CN112674035A - Ladybug larva releaser and use method thereof - Google Patents
Ladybug larva releaser and use method thereof Download PDFInfo
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- CN112674035A CN112674035A CN202011594857.2A CN202011594857A CN112674035A CN 112674035 A CN112674035 A CN 112674035A CN 202011594857 A CN202011594857 A CN 202011594857A CN 112674035 A CN112674035 A CN 112674035A
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- ladybug
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Abstract
The invention discloses a ladybug larva releaser and a use method thereof, wherein the ladybug larva releaser comprises a box body and a box cover, and the box body and the box cover are movably connected; the side wall of the box body is provided with a plurality of air holes, food is fixed at the bottom or the inner side wall of the box body, and a fixing band is arranged at the joint of the box body and the box cover; the diameter of the air holes is 0.80-1.00 mm. The releaser is small in size and easy to operate, food is dispersedly fixed in the box body and cannot roll back and forth, collision caused by severe shaking in the transportation process can be avoided, the food tiling area is large, ladybug larvae cannot be gathered together for taking and eating, and suicide can be effectively reduced.
Description
Technical Field
The invention relates to the technical field of biological control of pests in agriculture and forestry plant protection, in particular to a ladybug larva releaser and a use method thereof.
Background
At present, the country carries out a green production mode, promotes agricultural sustainable development, and the agricultural ministry puts forward concepts and targets of 'double reduction of pesticide and fertilizer' and 'zero increase of pesticide' for the first time, which puts forward new requirements on the prevention and control mode of agricultural diseases and insect pests, and the biological prevention and control method is bound to become a mainstream mode; beneficial ladybug is released to control aphids, scale insects, whiteflies and other pests of agricultural and forestry crops, and chemical pesticides used for controlling the pests can be effectively reduced or replaced.
The ladybug release mainly comprises egg card release, larva release and adult release modes, and after released, the ladybug eggs are easily affected by the environment temperature and humidity and the attack of ants and other insects to affect the incubation of the ladybug eggs; after the ladybug imagoes are released, the ladybug imagoes can fly and have strong running power and can be diffused and escaped everywhere due to wings; the ladybug larvae can effectively fill the defects of the two insect states in field application, mainly act in climbing, cannot escape quickly, can timely prey on pests after release, and need to prey on a large amount of ladybug larvae for completing growth and development, thereby playing a high-efficiency prevention and control role on the pests in the field.
The existing ladybug larvae are transported and released mostly by large bottles filled with broken paper slips, each bottle of the large bottles with dozens to 100 ladybug larvae, the broken paper slips and the like are taken as separators, and the encounter of the ladybugs can be reduced to a certain extent, so that the killing of the ladybugs is reduced, but the problems that the ladybugs can not timely find supplementary food during transportation, die or self-killing, and can not timely find an outlet during release to achieve the control effect exist, and the field pests are distributed dispersedly, and the large bottle package is not easy to release according to the distribution of the field pests.
Therefore, the technical problem to be solved by the present invention is how to provide a container for controlling food storage and transportation temperature by packaging small containers, fixing food, and accurately dispensing food, and a method for using the same to achieve control effect.
Disclosure of Invention
In view of the above, the invention provides a ladybug larva releaser and a use method thereof, wherein a container for containing ladybug larvae is small in volume, the effective control effect can be achieved by adopting the container to package, fix food, control storage and transportation temperature and accurately throw in, and the container is small in volume and is easy to release according to the distribution of field pests.
In order to achieve the purpose, the invention adopts the following technical scheme: a ladybug larva releaser comprises a box body and a box cover, wherein the box body and the box cover are movably connected;
the side wall of the box body is provided with a plurality of air holes, food is fixed at the bottom or the inner side wall of the box body, and a fixing band is arranged at the joint of the box body and the box cover;
the diameter of the air holes is 0.80-1.00 mm.
The invention has the beneficial effects that: the releaser is small in size and easy to operate, food is dispersedly fixed in the box body and cannot roll back and forth, collision caused by severe shaking in the transportation process can be avoided, the food tiling area is large, ladybug larvae cannot be gathered together for taking and eating, and suicide can be effectively reduced.
Preferably, the box body is cylindrical, the volume of the box body is 25.00mL, the diameter of the top of the box body is 38.00mm, the diameter of the bottom of the box body is 29.00mm, and the height of the box body is 32.00 mm.
Preferably, the food is fixed on the bottom or the inner side wall of the box body in an adhesive mode.
