CN112741086B - Application of marigold volatile beta-cyclohomocitral to control of medlar aphids - Google Patents

Application of marigold volatile beta-cyclohomocitral to control of medlar aphids Download PDF

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CN112741086B
CN112741086B CN202110163736.0A CN202110163736A CN112741086B CN 112741086 B CN112741086 B CN 112741086B CN 202110163736 A CN202110163736 A CN 202110163736A CN 112741086 B CN112741086 B CN 112741086B
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marigold
medlar
aphids
aphid
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CN112741086A (en
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刘畅
王芳
何嘉
张蓉
魏淑花
陈宏灏
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Institute of Plant Protection of Ningxia Academy of Agriculture and Forestry Sicience
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/20—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings

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Abstract

The invention discloses application of marigold volatile matter beta-cyclohomocitral to control medlar aphids, wherein the marigold volatile matter component is beta-cyclohomocitral which is collected from flowers, stems and leaves of marigold or the whole plant of marigold. The invention also provides application of the aphid prevention and control composition containing marigold volatile matters in preventing and controlling medlar aphids. The invention provides test data support for effectively preventing and controlling medlar aphid harm, developing a new-generation efficient and environment-friendly botanical pesticide, and simultaneously provides scientific basis for further developing and utilizing marigold.

Description

Application of marigold volatile beta-cyclohomocitral to control of medlar aphids
Technical Field
The invention belongs to the technical field of agriculture, relates to an ecological regulation and control technology of medlar aphids, and particularly relates to screening of aversion plants capable of preventing and controlling medlar aphids, and application of prevention and control of medlar aphids by adopting volatile matters of the aversion plants.
Background
Aphids are important agricultural pests, damaging field and greenhouse crops of more than 350 species in as many as 50 families, and transmitting more than 100 plant viruses. The Chinese wolfberry aphid (Aphis sp.) is commonly called nectar, greasy insect, drought insect, etc., and belongs to homoptera and aphididae agricultural pests. The aphids of the medlar harm young fruits of medlar leaf flower devices, so that withered flowers and fruits on branch tips are reduced, and the yield and the quality of the medlar are seriously influenced. The aphid of the Chinese wolfberry overwinter on the branch by eggs, damage is started after the Chinese wolfberry sprouts in 3-4 months, and the 1 st peak appears in 6 months; the insect population in the summer is reduced to some extent, and then rises again after autumn, and the peak of the 2 nd time appears in the 9 th month. A large number of adult aphids gather young tips, basal parts of buds and leaves of the medlar and prick and suck juice on the backs of the buds and the leaves, which seriously influences the blossoming, fruiting, growth and development of the medlar and is one of three main pests for planting the medlar. The prevention and control of the aphids of the medlar have important significance for improving the yield and the quality of the medlar.
The long-term use of chemical pesticides in large quantities brings various disadvantages, such as the generation of drug resistance of pests, residual toxicity and environmental pollution, and the harmful effects of killing a large number of natural enemies of the pests and seriously destroying the ecological balance. How to improve the effective utilization rate of pesticides and reduce the dosage of pesticides in non-target environments becomes an urgent problem to be solved in the pesticide subject of China, and researches on the reasonable pesticide use technology, especially one of the hot spots for gradually developing the current agricultural and pharmaceutical research by application exploration of botanical pesticides. In recent years, the main direction of research on plant-derived pesticides has focused on finding insecticidal and bactericidal active ingredients from plant secondary metabolites.
The medlar is the first of five treasures in Ningxia, and is one of the dominant agricultural advantages industries in Ningxia, and the medlar planting area in the whole area exceeds 90 ten thousand mu and occupies more than half of the planting area in China at present. With the increasing of planting area and export amount of Ningxia wolfberry, Ningxia wolfberry product quality is concerned at home and abroad more and more, especially, wolfberry product export is returned due to the influence of quality safety such as pesticide residue exceeding standard and the like and factors such as export 'green barrier', pesticide residue becomes a main obstacle for the safety and export of Ningxia wolfberry products, so that the quality safety of wolfberry is enhanced, and the method is more suitable for promoting ecological regulation and control technology to prevent and control pests.
Disclosure of Invention
In order to fundamentally eliminate the problem of pesticide residue of medlar products and achieve effective prevention and control of medlar aphids, an ecological regulation and control technology is implemented in a medlar plantation to prevent and control pests, and the method is more in line with the concepts of environmental protection, health and sustainable development. Aiming at the problems of aphid harm and medlar quality safety to be improved commonly existing in medlar planting, the invention seeks the influence of marigold volatile matters on medlar aphids based on the research of screening early medlar aphid avoiding plants, and provides an aphid prevention and control composition containing marigold volatile matters on the basis of the influence of the marigold volatile matters on medlar aphids, so that medlar aphid harm can be effectively prevented and controlled, test data support is provided for the development of a new generation of plant source pesticides with high efficiency and environmental protection, and a feasible aphid prevention and control scheme is provided for organic medlar planting.
Specifically, the invention provides an aphid prevention and control composition containing marigold volatile matters, which comprises marigold volatile matter components having an aphid repelling effect, wherein the marigold volatile matter components are any one of p-alpha-dimethyl styrol, beta-cyclohomocitral, verbenol and caryophyllin. The prior patent application CN201710974117.3 discloses a method for preventing and controlling medlar aphid by introducing an aversion plant into a medlar garden, wherein marigold has the strongest aversion to medlar aphid and is more suitable for the soil quality of medlar gardens in Ningxia regions. The application of the patent analyzes and researches the chemical components of volatile matters at different parts of marigold, and the volatile matters of marigold are identified to contain p-alpha-dimethyl styrax, beta-cyclohomocitral, verbenol and caryophyllin, and the specific chemical structures of the volatile matters are shown in table 1.
TABLE 1 chemical Structure of volatile component of marigold
Figure BDA0002936647700000021
Figure BDA0002936647700000031
According to the result of the determination of an antennal potentiostat, volatile matters in different parts (flowers, stems, leaves and the whole plant) of marigold have certain influence on the antennal potentiality of the medlar aphid, and the difference is not obvious. Therefore, as a preferred embodiment of the technical scheme of the invention, the marigold volatile matters are collected from flowers, stems and leaves of marigold or whole plants of marigold.
Furthermore, the volatile component of marigold is verbenol. The invention discusses the sexual behavior reaction of different verbenol concentrations to the medlar aphid, and obtains that the using concentration of the verbenol avoiding the medlar aphid is not lower than 0.1 mu g/mu L.
Regarding the dosage form of the aphid control composition containing marigold volatile matters, the aphid control composition can be prepared into any dosage form used in agriculture, such as microcapsule sustained release agent, aerosol, (ultra) low volume preparation and the like. Further preferably, the present invention recommends that the dosage form of the aphid control composition containing marigold volatile matter is microcapsule sustained-release preparation, and the preparation method is related to the concrete preparation method, and those skilled in the art can make comprehensive reference to the methods disclosed in the prior patents CN101176726A and CN101779626B, which are not described in detail in the present application.
On the other hand, the invention also discloses application of the aphid prevention and control composition containing marigold volatile matters in preventing and controlling medlar aphids.
Compared with the prior art, the aphid prevention and control composition containing marigold volatile matter has at least the following beneficial effects or advantages: according to the research of the inventor group, the marigold planted in the medlar plantation has a certain control effect on the occurrence of medlar pests. Based on the research result, the invention further proves that marigold volatile matter has a repellent effect on the medlar aphid through the tropism behavior reaction of the medlar aphid on the fragrance plant odor. According to the invention, marigold volatile matters are collected, and GC-MS and GC-EAD technologies are utilized to identify that the components in the marigold volatile matters at least comprise p-alpha-dimethyl styrol, beta-cyclohomocitral, verbenol and caryophyllin. Wherein, the verbenol has different degrees of influence on escape behavior and olfactory behavior of the Chinese wolfberry aphid, formation of winged aphid of the Chinese wolfberry aphid and population individual quantity. The invention provides test data support for effectively preventing and controlling medlar aphid harm, developing a new-generation efficient and environment-friendly botanical pesticide, and simultaneously provides scientific basis for further developing and utilizing marigold.
