CN106036856B - A kind of highly dissoluble probiotics fermention dendrobe powder and its preparation and application - Google Patents
A kind of highly dissoluble probiotics fermention dendrobe powder and its preparation and application Download PDFInfo
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- CN106036856B CN106036856B CN201610422473.XA CN201610422473A CN106036856B CN 106036856 B CN106036856 B CN 106036856B CN 201610422473 A CN201610422473 A CN 201610422473A CN 106036856 B CN106036856 B CN 106036856B
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- dendrobium nobile
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- 239000006041 probiotic Substances 0.000 title claims abstract description 85
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 85
- 239000000843 powder Substances 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 240000004638 Dendrobium nobile Species 0.000 claims abstract description 146
- 238000000855 fermentation Methods 0.000 claims abstract description 55
- 230000004151 fermentation Effects 0.000 claims abstract description 55
- 108010059892 Cellulase Proteins 0.000 claims abstract description 33
- 229940106157 cellulase Drugs 0.000 claims abstract description 33
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 238000004090 dissolution Methods 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000004108 freeze drying Methods 0.000 claims description 33
- 230000001954 sterilising effect Effects 0.000 claims description 26
- 241000026010 Dendrobium candidum Species 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 16
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 16
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 16
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 15
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 15
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 13
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- 230000000694 effects Effects 0.000 claims description 11
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- 240000001046 Lactobacillus acidophilus Species 0.000 claims 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims 1
- 229910052742 iron Inorganic materials 0.000 claims 1
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- 239000002054 inoculum Substances 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241001076416 Dendrobium tosaense Species 0.000 description 3
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- 241000331528 Chlorophytum capense Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000224466 Giardia Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241001027872 Lactobacillus acidophilus La-14 Species 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of highly dissoluble probiotics fermention dendrobe powder and its preparation and application, enzymolysis processing is carried out to dendrobium nobile using cellulase before fermentation, dendrobium nobile dissolubility and palatability are improved, while the glucide of cellulase hydrolysis generation is but also as the substrate of lactobacillus-fermented；Number of live bacteria of probiotics is up to 10 after fermentation9CFU/g or more, and ferment after dendrobe powder favorable solubility, brew be not easy to be layered, delicate mouthfeel.Disadvantage that is poor, brewing rear easily layering, mouthfeel unpolished rice that the technique improves existing probiotics fermention dendrobe powder dissolubilities.The dissolution mass percent of dendrobe powder significantly improves the dissolubility and palatability of dendrobium nobile up to 94% or more after fermentation.
Description
(1) technical field
The present invention relates to a kind of preparation of biofermentation dendrobe powder, it is related to a kind of highly dissoluble probiotics fermention dendrobe powder
Preparation process and its tunning and application.
(2) background technique
Dendrobium nobile (scientific name: Dendrobium nobile Lindl) also known as lofty or bottomless palpus, bracketplant, Lin Lan, prohibition of life, nobile flower
Deng.Medicinal plant, property slightly sweet flavor is micro- salty, trembles with fear, returns stomach, kidney, lung channel.Reinforcing stomach reg fluid, nourishing Yin and clearing heat.For impairment of yin body fluid deficiency, dry
Polydipsia, deficiency of food are retched, and abnormal heat, mesh are secretly unknown after being ill.Dendrobium candidum in dendrobium nobile ranks first of " nine big mesonas ", with other kinds
Such as Dendrobidium huoshanness, HERBA DENDROBII belong to traditional rare Chinese herbal medicine, in the area such as China and Southeast Asia by favor.It is prebiotic
Bacterium is in human body intestinal canal with anti-mutagenesis, anticancer, the function for improving lactose metabolism rate, reducing serum cholesterol, stimulating immune system
Energy.In addition, the bacteriocin generated is able to suppress the growth for producing malicious Escherichia coli in food and human body, to mitigate or reduce certain
Furthermore the risk poisoned by food a bit also has inhibition Giardiasis (Giardia) and helicobacter pylori (Helicobacter
Pylori effect).
Probiotics has anti-mutagenesis, anticancer in human body intestinal canal, improves lactose metabolism rate, reduces serum cholesterol, stimulation
The function of immune system.In addition, the organic acid generated, bacteriocin are able to suppress the malicious Escherichia coli of production in food and the intracorporal life of people
Furthermore long breeding also has inhibition giardia lamblia (Giardia lamblia to mitigate or reduce the risk of certain things poisonings
) and the effect of helicobacter pylori (Helicobacter pylori) Stile.Lactic acid bacteria is important a kind of probiotics, utilizes it
It ferments to dendrobium nobile, in conjunction with Vacuum Freezing & Drying Technology, activity benefit can be introduced on the basis of reservation dendrobium nobile original health-care efficacy
Raw bacterium improves mouthfeel, the flavor of dendrobium nobile, improves nutritive value and health efficacy, enriches dendrobium nobile product category, make its at
For the ideal ingredient of healthy food.
