CN112449977A - Planting method of tea tree rich in beta-nicotinamide mononucleotide and tea rich in beta-nicotinamide mononucleotide - Google Patents
Planting method of tea tree rich in beta-nicotinamide mononucleotide and tea rich in beta-nicotinamide mononucleotide Download PDFInfo
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Abstract
The invention relates to the technical field of tea functionality and planting methods, in particular to a planting method of tea rich in beta-nicotinamide mononucleotide and tea rich in beta-nicotinamide mononucleotide, which comprises the following steps: (1) selecting proper tea trees and planting the tea trees in proper land; (2) applying a certain amount of beta-nicotinamide mononucleotide base fertilizer after ditching in winter; secondly, applying beta-nicotinamide mononucleotide base fertilizer before picking tea trees, and simultaneously watering diatom water; (3) watering the tea trees with diatom water 6-10 days before picking; (4) and spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves twice before tea leaf picking. The invention transforms the expensive beta-nicotinamide mononucleotide into popular tea which can be drunk by common people through technical measures, thereby fully meeting the requirements of people on health. The invention has reasonable cost, popularization and good effect.
Description
Technical Field
The invention relates to the technical field of tea functionality and planting methods, in particular to a planting method of tea rich in beta-nicotinamide mononucleotide and tea rich in beta-nicotinamide mononucleotide.
Background
Tea, as one of the most long-standing beverages in China, is called three ethanol-free beverages together with coffee and cocoa in the world. Spring tea is the main domestic consumption form, and the development of the tea industry is hindered by the production mode that the tea pays attention to the backward yield; at the present stage, the tea industry of China has been transited from the 'heavy quantity' to the healthy development stage of the tea functionality, and the tea functionality becomes a key factor influencing the rapid development of the tea industry. The chemical components and trace element contents in the fresh leaves are the material basis of the tea quality and are also the key characteristics for measuring the quality of the tea, wherein the chemical components such as tea polyphenol, flavone amino acid, caffeine, soluble sugar and the like are internationally recognized specific evaluation indexes.
NMN is a fully known nicotinamid mononuleotide, nicotinamide mononucleotide, a naturally occurring bioactive nucleotide, and has 2 forms of irregular presence, α and β, NMN; the beta isomer is the active form of NMN and has a molecular weight of 334.221 g/mol.
NMN is a precursor of NAD +, the function of which is also mainly represented by NAD +, and therefore NAD + is explained first:
NAD + is also known as coenzyme, and is known as nicotinamide adenine dinucleotide, which is widely distributed in all cells of the human body, participates in thousands of biocatalytic reactions, and is an essential coenzyme in the human body.
The specific reactions in which NAD + participates are mainly the following: growth, DNA repair (PARPs mediated), SIRTs protein, NADP (H) synthesis.
The metabolism of NADP (H) is delayed compared to NAD (H), which does not mean a slower conversion rate of NADP (H), but means that NADP (H) is the downstream reaction of NAD (H), because of the very stable "time difference" between the two reactions.
The PARPs pathway:
the size of the NADP (H) pool is only 1/20 of the NAD (H) pool, the proportion of NAD + consumed by NAD kinase, normally around 10% of total NAD +, 12 pmol/million cells/hour, while the total consumption of NAD + is about 118 pmol/million cells/hour.
The PARPs pathway:
under normal conditions PARPs consume approximately 1/3 NAD +, and when DNA damage requires repair, consumption of PARPs will dominate even more.
The SIRTs pathway:
SIRTs consumed approximately 1/3 NAD +, about 32 pmol/million cells/hour under normal conditions, in a ratio similar to PARPs.
sonic super: NAD + repair dnazhuanlan
With age, NMN and NAD + levels decline on average, while NAD + metabolite NAM rises.
The decline of NAD + during aging is considered to be a major cause of diseases and disabilities such as hearing and vision loss, cognitive and motor dysfunction, immune deficiency, arthritis due to dysregulation of autoimmune inflammatory responses, metabolic disorders and cardiovascular diseases.
