CN112449977B - Planting method of tea leaves rich in beta-nicotinamide mononucleotide tea trees and tea leaves rich in beta-nicotinamide mononucleotide - Google Patents
Planting method of tea leaves rich in beta-nicotinamide mononucleotide tea trees and tea leaves rich in beta-nicotinamide mononucleotide Download PDFInfo
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Abstract
The invention relates to the technical field of tea functionality and planting methods, in particular to a planting method of tea rich in beta-nicotinamide mononucleotide tea trees and tea rich in beta-nicotinamide mononucleotide, comprising the following steps of: (1) selecting proper tea trees and planting the proper tea trees on proper lands; (2) Applying a certain amount of beta-nicotinamide mononucleotide base fertilizer after ditching in winter; then, supplementing beta-nicotinamide mononucleotide base fertilizer before picking tea trees, and pouring diatom water; (3) watering said tea tree with diatom water 6-10 days prior to harvest; (4) And spraying the beta-nicotinamide mononucleotide functional nutrient solution on tea leaves twice before tea leaf picking. The invention converts expensive beta-nicotinamide mononucleotide into a popular tea which can be drunk by common people through technical measures, and fully meets the requirements of people on health. The invention has reasonable cost, popularization and good effect.
Description
Technical Field
The invention relates to the technical field of tea functionality and planting methods, in particular to a planting method of tea trees rich in beta-nicotinamide mononucleotide and tea rich in beta-nicotinamide mononucleotide.
Background
Tea is one of the most long-history beverages in China, and is called three ethanol-free beverage in the world together with coffee cocoa. Spring tea is a main consumption form in China, and the production mode of the tea leaves with the laggard yield is focused to prevent the development of the tea industry; at present, the tea industry in China is transited from heavy quantity to the healthy development stage of tea functionality, and the tea functionality becomes a key factor affecting the rapid development of the tea industry. The chemical components and trace element content in fresh leaves are the material basis of tea quality and are also key characteristics for measuring the quality of tea, wherein the chemical components such as tea polyphenol, flavone amino acid, caffeine, soluble sugar and the like are internationally recognized specific evaluation indexes.
NMN full name nicotinamide mononucleotide, namely nicotinamide mononucleotide, is a naturally occurring bioactive nucleotide, NMN has 2 irregularly existing forms, alpha and beta; the beta isomer is an active form of NMN and has a molecular weight of 334.221g/mol.
NMN is a precursor of nad+ and its function is also predominantly reflected by nad+, so first the nad+ is explained:
NAD+ also known as coenzyme, is known as nicotinamide adenine dinucleotide, which is widely distributed in all cells of the human body, participates in thousands of biocatalytic reactions, and is an indispensable coenzyme in the human body.
The reactions in which NAD+ is specifically involved are mainly the following: growth, DNA repair (PARPs mediated), SIRTs protein, NADP (H) synthesis.
The metabolism of NADP (H) is delayed compared to NAD (H), which does not mean that the NADP (H) conversion rate is slower, but rather that NADP (H) is a downstream reaction of NAD (H) because there is a very stable "time difference" between the two reactions.
PARPs pathway:
the NADP (H) pool is only 1/20 of the NAD (H) pool, the proportion of NAD+ consumed by NAD kinase is normally around 10% of the total NAD+, 12 pmol/million cells/hour, and the total NAD+ consumed is about 118 pmol/million cells/hour.
PARPs pathway:
PARPs consume approximately 1/3 of nad+ under normal conditions, and when DNA damage requires repair, PARPs consumption will dominate more.
SIRTs pathway:
SIRTs consume approximately 1/3 of NAD+ under normal conditions, about 32 pmol/million cells/hour, and have a similar ratio to PARPs.
sonic super: NAD+ repairs DNAzhuanlan. Zhihu. Com by these means
With age, NMN and NAD+ levels tended to decrease, while NAD+ metabolite NAM tended to rise.
The decline in nad+ during aging is believed to be a major cause of diseases and disabilities, such as hearing and vision loss, cognitive and motor dysfunction, immunodeficiency, arthritis caused by autoimmune inflammatory response disorders, metabolic disorders, and cardiovascular diseases.
A great deal of research published in International journal of science, "Cell" and "Nature" has found that: NMN is effective in delaying various problems caused by aging. Recent researches show that the composition has better treatment and repair effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases, aging degenerative diseases and the like by regulating the level of NMN in organisms.
With the rapid development of modern science and technology, the related achievements of reversing aging and prolonging life are also continuously broken through in essence after entering the 21 st century along with the gradual disclosure of aging mechanism in the scientific community.
Studies confirm that NAD content in the body decreases with age; the academic world has achieved a general consensus as to the mechanisms of aging formation in recent years: that is, the root cause of aging is that DNA damage in cells is accumulated with age, resulting in loss of cellular functions and regeneration ability. NMN can achieve the effect of aging reversal by remarkably improving the repair capability of DNA damage in cells.
The biggest breakthrough starts in 2015, and the professor of genetics of Harvard medical institute is Weixin gram of Laier for the first time, and a coenzyme beta-Nicotinamide Mononucleotide (NMN) responsible for gene repair in organisms can significantly reverse aging of mammals and prolong the service life by more than 30%. The novel biological tissue harvesting machine is instantly made into a double hot spot in the fields of academic and biotechnology, on one hand, the paper is harvested crazy in the field of aging medicine, and on the other hand, the novel biological tissue harvesting machine is commercialized by multiple biotechnologies and pharmaceutical enterprises.
Researchers tested whether the use of Nicotinic Acid (NA) during in vitro culture could prevent the division defect of oocytes, and they obtained fully grown immature oocytes from mice and cultured them in complete medium supplemented with nicotinic acid. In NA-treated old oocytes, both ROS levels and spindle/chromosome defects were reduced, indicating that in vitro NA administration can improve the quality of the old oocytes.
Recently, a study in Nature, sub-journal Nature Metabolism, historically revealed to the academy that the important energy product NAD+ decays during aging of the human body.
In 2017, M country Baylor College of Medicine found in experiments that nad+ was potentially linked to age changes. The same year Hisayuki Amano study team published on Cell metanolism, with a 20% more increased survival period. After that, the related technology is quickly transformed by the native biological enterprise gene port, thus realizing large-scale mass production.
However, although research and transformation results are additive, the academy has failed to explain why this substance declines with aging. In this study, this puzzle was solved.
Researchers at the Baker's aging institute carefully analyze the in vivo status of aged mice and as a result show that many tissues exhibit a pro-inflammatory state, resulting in a wide range of activation of immune cells therein. It is these cells that consume large amounts of nad+ in tissues, so that "supply and demand" is the gradual decay of nad+ levels in mice.
Wim van Schooten doctor also found in experiments that nad+ was restored in aged animal tissues after the activity of these cells was reduced by antibodies, as shown in fig. 1. He believes that this might be an effective anti-aging strategy.
A great deal of research at home and abroad shows that the stems, branches and leaves of the tea trees have strong absorption capacity and can absorb liquid nutrient elements. As for its absorption pathway, studies have found that there are mainly two. The first and the second nutrients are directly introduced into the leaf through the air holes on the leaf. Second, nutrients are first absorbed through the cuticle layer on the leaf blade surface of the tea tree and then permeate into the protoplasm of the leaf epidermal cells. Because the stratum corneum on the front of the leaf is thicker than the stratum corneum on the back of the leaf, and the pores are mainly distributed on the back of the leaf, the absorption capacity of the back of the leaf is about 5-8 times greater than She Zhengmian. In addition, the horny layer on the old leaves is thicker than that of the young leaves, so that the absorption capacity of the old leaves of tea trees is lower than that of the young leaves. Japanese research has found that tea leaves, when absorbing nutrients by diffusion permeation, can absorb both ionic species, such as ammonium ions (NH) + 4 ) Nitric acid ion (NO) A first part g)Potassium ion (K) + ) Phosphate ions (PO) 4 One 3 ) But also can absorb some molecular substances such as urea [ (NH) 2 CO]A functional nutrient solution.
At present, the tea has serious deficiency in long-life functional development, and the synthesis amount of beta-Nicotinamide Mononucleotide (NMN) in human bodies is very small, and gradually decreases with the increase of age; thus, to meet the human desire for longevity, it is necessary to supplement β -Nicotinamide Mononucleotide (NMN) daily. And beta-Nicotinamide Mononucleotide (NMN) is expensive, belonging to a longevity tonic for rich people. The present invention is directed to pain spots where only a small number of people can eat beta-Nicotinamide Mononucleotide (NMN). The tea is rich in beta-Nicotinamide Mononucleotide (NMN) through technical measures, so that the special long-life tonic which can be eaten by rich people is converted into tea which can be drunk by common people to achieve the long-life wish.
Disclosure of Invention
The invention aims to provide a planting method of tea rich in beta-nicotinamide mononucleotide tea trees, which converts expensive beta-Nicotinamide Mononucleotide (NMN) into popular tea which can be drunk by common people through technical measures, and fully meets the requirements of people on health.
The second object of the invention is to provide a tea leaf rich in beta-nicotinamide mononucleotide.
The scheme adopted by the invention for achieving one of the purposes is as follows: a planting method of tea leaves rich in beta-nicotinamide mononucleotide tea trees comprises the following steps:
(1) Selecting proper tea trees according to the characteristic of nutrient absorption of the root parts and leaves of the tea trees, and planting the proper tea trees on proper lands;
(2) After digging holes in winter, applying a certain amount of beta-nicotinamide mononucleotide base fertilizer to tea trees; then, supplementing beta-nicotinamide mononucleotide base fertilizer to the tea tree soil every 15-20 days two months before picking the tea tree, and simultaneously watering diatom water; the base fertilizer containing the beta-nicotinamide mononucleotide is applied for the last time 20-30 days before picking, and diatom water is poured at the same time;
(3) Pouring diatom water to the tea tree 6-10 days before harvest;
(4) Spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the first time 13-15 days before tea leaf picking; and spraying the beta-nicotinamide mononucleotide functional nutrient solution on the tea leaves for the second time 3-5 days before tea leaf picking.
Preferably, in the step (1), the tea tree comprises at least one of green tea, yellow tea, black tea, white tea, black tea and oolong tea; the green tea comprises at least one of Longjing tea, xinyang Maojian tea, biluochun tea, yulu tea and Queen tea; black tea includes Dahongpao tea; the black tea comprises Pu' er; oolong tea includes rock tea and/or cinnamon; the land is selected to have the altitude of 400-2000 m, positive gradient, acidic pH value and mild climate.
Preferably, in the step (2), the application amount of the beta-nicotinamide mononucleotide base fertilizer after digging holes in winter is 30-40 Kg/mu; and the beta-nicotinamide mononucleotide base fertilizer is supplemented two months before spring picking, and the supplementing amount is 60-80Kg per mu.
Preferably, in the steps (2) and (3), the spraying amount of the diatom water is 800-1200 liters per mu.
Preferably, in the step (4), the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 30-40 ppm; the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 40-50 ppm, and the spraying amount of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 50-60kg per mu.
Preferably, in the step (2), the formulation of the beta-nicotinamide mononucleotide base fertilizer comprises the following components in percentage by mass: 30-40 parts of Sang Huangfei fungus sticks, 10-20 parts of mushroom waste fungus sticks, 5-10 parts of grifola frondosa waste fungus sticks, 1.5 parts of high-temperature ferment fungus and probiotics, 25-28 parts of animal manure, 0.5 part of traditional Chinese medicine high-positive and high-negative medicine residue fertilizer and 1-5 ppm of beta-nicotinamide mononucleotide aqueous solution, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55% -65% of the mass of the total beta-nicotinamide mononucleotide base fertilizer.
Preferably, the preparation of the beta-nicotinamide mononucleotide base fertilizer comprises the following steps: crushing Sang Huangfei fungus sticks, mushroom waste fungus sticks and grifola frondosa waste fungus sticks, adding animal manure and traditional Chinese medicine high-positive and high-negative medicine residue fertilizers, uniformly mixing, adding high-temperature ferment bacteria and probiotics, continuously stirring uniformly to obtain a raw material of the beta-nicotinamide mononucleotide base fertilizer, and then stacking the raw materials of the beta-nicotinamide mononucleotide base fertilizer, wherein the raw materials are added layer by layer during stacking, and water is added while adding the raw materials; after the pile is built, a plurality of ventilation pipes are inserted into the compost, the compost is sealed, the sealed pile is opened and turned over once after 6-8 days, and the beta-nicotinamide mononucleotide base fertilizer can be prepared by repeating for a plurality of times.
