BR112021002398A2 - método para determinar a presença ou ausência de primeiro e segundo alvos em uma amostra e composição - Google Patents
método para determinar a presença ou ausência de primeiro e segundo alvos em uma amostra e composição Download PDFInfo
- Publication number
- BR112021002398A2 BR112021002398A2 BR112021002398-4A BR112021002398A BR112021002398A2 BR 112021002398 A2 BR112021002398 A2 BR 112021002398A2 BR 112021002398 A BR112021002398 A BR 112021002398A BR 112021002398 A2 BR112021002398 A2 BR 112021002398A2
- Authority
- BR
- Brazil
- Prior art keywords
- stem
- loop
- target
- oligonucleotide
- stranded
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/345—DNAzyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2018902915A AU2018902915A0 (en) | 2018-08-09 | Multiplex detection of nucleic acids | |
| AU2018902915 | 2018-08-09 | ||
| PCT/IB2019/056811 WO2020031156A1 (en) | 2018-08-09 | 2019-08-09 | Multiplex detection of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021002398A2 true BR112021002398A2 (pt) | 2021-05-04 |
Family
ID=69413249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021002398-4A BR112021002398A2 (pt) | 2018-08-09 | 2019-08-09 | método para determinar a presença ou ausência de primeiro e segundo alvos em uma amostra e composição |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US12163182B2 (https=) |
| EP (1) | EP3833778A4 (https=) |
| JP (1) | JP7423603B2 (https=) |
| KR (1) | KR102796204B1 (https=) |
| CN (1) | CN112823212B (https=) |
| AU (1) | AU2019316815B2 (https=) |
| BR (1) | BR112021002398A2 (https=) |
| CA (1) | CA3108430A1 (https=) |
| IL (1) | IL280772B2 (https=) |
| MX (1) | MX2021001385A (https=) |
| SG (1) | SG11202101140TA (https=) |
| WO (1) | WO2020031156A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL280772B2 (en) | 2018-08-09 | 2025-04-01 | Speedx Pty Ltd | Multiplex nucleic acid testing |
| MX2022014857A (es) * | 2020-06-30 | 2023-02-23 | Speedx Pty Ltd | Deteccion multiplex de acidos nucleicos mediante el uso de indicadores mixtos. |
| CN114941021A (zh) * | 2022-05-12 | 2022-08-26 | 陕西师范大学 | 基于探针连接的miRNAs定量检测试剂盒及检测方法 |
| KR20240028084A (ko) | 2022-08-24 | 2024-03-05 | 주식회사 멀티렉스 | 표지자가 결합된 태그 프로브 및 이의 용도 |
| CN120019163A (zh) * | 2022-09-07 | 2025-05-16 | 贝克顿迪金森公司 | 古细菌聚合酶扩增 |
| EP4627112A1 (en) * | 2022-12-01 | 2025-10-08 | Becton, Dickinson and Company | Asymmetric hairpin probes for nucleic acid detection |
| WO2024170770A1 (en) * | 2023-02-16 | 2024-08-22 | Asecud Sa | High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2105364A1 (en) * | 1993-09-01 | 1995-03-02 | Eric T. Kool | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| CN1327070A (zh) * | 2000-06-03 | 2001-12-19 | 庄臣及庄臣研究股份有限公司 | 以具有催化活性的核酸为基础的诊断方法 |
| JP5665272B2 (ja) | 2005-10-07 | 2015-02-04 | スピーデクス ピーティーワイ リミテッド | 多成分核酸酵素およびそれらの使用方法 |
| ES2520023T3 (es) | 2006-10-06 | 2014-11-11 | Speedx Pty Ltd | Interruptores moleculares y métodos para su uso |
| US8962238B2 (en) | 2007-04-05 | 2015-02-24 | SpeedDx Pty Ltd | Nucleic acid enzymes and complexes and methods for their use |
| CN101241097B (zh) * | 2007-09-18 | 2011-08-03 | 中国科学院上海应用物理研究所 | 一种采用茎环结构检测探针的电化学dna检测方法及其试剂盒 |
| GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| JP6040227B2 (ja) * | 2011-05-19 | 2016-12-07 | アジーナ バイオサイエンス， インコーポレイテッド | マルチプレックス核酸同定のための製品およびプロセス |
| CA2863808C (en) * | 2012-02-20 | 2020-07-21 | Speedx Pty Ltd | Methods and oligonucleotides for amplifying and/or detecting nucleic acids |
| CN102559918B (zh) * | 2012-02-28 | 2014-10-01 | 盛司潼 | 一种利用酶切延伸增加读长的测序方法 |
| JP5958034B2 (ja) * | 2012-04-11 | 2016-07-27 | 富士レビオ株式会社 | 分子ビーコン型プローブを用いた標的核酸の検出方法 |
| AR091774A1 (es) * | 2012-07-16 | 2015-02-25 | Dow Agrosciences Llc | Proceso para el diseño de las secuencias de adn repetidas, largas, divergentes de codones optimizados |
| CN105555971B (zh) | 2013-08-09 | 2017-08-29 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| EP3180449B1 (en) * | 2014-08-11 | 2019-10-09 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| ES2893411T3 (es) | 2016-09-15 | 2022-02-09 | Hoffmann La Roche | Procedimientos para realizar PCR multiplexada |
| IL280772B2 (en) | 2018-08-09 | 2025-04-01 | Speedx Pty Ltd | Multiplex nucleic acid testing |
-
2019
- 2019-08-09 IL IL280772A patent/IL280772B2/en unknown
- 2019-08-09 SG SG11202101140TA patent/SG11202101140TA/en unknown
- 2019-08-09 CA CA3108430A patent/CA3108430A1/en active Pending
- 2019-08-09 CN CN201980065616.8A patent/CN112823212B/zh active Active
- 2019-08-09 BR BR112021002398-4A patent/BR112021002398A2/pt unknown
- 2019-08-09 JP JP2021507032A patent/JP7423603B2/ja active Active
- 2019-08-09 KR KR1020217006601A patent/KR102796204B1/ko active Active
- 2019-08-09 US US17/266,951 patent/US12163182B2/en active Active
- 2019-08-09 WO PCT/IB2019/056811 patent/WO2020031156A1/en not_active Ceased
- 2019-08-09 EP EP19847536.0A patent/EP3833778A4/en active Pending
- 2019-08-09 MX MX2021001385A patent/MX2021001385A/es unknown
- 2019-08-09 AU AU2019316815A patent/AU2019316815B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112823212B (zh) | 2025-09-02 |
| WO2020031156A1 (en) | 2020-02-13 |
| US12163182B2 (en) | 2024-12-10 |
| EP3833778A4 (en) | 2022-12-07 |
| MX2021001385A (es) | 2021-06-23 |
| JP7423603B2 (ja) | 2024-01-29 |
| IL280772A (en) | 2021-04-29 |
| AU2019316815A1 (en) | 2021-02-18 |
| US20220056507A1 (en) | 2022-02-24 |
| EP3833778A1 (en) | 2021-06-16 |
| IL280772B2 (en) | 2025-04-01 |
| SG11202101140TA (en) | 2021-03-30 |
| NZ772437A (en) | 2025-08-29 |
| IL280772B1 (en) | 2024-12-01 |
| KR102796204B1 (ko) | 2025-04-14 |
| CA3108430A1 (en) | 2020-02-13 |
| KR20210057028A (ko) | 2021-05-20 |
| CN112823212A (zh) | 2021-05-18 |
| JP2021533763A (ja) | 2021-12-09 |
| AU2019316815B2 (en) | 2026-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] |