and pyraclostrobin is characterized in that: the composition comprises the following compounds in percentage by mass: 10-50% of alginate-derived oligosaccharide, 10-70% of pyraclostrobin and 10-30% of cosolvent, wherein the cosolvent comprises a known dispersing agent, a wetting agent, a defoaming agent, an emulsifying agent, an adhesive and a disintegrating agent.
2. The bactericidal regulator containing alginate oligosaccharides and pyraclostrobin according to claim 1, wherein the bactericidal regulator comprises: the bactericidal regulator can be made into wet dispersant, suspending agent, dust agent, paste, smoke agent, granule, tablet, dry suspending agent, water suspending agent and sustained release agent.
3. The bactericidal regulator containing alginate oligosaccharides and pyraclostrobin according to claim 1, wherein the bactericidal regulator comprises: the sterilization regulator consists of the following compounds in percentage by mass: 10% of alginate oligosaccharide, 60% of pyraclostrobin and 30% of cosolvent.
4. The bactericidal regulator containing alginate oligosaccharides and pyraclostrobin according to claim 1, wherein the bactericidal regulator comprises: the sterilization regulator consists of the following compounds in percentage by mass: 50% of alginate oligosaccharide, 40% of pyraclostrobin and 10% of cosolvent.
5. The bactericidal regulator containing alginate oligosaccharides and pyraclostrobin according to claim 1, wherein the bactericidal regulator comprises: the sterilization regulator consists of the following compounds in percentage by mass: 10% of alginate oligosaccharide, 70% of pyraclostrobin and 20% of cosolvent.
6. The preparation method of the bactericidal regulator containing the alginate oligosaccharides and the pyraclostrobin according to claim 1, which is characterized by comprising the following steps:
(1) preparing a brown algae oligosaccharide solution: cleaning kelp with clear water, pulping into kelp pulp in a pulping machine, adjusting pH to 7-9, adding alginate lyase produced by fermenting strain XS1412C, and stirring for enzymolysis at 25-35 deg.C for 5-13 h; centrifugally separating the kelp residue which is not subjected to enzymolysis, and collecting supernatant to obtain high-purity brown algae oligosaccharide solution;
(2) preparing pyraclostrobin solution: heating and melting pyraclostrobin, and dissolving the pyraclostrobin in a mixed solvent at 0-60 ℃ to prepare a pyraclostrobin solution;
(3) and (3) uniformly mixing the brown algae oligosaccharide solution prepared in the step (1) and the step (2) with the pyraclostrobin solution, then adding a cosolvent, stirring and mixing, and adjusting the pH value to 7-9 to finish the preparation of the sterilization regulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110091420.5A CN112825860A (en) | 2021-01-23 | 2021-01-23 | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110091420.5A CN112825860A (en) | 2021-01-23 | 2021-01-23 | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112825860A true CN112825860A (en) | 2021-05-25 |
Family
ID=75931185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110091420.5A Pending CN112825860A (en) | 2021-01-23 | 2021-01-23 | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112825860A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939977A (en) * | 2012-11-26 | 2013-02-27 | 海南正业中农高科股份有限公司 | Sterilization combination containing oligosaccharide and methoxy acrylic ester ingredient |
| CN103918682A (en) * | 2014-04-01 | 2014-07-16 | 陕西西大华特科技实业有限公司 | Plant immunity bactericidal composition containing chitosan oligosaccharide and pyraclostrobin |
| US20140287919A1 (en) * | 2013-03-14 | 2014-09-25 | Algal Scientific Corporation | Modulation of plant immune system function |
| CN104247710A (en) * | 2013-06-29 | 2014-12-31 | 深圳诺普信农化股份有限公司 | Pyraclostrobin-containing health composition |
| CN104542660A (en) * | 2014-12-03 | 2015-04-29 | 江西省新龙生物科技有限公司 | Bactericidal disease-preventing mixture containing seaweed polysaccharide and pyraclostrobin |
| CN104886093A (en) * | 2015-05-13 | 2015-09-09 | 江西农大植保化工有限公司 | Bactericidal suspending agent containing oligosaccharide and pyraclostrobin |
