CN112545911B

CN112545911B - Compositions, care agents, uses and methods for reducing skin irritation

Info

Publication number: CN112545911B
Application number: CN202011550164.3A
Authority: CN
Inventors: 黄晨; 瞿怡宁; 吴越; 庄洁; 李慧良
Original assignee: Bloomage Biotech Co Ltd
Current assignee: Bloomage Biotech Co Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2023-06-20
Anticipated expiration: 2040-12-24
Also published as: CN112545911A

Abstract

The present invention provides a composition for reducing skin irritation comprising ectoine and a cosmetic external preparation, wherein the cosmetic external preparation contains an anionic surfactant. The composition can reduce the damage of the anionic surfactant to the skin barrier, has better effect than that of directly adding the ectoin into the cleaning product containing the anionic surfactant, and has more obvious effect on the slightly acidic cleaning product.

Description

Compositions, care agents, uses and methods for reducing skin irritation

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a composition for reducing skin irritation, a nursing agent, application and a method.

Background

The human skin serves as a natural barrier between the body and the environment, and helps the body resist the stimulus from the outside, and meanwhile, the skin itself can generate some dirt such as grease, sweat and the like, but the substances have certain skin moisturizing and weak antibacterial functions and are important components of the skin stratum corneum, but a large amount of accumulation on the skin surface can cause skin pore blockage, water-oil balance disorder and the like to generate a series of skin problems, and proper cleaning is necessary.

Anionic surfactants are a broad class of surfactants and are largely divided into carboxylate, sulfonate, sulfate and phosphate groups. The cleaning product containing the anionic surfactant has the characteristics of high and fine foam, strong detergency, easiness in cleaning and the like, has certain irritation to skin after long-term use, and can interact with proteins and lipids in the stratum corneum to cause a series of skin barrier problems such as dryness, flaking, itching and the like of the skin.

The skin barrier problem caused by cleansing products is due to the interaction of the surfactant with the skin, the strongest adsorption of the surfactant to skin molecules occurring before the Critical Micelle Concentration (CMC) is reached, lowering CMC can reduce the skin barrier problem caused by cleansing the skin. At present, the surfactant improves the formula performance and reduces the product irritation through compounding, such as compounding between the surfactant and the surfactant, inorganic matters, high polymers and the like. The repulsion between polar heads is reduced through compounding, the mutual attraction of molecules in the micelle adsorption layer is increased, micelle is formed, the adsorption layer is more compact, and CMC in a formula system is lower. After exceeding CMC, the surfactant micelles formed in the cleaning product solution have significantly reduced irritation. However, the surfactant monomers released from the micelle structure may also cause irritation. Thus, there is currently no particularly effective solution to reduce the irritation of surfactants.

Disclosure of Invention

In view of the foregoing problems with the prior art, the present invention provides a composition, product and method for reducing skin irritation. In particular, the invention relates to the following aspects:

1. a composition for reducing skin irritation comprising ectoine and a cosmetic external preparation, wherein the cosmetic external preparation comprises an anionic surfactant.

2. Composition according to claim 1, characterized in that the content of ectoine in the composition is 0.1-10% by mass, preferably 0.1-5% by mass.

3. The composition according to claim 1, wherein the anionic surfactant is one or more selected from sodium laureth sulfate, sodium cocoyl glutamate, sodium laurylsulfonate, sodium cocoyl methyltaurine, sodium dodecylbenzenesulfonate and sodium fatty alcohol polyoxyethylene ether sulfate.

4. The composition according to claim 1, wherein the cosmetic external preparation is weakly acidic, preferably at a pH of 5.0-6.5.

5. A skin irritation reducing care agent comprising a first agent and a second agent, wherein the first agent is a solution comprising ectoin and the second agent is a cosmetic external agent comprising an anionic surfactant.

6. The care agent according to claim 5, characterized in that the mass content of exendin in the solution comprising exendin is 0.1-10%, preferably 0.1-5%.

7. The care agent according to claim 5, wherein the anionic surfactant is one or more selected from sodium laureth sulfate, sodium cocoyl glutamate, sodium laurylsulfonate, sodium cocoyl methyltaurine, sodium dodecylbenzenesulfonate and sodium fatty alcohol polyoxyethylene ether sulfate.

8. The care agent according to claim 5, wherein the cosmetic external preparation is weakly acidic, preferably at a pH of 5.0 to 6.5.

