CN112655961A - Composition for relieving physical fatigue - Google Patents
Composition for relieving physical fatigue Download PDFInfo
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Abstract
The invention relates to the technical field of health-care food, and particularly discloses a composition for relieving physical fatigue and a preparation method thereof. The composition for relieving physical fatigue comprises the following components in parts by weight: 5-45 parts of American ginseng extract, 5-45 parts of epimedium extract, 1-35 parts of rhodiola rosea extract and 1-30 parts of saussurea involucrata culture. The composition for relieving physical fatigue provided by the invention adopts the medicine characteristics of tonifying five internal organs and nourishing heart, lung and kidney, and is assisted with the regulation of central nervous system, the improvement of the excitation and inhibition process of brain, the enhancement of the immunity of the organism, the regulation of the internal environment of human body from multiple aspects, the improvement of blood circulation, the increase of oxygen carrying capacity of cells and the effective improvement of the anti-fatigue effect. The formula regulates the internal environment of a human body as a whole, assists middle-warmer energy, coordinates viscera and meridian functions and further corrects the preponderance and decline of yin and yang on the premise of supplementing essential nutrients such as vitamins, minerals, proteins and the like required by the body, so that the body is restored to a normal state of yin balancing and yang secret.
Description
Technical Field
The invention relates to the technical field of health-care food, in particular to a composition for relieving physical fatigue and a preparation method thereof.
Background
With the acceleration of modern life rhythm and the increase of working pressure, the incidence of fatigue in people is higher and higher, and the life quality and the working efficiency of people are seriously influenced. Chronic Fatigue Syndrome (CFS) is an intermediate state between health and disease, is a functional disorder, does not have organic lesions, but is gradually accumulated if not corrected in time, finally causes the regulation of nervous, endocrine and immune systems of the body to be abnormal, even presents organic lesions, and becomes an important factor for destroying physical and mental health of human beings. Due to the fact that the work task of the modern society is heavy, workers in all levels have chronic fatigue syndromes of different degrees, although people in a fatigue state can continue to work and study, the work, study capacity and efficiency are obviously reduced, and fatigue symptoms cannot be relieved due to rest. Therefore, fatigue is considered by the national centers for disease control and prevention in the united states as the "largest enemy of mankind in the 21 st century".
Traditional Chinese medicine has unique advantages in the aspects of eliminating fatigue and enhancing athletic ability. It is proved that Chinese traditional medicine and food therapy medicine not only can increase energy substances in human body, but also has the functions of regulating neuroendocrine system, strengthening metabolism, stimulating secretion and release of hormone in body, enhancing functions of cardiovascular system, digestive system, hematopoietic system, skeletal system and the like, and delaying and eliminating the generation of over fatigue. At present, although the existing health-care foods for relieving physical fatigue are various in types, the products generally have the defects of complex components and unobvious effect, and meanwhile, the health-care foods are expensive due to complex components, cannot be taken for a long time, and cannot achieve the effect of continuously and systematically relieving physical fatigue. Therefore, the development of the health food for relieving physical fatigue, which has simple and effective components and can be taken for a long time, has very important significance.
Disclosure of Invention
Aiming at the technical problems that the health-care food for relieving physical fatigue in the prior art has complex components and unobvious anti-fatigue effect, the invention provides a composition for relieving physical fatigue.
The invention also provides a preparation method of the composition for relieving physical fatigue.
The invention also provides a health food for relieving physical fatigue.
The invention also provides a tablet for relieving physical fatigue.
The invention also provides a preparation method of the tablet for relieving physical fatigue.
In order to achieve the above purpose, the embodiment of the invention adopts the following technical scheme:
the composition for relieving physical fatigue comprises the following components in parts by weight: 5-45 parts of American ginseng extract, 5-45 parts of epimedium extract, 1-35 parts of rhodiola rosea extract and 1-30 parts of saussurea involucrata culture.
Compared with the prior art, the invention selects the American ginseng, the epimedium herb, the rhodiola rosea and the saussurea involucrata culture to form an organic compound according to the main causes of fatigue formation, adopts the medicine characteristics of tonifying five internal organs and nourishing heart, lung and kidney, and regulates the central nervous system, improves the excitation and inhibition process of brain, enhances the immunity of organisms, regulates the internal environment of human body from multiple aspects, and achieves the effects of improving blood circulation, increasing oxygen carrying capacity of cells and effectively improving fatigue resistance. The formula regulates the internal environment of a human body as a whole, strengthens the body resistance to eliminate pathogenic factors, strengthens middle-jiao energy, coordinates the functions of viscera and meridians and collaterals, and further corrects the preponderance and decline of yin and yang on the premise of supplementing essential nutrients such as vitamins, minerals, proteins and the like required by the body, so that the body is recovered to a normal state of yin balance and yang secret. The product of the invention does not use any food preservative, is safe and reliable, and is suitable for long-term eating.
The American ginseng is cool in nature, can be used for tonifying heart, lung and kidney, has multiple effects of tonifying lung, lowering fire, producing body fluid, relieving restlessness, and the like, has the main active components of American ginseng saponin, can effectively enhance the central nervous function, achieves the effects of eliminating fatigue, enhancing memory and the like, can strengthen cardiac muscle and enhance the activity of heart, and can promote serum protein synthesis, bone marrow protein synthesis, organ protein synthesis and the like, improve the immunity of the organism, enhance the physical strength, and effectively delay the generation of fatigue.
The epimedium herb is pungent and sweet in taste and warm in nature, walks liver and kidney meridians, contains icariin, polysaccharide, volatile oil, ceryl alcohol, phytosterol, tannin, vitamin E and other components, and the icariin can increase cardiovascular and cerebrovascular blood flow, promote hematopoiesis, increase cerebral blood flow and improve microcirculation, has obvious improvement effect on cerebral ischemia and hypoxia, and can effectively relieve brain fatigue; the epimedium polysaccharide has the function of promoting the immune function of the organism, can increase the number of white blood cells and lymphocytes and improve the immune function of the organism; the epimedium also contains a large amount of flavone, and the epimedium total flavone has the function of delaying senility; in addition, herba Epimedii is rich in trace elements such as Zn, Mn, Fe, etc., and alkaloid, and has effects of regulating neuroendocrine immunity. The American ginseng polysaccharide contained in the American ginseng can improve the biological activity of epimedium flavone, obviously enhance the anti-aging effect of epimedium and further obviously delay the generation of fatigue.
Rhodiola rosea is cold in nature and contains flavonoids, saponins, phenols, coumarins, enzymes, volatile oils and the like, and in addition, the rhodiola rosea also contains 18 amino acids, 35 trace elements, vitamin A, C, D, E, B and the like. Salidroside can accelerate blood circulation by regulating capillary vasoconstriction, and improve adaptability to exercise anoxic environment; the superoxide dismutase contained in the rhodiola rosea can improve the ATP content of the organism, reduce the formation of lactic acid and acetone, increase the creatine phosphokinase content and improve the total protein content of serum, thereby obviously improving the fatigue formed by a large amount of sports; the B vitamins can help protein, fat and sugar to be metabolized and utilized, provide energy for brain and body, and relieve fatigue; in addition, the rhodiola rosea contains various amino acids, vitamins and rich elements such as calcium, iron, zinc and the like, can improve physiological and biochemical changes of the brain nerve synapse part, and provides multi-aspect function coordination for improving the fatigue state of the organism. The rich zinc element contained in the epimedium herb and the superoxide dismutase in the rhodiola rosea are cooperated, so that the activity of middle skeletal muscle Lactate Dehydrogenase (LDH) after exercise can be obviously improved, the LDH release is promoted, the decomposition of lactic acid and urea nitrogen is accelerated, and in case of overstrain, the utilization rate of tissue cells to energy can be improved, and the elimination of fatigue is accelerated.
The saussurea involucrate culture has great heat of nature, enters three meridians of liver, spleen and kidney, can tonify yin and yang, nourish nerves, contains rich active substances such as flavonoid, polysaccharide, chlorogenic acid, syringin, alkaloid and the like, also contains 18 amino acids, not only contains active ingredients similar to natural saussurea involucrate, but also has pharmacological activity in various aspects equivalent to the active ingredients. Polysaccharide in the saussurea involucrata culture has obvious scavenging effect on superoxide anion free radicals in organisms, and can delay the generation of fatigue; the total flavone in the culture of saussurea involucrate has the function of immunoregulation and can enhance the immunity of the organism; the saussurea involucrate culture also contains unique flavones and saussurea involucrate lactone, can effectively remove oxygen free radicals, prevents chromosome distortion, and has excellent radiation protection capability; the syringin contained in the saussurea involucrate can promote the body to absorb and utilize active components in the epimedium, the American ginseng and the rhodiola rosea, effectively reduce the generation of blood urea nitrogen and lactic acid, improve the capability of the body to bear exercise load, delay the generation of fatigue, accelerate the elimination of fatigue, further improve the effects of delaying the appearance of fatigue and accelerating the elimination of fatigue of the three components, and the abundant vitamin E contained in the three components, phenols contained in the rhodiola rosea, tannin contained in the epimedium and the like can enhance the stability of the syringin in a human body, further ensure the exertion of the activity enhancement effect of the syringin on the three components.
The saussurea involucrate is a high-heat substance and is not suitable for people with yin deficiency and fire excess to take for a long time, and the rhodiola rosea and the American ginseng with specific content can regulate the heat of the saussurea involucrate, so that the formula can be supplemented without dryness, and the saussurea involucrate is suitable for various people to take for a long time. The American ginseng, the rhodiola rosea and the epimedium can strengthen the efficacy of tonifying qi and tonifying deficiency of the saussurea involucrata culture, and the combination of the four medicines can tonify the liver, heart, spleen, lung and kidney five internal organs, can tonify qi and replenish vital essence and relieve physical fatigue.
Preferably, the composition comprises the following components in parts by weight: 20-30 parts of American ginseng extract, 20-30 parts of epimedium extract, 10-20 parts of rhodiola rosea extract and 2-10 parts of saussurea involucrata culture.
Preferably, the composition comprises the following components in parts by weight: 25 parts of American ginseng extract, 25 parts of epimedium extract, 15 parts of rhodiola rosea extract and 5 parts of saussurea involucrata culture.
Preferably, the preparation method of the American ginseng extract and the rhodiola rosea extract comprises the following steps:
pulverizing radix Panacis Quinquefolii and radix Rhodiolae to obtain radix Panacis Quinquefolii coarse powder and radix Rhodiolae coarse powder, respectively adding 7-9 times of ethanol solution with mass concentration of 65-75% into the radix Panacis Quinquefolii coarse powder and radix Rhodiolae coarse powder, heating and reflux extracting for 1.5-2.5h, and filtering to obtain first residue and first filtrate; adding 5-6 times of 65-75% ethanol solution into the first filter residue, heating and refluxing for extraction for 1.5-2.5h, and filtering to obtain a second filter residue and a second filtrate; adding 5-6 times of 65-75% ethanol solution into the second filter residue, heating and reflux-extracting for 1-2 hr, filtering to obtain a third filtrate, mixing the first filtrate, the second filtrate and the third filtrate, and spray-drying to obtain the American ginseng extract and the rhodiola rosea extract respectively.
Preferably, the preparation method of the epimedium extract comprises the following steps: adding 65-75% ethanol solution 9-11 times the weight of herba Epimedii, heating and reflux extracting for 2-3 times (each time for 2-3 hr), filtering to obtain filtrate, eluting the filtrate with chromatographic column filled with macroporous adsorbent resin, collecting eluate, concentrating by evaporation, and spray drying to obtain herba Epimedii extract.
The American ginseng, the epimedium herb and the rhodiola root contain various effective components, such as flavone, total saponin and the like, have obvious effects of resisting fatigue and exciting nerve centers, and active substances in the American ginseng, the rhodiola root and the epimedium can be fully extracted by adopting the preparation method, so that the anti-fatigue effect of the composition is improved.
Preferably, in the second step, the model of the macroporous adsorption resin is D101, and the elution solvent is 60-80% ethanol solution.
The refining method can remove a large amount of impurities in the extract, and ensure that the extract mainly contains active substances such as icariin, flavone and the like, thereby improving the anti-fatigue effect of the extract.
The invention also provides a preparation method of the composition for relieving physical fatigue, which at least comprises the following steps:
weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract and the saussurea involucrata culture according to the design proportion, and uniformly mixing to obtain the composition for relieving physical fatigue.
The invention also provides a health food for relieving physical fatigue, which comprises the composition for relieving physical fatigue.
After the composition for relieving physical fatigue is prepared into health-care food, the composition is more suitable for being taken and carried by eaters, is convenient to eat for a long time, and achieves the effect of relieving physical fatigue.
Preferably, the formulation of the health food for relieving physical fatigue is powder, granules, capsules or tablets.
When the composition for relieving physical fatigue is prepared into health-care food, the composition comprises effective pharmaceutical ingredients and pharmaceutically acceptable carriers such as excipient and auxiliary materials. These carriers function to facilitate the processing of the active ingredients into preparations and to carry the active ingredients to the site of action in the body.
The invention also provides a physical fatigue relieving tablet, which comprises the composition for relieving physical fatigue, and also comprises the following raw materials in parts by weight: 10-40 parts of microcrystalline cellulose, 1-20 parts of carboxymethyl starch sodium and 1-2 parts of magnesium stearate.
The invention also provides a preparation method of the physical fatigue relieving tablet, which at least comprises the following steps:
step a, weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract, the snow lotus culture and the microcrystalline cellulose according to a design ratio, respectively sieving and uniformly mixing to obtain a premixed material;
b, sieving the premixed material with a 14-16-mesh sieve for granulation, drying, sieving with a 14-16-mesh sieve for granulation, and obtaining granules;
and c, adding the carboxymethyl starch sodium and the magnesium stearate into the granules, uniformly mixing to obtain total mixed granules, and tabletting the total mixed granules to obtain the physical fatigue relieving tablets.
The preparation method has the advantages that the obtained product has good stability and is convenient to eat through mixing, granulating and tabletting, the preparation process has no complex procedures, and the method is simple and is suitable for industrial production.
Preferably, the preparation method of the tablet for relieving physical fatigue further comprises the following steps:
and d, dissolving the film coating premix with purified water to prepare coating liquid with the solid content of 18-22%, and coating the physical fatigue relieving tablets until the weight of the plain tablets is increased by 1.8-2.2%.
Tablets containing the composition for relieving physical fatigue can also be coated to provide a dosage form for achieving the effect of prolonging the drug effect.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The embodiment of the invention provides a physical fatigue relieving tablet which comprises the following components in parts by weight:
5 parts of American ginseng extract, 45 parts of epimedium extract, 1 part of rhodiola rosea extract, 30 parts of saussurea involucrate culture, 40 parts of microcrystalline cellulose, 1 part of carboxymethyl starch sodium and 1 part of magnesium stearate.
The preparation steps of the tablet for relieving physical fatigue are as follows:
step one, grinding American ginseng and rhodiola rosea roots to obtain American ginseng coarse powder and rhodiola rosea coarse powder, respectively adding ethanol solution with the mass concentration of 70% and the mass of 8 times of that of the American ginseng coarse powder and the rhodiola rosea coarse powder, heating, refluxing and extracting for 2.5 hours, and filtering to obtain first filter residue and first filtrate; adding 5.5 times of 70% ethanol solution into the first filter residue, heating and refluxing for extraction for 2.5h, and filtering to obtain a second filter residue and a second filtrate; adding 5.5 times of 70% ethanol solution into the second filter residue, heating and refluxing for 1 hour, filtering to obtain a third filtrate, mixing the first filtrate, the second filtrate and the third filtrate, and spray drying to obtain an American ginseng extract and a rhodiola rosea extract respectively;
step two, adding an ethanol solution with the mass concentration of 65 percent, the mass of which is 11 times that of the epimedium herb, into the epimedium herb, heating, refluxing and extracting for 3 times, wherein the extraction time is 2 hours each time, filtering to obtain a filtrate, passing the filtrate through a chromatographic column filled with macroporous adsorption resin (D101), eluting with an 80 percent ethanol solution, collecting an eluent, evaporating, concentrating and spray-drying to obtain an epimedium herb extract;
weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract, the snow lotus culture and the microcrystalline cellulose according to the design ratio, respectively sieving by a 60-mesh sieve, and uniformly mixing to obtain a premixed material;
step four, sieving the premixed material with a 16-mesh sieve for granulation, drying, sieving with the 16-mesh sieve for size stabilization, and obtaining granules;
and fifthly, adding the carboxymethyl starch sodium and the magnesium stearate into the granules, uniformly mixing to obtain total mixed granules, and tabletting the total mixed granules to obtain the physical fatigue relieving tablets.
The tablets prepared in this example weighed 0.55g and were taken 2 tablets at a time, once a day.
Example 2
The embodiment of the invention provides a physical fatigue relieving tablet which comprises the following components in parts by weight:
45 parts of American ginseng extract, 5 parts of epimedium extract, 35 parts of rhodiola rosea extract, 1 part of saussurea involucrate culture, 10 parts of microcrystalline cellulose, 20 parts of carboxymethyl starch sodium and 2 parts of magnesium stearate.
The preparation steps of the tablet for relieving physical fatigue are as follows:
step one, grinding American ginseng and rhodiola rosea roots to obtain American ginseng coarse powder and rhodiola rosea coarse powder, respectively adding ethanol solution with the mass concentration of 65% and the mass of 7 times of that of the American ginseng coarse powder and the rhodiola rosea coarse powder, heating, refluxing and extracting for 2.5 hours, and filtering to obtain first filter residue and first filtrate; adding 5 times of 65% ethanol solution into the first filter residue, heating and refluxing for extraction for 1.5h, and filtering to obtain a second filter residue and a second filtrate; adding 5 times of 65% ethanol solution into the second filter residue, heating and refluxing for 2 hours, filtering to obtain a third filtrate, combining the first filtrate, the second filtrate and the third filtrate, and performing spray drying to respectively obtain an American ginseng extract and a rhodiola rosea extract;
step two, adding 75% ethanol solution with the mass concentration 9 times of the mass of the epimedium herb, heating and refluxing for 2 times, wherein the extraction time is 3 hours each time, filtering to obtain filtrate, passing the filtrate through a chromatographic column filled with macroporous adsorption resin (D101), eluting with 60% ethanol solution, collecting eluent, evaporating and concentrating, and spray drying to obtain an epimedium herb extract;
weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract, the snow lotus culture and the microcrystalline cellulose according to the design ratio, respectively sieving by a 60-mesh sieve, and uniformly mixing to obtain a premixed material;
step four, sieving the premixed material with a 14-mesh sieve for granulation, drying, sieving with the 14-mesh sieve for size stabilization, and obtaining granules;
and fifthly, adding the carboxymethyl starch sodium and the magnesium stearate into the granules, uniformly mixing to obtain total mixed granules, and tabletting the total mixed granules to obtain the physical fatigue relieving tablets.
The tablets prepared in this example weighed 0.55g and were taken 2 tablets at a time, once a day.
Example 3
The embodiment of the invention provides a physical fatigue relieving tablet which comprises the following components in parts by weight:
25 parts of American ginseng extract, 25 parts of epimedium extract, 15 parts of rhodiola rosea extract, 5 parts of saussurea involucrate culture, 20 parts of microcrystalline cellulose, 10 parts of carboxymethyl starch sodium, 1.5 parts of magnesium stearate and 2 parts of film coating premix.
The preparation steps of the tablet for relieving physical fatigue are as follows:
step one, grinding American ginseng and rhodiola rosea roots to obtain American ginseng coarse powder and rhodiola rosea coarse powder, respectively adding ethanol solution with the mass concentration of 75% and the mass of 9 times of that of the American ginseng coarse powder and the rhodiola rosea coarse powder, heating, refluxing and extracting for 2 hours, and filtering to obtain first filter residue and first filtrate; adding 6 times of 75% ethanol solution into the first filter residue, heating, refluxing and extracting for 2h, and filtering to obtain a second filter residue and a second filtrate; adding 6 times of 75% ethanol solution into the second filter residue, heating and refluxing for extraction for 1.5 hours, filtering to obtain a third filtrate, combining the first filtrate, the second filtrate and the third filtrate, and performing spray drying to respectively obtain an American ginseng extract and a rhodiola rosea extract;
step two, adding ethanol solution with the mass concentration of 70% which is 10 times of the mass of the epimedium herb into the epimedium herb, heating and refluxing for extraction for 3 times, wherein the extraction time is 2.5h each time, filtering to obtain filtrate, passing the filtrate through a chromatographic column filled with macroporous adsorption resin (D101), eluting with 70% ethanol solution, collecting eluent, evaporating and concentrating, and performing spray drying to obtain an epimedium herb extract;
weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract, the snow lotus culture and the microcrystalline cellulose according to the design ratio, respectively sieving by a 60-mesh sieve, and uniformly mixing to obtain a premixed material;
step four, sieving the premixed material with a 16-mesh sieve for granulation, drying, sieving with the 16-mesh sieve for size stabilization, and obtaining granules;
step five, adding the carboxymethyl starch sodium and the magnesium stearate into the granules, uniformly mixing to obtain total mixed granules, and tabletting the total mixed granules to obtain plain tablets;
step six: dissolving the film coating premix with purified water to prepare coating liquid with the solid content of 20%, coating the plain tablets until the weight of the plain tablets is increased by 2%, and thus obtaining the tablets for relieving physical fatigue.
The tablets prepared in this example weighed 0.55g and were taken 2 tablets at a time, once a day.
The composition for relieving physical fatigue can also be prepared into granules and capsules by selecting conventional auxiliary materials in the field of health care products according to a conventional process.
Physical fatigue relieving function test
Test subjects:
the test object is 190 male Kunming mice, the weight of the test object is 18-22g, and the test object is SPF grade.
Dose design:
the physical fatigue relieving tablet prepared in example 3 was designed into three dosage groups of 0.55g/kg.bw, 0.37g/kg.bw and 0.18g/kg.bw, the dosages of the three dosage groups were respectively equal to 30 times, 20 times and 10 times of the recommended dosage of human body, and a negative control group (distilled water) was designed.
Sample preparation: 0.55g, 0.37g and 0.18g of the tablets for relieving physical fatigue prepared in the embodiment 3 are respectively weighed, distilled water is respectively added to 20mL, and the mixture is uniformly mixed for later use.
The test method comprises the following steps:
after pre-swimming, 120 male mice with correct postures are selected and divided into 3 test groups, 40 mice are used in each group and randomly divided into 4 groups, each test group is provided with three dose groups (1 medium dose group, 1 high dose group and 1 low dose group) and 1 negative control group, and each group is provided with 10 mice. One group of tests is a load swimming test, two groups of tests are serum urea measurement tests, three groups of tests are liver glycogen measurement tests, the test sample is orally administered once a day according to the dosage, a negative control group is administered with distilled water with equal volume, the intragastric perfusion volume is 20mL/Kg.
Test methods and results:
1. weight bearing swimming test
After the test object is given for 30min at the last time, the mouse is placed in a swimming box for swimming, the water depth is about 35cm, the water temperature is 25 soil and 1 ℃, and the tail root of the rat is loaded with 5 percent of weight of lead skin. The time from the start of swimming to death of the mouse was recorded as the mouse swimming time (min). The death judgment criteria were: the mouse sinks to the bottom of the swimming tank and stops breathing. And the body weight of the mice was measured before the start of the test and after the end of the test. The results are shown in Table 1.
TABLE 1
| Group of | Initial weight (g) | Final weight (g) | Weight gain (g) | Swimming time (min) | P value |
| High dose group | 20.3±1.1 | 41.6±2.2 | 21.3±1.6 | 34.8±6.36** | 0.000 |
| Middle dose group | 20.5±1.1 | 40.1±2.9 | 19.6±2.3 | 27.10±7.52 | 0.057 |
| Low dose group | 20.6±1.0 | 41.0±2.7 | 20.4±2.3 | 22.10±5.67 | 0.862 |
| Negative control group | 20.6±1.2 | 40.2±2.8 | 19.6±2.0 | 20.30±5.72 | - |
Note: compared with the negative control group, the ratio of the positive control group,**represents P<0.01。
As can be seen from the above results, the weight and weight gain of mice in each period of each dose group are not significantly different from those of the negative control group (P > 0.05); the average swimming time of mice in each dose group is longer than that of the negative control group, and the high dose group has a very significant difference (P < 0.01) compared with the negative control group. The result shows that the sample has the function of prolonging the weight swimming time of the mouse.
2. Serum urea content determination
After 30min of the last administration, the mice swim in water with the temperature of 30 ℃ for 90min without load, after 60min of rest, the eyeballs are pulled out for blood sampling of 0.5mL, and after serum is separated, the serum urea is measured by a full-automatic biochemical analyzer. And the body weight of the mice was measured before the start of the test and after the end of the test. The results are shown in Table 2.
TABLE 2
| Group of | Initial weight (g) | Final weight (g) | Weight gain (g) | Serum urea (mmol/L) | P value |
| High dose group | 20.4±1.1 | 39.8±2.1 | 19.4±2.0 | 8.75±0.93** | 0.000 |
| Middle dose group | 20.3±1.0 | 40.9±2.6 | 20.6±1.8 | 12.08±1.81 | 0.142 |
| Low dose group | 20.2±1.4 | 39.2±3.5 | 19.0±2.4 | 12.74±2.08 | 0.592 |
| Negative control group | 20.3±1.2 | 40.7±2.0 | 20.4±1.3 | 13.51±1.50 | - |
Note: compared with the negative control group, the ratio of the positive control group,**represents P<0.01。
As can be seen from the above results, the weight and weight gain of mice in each period of each dose group are not significantly different from those of the negative control group (P > 0.05); compared with a control group with negative urea content in serum of mice in a high-dose group, the control group has very significant difference (P < 0.01). The results show that the sample has the effect of reducing the urea production of the fatigue mice.
3. Determination of liver glycogen
After 30min of the last administration, the mice were sacrificed, the livers were dissected and removed, and the liver glycogen content was determined according to the kit requirements. And the body weight of the mice was measured before the start of the test and after the end of the test. The results are shown in Table 3.
TABLE 3
| Group of | Initial weight (g) | Final weight (g) | Weight gain (g) | Hepatic glycogen (mg/100 g liver) | P value |
| High dose group | 20.4±1.1 | 40.6±2.0 | 20.2±1.4 | 2348.07±269.27** | 0.003 |
| Middle dose group | 20.7±1.1 | 40.0±2.2 | 19.4±1.7 | 2323.60±344.22** | 0.004 |
| Low dose group | 20.2±1.0 | 39.4±2.0 | 19.2±1.9 | 1985.11±329.12 | 0.385 |
| Negative control group | 20.4±1.0 | 40.2±2.5 | 19.8±2.1 | 1760.66±479.65 | - |
Note: compared with the negative control group, the ratio of the positive control group,**represents P<0.01。
As can be seen from the above results, the weight and weight gain of mice in each period of each dose group are not significantly different from those of the negative control group (P > 0.05); the liver glycogen of mice in each dose group is higher than that of the negative control group, and compared with the negative control group with the liver glycogen content of the mice in the high and medium dose groups, the liver glycogen content of the mice in each dose group has a very significant difference (P < 0.01). The result shows that the sample can improve the liver glycogen storage quantity of the mouse, improve the endurance capacity of the mouse and relieve the physical fatigue.
Example 4
The embodiment of the invention provides a physical fatigue relieving tablet which comprises the following components in parts by weight:
20 parts of American ginseng extract, 30 parts of epimedium extract, 10 parts of rhodiola rosea extract, 10 parts of saussurea involucrate culture, 10 parts of microcrystalline cellulose, 15 parts of carboxymethyl starch sodium, 2 parts of magnesium stearate and 2 parts of film coating premix.
The preparation method of the tablet for relieving physical fatigue is the same as that of the example 3, and the details are not repeated.
Example 5
The embodiment of the invention provides a physical fatigue relieving tablet which comprises the following components in parts by weight:
30 parts of American ginseng extract, 20 parts of epimedium extract, 20 parts of rhodiola rosea extract, 2 parts of saussurea involucrate culture, 30 parts of microcrystalline cellulose, 3 parts of carboxymethyl starch sodium, 1 part of magnesium stearate and 2 parts of film coating premix.
The preparation method of the tablet for relieving physical fatigue is the same as that of the example 3, and the details are not repeated.
Comparative example 1
The comparative example provides a physical fatigue relieving tablet which comprises the following raw materials in parts by weight: 25 parts of American ginseng extract, 25 parts of epimedium extract, 15 parts of rhodiola rosea extract, 5 parts of cervus elaphus linnaeus, 20 parts of microcrystalline cellulose, 10 parts of carboxymethyl starch sodium, 1.5 parts of magnesium stearate and 2 parts of film coating premix.
Comparative example 2
The comparative example provides a physical fatigue relieving tablet which comprises the following raw materials in parts by weight: 25 parts of American ginseng extract, 25 parts of epimedium extract, 10 parts of medlar, 15 parts of rhodiola rosea extract, 5 parts of saussurea involucrate culture, 20 parts of microcrystalline cellulose, 10 parts of carboxymethyl starch sodium, 1.5 parts of magnesium stearate and 2 parts of film coating premix.
Mice of the physical fatigue-alleviating tablets of examples 1 to 5 and comparative examples 1 to 2 were tested for swimming time under load, serum urea nitrogen and liver glycogen according to the test method of the physical fatigue-alleviating test of example 3 above, according to the test method of the high-dose group. The test results of examples 1 to 5, negative control group, and comparative examples 1 to 2 were summarized as shown in Table 4.
TABLE 4
| Group of | Swimming time (min) | Serum urea content (mmol/L) | Hepatic glycogen (mg/100 g liver) |
| EXAMPLE 1 group | 29.08±5.39 | 11.67±1.33 | 2048.61±415.72 |
| EXAMPLE 2 group | 26.73±4.95 | 12.08±1.41 | 1893.54±250.43 |
| EXAMPLE 3 group | 34.80±6.36 | 8.75±0.93 | 2348.07±269.27 |
| EXAMPLE 4 group | 32.93±5.04 | 10.35±2.16 | 2323.60±344.22 |
| EXAMPLE 5 group | 31.56±7.52 | 10.04±1.27 | 2194.27±329.12 |
| Comparative example 1 group | 25.91±5.68 | 12.61±0.81 | 1912.34±287.12 |
| Comparative example 2 group | 27.63±6.12 | 11.73±1.52 | 2018.25±324.12 |
| Negative control group | 20.30±5.72 | 13.51±1.50 | 1760.66±479.65 |
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. The composition for relieving physical fatigue is characterized by comprising the following components in parts by weight: 5-45 parts of American ginseng extract, 5-45 parts of epimedium extract, 1-35 parts of rhodiola rosea extract and 1-30 parts of saussurea involucrata culture.
