CN104857180B - Composition for resisting fatigue and improving immunity and preparation method and application thereof - Google Patents
Composition for resisting fatigue and improving immunity and preparation method and application thereof Download PDFInfo
- Publication number
- CN104857180B CN104857180B CN201510286099.0A CN201510286099A CN104857180B CN 104857180 B CN104857180 B CN 104857180B CN 201510286099 A CN201510286099 A CN 201510286099A CN 104857180 B CN104857180 B CN 104857180B
- Authority
- CN
- China
- Prior art keywords
- composition
- fatigue
- coenzyme
- grape seed
- seed extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a composition for resisting fatigue and improving immunity and a preparation method and application thereof, belonging to the field of anti-fatigue health care products. The composition for resisting fatigue and improving immunity comprises the following components: coenzyme Q10 and grape seed extract. According to the invention, coenzyme Q10 is combined with various natural antioxidant substances, the anti-fatigue activity of different compositions is investigated, and finally, the composition consisting of coenzyme Q10 and grape seed extract is found to have the most remarkable anti-fatigue activity. The invention further determines the optimal dosage ratio of the coenzyme Q10 and the grape seed extract through a dosage screening experiment. Animal experiments and biochemical index measurement results show that the composition has extremely obvious functions of resisting fatigue activity and enhancing immunity. The composition can be used for preparing medicines, health-care products, beverages or foods and the like for resisting fatigue and improving immunity, and has good safety and no side effect.
Description
Technical Field
The invention relates to a composition for resisting fatigue and improving immunity, in particular to a composition which takes coenzyme Q10 and grape seed extract as active ingredients and has the effects of resisting fatigue and improving immunity of a human body, and also relates to a preparation method and application of the composition, belonging to the field of health care products for resisting fatigue and improving immunity.
Background
Coenzyme Q10 (CoQ), also known as ubiquinone (UQ, Q), is a coenzyme loosely bound to proteins in the respiratory chain, is a fat-soluble quinone compound widely present in the body, and plays an important role in proton translocation and electron transfer in the respiratory chain of the human body. Coenzyme Q10 acts as an activator of cellular metabolism and cellular respiration and is also an important antioxidant and non-specific immunopotentiator. Coenzyme Q10 has two forms, oxidized form and reduced form. After the oxidized coenzyme Q10 enters the human body, the dehydrogenase needs to be converted once again, and the converted form is called reduced coenzyme Q10, which is more directly utilized by the human body, so that the reduced coenzyme Q10 has more direct action in the metabolism of the human body compared with the oxidized coenzyme Q10.
Fatigue is a complex physiological and biochemical change process of an organism and is a normal physiological phenomenon which is inevitably generated when human brain power or physical strength reaches a certain stage. The occurrence of fatigue can cause the reduction of the exercise capacity, the reduction of the working efficiency and the increase of error accidents; if the fatigue can not be recovered in time after the occurrence of the fatigue, the fatigue can be gradually accumulated, the overstrain syndrome, the chronic fatigue syndrome and the like can also be caused, so that the endocrine disturbance, the immunity decline and even the organic lesion of the organism can be caused, and the fatigue becomes an important factor for threatening the health of human beings.
Therefore, the development of the composition with definite anti-fatigue and immunity-improving effects has important market value and social significance.
Disclosure of Invention
The invention aims to solve the technical problem of providing the composition for resisting fatigue and improving immunity, which takes the coenzyme Q10 and the grape seed extract as active ingredients and has obvious effects of resisting fatigue and improving the immunity of a human body.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
the invention firstly discloses a composition for resisting fatigue and improving immunity, which comprises the following components: coenzyme Q10 and grape seed extract.
Coenzyme Q10 by mass ratio: grape seed extract ═ (1-15): (1-20); preferably, coenzyme Q10: grape seed extract ═ 1: 11-15; most preferably, coenzyme Q10: grape seed extract ═ 1: 13.
the coenzyme Q10 is reduced coenzyme Q10; compared with oxidized coenzyme Q10, reduced coenzyme Q10 has higher absorptivity and bioavailability, and can reach the same in vivo level by only needing smaller dose.
The reduced coenzyme Q10 can be purchased commercially, synthesized chemically, or extracted and separated from animals and plants, and the extraction method is well known to those skilled in the art. The grape seed extract may be purchased commercially or extracted from grape seeds by methods well known to those skilled in the art.
The invention combines reduced coenzyme Q10 with a plurality of natural antioxidant substances, namely tea polyphenol, vitamin C, vitamin E, soybean extract, grape seed extract and the like according to a certain proportion, carries out a mouse weight swimming experiment, and investigates the anti-fatigue activity of different compositions. The results show that the combination of the reduced coenzyme Q10 and different natural antioxidant substances has different degrees of anti-fatigue effect. The composition of the reduced coenzyme Q10 and the grape seed extract has very obvious anti-fatigue activity, the difference between the endurance time of a mouse and a blank control group is very obvious (P is less than or equal to 0.001), and the anti-fatigue activity is obviously superior to the combination of the reduced coenzyme Q10 and other natural antioxidant substances.
On the basis of screening out the reduced coenzyme Q10 and the grape seed extract as the components of the composition, the invention further optimizes the dosage and the proportion of the reduced coenzyme Q10 and the grape seed extract. Screening reduced coenzyme Q10 and a grape seed extract according to a mass ratio of 1: the composition consisting of 13 has extremely obvious anti-fatigue activity, the difference between the endurance time of the mice in the group and the blank control group is extremely obvious (P is less than or equal to 0.001), and the anti-fatigue activity is obviously superior to that of other dosage combinations.
The invention determines the biochemical indexes of fatigue resistance, and the results show that the reduced coenzyme Q10 and the grape seed extract are mixed according to the mass ratio of 1: 13 the serum lactic acid, urea nitrogen and MDA levels of the composition are significantly lower than those of the blank control group, while the levels of superoxide dismutase (SOD), glutathione reductase (GSH-PX) and liver glycogen are significantly higher than those of the blank control group. From the viewpoint of biochemical indexes, it is also proved that the reduced coenzyme Q10 and the grape seed extract are mixed according to the mass ratio of 1: 13 has extremely remarkable anti-fatigue activity.
The composition has the effects of resisting fatigue and enhancing immunity. The experimental result of detecting the influence of the composition consisting of the reduced coenzyme Q10 and the grape seed extract in different mass ratios on the delayed type allergy (DTH) and the number of antibody-producing cells of mice shows that the reduced coenzyme Q10 and the grape seed extract are mixed according to the mass ratio of 1: 13 can also obviously improve the delayed type allergic reaction capability and the number of antibody generating cells (P is less than or equal to 0.05) of the mice, which indicates that the composition also has very good function of enhancing the immunity.
On the basis of the composition for resisting fatigue and improving immunity, the composition can be added with proper auxiliary materials or carriers to prepare medicines, health-care products, drinks or foods for resisting fatigue and improving immunity; wherein the dosage form of the medicine or the health-care product comprises: tablet, granule, pill, emulsion, granule, soft capsule, hard capsule or microcapsule.
The carrier or the auxiliary material refers to a carrier or an auxiliary material which is conventional in the pharmaceutical field, such as: diluents, disintegrants, lubricants, excipients, binders, glidants, fillers, surfactants, and the like; in addition, other adjuvants such as flavoring agents and sweeteners may also be added to the composition.
The diluent may be one or more ingredients that increase the weight and volume of the tablet; common diluents include lactose, starch, pregelatinized starch, microcrystalline cellulose, sorbitol, mannitol, and inorganic calcium salts. The most common of them are lactose, starch, microcrystalline cellulose.
The disintegrant can be one or more of crosslinked polyvinylpyrrolidone (with a total weight ratio of 2-6%), crosslinked sodium carboxymethylcellulose (with a total weight ratio of 2-6%), alginic acid (with a total weight ratio of 2-5%), and microcrystalline cellulose (with a total weight ratio of 5-15%). Wherein the preferred ratio is crosslinked polyvinylpyrrolidone (2-7% by weight) and crosslinked sodium carboxymethylcellulose (2-6% by weight). Most preferably crosslinked polyvinylpyrrolidone (in a ratio of 2-6% by weight relative to the total weight).
The lubricant comprises one or a mixture of stearic acid, sodium stearate, magnesium stearate, calcium stearate, polyethylene glycol, talcum powder and hydrogenated vegetable oil. Magnesium stearate is most preferred. The amount of the lubricant is in the range of 0.10 to 1% (by total weight), and is generally 0.25 to 0.75%, and preferably 0.5 to 0.7%.
The binder may be one or more ingredients that facilitate granulation. It may be starch slurry (10-30% by weight of the total binder), hydroxypropyl methylcellulose (2-5% by weight of the total binder), polyvinylpyrrolidone (2-20% by weight of the total binder), preferably ethanol aqueous solution of polyvinylpyrrolidone, and most preferably 50% ethanol aqueous solution of polyvinylpyrrolidone.
The glidant can be one or a mixture of more of superfine silica gel powder, talcum powder and magnesium trisilicate.
The surfactant may be one or more components that improve wetting and increase drug dissolution. Sodium lauryl sulfate is often used (the usual range is 0.2-6% by weight, based on the total weight).
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
the invention combines the reduced coenzyme Q10 with a plurality of natural antioxidant substances, and screens out the anti-fatigue and immunity-improving composition consisting of the reduced coenzyme Q10 and the grape seed extract, the combination of the two has obvious synergistic effect, and the combination of the two according to a specific dosage proportion can obtain extremely obvious anti-fatigue and immunity-improving effects, and the invention has no adverse reaction and good safety.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It is to be understood that the described embodiments are exemplary only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be within the scope of the invention.
1. Raw materials
Reduced coenzyme Q10 was purchased from Western Anwei Olympic Biotech Ltd; the grape seed extract is purchased from Xianchang Yue plant chemical Co., Ltd; tea polyphenols are purchased from Wuxi Lubao biological products, Inc.; vitamin C and vitamin E were purchased from Guangzhou Qiyun Biotech, Inc.; propolis is purchased from Changsha Firpole plants GmbH; the sargassum fusiforme polysaccharide is prepared by a method of a reference document (Zhoujing. extraction, separation and activity research of natural antioxidant substances. 2008. Zhejiang university. Master academic thesis); the ginseng extract is purchased from Shaanxi brocade bioengineering GmbH; the soybean extract, the silybum marianum extract and the ginger extract are purchased from Shanxi Longfu biochemical engineering Co., Ltd.
EXAMPLE 1 preparation of anti-fatigue and Immunity-enhancing composition
Weighing the following raw materials (g): reduced coenzyme Q101 and grape seed extract 13 are evenly mixed to obtain the grape seed extract.
EXAMPLE 2 preparation of anti-fatigue and Immunity-enhancing composition
Weighing the following raw materials (g): reduced coenzyme Q101 and grape seed extract 11 are evenly mixed to obtain the grape seed extract.
EXAMPLE 3 preparation of anti-fatigue and Immunity-enhancing composition
Weighing the following raw materials (g): reduced coenzyme Q101 and grape seed extract 15 are evenly mixed to obtain the grape seed extract.
EXAMPLE 4 preparation of anti-fatigue and Immunity-enhancing composition
Weighing the following raw materials (g): reduced coenzyme Q101 and grape seed extract 20 are evenly mixed to obtain the grape seed extract.
EXAMPLE 5 preparation of anti-fatigue and Immunity-enhancing composition
Weighing the following raw materials (g): reducing coenzyme Q1015 and grape seed extract 1, and mixing uniformly to obtain the final product.
EXAMPLE 6 preparation of Soft capsules
Uniformly mixing 200g of glycerol, 320g of gelatin, 450g of purified water and 2g of pigment, heating for dissolving, defoaming in vacuum, standing and preserving heat to obtain a gelatin solution; 60g of the composition prepared in the example 1 is heated to be dissolved, then mixed with the gelatin melting liquid to be made into pills according to the soft capsule bundling process, and the soft capsules are obtained after shaping, washing, drying and picking up the pills.
