CN112626059A

CN112626059A - Net bottom cleaning bacterium for cleaning silt at net cage bottom and preparation method thereof

Info

Publication number: CN112626059A
Application number: CN202011203113.3A
Authority: CN
Inventors: 邹国忠
Original assignee: Wuxi Hongbao Microorganism Technology Co ltd
Current assignee: Wuxi Hongbao Microorganism Technology Co ltd
Priority date: 2020-11-02
Filing date: 2020-11-02
Publication date: 2021-04-09

Abstract

The invention discloses a preparation method of dictyostelium, which comprises 0.6-2.5% of liquid bacillus, 35-53% of bentonite, 35-53% of silicate, 2-6% of sodium percarbonate, 0.5-1.5% of protease and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate, so that the preparation method has the effect of degrading excrement, residual bait, animal and plant carcasses and organic debris at the bottom of a net cage.

Description

Net bottom cleaning bacterium for cleaning silt at net cage bottom and preparation method thereof

Technical Field

The invention belongs to the technical field of aquaculture water body purification and microorganism treatment, and particularly relates to net bottom cleaning bacteria for net cage bottom dredging and a preparation method thereof.

Background

The net cage fish culture is a production mode of placing a cage made of net sheets in a certain water area for fish culture. The net cages are mostly arranged in lakes, rivers, reservoirs and other water areas with certain water flow, fresh water quality and high dissolved oxygen. The high-density intensive culture can be realized, and the yield per square meter can reach dozens of kilograms to dozens of kilograms according to the bottom area of the net cage.

Because the shoal of fish is restricted in the box with a net, excrement and urine, incomplete bait, animal and plant corpses and organic debris that the shoal of fish produced can pile up at the box with a net bottom, form silt, finally lead to the box with a net bottom blacken and foul, and the current generally is through the manual cleaning box with a net, waste time and energy.

The invention aims to provide a net bottom bacterium cleaning method, which degrades excrement, residual bait, animal and plant corpses and organic debris at the bottom of a net cage through microorganisms and avoids the blackening and stinking of the bottom of the net cage.

Disclosure of Invention

In view of the disadvantages of the prior art, it is an object of the present invention to provide a net bottom cleaning bacterium having an effect of degrading feces, residual baits, animal and plant corpses and organic debris at the bottom of a net cage.

In order to achieve the technical purpose, the invention provides the following technical scheme: the dictyoshinium, which comprises 0.6-2.5% of liquid bacillus, 35-53% of bentonite, 35-53% of silicate, 2-6% of sodium percarbonate, 0.5-1.5% of proteolytic enzyme and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate.

Preferably, the liquid bacillus is liquid bacillus natto, and each ml of the liquid bacillus contains more than 100 hundred million live bacteria.

Preferably, the applicable temperature is 10-40 ℃, and the pH value of the applicable water body is 6.0-10.0.

Preferably, 40g of mesh bottom clear bacteria is used per square meter of water and thrown once every 7-10 days.

Aiming at the defects in the prior art, the invention also aims to provide a preparation method of the dictyostelium sp.

In order to achieve the technical purpose, the invention provides the following technical scheme: a preparation method of dictyostelium sp comprises the following steps:

s1, weighing granular silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading the granular silicate, uniformly spraying liquid bacillus with the formula amount on the silicate, uniformly mixing the raw materials, weighing the bentonite raw material with the formula amount, and mixing the bentonite raw material with the raw material mixture;

s2, weighing a sodium percarbonate raw material and a protease according to the formula amount, and uniformly stirring and mixing;

and S3, mixing the mixture obtained in the step S1 and the mixture obtained in the step S2 again to obtain a mixture, then putting the mixture into a stirrer, adding physiological saline with the formula amount, fully stirring and mixing to meet the granulating requirement, and finally putting the mixture into a granulator to prepare granules.

In summary, the present invention achieves the following effects:

1. and (3) increasing dissolved oxygen: the oxidation-reduction potential and dissolved oxygen at the bottom of the net cage are improved, the mass propagation of aerobic probiotics is promoted, and the growth of anaerobic harmful bacteria is inhibited;

2. improving the substrate: degrading residual bait, excrement, animal and plant corpses and organic debris at the bottom of the net cage;

3. purifying water quality: effectively adsorbing harmful substances such as ammonia nitrogen, metasilicate, hydrogen sulfide, algal toxin and the like;

4. stabilizing the pH value of the water body, supplementing trace elements, keeping the proportion balance of probiotics and plankton in the water, and inhibiting the propagation and growth of harmful algae;

5. the transparency of the water body is improved, and the normal operation of the photosynthesis of plankton in the water is ensured;

6. the product is a mechanical-compression granular preparation, has high survival rate of viable bacteria, is directly thrown, and is convenient to use;

7. the product has wide temperature range, and viable bacteria can grow and propagate at water temperature of 10-40 deg.C;

8. the product has strong adaptability and strong tolerance to chemical disinfection drugs, insecticidal drugs, antibiotics and the like;

9. the product is suitable for water body with pH of 6.0-10, and is suitable for various aquaculture water bodies.

Detailed Description

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

The first embodiment is as follows: a strain for cleaning the bottom of a net comprises 2.5% of liquid bacillus, 48% of bentonite, 52% of silicate, 6% of sodium percarbonate, 1.5% of proteolytic enzyme and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate. As the silicate, orthoclase (K [ AlSi3O8]), albite (Na [ AlSi3O8]), kaolinite (Al4[ Si4O10] [ OH ]8), anorthite (Ca [ Al2Si2O8]), talc (Mg3Si4O10[ OH ]2), muscovite (KAl2[ AlSi3O10] [ OH ]2), quartz (SiO2), opal (SiO 2. nH2O) and the like can be used

The liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus natto contains more than 100 hundred million live bacteria. The applicable temperature is 10-40 ℃, and the pH value of the applicable water body is 6.0-10.0.

The bacteria are cleared by using 40g of net bottom per square meter of water body and are thrown once every 7 to 10 days.

[ physicochemical Properties ] yellow-white granules.

[ Main Components ] Bacillus, protease, silicate, bentonite, sodium percarbonate, etc.

[ active ingredient content ] Probiotics 10 x 10^8cfu/g

[ APPLICATION RANGE ] is suitable for cage culture and cultivation of marine and freshwater aquatic animals in south and north, and is especially suitable for improving and repairing the net bottom micro-ecological environment of high-density cage culture.

[ DOSAGE ] can be directly and uniformly thrown by dry method.

[ dosage ] 40g of the product is directly thrown every square meter and every 7-10 days, and can be used twice when the bottom of the net cage is seriously deteriorated.

[ PACKAGE ] 5 kg/bag.

[ STORAGE ] is preserved in a cool, dry and ventilated place for 24 months.

A preparation method of dictyostelium sp comprises the following steps:

The application case of the net bottom cleaning bacteria in the net cage culture of the grass carps for cleaning the sludge at the bottom of the net cage is as follows:

treating a water body: the application test is carried out in the water area where fresh water net cages are used for culturing the grass carps in the mountainous lake region of Shandong Jining Luqiangzhen town, the water depth of the culturing water area is about 3 meters, the area of each net cage is about 30 square meters, the water depth in the net cages is 2 meters, the net cages are arranged in series, the distance of 5 meters is kept between the single net cages, the distance between the rows of the net cages arranged in series is kept about 30 meters, and the offshore distance of the side, close to the bank, of the net cages is 15 meters. And (2) breeding the fingerlings in 20 days after 2 months, wherein the specification of the fingerlings to be placed into the net cages is about 0.25kg per fish, grass carp is taken as a main part, a small amount of catfishes, chubs, carps and breams are properly matched, the fingerlings are strong in physique and consistent in specification, 800 grass carp seeds with the specifications are placed into each net cage, and 10-20 fish seeds with the same specification are matched with the chubs, the carps and the breams. Soaking the fingerlings in 3% saline solution for 5 minutes before putting the fingerlings into the water tank for fish body disinfection, collecting water samples in the net cage and the bottom environment of water outside the net cage during fish seed stocking for physicochemical index measurement, and obtaining data as follows: 0.2mg/L of ammonia nitrogen, 0.05mg/L of nitrite nitrogen, 0.02mg/L of hydrogen sulfide, 8.7 of pH value and 35 cm of sludge at the bottom outside the net cage. Cleaning sludge at the outer bottom of the net cage by applying net bottom cleaning bacteria, wherein the treatment period is 6 months, the sludge is used for 1 time every 10 days in the first 2 months, and 40g of net bottom cleaning bacteria are splashed at the bottom of the net cage per square meter; the product is used every 7 days for 4 months, and 40g of net bottom cleaning bacteria are sprinkled on the bottom of the net cage per square meter. After the treatment is finished, collecting a water sample in the net cage and the bottom environment of the water outside the net cage to perform physical and chemical index measurement, and obtaining data as follows: 0.1mg/L of ammonia nitrogen, 0.01mg/L of nitrite nitrogen, 0.01mg/L of hydrogen sulfide, 8.5 of pH value and 25 cm of sludge at the bottom outside the net cage.

The implementation case shows that the net bottom cleaning bacteria have obvious improvement effect on the water area environment of the net cage culture, and the net bottom cleaning bacteria are solid particles, have the advantages of rapid bottom settlement, effective permeation to the bottom of the net cage and strong slow release effect, so that sludge at the bottom outside the net cage is rapidly decomposed, converted and eliminated; meanwhile, an ammonifying bacteria protective layer can be formed at the bottom of the net cage, and the bottom of the net cage can be effectively prevented from blackening and smelling after being used regularly. And harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxins and the like can be effectively adsorbed, the pH value of the water body is stabilized, trace elements are supplemented, the transparency of the water body is improved, and the purposes of improving the substrate and purifying the water quality are achieved. The problem of reservoir, large water face net cage breed such as lake, the difficult processing is worsened to net cage bottom environment is solved.

Example two: a dictyoshinium bacteria comprises 2% of liquid bacillus, 50% of bentonite, 52% of silicate, 5% of sodium percarbonate, 1% of proteolytic enzyme and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate.

[ physicochemical Properties ] yellow-white granules.

[ active ingredient content ] Probiotics 10 x 10^8cfu/g

[ DOSAGE ] can be directly and uniformly thrown by dry method.

[ PACKAGE ] 5 kg/bag.

[ STORAGE ] is preserved in a cool, dry and ventilated place for 24 months.

A preparation method of dictyostelium sp comprises the following steps:

Example three: a strain for cleaning the bottom of a net comprises 0.6% of liquid bacillus, 52% of bentonite, 52% of silicate, 4% of sodium percarbonate, 1.4% of proteolytic enzyme and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate.

[ physicochemical Properties ] yellow-white granules.

[ active ingredient content ] Probiotics 10 x 10^8cfu/g

[ DOSAGE ] can be directly and uniformly thrown by dry method.

[ PACKAGE ] 5 kg/bag.

[ STORAGE ] is preserved in a cool, dry and ventilated place for 24 months.

A preparation method of dictyostelium sp comprises the following steps:

Claims

1. Application of bacillus in dredging of the bottom of an aquaculture net cage.

2. The net bottom bacterium cleaning method is characterized by comprising the following steps: comprises 0.6 to 2.5 percent of liquid bacillus, 35 to 53 percent of bentonite, 35 to 53 percent of silicate, 2 to 6 percent of sodium percarbonate, 0.5 to 1.5 percent of protease and the balance of normal saline, wherein the liquid bacillus is filled in the silicate, and the bentonite is coated outside the silicate.

3. The reticulum cleaner according to claim 2, wherein: the liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus contains more than 100 hundred million live bacteria.

4. The reticulum cleaner according to claim 3, wherein: the applicable temperature is 10-40 ℃, and the pH value of the applicable water body is 6.0-10.0.

5. The reticulum cleaner according to claim 4, wherein: the bacteria are cleared by using 40g of net bottom per square meter of water body and are thrown once every 7 to 10 days.

6. A preparation method applied to the dictyostelium sp of any one of claims 2-5, comprising the following steps: