CN104940644A - Method for fermenting dendrobium officinale employing lactic acid bacteria, as well as fermented product and application of dendrobium officinale - Google Patents
Method for fermenting dendrobium officinale employing lactic acid bacteria, as well as fermented product and application of dendrobium officinale Download PDFInfo
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Abstract
The invention discloses a method for fermenting dendrobium officinale employing lactic acid bacteria, as well as a fermented product and application of the dendrobium officinale. The method comprises the following steps: squeezing fresh dendrobium officinale strips into juice, or carrying out freeze drying and superfine grinding; fermenting by employing lactic acid bacteria as a dendrobium officinale fermenting strain; and finishing fermentation, adding mycose to a fermented product as a protective agent, and carrying out freeze drying, so as to obtain the fermented product with activated probiotics. The dendrobium officinale is fermented with the lactic acid bacteria; the activated probiotics are introduced on the basis of the original health efficacy of the dendrobium officinale; the mouthfeel and flavor of the dendrobium officinale are improved; the nutrient value and health efficacy of the dendrobium officinale are improved; the variety of dendrobium officinale series products is enriched; the content of the probiotics of the fermented dendrobium officinale is not less than 109.
Description
Technical field
The present invention relates to technical field of microbial fermentation, particularly relate to a kind of method for fermenting lactic acid bacteria of Herba Dendrobii and tunning thereof and application.
Background technology
Herba Dendrobii (Dendrobii candidum) is grown nonparasitically upon another plant for the orchid family is perennial herbaceous plant, and be listed in " first of China's nine large Herba mesonae chinensis ", being listed in top grade Chinese medicine with Ganoderma, Radix Ginseng and Cordyceps etc., is traditional rare Chinese medicine." Chinese Pharmacopoeia " records the effect specifically having reinforcing stomach reg fluid, nourishing YIN and clearing away heat.The secretion of this external enhancing human body immunity power, resisting fatigue, promotion gastric juice is aid digestion, also there is unique effects hepatoprotective function of gallbladder promoting, the enhancing aspect such as blood sugar lowering, antitumor.
Probiotic bacteria have in human body intestinal canal antimutagenic, anticancer, improve lactose metabolism rate, reduce serum cholesterol, stimulating immune system function.In addition, the bacteriocin produced can suppress to produce the growth of malicious escherichia coli in food and human body, thus reduce or reduce the risk of some alimentary toxicosis, also there is the effect of suppression giardia lamblia (Giardia lamblia Stile) and helicobacter pylori (Helicobacter pylori) in addition.Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus casei (Lactobacillus casei) and Lactobacillus paracasei (Lactobacillus paracasei) are important probiotic bacterias, it is utilized to carry out fermentation process to Herba Dendrobii, active probiotic can be introduced on the basis of the original health-care effect of Herba Dendrobii, improve the mouthfeel of Herba Dendrobii, local flavor, improve its nutritive value and health-care effect, enrich Herba Dendrobii series of products category, become the high-quality batching of functional food.
At present, the Herba Dendrobii product on market has and belongs to traditional type, as Herba Dendrobii, or simple composite type, as dissolved granule, capsule etc., kind is comparatively single, scientific and technological content is lower.
Therefore, prior art need development.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of method for fermenting lactic acid bacteria of Herba Dendrobii and tunning thereof and application, be intended to solve existing Herba Dendrobii product variety few, the problems such as effect is single.
Technical scheme of the present invention is as follows:
A method for fermenting lactic acid bacteria for Herba Dendrobii, wherein, comprises the following steps:
1) the fresh bar of Herba Dendrobii is squeezed the juice, or after lyophilization micronizing;
2) lactobacillus is adopted to be that Herba Dendrobii fermented bacterium ferments;
3), after fermentation ends, it is protective agent that tunning adds trehalose, carries out lyophilization, must have the tunning of active probiotic.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, step 1) in, the step of being squeezed the juice by fresh for Herba Dendrobii bar is as follows:
Remove the horny layer of epidermis of the fresh bar of Herba Dendrobii, clean and dry;
Fresh for Herba Dendrobii bar is cut into 2 ~ 5cm segment, prepares purified water by weight 1:3 ~ 1:8;
Herba Dendrobii stem section is dropped into juice extractor, adds equivalent purified water and squeeze the juice;
Be that medium carries out vacuum filtration with 100 object nylon gauzes, filtering residue residue purified water washs 2 times, and filtrate collection is for subsequent use, obtains the fresh bar juice of Herba Dendrobii.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, when adopting the fresh bar juice of Herba Dendrobii to ferment, fermentation step is: in the ratio of inoculum concentration 5% ~ 12%, lactobacillus is accessed the fresh bar juice of Herba Dendrobii, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, step 1) in, by as follows for the step of described dendrobium candidum ultrafine powder:
Remove the horny layer of epidermis of the fresh bar of Herba Dendrobii, clean and dry, fresh for Herba Dendrobii bar is cut into 2 ~ 5cm segment;
Lyophilization under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, makes moisture drop to less than 5%;
Super micron mill is utilized to be crushed to 200 orders.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, when adopting dendrobium candidum ultrafine powder to ferment, fermentation step is: regulate dendrobium candidum ultrafine powder water content to be 60% ~ 90%, pH nature with sterilized water; In the ratio of inoculum concentration 5% ~ 12%, strain is accessed dendrobium candidum ultrafine powder suspension, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, step 3) in, described cryodesiccated detailed process is: the trehalose adding 5% ~ 10% at tunning is protective agent, and mixing is dissolved; Under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, carry out lyophilization, make moisture drop to less than 6%.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, wherein, step 2) in, described lactobacillus comprises Lactobacillus plantarum, lactobacillus casei and Lactobacillus paracasei.
A kind of Herba Dendrobii tunning, wherein, adopts the method for fermenting lactic acid bacteria of Herba Dendrobii as above to prepare.
An application for Herba Dendrobii tunning as above, wherein, is used for the preparation of health product, food by described Herba Dendrobii tunning.
Beneficial effect: the invention provides a kind of method for fermenting lactic acid bacteria of Herba Dendrobii and tunning thereof and application, lactobacillus is utilized to ferment to Herba Dendrobii, active probiotic is introduced on the basis of the original health-care effect of Herba Dendrobii, improve the mouthfeel of Herba Dendrobii, local flavor, improve its nutritive value and health-care effect, enriched Herba Dendrobii series of products category, the Herba Dendrobii probiotic bacteria content after fermentation can reach 10
9above.
Detailed description of the invention
The invention provides a kind of method for fermenting lactic acid bacteria of Herba Dendrobii and tunning thereof and application, for making object of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The method for fermenting lactic acid bacteria of Herba Dendrobii provided by the present invention, adopts probiotic bacteria to carry out fermentation process to it, has both remained its original health-care effect, in turn introduced the beneficial effect of probiotic bacteria.The present invention's Lactobacillus plantarum, lactobacillus casei and Lactobacillus paracasei used is that Du Pont China Holding Co., Ltd. (Shanghai branch company) produces and carried out the mycopowder of commercialization sale, has reliable safety, stronger lactic acid produces ability and intestinal colonisation ability; Herba Dendrobii used originates from Yunnan, best in quality, pure natural, pollution-free.
The method for fermenting lactic acid bacteria of described Herba Dendrobii, comprises the following steps:
1) the fresh bar of Herba Dendrobii is squeezed the juice, or after lyophilization micronizing;
2) lactobacillus is adopted to be that Herba Dendrobii fermented bacterium ferments;
3), after fermentation ends, it is protective agent that tunning adds trehalose, carries out lyophilization, must have the tunning of active probiotic.
Particularly, step 1) in, the step of being squeezed the juice by fresh for Herba Dendrobii bar is as follows:
A) the fresh bar of the Herba Dendrobii after gathering, removes horny layer of epidermis with clean toothbrush, cleans and dries;
B) be cut into 0.5 ~ 2cm segment with shears, and weighed, prepared purified water by weight 1:3 ~ 1:8 simultaneously;
C) Herba Dendrobii stem section is dropped into juice extractor, add equivalent purified water and squeeze the juice;
D) be that medium carries out vacuum filtration with 100 object nylon gauzes, filtering residue residue purified water washs 2 times, and filtrate collection is for subsequent use, obtains the fresh bar juice of Herba Dendrobii.
