CN112535718A

CN112535718A - Traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress

Info

Publication number: CN112535718A
Application number: CN202011579737.5A
Authority: CN
Inventors: 苗莉; 黄飞; 姜维; 陈永进; 张旻
Original assignee: 7th Medical Center of PLA General Hospital
Current assignee: 7th Medical Center of PLA General Hospital
Priority date: 2020-12-28
Filing date: 2020-12-28
Publication date: 2021-03-23
Anticipated expiration: 2040-12-28
Also published as: CN112535718B

Abstract

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress, which comprises 1-35 parts of turmeric, 1-35 parts of ligusticum wallichii, 1-35 parts of acorus calamus, 1-35 parts of liquorice and 1-500 parts of cocoa butter. The invention has the effects of inducing resuscitation and activating spleen, promoting qi and blood circulation, relaxing tendons and activating collaterals, and is mainly used for treating emotional discomfort, emaciation and dry skin thin and pallid. Can obviously reduce the oxidative damage of muscle mitochondria, clear oxygen free radicals in tissues and inhibit the oxidative apoptosis of the muscle mitochondria; relaxing the blood vessels of muscle tissues, improving the blood supply of the muscle tissues and improving the energy metabolism supply of muscle cells; inhibiting muscle inflammatory reaction, reducing the reduction and atrophy of the whole muscle tissue, and treating the muscle reduction and atrophy caused by psychosomatic stress.

Description

Traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress.

Background

Mental and physical stress refers to a state of stress generated by the human body when the human body is subjected to environmental stimulation beyond the normal adaptive capacity of the human body, and short-term or moderate psychological stress can improve the vigilance level of the human body and is beneficial to the health and functional activities of the human body. However, the long-term psychological stress state or the objective requirement of the environment is far greater than the short-term response ability of the body, and the stress state caused by the adaptation to the strong stimulus causes great burden and damage to the body, even irreversible pathological changes, such as the induction of symptoms such as depression and anxiety, the body is easy to fatigue, thin and atrophied, and the body is easy to cause the sports injury of acute skeletal muscle. On the other hand, the stress reaction of the heart and body often causes depression mood, can reduce the blood pressure, heart rate and blood sugar level, inhibit the whole metabolic rate and energy consumption level of the human body, greatly reduce the blood supply of muscles, aggravate the reduction atrophy of muscle tissues and become the main pathological factors of the clinical stress patients or the depression patients with the persistent wasting and the muscular atrophy.

The process of muscle reduction atrophy is often accompanied by inflammatory reaction of muscle fibers, oxidation vacuole edema appears in muscle mitochondria, the number of ridges in a mitochondrial structure is obviously reduced, the mitochondrial membrane potential is reduced, the ATP level of the muscle is obviously reduced, the energy supply is reduced, the muscle contractility is reduced, and the muscle fiber character transformation of a large number of fast muscles is accompanied, so that the flexibility and the reaction capability of the muscle are reduced. The oxidative damage of muscle mitochondria causes the edema of endoplasmic reticulum, and the levels of various inflammatory factors and apoptosis factors HMGB-1, IL-1 beta, IL-6, TNF-alpha, caspase-3 and the like are obviously increased. Thus, some patients may experience increased apoptosis and inflammatory markers in the muscle or blood, progressive disease progression, significant persistent muscle atrophy, and even life-threatening if not treated effectively in a timely manner.

Modern medicine basically has no specific treatment clinically for the muscular dystrophy caused by psychosomatic stress response, is generally used as an auxiliary reference index for treating depression or anxiety, and has no clinical medicine for direct intervention treatment. Clinical recommendations often suggest amino acid nutritional supplements, which regulate protein metabolism levels only during severe atrophy, by supplementing testosterone or other growth hormones. Because hormone drugs have many adverse reactions, great side effects and small medication safety range and are inconvenient for non-hospitalized patients to use, the key point for solving the problems is to find the drugs or preparations with good safety, exact curative effect and convenient use.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine formula for treating muscle reduction atrophy caused by stress on the heart and body, mainly treats uneasiness, emaciation and dry skin thin and pallid, has the effects of inducing resuscitation and activating the spleen, promoting qi and activating blood, relaxing tendons and activating collaterals, inhibits muscle inflammatory reaction, and reduces the reduction and atrophy of the whole muscle tissue.

In order to achieve the purpose, the invention provides the following technical scheme:

a traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 1-35 parts of turmeric, 1-35 parts of ligusticum wallichii, 1-35 parts of acorus calamus, 1-35 parts of liquorice and 1-500 parts of cocoa butter.

As a further scheme of the invention: the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 5-30 parts of turmeric, 5-30 parts of ligusticum wallichii, 5-30 parts of acorus calamus, 5-30 parts of liquorice and 100-400 parts of cocoa butter.

As a further scheme of the invention: the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 10-20 parts of turmeric, 10-20 parts of ligusticum wallichii, 10-20 parts of acorus calamus, 10-20 parts of liquorice and 1-100 parts of cocoa butter.

As a further scheme of the invention: the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 15 parts of turmeric, 15 parts of ligusticum wallichii, 15 parts of acorus calamus, 15 parts of liquorice and 250 parts of cocoa butter.

The preparation method of the traditional Chinese medicine formula for treating the muscle reduction atrophy caused by the stress of the heart and body comprises the following steps of preparing the raw materials into powder, granules, tablets, capsules, pills, oral solutions, oral suspensions or oral emulsions according to requirements.

The preparation method of the powder comprises the steps of weighing the raw materials in parts by weight at the temperature of below 27 ℃, cleaning and drying the raw materials, grinding the raw materials into powder, and sealing or vacuum preserving the powder.

The preparation method of the granules, the tablets, the capsules, the pills, the oral solution, the oral suspension or the oral emulsion comprises the steps of weighing the turmeric, the ligusticum wallichii, the acorus calamus and the liquorice in parts by weight, cleaning and drying the raw materials in the air, crushing the raw materials and soaking the raw materials in the cocoa butter solvent in parts by weight to obtain a mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain an extracting solution, concentrating the extracting solution, adding a proper amount of common medical auxiliary materials, drying, and respectively preparing into granules, tablets, capsules, pills, oral solutions, oral suspensions or oral emulsions.

The traditional Chinese medicine formula disclosed by the invention has the characteristics that:

turmeric (Curcuma longa L.), also known as: yu jin, Bao Ding Xiang, Mian Ming and Huang Jiang can move qi and break blood stasis, dredge meridians and alleviate pain. It can be used for treating abdominal distention and pain, shoulder pain, pain in arm, heart pain, puerperal blood pain, skin sore, tinea, menoxenia, amenorrhea, and traumatic injury. But also can extract yellow edible dye; the curcumin contained can be used as analytical chemical reagent. The curcumin is acidic polyphenol substance, and the main chain is unsaturated aliphatic and aromatic group. Has effects in inhibiting inflammatory reaction, resisting oxidation, and resisting rheumatoid diseases.

The Ligusticum wallichii (Ligusticum chuanxiong Hort) is distinguished and young in Shanju, and the Ligusticum wallichii is a tip of fragrant fruit, a tip of beard, a tip of the Ligusticum wallichii, a sparrow brain and a Beijing Xiong, and grows in a mild climate environment. Is a traditional Chinese medicine plant which is commonly used for promoting blood circulation and promoting qi circulation, dispelling wind and relieving pain, and the ligusticum wallichii is pungent, warm, fragrant and dry and can disperse and ascend to the vertex; it enters the blood system and goes down to reach the blood sea. Promoting blood circulation and removing blood stasis, and is suitable for various diseases caused by blood stasis; it has good effect in dispelling pathogenic wind and relieving pain, and can be used for treating headache, rheumatalgia, etc.

Acorus calamus L, also known as Acorus calamus, Acorus calamus and Calamus gracilis, has the effects of improving intelligence and chest, hearing and eyesight, removing dampness and detoxifying, and is warm in nature, pungent and bitter in taste. [ FUNCTIONS INDICATION ] has effects of inducing resuscitation, eliminating phlegm, invigorating stomach, and refreshing mind. Can be used for treating epilepsy, phlegm-heat convulsion, chest and abdomen distention, and chronic bronchitis.

