CN112538444B - Rice washing water fermentation product with scalp oil control and hair softening double-effect functions - Google Patents
Rice washing water fermentation product with scalp oil control and hair softening double-effect functions Download PDFInfo
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Abstract
The invention provides a rice washing water fermentation product with a scalp oil control and hair softening double-effect function, belongs to the technical field of fermentation, and on the one hand, the invention provides a microbial composition for rice washing water fermentation, wherein one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus are contained in the microbial composition, the application of the microbial composition in preparing the rice washing water fermentation product is provided, the microbial composition is applied to the fermentation of rice washing water by controlling the variety and the mass ratio of microorganisms, the content of protein, amino acid, short peptide and the like in the obtained fermentation product is greatly increased, and the rice washing water fermentation product obtained by fermentation in the implementation process is mixed with a surfactant and a plant extract to prepare a shampoo product, so that the shampoo product has the double effects of oil control, itching relieving and softening and brightening the hair.
Description
Technical Field
The invention belongs to the technical field of fermentation, and particularly relates to a rice washing water fermentation product with a scalp oil control and hair softening double-effect function.
Background
The rice is one of the main grain crops in China and the second crop in the world, and the annual output of China exceeds 2.0 hundred million tons. The rice washing water is water left after washing rice, and mainly contains nutritional ingredients of rice bran and aleurone layer, including protein, dietary fiber, starch, oryzanol, vitamins, minerals and other bioactive ingredients. The rice bran protein has anticancer activity and health promotion function, and the rice bran peptide is low molecular weight polypeptide obtained by hydrolyzing rice bran protein with protease, and has low content of polypeptideSensitivity and easy dissolution. There are researchers subjecting rice bran to CO2The extract shows good hair growth effect by supercritical extraction, and the hair follicles are increased remarkably. The rice washing water is put into a basin or a barrel and the like for natural fermentation every day by the people of the Dai nationality in Yunnan, and the traditional fermented rice washing water is used for washing hair and has the effects of removing dandruff, moistening hair, brightening hair, promoting hair to turn black and thick and the like.
Therefore, more and more researches have been made on the use of fermented product of rice washing water in washing and protecting products, such as Chinese patent application 201910611378.8, which discloses a washing product of rice washing water, comprising the following components in parts by weight: 30-40 parts of rice fermentation rice washing water and 30-40 parts of glutinous rice fermentation rice washing water, but the traditional fermentation method is used for fermentation in the fermentation process, fermentation microorganisms are not selected, the obtained fermentation liquor cannot better moisten hair, and the oil control and itching relieving effects are not prominent.
Further, as disclosed in chinese patent application 201610914741.X, a shampoo product composition is disclosed, which comprises the following components in parts by mass: 0.1-5 parts of ginseng extract, 0.1-5 parts of glossy privet fruit extract, 0.1-5 parts of tea seed protein and 0.1-5 parts of pine needle extract. The shampoo product composition is prepared by scientifically compounding the effective proportions, and the glossy privet fruit extract, the ginseng extract, the tea seed protein and the pine needle extract are combined to play roles in nourishing, forming a film, eliminating free radicals, delaying senescence and promoting hair growth, so that the effects of smoothness and brightness are realized from inside to outside. The magnolia bark extract and the houttuynia cordata extract have the obvious effect of inhibiting the growth of gram-positive bacteria such as staphylococcus aureus and fungi such as malassezia, and excessive reproduction of the microorganisms is just the reason of dandruff breeding and dullness. The fermented rice washing water and the ginger extract are combined, so that the effects of supplementing scalp nutrition and promoting metabolism can be achieved, the effects of softening and brightening the composition are further improved, the dual effects of controlling oil and relieving itching and enabling hair to be smooth and glossy cannot be achieved, the fermented rice washing water used by the composition is obtained by natural fermentation through a traditional method, the fermentation effect is poor, and the effect is not obvious.