9. A method of reducing skin irritation comprising treating skin with a solution comprising ectoin prior to use of a cosmetic external preparation comprising an anionic surfactant.

10. The method according to claim 9, characterized in that the content of ectoin in the ectoin-containing solution is 0.1% -10%, preferably 0.1% -5% by mass.

11. The method according to claim 9, wherein the cosmetic external preparation and the solution comprising ectoin are used at an interval of 0 to 24 hours, preferably 0 to 12 hours, and more preferably 0 to 6 hours.

12. The method according to claim 9, wherein the anionic surfactant is one or more selected from sodium laureth sulfate, sodium cocoyl glutamate, sodium laurylsulfonate, sodium cocoyl methyltaurine, sodium dodecylbenzenesulfonate, and sodium fatty alcohol polyoxyethylene ether sulfate.

13. The method according to claim 9, wherein the cosmetic external preparation is weakly acidic, preferably at a pH of 5.0-6.5.

The invention has the following beneficial effects: before the product containing the anionic surfactant is used, a certain amount of ectoin is used, so that the damage of the anionic surfactant to the skin barrier can be reduced, the effect is better than that of directly adding ectoin into the cleaning product containing the anionic surfactant, and the effect is more obvious for the slightly acidic cleaning product.

Detailed Description

The invention will be further illustrated with reference to the following examples, which are to be understood as merely further illustrating and explaining the invention and are not to be construed as limiting the invention.

Unless defined otherwise, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the materials and methods are described herein below. In case of conflict, the present specification, including definitions therein, will control and materials, methods, and examples, will control and be in no way limiting. The invention is further illustrated below in connection with specific examples, which are not intended to limit the scope of the invention.

The invention provides a composition for reducing skin irritation, which is characterized by comprising ectoine and a cosmetic external preparation, wherein the cosmetic external preparation contains an anionic surfactant.

Among them, escidodine is an amphoteric rare amino acid, namely 1,4,5, 6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, produced by bacteria in extreme environments in life. The ectoine is the most widely-distributed compatible solute in the bacterial kingdom, is compatible with metabolism in cells, does not affect the biological macromolecular function or the physiological function of the cells, and is an important osmotic pressure compensation solute. Literature data report that ectoin has the effects of protecting epithelial cells, stabilizing cell membranes, moisturizing skin and reducing inflammation. The creative discovery of the invention is that the ectoin can improve the skin cell immunity protection capability and the cell repair capability, and can resist external stimulus, especially the skin barrier problem caused by the surfactant.

The cosmetic external preparation is applied, sprayed or the like to any part of the surface of the human body, such as skin, hair, nails, lips and teeth, etc., to achieve a product for cleaning, caring, beautifying, modifying and changing the appearance, or correcting the smell of the human body. The product form of the cosmetic external agent is not particularly limited as long as the cosmetic external agent contains a surfactant, and can be determined according to the use condition, such as face cream, essence, emulsion, cream, toning lotion (such as skin softening lotion and skin refreshing lotion), mask, makeup remover, gel, aerosol or spray and the like, and the preparation method is a preparation method conventional in the field.

Further, the skin external agent may further contain one or more of a thickener, a solubilizer, a cosolvent, a stabilizer, a softener, an aerosol solvent, a preservative, a controlled release agent, a sustained release agent, a fragrance, a toner, a permeation enhancer, a humectant, an emulsifier, a shaping agent, or a pearlizing agent.

In a specific embodiment, the content of the ectoin in the composition is 0.1% -10% by mass, for example, may be 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, preferably 0.1% -5%.

A surfactant (surfactant) is a substance that significantly reduces the surface tension of a target solution. Has immobilized hydrophilic and lipophilic groups, and can be oriented on the surface of the solution. The molecular structure of the surfactant has amphoteric properties: one end is hydrophilic group, and the other end is hydrophobic group; the hydrophilic group is usually a polar group such as carboxylic acid, sulfonic acid, sulfuric acid, amino or amine group and salts thereof, and hydroxyl group, amide group, ether bond and the like can also be used as the polar hydrophilic group; while hydrophobic groups are often nonpolar hydrocarbon chains, such as hydrocarbon chains of more than 8 carbon atoms. Surfactants are classified into ionic surfactants (including cationic surfactants and anionic surfactants), nonionic surfactants, amphoteric surfactants, built surfactants, other surfactants, and the like. Anionic surfactants are the most developed, most productive and most varied products of surfactants. The partially negatively charged surfactant that acts as a surfactant after ionization in water is referred to as an anionic surfactant. Anionic surfactants can be structurally classified into four general classes of carboxylate, sulfonate, sulfate and phosphate.