2. The composition for relieving physical fatigue of claim 1, wherein the composition comprises the following components in parts by weight: 20-30 parts of American ginseng extract, 20-30 parts of epimedium extract, 10-20 parts of rhodiola rosea extract and 2-10 parts of saussurea involucrata culture.
3. The composition for relieving physical fatigue of claim 1, wherein the composition comprises the following components in parts by weight: 25 parts of American ginseng extract, 25 parts of epimedium extract, 15 parts of rhodiola rosea extract and 5 parts of saussurea involucrata culture.
4. The composition for relieving physical fatigue of any one of claims 1-3, wherein the American ginseng extract and the rhodiola rosea extract are prepared by the following steps:
pulverizing radix Panacis Quinquefolii and radix Rhodiolae to obtain radix Panacis Quinquefolii coarse powder and radix Rhodiolae coarse powder, respectively adding 7-9 times of ethanol solution with mass concentration of 65-75% into the radix Panacis Quinquefolii coarse powder and radix Rhodiolae coarse powder, heating and reflux extracting for 1.5-2.5h, and filtering to obtain first residue and first filtrate; adding 5-6 times of 65-75% ethanol solution into the first filter residue, heating and refluxing for extraction for 1.5-2.5h, and filtering to obtain a second filter residue and a second filtrate; adding 5-6 times of 65-75% ethanol solution into the second filter residue, heating and reflux-extracting for 1-2 hr, filtering to obtain a third filtrate, mixing the first filtrate, the second filtrate and the third filtrate, and spray-drying to obtain the American ginseng extract and the rhodiola rosea extract respectively.
5. The composition for relieving physical fatigue as claimed in any one of claims 1 to 3, wherein the epimedium extract is prepared by the following steps: adding 65-75% ethanol solution 9-11 times the weight of herba Epimedii, heating and reflux extracting for 2-3 times (each time for 2-3 hr), filtering to obtain filtrate, eluting the filtrate with chromatographic column filled with macroporous adsorbent resin, collecting eluate, concentrating by evaporation, and spray drying to obtain herba Epimedii extract.
6. A health food for relieving physical fatigue, which comprises the composition for relieving physical fatigue according to any one of claims 1 to 5.
7. The health food for relieving physical fatigue of claim 6, wherein: the dosage form is powder, granule, capsule or tablet.
8. The physical fatigue relieving tablet is characterized by comprising the composition for relieving physical fatigue, which is disclosed by any one of claims 1-5, and further comprising the following raw materials in parts by weight: 10-40 parts of microcrystalline cellulose, 1-20 parts of carboxymethyl starch sodium and 1-2 parts of magnesium stearate.
9. The method for preparing physical fatigue relieving tablets according to claim 8, characterized by at least comprising the following steps:
step a, weighing the American ginseng extract, the rhodiola rosea extract, the epimedium extract, the snow lotus culture and the microcrystalline cellulose according to a design ratio, respectively sieving and uniformly mixing to obtain a premixed material;
b, sieving the premixed material with a 14-16-mesh sieve for granulation, drying, sieving with a 14-16-mesh sieve for granulation, and obtaining granules;
and c, adding the carboxymethyl starch sodium and the magnesium stearate into the granules, uniformly mixing to obtain total mixed granules, and tabletting the total mixed granules to obtain the physical fatigue relieving tablets.
10. The method of preparing a physical fatigue relieving tablet according to claim 9, further comprising the steps of:
and d, dissolving the film coating premix with purified water to prepare coating liquid with the solid content of 18-22%, and coating the physical fatigue relieving tablets until the weight of the plain tablets is increased by 1.8-2.2%.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095304A (en) * | 2017-07-12 | 2017-08-29 | 延边韩工坊健康制品有限公司 | One kind alleviates physical fatigue health food and preparation method thereof |
| CN109077306A (en) * | 2018-08-27 | 2018-12-25 | 辽宁众源生物科技有限公司 | A kind of composition for relieving fatigue and preparation method thereof |
| CN110652006A (en) * | 2019-10-12 | 2020-01-07 | 神威药业集团有限公司 | Composition for relieving physical fatigue and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095304A (en) * | 2017-07-12 | 2017-08-29 | 延边韩工坊健康制品有限公司 | One kind alleviates physical fatigue health food and preparation method thereof |
| CN109077306A (en) * | 2018-08-27 | 2018-12-25 | 辽宁众源生物科技有限公司 | A kind of composition for relieving fatigue and preparation method thereof |
| CN110652006A (en) * | 2019-10-12 | 2020-01-07 | 神威药业集团有限公司 | Composition for relieving physical fatigue and preparation method thereof |
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