Glycyrrhiza uralensis Fisch (Glycyrrhiza uralensis Fisch), which is called radix Glycyrrhizae, radix Glycyrrhiza uralensis, radix Glycyrrhizae Uralensis, Glycyrrhiza uralensis Fisch, Leguminosae, and Glycyrrhiza uralensis Fisch belong to perennial herbs, and are tonifying Chinese herbal medicines. The functions are mainly used for clearing away heat and toxic material, eliminating phlegm and relieving cough, and treating abdominal cavity and the like. It is a dry climate with preference for darkness and dampness, long sunshine and low temperature.

Cocoa butter (cocoa butter), also known as cocoa butter, is a creamy yellow hard natural vegetable fat extracted from cocoa mass, with a very short plasticity range, below 27 ℃ and almost entirely solid (melting at 27.7 ℃). The material will melt rapidly with the increase of temperature, and the material will melt completely when the temperature reaches 35 ℃. Contains abundant polyphenols, has antioxidant effect, and can protect human body against a series of diseases. Contains flavonoid compounds with antioxidant effect, and has effects in preventing angiosclerosis, increasing myocardial activity, and relaxing muscle.

The invention has the effects of inducing resuscitation and activating spleen, promoting qi and activating blood circulation, and relaxing tendons and activating collaterals. The components of the prescription have the following effects in the invention:

the pungent and warm turmeric can disperse warmth and dredge, and has the effects of internally promoting qi and blood, externally dispersing wind-cold, breaking blood and promoting qi circulation, and stimulating the menstrual flow and relieving pain;

the rhizoma ligustici wallichii is a blood-qi-tonifying medicine and has the effects of promoting blood circulation, promoting qi circulation, relieving depression, relieving pain and the like;

the acorus calamus is pungent and fragrant, avoids dirt, can reduce phlegm for resuscitation, remove dampness and invigorate stomach, kill parasites and relieve itching;

the liquorice can tonify the spleen and qi, and can also harmonize the effects of the other medicines to treat various muscle pains;

cocoa butter has effects in moistening dryness, resisting oxidation, improving myocardial activity, and relaxing muscle;

turmeric and rhizoma ligustici wallichii are used as main medicines for stimulating emotion and promoting qi and blood circulation, and acorus calamus is used for matching with liquorice for activating spleen and inducing resuscitation, relaxing tendons and activating collaterals, cocoa butter is used for moistening dryness and relaxing muscles; the prescription has the effects of inducing resuscitation and activating spleen, promoting qi and blood circulation, relaxing tendons and activating collaterals, and is mainly used for treating emotional discomfort, muscle wasting and dry skin thin and pallid.

Compared with the prior art, the invention has the advantages that:

1. the invention can remarkably relax muscle blood vessels, increase the circulating blood flow of muscle endings and increase the blood supply and the nutrition of muscles;

2. the invention can obviously inhibit the level of inflammatory factors of muscle tissues and inhibit inflammatory injury and apoptosis of muscle cells;

3. the invention can obviously inhibit the loss of fast muscle fiber in muscle, inhibit the property transformation of muscle fiber and is beneficial to the maintenance of muscle flexibility;

4. the invention can obviously reduce the oxidation damage of mitochondria in muscle cells, reduce the edema of mitochondria and endoplasmic reticulum, improve the membrane potential of the muscle mitochondria, increase the generation amount of ATP and improve the energy supply of the muscle cells;

5. the invention has the effects of inducing resuscitation and activating spleen, promoting qi and blood circulation, relaxing tendons and activating collaterals, and is mainly used for treating emotional discomfort, emaciation and dry skin thin and pallid. Can obviously reduce the oxidative damage of muscle mitochondria, clear oxygen free radicals in tissues and inhibit the oxidative apoptosis of the muscle mitochondria; relaxing the blood vessels of muscle tissues, improving the blood supply of the muscle tissues and improving the energy metabolism supply of muscle cells; inhibiting muscle inflammatory reaction, reducing the reduction and atrophy of the whole muscle tissue, and treating the muscle reduction and atrophy caused by psychosomatic stress.

Drawings

FIG. 1 is a schematic diagram of the open field behavior distribution of blank control group;

FIG. 2 is a schematic diagram of the open-field behavior distribution of a stress model group;

FIG. 3 is a schematic diagram of the distribution of the field opening behavior of the low-dose group of the traditional Chinese medicine;

FIG. 4 is a schematic diagram of the distribution of the open field behavior of the dose groups in the Chinese medicine;

FIG. 5 is a schematic view of the open field behavior distribution of the high drug dose group;

FIG. 6 is a graphical illustration of the results of a tail suspension struggle test, a mechanical pain threshold test, and a sugar water preference test;

FIG. 7 is a graph of HE staining of the blank control group;

FIG. 8 is a HE staining pattern of stress model group;

FIG. 9 is the HE staining pattern of the low dose group of the Chinese herbs;

FIG. 10 is HE staining pattern of the Chinese medicinal herbs middle dose group;

FIG. 11 is HE staining pattern of high-dose group of Chinese herbs;

FIG. 12 is a photomicrograph of the mitochondrial outer membrane structure of blank control;

FIG. 13 is an electro-microscopic view of the intramitochondrial structure of blank control;

FIG. 14 is an electron microscopic view of the mitochondrial outer membrane structure of stress model group;

FIG. 15 is an electrocomproscopy of intramitochondrial structures from stress model group;

FIG. 16 is an electron microscope image of the mitochondrial outer membrane structure of the low dose group of the Chinese herbal medicines;

FIG. 17 is an electron microscope image of the intramitochondrial structure of the low-dose group of Chinese herbs;

FIG. 18 is an electron microscope image of the mitochondrial outer membrane structure of the dosage group of TCM;

FIG. 19 is an electron microscopic view of the intramitochondrial structure of the dosage group of TCM;

FIG. 20 is an electron microscopic view of the mitochondrial outer membrane structure of the high-dose group of the traditional Chinese medicine,

FIG. 21 is an electron microscopic view of the intramitochondrial structure of the high-dose group of the herb;

FIG. 22 is a schematic view of the results of fluorescent staining detection of the blank control group;

FIG. 23 is a schematic diagram showing the results of fluorescent staining detection of the stress model group;

FIG. 24 is a schematic diagram showing the result of fluorescent staining detection of the low-dose group of traditional Chinese medicines;

FIG. 25 is a schematic diagram of the results of fluorescence staining detection of the dosage groups in the Chinese medicine;

FIG. 26 is a schematic diagram of the fluorescence staining detection results of the high-dose group of the Chinese herbs;

FIG. 27 is a graph showing the results of the Chinese medicine against the muscle inflammatory factor of rats.

Detailed Description

The present invention is further described with reference to the following embodiments, which are only preferred embodiments of the present invention and not intended to limit the present invention in other forms, and any person skilled in the art may change or modify the technical content disclosed above into equivalent embodiments with equivalent changes. Any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the scope of the present invention, unless they depart from the technical spirit of the present invention.

Example 1:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 1 part of turmeric, 1 part of ligusticum wallichii, 1 part of acorus calamus, 1 part of liquorice and 1 part of cocoa butter.

The preparation method comprises weighing the above raw materials at a temperature below 27 deg.C, cleaning, air drying, grinding into powder, and sealing or vacuum preserving.

Example 2:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 35 parts of turmeric, 35 parts of ligusticum wallichii, 35 parts of acorus calamus, 35 parts of liquorice and 500 parts of cocoa butter.

The preparation method comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain extractive solution, concentrating the extractive solution, adding appropriate amount of common medical adjuvants, drying, and making into granule.

Example 3:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 5 parts of turmeric, 5 parts of ligusticum wallichii, 5 parts of acorus calamus, 5 parts of liquorice and 100 parts of cocoa butter.

The preparation method comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain extractive solution, concentrating the extractive solution, adding appropriate amount of common medical adjuvants, drying, and making into tablet.

Example 4:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 30 parts of turmeric, 30 parts of ligusticum wallichii, 30 parts of acorus calamus, 30 parts of liquorice and 400 parts of cocoa butter.

The preparation method of the capsule comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, performing ultrasonic and microwave extraction to obtain an extracting solution, concentrating the extracting solution, adding a proper amount of medical common auxiliary materials, drying, and preparing into capsules.

Example 5:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress is characterized by comprising the following raw materials in parts by weight: 10 parts of turmeric, 10 parts of ligusticum wallichii, 10 parts of acorus calamus, 10 parts of liquorice and 1 part of cocoa butter.

The preparation method comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain extractive solution, concentrating the extractive solution, adding appropriate amount of common medical adjuvants, drying, and making into pill.

Example 6:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress is characterized by comprising the following raw materials in parts by weight: 20 parts of turmeric, 20 parts of ligusticum wallichii, 20 parts of acorus calamus, 20 parts of liquorice and 100 parts of cocoa butter.

The preparation method comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain an extracting solution, concentrating the extracting solution, adding a proper amount of medical common auxiliary materials, drying, and preparing into an oral solution.

Example 7:

the traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress comprises the following raw materials in parts by weight: 15 parts of turmeric, 15 parts of ligusticum wallichii, 15 parts of acorus calamus, 15 parts of liquorice and 250 parts of cocoa butter.

The preparation method comprises weighing Curcuma rhizome, rhizoma Ligustici Chuanxiong, rhizoma Acori Calami, and Glycyrrhrizae radix, cleaning, air drying, pulverizing, and soaking in cacao butter solvent to obtain mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain an extracting solution, concentrating the extracting solution, adding a proper amount of medical common auxiliary materials, drying, and preparing into oral emulsion.

Application experiments:

the traditional Chinese medicine formula is used for muscle atrophy resistance experiments in a chronic stress environment, 50 normal male rats are selected, and the average composition is 5 groups: blank control group, stress model group, low dosage group of traditional Chinese medicine, middle dosage group of traditional Chinese medicine and high dosage group of traditional Chinese medicine; except for the blank control group, all the groups are bound for 12 hours every day, stimulated by noise sound for 2 hours, stimulated by ice water and tail clamping pain for 10 minutes, and the method of reversing day and night lasts for 6 weeks to establish a chronic stress rat model. After maintaining the model stimulation and adjusting for 6 weeks, the Chinese medicinal formula is respectively administered as follows: blank control group (not edible), stress model group (not edible), traditional Chinese medicine low dose group (once a day), traditional Chinese medicine medium dose group (twice a day with 12-hour interval), and traditional Chinese medicine high dose group (three times a day with 8-hour interval); after 7 days of continuous dosing, the behavioral and muscle inflammatory responses of each group of model animals were examined. The behavioral experiments included: open field behavior experiments, mechanical pain threshold test and sugar water preference test.

Open field behavior experiments: in a relatively closed indoor environment, placing an experimental model rat from the middle of an open field, and recording the moving distance (m) and the moving speed (m/s) of the rat in the open field; and the activity time(s) of the central zone. The test time per rat was 15 min.

Hanging tail struggle test: hanging various rats on a cross bar 60cm away from the ground for 6min, wherein the fixing point of the rat is a position slightly 10cm away from the tail, observing and recording the motionless latency of the rat within the first 2min and the total motionless time of the rat within the last 4min, and the motionless state is defined as that the rat stops struggling and the body is in a relaxed state.

Mechanical pain threshold test: under silent environment, the mechanical foot-contracting threshold of each group of rats is measured by a Von-Frey cellosilk method, the strength of the cellosilk is started from 4.0g, the left hind paw pelma of the rat is sequentially stimulated, positive reactions of foot contraction, foot swinging, foot lifting and foot licking of the rat are firstly occurred, and the strength of the cellosilk in the positive reactions is recorded as the pain threshold.

And (4) testing syrup preference: the groups of experimental rats were tested for their preference for a 1% sucrose solution. Adaptive feeding is carried out 3 days before the experiment, rats in each experimental group are fasted and water is forbidden for 24 hours on the 4 th day, then 1% sucrose water and a bottle of normal drinking purified water are placed at the same time, the consumption of the sucrose water and the purified water in 1 hour is recorded, and the proportion of the consumption of the sucrose water to the total consumed liquid is calculated as the preference degree of the sucrose water.

After anesthesia, rats in each group were killed by cervical dislocation, gastrocnemius tissue was taken, HE stained to observe pathological changes, and the mitochondrial state of muscle and fluorescent staining of fast and slow muscle fibers were observed under an electron microscope. The levels of HMGB-1, IL-1 beta, IL-6 and TNF-alpha in the muscle tissues of rats in each group are detected by an Elisa kit method.

And (4) analyzing results:

1. open-field behavior experiments are shown in figures 1-5, wherein figure 1 shows the open-field behavior distribution of the blank control group, and it can be seen that rats move uniformly in the open field and move frequently in the central area;

FIG. 2 shows the open field behavior distribution of the stress model group, showing that the total distance of movement of the rat is small in the open field and the rat has no activity basically in the central area;

the traditional Chinese medicine formula has the advantages that the traditional Chinese medicine low-dose group, the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group are distributed in the open field behavior, the total exercise mileage of a rat in the open field is obviously increased compared with that of a stressed group, and the rat also has obvious activities in a central area.

2. The results of the suspended tail struggle test, the mechanical pain threshold test and the sugar water preference test are shown in fig. 6, and it can be seen that the application of the drug of the invention can improve the struggle time of a stress model, reduce the pain threshold, reduce the pain feeling, significantly improve the sugar water preference of an experimental animal model and improve the stress state of the model.

3. The pathological result and electron microscope detection of the traditional Chinese medicine for resisting rat muscle stress injury are as follows:

the HE staining patterns are shown in FIGS. 7-9. FIG. 7 is a HE staining pattern of a blank control group, FIG. 8 is a HE staining pattern of a stress model group, FIG. 9 is a HE staining pattern of a low dosage group of Chinese medicinal materials, FIG. 10 is a HE staining pattern of a medium dosage group of Chinese medicinal materials, and FIG. 11 is a HE staining pattern of a high dosage group of Chinese medicinal materials;

HE staining chart shows that compared with the blank control group, the stress model has obvious inflammatory reaction in the muscle tissue gaps, the traditional Chinese medicine low-dose group, the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group have obviously reduced inflammatory reaction even have no inflammatory reaction;

as shown in fig. 12-21, mitochondrial electromicroscopic examination. Fig. 12 and 13 are mitochondrial electron microscopic examination images of a blank control group, fig. 14 and 15 are mitochondrial electron microscopic examination images of a stress model group, fig. 16 and 17 are mitochondrial electron microscopic examination images of a low dosage group of a traditional Chinese medicine, fig. 18 and 19 are mitochondrial electron microscopic examination images of a medium dosage group of a traditional Chinese medicine, and fig. 20 and 21 are mitochondrial electron microscopic examination images of a high dosage group of a traditional Chinese medicine;

mitochondrial electron microscopy images show that the structure of the mitochondrial outer membrane of the blank control group is complete, and the structure of the mitochondrial inner ridge is clear; secondly, the outer membrane structure of mitochondria under a mitochondrial electron microscope of the stress model group is partially incomplete, the inner structure of mitochondria basically disappears, and the mitochondria have serious inner air bubbles and oxidative damage; the outer mitochondrial membrane of the electron microscope of the low-dose group, the middle-dose group and the high-dose group of the traditional Chinese medicine has complete structure, the inner mitochondrial structure is partially damaged, and the oxidation of mitochondria is less along with the increase of the administration dose.

4. The results of fluorescence staining detection are shown in FIGS. 22-26. FIG. 22 is a result of fluorescence staining test of blank control group, FIG. 23 is a result of fluorescence staining test of stress model group, FIG. 24 is a result of fluorescence staining test of low dosage group of Chinese medicine, FIG. 25 is a result of fluorescence staining test of medium dosage group of Chinese medicine, and FIG. 26 is a result of fluorescence staining test of high dosage group of Chinese medicine;

the fluorescent staining detection result shows that compared with a blank control group, red fast muscle tissue in a stress model group is seriously lost, and green slow muscle fibers are obviously increased. Compared with stress model group, the traditional Chinese medicine formula of the invention, the traditional Chinese medicine low dose group, the traditional Chinese medicine medium dose group and the traditional Chinese medicine high dose group can obviously reduce muscle loss, and the muscle fiber level of the muscle is improved.

5. As shown in FIG. 27, the results of measuring the levels of HMGB-1, IL-1. beta., IL-6, and TNF-. alpha.in each experimental group: compared with a blank control group, a stress model group, a traditional Chinese medicine low-dose group, a traditional Chinese medicine medium-dose group and a traditional Chinese medicine high-dose group, the levels of HMGB-1, IL-1 beta, IL-6 and TNF-alpha in muscle tissues are remarkably increased. Compared with the stress model group, the traditional Chinese medicine low-dose group, the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group can obviously reduce the levels of HMGB-1, IL-1 beta, IL-6 and TNF-alpha, and the low, medium and high-dose groups have obvious effects; the more significant the effect with increasing dose, the dose-related trend.

Analyzing the map and detecting the conclusion: the traditional Chinese medicine low-dose group, the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group have obvious effect of resisting inflammatory reaction of muscle tissues, can obviously increase the autonomous movement of model animals, reduce tissue pain, inhibit the apoptosis of mitochondria in the muscle tissues of the model animals, inhibit the increase of inflammatory reaction related factors in the muscle tissues, and have more obvious effect of inhibiting the inflammatory reaction along with the increase of the dose and show the dose-related trend.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A traditional Chinese medicine formula for treating muscle reduction atrophy caused by psychosomatic stress is characterized by comprising the following raw materials in parts by weight: 1-35 parts of turmeric, 1-35 parts of ligusticum wallichii, 1-35 parts of acorus calamus, 1-35 parts of liquorice and 1-500 parts of cocoa butter.

2. The traditional Chinese medicine formula for treating the muscle reduction atrophy caused by the psychosomatic stress according to claim 1, is characterized by comprising the following raw materials in parts by weight: 5-30 parts of turmeric, 5-30 parts of ligusticum wallichii, 5-30 parts of acorus calamus, 5-30 parts of liquorice and 100-400 parts of cocoa butter.

3. The traditional Chinese medicine formula for treating the muscle reduction atrophy caused by the psychosomatic stress according to claim 1, is characterized by comprising the following raw materials in parts by weight: 10-20 parts of turmeric, 10-20 parts of ligusticum wallichii, 10-20 parts of acorus calamus, 10-20 parts of liquorice and 1-100 parts of cocoa butter.

4. The traditional Chinese medicine formula for treating the muscle reduction atrophy caused by the psychosomatic stress according to claim 1, is characterized by comprising the following raw materials in parts by weight: 15 parts of turmeric, 15 parts of ligusticum wallichii, 15 parts of acorus calamus, 15 parts of liquorice and 250 parts of cocoa butter.

5. The traditional Chinese medicine composition for treating the muscle atrophy caused by the psychosomatic stress according to any one of claims 1 to 4, wherein the preparation method of the traditional Chinese medicine composition for treating the muscle atrophy caused by the psychosomatic stress comprises the following steps of preparing the raw materials into powder, granules, tablets, capsules, pills, oral solutions, oral suspensions or oral emulsions according to requirements.

6. The traditional Chinese medicine composition for treating the muscle atrophy caused by the stress on the heart and body according to any one of claims 1 to 5, wherein the preparation method of the powder is that the raw materials are weighed according to parts by weight at the temperature of below 27 ℃, are washed and aired, are ground into powder, and are sealed or are preserved in vacuum.

7. The traditional Chinese medicine composition for treating muscle atrophy caused by psychosomatic stress according to any one of claims 1 to 5, wherein the preparation method of the granules, the tablets, the capsules, the pills, the oral solutions, the oral suspensions or the oral emulsions comprises the steps of weighing turmeric, ligusticum wallichii, acorus calamus and liquorice in parts by weight, cleaning, airing, crushing and soaking in cocoa butter solvents in parts by weight to obtain a mixture; decocting the mixture with strong fire for 0.5-1 hour, extracting with ultrasound and microwave to obtain an extracting solution, concentrating the extracting solution, adding a proper amount of common medical auxiliary materials, drying, and respectively preparing into granules, tablets, capsules, pills, oral solutions, oral suspensions or oral emulsions.