| CN105613540A (en) * | 2014-11-05 | 2016-06-01 | 江苏龙灯化学有限公司 | Active component composition |
| CN107156156A (en) * | 2017-05-15 | 2017-09-15 | 杨新兰 | A kind of bactericidal composition |
| CN108690857A (en) * | 2018-05-22 | 2018-10-23 | 齐鲁工业大学 | A kind of preparation method of high-purity brown alga oligose |
-
2021
- 2021-01-23 CN CN202110091420.5A patent/CN112825860A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939977A (en) * | 2012-11-26 | 2013-02-27 | 海南正业中农高科股份有限公司 | Sterilization combination containing oligosaccharide and methoxy acrylic ester ingredient |
| US20140287919A1 (en) * | 2013-03-14 | 2014-09-25 | Algal Scientific Corporation | Modulation of plant immune system function |
| CN104247710A (en) * | 2013-06-29 | 2014-12-31 | 深圳诺普信农化股份有限公司 | Pyraclostrobin-containing health composition |
| CN103918682A (en) * | 2014-04-01 | 2014-07-16 | 陕西西大华特科技实业有限公司 | Plant immunity bactericidal composition containing chitosan oligosaccharide and pyraclostrobin |
| CN105613540A (en) * | 2014-11-05 | 2016-06-01 | 江苏龙灯化学有限公司 | Active component composition |
| CN104542660A (en) * | 2014-12-03 | 2015-04-29 | 江西省新龙生物科技有限公司 | Bactericidal disease-preventing mixture containing seaweed polysaccharide and pyraclostrobin |
| CN104886093A (en) * | 2015-05-13 | 2015-09-09 | 江西农大植保化工有限公司 | Bactericidal suspending agent containing oligosaccharide and pyraclostrobin |
| CN107156156A (en) * | 2017-05-15 | 2017-09-15 | 杨新兰 | A kind of bactericidal composition |
| CN108690857A (en) * | 2018-05-22 | 2018-10-23 | 齐鲁工业大学 | A kind of preparation method of high-purity brown alga oligose |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103766353B (en) | Sterilization composition containing fluopyram | |
| CN103283725A (en) | Sterilization composition containing amino-oligosaccharin and validamycin and application of pesticide composition | |
| CN104782670A (en) | Corn seed treatment agent | |
| CN101209045B (en) | Disease prevention suspensible seed coating agent | |
| CN108522546A (en) | A kind of preparation method of sugarcane seed soaking agent | |
| CN112825860A (en) | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof | |
| CN108739897A (en) | A kind of wild jujube presprouting of seeds agent and preparation method thereof | |
| CN105123700B (en) | A kind of insecticidal bactericide containing Thymol and arecaline and preparation method thereof | |
| CN111567532A (en) | Rice high-temperature-resistant yield-increasing conditioner and preparation method and application thereof | |
| CN102204575A (en) | Silktree-albizia-extract-based preparation for controlling tetranychus cinnabarinus, and preparation and application thereof | |
| CN104886139B (en) | Application of the potassium permanganate in corn top-rotten disease is prevented and treated | |
| CN107821163A (en) | A kind of stem of noble dendrobium polyploid special culture media and preparation method thereof | |
| CN113749130A (en) | Sugarcane biological seed stem coating agent and preparation method thereof | |
| CN103858881A (en) | Picoxystrobin and Boscalid-containing sterilization composition | |
| CN109380324A (en) | A kind of blueberry fungicide and preparation method thereof | |
| CN108991044A (en) | A kind of plant source disease-resistant growth-promoting enzyme and preparation method thereof | |
| CN105104389A (en) | Composition containing active ester and chitosan oligosaccharide, and applications thereof | |
| CN109717209A (en) | A kind of novel American Ginseng seed coat agent and preparation method thereof | |
| CN107980799A (en) | A kind of crops downgrade regulating composition | |
| CN109105379A (en) | A kind of preparation and application of chemical bactericide pesticide that preventing and treating blueberry fruit rot | |
| CN105309429A (en) | Pesticidal composition containing choline chloride | |
| CN106982846A (en) | A kind of microbicide compositions containing benzothiostrobin and pyrrole metsulfovax | |
| CN107079915A (en) | Composite bactericide and its application containing fluoxastrobin and jamaicin | |
| CN103843772A (en) | Fungicidal composition containing picoxystrobin | |
| CN108849998A (en) | A kind of composite Nano germinator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210525 |