EXAMPLE 7 preparation of tablets
15g of the composition prepared in the embodiment 2 is added with 15g of starch, evenly mixed, then evenly mixed with 30 g of dextrin, and added with a proper amount of ethanol (50%) to prepare a soft material; granulating through a 16-mesh nylon sieve, drying at 60 ℃, sieving the dried granules again through a 16-mesh sieve for finishing granules, adding 5g of magnesium stearate, uniformly mixing and tabletting.
EXAMPLE 8 preparation of granules
Taking 3 g of the composition prepared in example 3, mixing with 5g of microcrystalline cellulose, 3 g of lactose and 1g of croscarmellose sodium, preparing a soft material by taking 3% povidone-ethanol solution (with the concentration of 50%) as a binding agent, sieving with a 40-mesh sieve, granulating, drying at 60 ℃, sieving with a 30-mesh sieve, and finishing granules to obtain the granules.
Experimental example 1 composition screening experiment for anti-fatigue and immunity improvement
1. Experimental methods
1.1 formulation of the composition
Respectively combining reduced coenzyme Q10 with natural antioxidant substances such as tea polyphenols, vitamin C, vitamin E, propolis, herba Silybi Mariani extract, Cyrtymenia Sparsa polysaccharide, semen glycines extract, grape seed extract, Ginseng radix extract, and rhizoma Zingiberis recens extract, and mixing well to obtain the composition. Wherein the mass ratio of the reduced coenzyme Q10 to the natural antioxidant substances is 1: 10.
1.2 animal groups and types and dosages of administration
Male ICR mice (20 ± 1g) at 7 weeks of age (beijing weitonglihua laboratory animal technology ltd), laboratory animal certification No.: SCXK (Jing) 2012 and 0001. Mice were randomly divided into blank control groups and experiment 1-10 groups of 12 mice each. The test drug is administrated to the mice by intragastric administration once a day, the dose is 0.15g/Kg, and the mice are intragastric administered for 30 days.
Blank control group: perfusing stomach with normal saline;
experiment 1 group: administering a composition consisting of reduced coenzyme Q10 and tea polyphenol for intragastric administration;
experiment 2 group: administering a composition of coenzyme Q10 and vitamin C, and performing intragastric administration;
experiment 3 groups: administering a composition of coenzyme Q10 and vitamin E for intragastric administration;
experiment 4 groups: administering coenzyme Q10 and propolis composition, and intragastrically;
experiment 5 group: administering a composition consisting of coenzyme Q10 and Silybum marianum extract, and intragastrically;
experiment 6 groups: administering a composition consisting of coenzyme Q10 and sargassum fusiforme polysaccharide, and performing intragastric administration;
experiment 7 groups: administering a composition comprising coenzyme Q10 and soybean extract, and intragastrically;
experiment 8 groups: administering a composition comprising coenzyme Q10 and grape seed extract, and intragastrically;
experiment 9 groups: administering coenzyme Q10 and Ginseng radix extract composition, and intragastrically;
experiment 10 groups: administering coenzyme Q10 and rhizoma Zingiberis recens extract composition, and intragastric irrigating.
1.3 mouse weight bearing swimming experiment
The detection method comprises the following steps: after 30 minutes of the last administration, randomly extracting 1 mouse from each group with a lead skin with the weight of 3 percent corresponding to the tail part of the mouse, putting the mice into water to start timing, and recording the first sinking time and exhaustion time of the mice till the exhaustion of the mice is finished, wherein the difference of the two times represents the endurance capacity of the mice;
detecting a container: adopting a bucket with the diameter of 43cm and the height of 40cm, wherein the water level is 25cm, the water temperature is 25 +/-1 ℃, and 4 buckets are used; detection standard: the first sinking standard is that the nose tip of the mouse firstly sinks below the water surface; the exhaustion standard is that the mice do not float upwards after sinking underwater for 10 s.
1.4 statistical methods
The data processing is carried out by adopting SPSS statistical analysis software, and all the data are calculated as the mean value plus or minus standard error
Indicated, differences between groups were treated with One-way ANOVA.
2. Results of the experiment
The experimental results are shown in Table 1, and it can be seen that the reduced coenzyme Q10 has different degrees of anti-fatigue effects when combined with different natural antioxidant substances. The composition of the experiment 8 group, namely the reduced coenzyme Q10 and the grape seed extract, has very obvious anti-fatigue activity, the endurance time of the mice in the group is very different from that of a blank control group (P is less than or equal to 0.001), and the anti-fatigue activity is obviously superior to that of the reduced coenzyme Q10 and other natural antioxidant substances.
TABLE 1 anti-fatigue test results for different compositions
P ≦ 0.05, statistically different from the blank group;
p ≦ 0.01, statistically significantly different from the blank group;
indicates P ≦ 0.001, with a very significant statistical difference compared to the blank group.
Experimental example 2 screening experiment for formula dosage of composition for anti-fatigue and enhancing immunity
1. Experimental methods
1.1 formulation of the composition
The invention combines reduced coenzyme Q10 and grape seed extract according to different dosage proportions on the basis of experimental example 1 (table 2), and carries out mouse swimming-bearing experiments according to the method of experimental example 1.
TABLE 2 different ratios of the compositions
2. Results of the experiment
The experimental results are shown in Table 3, and it can be seen that different combinations of reduced coenzyme Q10 and grape seed extract have different degrees of anti-fatigue effects. Wherein the mass ratio of reduced coenzyme Q10 to grape seed extract is 1: the composition consisting of 13 has obvious anti-fatigue activity, the difference between the endurance time of the mice in the group and the blank control group is very obvious (P is less than or equal to 0.001), and the anti-fatigue activity is obviously superior to that of the combination with other dosage.
TABLE 3 anti-fatigue test results for different compositions
P ≦ 0.01, statistically significantly different from the blank group;
indicates P ≦ 0.001, with a very significant statistical difference compared to the blank group.
Experimental example 3 measurement experiment of biochemical indexes of fatigue resistance
1. Experimental methods
After the mice in experiment example 2 were subjected to exhaustive swimming test for 10 minutes, the mice in the blank control group and experiment 6 group were immediately picked up to take blood from the eyeballs, and the liver tissues were dissected and taken out. Standing the blood in a refrigerator at 4 deg.C overnight, centrifuging at 3000r/min for 10min the next day, and collecting serum; the liver tissue is stored at-80 deg.C for use.
And (3) determination of biochemical indexes: serum was used for the determination of blood lactate and urea nitrogen, and liver was used for the determination of liver glycogen, Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione reductase (GSH-PX). All biochemical kits are provided by Nanjing institute of bioengineering, and the determination method is carried out according to the kit instruction.
2. Results of the experiment
As can be seen from table 4, reduced coenzyme Q10 and grape seed extract were mixed in a mass ratio of 1: 13, the serum lactic acid, urea nitrogen and MDA levels were significantly lower than those of the placebo group, while the superoxide dismutase (SOD), glutathione reductase (GSH-PX) and liver glycogen levels were significantly higher than those of the placebo group. From the viewpoint of biochemical indexes, it is also proved that the reduced coenzyme Q10 and the grape seed extract are mixed according to the mass ratio of 1: 13 has extremely remarkable anti-fatigue activity.
TABLE 4 Biochemical index of anti-fatigue test results
P ≦ 0.05, statistically different from the blank group;
p ≦ 0.01, statistically significant compared to the blank group.
Experimental example 4 detection of immunity-enhancing function of composition for anti-fatigue and enhancing immunity
1. Experimental methods
Reduced coenzyme Q10 and grape seed extract were used to prepare compositions according to Table 2 of Experimental example 2, and mice were randomly divided into a blank control group and experiment groups 1-14, each group containing 10 mice, and they were gavaged once daily for 30 days according to the method and dose of Experimental example 1.
1.1 delayed type allergic reaction (DTH) (foot-cast thickening method)
After the mice were continuously given for 30 days, the plantar thickness of the left hind foot was measured 4 days after the mice were intraperitoneally injected with 2% (v/v) SRBC (0.2m 1/per mouse) for sensitization, and then the plantar thickness of the left hind foot was measured 24 hours after the injection by subcutaneously injecting 20% (v/v) SRBC (20. mu.l/per mouse) at the measurement site, and the thickness of the plantar portion of the left hind foot was measured three times at the same site and averaged. The degree of DTH is expressed as the difference in thickness of the plantar region of the foot (degree of swelling of the plantar region) before and after the attack.
1.2 measurement of antibody-producing cells (Jerne modified slide method)
After the mice are continuously given samples for 30 days, sheep blood is taken and washed 3 times by physiological saline, each time, the sheep blood is centrifuged (2000r/min) for l0min, the overstocked SRBC is prepared into 2 percent (v/v) cell suspension by the physiological saline, and each mouse is injected with 0.2m1 in the abdominal cavity. After 4 days, the mice were sacrificed, the spleen was taken, gently ground, made into a cell suspension using Hank's solution, sieved with a 200-mesh sieve, washed, centrifuged 2 times, and finally suspended in 8m1Hank's solution, the cells were counted, and the cell concentration was adjusted to 5X 106One per ml. Heating to dissolve surface layer culture medium, mixing with equal amount of 2 times concentration of Hank's solution with pH of 7.4, subpackaging in small tubes of 0.5m1, adding 10% SRBC 50 μ l (v/v) prepared by SA solution and 20 μ 1 spleen cell suspension (5 × 10)6Pieces/ml), quickly mixing, pouring onto slide glass brushed with thin layer of agarose, horizontally buckling the slide glass on a slide glass rack after agarose is solidified, placing the slide glass in a carbon dioxide incubator for incubation for 1.5h, and then using the slide glassComplement diluted in SA solution (1: 8) was added to the slide well and the number of lyso-plaques counted after 1.5h of incubation was continued.
2. Results of the experiment
2.1 Effect of the composition on delayed hypersensitivity (DTH) in mice
The influence of the composition on the delayed allergy (DTH) of the mouse is shown in Table 5, and the difference between the influence of the experiment 6 groups on the swelling degree of the foot sole of the mouse and the control group is obvious (P is less than or equal to 0.05), which indicates that the composition for resisting fatigue and improving immunity has the capability of improving the delayed allergy (DTH) of the mouse.
TABLE 5 Effect of compositions on delayed allergy (DTH) in mice
Denotes P ≦ 0.05 with statistical differences compared to the blank group.
2.2 antibody-producing cell assay results
The influence of the composition on the number of mouse antibody-producing cells is shown in Table 6, and the ratio of the influence of the experiment 6 groups on the number of plaques to that of a control group is remarkably different (P is less than or equal to 0.05), so that the composition for resisting fatigue and improving immunity has the capability of increasing the number of mouse antibody-producing cells.
TABLE 6 Effect of compositions on mouse antibody-producing cell number
Denotes P ≦ 0.05 with statistical differences compared to the blank group.
In conclusion, the anti-fatigue and immunity-improving composition can obviously improve the delayed type allergic reaction capability of mice and the number of antibody-producing cells (P is less than or equal to 0.05), and the composition has the function of improving the immunity.
Claims (6)
1. The composition for resisting fatigue and improving immunity is characterized by being prepared from the following components: coenzyme Q10 and grape seed extract;
coenzyme Q10 by mass ratio: grape seed extract ═ 1: 13;
the coenzyme Q10 is reduced coenzyme Q10.
2. A method of preparing the composition of claim 1, comprising: weighing the components according to the proportion, and uniformly mixing to obtain the composition.
3. Use of the composition of claim 1 for the preparation of an anti-fatigue medicament, nutraceutical, beverage or food product.
4. Use of the composition of claim 1 for the preparation of a medicament, health product, beverage or food for enhancing immunity.
5. Use according to claim 3 or 4, wherein the medicament is in a dosage form comprising: tablets, granules, pills, emulsions, granules or capsules; the capsule is soft capsule, hard capsule or microcapsule.