In a specific embodiment, the anionic surfactant is selected from one or more of sodium laureth sulfate, sodium cocoyl glutamate, sodium lauroyl Gui Jizhong alkyl sulfonate, sodium cocoyl methyl taurate, sodium dodecyl benzene sulfonate, and sodium fatty alcohol polyoxyethylene ether sulfate.

In a specific embodiment, the cosmetic external preparation is weakly acidic, preferably has a pH of 5.0 to 6.5, and may be, for example, 5, 5.5, 6, 6.5.

The invention also provides a care agent for reducing skin irritation, which comprises a first agent and a second agent, wherein the first agent is a solution containing the ectoin, and the second agent is a cosmetic external agent containing an anionic surfactant.

In particular embodiments of the cosmetic, the external agent is defined as described above.

The solution containing the ectoin may be an aqueous solution, a salt solution, and preferably a phosphate buffer solution.

In a specific embodiment, the content of the exendin in the solution containing the exendin is 0.1% -10%, for example, may be 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, and preferably 0.1% -5%.

The present invention also provides a method of reducing skin irritation comprising treating skin with a solution comprising exendin prior to the use of a cosmetic external agent comprising an anionic surfactant. The specific treatment mode is not limited, and may be wiping, soaking, spraying, etc.

The interval between the use of the cosmetic external preparation and the use of the solution containing ectoin is 0 to 24 hours, preferably 0 to 12 hours, and more preferably 0 to 6 hours.

The present invention can significantly reduce the skin irritation of a skin external preparation by treating the skin with a solution containing exendin before using a cosmetic external preparation containing an anionic surfactant. Specifically, according to the solubilization and destructiveness test of proteins and lipids, the use of a solution containing 0.1% -10% of ectoin prior to the use of a cleansing product containing an anionic surfactant can significantly reduce the degree of destructiveness of the cleansing product to proteins and lipids, especially for weakly acidic skin external preparations having a pH of 5.0-6.5.

Examples

Example 1

The formulation of the bath lotion as an external skin preparation is shown in table 1, and the pH of the bath lotion is 5.5. A certain amount of exendin is dissolved in 1X phosphate buffer with pH7.0 to obtain a solution with the mass content of 1% of exendin. The use of the solution comprising ectoine is prior to the cosmetic preparation for external use.

TABLE 1

Example 2

The bath lotion was prepared as a skin external preparation, and the pH adjuster of table 1 was adjusted to pH 6. A certain amount of exendin is dissolved in 1X phosphate buffer with pH7.0 to obtain a solution with the mass content of 0.1 percent of exendin. The use of the solution comprising ectoine is prior to the cosmetic preparation for external use.

Example 3

The bath lotion was prepared as a skin external preparation, and the pH adjuster of table 1 was adjusted to pH 6.5. A certain amount of exendin is dissolved in 1X phosphate buffer with pH7.0 to obtain a solution with the mass content of 3 percent of exendin. The use of the solution comprising ectoine is prior to the cosmetic preparation for external use.

Example 4

The shampoo of table 2 was prepared, and the pH adjuster of table 2 was adjusted to pH 5. A certain amount of exendin is dissolved in 1X phosphate buffer with pH7.0 to obtain a solution with 5% of exendin mass content. The use of the solution comprising ectoine is prior to the cosmetic preparation for external use.

TABLE 2

Example 5

The hand sanitizer of table 3 was formulated to have a pH of 5.5. A certain amount of exendin is dissolved in 1X phosphate buffer with pH7.0 to obtain a solution with the mass content of 10% of exendin. The use of the solution comprising ectoine is prior to the cosmetic preparation for external use.

TABLE 3 Table 3

Comparative example 1

The body wash prepared in comparative example 1 was identical to the solution containing exendin as in example 1, except that exendin was added to the cosmetic preparation for external use.

Comparative example 2

Comparative example 1 was different from example 1 in that the pH of the prepared body wash was 8, and the pH adjustor of table 1 was adjusted to pH 8.

Comparative example 3

Comparative example 3 differs from example 1 in that the sodium laureth sulfate of the body wash in table 1 was exchanged for an alkyl glycoside with a content of 8%.

Comparative example 4

Comparative example 4 differs from example 1 in that the concentration of the solution comprising exendin is 0. The phosphate buffer of pH7.0 at 1X is used before the cosmetic preparation for external use.

Comparative example 5

Comparative example 5 differs from example 4 in that the concentration of the solution comprising exendin is 0. The phosphate buffer of pH7.0 at 1X is used before the cosmetic preparation for external use.

Comparative example 6

Comparative example 6 differs from example 5 in that the concentration of the solution comprising exendin is 0. The phosphate buffer of pH7.0 at 1X is used before the cosmetic preparation for external use.

The main parameters and adjustments of the specific examples and comparative examples described above are shown in table 4.

TABLE 4 Table 4

Test examples

Since surfactants interact directly with proteins and lipids of skin molecules to affect the underlying skin mechanisms, the irritation potential of the cleaning product is assessed by the potential for solubilization and destruction of the proteins and lipids, respectively.

Test example 1 potential for protein solubilization and destruction

The potential for protein solubilization and disruption was determined by the Zein (Zein) assay. Zein is a self-insoluble corn protein, zein from corn is a model protein that denatures when it reacts with surfactants in the cleaning product, causing it to dissolve in the cleaning product solution system, and Zein dissolution in the cleaning product solution is used as a potential indicator of surfactant protein denaturation and is related to the skin irritation potential of the surfactant. The zein powder was mixed with 5wt% cosmetic topical solution for 24 hours, then filtered using nylon membrane, the dissolved zein was separated from undissolved material, and the concentration of zein in the solution was determined using UV absorbance. The greater the concentration, the greater the potential of the cleaning product to solubilize and destroy (denature) proteins.

The experimental method comprises the following steps:

step 1: taking 10ml of the solution containing the ectoin in the above examples and comparative examples respectively, adding 0.1g of zein, stirring for 1 hour at 25 ℃, centrifuging, removing 9ml of supernatant, and standing for 0h, 3h, 6h, 12h and 24h respectively;

step 2: the external preparations for skin in the above examples and comparative examples were respectively formulated as aqueous solutions having a mass ratio of 5 wt%;

step 3: taking 9ml of the solution prepared in the step 2, adding the 9ml of the solution into the solution prepared in the step 1, and stirring the solution at 25 ℃ for 24 hours.

Step 4: filtering the solution, diluting the filtrate with phosphate buffer solution for 25 times, and measuring absorbance value at 280nm by using a double-beam ultraviolet spectrophotometer (UV-2600, shimadzu), wherein the concentration of zein can be represented, and the absorbance value is shown in Table 5.

Table 5 absorbance value results for each example and comparative example

Wherein \indicates that measurement is impossible. This is because comparative example 1 was prepared by directly adding exendin to an external skin preparation, and exendin and external skin preparation were mixed for use, and other examples and comparative examples were prepared by separately adding exendin solution and external skin preparation, and 0 to 24 hours were pretreatment time of exendin solution, and no pretreatment time was present for mixed use, and subsequent values could not be measured.

The results show that the use of a cleaning product formulation containing 0.1% to 10% of the ectoin solution prior to the use of a cleaning product containing anionic surfactant or the addition of 0.1% to 10% of ectoin directly to the anionic surfactant for 0 to 24 hours can reduce the degree of protein damage from the cleaning product; however, the use of solutions containing exendin prior to the use of anionic surfactant-containing cleaning products has been shown to be superior to the use of anionic surfactant-containing cleaning products for protein protection.

The results of comparative example 2 show that the use of the solution containing ectoin prior to the anionic surfactant cleaning product in alkaline environment is not significant in protein protection, but is effective for the anionic surfactant cleaning product in weak acidic environment.

Comparative example 3 the results show that the use of solutions containing ectoin prior to cleaning products containing amphoteric surfactants is not significant in protein protection and is effective for anionic surfactant cleaning products.

Test example 2 potential for lipid dissolution and destruction

The potential for dissolution and destruction of lipids was determined by the Corneofarmetry assay. The Corneosuraframe test method is a quick, noninvasive and in-vitro quantitative visualization method for determining the influence of a cleaning product on the stratum corneum of an actual human body. Methods employed are disclosed in Modified Corneosurfametry as a new accelerated high-throughputex vivomethodology for predicting cleanser effects towards human skin, international Journal of Cosmetic Science,2016,38,178-186. The D-Squame tape was applied to the forearm skin of the subject, the stratum corneum was extracted, the stratum corneum tape samples were each soaked in 10wt% cleaning product for 10min, removed, rinsed with running water for 1min, and air dried for 30min. Dyeing with mixed solution of basic fuchsin-toluidine blue ethanol for 3min, taking out, washing with running water for 1min, and air drying for 30min. The product with strong irritation has dark dyeing color, and the product with mild irritation has light dyeing color. The LAB value of each tape sample was measured by a colorimeter (meridak CR-400 colorimeter) and the formula was calculated to give the stratum corneum surface irritation index (IOI), the higher the IOI, the worse the product mildness. The formula is as follows:

chromaticity C ^* Value: c (C) ^* ＝(a ^*2 +b ^*2 ) ^1/2

Stratum corneum surface mildness index: CIM (Corneosurfametric index of mildness) =l ^* -C ^*

Stratum corneum surface irritation index (IOI): IOI (Index of irritation) =1- (CIM) _testproduct /CIM _water )

The experimental method comprises the following steps:

the forearm of healthy interviewee (18-65 years old) is cleaned and then is rested in a constant temperature and humidity room for 30min, the horny layer is extracted by using D-square adhesive tape, the first layer is removed, and the second layer is reserved.

Step 1: immersing the tape sample in the solution containing the ectoin for 1h in the above examples and comparative examples, taking out and placing for 0h, 3h, 6h, 12h and 24h respectively;

step 2: soaking the adhesive tape sample in 10wt% of cosmetic external agent water solution for 10min, taking out, washing with running water for 1min, and air drying for 30min;

step 3: dyeing with basic fuchsin-toluidine blue ethanol mixed solution for 3min, taking out, washing with running water for 1min, and air drying for 30min.

Step 4: LAB values of the stained tape samples were measured with a colorimeter and the formula was calculated to give the stratum corneum surface irritation index (IOI) with the results shown in table 6.

Table 6 surface stimulation index results for each of the examples and comparative examples

The results show that the use of a cleaning product formulation containing 0.1% to 10% of the ectoin solution prior to the use of a cleaning product containing anionic surfactant or the addition of 0.1% to 10% of ectoin directly to the anionic surfactant for 0 to 24 hours can reduce the degree of damage to the lipids by the cleaning product; however, the use of solutions containing exendin for lipid protection prior to the use of anionic surfactant-containing cleaning products is superior to the use of anionic surfactant-containing cleaning products.

The results of comparative example 2 show that the use of the solution containing ectoin prior to the anionic surfactant cleaning product in alkaline environment is not significant in lipid protection, but is effective for anionic surfactant cleaning products in weak acidic environment.

Comparative example 3 the results show that the lipid protection effect of using the solution containing ectoin prior to the amphoteric surfactant containing cleaning product is not significant, but is effective for the anionic surfactant cleaning product.

Claims

1. A method for reducing skin irritation for non-therapeutic purposes, comprising treating skin with a solution comprising exendin, which is weakly acidic, prior to using a cosmetic external preparation comprising an anionic surfactant, wherein the amount of exendin in the solution comprising exendin is 0.1% to 10% by mass, and wherein the anionic surfactant is one or more selected from sodium laureth sulfate, sodium cocoyl glutamate, sodium laurylsulfonate, sodium cocoyl methylttaurate, sodium dodecylbenzenesulfonate, and sodium fatty alcohol polyoxyethylene ether sulfate.

2. The method according to claim 1, wherein the content of the exendin in the solution comprising the exendin is 0.1-5% by mass.

3. The method according to claim 1, wherein the cosmetic external preparation and the solution containing ectoin are used at an interval of greater than 0 and equal to or less than 24 hours.

4. The method according to claim 3, wherein the cosmetic external preparation and the solution containing ectoin are used at an interval of more than 0 and less than or equal to 12 hours.

5. The method according to claim 4, wherein the cosmetic external preparation and the solution containing ectoin are used at an interval of more than 0 and less than or equal to 6 hours.

6. The method according to claim 1, wherein the cosmetic topical agent has a pH of 5.0 to 6.5.