Dendrobium nobile stem section is rich in cellulose, and lactic acid bacteria does not have the ability of degraded cellulose.Current existing dendrobium nobile lactic acid
Bacterium fermentation technique, is fermented after being only beaten dendrobium nobile with lactic acid bacteria, and content of cellulose is higher in product, has dissolubility
Difference, brews the disadvantages of easy layering at mouthfeel unpolished rice.
There is the technology that dendrobium nobile fermentation is carried out using lactic acid bacteria in existing technology；Have and is added using cellulase progress fruit juice
The report such as work, pollen broken wall.But there has been no the reports processed with cellulase to dendrobium nobile, do not have with cellulase hydrolysis, benefit
Raw bacterium fermentation carries out the report of joint working process to dendrobium nobile.
Dendrobium nobile is as a kind of plant, and containing more fiber, these fibers cannot be degraded by probiotics.Utilize probiotics pair
Dendrobium nobile ferments, although the combination for improving the flavor of dendrobium nobile, realizing probiotics and dendrobium nobile can be played, treated stone
Dry measure used in former times has more cellulose residue to swim in surface layer after brewing, influence product dissolubility and palatability.
Therefore, there are also to be further improved for existing dendrobium nobile probiotics fermention technology.
(3) summary of the invention
It is an object of the present invention to provide a kind of preparation process of highly dissoluble probiotics fermention dendrobe powder and its tunning and
Using, it is intended to by being pre-processed with cellulase to dendrobium nobile before fermentation, solve existing probiotics fermention dendrobe powder dissolution
Property it is poor, brew easily layering, the problems such as mouthfeel is coarse.Mass percent is dissolved after fermentation up to 94% or more, significantly improves stone
Dry measure used in former times dissolubility makes it be not easy to be layered, and improves its palatability.
The technical solution adopted by the present invention is that:
The present invention provides a kind of highly dissoluble probiotics fermention dendrobe powder, and the fermentation dendrobe powder is prepared as follows:
(1) it will be cleaned, be cleaned and comminution pretreatment after the acquisition of fresh dendrobium nobile, and obtain pretreated dendrobium nobile；It (2) again will pretreatment
Dendrobium nobile afterwards mixed with sterile water and be ground into dendrobium nobile homogenate, by dendrobium nobile homogenate sterilizing after cellulase is added, 35~50 DEG C,
Enzyme digestion reaction is carried out under the conditions of 80~150r/min, the dendrobium nobile homogenate after being digested；The cellulase additional amount to locate in advance
Dendrobium nobile quality after reason is calculated as 0.1%~1%；(3) precipitated calcium carbonate is added into the dendrobium nobile homogenate after enzymatic hydrolysis, mixes, inoculation
Probiotics is sealed by fermentation under the conditions of 30~40 DEG C, 100~200r/min after mixing, after fermentation, the stone after being fermented
Dry measure used in former times homogenate；The precipitated calcium carbonate quality dosage is calculated as 5%~20% with pretreated dendrobium nobile quality；The probiotics quality
Dosage is calculated as 5%~20% with pretreated dendrobium nobile quality；(4) trehalose is added into the dendrobium nobile homogenate after fermentation while hot,
Dissolution is mixed, freeze-drying is to mass water content 5% hereinafter, crushing, obtains the highly dissoluble probiotics fermention dendrobe powder；Institute
It states trehalose and is calculated as 5%~15% with dendrobium nobile homogenate quality after fermenting.
Further, step (1) preprocess method are as follows: clean after fresh dendrobium nobile acquisition, clean, be cut into the small of 1~2cm
Section, is crushed to 20 mesh, obtains pretreated dendrobe powder.The dendrobium nobile homogenate preferably uses60Co γ radiation sterilization (dosage
9kGy) carry out sterilization treatment.
Further, step (1) dendrobium nobile is the dendrobium nobile with medical value, including dendrobium candidum, Dendrobidium huoshanness, copper sheet
Dendrobium nobile, purple dendrobium and HERBA DENDROBII.
Further, step (2) the pretreated dendrobium nobile and sterile water mass ratio are 1:10~50.
Further, step (2) cellulase is calculated as 0.4%~1% with dendrobium nobile quality after pre-processing, the cellulose
Enzyme activity is 200U/mg.
Further, step (3) the precipitated calcium carbonate quality dosage is calculated as 5%~7% with pretreated dendrobium nobile quality；
The probiotics quality dosage is calculated as 5%~15% with pretreated dendrobium nobile quality；The trehalose is with the dendrobium nobile after fermenting
Quality is calculated as 5%~10%.
Further, step (3) probiotics is purchased from Du Pont China Holding Co., Ltd. (Shanghai branch company), bacterium powder viable bacteria
Number is 2 × 1010CFU/g, including lactobacillus plantarum Lp-115, Lactobacillus casei Lc-11, lactobacillus paracasei Lpc-37, acidophilus
Lactobacillus La-14 and lactobacillus bulgaricus Lb-64.
Further, step (4) the freeze-drying condition is 5~15Pa of vacuum degree, cold under the conditions of temperature -55~-63 DEG C
It is lyophilized dry to mass water content 5% hereinafter, being crushed to 200 mesh.
Further, the highly dissoluble probiotics fermention dendrobe powder is prepared as follows: (1) being acquired fresh dendrobium nobile
Afterwards, it cleans, clean, be cut into 1~2cm segment, dry, be crushed to 20 mesh, obtain pretreated dendrobium nobile；(2) after pre-processing
Dendrobium nobile mixed with sterile water after cross colloid mill be ground into dendrobium nobile homogenate, use60Co γ radiation sterilization (dosage 9kGy) is to stone
Dry measure used in former times homogenate carries out sterilization treatment, the dendrobium nobile homogenate after being sterilized；Cellulase is added, in 35~50 DEG C, 80~150r/min
Under the conditions of carry out 0.5~1.5h of enzyme digestion reaction, dendrobium nobile after being digested is homogenized；The cellulase is with pretreated dendrobium nobile
Quality is calculated as 0.4%~1%；The pretreated dendrobium nobile and sterile water mass ratio are 1:10~50；(3) to the stone after enzymatic hydrolysis
Precipitated calcium carbonate is added in dry measure used in former times homogenate, mixes, is inoculated with probiotics, it is close under the conditions of 30~40 DEG C, 100~200r/min after mixing
Seal 10~20h of ferment, after fermentation, the dendrobium nobile homogenate after being fermented；The precipitated calcium carbonate quality dosage is to pre-process
Dendrobium nobile quality afterwards is calculated as 5%~7%；The probiotics quality dosage is calculated as 5%~15% with pretreated dendrobium nobile quality；
(4) dissolution is mixed, the tunning added with freeze drying protectant is obtained to the dendrobium nobile homogenate addition trehalose after fermentation while hot,
5~15Pa of vacuum degree, freeze-drying is obtained to mass water content 5% hereinafter, be crushed to 200 mesh under the conditions of temperature -55~-63 DEG C
Obtain the highly dissoluble probiotics fermention dendrobe powder；The trehalose is calculated as 5%~10% with dendrobium nobile homogenate quality after fermenting.
The present invention also provides a kind of highly dissoluble probiotics fermention dendrobe powders in preparing food or health food
Using, the food or health food be by highly dissoluble probiotics fermention dendrobe powder and antierythrite with mass ratio 1:0.01~
0.1 mixes；The antierythrite crushes 200 mesh.
Probiotics used in the present invention is that Du Pont China Holding Co., Ltd. (Shanghai branch company) produces and be commercialized pin
(bacterium powder viable count is 2 × 10 to the bacterium powder sold10CFU/g), have reliable safety, higher lactic acid generation ability and enteron aisle fixed
Plant ability；Dendrobium nobile used is tradition Chinese herbal medicines recorded in pharmacopeia.In fermentation process, probiotics is using contained by dendrobium nobile
Glucide, metabolism, which generates the flavor substances such as lactic acid and other organic acids, had not only improved the flavor of dendrobium nobile, but also inhibited harmful micro-
The breeding of biology, technique and tunning are safe and reliable.
Compared with prior art, beneficial effect of the present invention is mainly reflected in: the present invention provides a kind of highly dissoluble probiotics
Dendrobe powder of fermenting and its preparation and application.Enzymolysis processing is carried out to dendrobium nobile using cellulase before fermentation, improves dendrobium nobile dissolution
Property and palatability, while cellulase hydrolysis generate glucide but also as probiotics fermention substrate；Probiotics after fermentation
Viable count is up to 109CFU/g or more, and ferment after dendrobe powder favorable solubility, brew be not easy to be layered, delicate mouthfeel.It should
Technology improves the disadvantage that existing probiotics fermention dendrobe powder dissolubility is poor, brews rear easily layering, mouthfeel unpolished rice.Treated stone
Dry measure used in former times powder dissolves mass percent up to 94% or more, significantly improves the dissolubility and palatability of dendrobium nobile.
(4) specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
The present invention provides a kind of preparation process of highly dissoluble probiotics fermention dendrobe powder, utilizes cellulase pair before fermenting
Fresh dendrobium nobile carries out enzymolysis processing, dendrobium nobile dissolubility, and the glucide that cellulase hydrolysis generates is improved, but also as prebiotic
The substrate of bacterium fermentation.Probiotics used in the embodiment of the present invention is purchased from Du Pont China Holding Co., Ltd. (Shanghai branch company), bacterium
Powder viable count is 2 × 1010There is CFU/g reliable safety, higher lactic acid to generate ability and intestinal colonisation ability.Processing
Number of live bacteria of probiotics is up to 10 in dendrobe powder afterwards9CFU/g or more dissolves mass percent up to 94% or more.After fermentation
Dendrobe powder favorable solubility, brew be not easy to be layered, delicate mouthfeel, have strong lactic acid flavor, significantly improve the molten of dendrobium nobile
Solution property and palatability.
