JP7138987B1 - Bacterial flora improving agent and its use - Google Patents
Bacterial flora improving agent and its use Download PDFInfo
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- JP7138987B1 JP7138987B1 JP2021212468A JP2021212468A JP7138987B1 JP 7138987 B1 JP7138987 B1 JP 7138987B1 JP 2021212468 A JP2021212468 A JP 2021212468A JP 2021212468 A JP2021212468 A JP 2021212468A JP 7138987 B1 JP7138987 B1 JP 7138987B1
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- bacterial flora
- tea seeds
- tea
- skin
- improving agent
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Abstract
【課題】黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有し、表皮ブドウ球菌優位の細菌叢の形成に貢献し得る細菌叢改善剤を提供する。【解決手段】ここで開示される細菌叢改善剤は、鬼皮を実質的に含有しない茶種子に由来する選択的抗菌成分を含有する。本発明者が行った実験によると、茶種子（チャノキの種子）から鬼皮を除去した茶種子（薄皮、胚乳、子葉および胚軸）は、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を発揮することが確認されている。このため、この鬼皮を除去した茶種子に由来する選択的抗菌成分を含有する細菌叢改善剤は、表皮ブドウ球菌優位の細菌叢を形成することに貢献できる。【選択図】なし[Problem] To provide a bacterial flora-improving agent that has selective antibacterial properties such that the growth of Staphylococcus aureus is inhibited while the growth of Staphylococcus epidermidis is less likely to be inhibited, and that can contribute to the formation of a bacterial flora dominated by Staphylococcus epidermidis. offer. Kind Code: A1 A bacterial flora improving agent disclosed herein contains a selective antibacterial component derived from tea seeds that are substantially free of black skin. According to experiments conducted by the present inventor, tea seeds (thin skin, endosperm, cotyledon and hypocotyl) from which the demon skin was removed from tea seeds (tea seeds) inhibited the growth of Staphylococcus aureus, while epidermis grapes It has been confirmed that it exerts a selective antibacterial property that hardly inhibits the growth of cocci. Therefore, a bacterial flora-improving agent containing a selective antibacterial component derived from tea seeds from which the husk has been removed can contribute to the formation of a bacterial flora dominated by S. epidermidis. [Selection figure] None
Description
ここに開示される技術は、細菌叢改善剤およびその利用に関する。具体的には、チャノキに由来する抗菌成分を含む細菌叢改善剤と、当該細菌叢改善剤を利用した各種技術に関する。 TECHNICAL FIELD The technology disclosed herein relates to a bacterial flora improving agent and its use. Specifically, the present invention relates to a bacterial flora-improving agent containing an antibacterial component derived from tea tree, and various techniques using the bacterial flora-improving agent.
チャノキ（Camellia sinensis）は、ツバキ科ツバキ属の植物であり、その葉（茶葉）からの抽出物は、茶系飲料（緑茶、紅茶等）として広く飲用されている。また、茶葉は、様々な細菌に対して抗菌作用を有していることが確認されており、食品以外の分野でも広く使用されている。例えば、特許文献１には、茶葉抽出物を不織布に付与することによって抗菌性不織布を作製する技術が開示されている。また、特許文献２には、紅茶からの抽出物を、コリネバクテリウム属（Corynebacterium）菌やミクロコッカス属（Micrococcus）菌に対する抗菌剤として使用する技術が開示されている。
Camellia sinensis is a plant belonging to the genus Camellia of the family Theaceae, and extracts from its leaves (tea leaves) are widely consumed as tea-based beverages (green tea, black tea, etc.). In addition, it has been confirmed that tea leaves have an antibacterial effect against various bacteria, and they are widely used in fields other than food. For example,
また、近年では、茶葉だけでなく、チャノキの実（茶実）も様々な分野で利用されている。例えば、茶実には多くの脂質成分が含まれており、これを抽出（搾油）することによって茶実油を製造できる。かかる茶実油は、食品、化粧品などの様々な分野で使用されている。例えば、特許文献３には、茶実から茶実油を抽出する方法が開示されている。
In recent years, not only tea leaves but also tea tree seeds (tea seeds) have been used in various fields. For example, tea seed contains many lipid components, and tea seed oil can be produced by extracting (extracting) this. Such tea seed oil is used in various fields such as foods and cosmetics. For example,
上述の通り、茶葉は、様々な細菌に対する抗菌作用を有しているため、抗菌剤として広く使用されている。しかしながら、この茶葉は、抗菌作用が強くなりすぎる傾向があり、人体の細菌叢（皮膚細菌叢、腸内細菌叢等）のバランスを崩す原因になり得る。具体的には、人間の皮膚には、表皮ブドウ球菌（Staphylococcus epidermidis）や、黄色ブドウ球菌(Staphylococcus aureus)などを含む皮膚細菌叢が形成されている。これらのうち、表皮ブドウ球菌は、非病原性であり、グリセリンの生産などによって皮膚の保護に貢献するため善玉菌とみなされている。一方、黄色ブドウ球菌は、病原性が高く、炎症や化膿などの原因になり得るため悪玉菌とみなされている。このため、皮膚細菌叢を改善して健康な肌環境を得るには、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有した薬剤の投与が求められる。しかし、茶葉は、黄色ブドウ球菌の生育を阻害するだけでなく、表皮ブドウ球菌の生育も阻害してしまうため、善玉菌の減少による肌荒れの原因になる可能性がある。 As described above, tea leaves are widely used as an antibacterial agent because they have an antibacterial effect against various bacteria. However, the tea leaves tend to have too strong an antibacterial effect and can cause an imbalance in the human body's flora (skin flora, intestinal flora, etc.). Specifically, skin flora including Staphylococcus epidermidis, Staphylococcus aureus, and the like is formed on human skin. Among these, Staphylococcus epidermidis is considered a good bacterium because it is nonpathogenic and contributes to skin protection, such as by producing glycerin. On the other hand, Staphylococcus aureus is considered a bad bacterium because it is highly pathogenic and can cause inflammation, suppuration, and the like. For this reason, in order to improve the skin flora and obtain a healthy skin environment, while inhibiting the growth of Staphylococcus aureus, it is difficult to inhibit the growth of Staphylococcus epidermidis. is required. However, tea leaves not only inhibit the growth of Staphylococcus aureus, but also inhibit the growth of Staphylococcus epidermidis.
ここに開示される技術は、かかる実情を鑑みてなされたものであり、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有し、表皮ブドウ球菌優位の細菌叢の形成に貢献し得る細菌叢改善剤を提供することを目的とする。 The technology disclosed herein has been made in view of such circumstances. An object of the present invention is to provide a bacterial flora-improving agent that can contribute to the formation of cocci-dominant bacterial flora.
本発明者は、上記の目的を達成するための検討を行うに際して、茶実油の原料である茶実に着目した。この茶実は、茶葉ほど強力なものではないが、一定以上の抗菌性を有している。そして、この茶実の抗菌性を研究した結果、茶実の最も外側の殻部分である鬼皮は、茶葉と同様に黄色ブドウ球菌と表皮ブドウ球菌の両方に対して強い抗菌作用を発揮することが分かった。一方で、鬼皮を除去した残りの部分は、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有していることが判明した。ここに開示される細菌叢改善剤は、かかる発見に基づいてなされたものである。 The inventors of the present invention focused on tea seed, which is a raw material of tea seed oil, when conducting studies to achieve the above object. This tea seed is not as strong as tea leaves, but it has a certain level of antibacterial activity. As a result of researching the antibacterial properties of this tea seed, it was found that the demon skin, which is the outermost shell of the tea seed, exerts a strong antibacterial effect against both Staphylococcus aureus and Staphylococcus epidermidis like tea leaves. I found out. On the other hand, it was found that the remaining part from which the demon skin was removed has selective antibacterial properties such that it inhibits the growth of Staphylococcus aureus but hardly inhibits the growth of Staphylococcus epidermidis. The microflora-improving agents disclosed herein were made based on such findings.
すなわち、ここに開示される細菌叢改善剤は、鬼皮を実質的に含有しない茶種子に由来する選択的抗菌成分を含有する。かかる細菌叢改善剤を用いることによって、黄色ブドウ球菌の生育が阻害される一方で、表皮ブドウ球菌の生育が阻害されにくくなるため、表皮ブドウ球菌優位の細菌叢の形成に貢献することができる。 That is, the bacterial flora-improving agent disclosed herein contains a selective antibacterial component derived from tea seeds that do not substantially contain demon skin. By using such a bacterial flora-improving agent, the growth of Staphylococcus aureus is inhibited, while the growth of Staphylococcus epidermidis is less likely to be inhibited, thus contributing to the formation of a bacterial flora dominated by Staphylococcus epidermidis.
ここに開示される細菌叢改善剤の一態様では、選択的抗菌成分として、鬼皮を実質的に含有しない茶種子から抽出した抽出物を含有する。これによって、好適な選択的抗菌性を有する細菌叢改善剤を容易に提供できる。なお、上記抽出物としては、鬼皮を実質的に含有しない茶種子から抽出した水溶性成分や油分（典型的には搾油処理を行って得られた成分）などが挙げられる。これらの抽出物を含む細菌叢改善剤は、選択的抗菌性をより適切に発揮できる。 One aspect of the agent for improving the bacterial flora disclosed herein contains an extract extracted from tea seeds that do not substantially contain demon skin as a selective antibacterial component. This makes it possible to easily provide a bacterial flora-improving agent having suitable selective antibacterial properties. Examples of the above extracts include water-soluble components and oil components (typically components obtained by oil extraction) extracted from tea seeds that do not substantially contain demon skin. Bacterial flora-improving agents containing these extracts can better exert selective antibacterial properties.
ここに開示される細菌叢改善剤の他の態様では、選択的抗菌成分として、鬼皮を実質的に含有しない茶種子の固形分を含有する。このような固形分を選択的抗菌成分として使用した場合でも、好適な選択的抗菌性を有する細菌叢改善剤を提供できる。なお、上記固形分としては、搾油後の茶種子の粉砕物や、未搾油の茶種子の粉砕物が挙げられる。これらの固形物を含む細菌叢改善剤は、選択的抗菌性をより適切に発揮できる。 Another embodiment of the bacterial flora-improving agent disclosed herein contains tea seed solids that are substantially free of bark as a selective antibacterial component. Even when such a solid content is used as a selective antibacterial ingredient, a bacterial flora improving agent having suitable selective antibacterial properties can be provided. In addition, as said solid content, the ground material of the tea seed after oil expression, and the ground material of the tea seed of non-oil extraction are mentioned. Bacterial flora-improving agents containing these solids can more appropriately exhibit selective antibacterial properties.
ここに開示される細菌叢改善剤の一態様では、薬学的に許容可能な担体をさらに含有する。これによって、他の製品への添加が容易な細菌叢改善剤を得ることができる。 One aspect of the bacterial flora improving agent disclosed herein further comprises a pharmaceutically acceptable carrier. This makes it possible to obtain a microflora-improving agent that is easy to add to other products.
なお、上記担体を含有する態様では、担体が、常水、精製水、蒸留水、注射用水、リン酸緩衝生理食塩水、生理食塩水、アルコール水溶液、多価アルコール、液状油からなる群から選択される少なくとも一種であることが好ましい。これらの担体によると、鬼皮を実質的に含有しない茶種子の細菌叢改善作用をより好適に発揮させることができる。 In the embodiment containing the carrier, the carrier is selected from the group consisting of normal water, purified water, distilled water, water for injection, phosphate buffered saline, saline, aqueous alcohol solution, polyhydric alcohol, and liquid oil. is preferably at least one of By using these carriers, it is possible to more preferably exhibit the effect of improving the bacterial flora of tea seeds that do not substantially contain demon skin.
また、ここに開示される細菌叢改善剤は、チャノキ由来の天然成分を使用しているため、人体に接触し得る製品に好適に使用できる。このような製品の一例として、皮膚外用剤が挙げられる。 In addition, since the agent for improving bacterial flora disclosed herein uses a natural ingredient derived from tea tree, it can be suitably used for products that may come into contact with the human body. An example of such a product is a topical skin preparation.
また、ここに開示される技術の他の側面として、所定の対象物の細菌叢を改善する細菌叢改善方法が提供される。かかる細菌叢改善方法の対象物は、黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）と表皮ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ｅｐｉｄｅｒｍｉｄｉｓ）とを含む細菌叢を有している。そして、この細菌叢改善方法は、細菌叢における黄色ブドウ球菌に対する表皮ブドウ球菌の存在比を向上させるように、上述した細菌叢改善剤を対象物に供給する。これによって、鬼皮を実質的に含有しない茶種子が有する選択的抗菌性を利用し、表皮ブドウ球菌優位の細菌叢が形成されることを促進できる。 In addition, as another aspect of the technology disclosed herein, a method for improving bacterial flora is provided for improving the bacterial flora of a given target. An object of such a bacterial flora improvement method has a bacterial flora including Staphylococcus aureus and Staphylococcus epidermidis. Then, this bacterial flora improving method supplies the above-mentioned bacterial flora improving agent to an object so as to improve the abundance ratio of Staphylococcus epidermidis to Staphylococcus aureus in the bacterial flora. This makes it possible to utilize the selective antibacterial properties of tea seeds that do not substantially contain the onigiri and promote the formation of a bacterial flora dominated by Staphylococcus epidermidis.
以下、ここに開示される技術の好適な一実施形態を説明する。なお、本明細書において特に言及している事項以外の事柄であって、ここに開示される技術の実施に必要な事柄は、当該分野における従来技術に基づく当業者の設計事項として把握され得る。ここに開示される技術は、本明細書に記載した内容と当該分野における技術知識とに基づいて実施することができる。なお、本明細書において数値範囲を「Ａ～Ｂ」と示す場合、「Ａ以上Ｂ以下（従ってＡを上回りＢを下回る範囲を包含する）」を意味するものとする。 A preferred embodiment of the technology disclosed herein will be described below. Matters other than those specifically referred to in this specification, and matters necessary for implementing the technology disclosed herein, can be grasped as design matters by a person skilled in the art based on the prior art in the relevant field. The technology disclosed herein can be implemented based on the content described in this specification and technical knowledge in the relevant field. In this specification, when a numerical range is indicated as "A to B", it means "A or more and B or less (thus including a range above A and below B)".
１．細菌叢改善剤
以下、ここに開示される細菌叢改善剤について説明する。
1. Bacterial flora improving agent The bacterial flora improving agent disclosed herein will be described below.
（１）作用対象
ここに開示される細菌叢改善剤は、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有している。このような細菌叢改善剤を対象物（人体の皮膚など）に供給することによって、当該対象物の細菌叢における黄色ブドウ球菌が占める割合のみを減らし、表皮ブドウ球菌優位の細菌叢に改善することができる。
(1) Target of Action The bacterial flora-improving agent disclosed herein has selective antibacterial properties such that it inhibits the growth of Staphylococcus aureus but hardly inhibits the growth of Staphylococcus epidermidis. By supplying such a bacterial flora-improving agent to an object (human skin, etc.), only the ratio of Staphylococcus aureus in the bacterial flora of the object is reduced, and the bacterial flora is improved to S. epidermidis dominant. can be done.
なお、本明細書における「抗菌作用」とは、殺菌作用や制菌作用を包含する概念である。すなわち、特定の細菌の生菌数を減少させる作用だけでなく、特定の細菌の増殖スピードを抑制する作用が確認された場合も「抗菌作用」を有しているとみなされる。そして、本明細書における「選択的抗菌性」は、黄色ブドウ球菌に対する抗菌作用が相対的に強く、表皮ブドウ球菌に対する抗菌作用が相対的に弱いという性質を指すものとする。すなわち、表皮ブドウ球菌に対して一定以上の抗菌作用を有している場合であっても、当該表皮ブドウ球菌に対する抗菌作用よりも強い抗菌作用が黄色ブドウ球菌に対して認められた場合には、「選択的抗菌性を有している」とみなすものとする。なお、「黄色ブドウ球菌」は、Staphylococcus aureus種に属する細菌のことをいい、亜種（Subspecies）は特に限定されない。同様に、「表皮ブドウ球菌」とは、Staphylococcus epidermidis種に属する細菌のことをいい、亜種は特に限定されない。 The term "antibacterial action" as used herein is a concept that includes bactericidal action and bacteriostatic action. In other words, it is considered to have an "antibacterial effect" when not only the effect of reducing the number of viable bacteria of a specific bacterium but also the effect of suppressing the growth speed of the specific bacterium is confirmed. The term "selective antibacterial property" as used herein refers to the property of having relatively strong antibacterial action against Staphylococcus aureus and relatively weak antibacterial action against Staphylococcus epidermidis. That is, even if it has a certain level of antibacterial action against Staphylococcus epidermidis, if a stronger antibacterial action against Staphylococcus epidermidis is observed than the antibacterial action against Staphylococcus epidermidis, shall be deemed to have “selective antibacterial properties”. "Staphylococcus aureus" refers to bacteria belonging to the species Staphylococcus aureus, and subspecies are not particularly limited. Similarly, "Staphylococcus epidermidis" refers to bacteria belonging to the species Staphylococcus epidermidis, without limitation to subspecies.
（２）成分
ここに開示される細菌叢改善剤は、少なくとも、茶種子に由来する選択的抗菌成分を含有する。以下、各成分について説明する。
(2) Component The bacterial flora improving agent disclosed herein contains at least a selective antibacterial component derived from tea seeds. Each component will be described below.
（ａ）選択的抗菌成分
上述の通り、ここに開示される細菌叢改善剤は、茶種子に由来する選択的抗菌成分を含有する。そして、ここに開示される技術では、通常の茶種子ではなく、鬼皮が除去された茶種子に由来する選択的抗菌成分を使用することを特徴とする。詳しくは後述するが、本発明者による実験の結果、茶実を構成する各部分のうち、鬼皮は、黄色ブドウ球菌と表皮ブドウ球菌の両方に対して強い抗菌作用を有している一方で、鬼皮を除いた他の部分は、黄色ブドウ球菌に対してのみ強い抗菌作用を有することが確認されている。従って、鬼皮が除去された茶種子に由来する選択的抗菌成分を含有する細菌叢改善剤は、黄色ブドウ球菌の生育を阻害する一方で、表皮ブドウ球菌の生育を阻害しにくいという選択的抗菌性を有しているため、表皮ブドウ球菌優位の細菌叢の形成に貢献できる。
(a) Selective antimicrobial component As described above, the bacterial flora improving agent disclosed herein contains a selective antimicrobial component derived from tea seeds. The technology disclosed herein is characterized by the use of selective antibacterial components derived from tea seeds from which the husks have been removed, rather than ordinary tea seeds. Although the details will be described later, as a result of experiments by the present inventor, it was found that, among the parts that make up the tea seed, demon skin has a strong antibacterial effect against both Staphylococcus aureus and Staphylococcus epidermidis. It has been confirmed that the parts other than the demon skin have a strong antibacterial effect only against Staphylococcus aureus. Therefore, a bacterial flora-improving agent containing a selective antibacterial component derived from tea seeds from which the demon skin has been removed inhibits the growth of Staphylococcus aureus, while it is a selective antibacterial agent that hardly inhibits the growth of Staphylococcus epidermidis. Since it has a property, it can contribute to the formation of S. epidermidis-dominant bacterial flora.
なお、本明細書における「茶種子」とは、茶実（チャノキの種子）のことをいう。より具体的には、「チャノキ」は、ツバキ科ツバキ属チャノキ種（Camellia sinensis）の植物のことを指す。そして、この茶種子は、胚乳と子葉と胚軸から構成される茶種子胚と、鬼皮と薄皮から構成される茶実殻とを備えている。すなわち、本明細書における「鬼皮を含有しない茶種子」とは、茶種子の胚乳と子葉と胚軸と薄皮のことをいう。 The term "tea seed" as used herein refers to tea seed (tea seed). More specifically, "camellia" refers to a plant belonging to the Camellia sinensis species of the genus Camellia of the family Theaceae. This tea seed comprises a tea seed embryo composed of endosperm, cotyledon and hypocotyl, and a tea seed husk composed of husk and thin skin. That is, the term "tea seed containing no demon skin" as used herein refers to the endosperm, cotyledon, hypocotyl and pellicle of the tea seed.
そして、上述の通り、茶種子の鬼皮は、黄色ブドウ球菌だけでなく、表皮ブドウ球菌に対しても強い抗菌作用を有しているため、対象物の細菌叢を表皮ブドウ球菌優位に改善することを阻害する要因になり得る。このため、ここに開示される細菌叢改善剤では、鬼皮を実質的に含有しない茶種子に由来する成分が選択的抗菌成分として使用される。ここで、本明細書における「鬼皮を実質的に含有していない」とは、鬼皮の意図的な添加が行われていないことを指す。換言すると、ここに開示される技術では、選択的抗菌成分を得る際の原料として、鬼皮が除去された茶種子を使用する。但し、鬼皮の一部と解釈され得る材料が原料や製造工程等に由来して不可避的かつ微量に含まれるような場合は、本明細書における「鬼皮を実質的に含有していない」の概念に包含される。例えば、鬼皮以外の茶種子の含有量を１００ｗｔ％としたときの茶実殻の含有量が５ｗｔ％以下（好ましくは１ｗｔ％以下、より好ましくは０．５ｗｔ％以下、特に好ましくは０．１ｗｔ％以下）である場合、「鬼皮を実質的に含有していない（鬼皮の意図的な添加を行っていない）」とみなすことができる。 And, as described above, the demon skin of tea seeds has a strong antibacterial effect not only against Staphylococcus aureus but also against Staphylococcus epidermidis, so it improves the bacterial flora of the target to favor Staphylococcus epidermidis. It can be a factor that hinders Therefore, in the bacterial flora-improving agent disclosed herein, a component derived from tea seeds, which does not substantially contain the bark, is used as a selective antibacterial component. Here, the phrase "substantially does not contain demon skin" in the present specification means that intentional addition of demon skin is not performed. In other words, the technology disclosed herein uses tea seeds from which the husk has been removed as a raw material for obtaining the selective antibacterial component. However, if a material that can be interpreted as part of the demon skin is unavoidably contained in a trace amount due to raw materials, manufacturing processes, etc., "does not substantially contain demon skin" in this specification. subsumed in the concept of For example, when the content of tea seeds other than demon skin is 100 wt%, the content of tea husks is 5 wt% or less (preferably 1 wt% or less, more preferably 0.5 wt% or less, particularly preferably 0.1 wt% % or less), it can be regarded as "substantially free of demon skin (no intentional addition of demon skin)".
また、本明細書における「選択的抗菌成分」は、鬼皮を実質的に含有しない茶種子に由来した成分であれば特に限定されず、種々の形態を採ることができる。選択的抗菌成分の形態の一例として、鬼皮を実質的に含有しない茶種子から抽出された抽出物が挙げられる。この抽出物の一例として、水溶性成分が挙げられる。かかる水溶性成分を抽出する手段は、特に限定されないが、例えば、鬼皮を実質的に含有しない茶種子を水系媒体に分散させた分散液の上澄みを取得するという手段を採用することができる。また、抽出物の他の例として、油分が挙げられる。この油分を抽出する手段の一例として、鬼皮を実質的に含有しない茶種子に対して搾油処理を行うという手段を採用することができる。また、選択的抗菌成分の形態は、上述した抽出物に限定されず、固形分であってもよい。かかる固形分の典型的として、鬼皮を実質的に含有しない茶種子を粉砕した粉砕物が挙げられる。また、このときの粉砕物は、搾油後の茶種子を粉砕したものであってもよいし、未搾油の茶種子を粉砕したものであってもよい。何れの形態においても適切な選択的抗菌性が発揮されることが、本発明者らによる実験において確認されている。 In addition, the "selective antibacterial component" in the present specification is not particularly limited as long as it is a component derived from tea seeds that do not substantially contain demon skin, and can take various forms. An example of a form of selective antimicrobial component is an extract extracted from tea seeds that are substantially free of demon skin. An example of this extract includes water-soluble components. The means for extracting such a water-soluble component is not particularly limited, but for example, a means of obtaining the supernatant of a dispersion in which tea seeds that do not substantially contain demon skin are dispersed in an aqueous medium can be adopted. Another example of the extract is oil. As an example of means for extracting this oil, a means of subjecting tea seeds that do not substantially contain demon skin to oil expression can be employed. Also, the form of the selective antibacterial component is not limited to the above-described extract, and may be a solid content. A typical example of such a solid content is a pulverized product obtained by pulverizing tea seeds that do not substantially contain demon skin. Moreover, the pulverized material at this time may be obtained by pulverizing tea seeds after oil expression, or may be pulverized tea seeds that have not been oil-extracted. Experiments by the present inventors have confirmed that appropriate selective antibacterial properties are exhibited in any form.
（ｂ）担体
また、細菌叢改善剤は、ここに開示される技術による効果が失われない範囲内（すなわち、選択的抗菌成分を発揮し得る範囲内）で、薬学（医薬）上許容され得る程度の安全性を有した担体を含んでいることが好ましい。なお、当該担体の具体的な態様は、細菌叢改善剤の用途や形態に応じて適宜異なり得る。例えば、液体の細菌叢改善剤を得る場合には、上記担体として、常水、精製水、蒸留水、注射用水、リン酸緩衝生理食塩水（ＰＢＳ）、生理食塩水などの水系分散媒を使用することが好ましい。また、液体の担体の他の例として、適当な濃度のアルコール（エタノール等）水溶液、多価アルコール、液状油等が挙げられる。但し、ここに開示される細菌叢改善剤は、上述の担体を含有しない態様を包含し得る。例えば、固形分（粉砕物）を固形の製品（石鹸など）に添加する場合や、搾油した油分をそのまま使用する場合などにおいては、担体を添加しなくてもよい。
(b) carrier In addition, the bacterial flora-improving agent is pharmaceutically (pharmaceutical) acceptable within a range in which the effects of the technology disclosed herein are not lost (i.e., within a range in which a selective antibacterial component can be exerted) It preferably contains a carrier with a certain degree of safety. In addition, the specific aspect of the carrier may vary as appropriate depending on the application and form of the bacterial flora improving agent. For example, when obtaining a liquid bacterial flora-improving agent, an aqueous dispersion medium such as ordinary water, purified water, distilled water, water for injection, phosphate buffered saline (PBS), and physiological saline is used as the carrier. preferably. Other examples of liquid carriers include alcohol (ethanol, etc.) aqueous solutions of appropriate concentrations, polyhydric alcohols, liquid oils, and the like. However, the bacterial flora-improving agents disclosed herein may include embodiments that do not contain the aforementioned carrier. For example, when a solid content (pulverized product) is added to a solid product (soap, etc.), or when an extracted oil content is used as it is, a carrier may not be added.
