CN112649241B - Adsorption type root system secretion collecting system - Google Patents
Adsorption type root system secretion collecting system Download PDFInfo
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- CN112649241B CN112649241B CN202011485298.1A CN202011485298A CN112649241B CN 112649241 B CN112649241 B CN 112649241B CN 202011485298 A CN202011485298 A CN 202011485298A CN 112649241 B CN112649241 B CN 112649241B
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Abstract
An adsorption type root system secretion collecting system is characterized in that a plant cultivation groove is connected with the side wall of a secretion collecting groove, so that plant roots planted in the plant cultivation groove enter the secretion collecting groove, a peristaltic pump is started after culture fluid is poured into the secretion collecting groove for culture, the culture fluid in the secretion collecting groove is pumped, and flows back to the secretion collecting groove after passing through a secretion adsorption column to complete circulation collection, and a plurality of rows of secretion adsorption collecting units are arranged in a middle chamber of the secretion collecting groove in parallel; the invention utilizes the secretion adsorption and collection unit in the secretion collection tank to adsorb root secretion, and then utilizes the culture solution to dissolve and bring the root secretion adsorbed in the secretion adsorption and collection unit out of the secretion collection tank and then adsorb the root secretion by the resin column in the adsorption column, thereby not only simulating the natural environment of root growth, but also avoiding digging out the plant root system to damage the root system.
Description
Technical Field
The invention relates to the field of research of plant root exudates, in particular to an adsorption type root exudate collection system.
Background
The root system secretion refers to various substances secreted or released from different parts of the root system into the growth medium in the plant growth process, wherein the substances comprise organic substances with low relative molecular mass, mucilage substances with high molecular mass, root cell casts, decomposition products thereof, gas, protons, nutrient ions and the like. The root exudates have considerable quantity and great effect, are key factors for keeping the vigor of the rhizosphere microecological system, and are also important components of rhizosphere substance circulation. The root exudates obviously change the physical, chemical and biological properties of the root-soil interface, have important roles in the aspects of soil structure formation, soil nutrient activation, plant nutrient absorption, environmental stress relief and the like, and the research on the root exudates is an important content in the research fields of plant nutrition, chemical effect, biological pollution stress, environmental pollution repair and the like, and is widely paid attention to students at home and abroad.
In the prior art, the collection of root secretions mainly comprises a water culture method, a matrix culture collection method and a soil culture method. The hydroponic method does not need a special collecting device, but hydroponic plants generally have less root hair development and no mechanical resistance, and are greatly different from the aeration condition, nutrient distribution and root branch structure of the hydroponic plants grown under the actual soil culture condition. Therefore, the hydroponic method cannot obtain root secretions of the drought plants. The matrix culture method can ensure ventilation of the root system and has certain mechanical resistance, but has great difference with the root secretion in the natural state.
The traditional soil culture method is mainly used for collecting root secretions, firstly, after soil culture plants grow for a period of time, the root systems are taken out, rhizosphere soil is washed by distilled water, and the root secretions are obtained after shaking, centrifugation or filtration. And secondly, taking out the plants growing to a certain stage from the soil, washing the soil layer on the root system surface of the plants, and leaching the root system by using distilled water, wherein the leaching solution is used as root system secretion. During the whole operation process, the root system is extremely easy to damage, the collected solution contains a lot of contents of the root system and wounded liquid, and the method is generally only suitable for small plants or plants in seedling stage.
Disclosure of Invention
In order to solve the problems of the traditional soil culture method for collecting plant root system secretions, the invention provides an adsorption type root system secretion collecting system, the root system in the plant growth process is induced to intensively enter into a secretion collecting tank with a special structure, the secretion adsorbing and collecting unit in the secretion collecting tank is utilized to adsorb root system secretions, then the root system secretions adsorbed in the secretion adsorbing and collecting unit are dissolved and brought out of the secretion collecting tank by using a culture solution and then are adsorbed by a resin column in an adsorption column, so that the natural environment of root system growth can be simulated, the plant root system can be collected without digging out, the root system is prevented from being damaged, the collecting method is relatively simple and convenient, and a large amount of root system can be repeatedly collected in a period of time.
The technical scheme adopted by the invention for solving the technical problems is as follows: the utility model provides an absorption formula root system secretion collecting system, includes plant cultivation groove, secretion collecting vat, secretion adsorption column and peristaltic pump, wherein, plant cultivation groove is connected with the lateral wall of secretion collecting vat to make plant root system that plants in the plant cultivation groove enter into in the secretion collecting vat, after cultivateing through pouring culture solution into secretion collecting vat, start peristaltic pump, the culture solution in the extraction secretion collecting vat makes it flow back to secretion collecting vat after the secretion adsorption column and accomplish the circulation collection, secretion collecting vat is a confined cell body, and upper division board and the lower division board that its interior level was provided with separate into each other upper chamber, well chamber and lower chamber, wherein, well chamber is through the through-groove intercommunication that sets up on its lateral wall with plant cultivation groove, so that plant root system in the plant cultivation groove enters into in the well chamber through this through-groove;
the middle chamber is provided with a plurality of rows of secretion adsorption and collection units in parallel, each row of root secretion adsorption and collection units comprises a plurality of secretion collection columns distributed along the height direction of the middle chamber, a gap is reserved between every two adjacent secretion collection columns, and the upper end and the lower end of each secretion collection column respectively penetrate through the upper partition plate and the lower partition plate and then extend into the upper chamber and the lower chamber to form a culture solution channel communicated with the upper chamber and the lower chamber; sterile river sand or sterile glass beads are filled in gaps between adjacent secretion adsorption collecting units and gaps between adjacent secretion collecting columns to form root system culture soil;
plant roots in the middle chamber can be pricked into the secretion collecting column to grow or cling to the secretion collecting column to grow in the growth process, so that root system secretion is attached to the surface of the secretion collecting column or enters the inside of the secretion collecting column, culture solution injected into the upper chamber can move downwards along the secretion collecting column to enter the middle chamber, further plant root system secretion is carried along the secretion collecting column to enter the lower chamber, and finally the plant root system secretion is adsorbed by the resin column in the secretion collecting column after passing through the secretion adsorbing column under the driving of the peristaltic pump.
As a preferred scheme of the adsorption type root secretion collecting system, the plant cultivation groove is two groove bodies symmetrically arranged on two sides of the secretion collecting groove, each groove body comprises a vertical portion for planting plants, and the bottom of the vertical portion is communicated with the secretion collecting groove through a root guiding portion.
As another preferable mode of the adsorption type root exudate collection system, the bottom wall of the root guiding part is arranged obliquely, and gradually rises from the bottom of the vertical part to one side of the exudate collection tank; the lowest part of the vertical part is provided with a liquid draining vent hole.
As another preferable scheme of the adsorption type root secretion collecting system, a culture solution tank is arranged above the root system guiding part, the culture solution tank is of a U-shaped structure formed by enclosing a side wall of the vertical part, a top wall of the root system guiding part and a side wall of the upper cavity, openings of the U-shaped structure are sealed by sealing plates to form a sealed culture solution tank, a plurality of liquid-permeable holes are distributed on the top wall of the root system guiding part, the liquid-permeable holes drip the culture solution in the culture solution tank into the root system guiding part, and the culture solution tends to preferentially diffuse close to the vertical part under the action of gravity factors so as to guide the root system planted in the vertical part to grow towards the root system guiding part; the surface of the sealing plate is provided with a culture solution injection port for injecting culture solution into the culture solution tank.
As another preferable scheme of the adsorption type root secretion collecting system, the secretion collecting column is a strip-shaped object formed by wet mixing a plurality of phloem fiber bundles arranged along the length direction of the secretion collecting column and matrix soil, a layer of plastic film is wrapped outside the strip-shaped object, two ends of the strip-shaped object are wrapped with non-woven fabrics layers so as to prevent matrix soil loss, and the matrix soil is formed by mixing vermiculite powder with the grain diameter of not more than 1mm, river sand particles with the grain diameter of not more than 2mm and peat soil according to the mass ratio of 6:1:3; the diameter of the bast fiber bundle is 1mm, the thickness of the plastic film is 0.1mm, the diameter of the secretion collecting column is 1cm, and the length is 20-30cm.
As another preferable scheme of the adsorption type root secretion collecting system, a culture solution input pipe and a culture solution circulating injection pipe are arranged at the top of the upper chamber, wherein the culture solution input pipe is used for injecting culture solution for circulation into the upper chamber, and the culture solution circulating injection pipe is connected with a peristaltic pump through a hose.
As another preferable scheme of the adsorption type root exudate collection system, a gauze is arranged on the through groove of the middle chamber, and the gauze is used for preventing the cultivation soil in the root system guiding part from entering the middle chamber, but allowing the root system to pass through the gauze and enter the middle chamber.
As another preferable scheme of the adsorption type root exudate collection system, the bottom wall of the lower chamber is inclined from one side to the other side, and a drain pipe is arranged at the lowest position; a filter cloth is arranged below the bottom end of the secretion collecting column of the lower chamber, and the culture solution permeated by the secretion collecting column is filtered.
As another preferable scheme of the adsorption type root system secretion collecting system, the upper partition plate and the lower partition plate are correspondingly provided with a plurality of rows of mounting grooves, each row of secretion adsorption collecting units further comprises an upper mounting plate and a lower mounting plate, the upper and lower ends of all secretion collecting columns in the row of secretion adsorption collecting units respectively penetrate through the upper mounting plate and the lower mounting plate, the upper mounting plate on each row of secretion adsorption collecting units is fixed in the mounting groove of the upper partition plate, and the lower mounting plate is fixed in the mounting groove of the lower partition plate; soil filling grooves are distributed at two ends of the mounting groove on the upper partition plate, and root system culture soil is filled into the middle cavity through the soil filling grooves.
As another preferred scheme of above-mentioned absorption formula root system secretion collecting system, the secretion adsorption column includes a both ends open-ended vertical body, is provided with two snap rings at vertical body inner wall's middle part symmetry, and these two snap rings are fixed nonpolar macroporous resin column and polarity macroporous resin column in vertical body respectively with the cock body cooperation that vertical body both ends set up, and form the liquid buffer between nonpolar macroporous resin column and polarity macroporous resin column, cock body and vertical body threaded connection, and the middle part of cock body all is provided with the liquid pipe, and two liquid pipes of vertical body top and bottom are communicated with peristaltic pump and lower cavity respectively through the hose, and are provided with the filter pulp between cock body and the polarity macroporous resin column of vertical body bottom.
Compared with the prior art, the invention has the following beneficial effects:
1) According to the invention, root systems in the growth process of the plants are induced to enter the secretion collecting tank with a special structure in a concentrated manner, the secretion in the secretion collecting tank is utilized to absorb the root system secretion, then the root system secretion absorbed in the secretion absorbing and collecting unit is dissolved and brought out of the secretion collecting tank by using the culture solution and then is absorbed by the resin column in the absorption column, so that the natural environment of root system growth can be simulated, the plant root systems can be collected without digging out, the damage to the root systems is avoided, and the collecting method is relatively simple and convenient and can be used for repeatedly collecting a large amount of root systems in a period of time;
2) The secretion adsorption and collection unit in the cavity divides the whole cavity into a plurality of gaps by utilizing a plurality of secretion collection columns, most of the space of the whole cavity is occupied by the secretion collection columns after the gaps are filled with root culture soil, the root system inevitably pierces the secretion collection columns to grow in the culture soil in the growth process, so that generated secretion can be dissolved in culture solution, enters the lower cavity along with the secretion collection columns and is finally adsorbed by the resin columns, the collection process is completed, and the collection process is simple and convenient;
3) The plant cultivation groove is divided into the vertical part and the root system guide part, the root system guide part is inclined upwards, the culture solution is filled into the root system guide part by utilizing the culture solution groove to promote the growth and development of the root system, the root system is guided to enter the middle cavity for growth, the inclined upwards structure can enable the root system to grow against the gravity factor, the gathering effect of the root system is achieved, the root system grows more densely, the collection efficiency is improved, and the redundant culture solution does not enter the middle cavity, so that the growth condition of the root system secretion to be collected is changed;
4) The main body of the secretion collecting column is a strip-shaped object formed by wet mixing bast fiber bundles and matrix soil, wherein the matrix soil is formed by vermiculite powder, river sand and peat soil, the bast fiber bundles provide culture solution water diversion channels, the bast fiber bundles and the plastic film wrapped outside form a culture solution conveying channel in a matching way, in the plant root system growing process, the plastic film is punctured to enter the culture solution conveying channel for growth, and secretion of the secretion is naturally taken away by the culture solution and is adsorbed by the resin column; in order to prevent matrix soil from being taken away by the culture solution, the two ends of the strip-shaped object are wrapped by non-woven fabrics, and fiber holes of the non-woven fabrics can play a good role in blocking and filtering, so that the loss of the matrix soil is prevented, but the circulation of the culture solution is not prevented.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention in use;
FIG. 2 is a schematic diagram of the structure of the present invention;
FIG. 3 is a schematic top view of FIG. 2;
FIG. 4 is a schematic structural view of a secretion collecting tank;
fig. 5 is a schematic structural view of the upper partition plate;
fig. 6 is a schematic structural view of the bottom partition plate;
FIG. 7 is a schematic structural view of a secretion adsorbing and collecting unit;
FIG. 8 is a schematic structural view of a secretion collection column;
FIG. 9 is a schematic structural view of a secretion adsorbing column;
reference numerals: 1. secretion collecting tank, 101, upper partition plate, 102, lower partition plate, 103, upper chamber, 104, middle chamber, 105, lower chamber, 106, filter cloth, 107, drain, 108, culture fluid input pipe, 109, culture fluid circulation injection pipe, 1010, through tank, 1011, gauze, 1012, mounting tank, 1013, soil filling tank, 2, secretion adsorption collecting unit, 201, upper mounting plate, 202, lower mounting plate, 203, secretion collecting column, 2031, plastic film, 2032, phloem fiber bundle, 2033, matrix soil, 204, gap, 3, plant cultivation tank, 301, vertical part, 302, root guide, 303, drain vent, 4, culture fluid tank, 401, sealing plate, 402, liquid permeation hole, 403, culture fluid injection port, 5, secretion adsorption column, 501, vertical pipe, 502, clasp, 503, liquid buffer zone, 504, nonpolar macroporous resin column, 505, polar macroporous resin column, 506, filter cotton, 507, plug, 508, liquid pipe, 6, peristaltic pump.
Detailed Description
The technical scheme of the invention is further elaborated in the following in conjunction with specific embodiments. The plants planted in the plant cultivation tank can be cultivated according to the research type, and the soil in the plant cultivation tank and the used culture solution are determined according to the planted plants.
Example 1
As shown in fig. 1-4 and 7, an adsorption type root system secretion collecting system comprises a plant cultivating tank 3, a secretion collecting tank 1, a secretion adsorbing column 5 and a peristaltic pump 6, wherein the plant cultivating tank 3 is connected with the side wall of the secretion collecting tank 1, so that plant roots planted in the plant cultivating tank 3 enter the secretion collecting tank 1, after the culture solution is poured into the secretion collecting tank 1 for cultivation, the peristaltic pump 6 is started, the culture solution in the secretion collecting tank 1 is pumped, and the culture solution flows back to the secretion collecting tank 1 after passing through the secretion adsorbing column 5 to complete circulation collection, the secretion collecting tank 1 is a closed tank body, an upper partition plate 101 and a lower partition plate 102 which are horizontally arranged in the secretion collecting tank are used for dividing the internal cavity of the secretion collecting tank into an upper cavity 103, a middle cavity 104 and a lower cavity 105 which are separated from each other, and the middle cavity 104 is communicated with the plant cultivating tank 3 through a through groove 1010 formed in the side wall of the plant cultivating tank 3, so that the plant roots in the plant cultivating tank 3 enter the middle cavity 104 through the through groove 1010;
the middle chamber 104 is provided with a plurality of rows of secretion adsorption and collection units 2 in parallel, each row of root secretion adsorption and collection units 2 comprises a plurality of secretion collection columns 203 distributed along the height direction of the middle chamber 103, gaps 204 are reserved between every two adjacent secretion collection columns 203, and the upper end and the lower end of each secretion collection column 203 respectively penetrate through the upper partition plate 101 and the lower partition plate 102 and then extend into the upper chamber 103 and the lower chamber 105 to form a culture solution channel communicated with the upper chamber 103 and the lower chamber 105; gaps between adjacent secretion adsorption collecting units 2 and between adjacent secretion collecting columns 203 are filled with sterile river sand or sterile glass beads to form root system culture soil;
plant root systems in the middle chamber 104 can be pricked into the secretion collecting column 203 to grow or cling to the secretion collecting column 203 to grow in the growth process, so that root system secretions are attached to the surface of the secretion collecting column 203 or enter the inside of the secretion collecting column 203, culture fluid injected into the upper chamber 103 can move downwards along the secretion collecting column 203 to enter the middle chamber 104, and then plant root system secretions are carried along the secretion collecting column 203 to enter the lower chamber 105, and finally are adsorbed by resin columns in the secretion adsorbing column under the driving of the peristaltic pump 6 after passing through the secretion adsorbing column 5.
In the implementation of the invention, the plant cultivation tank 3 and the secretion collecting tank 1 can be arranged in a long shape, and then one or more rows of plants are planted in the plant cultivation tank 3 in an array manner, so that root systems of the plants can be collected in the secretion collecting tank 1, and the secretion collecting amount is improved; the secretion adsorbing and collecting units 2 in the middle chamber 104 are distributed along the length direction, the width of a gap 204 between two adjacent secretion collecting columns 203 is generally 1cm, and the distance between two adjacent rows of secretion adsorbing and collecting units 2 is also 1cm; finally, root system culture soil is filled between the secretion collecting columns 203, and root systems of plants inevitably root in the secretion collecting columns 203 in the growth process;
in the invention, the plant cultivation tank 3 and the secretion collecting tank 1 are both made of transparent organic glass, so that the growth distribution condition of the plant root system can be conveniently observed, and when a large amount of plant root systems are observed to enter the secretion collecting tank 1 for growth (the root systems are distributed in at least half of the volume of the middle chamber 104), the root system secretion is collected, and the operation steps are as follows:
1) Injecting culture solution into the upper chamber 103 through the culture solution input pipe 108, allowing the culture solution in the upper chamber 103 to permeate into the lower chamber 105 and the middle chamber 104 through the secretion collecting column 203, observing the lower chamber 105 until the culture solution fills the lower chamber 105, stopping injecting the culture solution into the upper chamber 103, and standing for 5-8h;
2) Starting a peristaltic pump 6 to pump the culture solution in the lower chamber 105, enabling the culture solution to pass through the secretion adsorption column 5, and then injecting the culture solution into the upper chamber 103 through a culture solution circulation injection pipe 109 to complete one circulation, wherein root secretions are adsorbed by a resin column in the secretion adsorption column 5 in the circulation process;
3) The root system secretion collection is completed after the circulation for a plurality of times, and the root system secretion can be obtained by taking down the resin column in the secretion adsorption column 5 and performing the conventional elution operation;
it should be noted that: the components of the culture solution, the operation parameters of the peristaltic pump 6, the selection of the resin column, the subsequent elution of the resin column, etc. are all those skilled in the art and can be selected and used according to the actual situation, and will not be described herein.
The above is a basic embodiment of the present invention, and further improvements, optimization and limitation can be made on the above basis, so as to obtain the following examples:
example 2
The embodiment is an improvement scheme based on embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 1-3, the plant cultivation tank 3 is two tanks symmetrically arranged at two sides of the secretion collecting tank 1, each tank comprises a vertical portion 301 for planting plants, and the bottom of the vertical portion 301 is communicated with the secretion collecting tank 1 through a root system guiding portion 302.
Example 3
The embodiment is an improvement scheme based on embodiment 2, and the main structure of the embodiment is the same as that of embodiment 2, and the improvement points are that: as shown in fig. 2, the bottom wall of the root guide 302 is inclined, and gradually rises from the bottom of the vertical portion 301 to one side of the secretion collecting tank 1; a drain vent 303 is provided at the lowest position of the vertical portion 301.
In this embodiment, the drain holes 303 may be provided at intervals along the length direction of the plant-growing tank 3.
Example 4
The embodiment is another improvement on the basis of embodiment 2, and the main structure of the embodiment is the same as that of embodiment 2, and the improvement points are that: as shown in fig. 2, a culture solution tank 4 is disposed above the root system guiding portion 302, the culture solution tank 4 is a U-shaped structure surrounded by a side wall of the vertical portion 301, a top wall of the root system guiding portion 302 and a side wall of the upper chamber 103, an opening of the U-shaped structure is closed by a sealing plate 401 to form a closed culture solution tank 4, a plurality of liquid-permeable holes 402 are distributed on the top wall of the root system guiding portion 302, the liquid-permeable holes 402 drip the culture solution in the culture solution tank 4 into the root system guiding portion 302, and under the action of gravity, the culture solution tends to preferentially diffuse close to the vertical portion 301 so as to guide the root system of the plant planted in the vertical portion 301 to grow towards the root system guiding portion 302; a culture medium inlet 403 for filling the culture medium into the culture medium tank 4 is provided on the surface of the sealing plate 401.
In this embodiment, the culture medium injection port 403 is normally closed, and the injection of the culture medium may be opened when necessary. Due to the induction of the culture solution, the plant root system is caused to grow obliquely upwards along the root system guide part 302 and enter the middle chamber 104 against the gravity characteristic, and if excessive culture solution is poured into the root system guide part 302, the culture solution flows downwards along the bottom wall of the root system guide part 302 and is discharged along the liquid discharge and ventilation holes 303 under the action of gravity.
Example 5
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 8, the secretion collecting column 203 is a strip formed by wet mixing a plurality of bast fiber bundles 2032 and matrix soil 2033, a layer of plastic film 2031 is wrapped outside the strip, two ends of the strip are wrapped with non-woven fabrics to prevent the matrix soil 2033 from running off, and the matrix soil 2033 is formed by mixing vermiculite powder with a particle size of not more than 1mm, river sand particles with a particle size of not more than 2mm and peat soil in a mass ratio of 6:1:3; the bast fiber bundle 2032 has a diameter of 1mm, the plastic film 2031 has a thickness of 0.1mm, the secretion collecting column 203 has a diameter of 1cm, and the length of 20-30cm.
In this embodiment, the bast fiber bundles are bast fibers, such as flax fibers, bound together; wet mixing of the bast fiber bundle 2032 and the matrix soil 2033 means that two discs with a plurality of round holes distributed on the surfaces are manufactured, and two ends of the bast fiber bundle 2032 respectively penetrate through the round holes of the two discs to be fixed; mixing matrix soil 2033 with distilled water, stirring to form wet soil, filling the wet soil into gaps of bast fiber bundles 2032, wrapping with plastic film 2031 to form strips, and wrapping with non-woven fabrics at both ends of the strips.
Example 6
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 4, a culture fluid input pipe 108 and a culture fluid circulation injection pipe 109 are arranged at the top of the upper chamber 103, wherein the culture fluid input pipe 108 is used for injecting culture fluid for circulation into the upper chamber 103, and the culture fluid circulation injection pipe 109 is connected with the peristaltic pump 6 through a hose.
Example 7
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 4, a gauze 1011 is disposed on the through slot 1010 of the middle chamber 104, and the gauze 1011 is used to prevent the cultivation soil in the root guide 302 from entering the middle chamber 104, but allows the root to pass through the gauze 1011 and enter the middle chamber 104.
Example 8
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 4, the bottom wall of the lower chamber 105 is inclined from one side to the other side, and a drain pipe 107 is provided at the lowest position; a filter cloth 106 is provided below the bottom end of the secretion collecting column 203 in the lower chamber 105, and the culture solution permeated by the secretion collecting column 203 is filtered.
Example 9
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 5 and 6, the upper partition plate 101 and the lower partition plate 102 are correspondingly provided with a plurality of rows of mounting grooves 1012, each row of the secretion adsorbing and collecting unit 2 further comprises an upper mounting plate 201 and a lower mounting plate 202, the upper and lower ends of all secretion collecting columns 203 in the row of secretion adsorbing and collecting units 2 respectively penetrate through the upper mounting plate 201 and the lower mounting plate 202, and the upper mounting plate 201 on each row of secretion adsorbing and collecting units 2 is fixed in the mounting groove 1012 of the upper partition plate 101, and the lower mounting plate 202 is fixed in the mounting groove 1012 of the lower partition plate 102; soil filling grooves 1013 are distributed at both ends of the mounting groove 1012 on the upper partition plate 101, and root culture soil is filled into the middle chamber 104 through the soil filling grooves 1013.
Example 10
The embodiment is another improvement on the basis of embodiment 1, and the main structure of the embodiment is the same as that of embodiment 1, and the improvement points are that: as shown in fig. 9, the secretion adsorbing column 5 comprises a vertical tube body 501 with two open ends, two clamping rings 502 are symmetrically arranged in the middle of the inner wall of the vertical tube body 501, the two clamping rings 502 are matched with plug bodies 507 arranged at the two ends of the vertical tube body 501 to fix a nonpolar macroporous resin column 504 and a polar macroporous resin column 505 in the vertical tube body 501 respectively, a liquid buffer area 503 is formed between the nonpolar macroporous resin column 504 and the polar macroporous resin column 505, the plug bodies 507 are in threaded connection with the vertical tube body 501, liquid pipes 508 are respectively arranged in the middle of the plug bodies 507, the two liquid pipes 508 at the top end and the bottom end of the vertical tube body 501 are respectively communicated with the peristaltic pump 6 and the lower cavity 105 through hoses, and filter cotton 506 is arranged between the plug bodies 507 at the bottom of the vertical tube body 501 and the polar macroporous resin column 505.
Claims (7)
1. The utility model provides an absorption formula root system secretion collecting system, including plant cultivation groove (3), secretion collecting vat (1), secretion adsorption column (5) and peristaltic pump (6), wherein, plant cultivation groove (3) are connected with the lateral wall of secretion collecting vat (1), thereby make plant root system that plants in plant cultivation groove (3) enter into in secretion collecting vat (1), after cultivateing through filling culture solution into secretion collecting vat (1), start peristaltic pump (6), extract the culture solution in secretion collecting vat (1), make it flow back to secretion collecting vat (1) after secretion adsorption column (5) accomplish circulation collection, its characterized in that: the secretion collecting tank (1) is a closed tank body, an upper partition plate (101) and a lower partition plate (102) which are horizontally arranged in the tank body divide an internal cavity of the tank body into an upper cavity (103), a middle cavity (104) and a lower cavity (105) which are mutually separated, wherein the middle cavity (104) is communicated with the plant cultivating tank (3) through a penetrating groove (1010) formed in the side wall of the middle cavity (104), so that plant roots in the plant cultivating tank (3) enter the middle cavity (104) through the penetrating groove (1010);
the middle chamber (104) is provided with a plurality of rows of secretion adsorption and collection units (2) in parallel, each row of root secretion adsorption and collection unit (2) comprises a plurality of secretion collection columns (203) distributed along the height direction of the middle chamber (103), gaps (204) are formed between every two adjacent secretion collection columns (203), and the upper end and the lower end of each secretion collection column (203) respectively penetrate through the upper partition plate (101) and the lower partition plate (102) and then extend into the upper chamber (103) and the lower chamber (105) to form a culture solution channel communicated with the upper chamber (103) and the lower chamber (105); sterile river sand or sterile glass beads are filled in gaps between adjacent secretion adsorption and collection units (2) and between adjacent secretion collection columns (203) to form root system culture soil;
the plant root system in the middle chamber (104) can be pricked into the secretion collecting column (203) to grow or cling to the secretion collecting column (203) to grow in the growth process, so that root system secretion is attached to the surface of the secretion collecting column (203) or enters the inside of the secretion collecting column (203), the culture solution injected into the upper chamber (103) can move downwards along the secretion collecting column (203) into the middle chamber (104), and then the plant root system secretion is carried along the secretion collecting column (203) and then enters the lower chamber (105), and finally is adsorbed by the resin column in the secretion collecting column after passing through the secretion adsorbing column (5) under the driving of the peristaltic pump (6);
a plurality of rows of mounting grooves (1012) are correspondingly formed in the upper partition plate (101) and the lower partition plate (102), each row of secretion adsorption and collection units (2) further comprises an upper mounting plate (201) and a lower mounting plate (202), the upper ends and the lower ends of all secretion collection columns (203) in the secretion adsorption and collection units (2) penetrate through the upper mounting plate (201) and the lower mounting plate (202) respectively, the upper mounting plate (201) on each row of secretion adsorption and collection units (2) is fixed in the mounting groove (1012) of the upper partition plate (101), and the lower mounting plate (202) is fixed in the mounting groove (1012) of the lower partition plate (102); soil filling grooves (1013) are distributed at two ends of an installation groove (1012) on the upper partition plate (101), and root culture soil is filled into the middle chamber (104) through the soil filling grooves (1013);
the plant cultivation groove (3) is two groove bodies symmetrically arranged on two sides of the secretion collection groove (1), each groove body comprises a vertical part (301) for planting plants, and the bottom of the vertical part (301) is communicated with the secretion collection groove (1) through a root system guide part (302);
secretion adsorption column (5) include a both ends open-ended vertical body (501), are provided with two snap rings (502) at the middle part symmetry of vertical body (501) inner wall, and these two snap rings (502) cooperate with stopper body (507) that vertical body (501) both ends set up respectively with nonpolar macroporous resin post (504) and polarity macroporous resin post (505) fix in vertical body (501), and form liquid buffer (503) between nonpolar macroporous resin post (504) and polarity macroporous resin post (505), stopper body (507) and vertical body (501) threaded connection, and the middle part of stopper body (507) all is provided with liquid pipe (508), and two liquid pipes (508) of vertical body (501) top and bottom are communicated with peristaltic pump (6) and lower cavity (105) respectively through the hose, and are provided with between stopper body (507) and polarity macroporous resin post (505) of vertical body (501) bottom and filter cotton (506).
2. The system for collecting root system secretions of claim 1 wherein: the bottom wall of the root system guiding part (302) is obliquely arranged, and gradually rises from the bottom of the vertical part (301) to one side of the secretion collecting tank (1); a drain vent (303) is arranged at the lowest part of the vertical part (301).
3. The system for collecting root system secretions of claim 1 wherein: a culture solution tank (4) is arranged above the root system guide part (302), the culture solution tank (4) is of a U-shaped structure formed by enclosing the side wall of the vertical part (301), the top wall of the root system guide part (302) and the side wall of the upper chamber (103), an opening of the U-shaped structure is sealed by a sealing plate (401) to form a sealed culture solution tank (4), a plurality of liquid-permeable holes (402) are distributed on the top wall of the root system guide part (302), the liquid-permeable holes (402) drop the culture solution in the culture solution tank (4) into the root system guide part (302), and under the action of gravity, the culture solution tends to preferentially diffuse close to the vertical part (301) so as to guide the root system of the plant planted in the vertical part (301) to grow towards the root system guide part (302); a culture medium injection port (403) for injecting a culture medium into the culture medium tank (4) is provided on the surface of the sealing plate (401).
4. The system for collecting root system secretions of claim 1 wherein: the secretion collecting column (203) is a strip-shaped object formed by wet mixing a plurality of phloem fiber bundles (2032) arranged along the length direction of the secretion collecting column and matrix soil (2033), a layer of plastic film (2031) is wrapped outside the strip-shaped object, two ends of the strip-shaped object are wrapped with non-woven fabric layers so as to prevent the matrix soil (2033) from losing, and the matrix soil (2033) is formed by mixing vermiculite powder with the grain diameter not exceeding 1mm, river sand grains with the grain diameter not exceeding 2mm and peat soil in a mass ratio of 6:1:3; the diameter of the bast fiber bundle (2032) is 1mm, the thickness of the plastic film (2031) is 0.1mm, the diameter of the secretion collecting column (203) is 1cm, and the length is 20-30cm.
5. The system for collecting root system secretions of claim 1 wherein: the top of the upper chamber (103) is provided with a culture solution input pipe (108) and a culture solution circulation injection pipe (109), wherein the culture solution input pipe (108) is used for injecting culture solution for circulation into the upper chamber (103), and the culture solution circulation injection pipe (109) is connected with a peristaltic pump (6) through a hose.
6. The system for collecting root system secretions of claim 1 wherein: a gauze (1011) is arranged on the penetrating groove (1010) of the middle chamber (104), the gauze (1011) is used for preventing the cultivation soil in the root system guiding part (302) from entering the middle chamber (104), but allowing the root system to pass through the gauze (1011) and enter the middle chamber (104).
7. The system for collecting root system secretions of claim 1 wherein: the bottom wall of the lower chamber (105) is inclined from one side to the other side, and a liquid discharge pipe (107) is arranged at the lowest position; a filter cloth (106) is arranged below the bottom end of the endocrine collection column (203) of the lower chamber (105), and the culture solution permeated by the endocrine collection column (203) is filtered.
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