CN1111098A - Preparing process and use of fermented feed - Google Patents
Preparing process and use of fermented feed Download PDFInfo
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- CN1111098A CN1111098A CN94105998A CN94105998A CN1111098A CN 1111098 A CN1111098 A CN 1111098A CN 94105998 A CN94105998 A CN 94105998A CN 94105998 A CN94105998 A CN 94105998A CN 1111098 A CN1111098 A CN 1111098A
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Abstract
The present invention relates to microbial engineering. Crushed and alkili-treated straw is oxygenic fermented with depth soil as carrier and in the presence of straw decomposing bacterium, bran water, cellulase, catalytic inorganic-salt activator, etc. and waste animal and plant protein with or without mixed depth soil is anaerobic fermented with cellulase and photosynthesis bacterium. The above-said two fermented products are mixed and anaerobic fermented to produce fermented straw powder feed, which is then mixed with grain feed, nutrient additive to produce the said fermented feed for various land and water animals.
Description
Present technique relates to one by three different steps, by anaerobism or aerobic process, nutrient fodder, its manufacture craft and the manufacture method thereof produced with bacterium, enzyme glycolysis grass meal and various discarded protein substance.
It is 87103054.3 that the Chinese patent communique discloses a patent No., name is called the technology of " fermented straw feed and preparation technology thereof ", be to adopt in the straw powder, to add salt, quick lime, rice-straw ash, warm water, hydrochloric acid, ammonium hydrogencarbonate and some trace element and enzyme, under the condition of anaerobism, be transformed.This method adds warm water with enzyme mixes well, alkaline solutions such as enzyme and quick lime (pH value 12.5-9).And enzyme (during pH value 4.5-5) competence exertion the best use of in sour environment, this method alkalescence has suppressed the vigor of enzyme.The Chinese patent communique also discloses in addition technology " pig with careless bar biological full mixed fodder ", and number of patent application 92109133.8 is methods of 16 days of basic treatment anaerobism behind some inorganic salts of interpolation.As complete feed, need and nutrition concentrated feed, energy feed, combine a nutrient fodder that overlaps growth of animal different times different proportion.It has utilized the variation of extraneous natural temperature, makes great efforts to make the various microorganisms in the grass meal obtain growth and breeding with chemical method deexcitation wild strain.The prescription of its employed trace element is: (preparation of peace 4g amount) vanadic anhydride 0.01g, manganese dioxide 0.02g, cobalt chloride 0.01g, manganese sulfate 0.005g, magnesium sulfate 0.005g, zinc oxide 0.005g, calcium carbonate 2.5g, ferrous sulfate 0.025g, copper sulphate 0.015g, aluminum sulfate 0.005g; The prescription of its preparation agent is: (preparation of peace 500g amount) quick lime 250g, refined salt 150g, sodium bicarbonate 50g, potash (plant ash) 50g, white sugar 5g, urea 0.25g.Fig. 1 is " pig with careless bar biological full mixed fodder " preparation schematic diagram, it is stirred in together by careless bar feed 100, nourishing additive agent 104 and energy feed 3 peace different proportions, wherein grass meal fodder 100 is after preparation agent 101, trace element 103 and careless bar powder 102 stir, and makes through 16 days anaerobic fermentations.Current society, utilize the saccharomycete enzyme to make fermented food, food, feed respectively, make contain higher protein, amino acid whose feed can be accomplished, but its cost height, fermentation time be long, make quality instability etc. (because a variety of causes, as careless bar because of producing region difference, climate temperature difference, water quality or the like).
The objective of the invention is, seek a new complete feed, be i.e. " three-step approach bacterium enzyme glycolysis feed ".It can bring into play the low characteristics of grass meal material cost, can bring into play the characteristics that high protein, amino acid feed are made in bacterium enzyme glycolysis fermentation again, produce a kind of Production Time short, cost is low, nutrition is high, the more desirable feed of making stable performance comes.
The present invention draws up a plan for according to following principle, the present invention has overcome the known technology anaerobic fermentation time long (reducing to three～eight days from 16 days), the alkalization back acidifying earlier of one of them major reason, alkalization make the coarse-fibred cell leakage of grass meal increase, and destroy its closely knit structure; Acidifying has replenished the composition of phosphorus and calcium, insert six strain quality yeast bacterium (every gram contains 11,600,000,000 living cells) and high activity again, have active photosynthetic bacteria and the micro-catalysis of inorganic salts of enriching B family vitamin high protein (every gram contains 65% albumen) and pay agent alive through screening, the more important thing is and introduced cellulase, straw decomposing and fermenting bacterium, adopt a high proportion of deep layer earth to make carrier.Meanwhile produce in addition and utilize industrial or agricultural waste residue albumen leftover bits and pieces, with six saccharomycetes fermentations twenty four hours, make it to be converted into homoamino acid active dry yeasr feed, with itself and grass meal yeast material co-fermentation, become bacterium enzyme glycolysis feed.As a whole, can save 50% grain feed as feed the present invention.Get a foothold (having grown the discarded compost behind the mushroom twice) as for the mushroom compost that adds 3%-5% in grass meal, because it has grown a large amount of trichodermins, and trichodermin is the thalline that produces cellulase.When fermenting together with other materials, the cellulase that trichodermin produced can decompose insoluble avicel cellulose, can decompose insoluble avicel cellulose, its mutant can also produce than wild bacterium and many 1-3 cellulase doubly; Cellulase produces a large amount of spore endoenzymes and spore exoenzyme; owing to make carrier with earth; soil is loose structure, does not live for a long time but protective enzyme is not subjected to ectocine, and the trace element in the earth is supplied with cellulose and produced enzyme; because fermentation temperature can be elevated to 35 ℃-55 ℃; can make enzyme that active performance is arranged, enzyme is had the leading role and the hydrolysis of degraded, act on cellulosic molecule than long-chain; primary product is a cellodextrin, cellobiose and cellotriose, the molten glucose that is situated between into of crude fibre.The cellulosan that the enzyme hydrolysis of B-glucoside is short generates glucose.A large amount of cellulases decompose by substrate, produce sugar in the sixth of the twelve Earthly Branches, pentose, pectin.Also have certain nutrient value and fruit fragrance and vinosity are arranged with the carbohydrate, alcohols, organic acid, the volatile fatty acid that decompose acquisition by fermentation, palatability is better.Open fermentation mainly is to allow the aerobic mushroom produce vigor, carries out glycolysis.In the amino acid whose while of synthetic proteins, carry out the 3rd step anaerobic fermentation again, mainly be saccharomycete part self-dissolving under anaerobic, improved protein and amino acid whose digesting and assimilating, Pasteur effect has increased.Can make spoilage organisms, mould anoxia asphyxia and dead under hypoxia condition, the content of organic acid, alcohols, volatile fatty acid etc. improves in the fermentate, and possesses vinosity, tart flavour, palatability.Anaerobic fermentation is not allow nitrogen volatilization and play fresh-keeping storage effect.In sour environment, through modulator, catalyst, pay the mutual alternating activity of various bacterium enzymes that agent alive makes adding and utilization and coexist, considerably beyond the vague generalization chemical catalyst, cellulolytic speed is higher 2.5 10 12 times than hydrionic catalytic efficiency through the catalytic capability that zymohydrolysis and cellulase produced that microbial fermentation produced.Come out through a large amount of development tests after the present invention program proposes, says on the whole, be with crude fibre, deep soil, hang down the albumen leftover bits and pieces and transform by high activity decomposing force strong zymophyte and enzyme.Promptly earlier by chemical basification, even because 70% sulfuric acid and concentrated hydrochloric acid all can not make cellulose dissolution, but aqueous slkali can make its decomposition and broken analysing; With conditioning agent it is adjusted to acidity then, make cellulase movable in the environment of acid and heat, because enzyme is to the inducing action of substrate and special selectivity, straw powder, straw powder, wood chip etc. all are the best needed substrates of cellulose, sugared source, N source (nitrogenous source), inorganic salts have been introduced in addition, some urge the enzyme agent also is to impel cellulase to produce spore endoenzyme and spore exoenzyme, and utilizing earth to make carrier, that cellulose is played mutual conduction lift-rising is ardent, and microbial reproduction speed is speeded.By earlier open aerobic fermentation, make enzyme at ventilated environment bottom fermentation, play the variation of matter, become single cell protein.With the common anaerobic fermentation of grass meal, strengthen the decomposing force of enzyme again.The complementary nutrition of the nutrition of yeast feed and grass meal fermentate need make amino acid, single cell protein content balance, can improve the digestibility of raw material, is rich in vitamin, necessary trace element and more active digestive ferment.
" three-step approach bacterium enzyme glycolysis feed manufacture craft and making apparatus " be three different steps, by anaerobism or aerobic process, nutrient fodder, its manufacture craft and the manufacturing equipment thereof produced with bacterium, enzyme glycolysis grass meal and various discarded protein substance.It is made through following process and step.(see figure 3) bacterium enzyme glycolysis forage nutrient fodder 1 is to stir the complete feed of making by different proportion by bacterium enzyme glycolysis grass meal fodder 2, energy feed 3, nutritious extract additive 4.The invention discloses the compound method of a whole set of feed making, they are compound methods of bacterium enzyme glycolysis feed 1, it is by bacterium enzyme glycolysis grass meal fodder 2, energy feed 3(grain feed) and nutritious extract additive 4 mix the complete feed of forming, the feature of its bacterium enzyme glycolysis grass meal fodder 2 compound methods is, it is mixed by bacterium enzyme glycolysis grass meal material base 25 and protein yeast feed 26 after anaerobic fermentation and making; Its bacterium enzyme glycolysis grass meal material base 25 alkali soak grass meal material 5 and are modulated into acidity after the glycolysis open fermentation of bacterium, enzyme is made through pH value conditioning agent 12; Its protein yeast feed 26 albumen leftover bits and pieces batching 6 open fermentations through the bacterium enzyme generate.The manufacture craft of a whole set of feed is also disclosed simultaneously, they are manufacture crafts of bacterium enzyme glycolysis feed, mix the feed of forming by bacterium enzyme glycolysis grass meal fodder 2, energy feed 3, nutritious extract additive 4, the feature of its manufacture craft is, the manufacture craft of bacterium enzyme glycolysis grass meal fodder 2, be the straw grass meal through alkaline leaching infiltration grass meal technology, acidification technique, bacterium enzyme glycolysis grass meal technology and through the albumen discarded object of albumen discarded object zymotechnique with after bacterium enzyme glycolysis grass meal mixes, again through bacterium enzyme glycolysis grass meal and protein feed co-fermentation technology composition; The manufacture craft of nutritious extract additive is that protein feed by the albumen discarded object zymotechnique production of 40% C scheme adds required batching again and stirs and make.So-called three-step approach, two processes are meant: in the manufacturing process of bacterium enzyme glycolysis grass meal fodder 2, through two processes of anaerobic and aerobic, divide three (see figure 4)s that step is carried out.The first step is that grass meal alkali soaks, make alkali and soak grass meal material 5, it is that bar by the straw of the various crops of careless bar powder 102(of being processed into powder such as straw, corn, beans, melon vine, Chinese sorghum etc. is processed into powder) add water, add infiltration preparation agent 15, (can be better) made through the open infiltration of twenty four hours if add 5% alfalfa meal and mushroom bacteria chaff effect again.Its manufacturing process is:
1). the plant straw is processed into the chaff shape, cross the sieve aperture that is less than or equal to 2mm, add enough water and make straw powder 102, as add mushroom after the pulverizing of grass meal weight 10% and cultivate leftover bits and pieces 23(mushroom and cultivate, gather the remaining culture in back mushroom and be called mushroom and cultivate leftover bits and pieces) and alfalfa meal 17 effects of grass meal weight 10% can be better.
2). preparation alkalescence infiltration preparation agent 15: with quick lime 31, salt 21, plant ash 33, sodium bicarbonate 34, sodium humate 13, to account for 0.5%, 0.4%, 0.1%, 0.1%, 0.25% preparation of grass meal weight separately, the water that adds 2.5 times is made.The pH value of this preparation agent is 12～13.
3). the preparation agent 15 that will infiltrate is poured in the straw powder 102 and is stirred, infiltration preparation agent 15 fully is penetrated in the straw powder 102, infiltration preparation agent 15 will soften or remove the closely macromolecule key of gathering of cellulose, hemicellulose, make alkali and soak grass meal material 5, and its pH value is 9.Infiltration is carried out under open good oxygen condition, needs 24 hours.Finished the first step this moment.
Second step had two ingredients to make simultaneously, and one is preparation bacterium enzyme glycolysis grass meal material base 25.Need open (aerobic) fermentation 24～40 hours after preparing, its fermentation temperature need reach more than 40 ℃ (must not above 55 ℃).Open aerobic fermentation is a process that heats up automatically, and the temperature of controlling well is necessary condition.Another is a preparation protein yeast feed 26, needs open fermentation 24 hours after preparing.The preparation method of bacterium enzyme glycolysis grass meal material base 25 has two schemes of A, B, and the A scheme is: (see figure 5) adds pH value conditioning agent 12, catalyst 11, cellulase 8, straw decomposer 9(in grass meal material 5 be the EM-1 bacterium), six saccharomycetes 10, liquid photosynthetic bacteria 16, zeolite powder 18(liquid photosynthetic bacteria 16 are adsorbed onto in the zeolite powder), the use of sieving of hydroxymethyl urea 14, straw decomposer 9, the deep soil of deep soil 27(below one meter), bran water 28, brown sugar 29 and suitable water is formulated.Stir after open fermentation in 24 to 40 hours and making, fermentation temperature needs mace more than 40 ℃ (must not above 55 ℃).The process for preparation and the configuration proportion of its A scheme are as follows:
1). the grass meal acidification: the glycolysis of bacterium enzyme must be carried out under acid state, and alkalescence not only can reduce zymohydrolysis, and might kill bacterium and enzyme.Its acidification process is to soak at alkali to add pH value conditioning agent 12 in the grass meal material 5, and pH value conditioning agent 12 is made of by 0.4% and 0.1% of grass meal weight separately calcium superphosphate 78 and ammonium sulfate 77, and adds an amount of hydrochloric acid 62.Ammonium sulfate 77 and hydrochloric acid 62 needs the water dilution with 0.5 times of grass meal weight, and the consumption of hydrochloric acid 62 depends on that conditioning agent 12 is admixed alkali soaks grass meal material 25, and its pH value reaches 1 for well.A large amount of conditioning agents makes the alkali of alkalescence soak grass meal material 25 and is adjusted to acid state rapidly.
2). preparation catalyst 11: catalyst 11 is the hydrogen ion inorganic salt microelement, and consumption is 0.042% of a grass meal weight.Its prescription is: vanadic anhydride 35, (its consumption is 0.03g) (all representing consumption in the parantheses), manganese dioxide 36(0.04g), cobalt chloride 37(0.03g), manganese sulfate 38(0.05g), magnesium sulfate 39(0.05g), inferior western sour sodium 40(0.05g), KI 41(0.1g), zinc oxide 42(1g), calcium carbonate 43(3.8g), ferrous sulfate 44(1.5g), copper sulphate 45(0.3g), aluminum sulfate 46(0.05g).
3). preparation bran water: in wheat bran 28, add entry and brown sugar 29, wheat bran 28 by grass meal weight 4%, brown sugar 29 by wheat bran weight 0.48%, water presses five times of preparations of wheat bran weight, ingredients needs to boil 15 minutes under 100 ℃ of temperature, perhaps opens bubbly water 5-10 minute at 100 ℃.Treat to put into when temperature is reduced to 40 ℃ cellulase 8 and catalyst 11, its consumption is respectively 0.8～1% and 0.042% of grass meal weight.
4). bubble is planted: the bacterium song (six saccharomycetes and straw decomposer) of dry powder shape is needed to pulverize in advance, soak, make bacterium resurrection, activation.One jin of dried bacterium adds five jin of water.Running water gets final product, and soaking needs twenty four hours (spring and autumn, the sky was three hours, two hours summers, four hours winters), and soaking needs to stir, and optimum temperature is 16 ℃～30 ℃.
5). 30: six saccharomycete 10 consumptions of preparation bacterium enzyme glycolysis liquid are 0.1%～0.2% of grass meal weight; Photosynthetic bacteria 16, consumption are 0.066% of grass meal weight; Zeolite powder 18, consumption are 0.416% of grass meal weight; Hydroxymethyl urea 14, consumption are 3.33% of grass meal weight; Straw decomposer 9(is the EM-1 bacterium), consumption is 0.5% of a grass meal weight.Straw decomposer 9, six saccharomycetes 10, all need the water of 0.4 times of weight own as inoculation water.
6). the preparation deep soil: (consumption account for complete feed 5%) takes from next meter, ground earth.Sieve, remove stone, sand.Deep soil need not contain assorted bacterium and microorganism, and perhaps the content of microorganism must not surpass the standard of feed defined.As admix some useless sugar, honey, sweet potato, potato, the meeting of fruit syrup effect better.
7). system bacterium enzyme glycolysis grass meal material base 25: with above said process 2), 3), 5), 6) and the mould activity made of the short and small mould fermented and cultured of cellulase 8(be the 286U/g international unit, consumption be grass meal weight 0.8%～1%) admix 1) in, because above-mentioned 1), 2), 3), 5), 6) to get the component of admixing be foundation with grass meal weight all in institute, therefore need only grass meal weight and determined that its component of admixing separately just has been determined.Stir open fermentation afterwards 20 hours to 40 hours.Humidity need satisfy 50%～95%, and this moment, pH value was 6～8, and fermentation time t and temperature c need satisfy relational expression t=760/c.
The B scheme is: (see figure 6)
1). the grass meal acidification: the glycolysis of bacterium enzyme must be carried out under acid state, its acidification process is to soak in the grass meal material 5 at alkali to add pH value conditioning agent 12, pH value conditioning agent 12 by calcium superphosphate 78 and ammonium sulfate 77 separately by grass meal weight 0.9% and 0.1% and an amount of hydrochloric acid 62 form.Ammonium sulfate 77 and hydrochloric acid 62 needs the water dilution with 0.5 times of grass meal weight, and the consumption of hydrochloric acid 62 depends on that conditioning agent 12 is admixed alkali soaks grass meal material 25, and its pH value reaches 1 for well.A large amount of conditioning agents makes the alkali of alkalescence soak grass meal material 25 and is adjusted to acid state rapidly.
2). preparation catalyst 11: catalyst 11 is the hydrogen ion inorganic salt microelement, and consumption is 0.042% of a grass meal weight.Its prescription is: vanadic anhydride 35, (its consumption is 0.03g) (all representing consumption in the parantheses), manganese dioxide 36(0.04g), cobalt chloride 37(0.03g), manganese sulfate 38(0.05g), magnesium sulfate 39(0.05g), inferior western sour sodium 40(0.05g), KI 41(0.1g), zinc oxide 42(1g), calcium carbonate 43(3.8g), ferrous sulfate 44(1.5g), copper sulphate 45(0.3g), aluminum sulfate 46(0.05g).
3). preparation bran water: in wheat bran 28, add entry and brown sugar 29, wheat bran 28 by grass meal weight 4%, brown sugar 29 by wheat bran weight 0.48%, water presses five times of preparations of wheat bran weight, ingredients needs to boil 15 minutes under 100 ℃ of temperature, perhaps opens bubbly water 5～10 minutes at 100 ℃.When treating that temperature is reduced to 40 ℃ cellulase 8 and catalyst 11 are added.
4). bubble is planted: the bacterium song (six saccharomycetes and straw decomposer) of dry powder shape is pulverized in advance, soaked, make bacterium bring back to life, activate.One jin of dried bacterium adds five jin of water.Running water gets final product, and soaking needs twenty four hours (spring and autumn, the sky was three hours, two hours summers, four hours winters), and soaking needs to stir, and optimum temperature is 16 ℃～30 ℃.
5). 30: six saccharomycete 10 consumptions of preparation bacterium enzyme glycolysis liquid are 0.1%-0.2% of grass meal weight; Photosynthetic bacteria 16, consumption are 0.005% of grass meal weight; Zeolite powder 18, consumption are 0.416% of grass meal weight; Straw decomposer 9(is the EM-1 bacterium), consumption is 0.5% of a grass meal weight.Straw decomposer 9, six saccharomycetes 10 all need the water of 0.4 times of weight own as inoculation water.
6). system bacterium enzyme glycolysis grass meal material base 25: with above said process 2), 3), 5) and the mould activity made of the short and small mould fermented and cultured of cellulase 8(be the 286U/g international unit, consumption be grass meal weight 0.8%～1%) admix 1) in, because above-mentioned 1), 2), 3), 5), 6) to get the component of admixing be foundation with grass meal weight all in institute, therefore need only grass meal weight and determined that its component of admixing separately just has been determined.Open fermentation is 20 hours to 40 hours after stirring.Humidity need satisfy 50%～95%, and this moment, pH value was 6～8, and fermentation time t and temperature c need satisfy relational expression t=760/c.
Narrating another ingredients that will prepare now is protein yeast feed 26, and preparation protein yeast feed 26 has two schemes of C, D.Narrate the process and the method (see figure 7) of C scheme (8Kg) below.The C scheme preparation method of protein yeast feed 26 is: add an amount of water in the albumen leftover bits and pieces 6 that processes, (1: 0.4 to 1: 0.45 material-water ratio) adds six saccharomycetes 10, and its ratio is 1%～1.2% of an albumen leftover bits and pieces.Photosynthetic bacteria 16, its ratio are 0.025% of albumen leftover bits and pieces; Add that salt 21 accounts for 5%, urea 22 accounts for 1%, ammonium sulfate 77 account for 2% and bone meal 19 account for 5%～8%.Carry out opening (aerobic) fermentation of twenty four hours after stirring, the product after the fermentation is exactly the protein yeast feed 26 that the C scheme is made.D scheme (23.6Kg) preparation method of protein yeast feed 26 is: add deep soil 27(13.6Kg the albumen leftover bits and pieces 6(8Kg that is processing)) (mixing blackstrap, sweet potato, potato, the meeting of fruit syrup effect better); Add six an amount of saccharomycetes 10, press 1%～1.2% about 240g～260g of D scheme gross weight; Photosynthetic bacteria 16 is pressed 0.025%～0.05% of D scheme gross weight, about 40g～60g; Add hydroxymethyl urea 14(2Kg), straw decomposer 9 is pressed 0.5% of D scheme total amount, about 118g; Add that salt 21 accounts for 5%, urea 22 accounts for 1%, ammonium sulfate 77 account for 2% and bone meal 19 account for 5%～8%, add water (the Total Water material-water ratio is 1: 0.4～1: 0.3).Carry out opening (aerobic) fermentation of twenty four hours after stirring, the product after the fermentation is exactly the protein yeast feed 26 that the D scheme is made.(see figure 8).Here be noted that, in the manufacturing process of preparation bacterium enzyme glycolysis grass meal fodder 2, the A of bacterium enzyme glycolysis grass meal material base 25 and the C and the D Scheme Selection of B scheme and protein yeast feed 26 can be the combinations respectively of A and C, D, also can be the combinations respectively of B and C, D.
The composition of the preparation of albumen leftover bits and pieces 6 is: account for 90% industrial or agricultural albumen discarded object 20, account for 4% salt 21, account for 1% ammonium sulfate 31, account for 1% urea 22 and account for 4% bone meal 19.Wherein industrial or agricultural albumen discarded object (as leftover bits and pieces of pluck etc.) need carry out can using after the high-temperature disinfection treatment.So-called industrial or agricultural albumen discarded object is meant, in industrial and agricultural production, as the discarded object of raw material that contains higher protein nutrition or product.Have such as vegetable raw material albumen discarded object 76: soybean cake 47, corn protein powder 48, cottonseed cake 49, rapeseed cake 50, oil cake of flax seed 51, bean vermicelli tail powder 52, vinasse 53, marc 54, sugarcane bagasse 55 and feed 56, castoff 57 etc., be not infected with the unmetamorphosed discarded object of germ.Animal raw materials albumen discarded object 75 has: discarded animal blood 58, animal viscera 59, silkworm chrysalis 60, aquatic livestock discarded object 70(small fish, shrimp head, dried small shrimp ...), hoof-and-horn meal 71, discarded leather powder 72(do not contain mashed skin), chicken manure 73, cow dung 74, feather meal 79 etc.Albumen leftover bits and pieces 6 can be fermented into the high-quality yeast feed by the fermentation of six saccharomycetes 10.Straw decomposer 9 can promote coarse-fibred decomposition with cellulase 8, and straw decomposer 9 and catalyst 11 can activate wild strain directional propagation in the grass meal together, also strengthens the fermenting power of deep soil 27 simultaneously, the activity of activation and catalyse cellulose enzyme.The proportioning of albumen leftover bits and pieces is: animal protein leftover bits and pieces 75 should account for 15%～20% of albumen leftover bits and pieces, and vegetable protein leftover bits and pieces 76 accounts for 70%～75%, and other compositions account for 5%～10%, and in its overall composition, Protein content need reach more than 40%.Experiment showed, that the content through fermentation protein leftover protein matter can improve 8%～16% on original basis.A large amount of amino acid and a large amount of living cells have been produced in the fermentation.The sweat of protein yeast feed 26 all has certain requirement to ventilation, humidity, temperature, can use booth ground fermentation method, fermentation vat thick-layer fermentation method, the block fermentation method of honeycomb, movable frame fermentation.The optimum temperature temperature of fermenting is 16 ℃～35 ℃, and optimum fermentation temperature is 30 ℃～38 ℃, the highlyest must not surpass 40 ℃, and the too high meeting of temperature makes aqtocytolysis.The time of fermentation generally was controlled at 20～30 hours, and fermentation time is relevant with fermentation temperature, and the high more time of temperature is just short more, otherwise then long more, the longest is 48 hours, if t represents the time, c represents mean temperature, and t and c satisfy relational expression t=760/c so.
Now several fermentation process with regard to protein yeast feed 26 are described in down:
1). booth ground fermentation: the ratio that control adds water be 1: 0.8～1(promptly 100 jin of siccatives add 80 jin of water, some say to be exactly that 100 jin albumen leftover bits and pieces adds 40 jin and bubble kind inoculation water material water and 40 jin in detail again).At the material 26 that upper berth, cement flooring 4-8 centimetre processes, (8 centimeters of winters, 4 centimeters of summers) Ventilation Control temperature.
2). the fermentation of fermentation vat thick-layer: fermentation vat is built on the ground and (is seen Fig. 9-1, Fig. 9-2 and Fig. 9-3), indoor fermentation vat 161 is made up of pond body 163, air blast 162, pond body 161 is made up of ventilating duct 166, ventilative seat 164, road base 167, discharge outlet 168, portable plate 169, expects 26 stacking the protein yeast fermentation that fermentate 165(promptly prepares on the ventilative seat on 164).Ventilating duct 166 by lower floor is by air blast oxygen supply and cooling, and fermentate is stacked thickness generally at 20～30 centimeters, every square meter average weight 30～50kg.Same design, thick-layer fermentation vat also can be built in underground, as long as sufficient move place is arranged on every side.
3). the block fermentation method of honeycomb: the protein yeast feed 26 for preparing is processed into honeycomb briquette formula protein yeast piece 192, piece 192 is put on the support body pallet 191, again pallet 91 is put on the carriage 190, can control ventilation and control temperature and humidity.The carriage of symmetry has location plush copper and recess, accurately location and installation mutually.The honeycomb yeast cutting is general stacks 5～10 layers, and every square metre of control weight is 20～30kg.
4). movable frame fermentation: on outdoor level land, can build up fixing and fermentation cabin (seeing Figure 10-1, Figure 10-2) movably, with movably fermenting vehicle (seeing Figure 11-1, Figure 11-2) operation on the storehouse, the protein yeast fermenting vehicle is made up of wheel 183, vehicle frame 180, dismountable pallet 181.Fermentation cabin can be barricaded as with plastics, and volume is decided according to the condition of output, control temperature, humidity, and its temperature can not surpass 40 ℃, and humidity is material-water ratio 1: 0.4.Finished product protein yeast feed 26 can directly be used for preparing full nutrition feed, also can drying, pulverizing, use as nutrient fodder.
The 3rd step was a preparation bacterium enzyme glycolysis grass meal fodder 2: the bacterium enzyme glycolysis grass meal material base 25 and the preparation protein yeast feed 26 that prepare are fitted over together, after stirring, at the condition bottom fermentation 3～8 days of sealing anaerobism, its finished product is exactly that bacterium enzyme glycolysis grass meal fodder 2(sees Fig. 4).
The preparation method of nutritious extract additive 4 is: the protein yeast feed 26 that accounts for the C scheme of whole ratio 40%, account for 5% bean cake powder 47, account for 3% corn steep liquor 48, account for 4% calcium monohydrogen phosphate 24, account for 2% photosynthetic bacteria 16, rare earth 63 accounts for 1%, methionine 64 accounts for 0.8%, lysine 65 accounts for 2%, cotton benevolence cake 49 accounts for 6%, gibberellin (920) 66 accounts for 0.025%, hydroxymethyl urea 14 accounts for 8%, sodium humate 13 accounts for 4%, citric acid 67 accounts for 2%, cellulase 8 accounts for 1%, bone meal 19 accounts for 6.75%, pre-ingredients 68 accounts for 4.4%, rape oil 69 accounts for 1%, salt 21 account for 4% and zeolite powder 18 account for 5%.About pre-ingredients 68, it is a kind of additional ingredients, be the additional ingredients of preparing according to the special circumstances of the local natural conditions of user, crop composition, environmental change, such as selecting a kind of like this prescription for use: (when gross weight is 88g) vitamin K1 .3g, riboflavin 1.6g, nicotinic acid 9g, B
126mg, folic acid 30mg, Cobastab
6250mg, biotin 18mg, manganese 4.5g, iron 15g, zinc 20g, copper 15g, iodine 45mg, selenium 25mg, vitamin A 2,000,000 units, vitamin D
3600,000 units, vitamin E 360 million international units etc.The manufactured goods of bacterium enzyme glycolysis grass meal fodder 2 have perfume (or spice), acid, soft, have the pungent flavor of wine alcohol, slightly being rich in protein, organic acid, enriching amino acid whose nutrient fodder like the apple flavor.Because the different growing stages of fowl poultry, be different to the needs of various nutritional labelings.It is by bacterium enzyme glycolysis grass meal fodder 2, energy feed 3, nutritious extract additive 4 that the present invention prepares a kind of complete feed (see figure 3) bacterium enzyme glycolysis feed 1 again, different growths, growth period according to different types of fowl poultry and fowl poultry, form in different ratio mixed preparing.
The present invention has following advantage:
1). with regard to quality, compare with domestic and international other fodder yeasts, mycoprotein has improved 33.85%, methionine, lysine and tryptophan have improved 127.69% ｀ 76.61% and 168.5% respectively, total amino acid content has improved 35.79%, high-quality thalline single cell protein, protein content be up to 50%-60%, can be all or the equivalent replacement imported fish meal, and be yeast feed with international most advanced level.It is mixed co-fermentation conversion in the grass powder biological feed, can make this protein hyperplasia to 18% of grass meal, data shows that straw contains crude protein 3.76%, NFE (soluble-carbohydrate) 41.9% crude fibre 32.6% ash 14.65%, straw does not contain amino acid and vitamin substantially.Two analysis reports are below arranged, the therefrom effect of bacterium enzyme glycolysis in improving grass meal nutrition as can be seen, table one is the nutritional labeling analysis report of straw powder before the glycolysis of bacterium enzyme;
| Sequence number | Project | Test result | Test basis |
| 1 | Moisture content % | 10.9 | GB6435 |
| 2 | Crude protein % | 8.6 | GB6432 |
| 3 | Coarse ash % | 8.2 | GB6438 |
| 4 | Calcium % | 1.56 | GB6436 |
| 5 | Phosphorus % | 0.89 | GB6437 |
| 6 | The yeast cells sum | 0 | Q/Q?JS02-90 |
| 7 | Total number of bacteria (hundred million/g) | 2.3 | Q/Q?JS02-90 |
| 8 | Crude fibre | 27.6 | GB6434 |
| 9 |
Table two is nutrition analysis reports of bacterium enzyme glycolysis grass meal.
| Sequence number | Test item | Test result | Test basis |
| 1 | Moisture content % | 11.2 | GB6435 |
| 2 | Crude protein % | 18.1 | GB6432 |
| 3 | Coarse ash % | 9.6 | GB6438 |
| 4 | Calcium % | 1.83 | GB6436 |
| 5 | Phosphorus % | 1.02 | GB6437 |
| 6 | The yeast cells sum | 8.3 hundred million/g | Q/Q?JS02-90 |
| 7 | Living cells ratio % | 93.6 | Q/Q?JS02-90 |
| 8 | Photosynthetic bacteria straw zymogenous bacteria sum (hundred million/g) | 126.3 | Q/Q?JS02-90 |
| 9 | Crude fibre | 18.7 | GB6434 |
| 10 | |||
| 11 |
2). make full use of a large amount of discarded objects in the society, just useless for precious, a large amount of grains have been saved, make that be used to the straw that makes a fire, abandoned industry, agricultural, sideline production waste residue leftover bits and pieces in a large number obtains comprehensive utilization, reasonably used microbial project, scientifically solved unemployed wide resource, for agricultural, animal husbandry provide new scientific and technological achievement.
3). with regard to the feed industry itself, bacterium enzyme glycolysis feed can be saved the grain of half in the raising of fowl poultry, make the cost of animal husbandry reduce more than 1/3rd, detect proof protein and reach about 18%, and contain active mycoprotein and 10,000,000,000 living cells, crude fibre only accounts for 19%.Need when feeding the fowl poultry and energy feed etc. fed together, and at this moment crude fibre on average has only below 8% in complete feed.
4). shortened the time of fodder production process greatly, comparable Technology Need 16 days, the present invention can shorten to four～Ninth Heaven.
The present invention has following accompanying drawing:
Fig. 1 is the grass meal complete feed preparation schematic diagram of known technology;
Fig. 2 is the grass meal complete feed process schematic representation of known technology;
Fig. 3 is a bacterium enzyme glycolysis feed preparation schematic diagram of the present invention;
Fig. 4 is a bacterium enzyme glycolysis grass meal fodder preparation schematic diagram of the present invention;
Fig. 5 is a bacterium enzyme glycolysis material base A scheme preparation schematic diagram of the present invention;
Fig. 6 is a bacterium enzyme glycolysis material base B scheme preparation schematic diagram of the present invention;
Fig. 7 is a protein yeast feed C scheme preparation schematic diagram of the present invention;
Fig. 8 is a protein yeast feed D scheme preparation schematic diagram of the present invention;
Fig. 9-the 1st, the fixed protein yeast air-flow of the present invention temperature adjustment fermentation vat schematic diagram;
Fig. 9-the 2nd, the fixed protein yeast air-flow of the present invention temperature adjustment fermentation vat schematic cross section;
Fig. 9-the 3rd, the fixed protein yeast air-flow of the present invention temperature adjustment fermentation vat vertical section schematic diagram;
Figure 10-the 1st, packaged type protein yeast fermentation cabin schematic diagram;
Figure 10-the 2nd, packaged type protein yeast fermentation cabin schematic side view;
Figure 11-the 1st, packaged type protein yeast fermenting vehicle schematic diagram;
Figure 11-the 2nd, packaged type protein yeast fermenting vehicle schematic side view;
Figure 12-the 1st, honeycomb fashion protein yeast fermentation frame schematic diagram;
Figure 12-the 2nd, honeycomb fashion protein yeast fermentation frame is shelved schematic diagram;
Figure 13 is a kind of prescription schematic diagram of albumen leftover bits and pieces 6 of the present invention;
Figure 14 is the schematic diagram of nutritious extract additive preparation composition of the present invention.
Now represent meaning to be described below respectively separately each label among the figure:
Fig. 1: nutritional agents 104, fowl poultry full price grass meal fodder 105;
Fig. 2: grass meal fodder 100, preparation agent add water 101, straw powder 102, trace element and add water 103;
Fig. 3: bacterium enzyme glycolysis feed 1, bacterium enzyme glycolysis grass meal fodder 2, energy feed 3, nutritious extract additive 4;
Fig. 4: alkali soaks grass meal material 5, infiltration preparation agent 15, alfalfa meal 17, mushroom cultivation leftover bits and pieces 23, bacterium enzyme glycolysis grass meal material base 25, protein yeast feed 26;
Fig. 5: cellulase 8, straw decomposer 9, six saccharomycetes 10, catalyst 11, pH value conditioning agent 12, hydroxymethyl urea 14, photosynthetic bacteria 16, zeolite powder 18, deep soil 27, bran water 28, brown sugar 29, bacterium enzyme glycolysis liquid 30;
Fig. 6: animal protein discarded object 75, vegetable protein discarded object 76;
Fig. 7: albumen leftover bits and pieces batching 6, sodium humate 13, bone meal 19, salt 21, urea 22;
Fig. 9-1, Fig. 9-2, Fig. 9-3: fermentation vat 161, air blast 162, pond body 163, ventilative seat 164, protein yeast fermentation material 165, ventilating duct 166, road base 167, discharge outlet 168, portable plate 169;
Figure 11: vehicle frame 180, pallet 181, wheel 183;
Figure 12-1, Figure 12-2: carriage (the symmetrical frame that riveting tenon mouth alive is arranged) 190, support body pallet 191, honeycomb briquette formula protein yeast piece 192;
Figure 13: cottonseed cake 49, rape cake 50, oil cake of flax seed 51, animal blood 58, aquatic livestock discarded object 70, discarded leather powder 72, beer extract 32, calcium monohydrogen phosphate 79;
Figure 14: dregs of beans cake 47, corn protein powder 48, rare earth 63, methionine 64, lysine 65, gibberellin 66, citric acid 67, calcium monohydrogen phosphate 24, sodium humate 13;
Narrating in figure, that narrates in specification also has: industrial or agricultural albumen discarded object 20, quick lime 31, plant ash 33, sodium bicarbonate 34, vanadic anhydride 35, manganese dioxide 36, cobalt chloride 37, manganese sulfate 38, magnesium sulfate 39, inferior western sour sodium 40, KI 41, zinc oxide 42, calcium carbonate 43, ferrous sulfate 44, copper sulphate 45, aluminum sulfate 46, bean vermicelli tail powder 52, alcohol 53, marc 54, bagasse 55, useless beverage 56, castoff 57, animal viscera 59, silkworm chrysalis 60, corn steep liquor 61, hydrochloric acid 62, pre-ingredients 68, rape oil 69, hoof-and-horn meal 71, chicken manure 73, cow dung 74, calcium superphosphate 78.
The present invention has following embodiment, all narrated in above specification about compound method of the present invention and process, the albumen that needs in an embodiment to replenish is got a foothold the preparation example of batching 6 and bacterium enzyme glycolysis feed 1 in concrete formulation examples in raising and feeding.As follows for get a foothold a kind of preparation composition and each component ratio of batching 6 of albumen: feather meal 79(2%), blood meal 58(2%), leather powder 72(10%), cotton benevolence cake 49(20%), rape cake 50(15%), oil cake of flax seed 51(15%), beer extract 32(10%), corn protein powder 48(10%), bone meal 19(4%), salt 21(4%), a fish shrimp head powder 70(3%).Material water was by preparation in 1: 4.Bacterium enzyme glycolysis feed is in the nursing of reality, different raising objects, all ages and classes, different budding raising object, the concrete use proportioning of its bacterium enzyme glycolysis grass meal fodder 2, energy feed 3, nutritious extract additive 4 is different, select different proportionings, then can raise the various animals of different aquatic or Lu Sheng respectively, wherein a kind of proportioning is undertaken by the method for table three.
Table three
| Project | Bacterium enzyme glycolysis grass meal battalion oxygen feed | Energy feed | The nutritious extract additive | Every day consumption |
| Below the small |
20%～35% | 55%～65% | 15%～20% | 0.5～1kg |
| 15kg～ |
30%～45% | 40%～50% | 10%～15% | 1～2kg |
| 30kg～60kg pig | 45%～60% | 40%～45% | 2%～5% | 2～3kg |
| 60kg～90kg pig | 60%～65% | 40%～45% | 2%～3% | 3～4kg |
| Conceived milking sow | 40%～45% | 45%～50% | 10%～15% | 3～4kg |
| Fryer, laying |
20%～30% | 55%～65% | 15% | |
| Milk cow | 35%～60% | 35%～60% | 5% | |
| Fish | 90%～100 | 5%～10% | 5% | |
| Duck, goose, rabbit, |
65%～70% | 15%～25% | 10% | |
Claims (23)
1, a kind of compound method of bacterium enzyme glycolysis feed, it is mixed the complete feed of forming by bacterium enzyme glycolysis grass meal fodder (2), energy feed (3) (grain feed) and nutritious extract additive (4), the feature of its bacterium enzyme glycolysis grass meal fodder (2) compound method is, it is mixed by bacterium enzyme glycolysis grass meal material base (25) and protein yeast feed (26) after anaerobic fermentation and making; Its bacterium enzyme glycolysis grass meal material base (25) alkali soaks grass meal material (5) and is modulated into acidity after the glycolysis open fermentation of bacterium, enzyme is made through pH value conditioning agent (12); Its protein yeast feed (26) albumen leftover bits and pieces batching (6) generates through the open fermentation of bacterium enzyme.
2, the compound method of feed according to claim 1, its alkali soaks the preparation composition of grass meal material (5) and the feature of each component ratio is, it is by the careless bar powder (102) that is processed into powder, it is that the bar of the straws of various crops such as straw, corn, beans, melon vine, Chinese sorghum etc. is processed into powder and makes, add five times water, make through infiltration preparation agent (15) infiltration, if add again the alfalfa meal (17) that accounts for grass meal weight 10%, 10% mushroom cultivate leftover bits and pieces (23) stir effect can be better.
3, the compound method of feed according to claim 1, the preparation composition of the bacterium enzyme glycolysis grass meal material base (25) of its A scheme and the feature of each component ratio are, alkali soaks and adds pH value conditioning agent (12) in the grass meal material (5), catalyst (11), wheat bran (28) is pressed 4% of grass meal weight, brown sugar (29) is pressed 0.48% of wheat bran weight, water is pressed five times of preparations of wheat bran weight, put into cellulase 8 and catalyst (11), its consumption is respectively 0.8～1% and 0.042% of grass meal weight, add the bacterium enzyme glycolysis liquid (30) of A scheme and deep soil (27) (consumption account for complete feed 5%) and take from next meter, ground earth, sieve, remove stone, sand is admixed some useless sugar, honey, sweet potato, potato, the fruit syrup effect can be better.
4, the compound method of feed according to claim 1, the preparation composition of the bacterium enzyme glycolysis grass meal material base (25) of its B scheme and the feature of each component ratio are, soak in the grass meal material (5) at alkali and to add pH value conditioning agent (12), pH value conditioning agent (12) by calcium superphosphate (78) and ammonium sulfate (77) separately by grass meal weight 0.9% and 0.1% and an amount of hydrochloric acid (62) form.Ammonium sulfate (77) and hydrochloric acid (62) need the water dilution with 0.5 times of grass meal weight, the consumption of hydrochloric acid (62) depend on pH value conditioning agent (12) admixed alkali and soaks grass meal material (25) after, its pH value reaches 1 for well; Catalyst (11), wheat bran (28) by grass meal weight 4%, brown sugar (29) by wheat bran weight 0.48%, water presses five times of preparations of wheat bran weight, put into cellulase (8) and catalyst (11), its consumption is respectively 0.8～1% and 0.042% of grass meal weight, adds the bacterium enzyme glycolysis liquid (30) of B scheme.
5, the compound method of feed according to claim 1, the preparation composition of the protein yeast feed (26) of its C scheme and the feature of each component ratio are, in the albumen leftover bits and pieces (6) that processes, add an amount of water, (1: 0.4 to 1: 0.45 material-water ratio) adds six saccharomycetes (10), and its ratio is 1%～1.2% of an albumen leftover bits and pieces.Photosynthetic bacteria (16), its ratio are 0.025% of albumen leftover bits and pieces; Add that salt (21) accounts for 5%, urea (22) accounts for 1%, ammonium sulfate (77) account for 2% and bone meal (19) account for 5%～8%.
6, the compound method of feed according to claim 1, the preparation composition of the protein yeast feed (26) of its D scheme and the feature of each component ratio be, adds deep soil (27) (13.6Kg) (mix blackstrap, sweet potato, potato, fruit syrup effect can better) in (8Kg) at the albumen leftover bits and pieces (6) that processes; Add an amount of six saccharomycetes (10), press 1%～1.2% about 240g～260g of D scheme gross weight; Photosynthetic bacteria (16) is pressed 0.025%～0.05% of D scheme gross weight, about 40g～60g; Add hydroxymethyl urea (14) (2Kg), straw decomposer (9) is pressed 0.5% of D scheme total amount, about 118g; Add that salt (21) accounts for 5%, urea (22) accounts for 1%, ammonium sulfate (77) account for 2% and bone meal (19) account for 5%～8%, add water (the Total Water material-water ratio is 1: 0.4～1: 0.3).
7, the compound method of feed according to claim 1, the preparation composition of its nutritious extract additive (4) and the feature of each component ratio are to account for the protein yeast feed (26) of the C scheme of whole ratio 40%, account for 5% bean cake powder (47), account for 3% corn steep liquor (48), account for 4% calcium monohydrogen phosphate (24), account for 2% photosynthetic bacteria (16), rare earth (63) accounts for 1%, methionine (64) accounts for 0.8%, lysine (65) accounts for 2%, cotton benevolence cake (49) accounts for 6%, gibberellin " 920 " (66) accounts for 0.025%, hydroxymethyl urea (14) accounts for 8%, sodium humate (13) accounts for 4%, citric acid (67) accounts for 2%, cellulase (8) accounts for 1%, bone meal (19) accounts for 6.75%, pre-ingredients (68) accounts for 4.4%, rape oil (69) accounts for 1%, salt (21) account for 4% and zeolite powder (18) account for 5%.
8, the compound method of feed according to claim 1, the feature of the configuration proportion of its bacterium enzyme glycolysis grass meal fodder (2), energy feed (3), nutritious extract additive (4) is that one of them uses proportioning to be undertaken by the method for following table three:
Table three
Project
Bacterium enzyme glycolysis grass meal battalion oxygen feed
Energy feed
The nutritious extract additive
Every day consumption
Below the small weaning pig 12kg
20%～35%
55%～65%
15%～20%
0.5～1kg
15kg～30kg pig
30%～45%
40%～50%
10%～15%
1～2kg
30kg～60kg pig
45%～60%
40%～45%
2%～5%
2～3kg
60kg～90kg pig
60%～65%
40%～45%
2%～3%
3～4kg
Conceived milking sow
40%～45%
45%～50%
10%～15%
3～4kg
Fryer, laying hen
20%～30%
55%～65%
15%
Milk cow
35%～60%
35%～60%
5%
Fish
90%～100
5%～10%
5%
Duck, goose, rabbit, bird
65%～70%
15%～25%
10%
9, the compound method of feed according to claim 2, the preparation composition of its infiltration preparation agent (15) and the feature of each component ratio are, with quick lime (31), salt (21), plant ash (33), sodium bicarbonate (34), sodium humate (13), to account for 0.5%, 0.4%, 0.1%, 0.1%, 0.25% preparation of grass meal weight separately, the water that adds 2.5 times is made.The pH value of this preparation agent is 12～13.
10, the compound method of feed according to claim 3, the preparation composition of the bacterium enzyme glycolysis liquid (30) of its A scheme and the feature of each component ratio are that six saccharomycetes (10) consumption is 0.1%～0.2% of a grass meal weight; Photosynthetic bacteria (16), consumption are 0.066% of grass meal weight; Zeolite powder (18), consumption are 0.416% of grass meal weight; Hydroxymethyl urea (14), consumption are 3.33% of grass meal weight; Straw decomposer (9) (being the EM-1 bacterium), consumption is 0.5% of a grass meal weight.Straw decomposer (9), six saccharomycetes (10), all need the water of 0.4 times of weight own as inoculation water.
11, the compound method of feed according to claim 4, the preparation composition of the bacterium enzyme glycolysis liquid (30) of its B scheme and the feature of each component ratio are that six saccharomycetes (10) consumption is 0.1%～0.2% of a grass meal weight; Photosynthetic bacteria (16), consumption are 0.005% of grass meal weight; Zeolite powder (18), consumption are 0.416% of grass meal weight; Straw decomposer (9) (being the EM-1 bacterium), consumption is 0.5% of a grass meal weight, straw decomposer (9), six saccharomycetes (10) all need the water of 0.4 times of weight own as inoculation water.
12, compound method according to claim 3 or 4 described feeds, the preparation composition of its catalyst (11) and the feature of each component ratio are, vanadic anhydride (35), (its consumption is 0.03g) (all representing consumption in the parantheses), manganese dioxide (36) (0.04g), cobalt chloride (37) (0.03g), manganese sulfate (38) (0.05g), magnesium sulfate (39) (0.05g), inferior western sour sodium (40) (0.05g), KI (41) (0.1g), zinc oxide (42) (1g), calcium carbonate (43) (3.8g), ferrous sulfate (44) (1.5g), copper sulphate (45) (0.3g), aluminum sulfate (46) (0.05g).
13, according to the compound method of claim 5 or 6 described feeds, the preparation composition of its albumen leftover bits and pieces batching (6) and the feature of each component ratio are, animal protein leftover bits and pieces (75) should account for 15%～20% of albumen leftover bits and pieces, vegetable protein leftover bits and pieces (76) accounts for 70%～75%, other compositions account for 5%～10%, in its overall composition, Protein content need reach more than 40%.
14, compound method according to claim 5 or 6 described feeds, a kind of feature of preparing composition and each component ratio of its albumen leftover bits and pieces batching (6) is, and is as follows for a kind of preparation composition and each component ratio of albumen leftover bits and pieces batching (6): feather meal (79) (accounting for 2%), blood meal (58) (accounting for 2%), leather powder (72) (accounting for 10%), cotton benevolence cake (49) (accounting for 20%), rape cake (50) (accounting for 15%), oil cake of flax seed (51) (accounting for 15%), beer extract (32) (accounting for 10%), corn protein powder (48) (accounting for 10%), bone meal (19) (accounting for 4%), salt (21) (accounting for 4%), a fish shrimp head powder (70) (accounting for 3%).Material water was by preparation in 1: 4.
15, the compound method of feed according to claim 13, the feature of the preparation composition of its albumen leftover bits and pieces batching (6) is, its vegetable raw material albumen discarded object (76) can be selected soybean cake (47), corn protein powder (48), cottonseed cake (49), rapeseed cake (50), oil cake of flax seed (51), bean vermicelli tail powder (52), vinasse (53), marc (54), sugarcane bagasse (55) and beverage (56), castoff (57) etc. for use, is not infected with the unmetamorphosed discarded object of germ.Animal raw materials albumen discarded object (75) can be selected discarded animal blood (58), animal viscera (59), silkworm chrysalis (60), aquatic livestock discarded object (70) (small fish, shrimp head, dried small shrimp for use ...), hoof-and-horn meal (71), discarded leather powder (72) (not containing mashed skin), chicken manure (73), cow dung (74), feather meal (79) etc.
16, the manufacture craft of bacterium enzyme glycolysis feed is mixed the feed of forming by bacterium enzyme glycolysis grass meal fodder (2), energy feed (3), nutritious extract additive (4), and the feature of its manufacture craft is,
1). the manufacture craft of bacterium enzyme glycolysis grass meal fodder (2), be straw grass meal raw material through raw material through alkaline leaching infiltration grass meal technology, second step, two technology acidification techniques, bacterium enzyme glycolysis grass meal technology and albumen discarded object zymotechniques carried out simultaneously, and the 3rd step is again through bacterium enzyme glycolysis grass meal and protein feed co-fermentation technology and select corresponding open aerobic or seal that two processes of anaerobism carry out;
2). the manufacture craft of nutritious extract additive is that protein feed by the albumen discarded object zymotechnique production of 40% C scheme adds required batching again and stirs and make.
17, manufacture craft according to claim 16, the feature of its alkaline process infiltration grass meal is, the preparation agent (15) that will infiltrate is poured in the straw powder (102) and is stirred, infiltration preparation agent (15) fully is penetrated in the straw powder (102), infiltration preparation agent (15) will soften or remove the closely macromolecule key of gathering of cellulose, hemicellulose, make alkali and soak grass meal material (5), its pH value is 9.Infiltration is carried out under open good oxygen condition, needs 24 hours.
18, manufacture craft according to claim 16, the feature of its acidification technique is, at pH value is that 9 alkali soaks and adds pH value conditioning agent (12) in the grass meal material (5), pH value conditioning agent (12) is made up of calcium superphosphate (78) and ammonium sulfate (77) and hydrochloric acid (62), the consumption of hydrochloric acid (62) depend on conditioning agent 12 admixed alkali and soaks grass meal material (25) after, make its pH value reach 1.
19, manufacture craft according to claim 16, the feature of its bacterium enzyme glycolysis technology are to stir open fermentation afterwards 20 hours to 40 hours after bacterium enzyme glycolysis grass meal material base 25 required raw materials are assorted.Humidity need satisfy 50%～95%, and this moment, pH value was 6～8, and fermentation time t and temperature c need satisfy relational expression t=760/c.
20, manufacture craft according to claim 16, the feature of its albumen discarded object zymotechnique is, after the required raw material of protein yeast feed (26) assorted, carry out opening (aerobic) fermentation of twenty four hours after stirring, the product after the fermentation is exactly the protein yeast feed (26) that the D scheme is made.The optimum temperature temperature of fermenting is 16 ℃～35 ℃, and optimum fermentation temperature is 30 ℃～38 ℃, the highlyest must not surpass 40 ℃, and the too high meeting of temperature makes aqtocytolysis.The time of fermentation generally was controlled at 20～30 hours, and fermentation time is relevant with fermentation temperature, and the high more time of temperature is just short more, otherwise then long more, the longest is 48 hours, if t represents the time, c represents mean temperature, and t and c satisfy relational expression t=760/c so.
21, manufacture craft according to claim 16, its bacterium enzyme glycolysis grass meal material base (25) with the feature of protein yeast feed (26) co-fermentation technology is, the bacterium enzyme glycolysis grass meal material base (25) and the preparation protein yeast feed (26) that prepare are fitted over together, after stirring, at the condition bottom fermentation 3～8 days of sealing anaerobism, its finished product is exactly a bacterium enzyme glycolysis grass meal fodder (2).
22, manufacture craft according to claim 16, because bacterium enzyme glycolysis grass meal material base (25) has two schemes of A, B, protein yeast feed (26) has two schemes of C, D, its bacterium enzyme glycolysis grass meal material base (25) with the technology characteristics of protein yeast feed (26) co-fermentation is, when the fermentation preparation can be the combination respectively of A and C, D, also can be the combination respectively of B and C, D.
23, the purposes of bacterium enzyme glycolysis feed, it is characterized in that at different raising objects, as long as choose bacterium enzyme glycolysis grass meal fodder (2), energy feed (3) and the different proportioning of nutritious extract additive (4), can raise the various animals of aquatic or Lu Sheng respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94105998A CN1111098A (en) | 1994-06-10 | 1994-06-10 | Preparing process and use of fermented feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94105998A CN1111098A (en) | 1994-06-10 | 1994-06-10 | Preparing process and use of fermented feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1111098A true CN1111098A (en) | 1995-11-08 |
Family
ID=5032323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94105998A Pending CN1111098A (en) | 1994-06-10 | 1994-06-10 | Preparing process and use of fermented feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1111098A (en) |
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|---|---|---|---|---|
| WO1999057991A1 (en) * | 1998-05-12 | 1999-11-18 | Jungong Xiong | A method for preparing leaf proteins with rumex l. and obtained product thereby |
| US6706256B2 (en) | 2001-05-15 | 2004-03-16 | The Procter & Gamble Co. | Oral care compositions |
| CN101798145A (en) * | 2010-03-02 | 2010-08-11 | 都江堰惠农生物技术有限责任公司 | Production method of microbial fish guano |
| CN103976179A (en) * | 2014-05-03 | 2014-08-13 | 内蒙古凯嘉生物技术有限责任公司 | Ecological fermented feed, and preparing method and application thereof |
| CN105211633A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Efficient straw biology feed additive manufactures and using method |
| CN105360611A (en) * | 2015-11-20 | 2016-03-02 | 六安市裕皖生物科技有限公司 | Pig plasma protein powder-added sheep feed and preparation method thereof |
| CN106107050A (en) * | 2016-06-26 | 2016-11-16 | 南宁学院 | A kind of method utilizing straw Fodder making |
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1994
- 1994-06-10 CN CN94105998A patent/CN1111098A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999057991A1 (en) * | 1998-05-12 | 1999-11-18 | Jungong Xiong | A method for preparing leaf proteins with rumex l. and obtained product thereby |
| US6706256B2 (en) | 2001-05-15 | 2004-03-16 | The Procter & Gamble Co. | Oral care compositions |
| CN101798145A (en) * | 2010-03-02 | 2010-08-11 | 都江堰惠农生物技术有限责任公司 | Production method of microbial fish guano |
| CN103976179A (en) * | 2014-05-03 | 2014-08-13 | 内蒙古凯嘉生物技术有限责任公司 | Ecological fermented feed, and preparing method and application thereof |
| CN105211633A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Efficient straw biology feed additive manufactures and using method |
| CN105360611A (en) * | 2015-11-20 | 2016-03-02 | 六安市裕皖生物科技有限公司 | Pig plasma protein powder-added sheep feed and preparation method thereof |
| CN106107050A (en) * | 2016-06-26 | 2016-11-16 | 南宁学院 | A kind of method utilizing straw Fodder making |
| CN106578702A (en) * | 2016-12-08 | 2017-04-26 | 永登富源农业科技发展有限公司 | Preparation method for fish feed |
| CN106578702B (en) * | 2016-12-08 | 2020-10-23 | 永登富源农业科技发展有限公司 | Preparation method of fish feed |
| CN108338125A (en) * | 2018-02-07 | 2018-07-31 | 张掖市金农源生物科技有限公司 | A kind of breeding pannage and its cultural method |
| CN109090144A (en) * | 2018-10-17 | 2018-12-28 | 马书文 | A kind of novel environmentally-friendly biological herbicide |
| CN109730195A (en) * | 2019-03-15 | 2019-05-10 | 刘坚 | A kind of animal sources pannage and preparation method thereof |
| CN113142399A (en) * | 2021-05-27 | 2021-07-23 | 山西省农业科学院饲料兽药研究所 | Technical process for preparing cattle and sheep fermented feed by using fungus sticks |
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