（ｃ）添加剤
また、ここに開示される細菌叢改善剤は、選択的抗菌性を大きく損なわない限りにおいて、細菌叢改善剤や抗菌剤に使用され得る従来公知の添加剤を含有していてもよい。このような添加剤の一例として、黄色ブドウ球菌の生育を阻害する抗菌成分が挙げられる。この種の抗菌成分としては、各種抗生物質、フェノキシエタノール、メチルパラベン、エチルパラベン、ブチルパラベン、プロピルパラベン、レゾルシン、サリチル酸、デヒドロ酢酸、ヘキサクロロフェン、塩化ベンザルコニウム、イソプロピルメチルフェノール、アルカンジオールなどが挙げられる。また、抗菌成分の他の例として、植物由来の天然抗菌成分が挙げられる。かかる植物由来の成分の一例として、オレガノ抽出物、カラシ抽出物、カンゾウ抽出物、クローブ抽出物、クワ抽出物、チャ抽出物、リンゴ抽出物、シソ抽出物、ショウガ抽出物、セイヨウワサビ抽出物、セージ抽出物、トウガラシ抽出物、ニンニク抽出物、ピメンタ抽出物、ブドウ抽出物、プロポリス抽出物、ペパー抽出物、ホコッシ抽出物、モウソウチク抽出物、ユッカフォーム抽出物、ローズマリー抽出物、ワサビ抽出物などが挙げられる。また、添加剤の他の例として、表皮ブドウ球菌の生育を促進する栄養成分が挙げられる。この種の栄養成分の一例として、グルコース、スクロース、水飴、デキストリン、澱粉、グリセロール、糖蜜等の炭素源が挙げられる。また、栄養成分の他の例として、ポリペプトン、大豆粉、小麦胚芽、コーン・スティープ・リカー、綿実粕、肉エキス、マルトエキス、イーストエキス、硫酸アンモニウム、硝酸ナトリウム、尿素等の窒素源が挙げられる。なお、抗菌成分や栄養成分を添加する場合には、これらの成分が黄色ブドウ球菌と表皮ブドウ球菌の生育に与える影響を考慮した上で、選択的抗菌成分の含有量を調節することが好ましい。
(c) Additives In addition, the bacterial flora improving agent disclosed herein contains conventionally known additives that can be used for bacterial flora improving agents and antibacterial agents as long as they do not significantly impair the selective antibacterial properties. good too. An example of such an additive is an antimicrobial component that inhibits the growth of Staphylococcus aureus. Antibacterial ingredients of this kind include various antibiotics, phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, resorcinol, salicylic acid, dehydroacetic acid, hexachlorophene, benzalkonium chloride, isopropylmethylphenol, alkanediols, and the like. . Other examples of antibacterial ingredients include plant-derived natural antibacterial ingredients. Examples of such plant-derived ingredients include oregano extract, mustard extract, licorice extract, clove extract, mulberry extract, tea extract, apple extract, perilla extract, ginger extract, horseradish extract, Sage extract, Capsicum extract, Garlic extract, Pimenta extract, Grape extract, Propolis extract, Pepper extract, Hokkosi extract, Moso extract, Yucca foam extract, Rosemary extract, Wasabi extract, etc. is mentioned. Other examples of additives include nutritional ingredients that promote the growth of Staphylococcus epidermidis. Examples of this type of nutritional component include carbon sources such as glucose, sucrose, starch syrup, dextrin, starch, glycerol and molasses. Other examples of nutritional components include nitrogen sources such as polypeptone, soy flour, wheat germ, corn steep liquor, cottonseed meal, meat extract, malt extract, yeast extract, ammonium sulfate, sodium nitrate, and urea. . In addition, when adding antibacterial ingredients and nutritional ingredients, it is preferable to adjust the content of selective antibacterial ingredients after considering the effects of these ingredients on the growth of Staphylococcus aureus and Staphylococcus epidermidis.
また、添加剤の他の例として、増粘剤などが挙げられる。かかる増粘剤としては、カラギーナン、デヒドロキサンタンガム、キサンタンガム、スクレロチウムガム、タマリンドガム、グアーガム、ローストビーンガム、ジェランガム、クインスシード、アラビアガム、アルギン酸塩、ペクチン、寒天、デンプン、マンナン、セルロース、キトサン、スクシノグリカン、ヒアルロン酸塩、シロキクラゲ多糖体、カルボキシビニルポリマープロパンジオール、グリセリン、ジグリセリン、ポリグリセリン－４、ポリグリセリン－６、ポリグリセリン－１０、ブチレングリコール、ペンチレングリコール、ソルビトール、マンニトール、グルコース、フルクトース、キシリトール、マルトース、マルチトース、トレハロース、イノシトール、スクロース、グリコシルトレハロース、グリコーゲンなどが挙げられる。これらの増粘剤を添加することによって、細菌叢改善剤が対象物表面に長時間定着しやすくなるため、対象物の細菌叢の改善に貢献することができる。 Further, other examples of additives include thickeners and the like. Such thickeners include carrageenan, dehydroxanthan gum, xanthan gum, sclerotium gum, tamarind gum, guar gum, roasted bean gum, gellan gum, quince seed, gum arabic, alginate, pectin, agar, starch, mannan, cellulose, chitosan. , succinoglycan, hyaluronate, white fungus polysaccharide, carboxyvinyl polymer propanediol, glycerin, diglycerin, polyglycerin-4, polyglycerin-6, polyglycerin-10, butylene glycol, pentylene glycol, sorbitol, mannitol, Glucose, fructose, xylitol, maltose, maltitose, trehalose, inositol, sucrose, glycosyltrehalose, glycogen and the like. Addition of these thickeners makes it easier for the bacterial flora-improving agent to settle on the surface of the object for a long period of time, thereby contributing to the improvement of the bacterial flora of the object.
（４）用途
ここに開示される細菌叢改善剤は、チャノキ由来の天然成分を使用しているため、人体に接触し得る製品に好適に使用できる。このような製品の一例として、皮膚外用剤などが挙げられる。この「皮膚外用剤」は、医薬品、医薬部外品、化粧品などの人間の皮膚に付与し得る製品を包含するものである。この種の皮膚外用剤の具体例として、化粧水、乳液、クリーム、洗顔料、クレンジング料、マッサージ料、パック美容液、シャンプー、リンス、トリートメント、ボディソープ、ハンドソープ、入浴剤、整髪料、養毛料、頭皮料、化粧下地、口紅、ファンデーション、おしろい、アイメイク料、頬化粧料、石鹸、シップ剤、軟膏、香水などが挙げられる。但し、ここに開示される細菌叢改善剤は、表皮ブドウ球菌優位となる細菌叢が要求されるものであれば、皮膚外用剤に限定されず、種々の製品に使用することができる。皮膚外用剤以外の例として、食品や洗剤（食器用洗剤など）が挙げられる。また、ここに開示される細菌叢改善剤は、人体に直接接触する製品以外の用途に使用することもできる。かかる用途の一例として、手摺等の人体と接触する部材を消毒する消毒液などが挙げられる。すなわち、ここに開示される細菌叢改善剤は、黄色ブドウ球菌と表皮ブドウ球菌とを含む細菌叢を有する対象物において、表皮ブドウ球菌優位の細菌叢を形成する細菌叢改善方法を実施する際に広く使用できる。
(4) Uses The agent for improving bacterial flora disclosed herein uses a natural ingredient derived from tea tree, and therefore can be suitably used for products that may come into contact with the human body. Examples of such products include topical skin preparations and the like. This "external preparation for skin" includes products that can be applied to human skin, such as pharmaceuticals, quasi-drugs, and cosmetics. Specific examples of this type of skin preparation for external use include lotions, milky lotions, creams, facial cleansers, cleansers, massage agents, pack serums, shampoos, rinses, treatments, body soaps, hand soaps, bath agents, hair styling agents, and nourishing agents. Examples include hair preparations, scalp preparations, makeup bases, lipsticks, foundations, face powders, eye makeup preparations, cheek preparations, soaps, poultices, ointments, and perfumes. However, the bacterial flora-improving agent disclosed herein is not limited to external skin preparations, and can be used in various products as long as the bacterial flora in which Staphylococcus epidermidis is dominant is required. Examples other than external preparations for skin include food and detergents (such as dishwashing detergents). In addition, the bacterial flora improving agent disclosed herein can also be used for applications other than products that come into direct contact with the human body. An example of such a use is an antiseptic solution for disinfecting members that come into contact with the human body, such as handrails. That is, the bacterial flora-improving agent disclosed herein is used in a subject having a bacterial flora containing Staphylococcus aureus and Staphylococcus epidermidis to form a bacterial flora dominated by Staphylococcus epidermidis. Can be used widely.
また、ここに開示される細菌叢改善剤は、対象物の細菌叢の状態、栄養状態、温度環境、他の添加剤、選択的抗菌成分の形態などの様々な要因を考慮した上で、対象物の細菌叢における黄色ブドウ球菌に対する表皮ブドウ球菌の存在比（表皮ブドウ球菌／黄色ブドウ球菌）が向上するように適宜希釈して使用することが好ましい。また、本発明者による実験の結果、脂質成分を除去した搾油後の茶種子は、未搾油の茶種子と比べて黄色ブドウ球菌に対する抗菌作用が強くなる傾向があることが確認されている。ここに開示される細菌叢改善剤を使用する際には、かかる点も考慮することが好ましい。 In addition, the bacterial flora improving agent disclosed herein is selected after considering various factors such as the state of the bacterial flora of the object, the nutritional state, the temperature environment, other additives, and the form of the selective antibacterial component. It is preferable to dilute it appropriately so as to improve the abundance ratio of Staphylococcus epidermidis to Staphylococcus aureus in the bacterial flora (Staphylococcus epidermidis/Staphylococcus aureus). In addition, as a result of experiments by the present inventor, it has been confirmed that tea seeds after oil extraction from which lipid components have been removed tend to have a stronger antibacterial action against Staphylococcus aureus than tea seeds that have not been oil-extracted. It is preferable to take this point into consideration when using the bacterial flora improving agent disclosed herein.
以下、化粧水を対象とし、選択的抗菌成分として、鬼皮を実質的に含有しない茶種子から抽出した水溶性成分を使用した場合の希釈量の一例を説明する。この場合、化粧水の総量（１００ｗｔ％）に対する選択的抗菌成分（水溶性成分）の濃度が０．０１ｗｔ％以上（好適には０．０５ｗｔ％以上、より好適には０．１ｗｔ％以上、さらに好適には０．２ｗｔ％以上、特に好適には０．２５ｗｔ％以上）となるように細菌叢改善剤を化粧水に添加するとよい。これによって、使用者の皮膚における黄色ブドウ球菌の生育を適切に抑制できる。一方、化粧水における選択的抗菌成分（水溶性成分）の濃度の上限値は、１０ｗｔ％以下でもよく、５ｗｔ％以下でもよい。特に、表皮ブドウ球菌の生育阻害を抑制するという点を考慮すると、選択的抗菌成分（水溶性成分）は、４．５ｗｔ％以下（好適には４．０ｗｔ％以下、より好適には３．５ｗｔ％以下、さらに好適には３．０ｗｔ％以下、特に好適には１．０ｗｔ％以下、例えば０．５ｗｔ％以下）になるように細菌叢改善剤を化粧水に添加するとよい。 An example of the amount of dilution in the case of using a water-soluble component extracted from tea seeds that do not substantially contain demon skin as a selective antibacterial component for lotion will be described below. In this case, the concentration of the selective antibacterial component (water-soluble component) with respect to the total amount (100 wt%) of the lotion is 0.01 wt% or more (preferably 0.05 wt% or more, more preferably 0.1 wt% or more, and further Preferably 0.2 wt % or more, particularly preferably 0.25 wt % or more), the bacterial flora improving agent is added to the lotion. This can appropriately suppress the growth of Staphylococcus aureus on the user's skin. On the other hand, the upper limit of the concentration of the selective antibacterial component (water-soluble component) in the lotion may be 10 wt% or less, or 5 wt% or less. In particular, considering the suppression of growth inhibition of Staphylococcus epidermidis, the selective antibacterial component (water-soluble component) is 4.5 wt% or less (preferably 4.0 wt% or less, more preferably 3.5 wt%) %, more preferably 3.0 wt% or less, particularly preferably 1.0 wt% or less, for example 0.5 wt% or less).
