CN109819982A - The aphid prevention and control composition of the volatile matter containing marigold and its application - Google Patents
The aphid prevention and control composition of the volatile matter containing marigold and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/20—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings
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Abstract
The present invention discloses a kind of aphid prevention and control composition of volatile matter containing marigold, the composition includes the marigold volatile component for having avoidance effect to aphid, and the marigold volatile component is any one of p- alpha-alpha-dimethyl styrene, β-homocyclocitral, verbenol, caryophyllin.The marigold volatile matter is collected in the whole strain plant of flower, cauline leaf or marigold of marigold.The present invention gives application of the aphid prevention and control composition of the volatile matter containing marigold on prevention and treatment Wolfberry Aphid.The present invention is the harm of effective prevention and control Wolfberry Aphid, provides test data support for botanical pesticide of new generation exploitation efficiently, environmentally friendly, while providing scientific basis for further development and utilization marigold.
Description
Technical field
The invention belongs to agricultural technology fields, are related to the ecological regulation and control technology of Wolfberry Aphid, and in particular to can prevention and control fructus lycii
The screening for walking quickly and keeping away plant of aphid, and the application using the volatile matter prevention and control Wolfberry Aphid for walking quickly and keeping away plant.
Background technique
Aphid is important agricultural pests, more than the crop field caused harm and up to more than 50 sections 350 of chamber crop, and can pass
Broadcast 100 various plants virus.Wolfberry Aphid (Aphis sp.) is commonly called as aphid, aphid, non-irrigated worm etc., belongs to Homoptera, the agriculture of aphid section
Industry pest.Wolfberry Aphid endangers wolfberry leaf piece floral organ young fruit, so that branch atrophy flowers and fruits is become smaller, seriously affects yield of medlar and matter
Amount.Wolfberry Aphid is overwintering on branch with ovum, since 3-4 month fructus lycii germination after endanger, until there is the 1st peak June；
Declined into midsummer insect population, enters after autumn multiple rising again, until the 2nd peak occurs in September part.Largely at, larvae aphid cluster fructus lycii
Tender tip, bud-leaf base portion and blade back piercing and sucking juice seriously affect fructus lycii and yield positive results and growth and development, are that the three of fructus lycii plant are big main
Want one of pest.Prevention and treatment Wolfberry Aphid has great importance to the yield and quality raising of fructus lycii.
Long-term a large amount of uses of chemical pesticide bring various drawbacks, the generation of such as harmful biomolecule resistant, residual hazard and
Environmental pollution kills a large amount of pest natural enemies, seriously destroys the ecological balance in which cannot tell the good from the bad.How pesticide effective use is improved
Rate reduces injected volume of the pesticide in non-target environment, it has also become China's pesticide subject urgent problem to be solved, pesticide rationally make
Gradually develop one of the hot spot of current Pesticide Science research with the explorative research of the research of technology, especially botanical pesticide.In recent years
Come, the Main way of botanical pesticide research concentrates on finding desinsection, fungicide active ingredient from Plant Secondary Metabolites.
Fructus lycii is one of the agriculture advantage leading industry in Ningxia, whole district fructus lycii is planted at present as first of Ningxia " five precious "
Area is more than 900,000 mu, Zhan Quanguo cultivated area more than half.As lycium barbarum cultivated area, export volume are continuously increased,
Lycium barbarum product quality is increasingly by concern both domestic and external, especially fructus lycii products export often due to the residual matter such as exceeded of agriculture
Amount is safe and exports the influences of factors such as " green barriers " and is returned goods, and pesticide residue has become lycium barbarum product safety and goes out
Therefore the major obstacle of mouth reinforces Lycium Quality Safety, be more applicable for carrying out the progress control of insect of ecological regulation and control technology.
