CN112823212B - 核酸的多重检测 - Google Patents

核酸的多重检测

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Publication number
CN112823212B
CN112823212B CN201980065616.8A CN201980065616A CN112823212B CN 112823212 B CN112823212 B CN 112823212B CN 201980065616 A CN201980065616 A CN 201980065616A CN 112823212 B CN112823212 B CN 112823212B
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China
Prior art keywords
stem
loop
target
oligonucleotide
closed
Prior art date
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CN201980065616.8A
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English (en)
Chinese (zh)
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CN112823212A (zh
Inventor
N·J·哈西克
R·R·金姆
A·L·劳伦斯
A·V·托德
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SpeeDx Pty Ltd
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SpeeDx Pty Ltd
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Priority claimed from AU2018902915A external-priority patent/AU2018902915A0/en
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Publication of CN112823212A publication Critical patent/CN112823212A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/345—DNAzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
CN201980065616.8A 2018-08-09 2019-08-09 核酸的多重检测 Active CN112823212B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2018902915 2018-08-09
AU2018902915A AU2018902915A0 (en) 2018-08-09 Multiplex detection of nucleic acids
PCT/IB2019/056811 WO2020031156A1 (en) 2018-08-09 2019-08-09 Multiplex detection of nucleic acids

Publications (2)

Publication Number Publication Date
CN112823212A CN112823212A (zh) 2021-05-18
CN112823212B true CN112823212B (zh) 2025-09-02

Family

ID=69413249

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980065616.8A Active CN112823212B (zh) 2018-08-09 2019-08-09 核酸的多重检测

Country Status (12)

Country Link
US (1) US12163182B2 (https=)
EP (1) EP3833778A4 (https=)
JP (1) JP7423603B2 (https=)
KR (1) KR102796204B1 (https=)
CN (1) CN112823212B (https=)
AU (1) AU2019316815B2 (https=)
BR (1) BR112021002398A2 (https=)
CA (1) CA3108430A1 (https=)
IL (1) IL280772B2 (https=)
MX (1) MX2021001385A (https=)
SG (1) SG11202101140TA (https=)
WO (1) WO2020031156A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids
AU2020256848A1 (en) * 2020-06-30 2022-10-27 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
CN114941021A (zh) * 2022-05-12 2022-08-26 陕西师范大学 基于探针连接的miRNAs定量检测试剂盒及检测方法
KR20240028084A (ko) 2022-08-24 2024-03-05 주식회사 멀티렉스 표지자가 결합된 태그 프로브 및 이의 용도
CN120019163A (zh) * 2022-09-07 2025-05-16 贝克顿迪金森公司 古细菌聚合酶扩增
EP4627112A1 (en) * 2022-12-01 2025-10-08 Becton, Dickinson and Company Asymmetric hairpin probes for nucleic acid detection
EP4665870A1 (en) * 2023-02-16 2025-12-24 Asecud SA High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2016025452A1 (en) * 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays

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CA2105364A1 (en) * 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
CN1327070A (zh) * 2000-06-03 2001-12-19 庄臣及庄臣研究股份有限公司 以具有催化活性的核酸为基础的诊断方法
EP2385141B1 (en) 2005-10-07 2013-08-07 SpeeDx Pty Ltd Multicomponent nucleic acid enzymes and methods for their use
JP5688507B2 (ja) 2006-10-06 2015-03-25 スピーデクス ピーティーワイ リミテッド 分子スイッチおよびそれらの使用のための方法
MX2009010710A (es) 2007-04-05 2009-12-08 Johnson & Johnson Res Pty Ltd Complejos y enzimas de acido nucleico y metodos para su uso.
CN101241097B (zh) * 2007-09-18 2011-08-03 中国科学院上海应用物理研究所 一种采用茎环结构检测探针的电化学dna检测方法及其试剂盒
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
JP6040227B2 (ja) * 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
CA2863808C (en) * 2012-02-20 2020-07-21 Speedx Pty Ltd Methods and oligonucleotides for amplifying and/or detecting nucleic acids
CN102559918B (zh) * 2012-02-28 2014-10-01 盛司潼 一种利用酶切延伸增加读长的测序方法
JP5958034B2 (ja) * 2012-04-11 2016-07-27 富士レビオ株式会社 分子ビーコン型プローブを用いた標的核酸の検出方法
AR091774A1 (es) * 2012-07-16 2015-02-25 Dow Agrosciences Llc Proceso para el diseño de las secuencias de adn repetidas, largas, divergentes de codones optimizados
EP4697022A2 (en) * 2013-08-09 2026-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US11034997B2 (en) * 2016-09-15 2021-06-15 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids

Patent Citations (1)

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WO2016025452A1 (en) * 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays

Also Published As

Publication number Publication date
AU2019316815B2 (en) 2026-01-08
IL280772A (en) 2021-04-29
NZ772437A (en) 2025-08-29
JP7423603B2 (ja) 2024-01-29
AU2019316815A1 (en) 2021-02-18
MX2021001385A (es) 2021-06-23
WO2020031156A1 (en) 2020-02-13
EP3833778A1 (en) 2021-06-16
SG11202101140TA (en) 2021-03-30
BR112021002398A2 (pt) 2021-05-04
US20220056507A1 (en) 2022-02-24
JP2021533763A (ja) 2021-12-09
US12163182B2 (en) 2024-12-10
CA3108430A1 (en) 2020-02-13
IL280772B2 (en) 2025-04-01
KR20210057028A (ko) 2021-05-20
CN112823212A (zh) 2021-05-18
EP3833778A4 (en) 2022-12-07
IL280772B1 (en) 2024-12-01
KR102796204B1 (ko) 2025-04-14

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