他の例として、クリームを対象とし、選択的抗菌成分として、鬼皮を実質的に含有しない茶種子から抽出した油分を使用した場合の希釈量を説明する。この場合、クリームの総量（１００ｗｔ％）に対する選択的抗菌成分（油分）の濃度が０．０５ｗｔ％以上（好適には０．１ｗｔ％以上、より好適には０．５ｗｔ％以上、さらに好適には１ｗｔ％以上、特に好適には２ｗｔ％以上）となるように細菌叢改善剤を添加するとよい。これによって、クリーム中の黄色ブドウ球菌の生育を適切に抑制できる。一方、クリームにおける選択的抗菌成分（油分）の濃度の上限値は、２０ｗｔ％以下でもよく、１５ｗｔ％以下でもよく、１０ｗｔ％以下でもよい。特に、表皮ブドウ球菌の生育阻害を抑制するという点を考慮すると、選択的抗菌成分（油分）は、５ｗｔ％以下（好適には４．５ｗｔ％以下、より好適には４．０ｗｔ％以下、さらに好適には３．５ｗｔ％以下、特に好適には３．０ｗｔ％以下、例えば１ｗｔ％以下）になるように細菌叢改善剤を化粧水に添加するとよい。 As another example, the amount of dilution will be explained in the case of using an oil extracted from tea seeds that do not substantially contain demon skin as a selective antibacterial component for a cream. In this case, the concentration of the selective antibacterial component (oil) is 0.05 wt% or more (preferably 0.1 wt% or more, more preferably 0.5 wt% or more, more preferably 0.5 wt% or more, more preferably 1 wt % or more, particularly preferably 2 wt % or more), it is preferable to add the bacterial flora improving agent. This can appropriately suppress the growth of Staphylococcus aureus in the cream. On the other hand, the upper limit of the concentration of the selective antibacterial component (oil) in the cream may be 20 wt% or less, 15 wt% or less, or 10 wt% or less. In particular, considering the suppression of growth inhibition of Staphylococcus epidermidis, the selective antibacterial component (oil) is 5 wt% or less (preferably 4.5 wt% or less, more preferably 4.0 wt% or less, and further The bacterial flora-improving agent is preferably added to the lotion so that it is preferably 3.5 wt % or less, particularly preferably 3.0 wt % or less, for example, 1 wt % or less.
なお、細菌の生育度合いは、対象物の細菌叢の状態、栄養状態、温度環境、他の添加剤などの様々な要因によって変動し得る。このため、上述した各用途における選択的抗菌成分の含有量は、特定の条件における含有量の一例であり、ここに開示される細菌叢改善剤の用途を限定することを意図したものではない。 The degree of growth of bacteria may vary depending on various factors such as the state of bacterial flora, nutritional state, temperature environment, and other additives of the object. Therefore, the content of the selective antibacterial component in each application described above is an example of the content under specific conditions, and is not intended to limit the application of the bacterial flora improving agent disclosed herein.
また、細菌叢改善剤を希釈する場合、当該希釈に用いる液体は、ここに開示される技術による効果を大きく損なわない限り特に限定されない。かかる希釈用の液体の一例として、上述した担体として使用され得る液体（常水、精製水、蒸留水、注射用水、リン酸緩衝生理食塩水（ＰＢＳ）、生理食塩水、アルコール水溶液、多価アルコール、液状油など）が挙げられる。 Moreover, when diluting the bacterial flora improving agent, the liquid used for the dilution is not particularly limited as long as it does not significantly impair the effect of the technology disclosed herein. Examples of such liquids for dilution include liquids that can be used as the carriers described above (ordinary water, purified water, distilled water, water for injection, phosphate buffered saline (PBS), physiological saline, aqueous alcohol solution, polyhydric alcohol , liquid oil, etc.).
２．製造方法
次に、ここに開示される細菌叢改善剤を製造する方法の一例について説明する。ここに開示される細菌叢改善剤は、原料準備工程Ｓ１０と、茶実殻除去工程Ｓ２０と、選択的抗菌成分作成工程Ｓ３０とを経て製造される。以下、各々の工程について説明する。なお、ここに開示される細菌叢改善剤は、以下の方法で製造されたものに限定されない。
2. Manufacturing Method Next, an example of a method for manufacturing the bacterial flora-improving agent disclosed herein will be described. The bacterial flora improving agent disclosed herein is manufactured through a raw material preparation step S10, a tea husk removal step S20, and a selective antibacterial component preparation step S30. Each step will be described below. In addition, the bacterial flora improving agent disclosed here is not limited to what was manufactured by the following method.
（１）原料準備工程Ｓ１０
本工程では、選択的抗菌成分の原料となる茶種子を準備する。この茶種子は、茶種子胚（胚乳、子葉および胚軸）が茶実殻（鬼皮および薄皮）に覆われたものである。なお、収穫時の茶実は、通常、殻斗と呼ばれる外殻に収容されている。本工程において準備する茶種子は、この殻斗を割って取り出したものである。
(1) Raw material preparation step S10
In this step, tea seeds are prepared as raw materials for the selective antibacterial component. This tea seed is a tea seed embryo (endosperm, cotyledon and hypocotyl) covered with a tea husk (demon skin and thin skin). It should be noted that the tea seeds at the time of harvesting are usually housed in an outer shell called a hull. The tea seeds prepared in this step are taken out by breaking the shell.
（２）鬼皮除去工程Ｓ２０
本工程では、茶種子から鬼皮を除去する。鬼皮を除去する手段は、特に限定されず、従来公知の方法を特に制限なく使用できる。例えば、上述した特許文献３（特開２０２０－１５２７４９号）に記載されているように、一対のローラ間で茶種子を押圧して鬼皮を粉砕除去する手段などを採用できる。このような粉砕除去を実施すると、外側の硬い鬼皮のみが除去され、茶種子胚の表面に薄皮が残留した鬼皮を実質的に含有しない茶種子が得られる。また、本工程を実施する前に、茶種子に対して乾燥処理を実施してもよい。これによって、余分な水分が除去されるため、鬼皮をより容易に粉砕除去することができる。
(2) Demon skin removal step S20
In this step, the demon skin is removed from the tea seeds. There are no particular restrictions on the means for removing the demon skin, and conventionally known methods can be used without particular limitations. For example, as described in the above-mentioned Patent Document 3 (JP 2020-152749 A), it is possible to adopt means for crushing and removing the peel by pressing tea seeds between a pair of rollers. When such pulverization and removal is carried out, only the outer hard outer skin is removed, and tea seeds substantially free of the outer skin, in which the thin skin remains on the surface of the tea seed embryo, are obtained. In addition, drying treatment may be performed on the tea seeds before performing this step. As a result, excess moisture is removed, so that the demon skin can be pulverized and removed more easily.
（３）選択的抗菌成分作成工程Ｓ３０
本工程では、鬼皮が除去された茶種子から選択的抗菌成分を作成する。本工程における具体的な処理は、特に限定されず、作成予定の選択的抗菌成分の形態に応じた処理を適宜実施することができる。例えば、選択的抗菌成分の形態を「茶種子の粉砕物」とする場合には、鬼皮が除去された茶種子を所望の大きさに粉砕する粉砕処理を実施するとよい。また、かかる粉砕処理を行う前に搾油処理を実施してもよい。この搾油処理によって油分と搾油後の茶種子（固形分）が得られるが、これらは、何れも選択的抗菌成分として使用できることが実験において確認されている。また、選択的抗菌成分として水溶性成分を使用する場合には、粉砕後の茶種子の粉末を担体（例えば水系媒体）に分散させるとよい。これによって、水溶性成分が担体に溶出した細菌叢改善剤を得ることができる。かかる水溶性成分を選択的抗菌成分とする細菌叢改善剤を得る場合には、鬼皮が除去された茶種子の粉砕物を担体に分散させた分散液を調整し、当該分散液に対して加熱処理を実施するとよい。これによって、水溶性成分をより適切に溶出させることができる。本工程は、上述した内容に限定されない。例えば、鬼皮を実質的に含有しない茶種子を圧搾して得られたエキス（油分や水溶性成分の混合物）を選択的抗菌成分として使用することもできる。また、作成した選択的抗菌成分の形態によっては、上述した担体（水系媒体）等と混合してもよい。これによって、取り扱いが容易な細菌叢改善剤を得ることができる。
(3) Selective antibacterial component preparation step S30
In this step, a selective antibacterial ingredient is prepared from tea seeds from which the demon skin has been removed. The specific treatment in this step is not particularly limited, and treatment can be appropriately carried out according to the form of the selective antibacterial component to be produced. For example, when the selective antibacterial component is in the form of "crushed tea seeds", it is advisable to carry out a crushing treatment of crushing the tea seeds from which the outer skin has been removed into a desired size. Moreover, you may implement an oil extraction process before performing this crushing process. Through this oil extraction treatment, oil and tea seeds (solid content) after oil extraction are obtained, and experiments have confirmed that both of these can be used as selective antibacterial components. Further, when a water-soluble component is used as the selective antibacterial component, it is preferable to disperse the tea seed powder after pulverization in a carrier (for example, an aqueous medium). As a result, a bacterial flora-improving agent in which the water-soluble component is eluted into the carrier can be obtained. In the case of obtaining a bacterial flora-improving agent having such a water-soluble component as a selective antibacterial component, a dispersion is prepared by dispersing the pulverized tea seeds from which the outer skin has been removed in a carrier, and the dispersion is Heat treatment is preferably performed. Thereby, the water-soluble component can be eluted more appropriately. This process is not limited to the content described above. For example, an extract (a mixture of oil and water-soluble components) obtained by squeezing tea seeds that do not substantially contain demon skin can also be used as a selective antibacterial component. Moreover, depending on the form of the prepared selective antibacterial component, it may be mixed with the aforementioned carrier (aqueous medium) or the like. This makes it possible to obtain a bacterial flora-improving agent that is easy to handle.
以上、ここに開示される細菌叢改善剤の実施形態について説明した。しかし、上述の説明は、例示にすぎず、請求の範囲を限定することを意図したものではない。すなわち、請求の範囲に記載の技術には、上述した細菌叢改善剤を様々に変更したものが含まれ得る。 The foregoing has described embodiments of the bacterial flora improving agent disclosed herein. However, the above description is exemplary only and is not intended to limit the scope of the claims. That is, the claimed technology may include various modifications of the above-described bacterial flora-improving agent.
［試験例］
次に、ここに開示される細菌叢改善剤に関する試験例を説明する。なお、以下の試験例は、ここに開示される技術を限定することを意図したものではない。
[Test example]
Next, test examples for the bacterial flora improving agent disclosed herein will be described. It should be noted that the following test examples are not intended to limit the technology disclosed herein.
［第１の試験］
本試験では、抗菌材料が異なる複数種類の組成物を作製した。そして、各々の組成物について、表皮ブドウ球菌（Staphylococcus epidermidis）と黄色ブドウ球菌(Staphylococcus aureus)に対する抗菌性を調べ、表皮ブドウ球菌優位の細菌叢を形成するための細菌叢改善剤として使用できるか否かを評価した。
[First test]
In this test, a plurality of compositions with different antibacterial materials were prepared. Then, for each composition, the antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus was examined, and whether it can be used as a bacterial flora improving agent for forming a bacterial flora dominated by Staphylococcus epidermidis. evaluated whether
１．試験区の準備
（１）試験区Ａ：鬼皮を実質的に含有しない茶種子（搾油後）
試験区Ａでは、鬼皮を実質的に含有しない茶種子（搾油後）を抗菌材料として含有する組成物を調製した。具体的には、まず、下記組成のＳＤ培地を調製し、当該ＳＤ培地に１Ｍ水酸化ナトリウム（ＮａＯＨ）を添加してｐＨを７．５に調整した。そして、茶種子に対して鬼皮を除去する処理を行い、鬼皮を実質的に含有しない茶種子を得た。そして、この鬼皮を実質的に含有しない茶種子に搾油処理を行い、搾油後の固形分を乾燥・粉砕して粉砕物を作成した。そして、この粉砕物をＳＤ培地に添加し、オートクレーブを用いて滅菌処理（１２１℃、１５分）を行った後に冷却した。そして、鬼皮を実質的に含有しない茶種子の粉砕物を含むＳＤ培地に対して遠心分離（２５℃、５０００×ｇ、１５分）を行った後に、上澄み液を試験用組成物として採集した。なお、試験区Ａでは、鬼皮を実質的に含有しない茶種子（搾油後）の粉砕物の添加量を０ｗｔ％～２ｗｔ％の範囲で０．５ｗｔ％刻みで異ならせた５種類の試験用組成物（Ａ１～Ａ５）を準備した。
［ＳＤ培地の組成］
ハイポリペプトンＳ（日本製薬株式会社製） ： １５ｇ／Ｌ
塩化ナトリウム ： ５ｇ／Ｌ
1. Preparation of test plots (1) Test plot A: Tea seeds that do not substantially contain demon skin (after oil extraction)
In test area A, a composition was prepared containing tea seeds (after oil extraction) that did not substantially contain demon skin as an antibacterial material. Specifically, first, an SD medium having the following composition was prepared, and 1M sodium hydroxide (NaOH) was added to the SD medium to adjust the pH to 7.5. Then, the tea seeds were subjected to a treatment for removing the demon skin to obtain tea seeds substantially free of the demon skin. Then, the tea seeds substantially free of the demon skin were subjected to an oil extraction treatment, and the solid content after the oil extraction was dried and pulverized to prepare a pulverized product. Then, this pulverized product was added to SD medium, sterilized using an autoclave (121° C., 15 minutes), and then cooled. Then, after centrifugation (25 ° C., 5000 x g, 15 minutes) was performed on the SD medium containing the pulverized tea seeds that did not substantially contain the demon skin, the supernatant was collected as a test composition. . In test group A, the amount of pulverized tea seeds (after oil extraction) that does not substantially contain demon skin was changed in increments of 0.5 wt% in the range of 0 wt% to 2 wt%. Compositions (A1 to A5) were prepared.
[Composition of SD medium]
High Polypeptone S (manufactured by Nihon Pharmaceutical Co., Ltd.): 15 g / L
Sodium chloride: 5g/L
（２）試験区Ｂ：鬼皮を実質的に含有しない茶種子（未搾油）
試験区Ｂでは、鬼皮を実質的に含有しない茶種子（未搾油）を抗菌材料として含む組成物を調製した。具体的には、鬼皮を実質的に含有しない茶種子の粉砕物を作成する前に搾油処理を行わなかった点を除いて、試験区Ａと同じ条件で試験用組成物を準備した。また、試験区Ｂでは、鬼皮を実質的に含有しない茶種子（未搾油）の粉砕物の添加量を０ｗｔ％～２ｗｔ％の範囲で０．５ｗｔ％刻みで異ならせた５種類の試験用組成物（Ｂ１～Ｂ５）を準備した。
(2) Test area B: Tea seeds that do not substantially contain demon skin (unpressed oil)
In Test Group B, a composition containing tea seeds (unpressed oil) substantially free of demon skin as an antibacterial material was prepared. Specifically, a composition for testing was prepared under the same conditions as in test group A, except that the oil extraction treatment was not performed before preparing the pulverized tea seeds that did not substantially contain the demon skin. In addition, in test group B, the amount of pulverized tea seeds (unpressed oil) that does not substantially contain demon skin was changed in increments of 0.5 wt% in the range of 0 wt% to 2 wt%. Compositions (B1-B5) were prepared.
（３）試験区Ｃ：鬼皮を実質的に含有しない茶種子の油分
試験区Ｃでは、鬼皮を実質的に含有しない茶種子から抽出した油分を含む組成物を調製した。具体的には、試験区Ａと同じ条件で、鬼皮を実質的に含有しない茶種子に搾油処理を行い、抽出された油分の方を抗菌材料として採集した。そして、ＳＤ培地と抗菌材料（油分）とが混合した試験用組成物を、試験区Ａ、Ｂと同じ条件で準備した。なお、試験区Ｃでは、鬼皮を実質的に含有しない茶種子の油分の添加量を０ｗｔ％、０．１ｗｔ％、０．２ｗｔ％、０．４ｗｔ％、０．８ｗｔ％、１．６ｗｔ％に設定した６種類の試験用組成物（Ｃ１～Ｃ６）を準備した。
(3) Test Group C: Oil Content of Tea Seeds Substantially Not Containing Demon Skin In Test Group C, a composition containing oil extracted from tea seeds substantially free of demon skin was prepared. Specifically, under the same conditions as test area A, tea seeds that did not substantially contain demon skin were subjected to oil expression treatment, and the extracted oil was collected as an antibacterial material. Then, a test composition in which the SD medium and the antibacterial material (oil) were mixed was prepared under the same conditions as the test groups A and B. In test group C, the amount of tea seed oil added that does not substantially contain demon skin was 0 wt%, 0.1 wt%, 0.2 wt%, 0.4 wt%, 0.8 wt%, 1.6 wt%. Six types of test compositions (C1 to C6) were prepared.
（４）試験区Ｄ：茶葉
試験区Ｄでは、茶葉を抗菌材料とする試験用組成物を調製した。具体的には、ＳＤ培地に乾燥茶葉の粉末を添加して滅菌処理と遠心分離を行い、上澄み液を試験用組成物として採集した。なお、試験区Ｄでは、茶葉を使用した点を除いた条件を試験区Ａと同じ条件に設定した。また、試験区Ｄでは、乾燥茶葉の添加量を０ｗｔ％、０．００１ｗｔ％、０．００３ｗｔ％、０．００７ｗｔ％、０．０１２５ｗｔ％、０．０２５ｗｔ％に設定した６種類の抗菌組成物（Ｄ１～Ｄ６）を準備した。
(4) Test Group D: Tea Leaves In Test Group D, a test composition using tea leaves as an antibacterial material was prepared. Specifically, dried tea leaf powder was added to SD medium, sterilized and centrifuged, and the supernatant was collected as a test composition. In test group D, the conditions were set to the same conditions as in test group A, except that tea leaves were used. In addition, in test group D, six types of antibacterial compositions ( D1 to D6) were prepared.
（５）試験区Ｅ：茶種子（鬼皮未除去）
試験区Ｅでは、茶実を抗菌材料とする試験用組成物を調製した。具体的には、鬼皮を除去していない乾燥茶種子の粉砕物（未搾油）をＳＤ培地に添加して滅菌処理と遠心分離を行い、上澄み液を試験用組成物として採集した。なお、試験区Ｅでは、鬼皮を除去していない点を除いた条件を試験区Ａと同じ条件に設定した。また、試験区Ｅでは、茶種子の粉砕物の添加量を０ｗｔ％～２ｗｔ％の範囲で０．５ｗｔ％刻みで異ならせた５種類の抗菌組成物（Ｅ１～Ｅ５）を準備した。
(5) Test group E: Tea seeds (unremoved demon skin)
In test area E, a test composition was prepared using tea seeds as an antibacterial material. Specifically, crushed dried tea seeds (unsqueezed oil) without peeling were added to SD medium, sterilized and centrifuged, and the supernatant was collected as a test composition. In test group E, the conditions were set to the same conditions as in test group A, except that the demon skin was not removed. In test group E, five types of antibacterial compositions (E1 to E5) were prepared in which the amount of ground tea seeds added was changed in increments of 0.5 wt% in the range of 0 wt% to 2 wt%.
２．抗菌性評価
ここでは、試験例Ａ～Ｅで準備した抗菌組成物について、表皮ブドウ球菌（Staphylococcus epidermidis）と、黄色ブドウ球菌(Staphylococcus aureus)の各々に対する抗菌性を調べた。以下、具体的な手順を説明する。
2. Antibacterial Evaluation Here, the antibacterial properties of the antibacterial compositions prepared in Test Examples AE were examined against Staphylococcus epidermidis and Staphylococcus aureus. Specific procedures will be described below.
（１）表皮ブドウ球菌に対する抗菌性
まず、下記の組成のＬＢ培地を調製し、当該ＬＢ培地に１Ｍ水酸化ナトリウム（ＮａＯＨ）を添加してｐＨを７．５に調整した後に、オートクレーブを用いて滅菌処理（１２１℃、１５分）を行った。そして、このＬＢ培地に表皮ブドウ球菌の種菌を添加し、３７℃で一晩振盪培養（２４０ｒｐｍ）することによって表皮ブドウ球菌の菌液を得た。
［ＬＢ培地の組成］
ハイポリペプトンＳ（日本製薬株式会社製） ： １０ｇ／Ｌ
酵母エキス（オリエンタル酵母工業株式会社製） ： ５ｇ／Ｌ
塩化ナトリウム ： １０ｇ／Ｌ
(1) Antibacterial properties against Staphylococcus epidermidis Sterilization (121°C, 15 minutes) was performed. Then, an inoculum of Staphylococcus epidermidis was added to this LB medium, and cultured overnight at 37° C. with shaking (240 rpm) to obtain a bacterial solution of Staphylococcus epidermidis.
[Composition of LB medium]
High Polypeptone S (manufactured by Nihon Pharmaceutical Co., Ltd.): 10 g / L
Yeast extract (manufactured by Oriental Yeast Co., Ltd.): 5 g / L
Sodium chloride: 10g/L
次に、試験区Ａ～Ｅで準備した抗菌組成物（Ａ１～Ａ５、Ｂ１～Ｂ５、Ｃ１～Ｃ６、Ｄ１～Ｄ６、Ｅ１～Ｅ５）の各々をスピッツ管に４ｍｌ分採集し、上述の表皮ブドウ球菌の菌液を２μｌ添加した。そして、３７℃で１６時間の振盪培養（２４０ｒｐｍ）を実施した後、遠心分離（１００００×ｇ、５分）を行い、沈殿した表皮ブドウ球菌の菌体重量（ｇ／Ｌ）に基づいて各々の試験用組成物の抗菌性を評価した。結果を図１～図５中の「Ｓ．epidermidis」に示す。なお、図１は、試験区Ａ（鬼皮を実質的に含有しない茶種子（搾油後））における抗菌性評価の結果をまとめたグラフである。図２は、試験区Ｂ（鬼皮を実質的に含有しない茶種子（未搾油））における抗菌性評価の結果をまとめたグラフである。図３は、試験区Ｃ（鬼皮を実質的に含有しない茶種子の油分）における抗菌性評価の結果をまとめたグラフである。図４は、試験区Ｄ（茶葉）における抗菌性評価の結果をまとめたグラフである。そして、図５は、第１の試験の試験区Ｅ（茶種子（鬼皮未除去））における抗菌性評価の結果をまとめたグラフである。なお、図１～図５における縦軸は「菌体重量（ｇ／Ｌ）」であり、横軸は「抗菌材料の添加量（ｗｔ％）」である。 Next, 4 ml of each of the antibacterial compositions (A1 to A5, B1 to B5, C1 to C6, D1 to D6, E1 to E5) prepared in Test Groups A to E were collected in a Spitz tube, and the above epidermal grapes were collected. 2 μl of cocci solution was added. Then, after carrying out shaking culture (240 rpm) for 16 hours at 37 ° C., centrifugation (10000 × g, 5 minutes) was performed, and each The antibacterial properties of the test compositions were evaluated. The results are shown in "S. epidermidis" in FIGS. In addition, FIG. 1 is a graph summarizing the results of the antibacterial evaluation in test group A (tea seeds (after oil extraction) that do not substantially contain demon skin). FIG. 2 is a graph summarizing the results of antibacterial evaluation in test group B (tea seeds (unpressed oil) containing substantially no demon skin). FIG. 3 is a graph summarizing the results of antibacterial evaluation in Test Group C (tea seed oil content that does not substantially contain demon skin). FIG. 4 is a graph summarizing the results of antibacterial evaluation in Test Group D (tea leaves). FIG. 5 is a graph summarizing the results of the antibacterial evaluation in the test group E (tea seeds (unremoved of the husk)) of the first test. In FIGS. 1 to 5, the vertical axis is "weight of bacteria (g/L)" and the horizontal axis is "addition amount of antibacterial material (wt%)".
（２）黄色ブドウ球菌に対する抗菌性
次に、上述した「表皮ブドウ球菌に対する抗菌性」と同様の手順に従って、黄色ブドウ球菌に対する抗菌性を評価した。具体的には、上記組成のＬＢ培地で黄色ブドウ球菌を振盪培養することによって黄色ブドウ球菌の菌液を得た。そして、試験区Ａ～Ｅで準備した試験用組成物の各々をスピッツ管に４ｍｌ分採集し、黄色ブドウ球菌の菌液を２μｌ添加した。そして、３７℃で１６時間の振盪培養（２４０ｒｐｍ）を実施した後、遠心分離（１００００×ｇ、５分）を行い、沈殿した黄色ブドウ球菌の菌体重量（ｇ／Ｌ）に基づいて各々の抗菌組成物の抗菌性を評価した。結果を図１～図５中の「Ｓ．aureus」に示す。
(2) Antibacterial property against Staphylococcus aureus Next, the antibacterial property against Staphylococcus aureus was evaluated according to the same procedure as the above-mentioned "Antibacterial property against Staphylococcus epidermidis". Specifically, a suspension of Staphylococcus aureus was obtained by shaking culture of Staphylococcus aureus in the LB medium having the above composition. Then, 4 ml of each of the test compositions prepared in Test Groups A to E was collected in a Spitz tube, and 2 μl of Staphylococcus aureus bacterial solution was added. Then, after performing shaking culture (240 rpm) for 16 hours at 37 ° C., centrifugation (10000 × g, 5 minutes) was performed, and each The antibacterial properties of the antibacterial composition were evaluated. The results are shown in "S. aureus" in FIGS.
３．試験結果
まず、図４に示すように、茶葉を抗菌材料とした試験区Ｄでは、茶葉の添加量をごく微量にしたにもかかわらず、表皮ブドウ球菌と黄色ブドウ球菌の両方の生育が大きく阻害されていた。このことから、茶葉は、悪玉菌である黄色ブドウ球菌だけでなく、善玉菌である表皮ブドウ球菌の生育も大きく阻害してしまうため、表皮ブドウ球菌優位の細菌叢を形成する選択的抗菌成分として使用できないことが分かった。
3. Test results First, as shown in Fig. 4, in test group D using tea leaves as an antibacterial material, the growth of both Staphylococcus epidermidis and Staphylococcus aureus was greatly inhibited even though the amount of tea leaves added was very small. It had been. For this reason, tea leaves greatly inhibit the growth of not only the bad bacteria Staphylococcus aureus but also the good bacteria Staphylococcus epidermidis. found to be unusable.
次に、図５に示すように、茶種子（鬼皮未除去）を抗菌材料とした試験区Ｅでは、茶実の添加量を１．５％にした場合（Ｅ４）に、表皮ブドウ球菌と黄色ブドウ球菌の菌体重量に若干の違いが確認された。しかしながら、図５中のＥ３およびＥ５に示すように、試験区Ｅで使用する茶種子（鬼皮未除去）は、含有量を少し変化させるだけで、選択的抗菌性が失われてしまうため、選択的抗菌成分として機能させることが非常に困難であることが分かった。 Next, as shown in FIG. 5, in test group E using tea seeds (unremoved) as an antibacterial material, when the amount of tea seeds added was 1.5% (E4), Staphylococcus epidermidis and A slight difference was confirmed in the cell weight of Staphylococcus aureus. However, as shown in E3 and E5 in FIG. 5 , the tea seeds (unremoved) used in test group E lose their selective antibacterial properties even when the content is slightly changed. It has been found to be very difficult to function as a selective antimicrobial component.
一方、図１～図３に示すように、鬼皮を除去した茶種子（鬼皮を実質的に含有しない茶種子）に由来する抗菌材料を含む試験区Ａ～Ｃでは、表皮ブドウ球菌と黄色ブドウ球菌との間の菌体重量の差が大きくなりやすいことが確認された。このことから、鬼皮を実質的に含有しない茶種子は、善玉菌である表皮ブドウ球菌の生育を阻害しにくく、悪玉菌である黄色ブドウ球菌の生育を阻害する選択的抗菌性を有していることが分かった。なお、試験区Ａ、Ｂを比較すると、搾油後の鬼皮を実質的に含有しない茶種子を使用している試験例Ａの方が、黄色ブドウ球菌に対する抗菌性が強いことが分かった。また、鬼皮を除去した茶種子から水系媒体に水溶性成分を溶出させた場合（試験区Ａ、Ｂ）であっても、鬼皮を除去した茶種子を搾油して得た油分を添加した場合（試験区Ｃ）であっても、適切な選択的抗菌性を有する細菌叢改善剤が得られることが分かった。 On the other hand, as shown in FIGS. 1 to 3, in test groups A to C containing antibacterial materials derived from tea seeds from which demon skin was removed (tea seeds that do not substantially contain demon skin), Staphylococcus epidermidis and yellow It was confirmed that the difference in cell weight between staphylococci and staphylococci tends to increase. From this, tea seeds that do not substantially contain demon skin are less likely to inhibit the growth of the good bacteria, Staphylococcus epidermidis, and have selective antibacterial properties that inhibit the growth of the bad bacteria, Staphylococcus aureus. I knew there was Comparing Test Groups A and B, it was found that Test Example A, which uses tea seeds that do not substantially contain the skin after oil extraction, has stronger antibacterial properties against Staphylococcus aureus. In addition, even when the water-soluble components were eluted into the aqueous medium from the tea seeds from which the demon skin was removed (test groups A and B), the oil obtained by squeezing the tea seeds from which the demon skin was removed was added. It was found that even in the case (test area C), a bacterial flora-improving agent having appropriate selective antibacterial properties can be obtained.
［第２の試験］
本試験では、鬼皮を実質的に含有しない茶種子の含有量を変化させた複数種類の組成物を作製し、各々の組成物の選択的抗菌性を調べた。
[Second test]
In this test, a plurality of compositions were prepared by varying the content of tea seeds substantially free of demon skin, and the selective antibacterial properties of each composition were investigated.
１．試験区の準備
本試験では、上記第１の試験の試験区Ａと同様の手順に従って、鬼皮を実質的に含有しない茶種子（搾油後）を含有する抗菌組成物を調製した。但し、本試験では、鬼皮を実質的に含有しない茶種子（搾油後）の粉砕物の添加量を０ｗｔ％～５ｗｔ％の範囲で０．５ｗｔ％刻みで異ならせた１１種類の試験用組成物を準備した。
1. Preparation of Test Area In this test, an antibacterial composition containing tea seeds (after oil extraction) substantially free of the skin was prepared according to the same procedure as in Test Area A of the first test. However, in this test, 11 types of test compositions in which the amount of pulverized tea seeds (after oil extraction) that does not substantially contain demon skin was varied in increments of 0.5 wt% in the range of 0 wt% to 5 wt% prepared things.
２．抗菌性評価
ここでは、第１の試験と同様の手順で表皮ブドウ球菌と黄色ブドウ球菌の各々の菌液を準備した。次に、１１種類の抗菌組成物の各々を４ｍｌ分スピッツ管に採集し、各々の菌液を５μｌ添加した。そして、３７℃で２４時間の振盪培養（１５０ｒｐｍ）を実施した後、遠心分離（１００００×ｇ、５分）を行って表皮ブドウ球菌と黄色ブドウ球菌の各々の菌体重量を（ｇ／Ｌ）測定した。結果を図６に示す。
2. Antibacterial Evaluation Here, a bacterial solution of each of Staphylococcus epidermidis and Staphylococcus aureus was prepared in the same procedure as in the first test. Next, 4 ml of each of the 11 kinds of antibacterial compositions was collected in a Spitz tube, and 5 μl of each bacterial solution was added. Then, after performing shaking culture (150 rpm) for 24 hours at 37 ° C., centrifugation (10000 × g, 5 minutes) was performed to measure the cell weight of each of Staphylococcus epidermidis and Staphylococcus aureus (g / L) It was measured. The results are shown in FIG.
３．試験結果
図６に示すように、上述した環境では、鬼皮を実質的に含有しない茶種子（搾油後）の添加量を１．５～３ｗｔ％の範囲内に調節することによって選択的抗菌性が生じることが確認された。このことから、第１の試験の試験区Ｅ（茶種子（鬼皮未除去））と異なり、試験区Ａ（鬼皮を実質的に含有しない茶種子（搾油後））は、適切な選択的抗菌性が発揮されるための含有量に一定の幅があるため、選択的抗菌成分として機能させることが比較的に容易であることが分かった。以上の点から、鬼皮を実質的に含有しない茶種子を選択的抗菌成分として使用することによって、表皮ブドウ球菌優位の細菌叢を形成するための細菌叢改善剤が容易に得られることが分かった。
3. Test results As shown in FIG. 6, in the above-described environment, selective antibacterial properties were obtained by adjusting the amount of tea seeds (after oil extraction) that did not substantially contain demon skin within the range of 1.5 to 3 wt%. was confirmed to occur. From this, unlike test group E (tea seeds (unremoved demon skin)) in the first test, test group A (tea seeds that do not substantially contain demon skin (after oil extraction)) is an appropriate selective It has been found that it is relatively easy to function as a selective antibacterial component because there is a certain range of content for exhibiting antibacterial properties. From the above points, it was found that a bacterial flora improving agent for forming a bacterial flora dominated by Staphylococcus epidermidis can be easily obtained by using tea seeds that do not substantially contain demon skin as a selective antibacterial ingredient. rice field.
以上、ここに開示される技術の具体的な試験例を説明したが、これらは例示にすぎず、請求の範囲を限定するものではない。例えば、第２の試験にて示した含有量は、上述した実験条件に対応した含有量の一例であり、選択的抗菌成分（鬼皮を実質的に含有しない茶種子に由来する成分）の含有量を限定することを意図したものではない。上述した通り、ここに開示される細菌叢改善剤における選択的抗菌成分の適切な含有量は、改善対象物の細菌叢の状態、栄養状態、栄養状態などによって適宜変化し得る。すなわち、請求の範囲に記載の技術には、以上に例示した具体例を様々に変形、変更したものが含まれる。
Specific test examples of the technology disclosed herein have been described above, but these are only examples and do not limit the scope of the claims. For example, the content shown in the second test is an example of the content corresponding to the above-described experimental conditions, and contains a selective antibacterial component (a component derived from tea seeds that does not substantially contain demon skin). It is not intended to be quantitative. As described above, the appropriate content of the selective antibacterial component in the agent for improving the bacterial flora disclosed herein can be appropriately changed depending on the state of the bacterial flora, the nutritional state, the nutritional state, and the like of the subject to be improved. That is, the technology described in the claims includes various modifications and changes of the specific examples illustrated above.
Claims (10)
前記選択的抗菌成分として、前記鬼皮を実質的に含有しない茶種子から抽出した水溶性成分を含有する、細菌叢改善剤。 Derived from tea seeds that do not substantially contain demon skin, it contains a selective antibacterial component that exerts a stronger antibacterial action against Staphylococcus aureus than against Staphylococcus epidermidis,
A bacterial flora-improving agent comprising, as the selective antibacterial component , a water-soluble component extracted from tea seeds substantially free of the demon skin.
前記選択的抗菌成分として、前記鬼皮を実質的に含有しない茶種子から抽出した油分を含有する、細菌叢改善剤。 Derived from tea seeds that do not substantially contain demon skin, it contains a selective antibacterial component that exerts a stronger antibacterial action against Staphylococcus aureus than against Staphylococcus epidermidis,
A bacterial flora-improving agent containing, as the selective antibacterial component , an oil extracted from tea seeds that do not substantially contain the demon skin.
前記選択的抗菌成分として、前記鬼皮を実質的に含有しない茶種子の固形分を含有する、細菌叢改善剤。 Derived from tea seeds that do not substantially contain demon skin, it contains a selective antibacterial component that exerts a stronger antibacterial action against Staphylococcus aureus than against Staphylococcus epidermidis,
A bacterial flora-improving agent containing, as the selective antibacterial component, a solid content of tea seeds substantially free of the demon skin.
前記対象物は、黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）と表皮ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ｅｐｉｄｅｒｍｉｄｉｓ）とを含む細菌叢を有しており、
当該細菌叢における前記黄色ブドウ球菌に対する前記表皮ブドウ球菌の存在比を向上させるように、請求項１～８のいずれか一項に記載の細菌叢改善剤を前記対象物に供給する、細菌叢改善方法。 A bacterial flora improvement method for improving the bacterial flora of a predetermined subject other than humans ,
the object has a flora comprising Staphylococcus aureus and Staphylococcus epidermidis;
Bacterial flora improvement, comprising supplying the bacterial flora improving agent according to any one of claims 1 to 8 to the subject so as to improve the abundance ratio of the Staphylococcus epidermidis to the Staphylococcus aureus in the bacterial flora. Method.
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| JP2020152749A (en) * | 2019-03-18 | 2020-09-24 | 株式会社白形傳四郎商店 | Tea seed oil producing method and shell fruit seed shelling device used for it |
| JP2021046388A (en) * | 2019-09-12 | 2021-03-25 | ピアス株式会社 | Selective antibacterial composition, and external composition for skin |
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