CN112457991A - A method for breaking cell wall of Ganoderma spore powder - Google Patents
A method for breaking cell wall of Ganoderma spore powder Download PDFInfo
- Publication number
- CN112457991A CN112457991A CN202011354625.XA CN202011354625A CN112457991A CN 112457991 A CN112457991 A CN 112457991A CN 202011354625 A CN202011354625 A CN 202011354625A CN 112457991 A CN112457991 A CN 112457991A
- Authority
- CN
- China
- Prior art keywords
- spore powder
- ganoderma lucidum
- wall
- breaking
- lucidum spore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for preparing ganoderma lucidum spore powder by breaking the wall, which comprises the steps of carrying out quick-freezing and unfreezing on the ganoderma lucidum spore powder for a plurality of times, and then crushing the ganoderma lucidum spore powder by a roller press. The method adopts multiple quick-freezing and unfreezing operations, and combines high-pressure air to carry out wall breaking operation on the ganoderma lucidum spore powder, can fully play the embrittlement effect of expansion with heat and contraction with cold and the impact effect of high-speed airflow, can carry out efficient wall breaking, has simple control steps and less used equipment, can effectively improve the wall breaking rate of the ganoderma lucidum spore powder, and can carry out large-scale production with low cost.
Description
Technical Field
The invention belongs to the technical field of ganoderma lucidum spore powder processing, and particularly relates to a preparation method for breaking the wall of ganoderma lucidum spore powder.
Background
Ganoderma lucidum, also known as Mesona chinensis Benth, Ganoderma lucidum or Ganoderma lucidum, is a dry fruiting body of Ganoderma lucidum belonging to Polyporaceae of Basidiomycetes, has sweet taste, has effects of strengthening body resistance, consolidating constitution, nourishing and strengthening etc., has high medicinal value, and has wide application in China, Korean peninsula, Japan, southeast Asia and other countries. In the mature period of ganoderma, tiny egg-shaped germ cells, namely seeds of ganoderma, are ejected from the fold of the fungus, and the seeds are called ganoderma spores or ganoderma spore powder. Each ganoderma spore is only 4-6 microns, is a living organism, has a double-wall structure, is surrounded by hard chitin cellulose, and is difficult to be fully absorbed by a human body. After the wall is broken, the medicine is more suitable for direct absorption of intestines and stomach of human body.
In recent years, researchers have conducted a great deal of research on ganoderma lucidum spore powder, and found that ganoderma lucidum spore powder contains various active ingredients, such as polysaccharide, triterpene, amino acid, alkaloid, sterol, inorganic elements and the like, and a series of pharmacological effects of resisting tumor, resisting epilepsy, enhancing immunity, regulating blood sugar and blood fat and the like are confirmed from different levels of genes, proteins or metabolic pathways and the like.
However, ganoderma lucidum spores have two spore walls (polysaccharide walls) composed of chitin and glucan, have concentric layer net structures, are tough and tough, acid and alkali resistant, and are extremely difficult to oxidize and decompose, so that digestion and absorption of effective substances in spores are limited. In order to fully utilize the effective substances in the ganoderma lucidum spores, the wall breaking of the spore powder is required so as to facilitate the utilization of the effective substances by people.
At present, there are several wall-breaking processes, mainly including biological, chemical, physical, mechanical and comprehensive methods. The biological method comprises soaking Ganoderma spore powder with cellulase, hemicellulase, pectinase, etc. to break cell wall. However, this method is time consuming, the enzyme is expensive and is not easily removed after the enzyme is added. At present, the method is used alone less and is combined with other methods for use.
The chemical method is a method for destroying the spore wall structure of Ganoderma by soaking in chemical reagents such as acid and alkali. Because the chemical method has low wall-breaking efficiency, the method is mainly used for wall-breaking pretreatment of ganoderma lucidum spore powder or extraction of effective components of the spore powder and the like.
The physical method is a method for destroying cell wall of Ganoderma spore by using physical effects such as low temperature, freezing (embrittlement), ultrasonic wave, microwave, and supercritical carbon dioxide. The wall breaking efficiency is low by using ultrasonic waves alone, and local high heat is easily generated to denature and inactivate heat-sensitive components in ganoderma lucidum spores such as ganoderma lucidum polypeptides. Microwave also easily causes the heat-sensitive substances in the ganoderma lucidum spores to be inactivated after heating.
The mechanical method refers to a method for destroying the wall of Ganoderma spore by grinding, squeezing, jet-pulverizing, or impacting. The mechanical method is simple and convenient to operate, has high wall breaking rate, can be used for industrial production, and is a ganoderma lucidum spore wall breaking processing method mainly applied to the current industry. However, the single method is not very high in wall breaking effect, so most wall breaking processes combine multiple wall breaking methods together to effectively improve the wall breaking efficiency.
A method for breaking cell wall of Ganoderma spore powder, disclosed in patent application 201910305499.X, comprises mixing Ganoderma spore powder with water, freezing in liquid nitrogen, and dissolving in water bath at 50 deg.C; repeatedly freezing and thawing for several times; removing water, adding carboxylated ferroferric oxide nano magnetic beads, and performing ultrasonic wall breaking to obtain the ganoderma lucidum spore powder after wall breaking. The method comprehensively utilizes physical and mechanical methods to break cell wall.
However, the above method has low wall-breaking efficiency, the wall-breaking rate is only 34-43%, and to increase the wall-breaking rate, the equipment needs to be greatly increased, the cost is increased, and thus the price of the extracted ganoderma lucidum spore powder is high.
Disclosure of Invention
In order to solve the above problems, the primary object of the present invention is to provide a method for breaking the wall of ganoderma lucidum spore powder, which can break the wall of ganoderma lucidum spore powder with low cost and high extraction rate, can effectively improve the wall breaking rate of ganoderma lucidum spore powder, and can perform large-scale production with low cost.
The invention also aims to provide a ganoderma lucidum spore powder wall breaking preparation method, which comprehensively utilizes a physical method and a mechanical method, fully exerts the embrittlement effect of expansion with heat and contraction with cold and the extrusion effect between compression rollers, and effectively breaks the wall, and has the advantages of simple steps, less used equipment and low cost.
In order to achieve the above object, the technical solution of the present invention is as follows.
A method for breaking cell wall of Ganoderma spore powder comprises quickly freezing Ganoderma spore powder for several times, thawing, and pulverizing Ganoderma spore powder by roller press. The wall of the ganoderma lucidum spore powder can be effectively weakened by multiple times of speed and unfreezing, the wall breaking can be carried out by the pressure between the two compression rollers and the speed difference of the two compression rollers, and the wall breaking can be carried out on the ganoderma lucidum spore powder quickly at low cost.
Further, the multiple times are more than 5 times, namely, the ganoderma lucidum spore powder is quickly frozen and then unfrozen, and the circulation is performed for more than 5 times in sequence.
Furthermore, the quick-freezing temperature is-20 ℃, and the quick-freezing time is more than 30 min; the thawing time is 5-20 min. Quick-freezing and then quick-thawing are carried out quickly and continuously, so that the embrittlement degree of the ganoderma lucidum spore powder wall can be effectively improved, and the subsequent wall breaking can be carried out efficiently and quickly.
Further, the linear speed of a front roller of the roller press is 18.65 m/min; the roll speed ratio was 1: 1.27. Through the control of above-mentioned roller speed, can carry out the broken wall effectively.
The invention has the beneficial effects that:
the method adopts multiple quick-freezing and unfreezing operations, and combines high-pressure air to carry out wall breaking operation on the ganoderma lucidum spore powder, can fully play the embrittlement effect of expansion with heat and contraction with cold and the extrusion effect between compression rollers, can carry out efficient wall breaking, has simple control steps and less used equipment, can effectively improve the wall breaking rate of the ganoderma lucidum spore powder, and can carry out large-scale production with low cost.
In addition, other substances are not added, for example, materials such as magnetic beads are not adopted, so that the addition of impurities such as heavy metal is avoided, the purity after wall breaking is higher, and the method is more suitable for subsequent processing.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The method for breaking the wall of the ganoderma lucidum spore powder is realized by two steps:
s1, quick-freezing and unfreezing, namely, firstly, carrying out quick-freezing and unfreezing operation on the ganoderma lucidum spore powder for multiple times;
the multiple times refer to that the quick-freezing and unfreezing steps are fully carried out for at least 5 times, namely, the ganoderma lucidum spore powder is quickly frozen and then unfrozen, and the steps are circulated for more than 5 times in sequence.
In the quick-freezing and unfreezing steps, the quick-freezing temperature is controlled to be-20 ℃, and the quick-freezing time is controlled to be more than 30 min; and the thawing time is 5-20 min. Quick-freezing and then quick-thawing are carried out quickly and continuously, so that the embrittlement degree of the ganoderma lucidum spore powder wall can be effectively improved, and the subsequent wall breaking can be carried out efficiently and quickly.
And S2, extruding, namely inputting the unfrozen ganoderma lucidum spore powder into a compression roller machine, and breaking the wall through the pressure between the two compression rollers and the speed difference between the two compression rollers, so that the ganoderma lucidum spore powder can be quickly broken at low cost. The diameter of the roller pressing machine is 400 mm; the length of the roller is 1000 mm; the linear speed of the front roller is 18.65 m/min; the roll speed ratio was 1: 1.27. Through the control of above-mentioned roller speed, can carry out the broken wall effectively.
In a word, the invention adopts multiple quick-freezing and unfreezing operations, and combines high-pressure air to carry out wall breaking operation on the ganoderma lucidum spore powder, can fully exert the embrittlement effect of expansion with heat and contraction with cold and the extrusion effect between compression rollers, and can carry out high-efficiency wall breaking.
In addition, other substances are not added, for example, materials such as magnetic beads are not adopted for grinding, so that the addition of impurities such as heavy metals is avoided, the subsequent purification process is reduced, the process steps and the flow can be effectively reduced, the wall breaking cost is greatly reduced, the purity of the processed wall-broken ganoderma lucidum spore powder is higher, and the method is more suitable for subsequent processing.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A method for breaking cell wall of Ganoderma spore powder comprises quickly freezing Ganoderma spore powder for several times, thawing, and pulverizing Ganoderma spore powder by roller press.
2. The method for breaking the wall of ganoderma lucidum spore powder according to claim 1, wherein the multiple times are more than 5 times, namely, the ganoderma lucidum spore powder is quickly frozen and then unfrozen, and the steps are sequentially circulated for more than 5 times.
3. The method for breaking the wall of the ganoderma lucidum spore powder as claimed in claim 2, wherein the quick-freezing temperature is 20 ℃ below zero, and the quick-freezing time is more than 30 min; the thawing time is 5-20 min.
4. The method for breaking the wall of ganoderma lucidum spore powder according to claim 1, wherein the linear speed of the front roller of the roller press is 18.65 m/min; the roll speed ratio was 1: 1.27.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011354625.XA CN112457991A (en) | 2020-11-27 | 2020-11-27 | A method for breaking cell wall of Ganoderma spore powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011354625.XA CN112457991A (en) | 2020-11-27 | 2020-11-27 | A method for breaking cell wall of Ganoderma spore powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112457991A true CN112457991A (en) | 2021-03-09 |
Family
ID=74808973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011354625.XA Pending CN112457991A (en) | 2020-11-27 | 2020-11-27 | A method for breaking cell wall of Ganoderma spore powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112457991A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435008A (en) * | 2014-12-08 | 2015-03-25 | 浙江科达生物科技有限公司 | Preparation method of sporoderm-broken lucid ganoderma spore powder |
| CN105796609A (en) * | 2016-03-09 | 2016-07-27 | 孙继勇 | Quenching treatment based ganoderma spore powder wall breaking method |
| CN206474217U (en) * | 2016-08-24 | 2017-09-08 | 锁现民 | The automatic wall-breaking machine of lucidum spore powder |
-
2020
- 2020-11-27 CN CN202011354625.XA patent/CN112457991A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435008A (en) * | 2014-12-08 | 2015-03-25 | 浙江科达生物科技有限公司 | Preparation method of sporoderm-broken lucid ganoderma spore powder |
| CN104435008B (en) * | 2014-12-08 | 2017-11-03 | 浙江科达生物科技有限公司 | The preparation method of ganoderma spove powder |
| CN105796609A (en) * | 2016-03-09 | 2016-07-27 | 孙继勇 | Quenching treatment based ganoderma spore powder wall breaking method |
| CN206474217U (en) * | 2016-08-24 | 2017-09-08 | 锁现民 | The automatic wall-breaking machine of lucidum spore powder |
Non-Patent Citations (1)
| Title |
|---|
| 朱江，等: "灵芝孢子粉的生产工艺研究", 《江西食品工业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Huang et al. | Preparation, deproteinization and comparison of bioactive polysaccharides | |
| CN102379908B (en) | Wall breaking method for fresh and live ganoderma lucidum spores | |
| CN101268815B (en) | Supercritical carbonic anhydride wall-breaking method for melissa powder | |
| CN104774228B (en) | A kind of method that persimmon tannin is prepared from persimmon | |
| CN104042642A (en) | Ganoderma spore wall breaking process | |
| CN108888638A (en) | A kind of processing technology of ganoderma spove powder | |
| CN103044572B (en) | Preparation method of yeast beta-D-glucan | |
| CN106036856B (en) | A kind of highly dissoluble probiotics fermention dendrobe powder and its preparation and application | |
| CN101323649A (en) | Method for extracting mushroom polysaccharide by using ultra-high pressure | |
| CN103059155A (en) | A method of extracting Phellinus igniarius polysaccharides | |
| CN104710541A (en) | Method for preparing laminarin from kelp | |
| CN112999127B (en) | Gentiana scabra bunge compound enzyme and preparation method and application thereof | |
| JP2005027541A5 (en) | Method for producing monosaccharide and / or oligosaccharide | |
| CN101697981B (en) | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore | |
| CN1165032A (en) | Method for breaking cell wall of Ganoderma lucidum spore | |
| CN109198602B (en) | Compound ganoderma lucidum polysaccharide oral liquid and preparation method thereof | |
| CN108034688A (en) | The preparation process of rice bran polysaccharide | |
| CN112457991A (en) | A method for breaking cell wall of Ganoderma spore powder | |
| Dang et al. | Simultaneous treatment of acerola mash by ultrasound and pectinase preparation in acerola juice processing: optimization of the pectinase concentration and pectolytic time by response surface methodology. | |
| CN106395876A (en) | Process for preparing purely-natural nano supermicro calcium powder | |
| CN112442419A (en) | Preparation method of ganoderma lucidum spore oil | |
| CN113577138A (en) | Method for extracting active substances from mulberry leaves | |
| CN106490523A (en) | A kind of radioprotective spiral algae sheet and preparation method thereof | |
| CN108084290A (en) | A kind of method of polysaccharide in microwave radiation exaraction common vetch dish | |
| CN208022937U (en) | A kind of kelp polysaccharide sulfate extraction element |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210309 |
|
| RJ01 | Rejection of invention patent application after publication |