A great deal of research published in the International science journal Cell and Nature finds that: NMN is effective in delaying various problems caused by aging. Recent research shows that the NMN level in organisms is regulated to have better treatment and repair effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases, aging degenerative diseases and the like.
With the rapid development of modern science and technology and the gradual disclosure of aging mechanism in the scientific community, the related results of reversing aging and prolonging life make substantial breakthrough after the 21 st century.
Studies have demonstrated that NAD content in vivo decreases with age; in recent years the academic community has achieved a general consensus on the mechanisms underlying aging development: that is, aging is fundamentally caused in that DNA damage in cells is accumulated with the increase of age, resulting in the loss of cell function and regeneration ability. The NMN can finally realize the effect of reversing aging by obviously improving the repair capability of DNA damage in cells.
The biggest breakthrough began in 2015, which was first identified by Ciclel, a coenzyme β -Nicotinamide Mononucleotide (NMN) responsible for gene repair in organisms, by professor David, Harvard medical college, which could significantly reverse mammalian aging and prolong the lifespan by more than 30%. The method becomes a double hotspot in the academic and biotechnology fields, so that on one hand, the paper is harvested in the aging medical field, and on the other hand, the paper is disputed and commercialized by a plurality of biotechnology and medicine enterprises.
Researchers tested whether employing Nicotinic Acid (NA) could prevent oocyte cleavage defects during in vitro culture, they obtained fully grown immature oocytes from mice and cultured in complete medium supplemented with nicotinic acid. In old oocytes treated with NA, both ROS levels and spindle/chromosome defects were reduced, indicating that in vitro NA administration could improve the quality of older oocytes.
Recently, a study on Nature Metabolism has historically revealed to the academic world the reason for the decay of NAD + which is an important energy product during the aging process of the human body.
In 2017, Baylor College of Medicine in M nations discovered in experiments that there was a potential connection between NAD + and age change. The Hisayuki Amano research team released on Cell Metabolism in the same year, and the increased life cycle was found to be 20%. After that, the related technology is quickly transformed by the local biological enterprise gene harbor, and the large-scale mass production is realized.
However, despite the reiteration of research and transformation efforts, the academia has not always explained why this substance decreases with aging. In this study, this puzzle total was solved.
Researchers at the baker's aging institute, carefully analyzing the in vivo status of aged mice, have shown that many tissues exhibit pro-inflammatory states, resulting in extensive activation of immune cells therein. It is these cells that consume large amounts of tissue NAD + so that "starvation" results in a gradual decay of NAD + levels in mice.
Wim van Schooten also found in experiments that NAD + was restored in tissues of aged animals after the activity of these cells was reduced by antibodies, as shown in figure 1. He believes that this may be an effective anti-aging strategy.
A large number of studies at home and abroad show that the stems, branches and leaves of the tea trees have strong absorption capacity and can absorb liquid nutrient elements. As for their absorption pathways, two main ones have been found. Firstly, the nutrients are directly fed into the tea leaves through the pores on the tea leaves. Second, nutrients are absorbed by passing through the cuticle on the surface of the leaves of the tea plant and then penetrating into the protoplasm of the leaf epidermal cells. The stratum corneum on the front side of the tea leaf is thicker than that on the back side of the leaf, and the pores are mainly distributed on the back side of the leaf, so that the absorption capacity of the back side of the leaf is about 5-8 times larger than that of the front side of the leaf. In addition, the cuticle on the old leaves is thicker than that of the young leaves, so the old leaves of the tea tree have lower absorption capacity than the young leaves. Japanese research found that tea leaves can absorb substances in ionic form, such as ammonium ion (NH), when absorbing nutrients by diffusion and osmosis+ 4) Nitrate ion (NO)Ag) Potassium ion (K)+) Phosphoric acid ion (PO)4 A 3) And can absorb some molecular substances, such as urea [ (NH)2CO]And a functional nutrient solution.
At present, the functional development of tea leaves related to longevity is seriously insufficient, the synthesis amount of beta-Nicotinamide Mononucleotide (NMN) in a human body is very small, and the beta-nicotinamide mononucleotide gradually decreases with the increase of age; therefore, to satisfy the human desire for longevity, β -Nicotinamide Mononucleotide (NMN) must be replenished every day. beta-Nicotinamide Mononucleotide (NMN) is expensive and belongs to a life-prolonging tonic which is drunk by rich people. The present invention is directed to the pain spots where only a few rich people can eat beta-Nicotinamide Mononucleotide (NMN). The tea leaves are rich in beta-Nicotinamide Mononucleotide (NMN) through technical measures, so that the special long-life tonic which can be eaten by people is converted into the tea leaves which can be drunk by common people to fulfill the desire of long life.
Disclosure of Invention
The invention aims to provide a planting method of tea trees rich in beta-nicotinamide mononucleotide, which converts expensive beta-Nicotinamide Mononucleotide (NMN) into popular tea which can be drunk by common people through technical measures, and fully meets the requirement of people on health.
The second purpose of the invention is to provide tea rich in beta-nicotinamide mononucleotide.
The scheme adopted by the invention for realizing one of the purposes is as follows: a planting method of tea trees rich in beta-nicotinamide mononucleotide comprises the following steps:
(1) according to the characteristic that the tea tree roots and leaves absorb nutrients, selecting proper tea trees and planting the proper tea trees in proper land;
(2) after digging holes of tea trees in winter, applying a certain amount of beta-nicotinamide mononucleotide base fertilizer; then, two months before tea trees are picked, applying beta-nicotinamide mononucleotide base fertilizer to tea tree soil every 15-20 days, and simultaneously watering diatom water; replenishing the beta-nicotinamide mononucleotide base fertilizer in the last time 20-30 days before the harvest, and simultaneously watering the diatom water;
(3) watering the tea trees with diatom water 6-10 days before picking;
(4) spraying beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the first time 13-15 days before tea leaf picking; and spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the second time 3-5 days before tea leaf picking.
Preferably, in the step (1), the tea plant comprises at least one of green tea, yellow tea, black tea, white tea, black tea and oolong tea; the green tea comprises at least one of Longjing tea, Xinyang Maojian tea, Biluochun tea, Yulu tea and picked flower; black tea comprises clovershrub; the black tea comprises Pu' er tea; oolong tea comprises rock tea and/or cinnamon; the land selection has the altitude of between 400 and 2000 meters, positive slope, acidic pH value and mild climate.
Preferably, in the step (2), the application amount of the beta-nicotinamide mononucleotide base fertilizer after hole digging in winter is 30-40Kg per mu; the beta-nicotinamide mononucleotide base fertilizer is applied in the second month before picking in spring, and the application amount is 60-80Kg per mu.
Preferably, in the steps (2) and (3), the spraying amount of the diatom water is 800-1200 liters per mu.
Preferably, in the step (4), the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 30-40 ppm; the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the second time is 40-50 ppm, and the spraying amount of the two times is 50-60kg per mu.
Preferably, in the step (2), the formulation of the beta-nicotinamide mononucleotide base fertilizer comprises the following components in percentage by mass: 30-40 parts of phellinus igniarius waste bacteria sticks, 10-20 parts of mushroom waste bacteria sticks, 5-10 parts of grifola frondosa waste bacteria sticks, 1.5 parts of high-temperature type ferment bacteria and probiotics, 25-28 parts of animal manure, 0.5 part of traditional Chinese medicine high-positive and high-negative dreg fertilizer and beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm1-5, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55-65% of the mass of the total beta-nicotinamide mononucleotide base fertilizer.
Preferably, the preparation of the beta-nicotinamide mononucleotide based fertilizer comprises the following steps: crushing the phellinus igniarius waste bacteria sticks, the shiitake mushroom waste bacteria sticks and the grifola frondosa waste bacteria sticks, adding animal wastes and high-positive and high-negative herb residue fertilizers, uniformly mixing, adding high-temperature type ferment bacteria and probiotics, continuously stirring uniformly to obtain a beta-nicotinamide mononucleotide base fertilizer raw material, stacking the obtained beta-nicotinamide mononucleotide base fertilizer raw material, and adding water while feeding one layer by one layer during stacking; after the building of the compost is finished, inserting a plurality of vent pipes into the compost, finally sealing the compost, opening the seal after 6-8 days, turning over the compost once, and repeating the operation for a plurality of times to prepare the beta-nicotinamide mononucleotide base fertilizer.
Preferably, in the step (4), the preparation method of the functional nutrient solution of beta-nicotinamide mononucleotide comprises the following steps: according to the weight parts, 70-80 parts of beta-nicotinamide mononucleotide is dissolved by warm water at 50-60 ℃, 3-8 parts of yin essence and yang essence life mineral fertilizer and 12-27 parts of diatom water are added and uniformly mixed to obtain the beta-nicotinamide mononucleotide functional nutrient solution, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution is ppm 30-50.
Preferably, the preparation method of the yin-essence and yang-essence life mineral fertilizer comprises the following steps: each kilogram of yin-essence yang-essence life mineral fertilizer contains 10-15 mg of yin-essence iron, 0.15 mg of yang-essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum.
The second scheme adopted by the invention for achieving the purpose is as follows: tea rich in beta-nicotinamide mononucleotide is planted by adopting the planting method, and the content of the beta-nicotinamide mononucleotide in the tea rich in the beta-nicotinamide mononucleotide is 5-30 mg/Kg.
The invention has the following advantages and beneficial effects:
the invention utilizes the principle that the leaf surfaces of tea trees absorb nutrient elements to prepare a base fertilizer and a functional nutrient solution which mainly adopt beta-nicotinamide mononucleotide functionality, applies beta-nicotinamide mononucleotide base fertilizer to the root parts of the tea trees in autumn, winter and spring, sprays beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves twice before the tea leaves are harvested, converts expensive beta-nicotinamide mononucleotide into popular tea leaves which can be drunk by common people through technical measures, and fully meets the requirement of people on health.
The tea leaves planted by the planting method of the invention are manufactured conventionally, and the content of beta-Nicotinamide Mononucleotide (NMN) in the tea leaves is 5-30 mg/kg. The invention has reasonable cost, popularization and good effect.
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FIG. 1 is a report of the detection of the content of β -nicotinamide mononucleotide in tea leaves planted in example 1 of the present invention.
Detailed Description
The following examples are provided to further illustrate the present invention for better understanding, but the present invention is not limited to the following examples.
Example 1
A planting method of tea trees rich in beta-nicotinamide mononucleotide comprises the following planting steps:
(1) selecting tea trees and planting fields: the Longjing is selected and planted at the altitude of 600 plus 1800 meters, the slope is positive, the pH value is acidic, the land is more than 200 mu, and the climate is mild.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the hole distance between every two tea trees is 15-20 cm, the furrow depth is 40 cm, applying organic beta-nicotinamide mononucleotide base fertilizer with the fertilizer amount of 30Kg per mu, then applying 70Kg of beta-nicotinamide mononucleotide base fertilizer containing base fertilizer to the tea tree soil and covering the soil with soil every 18 days two months before the tea trees are picked, simultaneously pouring diatom water once, wherein the spraying amount of the diatom water is 1000 liters per mu, keeping the tea tree soil moist, and facilitating the absorption of the tea tree roots; the last time of the last application of the beta-nicotinamide mononucleotide base fertilizer 20-30 days before the harvest, wherein the application amount of the beta-nicotinamide mononucleotide base fertilizer is 70Kg per mu, and meanwhile, the diatom water is poured, and the spraying amount of the diatom water is 1000 liters per mu;
(3) watering the tea trees with diatom water 8 days before picking, wherein the spraying amount of the diatom water is 1000 liters per mu;
(4) spraying beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the first time 13-15 days before tea leaf picking; 3-5 days before tea leaf picking, spraying a beta-nicotinamide mononucleotide functional nutrient solution to the tea leaf for the second time, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution for the first time is ppm 35; the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the second time is ppm45, and the spraying amount of the two times is 55kg per mu.
The preparation method of the beta-nicotinamide mononucleotide-containing base fertilizer in the embodiment comprises the following steps:
step one, crushing 35 parts of phellinus igniarius waste bacteria sticks, 15 parts of mushroom waste bacteria sticks and 7 parts of grifola frondosa waste bacteria sticks in parts by weight, adding 27 parts of animal wastes and 0.5 part of traditional Chinese medicine high-positive and high-negative dreg fertilizer, uniformly mixing, adding 1.5 parts of high-temperature enzyme bacteria and probiotics, and uniformly stirring to obtain the raw material of the beta-nicotinamide mononucleotide based fertilizer.
And step two, piling the obtained raw materials of the beta-nicotinamide mononucleotide based fertilizer, feeding the raw materials layer by layer during piling, adding beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm3 while feeding, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55-60% of the mass of the total beta-nicotinamide mononucleotide based fertilizer, inserting the raw materials into the compost by 4 stainless steel pipes with holes after the piling is finished, finally covering the compost with a plastic film for sealing, opening a sealing film after 7 days, and turning the compost once by using a pile-turning machine. Turning over once in 7 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the functional nutrient solution containing beta-nicotinamide mononucleotide in the embodiment is as follows: the weight portions are as follows: dissolving 75 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for later use; and adding 5 parts of yin essence and yang essence life mineral fertilizer and 20 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, finally, placing the beta-nicotinamide mononucleotide functional nutrient solution into a temperature of 4-8 ℃ for storage for later use, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of ppm30-50 according to requirements.
The preparation method of the yin-essence and yang-essence life mineral fertilizer in the embodiment is as follows: each kg of the capsule contains 10-15 mg of yin essence iron, 0.15 mg of yang essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water at high temperature and high pressure to obtain the water-soluble mineral fertilizer. The life activity elements belong to immune elements in medicine, wherein elements such as positive manganese zinc molybdenum selenium and negative iron play roles in catalyzing and activating tea life cells and promoting the metabolism of tea biological enzymes.
In this embodiment, the diatom water is the prior art, i.e., the water in which diatoms grow is diatom water.
The report of the detection of the content of beta-nicotinamide mononucleotide in longjing tea leaves planted in this example is shown in fig. 1, and the report of the detection in fig. 1 shows that the content of beta-nicotinamide mononucleotide in each kilogram of tea leaves is 2.01 x 104Mu g, which shows that the planting method of the invention is effective and can successfully obtain the tea rich in beta-nicotinamide mononucleotide.
Example 2
A tea rich in beta-nicotinamide mononucleotide and a planting method thereof comprise the following planting steps:
(1) selecting tea trees and planting fields: selecting Xinyang Maojian tea, planting the Xinyang Maojian tea at the altitude of 400-2000 m, having positive slope, acidic pH value, more than 200 mu land with a continuous land and mild climate.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the hole distance between every two tea trees is 15-20 cm, the furrow depth is 40 cm, applying fertilizer amount is 30Kg per mu, then applying 60Kg of beta-nicotinamide mononucleotide based fertilizer to the tea tree soil two months before the tea trees are picked, covering the tea tree soil with soil every 15 days, and simultaneously watering once with diatom water, wherein the spraying amount of the diatom water is 800 liters per mu, so that the tea tree soil is kept moist, and the absorption of the tea tree roots is facilitated; the last time of the last 20-30 days before the harvest, the supplement application amount of the beta-nicotinamide mononucleotide base fertilizer is 60Kg per mu, and simultaneously, the diatom water is poured, and the spraying amount of the diatom water is 800L per mu.
(3) Watering the tea trees with diatom water 6 days before picking, wherein the spraying amount of the diatom water is 800 liters per mu;
(4) spraying beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the first time 13-15 days before tea leaf picking; 3-5 days before tea leaf picking, spraying a beta-nicotinamide mononucleotide functional nutrient solution to the tea leaf for the second time, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution for the first time is ppm 30; the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the second time is ppm40, and the spraying amount of the two times is 60kg per mu.
The preparation method of the beta-nicotinamide mononucleotide-containing base fertilizer in the embodiment comprises the following steps:
step one, crushing 30 parts of phellinus igniarius waste bacteria sticks, 10 parts of mushroom waste bacteria sticks and 5 parts of grifola frondosa waste bacteria sticks in parts by weight, adding 25 parts of animal manure and 0.5 part of traditional Chinese medicine high-positive and high-negative dreg fertilizer, uniformly mixing, adding 1.5 parts of high-temperature enzyme bacteria and probiotics, and uniformly stirring to obtain the raw material of the beta-nicotinamide mononucleotide based fertilizer.
And step two, piling the obtained raw materials of the beta-nicotinamide mononucleotide based fertilizer, feeding the raw materials layer by layer during piling, adding beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm1 while feeding, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55-60% of the mass of the total beta-nicotinamide mononucleotide based fertilizer, inserting the raw materials into the compost by 4 stainless steel pipes with holes after the piling is finished, finally covering the compost with a plastic film for sealing, opening a sealing film after 7 days, and turning the compost once by using a pile-turning machine. Turning over once in 6 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the functional nutrient solution containing beta-nicotinamide mononucleotide in the embodiment is as follows: the weight portions are as follows: dissolving 70 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for later use; and adding 3 parts of yin essence and yang essence life mineral fertilizer and 12 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, finally, placing the beta-nicotinamide mononucleotide functional nutrient solution into a temperature of 4-8 ℃ for storage for later use, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of ppm30-50 according to requirements.
The preparation method of the yin-essence and yang-essence life mineral fertilizer in the embodiment is as follows: each kg of the capsule contains 10-15 mg of yin essence iron, 0.15 mg of yang essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water at high temperature and high pressure to obtain the water-soluble mineral fertilizer. The life activity elements belong to immune elements in medicine, wherein elements such as positive manganese zinc molybdenum selenium and negative iron play roles in catalyzing and activating tea life cells and promoting the metabolism of tea biological enzymes.
In this embodiment, the diatom water is the prior art, i.e., the water in which diatoms grow is diatom water.
Example 3
(1) Selecting tea trees and planting fields: puer is selected and planted at the altitude of 400-2000 m, the slope is positive, the pH value is acidic, the land is more than 200 mu, and the climate is mild.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the hole distance between every two tea trees is 15-20 cm, the furrow depth is 40 cm, applying organic beta-nicotinamide mononucleotide base fertilizer, the fertilizer amount is 40Kg per mu, then applying 80Kg of beta-nicotinamide mononucleotide base fertilizer containing base fertilizer to the tea tree soil and covering the soil with the soil every 20 days two months before the tea trees are picked, simultaneously pouring diatom water once, and the spraying amount of the diatom water is 1200L per mu, so that the tea tree soil is kept moist, and the absorption of the roots of the tea trees is facilitated; the last time of the last application of the beta-nicotinamide mononucleotide base fertilizer 20-30 days before the harvest, wherein the application amount of the beta-nicotinamide mononucleotide base fertilizer is 80Kg per mu, and meanwhile, the diatom water is poured, and the spraying amount of the diatom water is 1200L per mu;
(3) watering the tea trees with diatom water 10 days before picking, wherein the spraying amount of the diatom water is 1200 liters per mu;
(4) spraying beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the first time 13-15 days before tea leaf picking; 3-5 days before tea leaf picking, spraying a beta-nicotinamide mononucleotide functional nutrient solution to the tea leaf for the second time, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution for the first time is ppm 40; the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the second time is ppm50, and the spraying amount of the two times is 60kg per mu.
The preparation method of the beta-nicotinamide mononucleotide-containing base fertilizer in the embodiment comprises the following steps:
step one, crushing 40 parts of phellinus igniarius waste bacteria sticks, 20 parts of mushroom waste bacteria sticks and 10 parts of grifola frondosa waste bacteria sticks in parts by weight, adding 28 parts of animal wastes and 0.5 part of traditional Chinese medicine high-positive and high-negative dreg fertilizer, uniformly mixing, adding 1.5 parts of high-temperature enzyme bacteria and probiotics, and uniformly stirring to obtain the raw material of the beta-nicotinamide mononucleotide based fertilizer.
And step two, piling the obtained raw materials of the beta-nicotinamide mononucleotide based fertilizer, feeding the raw materials layer by layer during piling, adding beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm5 while feeding, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55-60% of the mass of the total beta-nicotinamide mononucleotide based fertilizer, inserting the raw materials into the compost by 4 stainless steel pipes with holes after the piling is finished, finally covering the compost with a plastic film for sealing, opening a sealing film after 7 days, and turning the compost once by using a pile-turning machine. Turning over once in 8 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the functional nutrient solution containing beta-nicotinamide mononucleotide in the embodiment is as follows: the weight portions are as follows: dissolving 80 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for later use; and adding 8 parts of yin essence and yang essence life mineral fertilizer and 27 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, finally, placing the beta-nicotinamide mononucleotide functional nutrient solution into a temperature of 4-8 ℃ for storage for later use, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of ppm30-50 according to requirements.
The preparation method of the yin-essence and yang-essence life mineral fertilizer in the embodiment is as follows: each kg of the capsule contains 10-15 mg of yin essence iron, 0.15 mg of yang essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water at high temperature and high pressure to obtain the water-soluble mineral fertilizer. The life activity elements belong to immune elements in medicine, wherein elements such as positive manganese zinc molybdenum selenium and negative iron play roles in catalyzing and activating tea life cells and promoting the metabolism of tea biological enzymes.
In this embodiment, the diatom water is the prior art, i.e., the water in which diatoms grow is diatom water.
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (10)
1. A planting method of tea trees rich in beta-nicotinamide mononucleotide is characterized by comprising the following steps:
(1) according to the characteristic that the tea tree roots and leaves absorb nutrients, selecting proper tea trees and planting the proper tea trees in proper land;
(2) after digging holes of tea trees in winter, applying a certain amount of beta-nicotinamide mononucleotide base fertilizer; then, two months before tea trees are picked, applying beta-nicotinamide mononucleotide base fertilizer to tea tree soil every 15-20 days, and simultaneously watering diatom water; replenishing the beta-nicotinamide mononucleotide base fertilizer in the last time 20-30 days before the harvest, and simultaneously watering the diatom water;
(3) watering the tea trees with diatom water 6-10 days before picking;
(4) spraying beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the first time 13-15 days before tea leaf picking; and spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the second time 3-5 days before tea leaf picking.
2. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the step (1), the tea tree comprises at least one of green tea, yellow tea, black tea, white tea, black tea and oolong tea; the green tea comprises at least one of Longjing tea, Xinyang Maojian tea, Biluochun tea, Yulu tea and picked flower; black tea comprises clovershrub; the black tea comprises Pu' er tea; oolong tea comprises rock tea and/or cinnamon; the land selection has the altitude of between 400 and 2000 meters, positive slope, acidic pH value and mild climate.
3. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the step (2), the application amount of the beta-nicotinamide mononucleotide base fertilizer after hole digging in winter is 30-40Kg per mu; the beta-nicotinamide mononucleotide base fertilizer is applied in the second month before picking in spring, and the application amount is 60-80Kg per mu.
4. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the steps (2) and (3), the spraying amount of the diatom water is 800-1200 liters per mu.
5. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the step (4), the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the first time is ppm 30-40; the concentration of the functional nutrient solution of beta-nicotinamide mononucleotide sprayed for the second time is 40-50 ppm, and the spraying amount of the two times is 50-60kg per mu.
6. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the step (2), the formula of the beta-nicotinamide mononucleotide base fertilizer comprises the following components in percentage by mass: 30-40 parts of phellinus igniarius waste bacteria sticks, 10-20 parts of mushroom waste bacteria sticks, 5-10 parts of grifola frondosa waste bacteria sticks, 1.5 parts of high-temperature type ferment bacteria and probiotics, 25-28 parts of animal manure, 0.5 part of traditional Chinese medicine high-positive and high-negative dreg fertilizer and beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm1-5, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55-65% of the mass of the total beta-nicotinamide mononucleotide base fertilizer.
7. The method for planting tea trees enriched in β -nicotinamide mononucleotide as claimed in claim 6, wherein: the preparation method of the beta-nicotinamide mononucleotide base fertilizer comprises the following steps: crushing the phellinus igniarius waste bacteria sticks, the shiitake mushroom waste bacteria sticks and the grifola frondosa waste bacteria sticks, adding animal wastes and high-positive and high-negative herb residue fertilizers, uniformly mixing, adding high-temperature type ferment bacteria and probiotics, continuously stirring uniformly to obtain a beta-nicotinamide mononucleotide base fertilizer raw material, stacking the obtained beta-nicotinamide mononucleotide base fertilizer raw material, and adding water while feeding one layer by one layer during stacking; after the building of the compost is finished, inserting a plurality of vent pipes into the compost, finally sealing the compost, opening the seal after 6-8 days, turning over the compost once, and repeating the operation for a plurality of times to prepare the beta-nicotinamide mononucleotide base fertilizer.
8. The method for planting tea trees rich in beta-nicotinamide mononucleotide according to claim 1, wherein the method comprises the following steps: in the step (4), the preparation method of the beta-nicotinamide mononucleotide functional nutrient solution comprises the following steps: according to the weight parts, 70-80 parts of beta-nicotinamide mononucleotide is dissolved by warm water at 50-60 ℃, 3-8 parts of yin essence and yang essence life mineral fertilizer and 12-27 parts of diatom water are added and uniformly mixed to obtain the beta-nicotinamide mononucleotide functional nutrient solution, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution is ppm 30-50.
9. The method for growing tea trees enriched in β -nicotinamide mononucleotide according to claim 8, wherein: the preparation method of the yin-essence and yang-essence life mineral fertilizer comprises the following steps: each kilogram of yin-essence yang-essence life mineral fertilizer contains 10-15 mg of yin-essence iron, 0.15 mg of yang-essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum.
10. Tea leaves rich in beta-nicotinamide mononucleotide are characterized in that: planting by the planting method of any one of claims 1-9, wherein the tea leaves rich in beta-nicotinamide mononucleotide has a beta-nicotinamide mononucleotide content of 5-30 mg/Kg.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115735686A (en) * | 2022-11-29 | 2023-03-07 | 福大生命科技(广州)有限公司 | Cultivation method for increasing Nicotinamide Mononucleotide (NMN) content in broccoli |
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| CN100998288A (en) * | 2007-01-04 | 2007-07-18 | 卢万俊 | Method for breeding wild tea tree |
| CN102599305A (en) * | 2012-03-08 | 2012-07-25 | 刘茜 | Selenium-rich chrysanthemum tea or tea |
| CN105191631A (en) * | 2015-09-24 | 2015-12-30 | 广西百润源农业有限公司 | High-yield planting method for pollution-free tea |
| CA3060371A1 (en) * | 2017-04-28 | 2018-11-01 | Nichia Corporation | Method for increasing amount of phenolic compound in plant |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998288A (en) * | 2007-01-04 | 2007-07-18 | 卢万俊 | Method for breeding wild tea tree |
| CN102599305A (en) * | 2012-03-08 | 2012-07-25 | 刘茜 | Selenium-rich chrysanthemum tea or tea |
| CN105191631A (en) * | 2015-09-24 | 2015-12-30 | 广西百润源农业有限公司 | High-yield planting method for pollution-free tea |
| CA3060371A1 (en) * | 2017-04-28 | 2018-11-01 | Nichia Corporation | Method for increasing amount of phenolic compound in plant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115735686A (en) * | 2022-11-29 | 2023-03-07 | 福大生命科技(广州)有限公司 | Cultivation method for increasing Nicotinamide Mononucleotide (NMN) content in broccoli |
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