However, according to the description of the prior art, the conventional strains are used for fermentation when rice washing water is fermented, and although the conventional microorganisms have certain effects in the rice washing water fermentation process, in view of the poor rice washing water fermentation effect of the conventional microorganisms and the single type of microorganisms in the obtained fermented product, it is necessary to screen and optimize the combined microorganisms to enhance the fermentation effect of the microorganisms, and in view of the synergistic effect of the multiple microorganisms which are prepared in a compounding manner and can synergistically enhance the functions of each other or improve the synergistic capability of the microorganisms, so that the preparation has more functions and better effects, and 3 strains with good effects, namely Bifidobacterium longum (Bifidobacterium longum), Lactobacillus acidophilus (Lactobacillus acidophilus) and Pediococcus pentosaceus (Pediococcus pentosaceus), are finally obtained through selection. The results show that the rice washing water is fermented by using the above-mentioned bacteria mixture, and the fermented product is separated, so that the obtained rice washing water fermentation liquor can be used in shampoo products, and can effectively raise the oil-controlling and itching-relieving property of shampoo products, and can make hair smooth and bright.
The applicant group utilizes the optional combination of 3 screened beneficial bacteria (bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus) to prepare a microorganism compound preparation, carries out fermentation treatment on rice washing water, combines with the specific combination proportion of plant extracts and auxiliary materials of shampoo products, observes the fermentation capacity of the microorganism compound preparation rice washing water on the one hand, uses the obtained leavening in the shampoo products on the one hand, carries out product development, respectively obtains shampoo products with different efficacies of various specific compound combination proportions and plant extracts and auxiliary material combination proportions, and respectively carries out patent protection.
Disclosure of Invention
The applicant group of the invention respectively identifies 3 strains of microorganisms, namely pediococcus pentosaceus, lactobacillus acidophilus and bifidobacterium longum, by screening and purifying natural fermentation liquor or purely culturing the 3 strains of microorganisms, and preserves the 3 strains of microorganisms, wherein the preservation information is as follows:
the Pediococcus pentosaceus (Pediococcus pentosaceus) is Pediococcus pentosaceus PP-2020, has a preservation number of CGMCC No.20682, has a preservation time of 09 months and 21 days in 2020, is preserved in China general microbiological culture Collection center (CGMCC), and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus acidophilus LA-2020 with the preservation number of CGMCC No.20683 and the preservation time of 2020, 09 and 21 days, is preserved in China general microbiological culture Collection center (CGMCC) of China Committee for culture Collection of microorganisms, and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Bifidobacterium longum (Bifidobacterium longum) is Bifidobacterium longum BL-2020, the preservation number is CGMCC No.20684, the preservation time is 09/21 days in 2020, and the Bifidobacterium longum is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the Bifidobacterium longum are as follows: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The technical development is carried out on the compound fermentation product of the 3 strains of microorganisms to obtain a fermentation product.
The preparation method of the fermentation product comprises the step of fermenting the mixed culture raw material by using the microbial composition, wherein the mixed culture raw material at least comprises rice washing water.
More specifically, the preparation method of the rice washing water fermentation liquor comprises the following steps:
soaking rice in distilled water, stirring, and filtering to obtain filtrate; putting the filtrate into a closed container, adding the microbial composition, vacuumizing, fermenting, and fermenting to obtain the rice washing water fermentation product.
Centrifuging the obtained rice washing water fermentation product, separating precipitate, repeatedly freezing and thawing the precipitate, centrifuging, drying and pulverizing to obtain filtrate, i.e. rice washing water fermentation lysate.
Filtering the obtained rice washing water fermentation product to obtain filtrate, namely the rice washing water extracellular substances.
Wherein, the mass percent of the rice is 0.5-2%; preferably 1-2%; and more preferably 1%; the stirring speed is 150-250 r/min; preferably 200 r/min; the fermentation temperature is 35-38 ℃; preferably 37 ℃; the fermentation time is 50-70 hours; preferably 60 hours.
The operation steps of the repeated freeze-thaw treatment are as follows: freezing the precipitate at-15 deg.C to-20 deg.C, thawing at room temperature, freezing at-15 deg.C to-20 deg.C, thawing at room temperature, and repeating for 2-3 times;
wherein the mass volume ratio of the microbial composition to the filtrate is 1-5%; preferably 3%.
The weight of thalli in the microbial composition is 0.2-0.4% of the weight of a microbial solution; preferably 0.3%.
The rice is one or more of rice, millet and glutinous rice.
According to the invention, the rice washing water is fermented by using the microbial composition, so that the contents of protein, amino acid, short peptide and the like in a rice washing water fermentation product are greatly improved.
