CN105779130A - Method for preparing marigold volatile oil by fermentation and application of marigold volatile oil - Google Patents
Method for preparing marigold volatile oil by fermentation and application of marigold volatile oil Download PDFInfo
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- CN105779130A CN105779130A CN201610278233.7A CN201610278233A CN105779130A CN 105779130 A CN105779130 A CN 105779130A CN 201610278233 A CN201610278233 A CN 201610278233A CN 105779130 A CN105779130 A CN 105779130A
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- volatile oil
- marigold
- tagetis erectae
- fermentation
- beta
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- 239000012159 carrier gas Substances 0.000 description 1
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 description 1
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 description 1
- 235000007746 carvacrol Nutrition 0.000 description 1
- 229940117948 caryophyllene Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- WTWBUQJHJGUZCY-UHFFFAOYSA-N cuminaldehyde Chemical compound CC(C)C1=CC=C(C=O)C=C1 WTWBUQJHJGUZCY-UHFFFAOYSA-N 0.000 description 1
- 125000000753 cycloalkyl group Chemical group 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- QYDYPVFESGNLHU-UHFFFAOYSA-N elaidic acid methyl ester Natural products CCCCCCCCC=CCCCCCCCC(=O)OC QYDYPVFESGNLHU-UHFFFAOYSA-N 0.000 description 1
- 150000002085 enols Chemical class 0.000 description 1
- CAMHHLOGFDZBBG-UHFFFAOYSA-N epoxidized methyl oleate Natural products CCCCCCCCC1OC1CCCCCCCC(=O)OC CAMHHLOGFDZBBG-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- LTUMRKDLVGQMJU-UHFFFAOYSA-N famesylacetone Natural products CC(C)=CCCC(C)=CCCC(C)=CCCC(C)=O LTUMRKDLVGQMJU-UHFFFAOYSA-N 0.000 description 1
- LTUMRKDLVGQMJU-IUBLYSDUSA-N farnesyl acetone Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CCC(C)=O LTUMRKDLVGQMJU-IUBLYSDUSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- DUYRYUZIBGFLDD-UHFFFAOYSA-N gamma-gurjunene Natural products CC1CCC(C(C)=C)C=C2C(C)CCC21 DUYRYUZIBGFLDD-UHFFFAOYSA-N 0.000 description 1
- HIGQPQRQIQDZMP-UHFFFAOYSA-N geranil acetate Natural products CC(C)=CCCC(C)=CCOC(C)=O HIGQPQRQIQDZMP-UHFFFAOYSA-N 0.000 description 1
- HIGQPQRQIQDZMP-DHZHZOJOSA-N geranyl acetate Chemical compound CC(C)=CCC\C(C)=C\COC(C)=O HIGQPQRQIQDZMP-DHZHZOJOSA-N 0.000 description 1
- YDLBHMSVYMFOMI-SDFJSLCBSA-N germacrene Chemical compound CC(C)[C@H]1CC\C(C)=C\CC\C(C)=C\C1 YDLBHMSVYMFOMI-SDFJSLCBSA-N 0.000 description 1
- 229930001612 germacrene Natural products 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 235000018219 lavender Nutrition 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229930007503 menthone Natural products 0.000 description 1
- 125000005911 methyl carbonate group Chemical class 0.000 description 1
- 229940073769 methyl oleate Drugs 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000000199 molecular distillation Methods 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 150000002773 monoterpene derivatives Chemical class 0.000 description 1
- 235000002577 monoterpenes Nutrition 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 229930007459 p-menth-8-en-3-one Natural products 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 238000001256 steam distillation Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 229930192474 thiophene Natural products 0.000 description 1
- 239000010678 thyme oil Substances 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
- A24B15/303—Plant extracts other than tobacco
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
Abstract
The invention provides a method for preparing marigold volatile oil by beta-glucosidase fermentation. The method includes the following steps: (1) mixing marigold flowers and water in the mass ratio of 1:10-40, regulating the pH to 4-6 with acetic acid, equilibrating at the temperature of 45-55 DEG C for 30-60 minutes, adding beta-glucosidase accounting for 0.1-0.6% of the mass ratio of the marigold flowers, performing even mixing and continuous stirring, and incubating at the temperature of 45-55 DEG C for 12-48 hours; (2) distilling with water vapor for 5-10 hours to extract volatile oil, and subjecting obtained yellowish green oily substances to enzyme fermentation to extract the marigold volatile oil. The method for extracting the volatile oil by beta-glucosidase fermentation of the marigold flowers has the advantages that aroma is concentrated, and volatile substances bringing stimulus and offensive odor are eliminated or reduced; by application of the marigold volatile oil in reconstituted tobacco, the defects that the marigold volatile oil not subjected to enzyme fermentation in the reconstituted tobacco is discordant with smoke, coarse, high in irritation and heavy in offensive odor and covers up the tobacco flavor are overcome.
Description
Technical field
The present invention relates to tobacco aromatics using technical field, Flos Tagetis Erectae volatilization is prepared in specifically a kind of fermentation
The method of oil and the application in reconstituted tobacco perfuming thereof.
Background technology
Reconstituted tobacco, mainly by offal, fragment, cabo or discarded tobacco leaf add adhesive and its
His additives etc. form, and there is miscellaneous QI weight, zest is big and mouthfeel is uncomfortable etc. inhales taste defect, shadow
The using effect in cigarette product and addition are rung.The way of additive is generally used to enter
Row improves, and applying more additive at present is Nicotiana tabacum L., natural plant kind extract or volatile oil
Deng, these extracts are typically obtained by water, alcohol extraction, vapor distillation or molecular distillation,
Improvement limitation to cigarette quality after interpolation, the most irritant big, miscellaneous QI is many, fragrance not
The defects such as foot.
Flos Tagetis Erectae (Tagets erecta L) is Compositae Tagetes annual herb plant, orange
Kind dried marigold flower piece carotenoid content is 0.6%～2.5%, and wherein lutein ester is about
Account for total carotinoid 88%～92%.Flos Tagetis Erectae quintessence oil, intends, in order to modulate, the perfume (or spice) that odor type is graceful
Water, floral water and production fragrance medical-type cosmetics.Research for Flos Tagetis Erectae at present focuses mostly on
In phylloxanthin aspect, the chemical composition for quintessence oil also has certain research, and Flos Tagetis Erectae quintessence oil has
There are antioxidation, anophelifuge, the biological activity such as antibacterial.It is reported Folium Tagetis Erectae volatile oil water steam
The extraction ratio of steam distillation method has 0.6-0.9%.
The present inventor finds under study for action: that distributes Studies of The Aromatic Substances in the middle part of natural plant spice sends out perfume
Group tends to other the most low molecular saccharides of composition in plant cell etc. with chemical bond
The form closed combines, thus inhibits the distributing and be difficult to be extracted, in spice science of fragrance
In field, such material is referred to as " fragrance presoma ".These materials itself British plain spirits and not
Volatilization, if but after selecting suitable enzyme to be hydrolyzed, releasably go out strong to there is natural feature
The material of fragrance.Beta-glucosidase can hydrolyze the β-D-being incorporated into end irreducibility
Glucoside bond, discharges β-D-Glucose and corresponding aglucon simultaneously.Additionally, β-Portugal
Flavor precursors in fruit, vegetables, tea can be hydrolyzed to have strong natural flavour mountaineous by polyglycoside enzyme
Aroma substance, moreover it is possible to degraded or Synergistic degradation cellulose.
In prior art, with marigold flower as raw material, beta-glucosidase enzyme fermentation prepared for ten thousand longevity
Chrysanthemum volatile oil, improves extraction ratio and flavouring essence quality, and perfumed applications in reconstituted tobacco, improves
The aroma quality of flue gas, mouthfeel, reduce to stimulate and reduce miscellaneous QI, and there is not been reported.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is provided that prepared by a kind of beta-glucosidase enzyme fermentation
Flos Tagetis Erectae volatile oil, make the flavor precursor of marigold flower through the effect of enzyme produce degraded, decompose,
It is converted into the aroma substance of molecule that enrich, little, and the material increasing excitement and miscellaneous QI contains
Amount minimizing even disappears, and improves the extraction ratio of Flos Tagetis Erectae volatile oil；And this fermentation ten thousand longevity
Chrysanthemum volatile oil is being applied in reconstituted tobacco, and abundant fragrance, promoting or transferring cigarette are fragrant, mouthfeel, reduce thorn
Swash, reduce miscellaneous QI etc..
The present invention provides a kind of method that beta-glucosidase enzyme fermentation prepares Flos Tagetis Erectae volatile oil,
Comprise the following steps:
(1) being mixed with water 1:10 in mass ratio～40 by marigold flower, pH to 4～6 adjusted by acetic acid,
Balance 30-60 minute under the conditions of 45～55 DEG C, add and account for marigold flower mass ratio 0.1～0.6%
Beta-glucosidase, mix homogeneously does not stop stirring, 45～55 DEG C of incubations 12～48 hours；
(2) vapor distillation 5～10 hours extraction volatile oil, gained yellow green grease is enzyme
Fermented extracted Flos Tagetis Erectae volatile oil.
The present invention provides Flos Tagetis Erectae volatile oil prepared by a kind of said method in reconstituted tobacco
Application, it is 0.01～0.03% that described Flos Tagetis Erectae volatile oil accounts for the mass ratio of reconstituted tobacco.
The volatile oil that beta-glucosidase enzyme fermentation Flos Tagetis Erectae is prepared by the present invention with without enzyme fermentation
Flos Tagetis Erectae volatile oil comparison (hereinafter referred to as comparison) carry out volatility aroma component GC/MS
Relative analysis.
(1) volatile oil processes: by volatile oil dichloromethane extraction, dilution 100 times, take
1～2mL is used for carrying out GC/MS analysis.
(2) GC/MS analyzes: HP-5MS (50m × 0.25mm × 0.25 μm) capillary column；
Injector temperature: 250 DEG C；Split ratio 20:1；Carrier gas: He, 1mL/min；Heating schedule:
It is raised to 250 DEG C (5min) with 5 DEG C/min from 50 DEG C (1min)；Transmission line temperature: 250 DEG C；
Ion source: EI source；Electron energy: 70eV；Sweep limits: 50～550amu；Use WILEY
Retrieve with MINILAB spectrum storehouse.
(3) volatile ingredient relative analysis
GC/MS analysis result (table 1) shows,
1. the Flos Tagetis Erectae volatile oil without enzyme fermentation process is divided into from identifying 98 kinds of volatility
Fragrance component, mainly includes alkenes, terpenes, cyclic hydrocarbon compounds of group, terpenoid, alcohols, ketone
Class, aldehydes, alkanes, also the heterocycle compound such as furan, pyrazine, thiophene, accounts for 74.61%,
Wherein limonene (5.02%), 4 carenes (5.5%), spathulenol (8.12%), caryophyllene
Oxide (4.13%), myristic acid (6.78%), Palmic acid (6.10%) etc., account for 35.65%；
2. it is divided into from identifying in Flos Tagetis Erectae volatile oil prepared by beta-glucosidase enzyme fermentation
127 kinds of aroma volatile, percentage contents accounts for 95.04%, wherein limonene
(3.61%), 4-carene (4.62%), β-caryophyllene (16.82%), spathulenol (11.03%),
Caryophyllene oxide (2.25%), myristic acid (5.90%), Palmic acid (5.98%) etc.,
Account for 50.21%；Enzymatically after the raw perfume (or spice) of fermentation, the fragrance component in Flos Tagetis Erectae volatile oil is more rich,
It is converted to new fragrance component β-caryophyllene, and account for always with other 6 kinds of fragrance components
More than the 50% of volatile ingredient, has concentrated advantage fragrance, constitutes Enzyme treatment Flos Tagetis Erectae and waves
The characteristic perfume of hair oil, improves flavouring essence quality；
3. in Flos Tagetis Erectae volatile oil prepared by beta-glucosidase enzyme fermentation, newly occur in that 70 kinds of perfume (or spice)
Gas composition, mainly have β-caryophyllene (16.818%), β-phellandrene, citral, 4,7-bis-
Methyl benzofuran, Eucarvone, E-carveol, carvone, thymol, (+)-α-
Longipinene, α-cubebene, (+)-aromadendrene, 2,3-dihydro-2-methoxy benzofuran,
Dicyclo germacrene, dihydro-(-) neoclovene-(I), o-Herba Menthae-8-alkene, loudspeaker
Tea alcohol, peach aldehyde, farnesyl acetone, fourth position nonalactone, methyl linolenate, stearic acid etc..
Wherein, β-caryophyllene (16.818%) is a kind of dicyclo sesquiterpenoids, have Xin Xiang,
The Radix Aucklandiae, Citrus are fragrant, Camphora fragrant, gentle Flos Caryophylli fragrance, have relieving asthma, antitussive, phlegm-dispelling functions；
Oleum Eucalypti enol (11.031%) naturally occurs in multiple fragrance of a flower oil；β-phellandrene tool Citrus,
The refrigerant fragrance of Fructus Piperis；Citral has citris aromas；Carvone is a kind of important spice, it
Being widely used in food industry and chemical industry, particularly toothpaste, hard sugar, chewing gum is with each
In kind of beverage, therefore (-) synthesis of-carvone has important economic worth；Thymol is
One monoterpene phenol, pungent perfume (or spice) and hay-scented fragrance as having thyme oil；(+)-α-longipinene,
α-cubebene, (+)-aromadendrene, spathulenol be terpene compound, has fragrance；
Peach aldehyde has strong Fructus Persicae and Semen Armeniacae Amarum sample fragrance, is a kind of important lactone spice；Acacia farnesiana Willd. base
Acetone has the fragrant and sweet gas of light green grass or young crops, increases blue or green fragrant and sweet and flue-cured tobacco fragrant；Fourth position nonalactone have sweet,
Warm nut, milk, butter, Cortex cocois radicis sample gas taste；Methyl linolenate is unsaturated fatty acid,
It is the important substance constituting Medicated cigarette fragrance, soft flue gas, alleviate stimulation；Stearic acid gives Nicotiana tabacum L.
Fat and fat cured sample fragrance；
4. in Flos Tagetis Erectae volatile oil prepared by beta-glucosidase enzyme fermentation Borneolum Syntheticum, menthol,
Methyl phenylacetate, trimethylbenzene methanol, Zang Honghua aldehyde, menthone epoxide, geranyl
Ester, methyl laurate, lauric acid, methyl myristate, 15 methyl carbonates, plant ketone,
Containing of the compositions such as methyl hexadecanoate, benzo (a) azophenlyene, methyl linoleate, methyl stearate
Measure to have and improve to some extent.The wherein refrigerant spine of Borneolum Syntheticum fragrance, micro-strip medicated incense, Fructus Piperis perfume,
Fragrance wafts；Menthol has Herba Menthae fragrance and has refrigerant effect；Methyl phenylacetate naturally occurs
In cocoa, coffee and Fructus Fragariae Ananssae, there are weak Mel and Moschus sample fragrance, slightly sweet taste；Zang Hong
Flower aldehyde all has obvious inhibiting effect to multiple cancerous cell, has the Radix Aucklandiae, Xin Xiang, medicated incense, powder perfume；
Geranyl acetate has Flos Rosae Rugosae and Garden lavender fragrance；Methyl laurate has similar aroma and the fragrance of a flower；
Lauric acid is micro-laurel fat fragrance；Methyl myristate has like Mel and the fragrance of Rhizoma Iridis Tectori sample；Sub-
Methyl oleate is unsaturated fatty acid, is the important substance constituting Medicated cigarette fragrance, soft flue gas,
Alleviate stimulation；
5., after enzyme fermentation, some compositions in Flos Tagetis Erectae volatile oil disappear or content reduces: Fructus Citri Limoniae
Alkene, 2,3-dihydro-2-methyl benzofuran, gum camphor, (-)-4-terpinol, pulegone,
2-acetyl group-3,5-dimethyl pyrazine, cumal, D-CARVONE, bromoacetic acid phenethyl ester,
(1S)-(+)-3-carene, carvacrol, 1,2-dihydro-1,1,6-trimethyl-naphthalene, (-)-α-gurjunene,
α-ionone, 4-(2,6,6-trimethyl-1,3-cyclohexadiene)-3-butene-2-one, Valencia
Fructus Citri tangerinae alkene, β-Se Lin alkene, Rhizoma Acori Calami alkene epoxide, caryophyllene ethanol, caryophyllene oxide,
Heterocyclic alkene and the heterocyclic compounds such as 3,7,7-trimethyl-bicyclo [4.1.0] hept-2-ene", it may be possible to
Under the effect of beta-glucosidase, some composition there occurs that structure change defines isomerism
Body, and the fracture of the macromolecular substances such as heterocyclic alkene, heterocyclic compound is decomposed, degraded, produces more
Many little molecule aroma substances or change into other aroma volatile and even disappear, may
Be conducive to the raising of cigarette quality.
In a word, from volatilization, semi-volatility component change in terms of, through beta-glucosidase enzyme fermentation
Flos Tagetis Erectae volatile oil perfuming effect in reconstituted tobacco may be better than comparison.
The volatile ingredient GC/MS contrast that table 1 beta-glucosidase fermented extracted Flos Tagetis Erectae volatile oil compares with it
In table 1, symbol "/" represents and does not detects；Inside it is designated as phenylethyl propionate.
(4) the sensory evaluation analysis of perfuming in reconstituted tobacco
Show, through beta-glucosidase enzyme fermentation through the test (table 2) of smokeing panel test of reconstituted tobacco perfuming
The Flos Tagetis Erectae volatile oil of preparation perfuming effect in reconstituted tobacco is significantly better than non-ferment treatment
Flos Tagetis Erectae volatile oil (is called for short comparison), and odor characteristic shows the fullest, highlights, fragrance
Abundant, aroma quality improves, the softer exquisiteness of flue gas, and zest, miscellaneous QI substantially reduce, band
There is refrigerant time sweet sense.Suitable perfuming amount is 0.01%.
Table 2 enzyme fermentation Flos Tagetis Erectae volatile oil and control sample perfuming smoking result in reconstituted tobacco thereof
The invention have the advantage that and extract volatile oil through beta-glucosidase enzyme fermentation marigold flower,
The aroma component changes of its volatile oil is big, and fragrance is more rich, aroma quality improves, characteristic perfume collection
In, bring the volatile material stimulated with miscellaneous QI disappear or reduce, macromolecular substances is enzymatically given birth to
Fragrant degrade, decompose, change into little molecule aroma substance and even disappear, being converted into dividing of having is different
Structure body；Reconstituted tobacco is applied, there is coordination flue gas, increase fresh and sweet refrigerant sense, reduction thorn
Swash, reduce miscellaneous QI, improve the advantage inhaled taste, strengthen throat's comfortableness, can be fragrant with other cigarette
Essence spice mixing preparation uses, and improves the Flos Tagetis Erectae volatile oil without enzymolysis in reconstituted tobacco
Defect inharmonious with flue gas, that coarse, zest is big, miscellaneous QI is heavy, to cover cigarette fragrant.The method
Raw materials used wide material sources, instrument is simple, it is easy to operate and realize industrialization, and low cost is green
Colour circle is protected, it is intended to the application developing natural tobacco aromatics using for Enzyme treatment provides reference.
Detailed description of the invention
A part of embodiment is set forth below the relevant technical problem of the present invention is made the most in detail
Description, it is necessary to it is pointed out here that specific examples below is simply to the present invention the most furtherly
Bright, do not represent limiting the scope of the invention.Other people make according to the present invention some
Nonessential amendment and adjustment, still fall within protection scope of the present invention.
Embodiment 1
(1) being mixed with water 1:10 in mass ratio by Flos Tagetis Erectae coarse powder, pH to 4.5 adjusted by acetic acid,
Under the conditions of 45 DEG C, balance half an hour, add and account for Flos Tagetis Erectae meal quality β-Fructus Vitis viniferae than 0.1%
Glycosidase mix homogeneously, does not stop stirring, 45 DEG C of incubations 12 hours；
(2) vapor distillation extracts volatile oil in 5 hours, and gained yellow green grease is that enzyme is sent out
Ferment Flos Tagetis Erectae volatile oil；
Above-mentioned Flos Tagetis Erectae enzyme fermentation is prepared volatile oil perfuming in reconstituted tobacco, accounts for reconstituted tobacco
Mass ratio be 0.01%.
Sensory evaluating smoking's effect: preferable with flue gas harmony, has fresh and sweet refrigerant sense, flue gas is soft,
Stimulating reduction, miscellaneous QI minimizing, pleasant impression totally, aroma quality improves.
Embodiment 2
(1) being mixed with water 1:20 in mass ratio by Flos Tagetis Erectae coarse powder, pH to 5.0 adjusted by acetic acid,
Balance 40 minutes under the conditions of 50 DEG C, add and account for Flos Tagetis Erectae meal quality β-Portugal than 0.3%
Polyglycoside enzyme mix homogeneously, does not stop stirring, 50 DEG C of incubations 18 hours；
(2) vapor distillation extracts volatile oil in 6 hours, and gained yellow green grease is enzymatic
Raw fragrant Flos Tagetis Erectae volatile oil；
By the above-mentioned Flos Tagetis Erectae volatile oil prepared through beta-glucosidase enzyme fermentation in reconstituted tobacco
Perfuming, the mass ratio accounting for reconstituted tobacco is 0.01%.
Sensory evaluating smoking's effect: good with flue gas harmony, fragrance is more rich, aroma quality improves, larynx
Portion is comfortable, and the soft exquisiteness of flue gas has refrigerant time sweet sense, stimulates and substantially reduces, and miscellaneous QI reduces, remaining
Taste is clean.
Embodiment 3
(1) being mixed with water 1:40 in mass ratio by Flos Tagetis Erectae coarse powder, pH to 6 adjusted by acetic acid,
Balance 45 minutes under the conditions of 55 DEG C, add and account for Flos Tagetis Erectae meal quality β-Portugal than 0.5%
Polyglycoside enzyme mix homogeneously, does not stop stirring, 55 DEG C of incubations 32 hours；
(2) vapor distillation extracts volatile oil in 10 hours, and gained yellow green grease is enzymatic
Raw fragrant Flos Tagetis Erectae volatile oil；
By the above-mentioned Flos Tagetis Erectae volatile oil prepared through beta-glucosidase enzyme fermentation in reconstituted tobacco
Perfuming, the mass ratio accounting for reconstituted tobacco is 0.01%.
Sensory evaluating smoking's effect: good with flue gas harmony, there is refrigerant sweet sense in throat, comfortable, flue gas
Soft, stimulation reduces, miscellaneous QI reduces, pleasant impression is clean, and aroma quality improves.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is also
Being not limited to this, any those skilled in the art of belonging to are at the technology model that the invention discloses
In enclosing, the change that can readily occur in or replacement, all should contain within protection scope of the present invention.
Therefore, protection scope of the present invention should be as the criterion with scope of the claims.
Claims (2)
1. the method that beta-glucosidase enzyme fermentation prepares Flos Tagetis Erectae volatile oil, its feature
It is to comprise the following steps:
(1) being mixed with water 1:10 in mass ratio～40 by marigold flower, pH to 4～6 adjusted by acetic acid,
Balance 30-60 minute under the conditions of 45～55 DEG C, add and account for marigold flower mass ratio 0.1～0.6%
Beta-glucosidase, mix homogeneously does not stop stirring, 45～55 DEG C of incubations 12～48 hours；
(2) vapor distillation 5～10 hours extraction volatile oil, gained yellow green grease is enzyme
Fermented extracted Flos Tagetis Erectae volatile oil.
2. the Flos Tagetis Erectae volatile oil that prepared by a method as claimed in claim 1 is at reconstituted tobacco
In application, it is 0.01～0.03% that described Flos Tagetis Erectae volatile oil accounts for the mass ratio of reconstituted tobacco.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610278233.7A CN105779130A (en) | 2016-04-29 | 2016-04-29 | Method for preparing marigold volatile oil by fermentation and application of marigold volatile oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610278233.7A CN105779130A (en) | 2016-04-29 | 2016-04-29 | Method for preparing marigold volatile oil by fermentation and application of marigold volatile oil |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190555A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of cigarette Flos Tagetis Erectae spice |
| CN106281699A (en) * | 2016-08-16 | 2017-01-04 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Flos Tagetis Erectae quintessence oil and processing method |
| CN106617273A (en) * | 2016-10-12 | 2017-05-10 | 湖北中烟工业有限责任公司 | Clove mouth tobacco product and preparation method of clove mouth tobacco product |
| CN106978258A (en) * | 2017-05-24 | 2017-07-25 | 湖北中烟工业有限责任公司 | Enzymatic flavouring improves the preparation method and application of the FLOS CHRYSANTHEMI ALBA from Haizhou of China volatile oil of flavouring essence quality |
| CN108315352A (en) * | 2018-01-31 | 2018-07-24 | 湖北中烟工业有限责任公司 | A kind of Enzyme treatment prepares the method and its application of lavender oil |
| CN112956476A (en) * | 2019-03-18 | 2021-06-15 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651056A (en) * | 2015-03-12 | 2015-05-27 | 安吉依思味食品有限公司 | Method for preparing tea tree flower essential oil |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104651056A (en) * | 2015-03-12 | 2015-05-27 | 安吉依思味食品有限公司 | Method for preparing tea tree flower essential oil |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190555A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of cigarette Flos Tagetis Erectae spice |
| CN106281699A (en) * | 2016-08-16 | 2017-01-04 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Flos Tagetis Erectae quintessence oil and processing method |
| CN106617273A (en) * | 2016-10-12 | 2017-05-10 | 湖北中烟工业有限责任公司 | Clove mouth tobacco product and preparation method of clove mouth tobacco product |
| CN106978258A (en) * | 2017-05-24 | 2017-07-25 | 湖北中烟工业有限责任公司 | Enzymatic flavouring improves the preparation method and application of the FLOS CHRYSANTHEMI ALBA from Haizhou of China volatile oil of flavouring essence quality |
| CN108315352A (en) * | 2018-01-31 | 2018-07-24 | 湖北中烟工业有限责任公司 | A kind of Enzyme treatment prepares the method and its application of lavender oil |
| CN112956476A (en) * | 2019-03-18 | 2021-06-15 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Application of caryophyllin, volatile of marigold, in preventing and treating medlar aphid |
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