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FIG. 1 is a schematic view of a device for collecting marigold volatiles according to an embodiment.
FIG. 2 is a GC-EAD plot of marigold volatile components versus α -dimethyl styrol as described in the examples.
FIG. 3 is a mass spectrum of marigold volatile components versus α -dimethyl styrol as described in the examples.
FIG. 4 is a GC-EAD graph of beta-cyclohomocitral of the volatile component of marigold as described in the examples.
FIG. 5 is a mass spectrum of beta-cyclohomocitral as a volatile component of marigold according to the examples.
FIG. 6 is a GC-EAD plot of verbenol, a volatile component of marigold as described in the examples.
FIG. 7 is a mass spectrum of verbenol which is a volatile component of marigold according to the examples.
FIG. 8 is a GC-EAD plot of the volatile component dianilin from marigold as described in the examples.
FIG. 9 is a mass spectrum of the volatile component dianthus chinensis from marigold described in the examples.
FIG. 10 is a graph showing the effect of verbenol on aphid escape behavior in Lycium barbarum as described in the examples.
FIG. 11 is a graph showing the results of the olfactory behaviors of verbenol on aphids of Lycium barbarum as described in the examples.
FIG. 12 is a graph showing the effect of verbenol on the formation of Aphis ptera as described in the examples.
FIG. 13 is a graph of the results of the individual number effects of verbenol on aphid populations of Lycium barbarum as described in the examples.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1 collection and determination of volatile matter from marigold plants
The insect to be tested is the aphid (Aphis sp.) of Lycium barbarum raised indoors, the aphid of Lycium barbarum collected from Ningxia Zhongning Lycium barbarum is inoculated on Lycium barbarum planted in greenhouse, the inoculated aphid is removed after two days, and wingless adult aphid with the same size and insect state as the test insect is selected
Collecting marigold flower, stem leaf and whole plant volatile substance samples by using a dynamic headspace adsorption method. The collection device for marigold volatile substances is shown in figure 1. The Parpark adsorbent was packed into an adsorption column, eluted with 0.5ml dichloromethane (chromatographically pure), rinsed three times, then concentrated with high purity nitrogen and a sample of the volatiles collected to around 0.2 ml. And analyzing the activity of the volatile matters of the concentrated sample by using a gas chromatography antennal potential combination instrument (GC-EAD), and carrying out detailed comparison according to the peak shape and retention time of the compound on a GC-EAD chromatogram so as to identify the active substance. The response values of different parts (flower, stem and leaf and whole plant) of marigold by an antennal potentiostat are shown in Table 2.
TABLE 2 Tagetes erecta different parts electroantennometer reaction values
Plant parts Reaction value (mv)
Flower of marigold 0.1069±0.0297a
Caulis et folium Tagetis Erectae 0.1065±0.0271a
Whole plant marigold 0.0481±0.0226a
As can be seen from the table 1, volatile matters in different parts of marigold have certain influence on the antennal potential of the aphid of the medlar, but the influence difference is not obvious. Therefore, marigold volatile samples for subsequent experiments were collected from the whole plant.
Volatile matter component analysis (GC-MS) of the whole marigold plant: sampling volatile substance samples of whole plant of flos Tagetis Erectae, sampling 2ul, sampling mode is no split flow sampling, detector temperature is 260 deg.C, column temperature adopts programmed temperature raising method, 50 deg.C is maintained for 1 min, raising to 120 deg.C at 5 deg.C/min is maintained for 0min, raising to 240 deg.C at 10 deg.C/min is maintained for 10min, and total operation time is 35 min.
The results of the volatile content measurement of marigold plants are shown in FIGS. 2-9. Specifically, it is assumed from the combination of FIGS. 2 and 3 that the whole plant volatile of marigold contains p- α -dimethylstyrol. By combining FIG. 4 and FIG. 5, it is assumed that the whole plant volatile matter of marigold contains beta-cyclohomocitral. It is assumed from FIG. 6 and FIG. 7 that the whole plant volatile of marigold contains verbenol. It is assumed from the combination of FIGS. 8 and 9 that the whole plant volatile matter of marigold contains caryophyllin.
Example 2 Studies on repellent action of verbenol on aphids of Chinese wolfberry
Based on the embodiment 1, the repellent effect of verbenol on medlar aphids is mainly researched, and the specific research contents comprise the influence of the verbenol with different concentrations on escape behaviors of the medlar aphids, the olfactory behavior reaction of the medlar aphids, the formation of winged aphids on the medlar aphids and the influence of population individual quantity.
Data from the experiment are presented as mean ± sem. Analyzing the data of the influence of the verbenol with different concentrations on escape behaviors of the Chinese wolfberry aphids, the formation of the winged aphids and the population individual quantity by using One-way ANOVA of DPS (data processing System); the olfaction behavior reaction data of verbenol with different concentrations on the medlar aphid in a long distance are subjected to difference significance by chi-square test.
(1) Effect of verbenol of different concentrations on escape behavior of medlar aphid
A transparent ecological box (2.5cm multiplied by 2.5cm) is divided into two areas to observe the escape number of the medlar aphids, so that the problem that the areas of a single culture dish or a mesh enclosure method are not clearly divided is solved. Lightly clamping the leaves of the medlar inhabiting aphids in an ecological box, respectively dripping 10 mu L of active ingredient solutions with different concentrations on filter paper with the thickness of 1cm multiplied by 1cm, supporting the active ingredient solutions by toothpicks and placing the active ingredient solutions at the bottom of the ecological box, and connecting the leaves of the medlar living body clamped by the ecological box with 3-year-old aphids with the sizes of 20 head worms basically consistent; counting the number of aphids escaping from the leaf to the other end of the ecological box within 3 min; each concentration was repeated 3 times. The experimental temperature is 20 +/-1 ℃ and the relative humidity is 60 +/-5%.
The influence result of verbenol on escape behavior of Chinese wolfberry aphid is shown in figure 10. As can be seen from FIG. 10, in the concentration range of 0-10. mu.g/. mu.L, analyzed, the escape behavior of the medlar aphid is not significant when the concentration of verbenol is 0.001. mu.g/. mu.L and 0.01. mu.g/. mu.L; when the concentration of the verbena enol is 0.1 mu g/mu L, the escape behavior of the medlar aphids is obvious, and the escape number of the medlar aphids is increased along with the increase of the concentration of the verbena enol.
(2) Olfactory action reaction of medlar aphid to verbenol with different concentrations
And measuring olfactory action reaction of the medlar aphids to verbenol with different concentrations by adopting a Y-type olfactometer. The Y-type olfactometer is designed and manufactured according to Du and the like, the Y-type olfactometer is composed of colorless transparent glass tubes with 3 arms, the length of each arm is 10cm, the inner diameter of each arm is 2cm, the included angle between the two arms is 60 degrees, the atmosphere collection instrument is used as an air pump, and air sequentially enters the two arms through an activated carbon odor source bottle and a flowmeter. During the experiment, the indoor temperature is 20 +/-1 ℃, the air flow rate is 100mL/min, the relative humidity is 60 +/-5%, and 2 12W fluorescent lamps are placed at the positions 30cm above the two arms, so that the light of the two arms of the Y-shaped tube is uniform, and the influence on the behavior reaction of aphids is reduced.
Respectively placing filter paper containing verbena enol solution and control (not containing the verbena enol solution) in a taste source bottle, sequentially carrying out experiments from low concentration to high concentration, releasing aphids at the position l/2 of the base of a glass tube, recording the reaction of the aphids within 5min, and regarding the aphids as reaction when the aphids reach one arm and exceed 5cm, otherwise, regarding the aphids as non-reaction. Each test was performed once, and two arms were exchanged for each 5 th test, and the Y-glass tube was wiped with absolute ethanol. 3 groups of 20-22 replicates per concentration were tested for a total of 60 < 3 > Aphis. After each concentration test is finished, all the glass tubes and the connecting rubber tubes are respectively cleaned by absolute ethyl alcohol and distilled water.
Olfactory behavioral responses of aphids of lycium barbarum to different concentrations of verbenol are shown in fig. 11. As can be seen from figure 11, the concentration of verbena enol is 0.001 mu g/mu L and 0.01 mu g/mu L, which has attraction effect but is not significant to the medlar aphid, the concentration of verbena enol reaches 0.1 mu g/mu L, which has significant repellent effect, and the repellent quantity of verbena enol increases with the increase of the concentration.
(3) Effect of different concentrations of verbenol on the formation of winged aphids and the population number of the Lycium barbarum aphids
Referring to Kunert et al, 15 1 st aphids were inoculated onto seedlings of Lycium barbarum, 10. mu.L of verbena enol solution was dropped onto filter paper, fixed with toothpicks in the center of a basin of Lycium barbarum, dropped 5 times per day (the dropping time was set at 9:00, 11:30, 14:00, 16:30 and 19:00), treated for 5 days continuously, the number of deaths of aphids was observed and recorded every day, the wing shape was recorded after development to aphid formation, and the total number of aphids was recorded after 2 weeks. Each concentration was repeated 5 times.
The influence of the verbenol with different concentrations on the formation of the winged aphids is shown in figure 12, and as can be seen from figure 12, the influence of the verbenol with different concentrations on the formation of the winged aphids is not significant, when the concentration of the verbenol reaches 10 mug/muL, the death rate of the aphids is too high, and the formation number of the winged aphids is obviously reduced.
The influence of the verbenol with different concentrations on the individual quantity of the aphid population of the medlar is shown in figure 13, and as can be seen from figure 13, in the concentration range of 0-10 mug/muL, the verbenol has no significant influence on the individual quantity of the aphid population of the medlar when the concentration of the verbenol is 0.001 mug/muL and 0.01 mug/muL through analysis; when the concentration of verbena enol reaches 0.1 mug/muL, the number of individual aphid populations can be obviously inhibited, and the inhibition effect is increased along with the increase of the concentration.
Example 3 preparation of microcapsule sustained-release formulation containing marigold volatile substance and evaluation of control effect on aphid of Lycium barbarum
(1) Preparation of microcapsule slow-release agent
Beta-cyclodextrin is respectively used for clathrating marigold volatile substance p-alpha-dimethyl storax, beta-cyclohomocitral, verbenol and caryophyllin to prepare the marigold volatile substance beta-cyclodextrin clathrate. The preparation process of the inclusion compound is described as follows:
accurately weighing beta-cyclodextrin, placing the beta-cyclodextrin in a glass beaker, adding a certain amount of distilled water, and preparing into a saturated solution; fully mixing and dissolving marigold volatile matters in 3-5 times of anhydrous ethanol, opening a stirrer when the temperature of the saturated solution is up to a fixed temperature, slowly dripping the prepared marigold volatile matter solution while stirring, keeping continuously stirring after dripping is finished, and taking out; cooling the inclusion beta-cyclodextrin solution to room temperature, refrigerating the inclusion beta-cyclodextrin solution in a refrigerator at 4 ℃, extracting under reduced pressure after 24h, and washing with absolute ethyl alcohol for 3 times in the extraction process until marigold volatile matters which are not included on the surface of the inclusion compound are washed. Placing the extracted inclusion compound in a drying oven with constant temperature of 50 ℃ for 4h to obtain the marigold volatile substance beta-cyclodextrin inclusion compound.
The prepared marigold volatile substance beta-cyclodextrin inclusion compound is used as an effective component for preventing and controlling medlar aphids, and is prepared into 3 percent of p-alpha-dimethyl styrol microcapsule slow release agent, 3 percent of beta-cyclohomocitral microcapsule slow release agent, 3 percent of verbenol microcapsule slow release agent and 3 percent of caryophyllin microcapsule slow release agent according to the prior art.
(2) Evaluation of control effect of aphids of medlar
The 3% p-alpha-dimethyl styrol microcapsule slow release agent, the 3% beta-cyclohomocitral microcapsule slow release agent, the 3% verbenol microcapsule slow release agent and the 3% caryophyllin microcapsule slow release agent are used as test agents, and the pyrethrin is used as a contrast agent.
The number of insect population is investigated before drug application in 7-month and 25-day of 2018, the number of residual insects is investigated 1 day after drug application in 26-month and 7-month, the number of residual insects is investigated 3 days after drug application in 28-month and 7-month, the number of residual insects is investigated 7 days after drug application in 1-month and 8-month, the number of residual insects is investigated 14 days after drug application in 8-month and 8-day, and the number of residual insects is investigated 21 days after drug application in 15-month and 8-day, wherein the number of residual insects is investigated for 6 times. According to an investigation method of local standard DB64/T852-2013, randomly drawing 5 branches per cell, and investigating and recording the number of insect mouths of the Chinese wolfberry aphids within the range of 30cm of the top tip of each branch.
The test investigation result calculates the prevention and treatment effect according to the following formula, and the result is subjected to statistical analysis and significance determination by adopting a new level difference method on DPS statistical software. The statistical results of the control effect of the medicament on the medlar aphid are shown in table 3.
Calculating the formula:
Figure BDA0002936647700000081
in the formula: PT0-number of predrug insects in the agent treatment area; PT1-post-drug worm number in the drug treatment area; CK (CK)0-number of predose insects in the placebo zone; CK (CK)1Number of insects after drug administration in the placebo zone.
TABLE 3 control Effect of aphid control compositions containing marigold volatiles on medlar aphid
Figure BDA0002936647700000082
The results in table 3 show that 3% of p-alpha-dimethyl styrol microcapsule sustained release agent, 3% of beta-cyclohomocitral microcapsule sustained release agent, 3% of verbenol microcapsule sustained release agent, 3% of caryophyllin microcapsule sustained release agent and pyrethrin all have certain control effect on medlar aphids and can be used for controlling medlar aphids. 3 days after the pesticide is applied, the control effect of the pyrethrin on medlar aphid is better, and the control effect is better than that of marigold volatile matters. In the marigold volatile substance microcapsule sustained release preparation, the control effect of the verbenol microcapsule sustained release preparation is equivalent to that of pyrethrin 1 day after the application, and is obviously superior to other marigold volatile substances; 14 days after the drug is applied, the control effect of the verbenol microcapsule sustained release agent can still reach 65.02 percent; after 21 days, the control effect of the verbenol microcapsule sustained-release preparation is equivalent to that of p-alpha-dimethyl styrol, and the control effect is superior to other marigold volatile matters and pyrethrin.
The present invention has been further described with reference to the examples, but the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims (1)

1. The application of beta-cyclohomocitral which is a volatile substance of marigold in preventing and treating medlar aphids is characterized in that the beta-cyclohomocitral is collected from flowers, stems and leaves of marigold or the whole plant of marigold and has influence on the antenna potential of medlar aphids;
the beta-cyclodextrin microcapsule slow release agent of 3 percent beta-cyclohomocitral has the administration dose of 300g a.i.·hm-2。
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