By as follows for the step of described dendrobium candidum ultrafine powder:
A) the fresh bar of the Herba Dendrobii after gathering, removes horny layer of epidermis with clean toothbrush, cleans and dries, be cut into 2 ~ 5cm segment with shears;
B) lyophilization under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, makes moisture drop to less than 5%;
C) then utilize super micron mill to be crushed to 200 orders, to obtain final product.
Step 2) in, described lactobacillus comprises Lactobacillus plantarum, lactobacillus casei and Lactobacillus paracasei.
When adopting the fresh bar juice of Herba Dendrobii to ferment, fermentation step is: in the ratio of inoculum concentration 5% ~ 12%, lactobacillus is accessed the fresh bar juice of Herba Dendrobii, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
When adopting dendrobium candidum ultrafine powder to ferment, fermentation step is: regulate dendrobium candidum ultrafine powder water content to be 60% ~ 90%, pH nature with sterilized water; In the ratio of inoculum concentration 5% ~ 12%, strain is accessed dendrobium candidum ultrafine powder suspension, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
Step 3) in, described cryodesiccated detailed process is: the trehalose adding 5% ~ 10% at tunning is protective agent, and mixing is dissolved; Under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, carry out lyophilization, make moisture drop to less than 6%.
Also provide a kind of tunning of Herba Dendrobii in the present invention, described tunning adopts the method for fermenting lactic acid bacteria of Herba Dendrobii as above to prepare.The application of described tunning is also provided in the present invention, can by described tunning for the preparation of health product, food.
Below by way of specific embodiment, the present invention will be further described.
Embodiment 1
1. horny layer of epidermis removed by the clean toothbrush of the fresh bar of Herba Dendrobii, cleans and dries; Be cut into 0.5 ~ 2cm segment with shears, and weighed, prepared purified water by weight 1:8 simultaneously; Herba Dendrobii stem section is dropped into juice extractor, adds equivalent purified water and squeeze the juice; Be that medium carries out vacuum filtration with 100 object nylon gauzes, filtering residue residue purified water washs 2 times, and filtrate collection is for subsequent use.
2., in the ratio of inoculum concentration 5%, strain is accessed the fresh juice of Herba Dendrobii, in 32 DEG C, under 100r/min condition, sealing and fermenting 20h.
3. after fermentation ends, the trehalose that tunning adds 8% is protective agent, and mixing is dissolved; Lyophilization under vacuum 12Pa, temperature ~ 63 DEG C condition, makes moisture drop to less than 6%, must be rich in the Herba Dendrobii tunning of active probiotic.
Embodiment 2:
1. horny layer of epidermis removed by the clean toothbrush of the fresh bar of Herba Dendrobii, cleans and dries; Be cut into 0.5 ~ 2cm segment with shears, and weighed, prepared purified water by weight 1:5 simultaneously; Herba Dendrobii stem section is dropped into juice extractor, adds equivalent purified water and squeeze the juice; Be that medium carries out vacuum filtration with 100 object nylon gauzes, filtering residue residue purified water washs 2 times, and filtrate collection is for subsequent use.
2., in the ratio of inoculum concentration 8%, strain is accessed the fresh juice of Herba Dendrobii, in 35 DEG C, under 150r/min condition, sealing and fermenting 16h.
3. after fermentation ends, the trehalose that tunning adds 10% is protective agent, and mixing is dissolved; Lyophilization under vacuum 15Pa, temperature-60 DEG C of conditions, makes moisture drop to less than 6%, must be rich in the Herba Dendrobii tunning of active probiotic.
Embodiment 3:
1. the fresh bar of Herba Dendrobii, removes horny layer of epidermis with clean toothbrush, cleans and dries, be cut into 2 ~ 5cm segment with shears; Lyophilization under vacuum 8Pa, temperature-63 DEG C of conditions, makes moisture drop to less than 5%; Super micron mill is utilized to be crushed to 200 orders.
2. regulate dendrobium candidum ultrafine powder water content to be 70%, pH nature with sterilized water; In the ratio of inoculum concentration 6%, by probiotic bacteria strain access dendrobium candidum ultrafine powder suspension, in temperature 32 DEG C of condition bottom fermentation 20h.
3. after fermentation ends, the trehalose that tunning adds 5% is protective agent, and mixing is dissolved; Lyophilization under vacuum 8Pa, temperature-63 DEG C of conditions, makes moisture drop to less than 6%, must be rich in the Herba Dendrobii tunning of active probiotic.
Embodiment 4:
1. the fresh bar of Herba Dendrobii, removes horny layer of epidermis with clean toothbrush, cleans and dries, be cut into 2 ~ 5cm segment with shears; Lyophilization under vacuum 14Pa, temperature-60 DEG C of conditions, makes moisture drop to less than 5%; Super micron mill is utilized to be crushed to 200 orders.
2. regulate dendrobium candidum ultrafine powder water content to be 80%, pH nature with sterilized water; In the ratio of inoculum concentration 6%, by probiotic bacteria strain access dendrobium candidum ultrafine powder suspension, in 35 DEG C, 150r/min bottom fermentation 18h.
3. after fermentation ends, the trehalose that tunning adds 10% is protective agent, and mixing is dissolved; Lyophilization under vacuum 12Pa, temperature-60 DEG C of conditions, makes moisture drop to less than 6%, must be rich in the Herba Dendrobii tunning of active probiotic.
Lactobacillus is utilized to ferment to Herba Dendrobii, active probiotic is introduced on the basis of the original health-care effect of Herba Dendrobii, improve the mouthfeel of Herba Dendrobii, local flavor, improve its nutritive value and health-care effect, enriched Herba Dendrobii series of products category, the Herba Dendrobii probiotic bacteria content after fermentation can reach 10
9above.
Should be understood that, application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (9)
1. a method for fermenting lactic acid bacteria for Herba Dendrobii, is characterized in that, comprises the following steps:
1) the fresh bar of Herba Dendrobii is squeezed the juice, or after lyophilization micronizing;
2) lactobacillus is adopted to be that Herba Dendrobii fermented bacterium ferments;
3), after fermentation ends, it is protective agent that tunning adds trehalose, carries out lyophilization, must have the tunning of active probiotic.
2. the method for fermenting lactic acid bacteria of Herba Dendrobii according to claim 1, is characterized in that, in step 1), the step of being squeezed the juice by fresh for Herba Dendrobii bar is as follows:
Remove the horny layer of epidermis of the fresh bar of Herba Dendrobii, clean and dry;
Fresh for Herba Dendrobii bar is cut into 2 ~ 5cm segment, prepares purified water by weight 1:3 ~ 1:8;
Herba Dendrobii stem section is dropped into juice extractor, adds equivalent purified water and squeeze the juice;
Be that medium carries out vacuum filtration with 100 object nylon gauzes, filtering residue residue purified water washs 2 times, and filtrate collection is for subsequent use, obtains the fresh bar juice of Herba Dendrobii.
3. the method for fermenting lactic acid bacteria of Herba Dendrobii according to claim 1, it is characterized in that, when adopting the fresh bar juice of Herba Dendrobii to ferment, fermentation step is: in the ratio of inoculum concentration 5% ~ 12%, lactobacillus is accessed the fresh bar juice of Herba Dendrobii, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
4. the method for fermenting lactic acid bacteria of Herba Dendrobii according to claim 1, is characterized in that, in step 1), by as follows for the step of described dendrobium candidum ultrafine powder:
Remove the horny layer of epidermis of the fresh bar of Herba Dendrobii, clean and dry, fresh for Herba Dendrobii bar is cut into 2 ~ 5cm segment;
Lyophilization under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, makes moisture drop to less than 5%;
Super micron mill is utilized to be crushed to 200 orders.
5. the method for fermenting lactic acid bacteria of Herba Dendrobii according to claim 1, is characterized in that, when adopting dendrobium candidum ultrafine powder to ferment, fermentation step is: regulate dendrobium candidum ultrafine powder water content to be 60% ~ 90%, pH nature with sterilized water; In the ratio of inoculum concentration 5% ~ 12%, strain is accessed dendrobium candidum ultrafine powder suspension, in 30 ~ 40 DEG C, 100 ~ 150r/min condition bottom fermentation, 10 ~ 20h.
6. the method for fermenting lactic acid bacteria of Herba Dendrobii according to claim 1, is characterized in that, in step 3), described cryodesiccated detailed process is: the trehalose adding 5% ~ 10% at tunning is protective agent, and mixing is dissolved; Under vacuum 5 ~ 15Pa, temperature-55 ~-63 DEG C of conditions, carry out lyophilization, make moisture drop to less than 6%.
7., according to the method for fermenting lactic acid bacteria of the arbitrary described Herba Dendrobii of claim 1 ~ 6, it is characterized in that, step 2) in, described lactobacillus comprises Lactobacillus plantarum, lactobacillus casei and Lactobacillus paracasei.
8. a Herba Dendrobii tunning, is characterized in that, adopt as arbitrary in claim 1 ~ 7 as described in the method for fermenting lactic acid bacteria of Herba Dendrobii prepare.
9. an application for Herba Dendrobii tunning as claimed in claim 8, is characterized in that, described Herba Dendrobii tunning is used for the preparation of health product, food.
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| CN105534876A (en) * | 2016-01-21 | 2016-05-04 | 北京工商大学 | Dendrobe fermentation raw stock as well as preparation method and application thereof |
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| CN106070609A (en) * | 2016-06-02 | 2016-11-09 | 谭恺 | A kind of preparation method and application of Herba Dendrobii Yoghourt |
| CN106360683A (en) * | 2016-08-29 | 2017-02-01 | 广西壮族自治区农业科学院农产品加工研究所 | Dendrobium officinale and banana compound probiotic fermentation products and method thereof |
| CN106616974A (en) * | 2016-11-14 | 2017-05-10 | 霍山三宝生物保健品有限公司 | Production method for dendrobe enzyme capable of promoting immunity |
| CN106722928A (en) * | 2016-11-14 | 2017-05-31 | 霍山三宝生物保健品有限公司 | A kind of stem of noble dendrobium ferment production method with along intestines bowel relaxing functions |
| CN106962478A (en) * | 2017-05-05 | 2017-07-21 | 广州赛莱拉干细胞科技股份有限公司 | A kind of store method of beneficial bacteria of intestinal tract |
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| CN110214929A (en) * | 2019-06-14 | 2019-09-10 | 华南农业大学 | A kind of dendrobium candidum leaf fermentation sauce and preparation method thereof |
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| CN115364025A (en) * | 2022-08-26 | 2022-11-22 | 珠海市丝域生物科技有限公司 | Composition with scalp care effect and preparation method and application thereof |
| CN117883353A (en) * | 2024-01-18 | 2024-04-16 | 万京(广东)生物技术有限公司 | Preparation and application of dendrobium nobile fermentation liquor |
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| CN105534876A (en) * | 2016-01-21 | 2016-05-04 | 北京工商大学 | Dendrobe fermentation raw stock as well as preparation method and application thereof |
| CN105707628B (en) * | 2016-03-11 | 2019-03-08 | 三株福尔制药有限公司 | A kind of composition and preparation method thereof of probiotics fermention dendrobium candidum protocorm |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
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| CN106360683A (en) * | 2016-08-29 | 2017-02-01 | 广西壮族自治区农业科学院农产品加工研究所 | Dendrobium officinale and banana compound probiotic fermentation products and method thereof |
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| CN108308468A (en) * | 2018-02-02 | 2018-07-24 | 植然方适（杭州）健康科技有限公司 | A kind of dendrobium candidum enzyme beverage and preparation method thereof with defaecation, stomach-nourishing function |
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| CN110623180A (en) * | 2019-09-12 | 2019-12-31 | 湖南农业大学 | Dendrobium officinale fermented probiotic beverage and preparation method thereof |
| CN110742909A (en) * | 2019-10-29 | 2020-02-04 | 安徽省霍山县益寿堂中药材开发有限公司 | Method for carrying out biological fermentation by utilizing fresh traditional Chinese medicinal materials |
| CN112167629A (en) * | 2020-10-22 | 2021-01-05 | 厦门鹰君药业有限公司 | Dendrobium officinale powder and preparation method thereof |
| CN113576991A (en) * | 2021-08-06 | 2021-11-02 | 广东药科大学 | Preparation method of dendrobium officinale fermentation liquor |
| CN115364025A (en) * | 2022-08-26 | 2022-11-22 | 珠海市丝域生物科技有限公司 | Composition with scalp care effect and preparation method and application thereof |
| CN117883353A (en) * | 2024-01-18 | 2024-04-16 | 万京(广东)生物技术有限公司 | Preparation and application of dendrobium nobile fermentation liquor |
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Application publication date: 20150930 |