Preferably, the fixing band is a rubber band or an elastic string.
The invention also provides a using method of the ladybug larva releaser, which comprises the following steps:
(1) coating a layer of glue on the bottom or the inner side wall of the box body of the ladybug larva releaser, covering food for the ladybug larvae to eat on the glue, and drying the glue in the box for later use;
(2) putting ladybug larvae with the same age in the box body in the step (1), covering the box cover, and storing in an incubator with the temperature of 6-12 ℃ and the relative humidity of 55-85%;
(3) when aphid spotting occurs, selecting a central plant in which aphids occur, releasing ladybug larvae, and diffusing the ladybug larvae to the periphery;
the specific operation of releasing the ladybug larvae is as follows: selecting a branch in an aphid-generating area, winding a rubber band around the branch, and fixing the branch by sleeving a box with the rubber band; the lid is then opened and ladybug larvae will climb along the edge of the box to the shoots to prey on the aphids.
The invention limits the placing number of ladybug larvae with different day ages and limits the environment in the incubator, so that the activity of the larvae can be reduced, and the self-killing can be reduced.
The ladybug larvae in the invention can keep 6-12 ℃ in the transportation process to ensure lower mortality. Storage conditions are as follows: the box is opened after receiving the goods, ventilated and if it is required to be released the next day, the releaser is kept in an environment of 6-20 ℃ (air conditioned room) and sprayed with water to provide humidity.
Preferably, in the step (2), when the ladybug larvae with the same day age are placed in the box bodies, 5-10 ladybug larvae are placed in each box body with the day age of 1-2, and no more than 5 ladybug larvae are placed in each box body with the day age of 3.
Preferably, in the step (3), when the aphid spot occurs, when the aphid rate of the crops is more than 10% and the occurrence amount of the aphids exceeds 200 per branch, the biological pesticide is firstly adopted for controlling, and the ladybug larvae are released after the aphid rate of the crops is reduced to 4-6%.
Preferably, in the step (3), the quantity ratio of the ladybug to the aphid is 1:40-1: 50; the temperature of the aphid generating area is 15-32 ℃, and the relative humidity is 50-85%.
According to the technical scheme, compared with the prior art, the ladybug larva releaser and the use method thereof are disclosed, the ladybug larva releaser is adopted to release ladybug larvae in a citrus orchard, 600 ladybug larvae are released per mu, the population growth of aphids is effectively controlled, and the control effect reaches 60-70% after 1 month; moreover, the container in the invention has small volume, convenient operation and low cost.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of the structure provided by the present invention;
wherein, 1-box cover, 2-box body, 3-air hole, 4-rubber band, 5-food.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The ladybug larva releaser comprises a box body 2 and a box cover 1, wherein the box body 2 is movably connected with the box cover 1;
the side wall of the box body 2 is provided with 10 air holes 3, food 5 is bonded at the bottom of the box body 2, and a rubber band 4 is arranged at the joint of the box body 2 and the box cover 1;
the diameter of the air holes 3 is 1 mm.
The box body 2 and the box cover 1 are connected into a whole transparent plastic box.
The using method of the ladybug larva releaser comprises the following steps:
(1) coating a layer of glue on the bottom or the inner side wall of the box body of the ladybug larva releaser, covering the killed Mediterranean pink rice borer eggs on the glue, and drying the glue in the box for later use;
(2) carefully placing 3-day-old ladybug larvae into the box body in the step (1), covering the box cover, and storing in an incubator at the temperature of 6 ℃ and the relative humidity of 55%;
(3) when aphid spot-filming occurs, selecting a central plant in which aphids occur, when the aphid rate of crops is more than 10% and the aphid occurrence amount exceeds 200 per branch, firstly adopting biological pesticide to control, and releasing ladybug larvae according to the ratio of 1:40 of ladybug to aphid when the aphid rate of the crops is reduced to 5%, so that the ladybug larvae are diffused to the periphery; the temperature of the aphid-developing area was 25 ℃ and the relative humidity 65%.
The specific operation of releasing the ladybug larvae is as follows: selecting a branch in an aphid-generating area, winding a rubber band around the branch, and fixing the branch by sleeving a box with the rubber band; the lid is then opened and ladybug larvae will climb along the edge of the box to the shoots to prey on the aphids.
Experiment of
Selecting a citrus test field in Fujian city, Fuzhou province, Minhou county, Fujian province, selecting about 10 mu of relatively independent areas which are just damaged by aphids as experimental objects, selecting about 65 plants per mu of land, adopting a 5-point sampling method during investigation, respectively selecting 1 plant at the 4 corners and the middle of the land, respectively taking one branch from the upper, middle and lower parts of each plant in the east, west, south and north directions, and counting the total aphid amount of each plant. 600 harmonia axyridis larvae are released by the method in the example 1, samples are taken every 10 days after the harmonia axyridis larvae are released, the aphid quantity of each tree is counted, the control effect is calculated, and the results are shown in the following table 1.
Control effect (%) - (number of live insects before control-number of live insects after control)/number of live insects before control x 100
Aphid control after releasing harmonia axyridis larvae (%)
Table 1 efficacy data using the method of example 1
As can be seen from the above Table 1, the control effect on aphids in the experimental field reaches 63.91% 30 days after the harmonia axyridis larvae are released.
Example 2
The ladybug larva releaser comprises a box body 2 and a box cover 1, wherein the box body 2 is movably connected with the box cover 1;
the side wall of the box body 2 is provided with 10 air holes 3, food 5 is bonded at the bottom of the box body 2, and a rubber band 4 is arranged at the joint of the box body 2 and the box cover 1;
the diameter of the air holes 3 is 1 mm.
The box body 2 and the box cover 1 are connected into a whole transparent plastic box.
The using method of the ladybug larva releaser comprises the following steps:
(1) coating a layer of glue on the bottom or the inner side wall of the box body of the ladybug larva releaser, covering the killed Mediterranean pink rice borer eggs on the glue, and drying the glue in the box for later use;
(2) carefully placing 3-day-old ladybug larvae into the box body in the step (1), covering the box cover, and storing in an incubator at the temperature of 6 ℃ and the relative humidity of 55%;
(3) when aphid spot-filming occurs, selecting a central plant for generating aphids, when the aphid rate of crops is more than 10% and the aphid generation amount exceeds 200 per branch, firstly adopting biological pesticide to control, and releasing ladybug larvae according to the beneficial and harmful ratio of 1:50 to enable the ladybug larvae to diffuse to the periphery when the aphid rate of the crops is reduced to 5%; the temperature of the aphid-developing area was 25 ℃ and the relative humidity 65%.
The specific operation of releasing the ladybug larvae is as follows: selecting a branch in an aphid-generating area, winding a rubber band around the branch, and fixing the branch by sleeving a box with the rubber band; the lid is then opened and ladybug larvae will climb along the edge of the box to the shoots to prey on the aphids.
In the test field, 5 net chambers (100X 100cm) were provided, in which cowpeas were densely planted, and about 200 cowpeas per net chamber. When the cowpea seedlings grow to the 2-leaf stage and are about 10cm high, 2000 bean aphids are inoculated into each net room, after 10 days, the number of the aphids in each net room and the number of the aphids as harmful plants are counted according to a five-point sampling method and 5 plants per point. Proportionally putting the ladybug larvae, and observing and recording the ladybug larvae once every 5 days.
Table 2 examples 2 aphid population changes after ladybug larvae release
As can be seen from the table 2, after 10 days, the number of aphids of each cowpea seedling in 5 net chambers is respectively 49, 55, 57, 54 and 49, the number of aphid-occurring plants is respectively 121, 152, 133, 154 and 116, 200 larvae are put into each net chamber, after 5 days of releasing ladybug larvae, the number of aphids still shows a growth trend, and decreases from 10 days to 10 days, and the ladybug larvae almost all grow to adults until 20 days, at the moment, the number of aphids is basically reduced to below 10 average plants, but as the number of the initial aphids is large, the cowpea seedlings are seriously damaged, the number of leaf rolls is large, and the plant growth potential is poor. Therefore, besides the beneficial and harmful ratio during prevention and control, the prevention and control time is also important, and ladybug larvae are released in the initial stage of aphids to achieve the best prevention and control effect.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A ladybug larva releaser is characterized by comprising a box body and a box cover, wherein the box body and the box cover are movably connected;
the side wall of the box body is provided with a plurality of air holes, food is fixed at the bottom or the inner side wall of the box body, and a fixing band is arranged at the joint of the box body and the box cover;
the diameter of the air holes is 0.80-1.00 mm.
2. The ladybug larva releaser of claim 1, wherein the box is cylindrical with a volume of 25.00mL, a top diameter of 38.00mm, a bottom diameter of 29.00mm and a height of 32.00 mm.