6. Use according to claim 3 or 4, wherein the formulation of the nutraceutical comprises: tablets, granules, pills, emulsions, granules or capsules; the capsule is soft capsule, hard capsule or microcapsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510286099.0A CN104857180B (en) | 2015-05-29 | 2015-05-29 | Composition for resisting fatigue and improving immunity and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510286099.0A CN104857180B (en) | 2015-05-29 | 2015-05-29 | Composition for resisting fatigue and improving immunity and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104857180A CN104857180A (en) | 2015-08-26 |
| CN104857180B true CN104857180B (en) | 2020-02-14 |
Family
ID=53903645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510286099.0A Active CN104857180B (en) | 2015-05-29 | 2015-05-29 | Composition for resisting fatigue and improving immunity and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104857180B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106177227A (en) * | 2016-08-25 | 2016-12-07 | 成都润馨堂药业有限公司 | A kind of compositions containing coenzyme Q10 strengthening body immunity |
| CN110150633A (en) * | 2017-12-25 | 2019-08-23 | 江南大学（如皋）食品生物技术研究所 | Compound anti-oxidation functional food additive and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322711A (en) * | 2008-01-09 | 2008-12-17 | 济南老来寿生物技术有限公司 | Health care medicament for resisting fatigue and enhancing immunity and preparation thereof |
| CN103300373A (en) * | 2013-06-26 | 2013-09-18 | 重庆艾视生物科技有限公司 | Lutein and procyanidine composite soft capsule and preparation method thereof |
-
2015
- 2015-05-29 CN CN201510286099.0A patent/CN104857180B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322711A (en) * | 2008-01-09 | 2008-12-17 | 济南老来寿生物技术有限公司 | Health care medicament for resisting fatigue and enhancing immunity and preparation thereof |
| CN103300373A (en) * | 2013-06-26 | 2013-09-18 | 重庆艾视生物科技有限公司 | Lutein and procyanidine composite soft capsule and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104857180A (en) | 2015-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101940620B (en) | Medicinal composition for treating diabetes mellitus and application thereof | |
| CN108420854B (en) | Antifatigue composition for improving endurance performance | |
| CN103145679B (en) | Preparation method and application of epigallocatechin gallate | |
| CN111035649B (en) | NMN + GLP compound nutritional supplement and preparation method and application thereof | |
| CN108042627B (en) | Composition for treating hyperuricemia and preparation method and application thereof | |
| CN104224885A (en) | Traditional Chinese medicine composition for relieving physical fatigue | |
| CN101380346B (en) | Traditional Chinese composition for treating tumor and production method thereof | |
| CN105341893A (en) | Composition for assisting in reducing blood glucose and application | |
| CN102526346B (en) | Health-care pharmaceutical formulation with immunity-enhancing and senility-delaying functions | |
| CN104857180B (en) | Composition for resisting fatigue and improving immunity and preparation method and application thereof | |
| CN102319256B (en) | Application of porphyra polysaccharide in preparing treatment medicament or prevention health-care product of kidney diseases | |
| CN101336709A (en) | Antifatigue nutrient preparation | |
| CN102657730A (en) | Rhodiola rosea buccal preparations for resisting altitude reaction | |
| CN102579530A (en) | Preparation method of aralia taibaiensis total saponin having diabetes mellitus resisting effect and medicament | |
| CN105106300A (en) | Use of cyclocarya paliurus extract in preparation of drug for preventing and treating non-alcoholic fatty liver disease | |
| US9943560B2 (en) | Medical compositions containing liquorice extracts with synergistic effect | |
| CN1686424A (en) | Medicinal composition containing scutellaria and bupleurum and its preparation method | |
| TWI612968B (en) | A use of an extract of asplenium australasicum (j. sm.) hook. | |
| KR20150031373A (en) | Phamaceutical and food composition for preventing or treating obesity comprising extract of leaf from Hoppophea rhamnoids as effective component | |
| CN109394799B (en) | Natural pharmaceutical composition containing ganoderma lucidum extract and preparation method and application thereof | |
| CN110882319A (en) | Application of thoroughfare bitter orange, thoroughfare bitter orange extract and products containing thoroughfare bitter orange extract in preventing and/or treating metabolic liver diseases | |
| KR101093006B1 (en) | Method for producing functional health food containing bezoar bovis used microbes and the functional health food produced by the same | |
| CN101032534A (en) | Method of preparing jiubiying total saponins and the application thereof | |
| CN104258034A (en) | Traditional Chinese medicine composition for boosting immunity | |
| CN104352672A (en) | Tortoise plastron-containing traditional Chinese medicine composition for treating diabetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |