CN112752763B - 筛查结直肠癌和晚期腺瘤的试剂盒及其应用 - Google Patents

筛查结直肠癌和晚期腺瘤的试剂盒及其应用 Download PDF

Info

Publication number
CN112752763B
CN112752763B CN201980033966.6A CN201980033966A CN112752763B CN 112752763 B CN112752763 B CN 112752763B CN 201980033966 A CN201980033966 A CN 201980033966A CN 112752763 B CN112752763 B CN 112752763B
Authority
CN
China
Prior art keywords
seq
probe
gene
methylation
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980033966.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN112752763A (zh
Inventor
陈一友
李存耀
李慧
郑伟贤
杨蛟
刘刚
吕宁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hangzhou New Horizon Health Technology Co Ltd
Original Assignee
Hangzhou New Horizon Health Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201810502359.7A external-priority patent/CN108660209B/zh
Priority claimed from CN201810502387.9A external-priority patent/CN108676878B/zh
Application filed by Hangzhou New Horizon Health Technology Co Ltd filed Critical Hangzhou New Horizon Health Technology Co Ltd
Publication of CN112752763A publication Critical patent/CN112752763A/zh
Application granted granted Critical
Publication of CN112752763B publication Critical patent/CN112752763B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hospice & Palliative Care (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201980033966.6A 2018-05-23 2019-05-23 筛查结直肠癌和晚期腺瘤的试剂盒及其应用 Active CN112752763B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
CN201810502359.7A CN108660209B (zh) 2018-05-23 2018-05-23 基于bmp3基因甲基化制备结直肠癌早期检测的产品
CN2018105023879 2018-05-23
CN201810502387.9A CN108676878B (zh) 2018-05-23 2018-05-23 检测ndrg4基因甲基化位点的产品在制备结直肠癌早期检测的产品的用途
CN2018105023597 2018-05-23
PCT/US2019/033831 WO2019226942A1 (en) 2018-05-23 2019-05-23 The kit for screening colorectal cancer and advanced adenoma and its application

Publications (2)

Publication Number Publication Date
CN112752763A CN112752763A (zh) 2021-05-04
CN112752763B true CN112752763B (zh) 2025-01-03

Family

ID=68617194

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980033966.6A Active CN112752763B (zh) 2018-05-23 2019-05-23 筛查结直肠癌和晚期腺瘤的试剂盒及其应用

Country Status (10)

Country Link
US (1) US20210207221A1 (https=)
EP (1) EP3797112A4 (https=)
JP (1) JP7637509B2 (https=)
KR (1) KR20210035094A (https=)
CN (1) CN112752763B (https=)
AU (1) AU2019275073A1 (https=)
BR (1) BR112020023274A2 (https=)
CA (1) CA3098028A1 (https=)
SG (1) SG11202010208YA (https=)
WO (1) WO2019226942A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113699237A (zh) * 2020-07-29 2021-11-26 上海吉凯医学检验所有限公司 一种检测早期结直肠癌和腺瘤的dna甲基化标志物的试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399570A (zh) * 2016-11-30 2017-02-15 杭州诺辉健康科技有限公司 用于早期结直肠癌辅助诊断的试剂盒及其使用方法和检测系统

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
EP1942197A1 (de) * 2000-09-01 2008-07-09 Epigenomics AG Verfahren zur Bestimmung des Methylierungsgrades von bestimmten Cytosinen in genomischer DNA im Sequenzkontext 5'-CpG-3'
DE10112515B4 (de) * 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
CN103649298A (zh) * 2011-05-12 2014-03-19 精密科学公司 核酸的分离
WO2013142545A1 (en) * 2012-03-20 2013-09-26 Exact Sciences Corporation Marker panel for detecting cancer
EP4234722A3 (en) * 2014-03-31 2023-09-20 Mayo Foundation for Medical Education and Research Detecting colorectal neoplasm
WO2016094839A2 (en) * 2014-12-12 2016-06-16 Exact Sciences Corporation Compositions and methods for performing methylation detection assays
ES2879964T3 (es) * 2014-12-12 2021-11-23 Exact Sciences Dev Co Llc Composiciones y métodos para realizar ensayos de detección de metilación
CN104975110A (zh) * 2015-06-02 2015-10-14 浙江诺辉生物技术有限公司 用于检测生物样本中bmp3和ndrg4甲基化水平的引物和探针
AU2016343937B2 (en) * 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
WO2018087129A1 (en) * 2016-11-08 2018-05-17 Region Nordjylland, Aalborg University Hospital Colorectal cancer methylation markers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399570A (zh) * 2016-11-30 2017-02-15 杭州诺辉健康科技有限公司 用于早期结直肠癌辅助诊断的试剂盒及其使用方法和检测系统

Also Published As

Publication number Publication date
WO2019226942A1 (en) 2019-11-28
EP3797112A1 (en) 2021-03-31
US20210207221A1 (en) 2021-07-08
KR20210035094A (ko) 2021-03-31
AU2019275073A8 (en) 2020-12-10
JP2021526370A (ja) 2021-10-07
BR112020023274A2 (pt) 2021-02-23
SG11202010208YA (en) 2020-11-27
CA3098028A1 (en) 2019-11-28
EP3797112A4 (en) 2022-03-23
JP7637509B2 (ja) 2025-02-28
CN112752763A (zh) 2021-05-04
AU2019275073A1 (en) 2020-11-26

Similar Documents

Publication Publication Date Title
US11021758B2 (en) Methods for detecting gene dysregulation by intragenic differential expression
CN115354076A (zh) 通过使用平均循环阈值的基因内差异表达(ide)检测基因融合
CN113215264B (zh) 用于子宫内膜癌早期筛查人外周血循环肿瘤dna中tmem101基因甲基化检测试剂盒
EP2798088B1 (en) Detection of adenomas of the colon or rectum
US12584165B2 (en) Method for identifying one or more mutations in a hotspot mutation sequence
US20220267859A1 (en) Use of detection reagent for detecting methylation of genes associated with colorectal cancer, and kit
AU2012388616A1 (en) Method for screening cancer
CN112752763B (zh) 筛查结直肠癌和晚期腺瘤的试剂盒及其应用
CN115094139B (zh) 检测甲基化水平的试剂在制备膀胱癌诊断产品中的应用以及膀胱癌诊断试剂盒
RU2812023C2 (ru) Набор для скрининга на рак прямой и ободочной кишки и прогрессирующей аденомы и его применение
HK40042105A (en) The kit for screening colorectal cancer and advanced adenoma and its application
HK40042105B (zh) 筛查结直肠癌和晚期腺瘤的试剂盒及其应用
JP7821511B2 (ja) ＣｐＧ領域メチル化レベルを利用した大腸癌診断用組成物及びその用途
CN120272593A (zh) 用于诊断胃癌的甲基化生物标记物及其应用
CN109207597A (zh) 一种用于检测头颈癌变异程度的检测套组
BR112016021878B1 (pt) Métodos para detectar uma desregulação em um gene alvo e para diagnosticar a presença ou ausência de câncer ou uma susceptibilidade ao câncer em um indivíduo

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40042105

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant