CN112649241A - Adsorption type root system secretion collecting system - Google Patents
Adsorption type root system secretion collecting system Download PDFInfo
- Publication number
- CN112649241A CN112649241A CN202011485298.1A CN202011485298A CN112649241A CN 112649241 A CN112649241 A CN 112649241A CN 202011485298 A CN202011485298 A CN 202011485298A CN 112649241 A CN112649241 A CN 112649241A
- Authority
- CN
- China
- Prior art keywords
- secretion
- root
- collecting
- column
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
An adsorption type root secretion collecting system is characterized in that a plant cultivation groove is connected with the side wall of a secretion collecting groove, so that plant roots planted in the plant cultivation groove enter the secretion collecting groove, after culture liquid is filled into the secretion collecting groove for cultivation, a peristaltic pump is started, the culture liquid in the secretion collecting groove is extracted, the culture liquid flows back to the secretion collecting groove after passing through a secretion adsorption column to complete circulation collection, and a plurality of rows of secretion adsorption collecting units are arranged in a middle cavity of the secretion collecting groove in parallel; the invention utilizes the secretion absorption and collection unit in the secretion collection tank to absorb the root secretions, and utilizes the culture solution to dissolve and carry the root secretions absorbed in the secretion absorption and collection unit out of the secretion collection tank and then absorb the root secretions by the resin column in the absorption column, thereby not only simulating the natural environment for root growth, but also not needing to dig out damaged root systems from plant root systems, the collection method is relatively simple and convenient, and a large amount of collection can be repeated in a period of time.
Description
Technical Field
The invention relates to the research field of plant root exudates, in particular to an adsorption type root exudates collecting system.
Background
The root secretion refers to a wide variety of substances secreted or released from different parts of the root system into the growth medium in the growth process of plants, and the substances include organic substances with low relative molecular mass, macromolecular mucilage substances, root cell desquamation products, decomposition products of the root cell desquamation products, gas, protons, nutrient ions and the like. The root exudates are not only considerable in quantity, but also very effective, and are a key factor for maintaining the vitality of the rhizosphere micro-ecosystem, and are also an important component of rhizosphere material circulation. The root exudates remarkably change physical, chemical and biological properties of a root-soil interface, have important effects on aspects such as soil structure formation, soil nutrient activation, plant nutrient absorption, environmental stress relief and the like, and the research on the root exudates is important content in research fields such as plant nutrition, allelopathy, biological pollution stress, environmental pollution remediation and the like and is generally concerned by scholars at home and abroad.
In the prior art, the collection of root exudates mainly comprises a water culture method, a matrix culture collection method and a soil culture method. The water culture method does not need a special collecting device, but the water culture plants generally have less root hair development and no mechanical resistance, and have larger differences from the aeration condition, the nutrient distribution and the root branch structure of the growth under the real soil culture condition. Therefore, the water culture method cannot obtain the root exudates of the xerophyte. The substrate culture method can ensure the ventilation of the root system and has certain mechanical resistance, but has larger difference with the root system secretion in the natural state.
The traditional soil culture method is mainly used for collecting root exudates by two methods, one is that after the soil culture plant grows for a period of time, the root system of the plant is taken out, the rhizosphere soil is washed by distilled water, and the obtained product is centrifuged or filtered after being vibrated, so that the obtained root exudates are obtained. And secondly, taking the plant growing to a certain stage out of the soil, washing the soil layer on the surface of the root system, and then rinsing the root system with distilled water, wherein the rinsing liquid is used as root secretion. During the whole operation process, the root system is extremely easy to damage, the collected solution contains a plurality of inclusions and bleeding liquid of the root system, and the method is generally only suitable for small plants or seedling-stage plants.
Disclosure of Invention
In order to solve the problems existing in the traditional soil culture method for collecting plant root exudates, the invention provides an adsorption type root exudates collecting system, wherein the root systems in the plant growth process are induced to enter a special-structure exudates collecting tank in a centralized manner, the exudates adsorbing and collecting unit in the exudates collecting tank is used for adsorbing the root exudates, and the root exudates adsorbed in the exudates adsorbing and collecting unit are dissolved and taken out of the exudates collecting tank by using culture solution and then are adsorbed by a resin column in an adsorption column.
The technical scheme adopted by the invention for solving the technical problems is as follows: an adsorption type root system secretion collecting system comprises a plant cultivation tank, a secretion collecting tank, a secretion adsorption column and a peristaltic pump, wherein the plant cultivation groove is connected with the side wall of the secretion collecting groove, so that the plant root system planted in the plant cultivation groove enters the secretion collecting groove, after culture solution is filled into the secretion collecting tank for culture, a peristaltic pump is started to pump the culture solution in the secretion collecting tank, so that the culture solution flows back to the secretion collecting tank after passing through a secretion adsorption column to complete circular collection, wherein the secretion collecting tank is a closed tank body, the upper partition plate and the lower partition plate which are horizontally arranged in the device divide the inner cavity into an upper cavity chamber, a middle cavity chamber and a lower cavity chamber which are separated from each other, the middle cavity is communicated with the plant cultivation groove through a through groove formed in the side wall of the middle cavity, so that a plant root system in the plant cultivation groove enters the middle cavity through the through groove;
the middle chamber is provided with a plurality of rows of secretion adsorption and collection units in parallel, each row of root secretion adsorption and collection units comprises a plurality of secretion collection columns distributed along the height direction of the middle chamber, a gap is formed between every two adjacent secretion collection columns, and the upper end and the lower end of each secretion collection column penetrate through the upper partition plate and the lower partition plate respectively and then extend into the upper chamber and the lower chamber to form a culture solution channel communicated with the upper chamber and the lower chamber; sterile river sand or sterile glass beads are filled in gaps between adjacent secretion adsorption and collection units and between adjacent secretion collection columns to form root system culture soil;
plant root system in the lumen can prick into secretion collection post internal growth or hug closely secretion collection post growth in the growth process to make root system secretion attached to secretion collection post surface or get into inside the secretion collection post, the culture solution of injecting into in the lumen can follow secretion collection post downstream and enter into in the lumen, and then carry plant roots secretion along secretion collection post reentrant cavity in, finally adsorb by the resin column in it behind the secretion adsorption column under the drive of peristaltic pump.
As an optimal scheme of the adsorption type root system secretion collecting system, the plant cultivation grooves are two groove bodies symmetrically arranged on two sides of the secretion collecting groove, each groove body comprises a vertical part for planting plants, and the bottom of the vertical part is communicated with the secretion collecting groove through a root system guiding part.
As another preferable mode of the adsorption type root exudate collecting system, a bottom wall of the root system guiding part is obliquely arranged and gradually rises from the bottom of the vertical part to one side of the exudate collecting tank; the lowest part of the vertical part is provided with a liquid drainage air hole.
As another preferred scheme of the above adsorption type root exudate collecting system, a culture liquid tank is arranged above the root guide part, the culture liquid tank is a U-shaped structure surrounded by a side wall of the vertical part, a top wall of the root guide part and a side wall of the upper chamber, an opening of the U-shaped structure is sealed by a sealing plate to form a sealed culture liquid tank, a plurality of liquid permeable holes are distributed on the top wall of the root guide part, the liquid permeable holes drip culture liquid in the culture liquid tank into the root guide part, and tend to be preferentially diffused near the vertical part under the action of gravity factors to guide the root system of the plant planted in the vertical part to grow towards the root guide part; the surface of the sealing plate is provided with a culture solution injection port for filling culture solution into the culture solution groove.
As another preferable scheme of the adsorption type root system secretion collecting system, the secretion collecting column is a strip-shaped object formed by wet mixing a plurality of strands of bast fiber bundles arranged along the length direction of the secretion collecting column and matrix soil, a layer of plastic film is wrapped outside the strip-shaped object, two ends of the strip-shaped object are wrapped with non-woven fabric layers to prevent the matrix soil from losing, and the matrix soil is formed by mixing vermiculite powder with the particle size not more than 1mm, river sand particles with the particle size not more than 2mm and peat soil in a mass ratio of 6:1: 3; the diameter of the bast fiber bundle is 1mm, the thickness of the plastic film is 0.1mm, the diameter of the secretion collecting column is 1cm, and the length is 20-30 cm.
As another preferable mode of the adsorption type root system secretion collecting system, a culture solution input pipe and a culture solution circulation injection pipe are arranged at the top of the upper chamber, wherein the culture solution input pipe is used for injecting culture solution for circulation into the upper chamber, and the culture solution circulation injection pipe is connected with a peristaltic pump through a hose.
As another preferable mode of the above adsorption type root exudate collecting system, a gauze is provided on the through groove of the middle chamber, and the gauze is used for preventing the cultivated soil in the root system guiding part from entering the middle chamber, but allowing the root system to pass through the gauze to enter the middle chamber.
In another preferred embodiment of the adsorption-type root exudate collection system, a bottom wall of the lower chamber is inclined from one side to the other side, and a drain pipe is provided at the lowest position; and a filter cloth is arranged below the bottom end of the endocrine collection column in the lower chamber and used for filtering culture solution seeped by the endocrine collection column.
As another preferable scheme of the adsorption type root system secretion collecting system, a plurality of rows of mounting grooves are correspondingly formed in the upper separation plate and the lower separation plate, each row of secretion adsorption collecting units further comprises an upper mounting plate and a lower mounting plate, the upper ends and the lower ends of all secretion collecting columns in the secretion adsorption collecting units respectively penetrate through the upper mounting plate and the lower mounting plate, the upper mounting plate on each row of secretion adsorption collecting units is fixed in the mounting groove of the upper separation plate, and the lower mounting plate is fixed in the mounting groove of the lower separation plate; soil filling grooves are distributed at two ends of the mounting groove on the upper partition plate, and root culture soil is filled in the middle chamber through the soil filling grooves.
As another preferred scheme of above-mentioned absorption formula root system secretion collection system, the secretion adsorption column includes the vertical body of a both ends open-ended, and the middle part symmetry at vertical body inner wall is provided with two snap rings, and these two snap rings cooperate with the cock body that vertical body both ends set up to fix nonpolar macroporous resin column and polarity macroporous resin column in vertical body respectively, and form the liquid buffer region between nonpolar macroporous resin column and polarity macroporous resin column, cock body and vertical body threaded connection, and the middle part of cock body all is provided with the liquid pipe, and two liquid pipes of vertical body top and bottom communicate with lower cavity through hose and peristaltic pump respectively, and are provided with the filter pulp between the cock body and the polarity macroporous resin column of vertical body bottom.
Compared with the prior art, the invention has the following beneficial effects:
1) the root systems in the plant growth process are induced to intensively enter the secretion collecting tank with a special structure, the secretion absorbing and collecting unit in the secretion collecting tank is used for absorbing root secretion, and the culture solution is used for dissolving the root secretion absorbed in the secretion absorbing and collecting unit and carrying the dissolved root secretion out of the secretion collecting tank and then absorbing the root secretion by the resin column in the absorption column, so that the natural environment for root system growth can be simulated, the root systems of the plants can be collected without digging out, the damage to the root systems is avoided, the collecting method is relatively simple and convenient, and a large amount of collection can be repeated in a period of time;
2) the secretion absorption and collection unit in the cavity divides the whole cavity into a plurality of gaps by utilizing a plurality of secretion collection columns, after root system culture soil is filled in the gaps, most of space of the whole cavity is occupied by the secretion collection columns, the root system can inevitably prick into the secretion collection columns to grow in the growth process of utilizing the culture soil, and the generated secretion can be dissolved in culture solution and then enters a lower cavity along with the secretion collection columns and is finally absorbed by the resin columns to finish the collection process, and the collection process is simple and convenient;
3) the plant cultivation tank is divided into a vertical part and a root system guide part, the root system guide part is inclined upwards, the culture solution is poured into the root system guide part by using the culture solution tank to promote the growth and development of the root system and guide the root system to enter the middle cavity for growth, the inclined upwards structure can enable the root system to grow against the gravity factor, the gathering effect of the root system is achieved, the root system is enabled to grow more intensively, the collection efficiency is improved, the redundant culture solution cannot enter the middle cavity, and the growth condition of collecting root system secretion is changed;
4) the main body of the secretion collecting column is a strip-shaped object formed by mixing the bast fiber bundles and matrix soil in a wet mode, the matrix soil is formed by vermiculite powder, river sand and peat soil and has good water permeability and adsorbability, the bast fiber bundles provide a culture solution water diversion channel, the substrate soil and the vermiculite powder, the river sand and the peat soil are matched with an externally wrapped plastic film to form a culture solution conveying channel, the plastic film can be punctured to enter the culture solution conveying channel to grow in the growth process of plant roots, and secretions can be naturally carried away by the culture solution and adsorbed by the resin column; in order to prevent the matrix soil from being carried away by the culture solution, the two ends of the strip-shaped objects are wrapped by the non-woven fabrics, and fiber holes of the non-woven fabrics can play a good role in blocking and filtering, so that the loss of the matrix soil is prevented, but the circulation of the culture solution is not prevented.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention in use;
FIG. 2 is a schematic structural view of the present invention;
FIG. 3 is a top view of FIG. 2;
FIG. 4 is a schematic diagram of a secretion collection tank;
FIG. 5 is a schematic view of the top separator plate;
FIG. 6 is a schematic view of the bottom divider plate configuration;
FIG. 7 is a schematic structural view of a secretion adsorbing and collecting unit;
FIG. 8 is a schematic diagram of the structure of a secretion collection column;
FIG. 9 is a schematic structural view of a secretion adsorbing column;
reference numerals: 1. secretion collecting tank 101, upper partition plate 102, lower partition plate 103, upper chamber 104, middle chamber 105, lower chamber 106, filter cloth 107, drain pipe 108, culture fluid input pipe 109, culture fluid circulation injection pipe 1010, penetration tank 1011, gauze 1012, mounting tank 1013, soil filling tank 2, secretion adsorption collecting unit 201, upper mounting plate 202, lower mounting plate 203, secretion collecting column 2031, plastic film 2032, phloem fiber bundle 2033, matrix soil 204, gap 3, plant cultivation tank 301, vertical part 302, root guide part 303, liquid drainage vent hole 4, culture fluid tank 401, closing plate 402, liquid permeation hole 403, culture fluid injection port 5, secretion adsorption column 501, vertical tube body 502, snap ring 503, liquid buffer zone 504, nonpolar macroporous resin column 505, polar macroporous resin column, 506. filter cotton 507, a plug body 508, a liquid pipe 6 and a peristaltic pump.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following specific embodiments. The plants planted in the plant cultivation tank can be planted and cultivated according to the research type, and the soil in the plant cultivation tank and the used culture solution are determined according to the planted plants.
Example 1
As shown in fig. 1-4 and 7, an adsorption type root system secretion collecting system comprises a plant cultivation tank 3, a secretion collecting tank 1, a secretion adsorption column 5 and a peristaltic pump 6, wherein the plant cultivation tank 3 is connected with a side wall of the secretion collecting tank 1, so that a plant root system planted in the plant cultivation tank 3 enters the secretion collecting tank 1, after culture liquid is poured into the secretion collecting tank 1 for cultivation, the peristaltic pump 6 is started to extract culture liquid in the secretion collecting tank 1, the culture liquid flows back to the secretion collecting tank 1 after passing through the secretion adsorption column 5 to complete circulation collection, the secretion collecting tank 1 is a closed tank body, an upper partition plate 101 and a lower partition plate 102 horizontally arranged in the secretion collecting tank 1 divide an inner cavity into an upper cavity 103, a middle cavity 104 and a lower cavity 105 which are separated from each other, wherein the middle cavity 104 is communicated with the plant cultivation tank 3 through a through groove 1010 formed on a side wall of the middle cavity 104, so that the plant roots in the plant cultivation tank 3 enter the middle chamber 104 through the through groove 1010;
the middle chamber 104 is provided with a plurality of rows of secretion adsorption and collection units 2 in parallel, each row of root secretion adsorption and collection units 2 comprises a plurality of secretion collection columns 203 distributed along the height direction of the middle chamber 103, a gap 204 is formed between every two adjacent secretion collection columns 203, the upper end and the lower end of each secretion collection column 203 penetrate through the upper partition plate 101 and the lower partition plate 102 respectively and then extend into the upper chamber 103 and the lower chamber 105, and a culture solution channel for communicating the upper chamber 103 with the lower chamber 105 is formed; sterile river sand or sterile glass beads are filled in gaps between adjacent secretion adsorption and collection units 2 and between adjacent secretion collection columns 203 to form root system culture soil;
plant root system in the middle cavity 104 can prick into secretion collection post 203 in-growth or hug closely secretion collection post 203 and grow in the growth process to make root system secretion attached to secretion collection post 203 surface or get into secretion collection post 203 inside, the culture solution of injecting into in the upper cavity 103 can follow secretion collection post 203 and move down and enter into middle cavity 104, and then carry plant root system secretion and follow secretion collection post 203 and reentrant the lower cavity 105 in, finally adsorb by its resin post in behind secretion adsorption column 5 under the drive of peristaltic pump 6.
When the invention is implemented, the plant cultivation tank 3 and the secretion collecting tank 1 can be both arranged into long strips, and then one or more rows of plants are planted in the plant cultivation tank 3 in an arrangement manner, so that the root systems of the plants can be converged into the secretion collecting tank 1, and the secretion collecting amount is improved; the secretion adsorption and collection units 2 in the middle chamber 104 are distributed along the length direction, the width of the gap 204 between adjacent secretion collection columns 203 is generally 1cm, and the distance between two adjacent rows of secretion adsorption and collection units 2 is also 1 cm; finally, root culture soil is filled between the secretion collecting columns 203, and the roots of the plants inevitably take root in the secretion collecting columns 203 during the growth process;
in the invention, the plant cultivation tank 3 and the secretion collecting tank 1 are both made of transparent organic glass, so that the growth distribution condition of the planted plant root system can be conveniently observed, when the plant root system is observed to enter the secretion collecting tank 1 in a large amount to grow (the root system is fully distributed in at least half of the volume of the middle cavity 104), the root system secretion starts to be collected, and the operation steps are as follows:
1) injecting a culture solution into the upper chamber 103 through the culture solution input pipe 108, allowing the culture solution in the upper chamber 103 to permeate into the lower chamber 105 and the middle chamber 104 through the secretion collecting column 203, observing the lower chamber 105, stopping injecting the culture solution into the upper chamber 103 until the lower chamber 105 is filled with the culture solution, and standing and culturing for 5-8 hours;
2) the peristaltic pump 6 is started to pump the culture solution in the lower chamber 105, the culture solution passes through the secretion adsorption column 5 and then is injected into the upper chamber 103 through the culture solution circulation injection pipe 109 to complete a circulation, and during the circulation, the root secretion is adsorbed by the resin column in the secretion adsorption column 5;
3) circulating for many times to finish collecting root exudates, taking down the resin column in the exudate adsorption column 5 and carrying out conventional elution operation to obtain the root exudates;
it should be noted that: the components of the culture solution, the operating parameters of the peristaltic pump 6, the selection of the resin column, the subsequent elution of the resin column, and the like can be selected and used by those skilled in the art according to actual situations, and are not described herein in detail.
The foregoing is a basic embodiment of the present invention, and further modifications, optimizations and limitations can be made on the foregoing, so as to obtain the following examples:
example 2
This embodiment is an improved scheme based on embodiment 1, and the main structure thereof is the same as embodiment 1, and the improvement point is that: as shown in fig. 1 to 3, the plant cultivation tank 3 is two tanks symmetrically arranged at both sides of the secretion collecting tank 1, each tank includes a vertical portion 301 for planting a plant, and the bottom of the vertical portion 301 is communicated with the secretion collecting tank 1 through a root system guide portion 302.
Example 3
The present embodiment is an improved scheme based on embodiment 2, and the main structure of the present embodiment is the same as that of embodiment 2, and the improvement point is that: as shown in fig. 2, the bottom wall of the root system guide portion 302 is disposed obliquely and gradually rises from the bottom of the vertical portion 301 to the side of the secretion collecting tank 1; a liquid discharge vent 303 is provided at the lowest position of the vertical portion 301.
In this embodiment, the drainage vent holes 303 may be formed at intervals along the length of the plant growing tank 3.
Example 4
The present embodiment is another modified scheme based on embodiment 2, and the main structure of the present embodiment is the same as that of embodiment 2, and the improvement point is that: as shown in fig. 2, a culture liquid tank 4 is arranged above the root system guide part 302, the culture liquid tank 4 is a U-shaped structure surrounded by the side wall of the vertical part 301, the top wall of the root system guide part 302 and the side wall of the upper chamber 103, the opening of the U-shaped structure is closed by a closing plate 401 to form a closed culture liquid tank 4, a plurality of liquid permeable holes 402 are distributed on the top wall of the root system guide part 302, the liquid permeable holes 402 drip the culture liquid in the culture liquid tank 4 into the root system guide part 302, and tend to preferentially diffuse close to the vertical part 301 under the action of gravity factors to guide the root system of the plant planted in the vertical part 301 to grow towards the root system guide part 302; a culture medium inlet 403 for introducing a culture medium into the culture medium tank 4 is provided on the surface of the sealing plate 401.
In this embodiment, the culture medium inlet 403 is normally closed, and the injected culture medium may be opened when necessary. Because the induction of culture solution, impel plant roots to violate the gravity characteristic, the slope upwards grows and enters into well cavity 104 along root system guide 302, and pours the culture solution that gets into in the root system guide 302, if appear when excessive, under the action of gravity, flows downwards along the diapire of root system guide 302 to discharge along flowing back bleeder vent 303.
Example 5
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 8, the secretion collecting column 203 is a bar formed by wet mixing a plurality of strands of bast fiber bundles 2032 and matrix soil 2033 arranged along the length direction of the bar, a layer of plastic film 2031 is wrapped outside the bar, non-woven fabric layers are wrapped at two ends of the bar to prevent the matrix soil 2033 from losing, and the matrix soil 2033 is formed by mixing vermiculite powder with the particle size not more than 1mm, river sand particles with the particle size not more than 2mm and peat soil in a mass ratio of 6:1: 3; the diameter of the bast fiber bundle 2032 is 1mm, the thickness of the plastic film 2031 is 0.1mm, the diameter of the secretion collecting column 203 is 1cm, and the length is 20-30 cm.
In this embodiment, the bast fiber bundles are formed by bundling bast fibers, such as flax fibers; the wet mixing of the bast fiber bundles 2032 and the matrix soil 2033 means that two circular disks with a plurality of circular holes correspondingly distributed on the surfaces are manufactured, and the two ends of the bast fiber bundles 2032 are respectively fixed by penetrating through the circular holes of the two circular disks; mixing and stirring matrix soil 2033 and distilled water to form wet soil, filling the wet soil into gaps of the bast fiber bundles 2032, finally wrapping the wet soil with a plastic film 2031 to form strips, and finally wrapping the two ends of the strips with non-woven fabrics.
Example 6
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 4, a culture solution input pipe 108 and a culture solution circulation injection pipe 109 are arranged at the top of the upper chamber 103, wherein the culture solution input pipe 108 is used for injecting culture solution for circulation into the upper chamber 103, and the culture solution circulation injection pipe 109 is connected with the peristaltic pump 6 through a hose.
Example 7
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 4, a gauze 1011 is disposed on the through slot 1010 of the middle chamber 104, and the gauze 1011 is used for preventing the cultivated soil in the root system guiding part 302 from entering the middle chamber 104, but allowing the root system to pass through the gauze 1011 to enter the middle chamber 104.
Example 8
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 4, the bottom wall of the lower chamber 105 is inclined from side to side, and a drain pipe 107 is provided at the lowest position; a filter cloth 106 is arranged below the bottom end of the secretion collecting column 203 in the lower chamber 105 to filter the culture solution seeped down from the secretion collecting column 203.
Example 9
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 5 and 6, a plurality of rows of mounting grooves 1012 are correspondingly formed on the upper separation plate 101 and the lower separation plate 102, each row of the secretion adsorption and collection unit 2 further comprises an upper mounting plate 201 and a lower mounting plate 202, the upper and lower ends of all secretion collection columns 203 in the row of secretion adsorption and collection unit 2 respectively penetrate through the upper mounting plate 201 and the lower mounting plate 202, the upper mounting plate 201 on each row of the secretion adsorption and collection unit 2 is fixed in the mounting groove 1012 of the upper separation plate 101, and the lower mounting plate 202 is fixed in the mounting groove 1012 of the lower separation plate 102; soil filling grooves 1013 are distributed at both ends of the installation groove 1012 of the upper partition plate 101, and root culture soil is filled in the middle chamber 104 through the soil filling grooves 1013.
Example 10
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 9, the secretion adsorption column 5 includes a vertical tube 501 with openings at two ends, two snap rings 502 are symmetrically arranged in the middle of the inner wall of the vertical tube 501, the two snap rings 502 are matched with plugs 507 arranged at two ends of the vertical tube 501 to fix a non-polar macroporous resin column 504 and a polar macroporous resin column 505 in the vertical tube 501 respectively, a liquid buffer region 503 is formed between the non-polar macroporous resin column 504 and the polar macroporous resin column 505, the plugs 507 are in threaded connection with the vertical tube 501, liquid pipes 508 are arranged in the middle of the plugs 507, two liquid pipes 508 at the top end and the bottom end of the vertical tube 501 are communicated with the peristaltic pump 6 and the lower chamber 105 through hoses respectively, and filter cotton 506 is arranged between the plugs 507 and the polar macroporous resin columns 505 at the bottom of the vertical tube 501.
Claims (10)
1. The utility model provides an absorption formula root system secretion collecting system, including plant cultivation groove (3), secretion collecting vat (1), secretion adsorption column (5) and peristaltic pump (6), wherein, plant cultivation groove (3) are connected with the lateral wall of secretion collecting vat (1), thereby make the plant roots who plants in plant cultivation groove (3) enter into in secretion collecting vat (1), through to after pouring into the culture solution culture in secretion collecting vat (1), start peristaltic pump (6), extract the culture solution in secretion collecting vat (1), make it flow back behind secretion adsorption column (5) and accomplish the circulation and collect its characterized in that to secretion collecting vat (1): the secretion collecting tank (1) is a closed tank body, an upper partition plate (101) and a lower partition plate (102) which are horizontally arranged in the secretion collecting tank separate an inner cavity of the secretion collecting tank into an upper cavity (103), a middle cavity (104) and a lower cavity (105) which are separated from each other, wherein the middle cavity (104) is communicated with the plant cultivation tank (3) through a through tank (1010) which is formed in the side wall of the middle cavity (104), so that a plant root system in the plant cultivation tank (3) enters the middle cavity (104) through the through tank (1010);
the middle chamber (104) is provided with a plurality of rows of secretion adsorption and collection units (2) in parallel, each row of root secretion adsorption and collection units (2) comprises a plurality of secretion collection columns (203) distributed along the height direction of the middle chamber (103), a gap (204) is formed between every two adjacent secretion collection columns (203), the upper end and the lower end of each secretion collection column (203) respectively penetrate through the upper partition plate (101) and the lower partition plate (102) and then extend into the upper chamber (103) and the lower chamber (105), and a culture solution channel for communicating the upper chamber (103) with the lower chamber (105) is formed; sterile river sand or sterile glass beads are filled in gaps between adjacent secretion adsorption and collection units (2) and between adjacent secretion collection columns (203) to form root system culture soil;
plant root systems in the middle chamber (104) can be pricked into the secretion collecting column (203) to grow or tightly attached to the secretion collecting column (203) to grow, so that root system secretions are attached to the surface of the secretion collecting column (203) or enter the secretion collecting column (203), culture solution injected into the upper chamber (103) can downwards move along the secretion collecting column (203) to enter the middle chamber (104), and then the plant root system secretions are carried to enter the lower chamber (105) along the secretion collecting column (203), and finally are adsorbed by the resin columns in the secretion adsorbing column (5) under the driving of the peristaltic pump (6).
2. The system for collecting root exudates according to claim 1, wherein: plant cultivation groove (3) are two cell bodies of symmetry setting in secretion collecting vat (1) both sides, and every cell body is including vertical portion (301) of planting the plant, and root system guide portion (302) and secretion collecting vat (1) intercommunication are passed through to the bottom of vertical portion (301).
3. The system for collecting root exudates according to claim 2, wherein: the bottom wall of the root system guide part (302) is obliquely arranged and gradually rises from the bottom of the vertical part (301) to one side of the secretion collecting tank (1); the lowest part of the vertical part (301) is provided with a liquid drainage air hole (303).
4. The system for collecting root exudates according to claim 2, wherein: the cultivation liquid tank (4) is arranged above the root system guide part (302), the cultivation liquid tank (4) is of a U-shaped structure formed by the side wall of the vertical part (301), the top wall of the root system guide part (302) and the side wall of the upper cavity (103) in an enclosing mode, the opening of the U-shaped structure is sealed by a sealing plate (401) to form the sealed cultivation liquid tank (4), a plurality of liquid penetrating holes (402) are distributed on the top wall of the root system guide part (302), the cultivation liquid in the cultivation liquid tank (4) is dripped into the root system guide part (302) through the liquid penetrating holes (402), and under the action of gravity factors, the cultivation liquid tends to be close to the vertical part (301) to preferentially diffuse so as to guide the root system of the plants planted in the vertical part (301) to grow towards the; a culture solution inlet (403) for pouring a culture solution into the culture solution tank (4) is provided on the surface of the sealing plate (401).
5. The system for collecting root exudates according to claim 1, wherein: the secretion collecting column (203) is a strip-shaped object formed by wet mixing a plurality of strands of bast fiber bundles (2032) and matrix soil (2033) which are arranged along the length direction of the secretion collecting column, a layer of plastic film (2031) is wrapped outside the strip-shaped object, two ends of the strip-shaped object are wrapped with non-woven fabric layers to prevent the matrix soil (2033) from losing, and the matrix soil (2033) is formed by mixing vermiculite powder with the grain diameter not more than 1mm, river sand particles with the grain diameter not more than 2mm and peat soil according to the mass ratio of 6:1: 3; the diameter of the bast fiber bundle (2032) is 1mm, the thickness of the plastic film (2031) is 0.1mm, the diameter of the secretion collecting column (203) is 1cm, and the length is 20-30 cm.
6. The system for collecting root exudates according to claim 1, wherein: the top of going up cavity (103) is provided with culture solution input tube (108) and culture solution circulation injection tube (109), and wherein, culture solution input tube (108) are used for going up into in cavity (103) and are used for the culture solution of circulation, culture solution circulation injection tube (109) are connected with peristaltic pump (6) through the hose.
7. The system for collecting root exudates according to claim 1, wherein: the middle chamber (104) is provided with gauze (1011) on running through groove (1010), and this gauze (1011) are used for preventing the cultivation soil in root system guide portion (302) from entering into middle chamber (104), but allow the root system to pass this gauze (1011) and get into middle chamber (104).
8. The system for collecting root exudates according to claim 1, wherein: the bottom wall of the lower chamber (105) is inclined from one side to the other side, and a liquid discharge pipe (107) is arranged at the lowest position; and a filter cloth (106) is arranged below the bottom end of the endocrine collection column (203) in the lower chamber (105) and used for filtering the culture solution seeped down by the endocrine collection column (203).
9. The system for collecting root exudates according to claim 1, wherein: the upper separation plate (101) and the lower separation plate (102) are correspondingly provided with a plurality of rows of mounting grooves (1012), each row of secretion adsorption and collection unit (2) also comprises an upper mounting plate (201) and a lower mounting plate (202), the upper end and the lower end of all secretion collection columns (203) in the secretion adsorption and collection unit (2) respectively penetrate through the upper mounting plate (201) and the lower mounting plate (202), the upper mounting plate (201) on each row of secretion adsorption and collection unit (2) is fixed in the mounting groove (1012) of the upper separation plate (101), and the lower mounting plate (202) is fixed in the mounting groove (1012) of the lower separation plate (102); soil filling grooves (1013) are distributed at two ends of the mounting groove (1012) on the upper partition plate (101), and root culture soil is filled in the middle chamber (104) through the soil filling grooves (1013).
10. The system for collecting root exudates according to claim 1, wherein: the secretion adsorption column (5) comprises a vertical tube body (501) with two open ends, two snap rings (502) are symmetrically arranged in the middle of the inner wall of the vertical pipe body (501), the two snap rings (502) are matched with plug bodies (507) arranged at two ends of the vertical pipe body (501) to respectively fix a non-polar macroporous resin column (504) and a polar macroporous resin column (505) in the vertical pipe body (501), and a liquid buffer area (503) is formed between the nonpolar macroporous resin column (504) and the polar macroporous resin column (505), the plug body (507) is in threaded connection with the vertical pipe body (501), and the middle part of the plug body (507) is provided with a liquid pipe (508), the two liquid pipes (508) at the top end and the bottom end of the vertical pipe body (501) are respectively communicated with the peristaltic pump (6) and the lower cavity chamber (105) through hoses, and filter cotton (506) is arranged between the plug body (507) at the bottom of the vertical pipe body (501) and the polar macroporous resin column (505).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011485298.1A CN112649241B (en) | 2020-12-16 | 2020-12-16 | Adsorption type root system secretion collecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011485298.1A CN112649241B (en) | 2020-12-16 | 2020-12-16 | Adsorption type root system secretion collecting system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112649241A true CN112649241A (en) | 2021-04-13 |
| CN112649241B CN112649241B (en) | 2023-05-26 |
Family
ID=75354238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011485298.1A Active CN112649241B (en) | 2020-12-16 | 2020-12-16 | Adsorption type root system secretion collecting system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112649241B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113804499A (en) * | 2021-08-26 | 2021-12-17 | 贵州大学 | Pinus massoniana rhizosphere secretion collecting device and collecting method thereof |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006262750A (en) * | 2005-03-23 | 2006-10-05 | Mirai:Kk | Three-dimensional hydroponics device |
| CN1963437A (en) * | 2006-11-10 | 2007-05-16 | 华南农业大学 | Collecting method and apparatus for secretion from root system of plant in pot |
| CN102636373A (en) * | 2012-05-07 | 2012-08-15 | 中国科学院南京土壤研究所 | Multi-channel plant root exudate collection system and collection method thereof |
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN204461820U (en) * | 2015-04-03 | 2015-07-08 | 上海市环境科学研究院 | A kind of movable-type secretions from plant roots classified collection device |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
-
2020
- 2020-12-16 CN CN202011485298.1A patent/CN112649241B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006262750A (en) * | 2005-03-23 | 2006-10-05 | Mirai:Kk | Three-dimensional hydroponics device |
| CN1963437A (en) * | 2006-11-10 | 2007-05-16 | 华南农业大学 | Collecting method and apparatus for secretion from root system of plant in pot |
| CN102636373A (en) * | 2012-05-07 | 2012-08-15 | 中国科学院南京土壤研究所 | Multi-channel plant root exudate collection system and collection method thereof |
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN204461820U (en) * | 2015-04-03 | 2015-07-08 | 上海市环境科学研究院 | A kind of movable-type secretions from plant roots classified collection device |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113804499A (en) * | 2021-08-26 | 2021-12-17 | 贵州大学 | Pinus massoniana rhizosphere secretion collecting device and collecting method thereof |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112649241B (en) | 2023-05-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112649241B (en) | Adsorption type root system secretion collecting system | |
| WO2019085393A1 (en) | Sewage treatment device, treatment method and treatment system | |
| CN103776662B (en) | A kind of xerophyte root exudates gathering-device and collection method thereof | |
| CN202083567U (en) | Device for collecting secretion of plant root | |
| CN112539969B (en) | Continuous collection device of plant root secretion | |
| CN105613213B (en) | Water seepage drip irrigation emitter | |
| CN103548660B (en) | Plant seedling pot allowing automatic liquid absorption | |
| CN209307244U (en) | A kind of solid-liquid separating equipment using biogas slurry production organic fertilizer | |
| CN109297871B (en) | Device and method for simulating pesticide migration and transformation process in paddy field water and soil system | |
| CN204443477U (en) | A kind of stereo drop-irrigation of control automatically device | |
| CN1137862C (en) | Domestic sewage treating system | |
| CN114112480B (en) | Pepper rhizosphere secretion collecting device and method | |
| CN203490112U (en) | Sampling and collecting device for soil leakage water of tobacco field | |
| CN110150038A (en) | A kind of fiber biological blanket with ecological purification function for sloping bank | |
| CN206442793U (en) | A kind of ecological Chinese prickly ash plantation of low stain | |
| CN214102560U (en) | Half root zone restriction cultivation structure of grape | |
| CN214750275U (en) | Simulated soil leaching device capable of sampling in layered mode | |
| CN211631226U (en) | Coconut husk strip cultivation device capable of draining water quickly | |
| CN113924956A (en) | Water-saving automatic infiltrating irrigation device | |
| CN209961502U (en) | Plant root system secretion collection device | |
| CN211793230U (en) | Big-arch shelter suitable for aquatic plant | |
| CN214696494U (en) | Roof greening circulating water device | |
| CN220211371U (en) | Radix tinosporae seedling raising bed | |
| CN214853039U (en) | Greening planting soil improvement structure | |
| CN214758401U (en) | Root-dividing region drip irrigation system for agricultural non-point source sewage purification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |