CN113577138A - Method for extracting active substances from mulberry leaves - Google Patents
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Abstract
The invention relates to the technical field of extraction of bioactive substances, in particular to a method for extracting active substances from mulberry leaves, which comprises the steps of firstly carrying out ultrasonic treatment on mulberry leaf ultrafine powder and cellulase, and jointly realizing cell rupture by an enzymatic method and a mechanical crushing method; then, the dissolved substances are subjected to repeated deposition operation by adopting temperature alternation for many times, and are determined by ninhydrin, so that the removal rate of the protein in the mulberry leaf extracting solution is improved by a great temperature difference; and finally, the surfactant is adopted to assist the water extraction operation, so that the separation of the target components is realized, and the purity of the target components is improved. On the basis of effectively removing protein, the invention simultaneously realizes the extraction of mulberry leaf polysaccharide, alkaloid and mulberry leaf flavone in mulberry leaves and researches the synergistic effect of the three.
Description
Technical Field
The invention relates to the technical field of extraction of bioactive substances, in particular to a method for extracting active substances from mulberry leaves.
Background
China is the biggest mulberry planting country in the world, and mulberry leaves are traditional Chinese medicines in China and are leaves of mulberry (Morusalbal.) belonging to Morus of Moraceae; because of the effects of dispelling wind and dissipating heat, moistening dryness and clearing away the lung-heat, and clearing liver and improving vision, the traditional Chinese medicine composition is widely used for treating wind-heat type common cold, dizziness and headache, lung heat type dry cough, conjunctival congestion and dim flower; the mulberry leaves contain various functional components, such as mulberry leaf flavone, polyphenol, mulberry leaf polysaccharide, alkaloid, phytosterol, amino acid and other unknown active substances with medical and health care effects on human bodies.
Chen Jian nationality et al indicate: the mulberry leaf polysaccharide has the functions of regulating glycometabolism, reducing blood sugar and improving diabetes symptoms; researches show that the main component of alkaloid in mulberry leaves is 1-deoxynojirimycin which has the obvious function of reducing blood sugar; the flavonoid and the alkaloid are combined to more effectively inhibit the rise of blood sugar, which shows that the flavonoid and the mulberry leaf alkaloid have a certain synergistic effect; the prior art also finds that the mulberry leaf polysaccharide and the alkaloid have a certain synergistic compatibility; therefore, it is very meaningful to obtain the extracts of the mulberry leaf polysaccharide, the alkaloid and the mulberry leaf flavone and research the synergistic hypoglycemic effect of the three.
The mulberry leaf has high content of leaf protein, and is also one of Chinese plants with high content of leaf protein, research shows that the content of the protein in the mulberry leaf is up to more than 20%, and the amino acid of the protein is close to lean pork, chicken and the like, but the protein has no practical significance for the effect of reducing blood sugar, and after the content of the leaf protein in the extract is increased, the mixture of the protein and active ingredients influences the concentration of the active ingredients, so that the effect of the active ingredients in a certain amount of the extract is reduced, and therefore, an extraction and separation method which has sufficient protein removal and contains main ingredients of mulberry leaf polysaccharide, alkaloid and mulberry leaf flavone is needed to obtain the mixed extract of the mulberry leaf polysaccharide, the alkaloid and the mulberry leaf flavone.
And the extraction process in the prior art mainly aims at the extraction of single components and researches the action of the single components, and on the basis of better synergistic effect disclosed in the prior art, the extraction of the single components not only causes the waste of beneficial components, but also increases the operation procedures and cost.
Disclosure of Invention
Aiming at the technical defects, the invention aims to provide a method for extracting active substances from mulberry leaves, and the principle of the invention is as follows: on the basis of effectively removing protein, the extraction of mulberry leaf polysaccharide, alkaloid and mulberry leaf flavone in mulberry leaves is realized at the same time, and the application of the mulberry leaf polysaccharide, alkaloid and mulberry leaf flavone in blood sugar reduction is researched through the synergistic effect of the three.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 3.5-6.5, and performing ultrasonic treatment to obtain eluate;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.c for 8-12min, freezing at-15 deg.c to-5 deg.c for 1-2 hr, and centrifuging at room temperature to obtain supernatant;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, adding an ethanol solution of a mulberry leaf flavone extraction surfactant, an ethanol solution of a mulberry leaf polysaccharide extraction surfactant and an ethanol solution of a mulberry leaf alkaloid extraction surfactant, performing ultrasonic treatment, extracting, and evaporating to dryness to obtain a mulberry leaf active substance.
Preferably, the solution with pH of 3.5-6.5 in the step (1) is diluted hydrochloric acid or diluted sulfuric acid solution, and the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 15-20mL:1 g.
Preferably, the ultrasonic treatment conditions of the step (1) are as follows: performing ultrasonic treatment at room temperature under the conditions of 150 and 200W for 40-60 min.
Preferably, the mass ratio of the mulberry leaf ultrafine powder in the step (1) to the cellulase is 100: 2-5.
Preferably, the number of times of the temperature alternation of the step (2) for the deposition operation is determined by ninhydrin until ninhydrin detection in the lower clear liquid is negative.
Preferably, in the step (3), the surfactant in the ethanol solution of the mulberry leaf flavone extraction surfactant is triton X-100, and the mass concentration of the ethanol solution of the mulberry leaf flavone extraction surfactant is 3-5%.
Preferably, in the step (3), the surfactant in the ethanol solution of the mulberry leaf polysaccharide extraction surfactant is one of sodium dodecyl sulfate, cetyl trimethyl ammonium bromide and octyl phenol polyoxyethylene ether-10, and the mass concentration of the ethanol solution of the mulberry leaf polysaccharide extraction surfactant is 1-3%.
Preferably, in the step (3), the surfactant in the ethanol solution of the mulberry leaf alkaloid extraction surfactant is one of tween 80, silk plate 80 and APG0801, and the mass concentration of the ethanol solution of the mulberry leaf alkaloid extraction surfactant is 2-4%.
Preferably, the ultrasonic treatment conditions in the step (3) are as follows: performing ultrasonic treatment at 25 + -5 deg.C under 80-120W for 10-25 min.
Preferably, in the step (3), the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extraction surfactant, the ethanol solution of the mulberry leaf polysaccharide extraction surfactant and the ethanol solution of the mulberry leaf flavone extraction surfactant to the supernatant is 0.5-0.8: 1, the volume fraction of the ethanol solution is 50-70%.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention firstly adopts mulberry leaf ultra-fine powder and cellulose for ultrasonic treatment, the grain diameter of the mulberry leaf ultra-fine powder is between 10 and 25 mu m, and the cell rupture is realized by an enzyme method and a mechanical crushing method; and then, an ultrasonic extraction technology is adopted, so that the extraction solution and the material generate a cavitation effect mutually, bubbles in the extraction solution are formed, enlarged and compressed, the collision probability between the solid sample and the extraction solution is increased, and the mass transfer efficiency of the extract transferred from the material to the extraction solution is improved.
2. The invention adopts repeated deposition operation of temperature alternation for many times for the dissolved substance, and is determined by ninhydrin, under the heating operation, the natural structure of protein molecules is destroyed, the hydrophobic effect among the protein molecules is improved, then the mutual aggregation of the protein molecules is realized, after the repeated deposition of temperature alternation, the huge temperature difference is realized, and then the removal rate of the protein in the mulberry leaf extracting solution is improved;
3. the invention adopts surfactant to assist water extraction operation, and the surfactant adopts mulberry leaf flavone extraction surfactant, mulberry leaf polysaccharide extraction surfactant and alkaloid extraction surfactant, and the purpose of target extraction is realized through the three surfactants.
4. The invention adopts surfactant to assist water extraction operation, the surfactant is a substance which has strong surface activity and can obviously reduce the surface tension of the liquid, the molecules are amphiphilic molecules formed by two different particles, one side is hydrophilic group, and the other side is hydrophobic group; the principle of adopting the surfactant for auxiliary extraction is as follows: the power of the surfactant adsorbed on the interface is to reduce the free energy of the phase interface, the wetting and solubilizing actions of the surfactant can accelerate the leaching of effective components, and meanwhile, water is used as a solvent, so that the technical defects of cost, difficulty in separation and poor safety caused by the use of an organic solvent are reduced; the main function of the method is also to separate target components and improve the purity of target components; the surfactant is safe and environment-friendly, is easy to treat, and is a green auxiliary extraction technology.
Drawings
FIG. 1 is a graph showing the relationship between the concentration of an active substance produced in example 2 of the present invention and the inhibition rate of α -glucosidase activity.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. The experimental methods described in the examples of the present invention are all conventional methods unless otherwise specified.
Example 1
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 3.5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 150W for 60min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 15mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 2;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 12min, freezing at-5 deg.C for 2 hr, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 4 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, then adding an ethanol solution of triton X-100, an ethanol solution of sodium dodecyl sulfate and an ethanol solution of a silk disc 80, carrying out ultrasonic treatment for 10min at 20 ℃ under the condition of 120W, then extracting and evaporating to obtain a mulberry leaf active substance;
wherein the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extraction surfactant, the ethanol solution of the mulberry leaf polysaccharide extraction surfactant and the ethanol solution of the mulberry leaf flavone extraction surfactant to the lower clear liquid is 0.5: 1, the volume fraction of the ethanol solution is 70%.
Example 2
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 180W for 50min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 17mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 3;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 10min, freezing at-10 deg.C for 1.5h, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 3 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, then adding a triton X-100 ethanol solution, an octyl phenol polyoxyethylene ether-10 ethanol solution and an APG0801 ethanol solution, carrying out ultrasonic treatment for 15min at 30 ℃ under the condition of 100W, and then extracting and evaporating to obtain a mulberry leaf active substance;
wherein the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extracted surfactant, the ethanol solution of the mulberry leaf polysaccharide extracted surfactant and the ethanol solution of the mulberry leaf flavone extracted surfactant to the lower clear liquid is 0.6: 1, the volume fraction of the ethanol solution is 60 percent.
Example 3
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 6.5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 200W for 40min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 20mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 5;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 8min, freezing at-15 deg.C for 1h, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 4 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, then adding an ethanol solution of triton X-100, an ethanol solution of hexadecyl trimethyl ammonium bromide and an ethanol solution of Tween 80, carrying out ultrasonic treatment for 25min at 30 ℃ under the condition of 80W, and then extracting and evaporating to obtain a mulberry leaf active substance;
wherein the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extraction surfactant, the ethanol solution of the mulberry leaf polysaccharide extraction surfactant and the ethanol solution of the mulberry leaf flavone extraction surfactant to the lower clear liquid is 0.8: 1, the volume fraction of the ethanol solution is 50%.
Comparative example 1
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 180W for 50min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 17mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 3;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 10min, freezing at-10 deg.C for 1.5h, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 3 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, adding a triton X-100 ethanol solution, performing ultrasonic treatment at 30 ℃ under the condition of 100W for 15min, extracting, and evaporating to obtain a mulberry leaf active substance;
wherein the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extracted surfactant, the ethanol solution of the mulberry leaf polysaccharide extracted surfactant and the ethanol solution of the mulberry leaf flavone extracted surfactant to the lower clear liquid is 0.6: 1, the volume fraction of the ethanol solution is 60 percent.
Comparative example 2
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 180W for 50min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 17mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 3;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 10min, freezing at-10 deg.C for 1.5h, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 3 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, then adding an octyl phenol polyoxyethylene ether-10 ethanol solution, carrying out ultrasonic treatment for 15min at 30 ℃ under the condition of 100W, then extracting and evaporating to obtain a mulberry leaf active substance;
wherein the mass ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extracted surfactant, the ethanol solution of the mulberry leaf polysaccharide extracted surfactant and the ethanol solution of the mulberry leaf flavone extracted surfactant to the lower clear liquid is 0.6: 1, the volume fraction of the ethanol solution is 60 percent.
Comparative example 3
A method for extracting active substances from folium Mori comprises the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 5, performing ultrasonic treatment, and performing ultrasonic treatment at room temperature under 180W for 50min to obtain eluate;
wherein the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 17mL:1 g; the mass ratio of the mulberry leaf ultrafine powder to the cellulase is 100: 3;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.C for 10min, freezing at-10 deg.C for 1.5h, and centrifuging at room temperature after the deposition operation is finished to obtain supernatant;
the number of times of alternately repeating the deposition operation at the temperature is determined by ninhydrin, and is specifically operated for 3 times, and ninhydrin in the lower clear liquid is detected to be negative;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, adding APG0801 ethanol solution, performing ultrasonic treatment at 30 ℃ for 15min under the condition of 100W, extracting, and evaporating to obtain folium mori active substances;
wherein the mass ratio of the total amount of the surfactant ethanol solution to the lower clear liquid is 0.6: 1, the volume fraction of the ethanol solution is 60 percent.
The content of the active substances of mulberry leaf polysaccharide, mulberry leaf flavone and alkaloid in the examples 1-3 is tested and compared with the comparative examples 1-3, and the specific results are shown in the following table 1:
table 1 results of content test of active materials obtained in examples 1 to 3 and comparative examples 1 to 3
| Item | Mulberry leaf polysaccharide (mg/g) | Mulberry leaf flavone (mg/g) | Alkaloid (mg/g) | Protein removal Rate (%) |
| Example 1 | 91.169 | 158.429 | 1.574 | 92.3 |
| Example 2 | 96.627 | 164.577 | 1.644 | 96.7 |
| Example 3 | 93.554 | 150.216 | 1.543 | 93.8 |
| Comparative example 1 | / | 166.413 | / | 96.3 |
| Comparative example 2 | 98.526 | / | / | 96.8 |
| Comparative example 3 | / | / | 1.785 | 96.5 |
"/" indicates no data;
as seen from Table 1, the mulberry leaves of examples 1-3 have high contents of polysaccharides, flavones and alkaloids of mulberry leaves as active substances; and compared with the comparative example, although the content is slightly less than that of the comparative example, the content is not much different from that of the comparative example, and the mutual influence between the synergistic extractions can be considered to be not large.
The following test method in the prior art is adopted to study the good inhibition effect of active substances in mulberry leaves on alpha-glucosidase, and the specific test method is shown in table 2:
alpha-glucosidase inhibition rate/% [1- (A)2-A1)/A0]×100；
Adding 0.1mL of alpha-glucosidase solution with concentration of 0.2U/mL into 2mL and 0.1mol/L phosphate buffer solution (pH 6.8), performing water bath at 37 ℃ for 15min, reacting the sample solutions of examples 1-3 and comparative examples 1-3 for 10min, adding 0.25mL and 25mmol/L substrate PNPG, performing water bath for 30min, adding 0.1mol/L and 2mLNa2CO3The reaction was terminated and the absorbance (A) at 400nm was measured2) (ii) a The absorbance value was measured by replacing the sample solution with 1mL of buffer solution0) (ii) a The absorbance value is measured by replacing enzyme solution with 0.1mL buffer solution1). The assay was repeated 3 times:
TABLE 2 inhibition results of α -glucosidase by active materials prepared in examples 1 to 3 and comparative examples 1 to 3
| Item | Inhibition rate of alpha-glucosidase |
| Example 1 | 95％ |
| Example 2 | 96％ |
| Example 3 | 93％ |
| Comparative example 1 | 37％ |
| Comparative example 2 | 32％ |
| Comparative example 3 | 40％ |
The results show that after the synergistic effect of the three components, the effect is obviously better than that of the single component, the invention also combines the products of the comparative example 1 and the comparative example 2, the products of the comparative example 2 and the comparative example 3 are combined, and the comparative example 3 and the comparative example I are combined to carry out research, the combination of the products of the comparative example 1 and the comparative example 2 has the inhibition rate of 73 percent to alpha-glucosidase, the combination of the products of the comparative example 2 and the comparative example 3 has the inhibition rate of 78 percent to alpha-glucosidase, the combination of the products of the comparative example 3 and the comparative example I has the inhibition rate of 81 percent to alpha-glucosidase, which are all obviously lower than that of the embodiment, and the results show that the inhibition effect of the three components on alpha-glucosidase after the combined effect is more excellent.
The relationship between the concentration of the active substance prepared in example 2 and the inhibition rate of the alpha-glucosidase activity is shown in fig. 1, and the results show that the inhibition rate of the alpha-glucosidase activity reaches 85% when the concentration of the active substance is 1mg/mL, and both table 2 and fig. 1 show that the hypoglycemic effect of the present application is excellent.
It will be apparent to those skilled in the art that various changes and modifications may be made in the invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. A method for extracting active substances from mulberry leaves is characterized by comprising the following steps:
(1) adding folium Mori micropowder and cellulase into solution with pH of 3.5-6.5, and performing ultrasonic treatment to obtain eluate;
(2) carrying out multiple temperature alternate repeated deposition operations on the dissolved matter obtained in the step (1), wherein the temperature alternate repeated deposition method comprises the following steps: heating at 100 deg.c for 8-12min, freezing at-15 deg.c to-5 deg.c for 1-2 hr, and centrifuging at room temperature to obtain supernatant;
(3) adjusting the pH of the supernatant in the step (2) to be neutral, adding an ethanol solution of a mulberry leaf flavone extraction surfactant, an ethanol solution of a mulberry leaf polysaccharide extraction surfactant and an ethanol solution of a mulberry leaf alkaloid extraction surfactant, performing ultrasonic treatment, extracting, and evaporating to dryness to obtain a mulberry leaf active substance.
2. The method for extracting active substances from mulberry leaves according to claim 1, wherein the solution with pH of 3.5-6.5 in the step (1) is diluted hydrochloric acid or diluted sulfuric acid solution, and the ratio of the volume of the solution to the mass of the mulberry leaf ultrafine powder is 15-20mL:1 g.
3. The method for extracting active substances from mulberry leaves as claimed in claim 1, wherein the ultrasonic treatment conditions of the step (1) are as follows: performing ultrasonic treatment at room temperature under the conditions of 150 and 200W for 40-60 min.
4. The method for extracting active substances from mulberry leaves according to claim 1, wherein the mass ratio of the mulberry leaf ultrafine powder and the cellulase in the step (1) is 100: 2-5.
5. The method as claimed in claim 1, wherein the number of times of the alternate deposition at the temperature in step (2) is determined by ninhydrin test until ninhydrin test in the lower clear liquid is negative.
6. The method as claimed in claim 1, wherein in the step (3), the surfactant in the ethanol solution of the mulberry leaf flavonoid extraction surfactant is triton X-100, and the mass concentration of the ethanol solution of the mulberry leaf flavonoid extraction surfactant is 3-5%.
7. The method as claimed in claim 1, wherein in the step (3), the surfactant in the ethanol solution of the mulberry leaf polysaccharide extracting surfactant is one of sodium dodecyl sulfate, cetyl trimethyl ammonium bromide and octyl phenol polyoxyethylene ether-10, and the mass concentration of the ethanol solution of the mulberry leaf polysaccharide extracting surfactant is 1-3%.
8. The method for extracting active substances from mulberry leaves according to claim 1, wherein in the step (3), the surfactant in the ethanol solution of the mulberry leaf alkaloid extraction surfactant is one of tween 80, silk disc 80 and APG0801, and the mass concentration of the ethanol solution of the mulberry leaf alkaloid extraction surfactant is 2-4%.
9. The method for extracting active substances from mulberry leaves as claimed in claim 1, wherein the ultrasonic treatment conditions in the step (3) are as follows: performing ultrasonic treatment at 25 + -5 deg.C under 80-120W for 10-25 min.
10. The method for extracting active substances from mulberry leaves according to claim 1, wherein in the step (3), the ratio of the total amount of the ethanol solution of the mulberry leaf alkaloid extraction surfactant, the ethanol solution of the mulberry leaf polysaccharide extraction surfactant and the ethanol solution of the mulberry leaf flavone extraction surfactant to the mass of the supernatant is 0.5-0.8: 1, the volume fraction of the ethanol solution is 50-70%.
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Cited By (2)
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|---|---|---|---|---|
| CN114403445A (en) * | 2022-01-26 | 2022-04-29 | 四川默森药业有限公司 | Extraction of mulberry leaf flavone and application of mulberry leaf flavone in functional food |
| CN114712416A (en) * | 2022-01-24 | 2022-07-08 | 华中农业大学 | Method for efficiently and synchronously extracting flavone, alkaloid and polyphenol from lotus leaves by water medium method |
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|---|---|---|---|---|
| CN109627350A (en) * | 2018-11-07 | 2019-04-16 | 兰溪市哥特生物技术有限公司 | A kind of extracting method of mulberry leaf active material |
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2021
- 2021-08-26 CN CN202110989617.0A patent/CN113577138A/en active Pending
- 2021-12-14 ZA ZA2021/10387A patent/ZA202110387B/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109627350A (en) * | 2018-11-07 | 2019-04-16 | 兰溪市哥特生物技术有限公司 | A kind of extracting method of mulberry leaf active material |
Non-Patent Citations (2)
| Title |
|---|
| 李慧婷等: "浸提辅助剂在中药提取中的应用及研究进展", 《中国实验方剂学杂志》 * |
| 董树国等: "酶解_超声波_表面活性剂协同提取桑叶总黄酮工艺研究", 《中国酿造》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114712416A (en) * | 2022-01-24 | 2022-07-08 | 华中农业大学 | Method for efficiently and synchronously extracting flavone, alkaloid and polyphenol from lotus leaves by water medium method |
| CN114403445A (en) * | 2022-01-26 | 2022-04-29 | 四川默森药业有限公司 | Extraction of mulberry leaf flavone and application of mulberry leaf flavone in functional food |
Also Published As
| Publication number | Publication date |
|---|---|
| ZA202110387B (en) | 2022-02-23 |
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