CN112640728A - Industrialized bottle cultivation method for seafood mushrooms - Google Patents
Industrialized bottle cultivation method for seafood mushrooms Download PDFInfo
- Publication number
- CN112640728A CN112640728A CN202110033407.4A CN202110033407A CN112640728A CN 112640728 A CN112640728 A CN 112640728A CN 202110033407 A CN202110033407 A CN 202110033407A CN 112640728 A CN112640728 A CN 112640728A
- Authority
- CN
- China
- Prior art keywords
- culture
- culture medium
- bottle
- materials
- stock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 23
- 235000014102 seafood Nutrition 0.000 title claims abstract description 14
- 238000012364 cultivation method Methods 0.000 title claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 37
- 241001534815 Hypsizygus marmoreus Species 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 11
- 235000009566 rice Nutrition 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- 240000006394 Sorghum bicolor Species 0.000 claims abstract description 7
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims abstract description 7
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 24
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 12
- 239000001569 carbon dioxide Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 9
- 230000007246 mechanism Effects 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 239000008235 industrial water Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 239000004745 nonwoven fabric Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 239000012257 stirred material Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 3
- 239000001052 yellow pigment Substances 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000002361 compost Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000726811 Carpinus betulus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a seafood mushroom factory bottle cultivation method, which comprises the following steps: firstly, preparing materials; preparing a mother culture medium and a stock culture medium, wherein the mother culture medium is a PDA culture medium; the stock culture medium is dry sawdust, rice bran and wheat bran; secondly, preparing a culture material; preparing a stock culture medium and a stock culture medium, proportionally mixing dry sawdust, rice bran and wheat bran in the stock culture medium with sorghum and cottonseed hull materials and the stock culture medium, standing, putting into a stirrer for stirring after sufficient water absorption, and stirring to obtain the culture material. The industrial bottle cultivation of the hypsizygus marmoreus adopts advanced industrialized equipment and technology, realizes the automatic, standardized and large-scale production of the hypsizygus marmoreus, and the compost can be obtained locally, thereby greatly saving the transportation cost and the purchase cost, reducing the production cost of the hypsizygus marmoreus and improving the comprehensive economic value of the industrialized production of the hypsizygus marmoreus.
Description
Technical Field
The invention relates to the technical field of industrial cultivation, in particular to a method for cultivating seafood mushroom in bottles in an industrialized mode.
Background
Hypsizygus marmoreus, also known as Hypsizygus marmoreus, etc., is a rare fungus used as both medicine and food, which has been successfully domesticated in recent years, and is named because the mushroom has seafood flavor. Although the seafood mushrooms are similar to white beech mushrooms in naming classification, the commercial mushrooms are characterized by being slightly long in stipe, fleshy, delicious in taste, rich in protein, various mineral substances and vitamins, capable of reducing blood pressure and cholesterol and having auxiliary effects of resisting cancer and the like after being eaten for a long time, known as 'king of mushrooms', long in fresh-keeping period of up to 1 month, suitable for distant marketing, unavailable in domestic and foreign markets and quite wide in prospect.
At present, the production of the hypsizygus marmoreus mainly utilizes natural climate, the modernization degree is low, the yield and the quality are not guaranteed, and the industrial bottle cultivation method for the hypsizygus marmoreus is provided for improving the agricultural development level, improving the quality of agricultural products and accelerating the production yield of the hypsizygus marmoreus.
Disclosure of Invention
In order to achieve the purpose, the invention provides the following technical scheme: an industrial bottle cultivation method for seafood mushrooms comprises the following operation steps:
preparation of materials
Preparing a mother culture medium and a stock culture medium, wherein the mother culture medium is a PDA culture medium; the stock culture medium is dry sawdust, rice bran and wheat bran;
secondly, preparation of culture material
Preparing a stock culture medium and a mother culture medium, controlling the water content in the stock culture medium to be 60-65% and the pH value to be 7.2-7.5, mixing dry sawdust, rice bran and wheat bran in the mother culture medium with sorghum and cottonseed hull materials in proportion with the stock culture medium, standing, putting into a stirrer for stirring after fully absorbing water, and obtaining a culture material after stirring;
thirdly, planting in a bottle
Sterilizing the culture materials, cooling, feeding the corresponding culture materials into an aseptic machine room for aseptic operation, bottling the culture materials by automatic mechanical equipment, and filling the corresponding stirred materials into a culture bottle;
cultivation of sea fresh mushroom
Firstly, feeding bottled culture materials into an automatic inoculation machine for strain inoculation treatment, feeding the culture materials into a culture chamber by a conveying mechanism after inoculation is finished, and reasonably culturing by controlling a proper temperature; culturing for a certain number of days under the conditions of proper temperature, humidity and carbon dioxide concentration, and continuing to perform after-ripening culture for a certain number of days after the mycelia reach the bottom of the bottle, wherein the mycelia are sparse and turn into thick white to form thick and strong mycelia, the material surface is white and granular, and light yellow pigment is secreted, so that physiological maturity is achieved;
fifth, fruiting and harvesting of Hypsizygus marmoreus
After the strain grows over the bottle, entering a fruiting management stage, performing bottle opening and mycelium stimulation measures for conventional operation, injecting 10-15ml of water into the bottle through an industrial water injection mechanism, paving non-woven fabrics at the bottle mouth of the culture bottle, and controlling the culture temperature and humidity; and when mushroom buds appear, removing the covered compound bars, controlling the culture temperature, spraying atomized water into the culture room by a water spraying mechanism, keeping the indoor humidity, carrying out normal ventilation treatment, controlling the concentration of carbon dioxide, waiting for a certain number of days, and picking the hypsizygus marmoreus by picking equipment.
Preferably, the composition ratio of the dry wood chips, the rice bran and the bran in the stock culture medium is 54%: 33.4%: 12.6 percent.
Preferably, the selection of the culture flask can be between 500 and 1000 ml.
Preferably, the temperature of the cultivation culture is controlled between 20 ℃ and 22 ℃, the humidity is controlled at 65%, and the carbon dioxide concentration is controlled at 3000-4000mg/L-1The number of days of early culture is about 50-55 days, and the number of days of late physiological maturation culture is 30-35 days.
Preferably, the temperature of the bud catalyzing period is controlled to be 14-16 ℃, the humidity is controlled to be 98%, and the concentration of carbon dioxide is controlled to be 1000-2000mg/L-1The following.
Preferably, the cottonseed hull material has a higher proportion of constituents than sorghum.
The beneficial effects are as follows:
the industrial bottle cultivation of the hypsizygus marmoreus adopts advanced industrialized equipment and technology, realizes the automatic, standardized and large-scale production of the hypsizygus marmoreus, and the compost can be obtained locally, thereby greatly saving the transportation cost and the purchase cost, reducing the production cost of the hypsizygus marmoreus and improving the comprehensive economic value of the industrialized production of the hypsizygus marmoreus.
Detailed Description
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: an industrial bottle cultivation method for seafood mushrooms comprises the following operation steps:
preparation of materials
Preparing a mother culture medium and a stock culture medium, wherein the mother culture medium is a PDA culture medium; the stock culture medium is dry sawdust, rice bran and wheat bran; the composition ratio of the dry wood chips, the rice bran and the bran in the stock culture medium is 54%: 33.4%: 12.6 percent;
secondly, preparation of culture material
Preparing a stock culture medium and a mother culture medium, controlling the water content in the stock culture medium to be 60-65% and the pH value to be 7.2-7.5, mixing dry sawdust, rice bran and wheat bran in the mother culture medium with sorghum and cottonseed hull materials in proportion with the stock culture medium, standing, putting into a stirrer for stirring after fully absorbing water, and obtaining a culture material after stirring; the component proportion of the cottonseed hull material is higher than that of the sorghum;
thirdly, planting in a bottle
Sterilizing the culture materials, cooling, feeding the corresponding culture materials into an aseptic machine room for aseptic operation, bottling the culture materials by automatic mechanical equipment, and filling the corresponding stirred materials into a culture bottle; the selection of the culture bottle can be between 500 and 1000 ml;
cultivation of sea fresh mushroom
Firstly, the bottled culture material is fed into an automatic inoculation machine for strain inoculation treatment, after inoculation is completed, the culture material is fed into a culture chamber by a conveying mechanism,controlling proper temperature and carrying out reasonable culture; culturing for a certain number of days under the conditions of proper temperature, humidity and carbon dioxide concentration, and continuing to perform after-ripening culture for a certain number of days after the mycelia reach the bottom of the bottle, wherein the mycelia are sparse and turn into thick white to form thick and strong mycelia, the material surface is white and granular, and light yellow pigment is secreted, so that physiological maturity is achieved; the temperature of cultivation is controlled between 20-22 deg.C, the humidity is controlled at 65%, and the carbon dioxide concentration is controlled at 3000-4000mg/L-1The early stage culture days are about 50-55 days, and the later stage physiological maturity culture days are 30-35 days;
fifth, fruiting and harvesting of Hypsizygus marmoreus
After the strain grows over the bottle, entering a fruiting management stage, performing bottle opening and mycelium stimulation measures for conventional operation, injecting 10-15ml of water into the bottle through an industrial water injection mechanism, paving non-woven fabrics at the bottle mouth of the culture bottle, and controlling the culture temperature and humidity; when mushroom buds appear, removing the covered compound bars, controlling the culture temperature, spraying atomized water into the culture chamber by a water spraying mechanism, keeping the indoor humidity, carrying out normal ventilation treatment, controlling the carbon dioxide concentration, controlling the temperature at 14-16 ℃ during bud forcing period, controlling the humidity at 98%, and controlling the carbon dioxide concentration at 1000-2000mg/L-1The following; and waiting for a certain number of days, and picking the hypsizygus marmoreus by using picking equipment.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A seafood mushroom factory bottle cultivation method is characterized in that: the industrial cultivation method of the hypsizygus marmoreus comprises the following operation steps:
preparation of materials
Preparing a mother culture medium and a stock culture medium, wherein the mother culture medium is a PDA culture medium; the stock culture medium is dry sawdust, rice bran and wheat bran;
secondly, preparation of culture material
Preparing a stock culture medium and a mother culture medium, controlling the water content in the stock culture medium to be 60-65% and the pH value to be 7.2-7.5, mixing dry sawdust, rice bran and wheat bran in the mother culture medium with sorghum and cottonseed hull materials in proportion with the stock culture medium, standing, putting into a stirrer for stirring after fully absorbing water, and obtaining a culture material after stirring;
thirdly, planting in a bottle
Sterilizing the culture materials, cooling, feeding the corresponding culture materials into an aseptic machine room for aseptic operation, bottling the culture materials by automatic mechanical equipment, and filling the corresponding stirred materials into a culture bottle;
cultivation of sea fresh mushroom
Firstly, feeding bottled culture materials into an automatic inoculation machine for strain inoculation treatment, feeding the culture materials into a culture chamber by a conveying mechanism after inoculation is finished, and reasonably culturing by controlling a proper temperature; culturing for a certain number of days under the conditions of proper temperature, humidity and carbon dioxide concentration, and continuing to perform after-ripening culture for a certain number of days after the mycelia reach the bottom of the bottle, wherein the mycelia are sparse and turn into thick white to form thick and strong mycelia, the material surface is white and granular, and light yellow pigment is secreted, so that physiological maturity is achieved;
fifth, fruiting and harvesting of Hypsizygus marmoreus
After the strain grows over the bottle, entering a fruiting management stage, performing bottle opening and mycelium stimulation measures for conventional operation, injecting 10-15ml of water into the bottle through an industrial water injection mechanism, paving non-woven fabrics at the bottle mouth of the culture bottle, and controlling the culture temperature and humidity; and when mushroom buds appear, removing the covered compound bars, controlling the culture temperature, spraying atomized water into the culture room by a water spraying mechanism, keeping the indoor humidity, carrying out normal ventilation treatment, controlling the concentration of carbon dioxide, waiting for a certain number of days, and picking the hypsizygus marmoreus by picking equipment.
2. The industrialized bottle cultivation method for seafood mushrooms according to claim 1, which is characterized by comprising the following steps: the component ratio of the dry wood chips, the rice bran and the bran in the stock culture medium is 54%: 33.4%: 12.6 percent.
3. The industrialized bottle cultivation method for seafood mushrooms according to claim 1, which is characterized by comprising the following steps: the selection of the culture flask can be between 500 and 1000 ml.
4. The industrialized bottle cultivation method for seafood mushrooms according to claim 1, which is characterized by comprising the following steps: the temperature of the cultivation culture is controlled between 20-22 ℃, the humidity is controlled at 65%, and the carbon dioxide concentration is controlled at 3000-4000mg/L-1The number of days of early culture is about 50-55 days, and the number of days of late physiological maturation culture is 30-35 days.
5. The industrialized bottle cultivation method for seafood mushrooms according to claim 1, which is characterized by comprising the following steps: the temperature in the bud catalyzing period is controlled to be 14-16 ℃, the humidity is controlled to be 98%, and the concentration of carbon dioxide is controlled to be 1000-2000mg/L-1The following.
6. The industrialized bottle cultivation method for seafood mushrooms according to claim 1, which is characterized by comprising the following steps: the component proportion of the cottonseed hull material is higher than that of sorghum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110033407.4A CN112640728A (en) | 2021-01-11 | 2021-01-11 | Industrialized bottle cultivation method for seafood mushrooms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110033407.4A CN112640728A (en) | 2021-01-11 | 2021-01-11 | Industrialized bottle cultivation method for seafood mushrooms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112640728A true CN112640728A (en) | 2021-04-13 |
Family
ID=75367892
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110033407.4A Pending CN112640728A (en) | 2021-01-11 | 2021-01-11 | Industrialized bottle cultivation method for seafood mushrooms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112640728A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN102273378A (en) * | 2011-06-24 | 2011-12-14 | 郑雪平 | Bottle cultivation method for Hypsizigus marmoreus |
| CN106069189A (en) * | 2016-06-21 | 2016-11-09 | 桐城市佳明农业发展有限公司 | A kind of industrial culture method of seafood mushroom |
-
2021
- 2021-01-11 CN CN202110033407.4A patent/CN112640728A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN102273378A (en) * | 2011-06-24 | 2011-12-14 | 郑雪平 | Bottle cultivation method for Hypsizigus marmoreus |
| CN106069189A (en) * | 2016-06-21 | 2016-11-09 | 桐城市佳明农业发展有限公司 | A kind of industrial culture method of seafood mushroom |
Non-Patent Citations (2)
| Title |
|---|
| 刘会君;金国庆;黄亮;班立桐;杨华;王旭峰;: "海鲜菇工厂化瓶栽配方的优化" * |
| 季家举;谢元;: "工厂化海鲜菇瓶栽技术研究" * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101779574B (en) | Method for culturing of edible fungi | |
| CN1297654C (en) | Microbial ensiling strain and composite fungus, method for producing silage | |
| CN103210788B (en) | Pleurotus eryngii industrial production method | |
| CN101690554B (en) | Method of producing animal feed from straw | |
| CN102498930A (en) | Method for cultivating straw mushrooms by utilizing pleurotus eryngii fungi residues | |
| CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
| CN101743844A (en) | Cultivation method of white beech mushroom | |
| CN103396226A (en) | Pleurotus eryngii culture medium | |
| CN105237248A (en) | Grifola frondosa production culture medium and application thereof | |
| CN100535104C (en) | Method for preparing feedstuff yeast from maize peel hydrolysis solution | |
| CN106718021A (en) | A kind of yield Volvaria volvacea cultivation method high | |
| CN100535103C (en) | Method for preparing feedstuff yeast from maize peel hydrolysis sugar solution after extracting xylose | |
| CN1111098A (en) | Preparing process and use of fermented feed | |
| CN105646045A (en) | Straw mushroom culture medium | |
| CN107867934A (en) | A kind of culture medium for being used for true pleurotus cornucopiae and white beech mushroom and its preparation method and application | |
| CN112514734A (en) | Indoor cultivation method for phellinus igniarius | |
| CN105831435A (en) | Fermented feed improving meat production performance of goat and a preparing method thereof | |
| CN112640728A (en) | Industrialized bottle cultivation method for seafood mushrooms | |
| CN105110985B (en) | A kind of crab flavour mushroom compost and preparation method | |
| CN103332997A (en) | Raw material preparation method for cultivating flammulina velutipes after flammulina velutipes mushroom residue in-situ fermentation | |
| CN113966695A (en) | Hypsizigus marmoreus culture medium and preparation method and application thereof | |
| CN108157060B (en) | Hericium erinaceus culture medium, preparation method thereof and cultivation method of hericium erinaceus | |
| CN112931047A (en) | Method for manufacturing mushroom sticks of bag-cultivated mushrooms | |
| CN111480511A (en) | Cultivation and production process of fresh reed fungi | |
| CN111727811A (en) | Semi-synthetic morchella culture medium and nutrition bag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210413 |
|
| RJ01 | Rejection of invention patent application after publication |