Summary of the invention
In order to be inherently eliminated the Pesticide Residue of fructus lycii product, while reaching effective prevention and control of Wolfberry Aphid,
Fructus lycii, which is planted, carries out the progress control of insect of ecological regulation and control technology in garden, more meet environmental protection, health, the theory of sustainable development.This
Invention is for generally existing aphid damage in fructus lycii plantation and has Lycium Quality Safety problem to be hoisted, is based on Chinese holly early period
Qi aphid walks quickly and keeps away the research of foliage filter screening, has sought influence of the marigold volatile matter to Wolfberry Aphid, and based on this, provides
A kind of aphid prevention and control composition of the volatile matter containing marigold can effectively endanger prevention and control Wolfberry Aphid, for new one efficiently, environmentally friendly
It is developed for botanical pesticide and test data support is provided, provide the anti-prosecutor of practicable aphid for organic plantation of fructus lycii
Case.
Specifically, the present invention provides a kind of aphid prevention and control composition of volatile matter containing marigold, and the composition includes pair
Aphid have avoidance effect marigold volatile component, the marigold volatile component be p- alpha-alpha-dimethyl styrene,
Any one of β-homocyclocitral, verbenol, caryophyllin.Earlier patent application CN201710974117.3 discloses one kind
Using the method for walking quickly and keeping away plant prevention and control Wolfberry Aphid is introduced in wolfberry orchard, wherein marigold to the phobotaxis of Wolfberry Aphid most
By force, and relatively it is suitble to the soil property in Ningxia, China fructus lycii plantation.Present patent application to marigold different parts volatile matter chemistry at
Analyze and research, it is identified, in marigold volatile component containing p- alpha-alpha-dimethyl styrene, β-homocyclocitral,
Verbenol, caryophyllin, particular chemical are shown in Table 1.
The chemical structure of 1 marigold volatile component of table
It is obtained according to the measurement result of tentaculum electric potential instrument, marigold different parts (flower, cauline leaf, whole strain) volatile matter is to fructus lycii
The tentaculum electric potential of aphid all has certain influence, and difference is not significant.Therefore, the preferred implementation side as technical solution of the present invention
Formula, the marigold volatile matter are collected in the whole strain plant of flower, cauline leaf or marigold of marigold.
Further, the marigold volatile component selects verbenol.The present invention has inquired into different verbenenes
Determining alcohol show that the verbenol is walked quickly and kept away the use concentration of Wolfberry Aphid and is not less than to Wolfberry Aphid taxis behavior reaction
0.1μg/μL。
The dosage form of aphid prevention and control composition about the volatile matter containing marigold can be prepared into times agriculturally used
A kind of dosage form, such as controlled release formulation of capsule, aerosol, (super) low capacity preparation etc..It is further preferred that described in present invention recommendation
The dosage form of the aphid prevention and control composition of the volatile matter containing marigold is controlled release formulation of capsule, the specific preparation method in relation to preparation, sheet
Field those of ordinary skill can integrate the method system referring to disclosed in existing patent CN101176726A, CN101779626B
Standby, the present patent application is not described in detail this.
On the other hand, the present patent application also discloses the aphid prevention and control composition of the volatile matter containing marigold in prevention and treatment Chinese holly
Application on Qi aphid.
Compared with prior art, the aphid prevention and control composition of the volatile matter of the present invention containing marigold at least has following
Beneficial effect or advantage: the research of people team obtains according to the present invention, makees marigold between fructus lycii plantation to Lycium Pests
Generation have certain control action.Based on this research achievement, the present invention is by Wolfberry Aphid to fragrant plant smell
Taxis behavior reaction, further demonstrating marigold volatile matter has repellent action to Wolfberry Aphid.The present invention is by collecting ten thousand
Longevity chrysanthemum volatile matter, using GC-MS and GC-EAD technology, the component identified in marigold volatile matter includes at least p- alpha, alpha-dimethyl
Base styrene, β-homocyclocitral, verbenol, caryophyllin.Wherein, verbenol to Wolfberry Aphid flight behavior,
Olfactory behavior, forms Wolfberry Aphid alatae and population at individual quantity has different degrees of influence.The present invention is effectively anti-
Control Wolfberry Aphid harm provides test data support for botanical pesticide of new generation exploitation efficiently, environmentally friendly, while being into one
Step development and utilization marigold provides scientific basis.
Detailed description of the invention
Fig. 1 is the collection device schematic diagram of marigold volatile matter described in embodiment.
Fig. 2 is the GC-EAD figure of the p- alpha-alpha-dimethyl styrene of marigold volatile component described in embodiment.
Fig. 3 is the mass spectrogram of the p- alpha-alpha-dimethyl styrene of marigold volatile component described in embodiment.
Fig. 4 is the GC-EAD figure of marigold volatile component β-homocyclocitral described in embodiment.
Fig. 5 is the mass spectrogram of marigold volatile component β-homocyclocitral described in embodiment.
Fig. 6 is the GC-EAD figure of marigold volatile component verbenol described in embodiment.
Fig. 7 is the mass spectrogram of marigold volatile component verbenol described in embodiment.
Fig. 8 is the GC-EAD figure of marigold volatile component caryophyllin described in embodiment.
Fig. 9 is the mass spectrogram of marigold volatile component caryophyllin described in embodiment.
Figure 10 is that verbenol described in embodiment influences result figure to Wolfberry Aphid flight behavior.
Figure 11 is verbenol described in embodiment to Wolfberry Aphid odorant-binding protein result figure.
Figure 12 is that verbenol described in embodiment forms influence result figure to alatae.
Figure 13 is that verbenol described in embodiment influences result figure to Wolfberry Aphid population at individual quantity.
Specific embodiment
The present invention is elaborated further below with reference to embodiment.
Embodiment 1, the collection and measurement of Tagetes erecta volatile matter
For examination insect using the Wolfberry Aphid (Aphis sp.) of indoor raising, the fructus lycii on peaceful Xia Zhongning fructus lycii ground will be picked up from
Aphid is seeded on the fructus lycii of chamber planting, removes the aphid of inoculation two days later, selects Individual Size and worm state almost the same
Aptery adult aphid is as test worm
Marigold flower, cauline leaf and whole plant volatile materials sample are collected using headspace analysis.Ten thousand longevity
The collection device schematic diagram of chrysanthemum volatile matter is as shown in Figure 1.Parpark adsorbent is poured into adsorption column, with 0.5ml methylene chloride
(chromatographically pure) elution, elution three times, are then concentrated with High Purity Nitrogen, volatile matter sample of collection to 0.2ml or so.After being concentrated again
Sample with gas-chromatography tentaculum electric potential combined instrument (GC-EAD) analyze volatile matter activity, according to the change on GC-EAD chromatogram
The peak shape and retention time for closing object carry out detailed comparisons, to identify active substance.Marigold different parts (flower, stem
Leaf and whole plant) tentaculum electric potential instrument response value is shown in Table 2.
2 marigold different parts tentaculum electric potential instrument response value of table
| Plant position | Response value (mv) |
| Marigold flower | 0.1069±0.0297a |
| Marigold cauline leaf | 0.1065±0.0271a |
| Whole strain marigold | 0.0481±0.0226a |
As can be drawn from Table 1, marigold different parts volatile matter all has certain influence to the tentaculum electric potential of Wolfberry Aphid,
But it is not significant to influence difference.Therefore, marigold volatile matter sample used in follow-up test is collected in marigold whole plant.
Marigold whole plant volatile constituent analysis (GC-MS): taking marigold whole plant volatile matter sample feeding, into
Sample 2ul, sample introduction mode are Splitless injecting-Sample, and detector temperature is 260 DEG C, and the method that column temperature uses temperature programming, 50 DEG C keep 1
Minute, 120 DEG C of holding 0min are raised to the rate of 5 DEG C/min, 240 DEG C of holding 10min are raised to the rate of 10 DEG C/min, it is total to transport
The row time is 35min.
The measurement result of Tagetes erecta volatile matter is shown in Fig. 2-Fig. 9.Specifically, complex chart 2 and Fig. 3 speculate that marigold is whole
Contain p- alpha-alpha-dimethyl styrene in strain plant volatile.Complex chart 4 and Fig. 5 speculate, in marigold whole plant volatile matter
Contain β-homocyclocitral.Complex chart 6 and Fig. 7 speculate, contain verbenol in marigold whole plant volatile matter.Complex chart
8 and Fig. 9 speculates, contains caryophyllin in marigold whole plant volatile matter.
Embodiment 2, verbenol study Wolfberry Aphid repellent action
Based on embodiment 1, this part primary study verbenol is to Wolfberry Aphid repellent action, specific research contents packet
Including various concentration verbenol influences Wolfberry Aphid flight behavior, to Wolfberry Aphid odorant-binding protein, to Wolfberry Aphid
Alatae is formed and population at individual quantity influences.
Testing resulting data average value ± standard error indicates.Various concentration verbenol escapes to Wolfberry Aphid and goes
It is formed for, alatae and data that population at individual quantity influences carries out variance analysis using the One-way ANOVA of DPS；It is different
Concentration verbenol carries out the significance of difference with Chi-square Test to the odorant-binding protein data of Wolfberry Aphid long range.
(1) various concentration verbenol influences the flight behavior of Wolfberry Aphid
It is divided into two regional observation Wolfberry Aphid escape numbers using transparent ecological box (2.5cm × 2.5cm × 2.5cm), keeps away
Single culture dish or the unsharp problem of mesh enclosure method region division are exempted from.The fructus lycii blade for inhabiting aphid is gently clipped in ecology
In box, the active ingredient solution of 10 μ L various concentrations is dripped respectively on the filter paper of 1cm × 1cm, is propped with toothpick and is placed in ecology
Cassette bottom portion, living body wolfberry leaf on piece folded by ecological box connect 3 almost the same age larvae aphids of 20 body sizes；It is fled from statistics 3min
Aphid number of the blade to the ecological box other end；3 repetitions of each concentration.Experimental temperature is 20 ± 1 DEG C, relative humidity 60 ± 5%.
The results are shown in Figure 10 on the influence of Wolfberry Aphid flight behavior for the verbenol.From fig. 10 it can be seen that
In 0-10 μ g/ μ L concentration range, through analyzing, when verbenene determining alcohol is 0.001 μ g/ μ L and 0.01 μ g/ μ L, Wolfberry Aphid is escaped
Ease behavior is not significant；When 0.1 μ g/ μ L, Wolfberry Aphid flight behavior is significant, and Wolfberry Aphid escapes number with verbenene determining alcohol
Increase and increase.
(2) odorant-binding protein of the Wolfberry Aphid to various concentration verbenol
Using Y-shaped smell sensing apparatus measurement Wolfberry Aphid to the odorant-binding protein of various concentration verbenol.Referring to Du etc.
Y-shaped smell sensing apparatus is designed and produced, Y-shaped smell sensing apparatus is made of the colourless transparent glass pipe of the long 10cm of 3 arms, internal diameter 2cm, two-arm of ventilating
60 ° of angle, atmosphere Acquisition Instrument successively enters two-arm through active carbon taste source bottle and flowmeter as air pump, air.Experiment periods compartment
Interior temperature is 20 ± 1 DEG C, air velocity 100mL/min, and relative humidity is 60 ± 5%, places 2 above two-arm at 30cm
12W fluorescent lamp, the light to ensure Y-piece two-arm is uniform, reduces the influence to aphid behavior reaction.
Filter paper containing verbenene alcoholic solution and control (being free of verbenene alcoholic solution) is respectively placed in the bottle of taste source,
Experiment is successively carried out from low concentration to high concentration, and aphid is discharged at the position glass tube base portion l/2, records aphid in 5min
Reaction reaches an arm and is considered as reaction when being more than 5cm, on the contrary then be considered as and do not react.Every test is primary, 5 exchange two-arm of every survey,
And Y type glass tube is wiped with dehydrated alcohol.Each concentration repeats 3 groups, and every group of 20-22 head measures 60 3 age larvae aphids altogether.It is each
Clean all glass tube and connection rubber tube after concentration tests with dehydrated alcohol and distilled water respectively.
Wolfberry Aphid is as shown in figure 11 to the odorant-binding protein of various concentration verbenol.It can be seen from figure 11 that
Verbenene determining alcohol is in 0.001 μ g/ μ L and 0.01 μ g/ μ L, and with sucking action but not significant to Wolfberry Aphid, concentration reaches
0.1 μ g/ μ L has significant repellent action, and increases with concentration, increases the repellent quantity of Wolfberry Aphid.
(3) influence of the various concentration verbenol to the alatae formation and population at individual quantity of Wolfberry Aphid
With reference to the method for Kunert etc., 15 1 age larvae aphids are inoculated on fructus lycii seedling, 10 μ L verbenene alcoholic solutions are dripped
Be added on filter paper, be fixed on fructus lycii basin center with toothpick, daily timing be added dropwise 5 times (time for adding is set as 9:00,11:30,
14:00,16:30 and 19:00), 5d is persistently handled, the death toll of larvae aphid is observed and recorded daily, records it after being developed to adult aphid
Wing, and record 2 weeks after aphid total quantity.Each concentration is repeated 5 times.
Figure 12 is shown in the influence that various concentration verbenol forms alatae, it can be recognized from fig. 12 that various concentration horse
The influence that whip oxalene alcohol forms alatae is not significant, when verbenene determining alcohol reaches 10 μ g/ μ L, aphid death rate mistake
The formation number of height, alatae significantly reduces.
Figure 13 is shown in influence of the various concentration verbenol to Wolfberry Aphid population at individual quantity, as can be seen from Figure 13,
In 0-10 μ g/ μ L concentration range, through analyzing, when verbenene determining alcohol is 0.001 μ g/ μ L and 0.01 μ g/ μ L, to fructus lycii
The influence of aphis population individual amount is not significant；When verbenene determining alcohol reaches 0.1 μ g/ μ L, aphis population can be significantly inhibited
Individual amount, and increase inhibiting effect with concentration and increase.
Embodiment 3, the controlled release formulation of capsule preparation of the volatile matter containing marigold and its control efficiency evaluation to Wolfberry Aphid
(1) preparation of controlled release formulation of capsule
Respectively with the p- alpha-alpha-dimethyl styrene of cycloheptaamylose inclusion marigold volatile matter, β-homocyclocitral, horsewhip
Oxalene alcohol, caryophyllin are prepared into marigold volatile matter cycloheptaamylose inclusion compound.The preparation process description of this inclusion compound is such as
Under:
Precise cycloheptaamylose, is placed in glass beaker, adds a certain amount of distilled water, is configured to saturated solution；With
The sufficiently miscible marigold volatile matter of the 3-5 times of dehydrated alcohol measured opens stirring when saturated solution temperature near fixed temperature
Device is slowly dropped into configured marigold volatile matter solution while stirring, takes out after being added dropwise to complete rear maintenance stirring；Inclusion
Cycloheptaamylose solution, which is cooled to room temperature, to be placed on refrigerating in 4 DEG C of refrigerators, for 24 hours decompression extracting afterwards, uses nothing in extractive process
Water-ethanol washs 3 times, until the marigold volatile matter that inclusion compound surface does not include is cleaned.The inclusion compound obtained after extracting is put
In 50 DEG C of freeze-day with constant temperature baking ovens, 4h is placed, marigold volatile matter cycloheptaamylose inclusion compound can be obtained.
Using marigold volatile matter cycloheptaamylose inclusion compound obtained as the prevention and treatment active principle of Wolfberry Aphid, referring to existing
Technology be prepared into respectively 3% p- alpha-alpha-dimethyl styrene controlled release formulation of capsule, 3% β-homocyclocitral controlled release formulation of capsule,
3% verbenol controlled release formulation of capsule, 3% caryophyllin controlled release formulation of capsule.
(2) the control efficiency evaluation of Wolfberry Aphid
With above-mentioned 3% p- alpha-alpha-dimethyl styrene controlled release formulation of capsule, 3% β-homocyclocitral controlled release formulation of capsule,
3% verbenol controlled release formulation of capsule, 3% caryophyllin controlled release formulation of capsule are as reagent agent, and pyrethrin is as control
Medicament.
Insect population is investigated before application on July 25th, 2018, investigates within 1 day residual borer population after July 26 medicine, 3 after July 28 medicine
It investigates residual borer population, investigates within 7 days residual borer population after August medicine on the 1st, investigates within 14 days residual borer population after August medicine on the 8th, 21 days after August medicine on the 15th
Residual borer population test is investigated, is investigated 6 times altogether.According to the investigation method of provincial standard " DB64/T852-2013 ", every cell is taken out at random
5 branches are taken, the population number of Wolfberry Aphid within the scope of the every branch top tip 30cm of investigation records.
Pilot survey result calculates control efficiency as follows, and answers grade using new to result in DPS statistical software
Difference method is for statistical analysis and significance test.Above-mentioned medicament is shown in Table 3 to the control efficiency statistical result of Wolfberry Aphid.
Calculation formula:
In formula: PT0Borer population before chemicals treatment district medicine；PT1Borer population after chemicals treatment district medicine；CK0Blank district medicine
Preceding borer population；CK1Borer population after blank district medicine.
Control efficiency of the aphid prevention and control composition of 3 volatile matter containing marigold of table to Wolfberry Aphid
Table 3 is the results show that 3% p- alpha-alpha-dimethyl styrene controlled release formulation of capsule, 3% β-homocyclocitral microcapsules
Sustained release agent, 3% verbenol controlled release formulation of capsule, 3% caryophyllin controlled release formulation of capsule and pyrethrin are to Wolfberry Aphid
With certain control efficiency, it can be used for the prevention and treatment of Wolfberry Aphid.3 days after medicine, pyrethrin imitates the prevention and treatment of Wolfberry Aphid
Fruit is preferable, is superior to marigold volatile matter.In marigold volatile matter controlled release formulation of capsule, 1 day after medicine, the micro- glue of verbenol
The control efficiency of capsule sustained release agent is suitable with pyrethrin, and is substantially better than other marigold volatile matters；14 days after medicine, whip oxalene alcohol
The control efficiency of controlled release formulation of capsule still can reach 65.02%；21 days after medicine, the control efficiency of whip oxalene alcohol controlled release formulation of capsule
It is suitable with p- alpha-alpha-dimethyl styrene, it is superior to other marigold volatile matters and pyrethrin.
Further narration is done to the present invention above in conjunction with embodiment, but present invention is not limited to the embodiments described above,
Within the knowledge of one of ordinary skill in the art, it can also make without departing from the purpose of the present invention
Various change.
Claims (7)
1. the aphid prevention and control composition of the volatile matter containing marigold, which is characterized in that the composition includes having to walk quickly and keep away to aphid
The marigold volatile component of effect, the marigold volatile component are p- alpha-alpha-dimethyl styrene, the high lemon of β-ring
Any one of aldehyde, verbenol, caryophyllin.
2. the aphid prevention and control composition of the volatile matter containing marigold according to claim 1, which is characterized in that the marigold is waved
Hair object is collected in the whole strain plant of flower, cauline leaf or marigold of marigold.
3. the aphid prevention and control composition of the volatile matter containing marigold according to claim 1, which is characterized in that the marigold is waved
Hair object can influence the tentaculum electric potential of aphid.
4. the aphid prevention and control composition of the volatile matter containing marigold according to claim 1, which is characterized in that the marigold is waved
It sends out object component and selects verbenol, the verbenol walks quickly and keeps away the use concentration of Wolfberry Aphid not less than 0.1 μ g/ μ L.
5. the aphid prevention and control composition of the volatile matter containing marigold according to claim 1, is prepared into the agent agriculturally used
Type.
6. the aphid prevention and control composition of the volatile matter containing marigold according to claim 5, which is characterized in that the dosage form is micro-
Capsule sustained-release.
7. application of the aphid prevention and control composition of the volatile matter containing marigold described in claim 1 on prevention and treatment Wolfberry Aphid.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910202924.2A CN109819982B (en) | 2019-03-18 | 2019-03-18 | Aphid prevention and control composition containing marigold volatile matter and application thereof |
| CN202110163736.0A CN112741086B (en) | 2019-03-18 | 2019-03-18 | Application of marigold volatile beta-cyclohomocitral to control of medlar aphids |
| CN202110164502.8A CN112956476B (en) | 2019-03-18 | 2019-03-18 | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
| CN202110164499.XA CN112772644B (en) | 2019-03-18 | 2019-03-18 | Application of marigold volatile substance to alpha-dimethyl styrax for preventing and treating medlar aphid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910202924.2A CN109819982B (en) | 2019-03-18 | 2019-03-18 | Aphid prevention and control composition containing marigold volatile matter and application thereof |
Related Child Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110164502.8A Division CN112956476B (en) | 2019-03-18 | 2019-03-18 | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
| CN202110163736.0A Division CN112741086B (en) | 2019-03-18 | 2019-03-18 | Application of marigold volatile beta-cyclohomocitral to control of medlar aphids |
| CN202110164499.XA Division CN112772644B (en) | 2019-03-18 | 2019-03-18 | Application of marigold volatile substance to alpha-dimethyl styrax for preventing and treating medlar aphid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109819982A true CN109819982A (en) | 2019-05-31 |
| CN109819982B CN109819982B (en) | 2021-10-12 |
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| CN202110164502.8A Active CN112956476B (en) | 2019-03-18 | 2019-03-18 | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
| CN201910202924.2A Active CN109819982B (en) | 2019-03-18 | 2019-03-18 | Aphid prevention and control composition containing marigold volatile matter and application thereof |
| CN202110163736.0A Active CN112741086B (en) | 2019-03-18 | 2019-03-18 | Application of marigold volatile beta-cyclohomocitral to control of medlar aphids |
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| CN202110164502.8A Active CN112956476B (en) | 2019-03-18 | 2019-03-18 | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
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| KR101301110B1 (en) * | 2004-09-24 | 2013-08-27 | 짐리즈 게엠베하 운트 캄파니 카게 | Use of Aroma Substance Mixtures as Agents against Bad Breath |
| CN105779130A (en) * | 2016-04-29 | 2016-07-20 | 湖北中烟工业有限责任公司 | Method for preparing marigold volatile oil by fermentation and application of marigold volatile oil |
| CN107711223A (en) * | 2017-10-19 | 2018-02-23 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | The preventing control method of Wolfberry Aphid |
| CN108271815B (en) * | 2017-11-29 | 2022-07-26 | 山东农业大学 | Method for preventing and treating aphids of chrysanthemum by marigold extract |
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| CN112772644B (en) | 2021-08-17 |
| CN112741086B (en) | 2021-10-26 |
| CN112772644A (en) | 2021-05-11 |
| CN112956476B (en) | 2022-04-01 |
| CN109819982B (en) | 2021-10-12 |
| CN112956476A (en) | 2021-06-15 |
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