CN112545911A

CN112545911A - Compositions, care agents, uses and methods for reducing skin irritation

Info

Publication number: CN112545911A
Application number: CN202011550164.3A
Authority: CN
Inventors: 黄晨; 瞿怡宁; 吴越; 庄洁; 李慧良
Original assignee: Bloomage Biotech Co Ltd
Current assignee: Bloomage Biotech Co Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2021-03-26
Anticipated expiration: 2040-12-24
Also published as: CN112545911B

Abstract

The present invention provides a composition for reducing skin irritation, comprising ectoin and a cosmetic external preparation, wherein the cosmetic external preparation contains an anionic surfactant. The composition can reduce the damage of the anionic surfactant to the skin barrier, has better effect than that of the cleaning product containing the anionic surfactant and more obvious effect on the weakly acidic cleaning product because the ectoin is directly added.

Description

Compositions, care agents, uses and methods for reducing skin irritation

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a composition, a care agent, application and a method for reducing skin irritation.

Background

The human skin serves as a natural barrier between the body and the environment, which helps the body to resist irritants from the outside, and simultaneously, the skin itself can generate some dirt, such as grease, sweat and the like, but the substances have certain skin moistening and weak antibacterial functions and are also important components of the skin stratum corneum, but a large amount of substances accumulated on the skin surface can cause blockage of skin pores, water and oil balance disorder and the like to generate a series of skin problems, and proper cleaning is necessary.

Anionic surfactants are a large class of surfactants, and are mainly classified into four classes of carboxylates, sulfonates, sulfate salts and phosphate salts. The cleaning product containing the anionic surfactant has the characteristics of high and fine foam, strong dirt-removing power, easiness in cleaning and the like, has certain irritation to the skin after long-term use, and can cause a series of skin barrier problems such as dryness, peeling, pruritus and the like of the skin due to the interaction of the surfactant and protein and lipid in the horny layer.

The reason that cleansing products cause skin barrier problems is due to the interaction of surfactants with the skin, the strongest adsorption of skin molecules by surfactants occurs before the Critical Micelle Concentration (CMC) is reached, and lowering the CMC reduces skin barrier problems caused by cleansing the skin. At present, the surface active agent improves the formula performance and reduces the product irritation through compounding, such as compounding between the surface active agent and the surface active agent, inorganic matters, high polymers, and the like. Through compounding, the repulsion among polar heads is reduced, the mutual attraction of molecules in the micelle adsorption layer is increased, micelle is formed, the adsorption layer is more compact, and the CMC in a formula system is lower. After the CMC is exceeded, the surfactant micelles formed in the cleaning product solution have a significantly reduced irritation. However, the surfactant monomers released from the micelle structure may also cause irritation. Thus, there is currently no particularly effective solution for reducing surfactant irritancy.

Disclosure of Invention

In view of the above-mentioned problems with the prior art, the present invention provides a composition, product and method for reducing skin irritation. Specifically, the present invention relates to the following aspects:

1. a composition for reducing skin irritation, comprising ectoin and a cosmetic external preparation, wherein the cosmetic external preparation contains an anionic surfactant.

2. Composition according to item 1, characterized in that the content by mass of ectoin in the composition is between 0.1% and 10%, preferably between 0.1% and 5%.

3. The composition according to item 1, wherein the anionic surfactant is one or more selected from the group consisting of sodium laureth sulfate, sodium cocoyl glutamate, sodium secondary lauryl alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecylbenzenesulfonate and sodium fatty alcohol-polyoxyethylene ether sulfate.

4. The composition according to item 1, wherein the cosmetic preparation for external use is weakly acidic, preferably has a pH of 5.0 to 6.5.

5. A skin irritation reducing agent comprising a first agent and a second agent, wherein the first agent is a solution containing ectoin, and the second agent is a cosmetic preparation for external use containing an anionic surfactant.

6. The care agent according to item 5, characterized in that the mass content of ectoin in the solution containing ectoin is 0.1% to 10%, preferably 0.1% to 5%.

7. The care agent according to item 5, wherein the anionic surfactant is one or more selected from the group consisting of sodium laureth sulfate, sodium cocoyl glutamate, sodium secondary lauryl alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecyl benzene sulfonate and sodium fatty alcohol polyoxyethylene ether sulfate.

8. The care agent according to item 5, wherein the cosmetic preparation for external use is weakly acidic, preferably has a pH of 5.0 to 6.5.

9. A method for reducing skin irritation, which comprises treating the skin with a solution containing ectoin before using a cosmetic external preparation containing an anionic surfactant.

10. The method according to item 9, characterized in that the mass content of ectoin in the solution comprising ectoin is 0.1% to 10%, preferably 0.1% to 5%.

11. The method according to item 9, characterized in that the cosmetic external preparation and the solution containing ectoin are used at an interval of 0 to 24 hours, preferably 0 to 12 hours, and further preferably 0 to 6 hours.

12. The method according to item 9, wherein the anionic surfactant is one or more selected from the group consisting of sodium laureth sulfate, sodium cocoyl glutamate, sodium secondary lauryl alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecylbenzenesulfonate and sodium fatty alcohol-polyoxyethylene ether sulfate.

13. The method according to item 9, wherein the cosmetic preparation for external use is weakly acidic, preferably has a pH of 5.0 to 6.5.

The invention has the following beneficial effects: before the product containing the anionic surfactant is used, a certain amount of ectoin is used, so that the damage of the anionic surfactant to the skin barrier can be reduced, the effect of the product is better than that of the product containing the anionic surfactant which is directly added into the cleaning product containing the anionic surfactant, and the effect of the product on the weakly acidic cleaning product is more obvious.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be purely exemplary of the invention and are not intended to be limiting.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in experimental or practical applications, the materials and methods are described below. In case of conflict, the present specification, including definitions, will control, and the materials, methods, and examples are illustrative only and not intended to be limiting. The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.

The present invention provides a composition for reducing skin irritation, comprising ectoin and a cosmetic external preparation, wherein the cosmetic external preparation contains an anionic surfactant.

Among them, ectoin is an amphoteric rare amino acid, i.e., 1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid, produced by bacteria in extreme environments in life. Ectoine is the most widely distributed compatible solute in the bacterial community discovered at present, is compatible with the metabolism in cells, does not influence the biomacromolecule function or physiological function of the cells, and is an important osmotic pressure compensation solute. It is reported in the literature that ectoin has the effects of protecting epithelial cells, stabilizing cell membranes, moisturizing skin and reducing the generation of inflammation. The invention creatively discovers that ectoin can improve the immune protection capability of skin cells, increase the cell repair capability and resist external stimulation, particularly the skin barrier problem caused by a surfactant.

The cosmetic preparation for external use refers to a product which is spread on any part of the surface of the human body, such as skin, hair, nails, lips, and teeth, by a painting, spraying, or other similar method, to clean, maintain, beautify, modify, and change the appearance, or to correct the smell of the human body. The product form of the cosmetic external agent is not particularly limited as long as it contains a surfactant, and may be determined according to the use condition, such as cleansing cream, essence, lotion, cream, lotion (such as smoothing toner, toner), mask, makeup remover, gel, aerosol, spray, etc., and the preparation method thereof is a conventional preparation method in the art.

Further, the skin external preparation may further comprise one or more of a thickener, a solubilizer, a cosolvent, a stabilizer, a softener, an aerosol solvent, a preservative, a controlled-release agent, a sustained-release agent, a fragrance, a toner, a penetration enhancer, a humectant, an emulsifier, a shaping agent, or a pearlizing agent.

In a particular embodiment, the composition may comprise ectoin in an amount of 0.1% to 10% by weight, for example 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, preferably 0.1% to 5%.

Surfactants (surfactants) are substances that can significantly reduce the surface tension of a target solution. Has fixed hydrophilic and lipophilic groups and can be directionally arranged on the surface of the solution. The molecular structure of the surfactant has an amphoteric nature: one end is a hydrophilic group, and the other end is a hydrophobic group; the hydrophilic group is often a polar group, such as carboxylic acid, sulfonic acid, sulfuric acid, amino or amino groups and salts thereof, hydroxyl, amide, ether linkages, and the like may also be used as the polar hydrophilic group; and the hydrophobic group is often a non-polar hydrocarbon chain, such as a hydrocarbon chain of 8 or more carbon atoms. The surfactant is divided into ionic surfactant (including cationic surfactant and anionic surfactant), nonionic surfactant, amphoteric surfactant, compound surfactant, other surfactants, etc. Anionic surfactants are the most well developed, largest yielding and most diverse class of surfactants. The partially negatively charged surfactants that function as surface active agents upon ionization in water are referred to as anionic surfactants. Anionic surfactants can be structurally classified into four major classes, carboxylate, sulfonate, sulfate and phosphate.

In a specific embodiment, the anionic surfactant is selected from one or more of sodium laureth sulfate, sodium cocoyl glutamate, sodium lauryl secondary alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecyl benzene sulfonate and sodium fatty alcohol polyoxyethylene ether sulfate.

In a specific embodiment, the cosmetic preparation for external use is weakly acidic, preferably has a pH of 5.0 to 6.5, and may be, for example, 5, 5.5, 6, or 6.5.

The present invention also provides a skin irritation reducing agent comprising a first agent and a second agent, wherein the first agent is a solution containing ectoin and the second agent is a cosmetic external preparation containing an anionic surfactant.

In a specific embodiment of the cosmetic, the external preparation is as defined above.

The solution comprising ectoin may be an aqueous solution, a salt solution, preferably a phosphate buffered solution.

In a specific embodiment, the solution containing ectoin has a mass content of 0.1% to 10%, for example, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, preferably 0.1% to 5%.

The present invention also provides a method for reducing skin irritation, which comprises treating the skin with a solution containing ectoin before using a cosmetic external preparation containing an anionic surfactant. The specific treatment method is not limited, and may be, for example, wiping, soaking, spraying, or the like.

The interval between the use of the cosmetic external preparation and the use of the solution containing ectoin is 0 to 24 hours, preferably 0 to 12 hours, and more preferably 0 to 6 hours.

The present invention can significantly reduce the irritation of an external preparation for skin to the skin by treating the skin with a solution containing ectoin before using the external preparation for cosmetics containing an anionic surfactant. Specifically, according to the dissolution and destructive power tests of protein and lipid, the use of a solution containing 0.1% -10% of ectoin before the use of a cleansing product containing an anionic surfactant can significantly reduce the degree of destruction of the protein and lipid by the cleansing product, particularly, a weakly acidic skin external preparation having a pH value of 5.0-6.5.

Examples

Example 1

A shower gel was prepared as an external preparation for skin, and its formulation is shown in Table 1, and its pH was 5.5. Dissolving a certain amount of ectoin in 1X phosphate buffer solution with pH7.0 to obtain a solution with the mass content of ectoin being 1%. The solution containing ectoin is used before the cosmetic preparation for external use.

TABLE 1

Example 2

A shower gel was prepared as an external preparation for skin, and the pH adjuster shown in Table 1 was adjusted to 6. Dissolving a certain amount of ectoin in 1X phosphate buffer solution with pH7.0 to obtain a solution with 0.1% of ectoin mass content. The solution containing ectoin is used before the cosmetic preparation for external use.

Example 3

A shower gel was prepared as an external preparation for skin, and the pH adjuster in Table 1 was adjusted to 6.5. Dissolving a certain amount of ectoin in 1X phosphate buffer solution with pH7.0 to obtain a solution with the mass content of ectoin being 3%. The solution containing ectoin is used before the cosmetic preparation for external use.

Example 4

The shampoo of table 2 was prepared, and the pH adjuster of table 2 was adjusted to pH 5. Dissolving a certain amount of ectoin in 1X phosphate buffer solution with pH7.0 to obtain a solution with 5% of ectoin mass content. The solution containing ectoin is used before the cosmetic preparation for external use.

TABLE 2

Example 5

The hand sanitizer of table 3 was formulated to have a pH of 5.5. Dissolving a certain amount of ectoin in 1X phosphate buffer solution with pH7.0 to obtain a solution with the mass content of ectoin being 10%. The solution containing ectoin is used before the cosmetic preparation for external use.

TABLE 3

Comparative example 1

The body wash and the solution containing ectoin formulated in comparative example 1 were the same as in example 1 except that ectoin was added to the cosmetic external preparation for use.

Comparative example 2

Comparative example 1 differs from example 1 in that the bath cream prepared was adjusted to have a pH of 8, and the pH adjuster in table 1 was adjusted to have a pH of 8.

Comparative example 3

Comparative example 3 differs from example 1 in that the sodium laureth sulfate of the body wash in table 1 was replaced by an alkyl glycoside having a content of 8%.

Comparative example 4

Comparative example 4 is different from example 1 in that the concentration of the solution containing ectoin is 0. The phosphate buffer solution with pH of 1X 7.0 is applied before cosmetic external preparation.

Comparative example 5

Comparative example 5 is different from example 4 in that the concentration of the solution containing ectoin is 0. The phosphate buffer solution with pH of 1X 7.0 is applied before cosmetic external preparation.

Comparative example 6

Comparative example 6 is different from example 5 in that the concentration of the solution containing ectoin is 0. The phosphate buffer solution with pH of 1X 7.0 is applied before cosmetic external preparation.

The main parameters and adjustments for the specific examples and comparative examples described above are shown in table 4.

TABLE 4

Test examples

Since surfactants interact directly with the proteins and lipids of skin molecules to affect the underlying skin mechanisms, the irritation of the cleansing product was evaluated by the solubilization, destruction potential of the proteins and lipids, respectively.

Test example 1 solubilization and disruption potential of proteins

The potential for solubilization, destruction of proteins was determined by the Zein (Zein) test. Zein is a corn protein which is not dissolved in water, corn-derived Zein is a model protein, and can be denatured after the Zein reacts with a surfactant in a cleaning product so as to be dissolved in a cleaning product solution system, and the dissolution of the Zein in the cleaning product solution is used as an index of the denaturation potential of the surfactant protein and is related to the skin irritation potential of the surfactant. The zein powder was mixed with a 5 wt% cosmetic external agent solution for 24 hours, and then filtered using a nylon membrane, dissolved zein was separated from undissolved substances, and the concentration of zein in the solution was determined using UV absorbance. A greater concentration indicates a greater potential for solubilization and destruction (denaturation) of the protein by the cleaning product.

The experimental method comprises the following steps:

step 1: respectively taking 10ml of the solution containing the ectoin in the above examples and comparative examples, adding 0.1g of zein, stirring for 1 hour at 25 ℃, centrifuging, removing 9ml of supernatant, and respectively standing for 0 hour, 3 hours, 6 hours, 12 hours and 24 hours;

step 2: the external preparations for skin in the above examples and comparative examples were formulated into aqueous solutions of 5 wt% by mass, respectively;

and step 3: and (3) adding 9ml of the solution prepared in the step (2) into the solution prepared in the step (1) respectively, and stirring for 24 hours at 25 ℃.

And 4, step 4: filtering the solution, diluting the filtrate by 25 times with phosphate buffer solution, and measuring absorbance value at 280nm by using a double-beam ultraviolet spectrophotometer (UV-2600, Shimadzu), wherein the absorbance value is shown in Table 5.

TABLE 5 Absorbance value results for examples and comparative examples

Where \ indicates that it cannot be measured. This is because comparative example 1 directly prepared ectoin into the skin external preparation, ectoin and the skin external preparation were used in mixture, other examples and comparative examples were the ectoin solution and the skin external preparation were used separately, 0 to 24 hours were the pretreatment time of the ectoin solution, no pretreatment time was present in the mixed use, and no subsequent value could be measured.

The results show that the protein damage degree of the cleaning product can be reduced by using a cleaning product containing 0.1-10% of ectoine solution before using the cleaning product containing the anionic surfactant or directly adding 0.1-10% of ectoine into the formulation of the cleaning product containing the anionic surfactant for 0-24 hours; however, the use effect of the solution containing the ectoin on protein protection before the cleaning product containing the anionic surfactant is better than that of the cleaning product containing the anionic surfactant.

The results of comparative example 2 show that the effect of using the solution containing ectoin on protein protection before the anionic surfactant cleansing product in an alkaline environment is not significant, but both are effective for the anionic surfactant cleansing product in a weakly acidic environment.

Comparative example 3 the results show that the effect of using the solution containing ectoin on protein protection prior to the amphoteric surfactant containing cleansing product is not significant, but is effective for anionic surfactant cleansing products.

Test example 2 lipid solubilization and destruction potential

The solubilization, destruction potential of lipids was determined by the Corneosurfametry test. The Corneosporatry test method is a quantitative visualization method for rapidly, non-invasively and in vitro measuring the influence of a cleaning product on the actual human stratum corneum. The method used is disclosed in Modified cardiovascular Science as a new acceptable high-throughput visual method for predicting clinical effects twards human skin, International Journal of Cosmetic Science,2016,38, 178-. Sticking D-Squame adhesive tape on forearm skin of subject, extracting horny layer, soaking horny layer adhesive tape samples in 10 wt% cleaning product for 10min, taking out, washing with running water for 1min, and air drying for 30 min. Dyeing with basic fuchsin-toluidine blue ethanol mixed solution for 3min, taking out, washing with flowing water for 1min, and air drying for 30 min. The product with strong irritation has dark dyeing color, and the product with mild irritation has light dyeing color. The LAB value of each tape sample was measured by a colorimeter (US-400 colorimeter) and the stratum corneum surface irritation index (IOI) was calculated by a formula, the higher the IOI, the worse the product mildness. The formula is as follows:

color C^*The value: c^*＝(a^*2+b^*2)^1/2

Cuticle surface mildness index: CIM (Corneosporadic index of miless) ═ L^*-C^*

Stratum corneum surface irritation index (IOI): IOI (index of irritation) ═ 1- (CIM)_testproduct/CIM_water)

The experimental method comprises the following steps:

the forearms of healthy subjects (aged 18-65 years) were cleaned and then placed in a constant temperature and humidity room for 30min, the horny layer was extracted with D-Squame tape, the first layer was discarded and the second layer was kept for future use.

Step 1: soaking the adhesive tape samples in the solutions containing the ectoin of the examples and the comparative examples for 1h, taking out and respectively standing for 0h, 3h, 6h, 12h and 24 h;

step 2: soaking the adhesive tape sample in 10 wt% cosmetic external agent water solution for 10min, taking out, washing with flowing water for 1min, and air drying for 30 min;

and step 3: dyeing with basic fuchsin-toluidine blue ethanol mixed solution for 3min, taking out, washing with flowing water for 1min, and air drying for 30 min.

And 4, step 4: LAB values of the tape samples after dyeing were measured with a colorimeter, and a formula was calculated to obtain the stratum corneum surface irritation index (IOI), and the obtained results are shown in table 6.

TABLE 6 results of surface irritation index for each of the examples and comparative examples

Wherein the "\ indicates that measurement is impossible. This is because comparative example 1 directly prepared ectoin into the skin external preparation, ectoin and the skin external preparation were used in mixture, other examples and comparative examples were the ectoin solution and the skin external preparation were used separately, 0 to 24 hours were the pretreatment time of the ectoin solution, no pretreatment time was present in the mixed use, and no subsequent value could be measured.

The results show that the use of a cleaning product containing 0.1-10% of ectoin solution before the use of the cleaning product containing the anionic surfactant or the addition of 0.1-10% of ectoin to the formulation of the cleaning product containing the anionic surfactant for 0-24 hours can reduce the damage degree of the cleaning product to the lipid; however, the use effect of the solution containing ectoin on lipid protection before the use of the cleaning product containing the anionic surfactant is better than that of the cleaning product containing the anionic surfactant.

The results of comparative example 2 show that the effect of lipid protection using the solution containing ectoin before the anionic surfactant cleansing product in an alkaline environment is not significant, but both are effective for the anionic surfactant cleansing product in a weakly acidic environment.

Comparative example 3 the results show that the effect of using the solution containing ectoin prior to the amphoteric surfactant containing cleansing product on lipid protection is not significant, but is effective for anionic surfactant cleansing products.

Claims

2. Composition according to claim 1, characterized in that the content by mass of ectoine in the composition is between 0.1% and 10%, preferably between 0.1% and 5%.

3. The composition of claim 1, wherein the anionic surfactant is selected from one or more of sodium laureth sulfate, sodium cocoyl glutamate, sodium secondary lauryl alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecyl benzene sulfonate, and sodium fatty alcohol polyoxyethylene ether sulfate.

4. The composition according to claim 1, wherein the cosmetic preparation for external use is weakly acidic, preferably has a pH of 5.0 to 6.5.

6. The agent according to claim 5, characterized in that the content by mass of ectoin in the solution containing ectoin is between 0.1% and 10%, preferably between 0.1% and 5%.

7. The care agent as claimed in claim 5, wherein the anionic surfactant is one or more selected from sodium laureth sulfate, sodium cocoyl glutamate, sodium secondary lauryl alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecyl benzene sulfonate and sodium fatty alcohol polyoxyethylene ether sulfate.

8. The care agent according to claim 5, wherein the cosmetic preparation for external use is weakly acidic, preferably has a pH value of 5.0 to 6.5.

10. Method according to claim 9, characterized in that the mass content of ectoin in the solution comprising ectoin is between 0.1% and 10%, preferably between 0.1% and 5%.