3. The ladybug larva releaser of claim 1, wherein the food is fixed to the bottom or inner side wall of the box body by means of bonding.
4. The ladybug larva releaser according to claim 1, wherein the fixing strap is a rubber band or a thin rope with elasticity.
5. The using method of the ladybug larva releaser is characterized by comprising the following steps:
(1) coating a layer of glue on the bottom or the inner side wall of the box body of the ladybug larva releaser in any one of claims 1-4, covering food for ladybug larvae on the glue, and drying the glue in the box for later use;
(2) putting ladybug larvae with the same age in the box body in the step (1), covering the box cover, and storing in an incubator with the temperature of 6-12 ℃ and the relative humidity of 55-85%;
(3) when aphid spotting occurs, selecting a central plant in which aphids occur, releasing ladybug larvae, and diffusing the ladybug larvae to the periphery;
the specific operation of releasing the ladybug larvae is as follows: selecting a branch in an aphid-generating area, winding a rubber band around the branch, and fixing the branch by sleeving a box with the rubber band; the lid is then opened and ladybug larvae will climb along the edge of the box to the shoots to prey on the aphids.
6. The use method of the ladybug larva releaser according to claim 5, wherein in the step (2), when ladybug larvae with the same day age are placed in the box bodies, 5-10 ladybug larvae are placed in each box body with 1-2 day age, and no more than 5 ladybug larvae are placed in each box body with 3 day age.
7. The use method of the ladybug larva releaser according to claim 5, wherein in the step (3), when the aphid spot occurs, when the aphid rate of the crops is more than 10% and the aphid occurrence amount exceeds 200 per branch, the biological pesticide is firstly adopted for control, and the ladybug larva is released after the aphid rate of the crops is reduced to 4-6%.
8. The use method of the ladybug larva releaser according to the claim 7, characterized in that in the step (3), the quantity ratio of released ladybug to aphid is 1:40-1: 50; the temperature of the aphid generating area is 15-32 ℃, and the relative humidity is 50-85%.
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| CN202011594857.2A CN112674035A (en) | 2020-12-29 | 2020-12-29 | Ladybug larva releaser and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113749070A (en) * | 2021-10-12 | 2021-12-07 | 邯郸市农业科学院 | Wheat aphid prevention and cure device |
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| CN101836621A (en) * | 2009-12-30 | 2010-09-22 | 吉林农业大学 | Overwintering generation harmonia axyridis collection, low-temperature storage and release method |
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| CN209546652U (en) * | 2019-01-11 | 2019-10-29 | 华南农业大学 | A kind of Predaceous Coccinellids release box for storing transport |
| CN111374095A (en) * | 2020-02-19 | 2020-07-07 | 四川省农业科学院植物保护研究所 | Cultivation method of predatory ladybug |
| CN211211043U (en) * | 2019-08-20 | 2020-08-11 | 云南格瑞生物科技有限公司 | Insect scattering box |
| CN211932144U (en) * | 2019-05-29 | 2020-11-17 | 溧阳益植生物技术有限公司 | Package release bag of ladybug larva |
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2020
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101836621A (en) * | 2009-12-30 | 2010-09-22 | 吉林农业大学 | Overwintering generation harmonia axyridis collection, low-temperature storage and release method |
| CN203207043U (en) * | 2013-05-08 | 2013-09-25 | 云南瑞升烟草技术（集团）有限公司 | Larval ladybird transportation release device |
| CN103478117A (en) * | 2013-08-21 | 2014-01-01 | 云南瑞升烟草技术（集团）有限公司 | Diffusion breeding and releasing method for preventing myzus persicae through harmonia axyridis |
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| CN209546652U (en) * | 2019-01-11 | 2019-10-29 | 华南农业大学 | A kind of Predaceous Coccinellids release box for storing transport |
| CN211932144U (en) * | 2019-05-29 | 2020-11-17 | 溧阳益植生物技术有限公司 | Package release bag of ladybug larva |
| CN211211043U (en) * | 2019-08-20 | 2020-08-11 | 云南格瑞生物科技有限公司 | Insect scattering box |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113749070A (en) * | 2021-10-12 | 2021-12-07 | 邯郸市农业科学院 | Wheat aphid prevention and cure device |
| CN113749070B (en) * | 2021-10-12 | 2022-08-16 | 邯郸市农业科学院 | Wheat aphid prevention and cure device |
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Application publication date: 20210420 |