The microbial composition is one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus;
the microorganism composition is pediococcus pentosaceus;
the microbial composition comprises pediococcus pentosaceus and bifidobacterium longum in a mass ratio of 1-2: 1;
the microbial composition is pediococcus pentosaceus and lactobacillus acidophilus in a mass ratio of 1: 1-2.5;
preferably, the microbial composition is a turbid mixture of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus.
Preferably, the ratio of bifidobacterium longum to lactobacillus acidophilus to pediococcus pentosaceus in the microbial composition is 1:2-5: 1-2; preferably 1:3 to 5: 2; still more preferably 1:5: 2.
In the implementation process, the invention unexpectedly discovers that the proportion of three microorganisms, namely bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus, has obvious influence on the components in the fermentation product.
The content of various proteins, amino acids, short peptides and the like in the fermentation product can be obviously improved by controlling the ratio of bifidobacterium longum to lactobacillus acidophilus to pediococcus pentosaceus to be 1:2-5:1-2, and the fermentation liquor obtained by separating the fermentation product is used for mixing the shampoo product with other components, so that the oil control and itching relieving performance of the shampoo product can be improved, and the hair is smooth and bright.
According to the invention, the rice washing water is fermented by the obtained microbial composition through reasonably controlling the mass ratio of the microbes, the obtained rice washing water fermentation liquor contains various proteins, amino acids and short peptides, and the content is higher, so that the application of the rice washing water fermentation liquor to a shampoo product can obviously improve the oil control and itching relieving performance of the shampoo product, and the hair is smooth and bright.
The invention also provides a shampoo product, which contains the rice washing water fermentation product prepared by the method.
The shampoo product has the double effects of controlling oil, relieving itching and enabling hair to be smooth and glossy.
The shampoo product comprises the following components in parts by weight: 15-25 parts of rice washing water fermentation product, 25-40 parts of surfactant, 20-30 parts of plant extract, 5-10 parts of auxiliary agent, 2-5 parts of essence and 15-30 parts of deionized water.
Preferably, the shampoo product comprises the following components in parts by weight: 20-25 parts of rice washing water fermentation product, 30-40 parts of surfactant, 25-30 parts of plant extract, 6-8 parts of auxiliary agent, 3-5 parts of essence and 20-30 parts of deionized water.
Still preferably, the shampoo product comprises the following components in parts by weight: 25 parts of rice washing water fermentation product, 36 parts of surfactant, 32 parts of plant extract, 8 parts of auxiliary agent, 3 parts of essence and 20 parts of deionized water.
The surfactant is one or more of sodium cocoyl glutamate, potassium cocoyl glycinate, sodium lauroyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, lauryl glucoside, decyl glucoside, cocoyl glucoside, fatty alcohol polyoxyethylene ether sulfate and alpha-alkenyl sulfonate;
preferably, the surfactant is one or more of sodium cocoyl glutamate, sodium lauroyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, lauryl glucoside, coco glucoside, fatty alcohol polyoxyethylene ether sulfate and alpha-olefin sulfonate;
preferably, the surfactant is one or more of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, coco glucoside and alpha-alkenyl sulfonate;
further preferably, the surfactant is a mixture of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, coco glucoside and alpha-olefin sulfonate;
the mass ratio of the sodium cocoyl glutamate to the sodium cocoamidopropyl betaine to the sodium lauroamphoacetate to the cocoyl glucoside to the alpha-alkenyl sulfonate is 1-3:1-2:1:2-3: 1;
preferably, the mass ratio of the sodium cocoyl glutamate to the sodium cocoamidopropyl betaine to the sodium lauroamphoacetate to the cocoyl glucoside to the alpha-olefin sulfonate is 3:2:1:2: 1;
the invention can effectively improve the synergistic effect among the components by mixing various surfactants, so that the prepared shampoo product has better cleaning effect and can ensure the stability of foam.
Sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 1-3:1-2:1:2-3:1 are used as surfactants to be applied to a shampoo product and mixed with rice washing water fermentation liquor, can assist the components in the fermentation liquor to be absorbed by the hair, most of the shampoo products disclosed so far contain a plurality of components, but the hair washing effect is uneven, the main reason is that the effective components in the hair washing product can not be well absorbed by the hair, the invention can ensure that the effective components such as a plurality of proteins, amino acids, short peptides and the like contained in the rice washing water fermentation product are quickly and well absorbed by the hair by reasonably controlling the type and the mass ratio of the surfactant, thereby ensuring that the hair is smooth and bright.
The plant fermentation product is one or more of pomelo peel extract, polygonum multiflorum extract, mint extract, ginger extract, orange peel extract and pomegranate seed extract.
Preferably, the plant fermentation product is one or more of pomelo peel extract, mint extract, orange peel extract and pomegranate seed extract.
More preferably, the plant extract is one or more of pomelo peel extract, mint extract, orange peel extract and pomegranate seed extract.
Further preferably, the plant extracts are a shaddock peel extract, an orange peel extract and a pomegranate seed extract in a mass ratio of 2-5:1: 1-2.
Further preferably, the plant extracts are a shaddock peel extract, a tangerine peel extract and a pomegranate seed extract in a mass ratio of 5:1: 2.
According to the invention, the natural component plant extract is added into the shampoo product, and the pomelo peel extract, the orange peel extract and the pomegranate seed extract are selected to be used, so that the itching can be effectively relieved, and the bactericidal effect can be achieved.
The auxiliary agent is vitamin E and allantoin in a mass ratio of 2-5: 1.
Preferably, the auxiliary agent is vitamin E and allantoin in a mass ratio of 3-4: 1.
Still preferably, the auxiliary agent is vitamin E and allantoin in a mass ratio of 3: 1.
According to the invention, the vitamin E and the allantoin are added into the shampoo product in a mass ratio of 2-5:1, so that the nourishing effect of the shampoo product can be effectively improved, and the shampoo product can be mixed with other components to play a certain oil control effect.
The essence is conventional essence for shampoo products.
The invention also provides a preparation method of the shampoo product, which comprises the following steps:
premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 70-85 deg.C, and maintaining for 10-15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a microbial composition, which is applied to the fermentation of rice washing water by controlling the type and the mass ratio of microbes, can effectively improve the fermentation effect of the rice washing water, can obviously improve the content of various proteins, amino acids, short peptides and the like in a fermentation product, and fermentation liquor obtained by separating the fermentation product is used for mixing a shampoo product with other components, can improve the oil control and itching relieving performance of the shampoo product, and enables hair to be smooth and bright;
(2) the invention provides a shampoo product, which can effectively improve the synergistic effect among components by using a plurality of mixed surfactants, so that the prepared shampoo product has better cleaning effect and can ensure the stability of foam. The cocoyl sodium glutamate, the cocoamidopropyl betaine, the sodium lauroamphoacetate, the cocoyl glucoside and the alpha-olefin sulfonate which are used as the surfactants in the mass ratio of 1-3:1-2:1:2-3:1 are applied to shampoo products and are mixed with rice washing water fermentation products, so that the rice washing water fermentation products containing active ingredients such as various proteins, amino acids, short peptides and the like can be quickly and well absorbed by hair, and the hair is smooth and bright;
(3) according to the invention, the plant extracts are a shaddock peel extract, an orange peel extract and a pomegranate seed extract in a mass ratio of 2-5:1: 1-2; according to the invention, the natural component plant fermentation product is added into the shampoo product, and the pomelo peel extract, the orange peel extract and the pomegranate seed extract are selected to be used, so that the itching can be effectively relieved, and the sterilization effect can be achieved.
(4) According to the invention, the vitamin E and the allantoin are added into the shampoo product in a mass ratio of 2-5:1, so that the nourishing effect of the shampoo product can be effectively improved, and the shampoo product can be mixed with other components to play a certain oil control effect.
Deposit description
And (4) storage address: west road No. 1, north american society of science, china, and the date of collection: 21/09/2020
The strain name is as follows: pediococcus pentosaceus
Latin name: pediococcus pendosaceus
According to the biological materials (strains): PP-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20682
And (4) storage address: west road No. 1, north american society of science, china, and the date of collection: 21/09/2020
The strain name is as follows: bifidobacterium longum
Latin name: bifidobacterium longum
According to the biological materials (strains): BL-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20684
And (4) storage address: xilu No. 1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation date is as follows: 21/09/2020
The strain name is as follows: lactobacillus acidophilus
Latin name: lactobacillus acidophilus
According to the biological materials (strains): LA-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20683
Detailed Description
The present invention will be further illustrated in detail with reference to the following specific examples, which are not intended to limit the present invention but are merely illustrative thereof. The experimental methods used in the following examples are not specifically described, and the materials, reagents and the like used in the following examples are generally commercially available under the usual conditions without specific descriptions.
Basic embodiment a method for preparing rice washing water fermentation product
The method comprises the following steps:
soaking rice in distilled water, stirring, and filtering to obtain filtrate; putting the filtrate into a closed container, adding the microbial composition, vacuumizing, fermenting, and fermenting to obtain the rice washing water fermentation product.
Centrifuging the obtained rice washing water fermentation product, separating precipitate, repeatedly freezing and thawing the precipitate, centrifuging, drying and pulverizing to obtain filtrate, i.e. rice washing water fermentation lysate.
Filtering the obtained rice washing water fermentation product to obtain filtrate, namely the rice washing water extracellular substances.
Wherein, the mass percent of the rice is 0.5-2%; preferably 1-2%; and more preferably 1%; the stirring speed is 150-250 r/min; preferably 200 r/min; the fermentation temperature is 35-38 ℃; preferably 37 ℃; the fermentation time is 50-70 hours; preferably 60 hours.
The operation steps of the repeated freeze-thaw treatment are as follows: freezing the precipitate at-15 deg.C to-20 deg.C, thawing at room temperature, freezing at-15 deg.C to-20 deg.C, thawing at room temperature, and repeating for 2-3 times;
wherein the mass volume ratio of the microbial composition to the filtrate is 1-5%; preferably 3%.
The weight of thalli in the microbial composition is 0.2-0.4% of the weight of a microbial solution; preferably 0.3%.
The microbial composition is one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus;
the microorganism composition is pediococcus pentosaceus;
the microbial composition comprises pediococcus pentosaceus and bifidobacterium longum in a mass ratio of 1-2: 1;
the microbial composition is pediococcus pentosaceus and lactobacillus acidophilus in a mass ratio of 1: 1-2.5;
preferably, the microbial composition is a turbid mixture of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus.
Preferably, the ratio of bifidobacterium longum to lactobacillus acidophilus to pediococcus pentosaceus in the microbial composition is 1:2-5: 1-2; preferably 1:3 to 5: 2; still more preferably 1:5: 2.
The Pediococcus pentosaceus (Pediococcus pentosaceus) is Pediococcus pentosaceus PP-2020, has a preservation number of CGMCC No.20682, has a preservation time of 09 months and 21 days in 2020, is preserved in China general microbiological culture Collection center (CGMCC), and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus acidophilus LA-2020 with the preservation number of CGMCC No.20683 and the preservation time of 2020, 09 and 21 days, is preserved in China general microbiological culture Collection center (CGMCC) of China Committee for culture Collection of microorganisms, and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Bifidobacterium longum (Bifidobacterium longum) is Bifidobacterium longum BL-2020, the preservation number is CGMCC No.20684, the preservation time is 09/21 days in 2020, and the Bifidobacterium longum is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the Bifidobacterium longum are as follows: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
Example 1A Rice-washing Water fermentation product
In the basic embodiment, the microorganism is pediococcus pentosaceus;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 2A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is pediococcus pentosaceus and bifidobacterium longum in a mass ratio of 1: 1;
the mass volume ratio of the microbial composition to the filtrate is 5% (g/mL)
The weight of the cells in the microbial composition was 0.4% of the weight of the microbial solution.
Example 3A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is 2:1 pediococcus pentosaceus and bifidobacterium longum;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 4A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is pediococcus pentosaceus and lactobacillus acidophilus in a mass ratio of 1: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 4% (g/mL);
the weight of the cells in the microbial composition was 0.2% of the weight of the microbial solution.
Example 5A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is pediococcus pentosaceus and lactobacillus acidophilus in a mass ratio of 1: 2.5;
the mass-to-volume ratio of the microbial composition to the filtrate is 2% (g/mL);
the weight of the cells in the microbial composition was 0.4% of the weight of the microbial solution.
Example 6A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 1:2: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 7A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 1:3: 2;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 8A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 1:5: 2;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Application example 1A shampoo product
Comprises the following components in parts by weight: 15 parts of rice-washing water fermentation product prepared in example 1, 10 parts of sodium cocoyl glutamate, 5 parts of sodium lauroamphoacetate, 10 parts of decyl glucoside, 10 parts of shaddock peel extract, 10 parts of mint extract, 5 parts of vitamin E, 2 parts of essence and 15 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 70 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 2 shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 1, 15 parts of cocoyl potassium glycinate, 10 parts of sodium lauroyl glutamate, 15 parts of fatty alcohol-polyoxyethylene ether sulfate, 20 parts of pomelo peel extract, 10 parts of polygonum multiflorum extract, 10 parts of allantoin, 5 parts of essence and 30 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 10 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 3A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 1, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 4A shampoo product
The difference from application example 3 is that: the mass ratio of the cocoyl sodium glutamate to the cocoamidopropyl betaine to the sodium lauroamphoacetate to the cocoyl glucoside to the alpha-alkenyl sulfonate is 1:1:1:2:1, and the mass ratio of the pomelo peel extract to the orange peel extract to the red pomegranate seed extract is 2:1: 1; the mass ratio of the vitamin E to the allantoin is 2: 1; other operations and steps were the same as in application example 3.
Application example 5A shampoo product
The difference from application example 3 is that: the mass ratio of the cocoyl sodium glutamate to the cocoamidopropyl betaine to the sodium lauroamphoacetate to the cocoyl glucoside to the alpha-olefin sulfonate is 3:2:1:3: 1; the mass ratio of the pomelo peel extract to the orange peel extract to the pomegranate seed extract is 5:1: 1; the mass ratio of the vitamin E to the allantoin is 5: 1; other operations and steps were the same as in application example 3.
Application example 6 shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 2, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 7A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 3, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 8A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 4, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 9A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 5, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 10A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 6, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 11A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 7, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 12A shampoo product
Comprises the following components in parts by weight: 25 parts of rice washing water fermentation product prepared in example 8, 36 parts of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-olefin sulfonate in a mass ratio of 3:2:1:2:1, 32 parts of shaddock peel extract, orange peel extract and red pomegranate seed extract in a mass ratio of 5:1:2, 8 parts of vitamin E and allantoin in a mass ratio of 3:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract and adjuvant into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 13A shampoo product
The difference from application example 12 is that: the rice-washing water fermentation lysate prepared in example 8 was used in the composition; the other steps and operations were the same as those in application example 12.
Application example 14A shampoo product
The difference from application example 12 is that: the rice-washing water fermentation broth prepared in example 8 was used as the component; the other steps and operations were the same as those in application example 12.
Comparative example 1
The rice-washing water fermentation was performed using the method disclosed in patent application CN110200889A to obtain a fermentation product for use in the formulation of example 4 to obtain a shampoo product.
Comparative example 2
The difference from example 4 is that: the ratio of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in the microbial composition for fermentation is 1:8: 1; the other operations and steps are the same as in example 4.
Comparative example 3
The difference from example 4 is that: the ratio of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in the microbial composition for fermentation is 1:2: 5; the other operations and steps are the same as in example 4.
Comparative example 4
The difference from example 4 is that: the mass ratio of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoglucoside and alpha-olefin sulfonate was 5:1:1:4:1, and the other operations and steps were the same as in example 4.
Comparative example 5
The difference from example 4 is that: the mass ratio of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoglucoside and alpha-olefin sulfonate was 1:3:1:1:3, and the other operations and steps were the same as in example 4.
Comparative example 6
The difference from example 4 is that: the mass ratio of the pomelo peel extract, the orange peel extract and the pomegranate seed extract was 6:1:3, and the other operations and steps were the same as in example 4.
Comparative example 7
The difference from example 4 is that: the mass ratio of the pomelo peel extract to the orange peel extract was 5:1, and the other operations were the same as in example 4.
Comparative example 8
The difference from example 4 is that: the procedure was the same as in example 4 except that no auxiliary was added.
Comparative example 9
The difference from example 4 is that: the mass ratio of vitamin E to allantoin was 8:1, and the other operations were the same as in example 4.
Effect test
Test 1, evaluation of oil control Effect
1. Principle of experiment
The skin was tested for oil content using the oil tester SebumeterSM 815. Based on the principle of a photometer, a special extinction adhesive tape with the thickness of 0.1mm can be changed into a semitransparent adhesive tape after absorbing grease on human skin, the light transmission quantity of the semitransparent adhesive tape can be changed, the more grease is absorbed, the greater the light transmission quantity is, and the content of the grease on the skin can be measured.
2. Test method
At a shampoo shop in a certain market, 210 oily scalp subjects were randomly searched, 10 per group. The subjects were rested for 30 minutes in a laboratory at a temperature of 22. + -. 3 ℃ and a relative humidity of 50. + -.5%, and skin oil content of the left and right forehead was measured using an oil tester SebumetersM815, as data D0, and samples of the shampoo products prepared using examples 1-14 and comparative examples 1-5, 8-9 were distributed to each subject, shampooed once a day for 28 days, and skin oil content was measured in a thermostated laboratory on days 14 and 28, respectively, as data D14 and D28. Respectively calculating the oil content change rate of D14 and D28 according to the following formula:
the change rate of the oil content { (oil content after use of sample-oil content before use of sample)/oil content before use of sample } × 100%, and the detection results are shown in tables 1 to 2 below.
TABLE 1 rate of change of oil and fat content of shampoo products prepared using examples 1-14
| Change rate of oil content in 14 days% | Change rate of oil content in 28 days% | |
| Application example 1 | -18.2 | -23.7 |
| Application example 2 | -18.5 | -24.5 |
| Application example 3 | -23.0 | -28.2 |
| Application example 4 | -21.8 | -26.9 |
| Application example 5 | -20.7 | -25.1 |
| Application example 6 | -24.8 | -28.3 |
| Application example 7 | -24.4 | -30.2 |
| Application example 8 | -25.2 | -29.9 |
| Application example 9 | -24.6 | -30.9 |
| Application example 10 | -29.8 | -36.7 |
| Application example 11 | -30.2 | -37.1 |
| Application example 12 | -35.8 | -42.6 |
| Application example 13 | -27.4 | -32.3 |
| Application example 14 | -28.1 | -33.4 |
According to the detection data in the table 1, the shampoo products prepared in the application examples 10-12 of the present invention have a good oil control effect, and especially the shampoo product prepared in the application example 12 has the best oil control effect, wherein the oil content change rate is-35.8% in 14 days and-42.6% in 28 days.
TABLE 2 Rate of change of oil and fat content of shampoo products prepared in comparative examples 1-5 and 8-9
| Change rate of oil content in 14 days% | Change rate of oil content in 28 days% | |
| Comparative example 1 | -18.3 | -23.5 |
| Comparative example 2 | -24.8 | -29.6 |
| Comparative example 3 | -23.6 | -29.1 |
| Comparative example 4 | -25.0 | -31.8 |
| Comparative example 5 | -26.4 | -30.5 |
| Comparative example 8 | -26.1 | -31.4 |
| Comparative example 9 | -25.4 | -30.7 |
According to the detection data in the table 2, it can be seen that the comparative examples 1-3 change the type of the fermenting microorganism to have the greatest influence on the oil control effect of the shampoo product; comparative examples 4 to 5, when the mass ratio of the components of the surfactant was changed out of the range of the present invention, the oil control effect of the shampoo product was reduced, but it was still better than that of application examples 1 to 9, and it was thus understood that the kind and mass ratio of the surfactant had an influence on the performance of the shampoo product. Similarly, in comparative examples 8 to 9 in which no adjuvant was added or the mass ratio of the adjuvant was changed out of the range claimed in the present invention, the oil-controlling property of the resulting shampoo product was significantly lowered as compared with that of application example 12, and thus it was found that the oil-controlling effect of the shampoo product could be improved by the interaction of the main adjuvant and the surfactant.
Test 2 evaluation of antipruritic efficacy
160 volunteers with scalp itching and other symptoms between 18-50 years of age were randomly searched, wherein 80 men and women, divided into 16 groups of 10 persons, were used once a day for one month continuously using the shampoo product samples prepared in application examples 1-14 and comparative examples 6-7, respectively, to observe and feel the use effect.
The testers score the improvement condition of the pruritus, the full score is 10 parts, the average score is taken, the higher the score is, the better the improvement effect is, and the specific scoring condition is shown in tables 3-4 below.
TABLE 3 Scoring of shampoo products prepared using examples 1-14
According to the score data of table 3 above, it can be seen that the shampoo products prepared in examples 10-12 of the present invention have very good antipruritic effect, and especially the shampoo product prepared in example 4 has the highest score of 9.5 parts and the best antipruritic effect, so that the shampoo products prepared in the present invention have very good antipruritic effect.
TABLE 4 Scoring of shampoo products prepared in comparative examples 6-7
| Scoring | |
| Comparative example 6 | 8.2 |
| Comparative example 7 | 7.5 |
From the test data of Table 4 above, it can be seen that the antipruritic effect of the shampoo products is reduced in the comparative examples 6 to 7 in which the ratio of the components of the plant extracts is changed or one of the plant extracts is omitted, but is still superior to that of examples 1 to 3, and thus it is known that the components of the plant extracts and the ratio of the components have an influence on the performance of the shampoo products.
Test example 3 Hair texture pliability test
Female volunteers with long hair between 18-50 years of age were randomly searched for 50 persons, divided into 10 groups of 5 persons, and the shampoo product samples prepared in application examples 10-12 and comparative examples 1-3 were used once a day for one month continuously, and the use effect was observed and felt.
The tester scores the improvement condition of the hair smoothness, the hair smoothness is fully divided into 5 parts, the average is taken out, the higher the score is, the better the improvement effect is, and the specific scoring condition is shown in the following tables 5-6.
TABLE 5
| Scoring | |
| Application example 10 | 4.2 |
| Application example 11 | 4.1 |
| Application example 12 | 4.6 |
According to the score data in table 5 above, the hair softening degree of the shampoo products prepared in the application examples 10 to 12 of the present invention is improved after use, and particularly, the hair softening degree of the shampoo products prepared in the application example 12 is the best 4.6 parts at the maximum, so that the shampoo products prepared in the present invention have the effect of softening the hair.
TABLE 6 evaluation of shampoo products prepared in comparative examples 1 to 3
| Scoring | |
| Comparative example 1 | 3.0 |
| Comparative example 2 | 3.5 |
| Comparative example 3 | 3.6 |
According to the detection data in table 6, it can be seen that the hair washing product prepared by the fermentation liquid obtained by fermenting the rice washing water by using the traditional method in the comparative example 1 has poor improvement effect on the softness of the hair, and the score is obviously inferior to that of the hair washing product prepared by the embodiment of the invention; comparative example 2 and comparative example 3 when the mass ratio of the microorganisms changed during the fermentation process was not within the range claimed in the present invention, the hair care product obtained also exhibited a certain reduction in the property of improving the softness of the hair, indicating that the mass ratio of the microorganisms significantly affected the influencing ingredients in the fermentation broth, thereby deteriorating the property of the hair care product.
In conclusion, the shampoo product obtained by mixing the rice washing water fermentation liquid obtained by using the microorganisms with the mass ratio disclosed by the invention for rice washing water fermentation and the surfactant, the plant extract and the auxiliary disclosed by the invention has the double effects of controlling oil, relieving itching and enabling the hair to be smooth and glossy.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (3)
1. A shampoo product, which is characterized in that: comprises 15-25 parts of rice washing water fermentation product of microbial composition, 25-40 parts of surfactant, 20-30 parts of plant extract, 5-10 parts of auxiliary agent, 2-5 parts of essence and 15-30 parts of deionized water;
the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a ratio of 1:2-5: 1-2; the pediococcus pentosaceus（Pediococcuspentosaceus）Is pediococcus pentosaceus PP-2020 with the preservation number of CGMCC No.20682, the preservation time of 21 days 09 months in 2020, and the preservation time is CGMCC which is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms and has the address as follows: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the Bifidobacterium longum（Bifidobacterium longum）The preservation number of the strain is CGMCC No.20684, the preservation time is 09 and 21 days in 2020, and the strain is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the strain are as follows: microorganism of western road No. 1 Hospital No. 3, China academy of sciences, Beijing, Chaoyang, regionA research institute; the lactobacillus acidophilus（Lactobacillus acidophilus）The preservation number of the strain is CGMCC No.20683, the preservation time is 09 and 21 days in 2020, and the strain is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the strain are as follows: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences;
the surfactant is a mixture of sodium cocoyl glutamate, cocamidopropyl betaine, sodium lauroamphoacetate, cocoyl glucoside and alpha-alkenyl sulfonate in a mass ratio of 1-3:1-2:1:2-3: 1;
the auxiliary agent is vitamin E and allantoin in a mass ratio of 2-5: 1;
the plant extracts comprise a shaddock peel extract, an orange peel extract and a pomegranate seed extract in a mass ratio of 2-5:1: 1-2.
2. The shampoo product of claim 1, wherein the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a ratio of 1:5: 2.
3. The shampoo product of claim 1 wherein the rice-washing water fermentation product is a fermentation lysate or/and fermentation exosomes.
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