CN112442466B - Rice washing water fermentation product with scalp aging resisting function and preparation method thereof - Google Patents
Rice washing water fermentation product with scalp aging resisting function and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a rice washing water fermentation product with a scalp aging resisting function and a preparation method thereof, and belongs to the technical field of fermentation. On one hand, the invention provides a microbial composition for rice washing water fermentation, wherein the microbial composition contains one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus, and provides application of the microbial composition in preparation of rice washing water fermentation products.
Description
Technical Field
The invention belongs to the technical field of fermentation, and particularly relates to a rice washing water fermented product with a scalp aging resisting function and a preparation method thereof.
Background
The rice is one of the main grain crops in China and the second crop in the world, and the annual output of China exceeds 2.0 hundred million tons. The rice washing water is water left after washing rice, and mainly contains nutritional ingredients of rice bran and aleurone layer, including protein, dietary fiber, starch, oryzanol, vitamins, minerals and other bioactive ingredients. The rice bran protein has anticancer activity and health promotion function, and the rice bran peptide is rice bran proteinThe low molecular weight polypeptide obtained by enzymatic hydrolysis has the characteristics of low allergy and easy dissolution. There are researchers subjecting rice bran to CO2The extract shows good hair growth effect by supercritical extraction, and the hair follicles are increased remarkably. The rice washing water is put into a basin or a barrel and the like for natural fermentation every day by the people of the Dai nationality in Yunnan, and the traditional fermented rice washing water is used for washing hair and has the effects of removing dandruff, moistening hair, brightening hair, promoting hair to turn black and thick and the like.
Therefore, there have been increasing studies on the use of fermented rice washing water as a cleansing product, and as disclosed in, for example, chinese patent application 201610882067.1, a functional cosmetic composition for anti-oxidation, anti-wrinkle and moisturizing improvement containing a fermented rice washing water culture as an active ingredient and a method for preparing the same, wherein the cosmetic composition has an excellent effect of providing a functional cosmetic composition containing a fermented rice washing water culture as an active ingredient by comparing and confirming the anti-oxidation, anti-wrinkle and moisturizing improvement effects before and after fermentation of the rice washing water as a cosmetic raw material, but the anti-scalp aging performance of the fermented rice washing water is not studied.
For another example, Chinese patent application 201810502292.7 discloses a rice washing shampoo product, which adopts a pure natural formula and a modern biological fermentation synthesis technology to innovatively improve the rice washing shampoo formula, on the basis of obviously increasing active substances through biological fermentation of rice washing water, the biosurfactant compound liquid is added, and linoleic acid, bamboo leaf flavonoid, chitosan and the like are further introduced for compounding, the components have synergistic interaction, the scalp and hair can be gently cleaned, unexpected technical effects are generated in the field of hair loss prevention and/or hair growth and the aspects of relieving itching and removing dandruff, in addition, the high-efficiency absorption of active ingredients can be promoted, therefore, hair nourishing, hair care, hair fixing and hair growth are realized, and the application is suitable for long-term use, but the application uses the traditional saccharomycetes for fermentation in the fermentation process, and the anti-aging performance of the scalp is not researched.
However, according to the description of the prior art, the conventional strains are used for fermentation when rice washing water is fermented, and although the conventional microorganisms have certain effects in the rice washing water fermentation process, in view of the poor rice washing water fermentation effect of the conventional microorganisms and the single type of microorganisms in the obtained fermented product, it is necessary to screen and optimize the combined microorganisms to enhance the fermentation effect of the microorganisms, and in view of the synergistic effect of the multiple microorganisms which are prepared in a compounding manner and can synergistically enhance the functions of each other or improve the synergistic capability of the microorganisms, so that the preparation has more functions and better effects, and 3 strains with good effects, namely Bifidobacterium longum (Bifidobacterium longum), Lactobacillus acidophilus (Lactobacillus acidophilus) and Pediococcus pentosaceus (Pediococcus pentosaceus), are finally obtained through selection. The results show that the rice washing water is fermented by using the above-mentioned bacteria mixture, and the fermented product is separated, so that the obtained rice washing water fermented lysate can be used in shampoo product, and can effectively raise the scalp ageing-resisting property of shampoo product.
The applicant group utilizes the optional combination of 3 screened beneficial bacteria (bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus) to prepare a microorganism compound preparation, performs fermentation treatment on rice washing water, combines with the specific combination proportion of plant extracts and shampoo product auxiliary materials, observes the fermentation capacity of the microorganism compound preparation rice washing water on the one hand, uses the obtained fermentation product in shampoo on the one hand, performs product development, respectively obtains shampoo products with different efficacies of various specific compound combination proportions and plant extracts and auxiliary material combination proportions, and performs patent protection respectively.
Disclosure of Invention
The applicant group of the invention respectively identifies 3 strains of microorganisms, namely lactobacillus acidophilus, bifidobacterium longum and pediococcus pentosaceus, by screening and purifying natural fermentation liquor or purely culturing the 3 strains of microorganisms, and preserves the 3 strains of microorganisms, wherein the preservation information is as follows:
the Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus acidophilus LA-2020 with the preservation number of CGMCC No.20683 and the preservation time of 2020, 09 and 21 days, is preserved in China general microbiological culture Collection center (CGMCC) of China Committee for culture Collection of microorganisms, and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Bifidobacterium longum (Bifidobacterium longum) is Bifidobacterium longum BL-2020, the preservation number is CGMCC No.20684, the preservation time is 09/21 days in 2020, and the Bifidobacterium longum is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the Bifidobacterium longum are as follows: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Pediococcus pentosaceus (Pediococcus pentosaceus) is Pediococcus pentosaceus PP-2020, has a preservation number of CGMCC No.20682, has a preservation time of 09 months and 21 days in 2020, is preserved in China general microbiological culture Collection center (CGMCC), and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The technical development is carried out on the compound fermentation product of the 3 strains of microorganisms to obtain a fermentation product.
The preparation method of the fermentation product comprises the step of fermenting the mixed culture raw material by using the microbial composition, wherein the mixed culture raw material at least comprises rice washing water.
More specifically, the preparation method of the fermentation product comprises the following steps:
soaking rice in distilled water, stirring, and filtering to obtain filtrate; placing the filtrate in a closed container, adding the above microorganism composition, vacuumizing, fermenting, and fermenting to obtain rice washing water fermentation product;
centrifuging the obtained rice washing water fermentation product, separating precipitate, repeatedly freezing and thawing the precipitate, centrifuging, drying and pulverizing to obtain filtrate, i.e. rice washing water fermentation lysate.
Filtering the obtained rice washing water fermentation product to obtain filtrate, namely the rice washing water extracellular substances.
Wherein, the mass percent of the rice is 0.5-2%; preferably 1-2%; and more preferably 1%; the stirring speed is 150-250 r/min; preferably 200 r/min; the fermentation temperature is 35-38 ℃; preferably 37 ℃; the fermentation time is 50-70 hours; preferably 60 hours.
The operation steps of the repeated freeze-thaw treatment are as follows: freezing the precipitate at-15 deg.C to-20 deg.C, thawing at room temperature, freezing at-15 deg.C to-20 deg.C, thawing at room temperature, and repeating for 2-3 times;
wherein the mass volume ratio of the microbial composition to the filtrate is 1-5%; preferably 3%.
The weight of thalli in the microbial composition is 0.2-0.4% of the weight of a microbial solution; preferably 0.3%.
The rice is one or more of rice, millet and glutinous rice.
According to the invention, the rice washing water is fermented by using the microbial composition, so that the contents of protein, amino acid, short peptide and the like in a rice washing water fermentation product are greatly improved.
The microbial composition is one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus;
the microbial composition is lactobacillus acidophilus;
the microbial composition is bifidobacterium longum and lactobacillus acidophilus in a mass ratio of 1-3: 1;
the microbial composition is lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 2-5: 1;
preferably, the microbial composition is a turbid mixture of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus.
Preferably, the ratio of bifidobacterium longum to lactobacillus acidophilus to pediococcus pentosaceus in the microbial composition is 3-5:2-3: 1; preferably 4-5:3: 1; still more preferably 5:3: 1.
In the implementation process, the invention unexpectedly discovers that the proportion of three microorganisms, namely bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus, has obvious influence on the components in the fermentation product.
The microbial fermentation efficiency can be effectively improved by controlling the ratio of bifidobacterium longum to lactobacillus acidophilus to pediococcus pentosaceus to be 3-5:2-3:1, and the rice washing water fermented product obtained by fermenting the rice washing water by using the strains in the ratio has more components and good quality.
According to the invention, the rice washing water is fermented by the obtained microbial composition through reasonably controlling the mass ratio of the microbes, and the fermentation product contains various microbial components, so that the scalp aging resistance of the shampoo product can be obviously improved when the microbial composition is applied to the shampoo product.
The invention also provides a shampoo product, which contains the rice washing water fermentation product prepared by the method.
The fermentation product is a fermentation lysate or/and a fermentation extracellular substance.
The shampoo product has the function of resisting scalp aging.
The shampoo product comprises the following components in parts by weight: 5-10 parts of rice washing water fermentation product, 10-30 parts of surfactant, 30-50 parts of plant extract, 2-5 parts of essence and 10-20 parts of deionized water.
Preferably, the shampoo product comprises the following components in parts by weight: 6-8 parts of rice washing water fermentation product, 15-25 parts of surfactant, 40-50 parts of plant extract, 3-5 parts of essence and 15-18 parts of deionized water.
Still preferably, the shampoo product comprises the following components in parts by weight: 8 parts of rice washing water fermentation product, 20 parts of surfactant, 48 parts of plant extract, 3 parts of essence and 18 parts of deionized water.
The surfactant is selected from one or more of N-acyl sodium glutamate, dodecyl dimethyl betaine, amidopropyl amine oxide and coconut oil monoethanolamide sulfo succinic acid monoester disodium salt;
preferably, the surfactant is selected from one or more of N-acyl sodium glutamate, dodecyl dimethyl betaine and amidopropyl amine oxide;
still more preferably, the surfactant is selected from the group consisting of a mixture of sodium N-acyl glutamate and amidopropyl amine oxide;
the surfactant is a mixture of N-acyl sodium glutamate and amidopropyl amine oxide in a mass ratio of 1-3:1, and preferably 2: 1.
The surfactant provided by the invention can improve the absorption of the hair on effective components, and improve the scalp aging resistance of the shampoo product on the basis of improving the cleaning effect of the shampoo product.
The plant extract is selected from one or more of purslane extract, pseudo-ginseng extract, catechu extract and kelp extract.
Preferably, the plant extract is selected from one or more of pseudo-ginseng extract, catechu extract and kelp extract.
More preferably, the plant extract is selected from one or more of catechu extract and kelp extract.
The mass ratio of the catechu extract to the kelp extract is 1: 1.
The mass ratio of the rice washing water fermentation product to the plant extract is 1: 3-10; preferably 1: 5-8; still more preferably 1: 6.
In the implementation process, the invention unexpectedly discovers that the shampoo product obtained by controlling the mass ratio of the rice washing water fermentation product to the plant extract to be 1:3-10 has the effects of improving hair follicles, enhancing the vitality of hair cells, fully supplementing nutrition, reducing hair damage, moistening hair and endowing hair with luster.
The essence is conventional essence for shampoo products.
As a preferred embodiment, the invention provides a shampoo product, which comprises 5-10 parts of rice washing water fermentation product, 10-30 parts of dodecyl dimethyl betaine, 30-50 parts of purslane extract, 2-5 parts of essence and 10-20 parts of deionized water.
As another preferred embodiment, the present invention provides a shampoo product comprising, in parts by weight: 5-10 parts of rice washing water fermentation product, 10-30 parts of N-acyl sodium glutamate, 30-50 parts of catechu extract, 2-5 parts of essence and 10-20 parts of deionized water.
As another preferred embodiment, the present invention provides a shampoo product comprising, in parts by weight: 5-10 parts of rice washing water fermentation product, 10-30 parts of coconut monoethanolamide sulfosuccinic acid monoester disodium salt, 30-50 parts of pseudo-ginseng extract, 2-5 parts of essence and 10-20 parts of deionized water.
As another more preferred embodiment, the present invention provides a shampoo product comprising, in parts by weight: 8 parts of rice washing water fermentation product, 20 parts of mixture of N-acyl sodium glutamate and amidopropyl amine oxide in a mass ratio of 1-3:1, 48 parts of a mixture of catechu extract and kelp extract, 3 parts of essence and 18 parts of deionized water.
The invention also provides a preparation method of the shampoo product, which comprises the following steps:
premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 70-85 deg.C, and maintaining for 10-15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a microbial composition, which is applied to the fermentation of rice washing water by controlling the types and the mass ratio of microbes, can effectively improve the fermentation effect of the rice washing water, improve the activity of substances in cells, and can effectively adjust the performance of resisting scalp aging when the obtained fermentation product is used for preparing a shampoo product;
(2) the invention provides a shampoo product, which contains rice washing water fermentation product, and can improve scalp hair follicles, enhance the activity of hair cells, fully supplement nutrition, reduce hair damage, moisten hair and endow hair with luster by controlling the mass ratio of the rice washing water fermentation product to the plant extract to be 1: 3-10;
(3) according to the invention, the mixture of N-acyl sodium glutamate and amidopropyl amine oxide in a mass ratio of 1-3:1 is added into the shampoo product as a surfactant, so that the absorption of hair on effective components can be improved, the scalp aging resistance function of the shampoo product is improved on the basis of improving the cleaning effect of the shampoo product, the alopecia can be effectively improved, and the scalp water content is increased.
Deposit description
And (4) storage address: xilu No. 1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation date is as follows: 21/09/2020
The strain name is as follows: bifidobacterium longum
Latin name: bifidobacterium longum
According to the biological materials (strains): BL-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20684
And (4) storage address: xilu No. 1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation date is as follows: 21/09/2020
The strain name is as follows: lactobacillus acidophilus
Latin name: lactobacillus acidophilus
According to the biological materials (strains): LA-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20683
And (4) storage address: xilu No. 1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation date is as follows: 21/09/2020
The strain name is as follows: pediococcus pentosaceus
Latin name: pediococcus pendosaceus
According to the biological materials (strains): PP-2020
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Registration number of the preservation center: CGMCC No.20682
Detailed Description
The present invention will be further illustrated in detail with reference to the following specific examples, which are not intended to limit the present invention but are merely illustrative thereof. The experimental methods used in the following examples are not specifically described, and the materials, reagents and the like used in the following examples are generally commercially available under the usual conditions without specific descriptions.
Basic embodiment a method for preparing a rice washing water fermentation product
The method comprises the following steps:
soaking rice in distilled water, stirring, and filtering to obtain filtrate; putting the filtrate into a closed container, adding the microbial composition, vacuumizing, fermenting, and fermenting to obtain the rice washing water fermentation product.
Centrifuging the obtained rice washing water fermentation product, separating precipitate, repeatedly freezing and thawing the precipitate, centrifuging, drying and pulverizing to obtain filtrate, i.e. rice washing water fermentation lysate.
Filtering the obtained rice washing water fermentation product to obtain filtrate, namely the rice washing water extracellular substances.
Wherein, the mass percent of the rice is 0.5-2%; preferably 1-2%; and more preferably 1%; the stirring speed is 150-250 r/min; preferably 200 r/min; the fermentation temperature is 35-38 ℃; preferably 37 ℃; the fermentation time is 50-70 hours; preferably 60 hours.
The operation steps of the repeated freeze-thaw treatment are as follows: freezing the precipitate at-15 deg.C to-20 deg.C, thawing at room temperature, freezing at-15 deg.C to-20 deg.C, thawing at room temperature, and repeating for 2-3 times;
wherein the mass volume ratio of the microbial composition to the filtrate is 1-5%; preferably 3%.
The weight of thalli in the microbial composition is 0.2-0.4% of the weight of a microbial solution; preferably 0.3%.
The microbial composition is one or more of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus;
the microbial composition is lactobacillus acidophilus;
the microbial composition is bifidobacterium longum and lactobacillus acidophilus in a mass ratio of 1-3: 1;
the microbial composition is lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 2-5: 1;
preferably, the microbial composition is a turbid mixture of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus.
Preferably, the microbial composition is bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 3-5:2-3: 1; preferably 4-5:3: 1; still more preferably 5:3: 1.
The Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus acidophilus LA-2020 with the preservation number of CGMCC No.20683 and the preservation time of 2020, 09 and 21 days, is preserved in China general microbiological culture Collection center (CGMCC) of China Committee for culture Collection of microorganisms, and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Bifidobacterium longum (Bifidobacterium longum) is Bifidobacterium longum BL-2020, the preservation number is CGMCC No.20684, the preservation time is 09/21 days in 2020, and the Bifidobacterium longum is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the Bifidobacterium longum are as follows: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
The Pediococcus pentosaceus (Pediococcus pentosaceus) is Pediococcus pentosaceus PP-2020, has a preservation number of CGMCC No.20682, has a preservation time of 09 months and 21 days in 2020, is preserved in China general microbiological culture Collection center (CGMCC), and has the following addresses: xilu No. 1, Beijing, Chaoyang, Beijing, and institute for microbiology, China academy of sciences.
Example 1A Rice-washing Water fermentation product
In the basic embodiment, the microorganism is lactobacillus acidophilus;
the mass volume ratio of the microbial composition to the filtrate is 5% (g/mL)
The weight of the cells in the microbial composition was 0.4% of the weight of the microbial solution.
Example 2A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is bifidobacterium longum and lactobacillus acidophilus in a mass ratio of 1: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 3A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is bifidobacterium longum and lactobacillus acidophilus in a mass ratio of 3: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 4% (g/mL);
the weight of the cells in the microbial composition was 0.2% of the weight of the microbial solution.
Example 4A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 2: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 2% (g/mL);
the weight of the cells in the microbial composition was 0.4% of the weight of the microbial solution.
Example 5A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition is lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 5: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 5% (g/mL);
the weight of the cells in the microbial composition was 0.2% of the weight of the microbial solution.
Example 6A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 3:2: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Example 7A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 4:3: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 4% (g/mL);
the weight of the cells in the microbial composition was 0.4% of the weight of the microbial solution.
Example 8A Rice-washing Water fermentation product
In the basic embodiment, the microbial composition comprises the bifidobacterium longum, the lactobacillus acidophilus and the pediococcus pentosaceus in a mass ratio of 5:3: 1;
the mass-to-volume ratio of the microbial composition to the filtrate is 3% (g/mL);
the weight of the cells in the microbial composition was 0.3% of the weight of the microbial solution.
Application example 1A shampoo product
Comprises the following components in parts by weight: 5 parts of rice washing water fermentation product prepared in example 1, 10 parts of dodecyl dimethyl betaine, 40 parts of purslane extract, 2 parts of essence and 10 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 70 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 2 shampoo product
Comprises the following components in parts by weight: 10 parts of rice washing water fermentation product prepared in example 1, 30 parts of sodium N-acyl glutamate, 30 parts of catechu extract, 5 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 10 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 3A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 1, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 4A shampoo product
Comprises the following components in parts by weight: 10 parts of rice washing water fermentation product prepared in example 1, 28 parts of mixture of N-acyl sodium glutamate and amidopropyl amine oxide in a mass ratio of 3:1, 30 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 4 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 70 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 5A shampoo product
Comprises the following components in parts by weight: 6 parts of rice washing water fermentation product prepared in example 1, 20 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 1:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 20 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 70 deg.C, and maintaining for 10 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 6 shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 2, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 7A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 3, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 8A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 4, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 9A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 5, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 10A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 6, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 11A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 7, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 12A shampoo product
Comprises the following components in parts by weight: 8 parts of rice washing water fermentation product prepared in example 8, 21 parts of mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, 48 parts of mixture of catechu extract and kelp extract in a mass ratio of 1:1, 3 parts of essence and 18 parts of deionized water.
The preparation method comprises the following steps: premixing a surfactant, a rice washing water fermentation product and deionized water, and then adding the mixture into a stirring pot for homogenizing and dissolving to obtain a mixture 1; adding plant extract into mixture 1, stirring for homogenizing to dissolve, heating to 85 deg.C, and maintaining for 15 min to obtain mixture 2; and (3) cooling the mixture 2, adding essence when the temperature is cooled to 35 ℃, stirring to completely dissolve the essence, and cooling to room temperature to obtain the required product.
Application example 13A shampoo product
The difference from application example 12 is that: the rice-washing water fermentation lysate prepared in example 8 was used in the composition; the other steps and operations were the same as those in application example 12.
Application example 14A shampoo product
The difference from application example 12 is that: the rice-washing water fermentation broth prepared in example 8 was used as the component; the other steps and operations were the same as those in application example 12.
Comparative example 1
The difference from application example 12 is that: the ratio of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in the microbial composition for fermentation is 5:1: 1; the other operations and steps are the same as in example 4.
Comparative example 2
The difference from application example 12 is that: the ratio of bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in the microbial composition for fermentation is 1:1: 3; the other operations and steps are the same as in example 4.
Comparative example 3
The difference from application example 12 is that: the mass ratio of the mixture of sodium N-acylglutamate and aminopropyl amine oxide was 0.5:1, and the other operations were the same as in example 4.
Comparative example 4
The difference from application example 12 is that: the mass ratio of the mixture of sodium N-acylglutamate and aminopropyl amine oxide was 4:1, and the other operations were the same as in example 4.
Comparative example 5
The difference from application example 12 is that: the mass ratio of the rice washing water fermentation lysate to the plant extract was 0.5:1, and the other operations and steps were the same as in example 4.
Comparative example 6
The difference from application example 12 is that: the mass ratio of the rice washing water fermentation lysate to the plant extract was 2:1, and the other operations and steps were the same as in example 4.
Effect test
1. Hair density detection
200 consumers with alopecia symptoms were randomly searched in a shampoo shop of a certain market, and then a group of 10 consumers, each of which washed the hair for 2 to 3 days once using the shampoo products prepared in the application examples 1 to 14 of the present invention and the comparative examples 1 to 6 for a test period of 3 months, observed whether the alopecia was improved, and measured for hair density by a hair photography (photo trichgram).
The measurement method is carried out in a predetermined area (1 cm)2) And the average value was calculated using the number of hairs in the area as evaluation data, and the specific data is shown in table 1 below.
TABLE 1 Hair Density test results
As can be seen from the test data in table 1 above, the shampoo products prepared in application examples 1-5 have certain effects of anti-alopecia and promoting hair growth, and the shampoo products prepared in application example 3 using lactobacillus acidophilus as a microorganism for fermentation to obtain a rice-washing water fermentation product for preparing the shampoo product, and controlling the surfactant to be a mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, and the plant extract to be a mixture of catechu extract and kelp extract in a mass ratio of 1:1, and controlling the mass ratio of the rice-washing water fermentation product to the plant extract to be 1:6 are the best.
To further prove that the shampoo products prepared according to the present invention can prevent scalp aging, prevent hair loss and promote hair growth, the above experiments were performed on the shampoo products prepared in application examples 6 to 12, and the results are shown in table 2 below.
According to the detection results in the table 2, the types of the microbial compositions are changed, namely when the types of the microorganisms are increased to two, the obtained shampoo has good effects of resisting alopecia and promoting hair growth compared with the shampoo prepared by microbial fermentation; application examples 10 to 12 shampoo prepared by fermenting three kinds of microorganisms has better anti-hair loss and hair growth promoting effects, and changing the mass ratio of the microorganisms also significantly affects the performance of the shampoo, and according to the above table, it can be seen that the effect is best when the microbial composition is a fermentation liquid obtained when bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus are mixed in a mass ratio of 5:3:1, a mixture of sodium N-acyl glutamate and amidopropyl amine oxide is added in a mass ratio of 2:1, a mixture of catechu extract and kelp extract is mixed in a mass ratio of 1:1, and a mass ratio of rice-washing water fermented product to plant extract is controlled to be 1: 6.
To further demonstrate the above effects, the shampoo products prepared in comparative examples 1 to 6 were subjected to the above experiments, and the results are shown in Table 3 below.
TABLE 3 Hair Density test results
According to the detection data in the above table 3, it can be seen that when the mass ratio of the modified microorganism is not within the protection range of the present invention, the efficacy of the prepared shampoo product for resisting alopecia and promoting hair growth is significantly reduced, which is equivalent to the effect of the application example 3; the change of the mass ratio of the components in the surfactant can obviously influence the anti-alopecia and hair growth promoting effects of the shampoo product, and because the type of the surfactant can obviously influence the absorption of the hair on the shampoo product, the anti-alopecia and hair growth promoting effects can be obviously influenced; the change of the mass ratio of the rice washing water fermentation product to the plant extract can affect the overall quality of the product to a certain extent, thereby affecting the efficacy of resisting alopecia and promoting hair growth.
2. Hair follicle-improving effect test
(1) Experimental animals: hair belongs to resting Kunming rats.
(2) Mouse hair loss model: the rats were subjected to a depilation treatment, and the growth period of hair was confirmed to be in the telogen phase.
(3) Grouping and administration: after 24 hours after depilation, 25 rats with smooth and skin-free depilated areas were selected, randomly divided into 5 groups of 5, and washed with the shampoo products prepared in application examples 1-5, respectively, at an area of 2cm × 3cm, once for 2 days, for 10 days. The blank groups were washed with clear water, and on day 10, skin tissue blocks were taken from the same positions on the backs of the mice in each group, fixed with 10% formalin, paraffin-embedded, sliced to a thickness of about 4-5 μm, stained by HE, and observed under light. Hair follicle counts, the number of follicles in 3 high power fields (. times.400) per case, were averaged and statistically processed as shown in Table 4.
TABLE 4
| Examples of the invention | Number of follicles (number/high power field) |
| Blank group | 13.24±1.35 |
| Application example 1 | 18.45±0.98* |
| Application example 2 | 18.47±1.28* |
| Application example 3 | 20.89±1.05* |
| Application example 4 | 19.74±0.86* |
| Application example 5 | 19.23±1.56* |
Note: p < 0.05, with significant differences compared to the blank control group; p < 0.001, with a very significant difference compared to the blank control group.
According to the human test data in table 4 above, it can be seen that the shampoo products prepared in application examples 1 to 5 have the effect of significantly improving hair follicles, promoting hair follicle growth, and further promoting hair growth, which corresponds to the test results in test example 1.
To further prove that the shampoo products prepared by the present invention can improve the hair follicle status and promote hair growth, the above experiment was performed on the shampoo products prepared in application examples 6 to 14, and 45 rats with smooth and non-damaged skin in the depilated area were randomly selected into 9 groups of 5 rats, and the results are shown in table 5 below.
TABLE 5
| Examples of the invention | Number of follicles (number/high power field) |
| Blank group | 13.24±1.35 |
| Application example 6 | 23.63±1.10** |
| Application example 7 | 23.12±1.14** |
| Application example 8 | 23.05±1.08** |
| Application example 9 | 23.96±1.63** |
| Application example 10 | 24.93±1.04** |
| Application example 11 | 24.55±1.12** |
| Application example 12 | 28.42±0.86** |
| Application example 13 | 24.31±0.94** |
| Application example 14 | 24.38±1.06** |
Note: p < 0.05, with significant differences compared to the blank control group; p < 0.001, with a very significant difference compared to the blank control group.
According to the human test data in table 5 above, it can be seen that the shampoo products prepared in examples 6-12 have a certain hair follicle improving effect, and can promote hair follicle growth, but have a hair follicle promoting effect, but are not as good as the hair shampoo products prepared in example 12, that is, the shampoo products are prepared by controlling the microbial composition to be the fermentation liquid obtained when bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus are in a mass ratio of 5:3:1, adding the mixture of sodium N-acyl glutamate and amidopropyl amine oxide in a mass ratio of 2:1, and controlling the mass ratio of the rice-washing water fermentation product to the plant extract to be 1:6, so that the shampoo products have a better hair follicle improving effect and can promote hair follicle growth.
To further prove that the shampoo products prepared according to the present invention can improve the hair follicle status and promote hair growth, the above experiments were performed on the shampoo products prepared according to comparative examples 1 to 6, and 30 rats with smooth and non-damaged hair removal area were randomly selected into 6 groups of 5 rats, and the results are shown in table 6 below.
TABLE 6
Note: p < 0.05, with significant differences compared to the blank control group; p < 0.001, with a very significant difference compared to the blank control group.
According to the detection data in the table 6, when the mass ratio of the changed microorganisms is not within the protection range of the invention, the hair washing product prepared by the method has obviously reduced hair follicle growth promoting effect, and has the same effect as the application example 3; changing the mass ratio of the components in the surfactant can obviously influence the effects of resisting alopecia and promoting hair growth of the shampoo product, because the type of the surfactant can obviously influence the absorption of the hair on the shampoo product, the effect of promoting the hair follicle growth can be obviously influenced; the change of the mass ratio of the rice washing water fermentation product to the plant extract can affect the overall quality of the product to a certain extent, thereby affecting the effect of promoting the growth of hair follicles.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (3)
1. A shampoo product, which is characterized in that: comprises 5-10 parts of rice washing water fermentation product of microbial composition, 10-30 parts of surfactant, 30-50 parts of plant extract, 2-5 parts of essence and 10-20 parts of deionized water;
the microbial composition is Bifidobacterium longum, Lactobacillus acidophilus and Pediococcus pentosaceus in a mass ratio of 3-5:2-3: 1;
the Bifidobacterium longum（Bifidobacterium longum）The preservation number of the strain is CGMCC No.20684, the preservation time is 09 and 21 days in 2020, and the strain is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the strain are as follows: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the lactobacillus acidophilus（Lactobacillus acidophilus）The preservation number of the strain is CGMCC No.20683, the preservation time is 09 and 21 days in 2020, and the strain is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the strain are as follows: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the pediococcus pentosaceus（Pediococcuspentosaceus）The preservation number of the strain is CGMCC No.20682, the preservation time is 09 and 21 days in 2020, and the strain is preserved in China general microbiological culture Collection center (CGMCC), and the addresses of the strain are as follows: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences;
the surfactant is a mixture of N-acyl sodium glutamate and amidopropyl amine oxide in a mass ratio of 1-3: 1;
the mass ratio of the rice washing water fermentation product to the plant extract is 1: 3-10;
the plant extract comprises a catechu extract and a kelp extract in a mass ratio of 1: 1.
2. Shampoo product according to claim 1, characterised in that the microbial composition is bifidobacterium longum, lactobacillus acidophilus and pediococcus pentosaceus in a mass ratio of 5:3: 1.
3. The shampoo product of claim 1 wherein the rice-washing water fermentation product is a fermentation lysate or/and fermentation exosomes.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103876986A (en) * | 2014-04-02 | 2014-06-25 | 谢妮妮 | Plant essence hair loss-stopping conditioning liquid shampoo and preparation method thereof |
| CN104473859A (en) * | 2014-12-09 | 2015-04-01 | 青岛七好营养科技有限公司 | Liquid shampoo and preparation method thereof |
| CN106667861A (en) * | 2015-11-06 | 2017-05-17 | 焦秀英 | Rice washing water liquid shampoo |
| CN107362104A (en) * | 2017-09-04 | 2017-11-21 | 上海张苍生物科技有限公司 | A kind of alopecia-stopping, the leaves shampoo of hair tonic |
| CN108670916A (en) * | 2018-05-23 | 2018-10-19 | 玄启发 | A kind of rice washing water shampoo |
| CN109136129A (en) * | 2018-08-27 | 2019-01-04 | 南昌大学 | One plant of lactobacillus acidophilus NCU426 |
| CN110787110A (en) * | 2019-12-09 | 2020-02-14 | 广州栋方生物科技股份有限公司 | Composition with hair care effect and preparation method thereof |
| CN111956580A (en) * | 2020-09-15 | 2020-11-20 | 广州科盈化妆品有限公司 | Anti-hair loss shampoo and preparation method thereof |
-
2020
- 2020-12-11 CN CN202011456715.XA patent/CN112442466B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103876986A (en) * | 2014-04-02 | 2014-06-25 | 谢妮妮 | Plant essence hair loss-stopping conditioning liquid shampoo and preparation method thereof |
| CN104473859A (en) * | 2014-12-09 | 2015-04-01 | 青岛七好营养科技有限公司 | Liquid shampoo and preparation method thereof |
| CN106667861A (en) * | 2015-11-06 | 2017-05-17 | 焦秀英 | Rice washing water liquid shampoo |
| CN107362104A (en) * | 2017-09-04 | 2017-11-21 | 上海张苍生物科技有限公司 | A kind of alopecia-stopping, the leaves shampoo of hair tonic |
| CN108670916A (en) * | 2018-05-23 | 2018-10-19 | 玄启发 | A kind of rice washing water shampoo |
| CN109136129A (en) * | 2018-08-27 | 2019-01-04 | 南昌大学 | One plant of lactobacillus acidophilus NCU426 |
| CN110787110A (en) * | 2019-12-09 | 2020-02-14 | 广州栋方生物科技股份有限公司 | Composition with hair care effect and preparation method thereof |
| CN111956580A (en) * | 2020-09-15 | 2020-11-20 | 广州科盈化妆品有限公司 | Anti-hair loss shampoo and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| Microbial, saccharifying and antioxidant properties of an Indian rice;Kuntal Ghosh等;《Food chemistry》;20150201;全文 * |
| 一株嗜酸乳杆菌的分离与鉴定;吴家鑫等;《生物加工工程》;20191130;第17卷(第6期);摘要，第577页左栏第3段，第579页右栏倒数第3段 * |
| 傣族发酵淘米水的初步研究;杨海英等;《食品工业科技》;20171201;第38卷(第23期);全文 * |
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