Embodiment 1
1) it cleans, clean in time after fresh dendrobium candidum acquisition, be cut into 1~2cm segment, be crushed to 20 mesh, obtain pre- place
Dendrobium candidum 100g after reason；
2) pretreated dendrobium candidum according to mass ratio 1:10 ratio be added sterile water, cross colloid mill (JMS-240,
Langfang City Tong Yi Machinery Co., Ltd.) colloidization processing is carried out, dendrobium nobile homogenate is made, uses60Co γ radiation sterilization (dosage
9kGy) dendrobium nobile is homogenized and carries out sterilization treatment, the dendrobium candidum after being sterilized is homogenized 1100g；
3) in terms of pretreated dendrobium nobile quality, into the dendrobium candidum homogenate after sterilizing, by the ratio of mass concentration 0.1%
Example is added 1g cellulase (enzyme activity 200U/mg) and carries out enzyme digestion reaction；
4) hydrolysis temperature is 35 DEG C, revolving speed 80r/min, enzymolysis time 1.5h, the dendrobium candidum homogenate after being digested；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium candidum homogenate after enzymolysis processing, 5g lightweight carbon is added
Sour calcium mixes；
6) in terms of pretreated dendrobium nobile quality, 5g lactobacillus plantarum Lp-115 is accessed in the ratio of quality inoculum concentration 5%,
It mixes；
7) it is sealed by fermentation, 30 DEG C of temperature, speed of agitator 100r/min, fermentation time 20h, obtains tunning 1211g；
8) after fermentation, in terms of total mass of fermentation products, 60.6g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after even dissolution, obtains the tunning added with freeze drying protectant；
9) the tunning thickness 2cm in pallet added with freeze drying protectant is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 10Pa, -55 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobe powder after freeze-drying is crushed to 200 mesh with micronizer, obtains highly dissoluble probiotics fermention
Dendrobe powder 100g；
12) dissolubility is tested: sample to be tested used when test is identical in quality every time, and distilled water volume is also identical.Test
When, sample to be tested is quickly poured into the distilled water of room temperature (25 DEG C), is gently agitated for 2min with glass bar, dissolves it sufficiently；
Then solution 2000r/min is centrifuged 5min；Obtained supernatant is freeze-dried (the same front of condition), make moisture be down to
Sample to be tested is consistent.The solid masses obtained at this time is the sample quality dissolved, is the matter of dissolution with the ratio between sample to be tested
Measure percentage.
13) antierythrite is crushed to 200 mesh；
14) the antierythrite 10g in step 12) is added in step 11) highly dissoluble probiotics fermention dendrobe powder 100g,
Carry out mouthfeel adjustment.Cellulase is not added as control, other operations are identical；After having handled measurement and comparative experiments group with compare
The dissolution mass percent of group judges its dissolubility height.Number of live bacteria of probiotics can in highly dissoluble probiotics fermention dendrobe powder
Up to 1.2 × 109CFU/g, dissolution mass percent is up to 94.3% (it is 62.8% that control sample, which dissolves mass percent).Height dissolution
Property probiotics fermention dendrobium officinale powder favorable solubility, brew be not easy to be layered, delicate mouthfeel, there is strong lactic acid flavor.
Embodiment 2
1) it cleans, clean in time after Dendrobidium huoshanness acquisition, be cut into 1~2cm segment, 20 mesh be crushed to, after being pre-processed
Dendrobium nobile 100g；
2) sterile water is added according to the ratio of mass ratio 1:30 in pretreated Dendrobidium huoshanness, crosses colloid mill and carries out colloidization
It handles (with embodiment 1), dendrobium nobile homogenate is made；Using60Co γ radiation sterilization (dosage 9kGy) sterilizes to dendrobium nobile homogenate
Processing, the dendrobium nobile after being sterilized are homogenized 3100g；
3) in terms of pretreated dendrobium nobile quality, into the dendrobium nobile homogenate after sterilizing, add in the ratio of mass concentration 0.4%
Enter 0.4g cellulase (enzyme activity 200U/mg) and carries out enzyme digestion reaction；
4) hydrolysis temperature is 40 DEG C, revolving speed 100r/min, enzymolysis time 1h, the dendrobium nobile homogenate after being digested；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium nobile homogenate after enzymolysis processing, 7g precipitated calcium carbonate is added,
It mixes；
6) in terms of pretreated dendrobium nobile quality, 10g lactobacillus paracasei is accessed in the ratio of quality inoculum concentration 10%
Lpc-37 is mixed；
7) it is sealed by fermentation, 35 DEG C of temperature, speed of agitator 150r/min, fermentation time 16h, obtains tunning 3117.4g；
8) after fermentation, in terms of total mass of fermentation products, 311.7g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after even dissolution, obtains the tunning added with freeze drying protectant；
9) the tunning thickness 1cm in pallet added with freeze drying protectant is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 10Pa, -60 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobium nobile after freeze-drying is crushed to 200 mesh with micronizer, obtains highly dissoluble probiotics fermention stone
Dry measure used in former times powder 220g；
12) antierythrite is crushed to 200 mesh；
13) the antierythrite 22g in step 12) is added in step 11) highly dissoluble probiotics fermention dendrobe powder 220g,
Carry out mouthfeel adjustment.
Cellulase is not added as control, other operations are identical；Measurement and comparative experiments group and control group after having handled
Mass percent is dissolved, judges its dissolubility height.Dissolubility test: method is same as Example 1.Highly dissoluble probiotics hair
Number of live bacteria of probiotics is up to 2.7 × 10 in ferment dendrobe powder9CFU/g, dissolution mass percent is up to 96.8%, control sample solubility
Measuring percentage is 61.4%.Highly dissoluble probiotics fermention Dendrobidium huoshanness powder favorable solubility, brew be not easy to be layered, mouthfeel it is thin
It is greasy, there is strong lactic acid flavor.
Embodiment 3
1) it cleans in time after HERBA DENDROBII acquisition, cleans, is cut into 1~2cm segment, 20 mesh are crushed to, after being pre-processed
Dendrobium nobile 100g；
2) sterile water is added into pretreated HERBA DENDROBII according to the ratio of mass ratio 1:50, crosses colloid mill and carries out glue
Bodyization handles (with embodiment 1), and dendrobium nobile homogenate is made；Using60Co γ radiation sterilization (dosage 9kGy) is homogenized dendrobium nobile and carries out
Sterilization treatment, the HERBA DENDROBII after being sterilized are homogenized 5110g；
3) in terms of pretreated dendrobium nobile quality, in mass concentration 1% ratio to after sterilizing dendrobium nobile homogenate in 1g is added
Cellulase (enzyme activity 200U/mg) carries out enzyme digestion reaction；
4) hydrolysis temperature is 50 DEG C, revolving speed 150r/min, enzymolysis time 0.5h；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium nobile homogenate after enzymolysis processing, 5g precipitated calcium carbonate is added,
It mixes；
6) 15g lactobacillus acidophilus La-14 is accessed, is mixed；
7) it is sealed by fermentation, 40 DEG C of temperature, speed of agitator 200r/min, fermentation time 20h, obtains tunning 5131g；
8) after fermentation, in terms of total mass of fermentation products, 500g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after dissolution, obtains the tunning added with freeze drying protectant；
9) the tunning thickness 2cm in pallet added with freeze drying protectant is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 15Pa, -55 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobe powder after freeze-drying is crushed to 200 mesh with micronizer, obtains highly dissoluble probiotics fermention
Dendrobe powder 310g；
12) antierythrite is crushed to 200 mesh；
13) antierythrite in step 12) is added in step 11) highly dissoluble probiotics fermention dendrobe powder 310g
3.1g carries out mouthfeel adjustment.
Cellulase is not added as control, other operations are identical；Measurement and comparative experiments group and control group after having handled
Mass percent is dissolved, judges its dissolubility height.Dissolubility test: method is same as Example 1.Highly dissoluble probiotics hair
After ferment in dry measure used in former times powder number of live bacteria of probiotics up to 1.5 × 109CFU/g, dissolution mass percent is up to 94.1%, control sample solubility
Measuring percentage is 64.2%.HERBA DENDROBII powder favorable solubility after fermentation, brew be not easy to be layered, delicate mouthfeel, have strong
Lactic acid flavor.
Embodiment 4
1) it cleans, clean in time after purple dendrobium acquisition, be cut into 1~2cm segment, 20 mesh be crushed to, after being pre-processed
Dendrobium nobile 100g；
2) sterile water is added according to the ratio of mass ratio 1:30 in pretreated purple dendrobium, crosses colloid mill and carries out colloidization
It handles (with embodiment 1), dendrobium nobile homogenate is made；Using60Co γ radiation sterilization (dosage 9kGy) sterilizes to dendrobium nobile homogenate
Processing, the dendrobium nobile after being sterilized are homogenized 3100g；
3) in terms of pretreated dendrobium nobile quality, into the dendrobium nobile homogenate after sterilizing, add in the ratio of mass concentration 0.4%
Enter 0.4g cellulase (enzyme activity 200U/mg) and carries out enzyme digestion reaction；
4) hydrolysis temperature is 40 DEG C, revolving speed 100r/min, enzymolysis time 1h, the dendrobium nobile homogenate after being digested；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium nobile homogenate after enzymolysis processing, 7g precipitated calcium carbonate is added,
It mixes；
6) 10g lactobacillus paracasei Lpc-37 is accessed, is mixed；
7) it is sealed by fermentation, 35 DEG C of temperature, speed of agitator 150r/min, fermentation time 16h, obtains tunning 3117g；
8) after fermentation, in terms of total mass of fermentation products, 312g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after dissolution, obtains the tunning added with freeze drying protectant；
9) the tunning thickness 1cm in pallet added with freeze drying protectant is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 10Pa, -60 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobium nobile after freeze-drying is crushed to 200 mesh with micronizer, obtains highly dissoluble probiotics fermention stone
Dry measure used in former times powder 180g；
12) antierythrite is crushed to 200 mesh；
13) the antierythrite 4g in step 12) is added in step 11) highly dissoluble probiotics fermention dendrobe powder 180g,
Carry out mouthfeel adjustment.
Cellulase is not added as control, other operations are identical；Measurement and comparative experiments group and control group after having handled
Mass percent is dissolved, judges its dissolubility height.Dissolubility test: method is same as Example 1.Highly dissoluble probiotics hair
Number of live bacteria of probiotics is up to 2.7 × 10 in ferment dendrobe powder9CFU/g, dissolution mass percent is up to 94.5%, control sample solubility
Measuring percentage is 65.7%.Purple dendrobium powder favorable solubility after fermentation, brew be not easy to be layered, delicate mouthfeel, have strong
Lactic acid flavor.
Comparative example
For the superiority for further illustrating highly dissoluble probiotics fermention dendrobium nobile powder preparation technique of the present invention, tie below
The comparative example that enzymatic hydrolysis and fermentation condition except the present invention are implemented is closed to be illustrated.
Using by except invention enzymatic hydrolysis and fermentation condition prepare probiotics fermention dendrobe powder, compared to the present invention for its
Dissolubility wants significant lower, and dissolution mass percent is average by low 17% than the present invention；And number of live bacteria of probiotics wants low two
It is more than the order of magnitude, only 107CFU/g.Comparative example 1
1) it cleans, clean in time after fresh dendrobium candidum acquisition, be cut into 1~2cm segment, be crushed to 20 mesh, obtain pre- place
Dendrobium candidum 100g after reason；
2) purified water after sterilizing is added according to the ratio of mass ratio 1:10 in pretreated dendrobium candidum, crosses colloid mill
(JMS-240, Langfang City Tong Yi Machinery Co., Ltd.) carries out colloidization processing, and dendrobium nobile homogenate is made, uses60Co γ radiation sterilization
Method (dosage 9kGy) is homogenized dendrobium nobile and carries out sterilization treatment, and the dendrobium candidum after being sterilized is homogenized 1100g；
3) in terms of pretreated dendrobium nobile quality, into the dendrobium candidum homogenate after sterilizing, by mass concentration 0.05%
Ratio is added 0.5g cellulase (enzyme activity 200U/mg) and carries out enzyme digestion reaction；
4) hydrolysis temperature is 32 DEG C, revolving speed 60r/min, enzymolysis time 2h, the dendrobium candidum homogenate after being digested；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium candidum homogenate after enzymolysis processing, 3g lightweight carbon is added
Sour calcium mixes；
6) in terms of pretreated dendrobium nobile quality, 3g lactobacillus plantarum Lp-115 is accessed in the ratio of quality inoculum concentration 3%,
It mixes；
7) it is sealed by fermentation, 28 DEG C of temperature, speed of agitator 80r/min, fermentation time 80h, obtains tunning 1106.5g；
8) after fermentation, in terms of total mass of fermentation products, 40g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after dissolution, obtains the tunning added with freeze drying protectant；
9) the tunning thickness 2cm in pallet added with freeze drying protectant is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 10Pa, -55 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobe powder after freeze-drying is crushed to 200 mesh, acquired probiotics fermention dendrobe powder with micronizer
74g；
12) antierythrite is crushed to 200 mesh；
13) the antierythrite 7.4g in step 12) is added in step 11) probiotics fermention dendrobe powder 74g, carries out mouthfeel
Adjustment.
Cellulase is not added as control, other operations are identical；Measurement and comparative experiments group and control group after having handled
Mass percent is dissolved, judges its dissolubility height.Dissolubility test: method is same as Example 1.Probiotics fermention dendrobe powder
Middle number of live bacteria of probiotics is 1.3 × 107CFU/g, dissolves mass percent number 77.3%, and control sample dissolution mass percent is
65.4%.Before probiotics fermention dendrobium officinale powder dissolubility, the mouthfeel prepared under this condition is better than processing, but than the present invention
Under the conditions of product it is poor；There is certain lactic acid flavor, but when product of the number of live bacteria of probiotics under the conditions of the present invention in product
Low two orders of magnitude.
Comparative example 2
1) it cleans, clean in time after fresh dendrobium candidum acquisition, be cut into 1~2cm segment, be crushed to 20 mesh, obtain pre- place
Dendrobium candidum 100g after reason；
2) purified water after sterilizing is added according to the ratio of mass ratio 1:10 in pretreated dendrobium candidum, crosses colloid mill
(JMS-240, Langfang City Tong Yi Machinery Co., Ltd.) carries out colloidization processing, and dendrobium nobile homogenate is made, uses60Co γ radiation sterilization
Method (dosage 9kGy) is homogenized dendrobium nobile and carries out sterilization treatment, and the dendrobium candidum after being sterilized is homogenized 1100g；
3) in terms of pretreated dendrobium nobile quality, into the dendrobium candidum homogenate after sterilizing, by the ratio of mass concentration 1.5%
Example is added 1.5g cellulase (enzyme activity 200U/mg) and carries out enzyme digestion reaction；
4) hydrolysis temperature is 55 DEG C, revolving speed 180r/min, enzymolysis time 20min, the dendrobium candidum homogenate after being digested；
5) in terms of pretreated dendrobium nobile quality, in the dendrobium candidum homogenate after enzymolysis processing, 25g lightweight carbon is added
Sour calcium mixes；
6) in terms of pretreated dendrobium nobile quality, 25g lactobacillus plantarum Lp- is accessed in the ratio of quality inoculum concentration 25%
115, it mixes；
7) it is sealed by fermentation, temperature 45 C, speed of agitator 220r/min, fermentation time 22h, obtains tunning 1151.5g；
8) after fermentation, in terms of total mass of fermentation products, 240g trehalose is added while hot as freeze drying protectant, is mixed
Tank is put after dissolution, obtains the tunning for adding freeze drying protectant；
9) the tunning thickness 2cm for adding freeze drying protectant in pallet is lyophilized, freezes rapidly in quick freezing repository；
10) it is freeze-dried under the conditions of vacuum degree 10Pa, -55 DEG C of temperature, moisture is made to be down to 4.5%；
11) dendrobe powder after freeze-drying is crushed to 200 mesh with micronizer, obtains probiotics fermention dendrobe powder
180g；
12) antierythrite is crushed to 200 mesh；
13) the antierythrite 18g in step 12) is added in step 11) probiotics fermention dendrobe powder 180g, carries out mouthfeel
Adjustment.
Cellulase is not added as control, other operations are identical；Measurement and comparative experiments group and control group after having handled
Mass percent is dissolved, judges its dissolubility height.Dissolubility test: method is same as Example 1.Probiotics fermention dendrobe powder
Middle number of live bacteria of probiotics is 2.25 × 106CFU/g, for dissolution mass percent up to 74.2%, control sample dissolves mass percent
It is 63.6%.Before probiotics fermention dendrobium officinale powder dissolubility, the mouthfeel prepared under this condition is better than processing, but than this hair
Product under the conditions of bright is poor；There is certain lactic acid flavor, but number of live bacteria of probiotics is than the product under the conditions of the present invention in product
Want low three orders of magnitude.
Claims (10)
1. a kind of highly dissoluble probiotics fermention dendrobe powder, it is characterised in that the fermentation dendrobe powder is prepared as follows: (1)
It will be cleaned, be cleaned and comminution pretreatment after the acquisition of fresh dendrobium nobile, and obtain pretreated dendrobium nobile；(2) after pre-processing again
Dendrobium nobile mixed with sterile water and be ground into dendrobium nobile homogenate, by dendrobium nobile homogenate sterilizing after cellulase is added, in 35~50 DEG C, 80
Enzyme digestion reaction is carried out under the conditions of~150r/min, the dendrobium nobile homogenate after being digested；The cellulase additional amount is to pre-process
Dendrobium nobile quality is calculated as 0.1%~1% afterwards；(3) precipitated calcium carbonate is added into the dendrobium nobile homogenate after enzymatic hydrolysis, mixes, is inoculated with prebiotic
Bacterium is sealed by fermentation under the conditions of 30~40 DEG C, 100~200r/min after mixing, and after fermentation, the dendrobium nobile after being fermented is even
Slurry；The precipitated calcium carbonate quality dosage is calculated as 5%~20% with pretreated dendrobium nobile quality；The probiotics quality dosage
5%~20% is calculated as with pretreated dendrobium nobile quality；(4) trehalose is added into the dendrobium nobile homogenate after fermentation, mixes dissolution,
Freeze-drying is to mass water content 5% hereinafter, crushing, obtains the highly dissoluble probiotics fermention dendrobe powder；The trehalose
5%~20% is calculated as with the dendrobium nobile homogenate quality after fermenting.
2. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that step (1) described preprocess method
Are as follows: it cleans, clean in time after fresh dendrobium nobile acquisition, be cut into 1~2cm segment, be crushed to 20 mesh, obtain pretreated dendrobium nobile；
Step (2) pretreated dendrobium nobile and sterile water mass ratio are 1:10~50.
3. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that step (1) described dendrobium nobile includes iron
Skin dendrobium nobile, Dendrobidium huoshanness, Dendrobium Moniliforme, purple dendrobium and HERBA DENDROBII.
4. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that step (2) cellulase with
Pretreated dendrobium nobile quality is calculated as 0.4%~1%, and the cellulase activity is 200U/mg.
5. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that the precipitated calcium carbonate quality is used
Amount is calculated as 5%~7% with pretreated dendrobium nobile quality；The probiotics quality dosage is calculated as with pretreated dendrobium nobile quality
5%~15%；The trehalose is calculated as 5%~10% with the dendrobium nobile quality after fermenting.
6. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that step (3) described probiotics includes
Lactobacillus plantarum, Lactobacillus casei, lactobacillus paracasei, lactobacillus acidophilus and lactobacillus bulgaricus.
7. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that step (4) the freeze-drying item
Part is 5~15Pa of vacuum degree, and freeze-drying is to mass water content 5% hereinafter, being crushed to 200 under the conditions of temperature -55~-63 DEG C
Mesh.
8. highly dissoluble probiotics fermention dendrobe powder as described in claim 1, it is characterised in that the fermentation dendrobe powder is by as follows
Method preparation: (1) it will fresh dendrobium nobile acquisition after in time cleans, clean, be cut into 1~2cm segment, be crushed to 20 mesh, obtain pre- place
Dendrobium nobile after reason；(2) ratio of 1:10~50 in mass ratio again, pretreated dendrobium nobile is mixed with sterile water, then crosses glue
Body barreling wears into dendrobium nobile homogenate, uses60Co γ radiation sterilization is homogenized dendrobium nobile and carries out sterilization treatment, the dendrobium nobile after being sterilized
Homogenate；Cellulase is added, 0.5~1.5h of enzyme digestion reaction is carried out under the conditions of 35~50 DEG C, 80~150r/min, is digested
Dendrobium nobile homogenate afterwards；The cellulase additional amount is calculated as 0.4%~1% with dendrobium nobile quality after pre-processing；(3) to after enzymatic hydrolysis
Precipitated calcium carbonate is added in dendrobium nobile homogenate, mixes, probiotics is inoculated with, after mixing under the conditions of 30~40 DEG C, 100~200r/min
It is sealed by fermentation 10~20h, after fermentation, the dendrobium nobile homogenate after being fermented；The precipitated calcium carbonate quality dosage to locate in advance
Dendrobium nobile quality is calculated as 5%~10% after reason；The probiotics quality dosage is calculated as 5%~15% with dendrobium nobile quality after pre-processing；
(4) trehalose is added into the dendrobium nobile homogenate after fermentation, mixes dissolution, the tunning added with freeze drying protectant is obtained, true
5~15Pa of reciprocal of duty cycle, freeze-drying is obtained to mass water content 5% hereinafter, be crushed to 200 mesh under the conditions of temperature -55~-63 DEG C
The highly dissoluble probiotics fermention dendrobe powder；The trehalose is calculated as 5%~10% with dendrobium nobile homogenate quality after fermenting.
9. highly dissoluble probiotics fermention dendrobe powder described in a kind of claim 1 is preparing the application in food or health food.
10. application as claimed in claim 9, it is characterised in that the food or health food are to send out highly dissoluble probiotics
Ferment dendrobe powder and antierythrite are mixed with mass ratio 1:0.01~0.1.
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| CN109793856B (en) * | 2019-03-28 | 2021-06-08 | 宁波工程学院奉化研究院 | Preparation method of dendrobium officinale powder |
| EP4070819A4 (en) | 2019-12-06 | 2023-01-25 | Nii, Keni | Composition having wholesome personalized intestinal flora diversity function and application |
| TWI737159B (en) * | 2020-02-07 | 2021-08-21 | 蕾迪詩生物科技股份有限公司 | Preparation method of Dendrobium spp. anti-oxidant extract |
| CN111387494A (en) * | 2020-04-15 | 2020-07-10 | 安徽牧龙山铁皮石斛生物科技开发有限公司 | Preparation method of special dendrobium officinale superfine powder for meal replacement powder |
| CN112155206B (en) * | 2020-10-10 | 2023-08-29 | 龙岩市农业科学研究所 | Application of dendrobium candidum in puffed food, puffed food and processing method |
| KR102362106B1 (en) * | 2021-03-19 | 2022-02-14 | 주식회사 현대바이오랜드 | Method for preparing fermented dendrobium candidum flower and cosmetic composition comprising the fermented dendrobium candidum flower |
| CN113575813A (en) * | 2021-08-05 | 2021-11-02 | 贵州启明农业科技发展有限责任公司 | Preparation method of dendrobium officinale and brown rice fermented beverage |
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