Preferably, in the step (4), the preparation method of the beta-nicotinamide mononucleotide functional nutrient solution comprises the following steps: according to parts by weight, 70-80 parts of beta-nicotinamide mononucleotide is dissolved by adopting warm water at 50-60 ℃, 3-8 parts of yin-yang-essence life mineral fertilizer and 12-27 parts of diatom water are added, and the beta-nicotinamide mononucleotide functional nutrient solution is obtained after uniform mixing, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution is 30-50 ppm.
Preferably, the preparation method of the vital essence-yin-yang-essence life mineral fertilizer comprises the following steps: each kilogram of vital mineral fertilizer contains 10-15 mg of vital essence iron, 0.15 mg of vital essence manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum.
The scheme adopted by the invention for achieving the second purpose is as follows: the tea rich in the beta-nicotinamide mononucleotide is planted by adopting the planting method, and the content of the beta-nicotinamide mononucleotide in the tea rich in the beta-nicotinamide mononucleotide is 5-30mg/Kg.
The invention has the following advantages and beneficial effects:
according to the invention, by utilizing the principle that the leaf surfaces of tea trees absorb nutrient elements, a base fertilizer and a functional nutrient solution which mainly comprise beta-nicotinamide mononucleotide are prepared, shi Jia-nicotinamide mononucleotide base fertilizer is applied to the root of tea trees in autumn and winter and spring, and the beta-nicotinamide mononucleotide functional nutrient solution is applied to the tea leaves twice before the tea leaves are harvested, so that expensive beta-nicotinamide mononucleotide is converted into popular tea leaves through technical measures, and the health requirements of people are fully met.
The tea tree leaves planted by the planting method are manufactured according to the conventional method, and the content of beta-Nicotinamide Mononucleotide (NMN) in the tea leaves is 5-30mg/kg. The invention has reasonable cost, popularization and good effect.
Drawings
FIG. 1 is a report showing the measurement of the content of β -nicotinamide mononucleotide in tea leaves planted in example 1 of the present invention.
Detailed Description
For a better understanding of the present invention, the following examples are further illustrative of the present invention, but the contents of the present invention are not limited to the following examples only.
Example 1
A planting method of tea trees rich in beta-nicotinamide mononucleotide comprises the following planting steps:
(1) Tea tree and planting field selection: dragon well is selected, planted at the altitude of 600-1800 m, positive in gradient, acidic in pH value, and moderate in climate, and the land area is more than 200 mu.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the holes are 15-20 cm away from tea trees, the depth of the holes is 40 cm, organic beta-nicotinamide mononucleotide base fertilizer is applied, the fertilizer amount is 30Kg per mu, then 70Kg of beta-nicotinamide mononucleotide base fertilizer is applied to the tea tree soil every 18 days before tea tree picking and covered by soil, diatom water is poured once, the spraying amount of the diatom water is 1000 liters per mu, the tea tree soil is kept moist, and the tea tree root absorption is facilitated; the beta-nicotinamide mononucleotide base fertilizer is applied for the last time 20-30 days before picking, the application amount of the beta-nicotinamide mononucleotide base fertilizer is 70Kg per mu, diatom water is poured at the same time, and the spraying amount of the diatom water is 1000 liters per mu;
(3) Pouring diatom water to the tea trees 8 days before picking, wherein the spraying amount of the diatom water is 1000 liters per mu;
(4) Spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the first time 13-15 days before tea leaf picking; spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the second time 3-5 days before tea leaf picking, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 35 ppm; the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 45 ppm, and the spraying amount of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 55kg per mu.
The preparation method of the mononucleotide base fertilizer containing the beta-nicotinamide comprises the following steps:
according to the weight portions, 35 portions of Sang Huangfei fungus sticks, 15 portions of mushroom waste fungus sticks and 7 portions of grifola frondosa waste fungus sticks are crushed, 27 portions of animal manure and 0.5 portion of traditional Chinese medicine high-positive and high-negative medicine residue fertilizer are added and uniformly mixed, and then 1.5 portions of high-temperature ferment fungus and probiotics are added and uniformly stirred, so that the raw material of the beta-nicotinamide mononucleotide fertilizer is obtained.
Piling the obtained raw materials of the beta-nicotinamide mononucleotide base fertilizer, adding materials layer by layer during piling, adding a beta-nicotinamide mononucleotide aqueous solution with the concentration of 3 while adding materials, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55% -60% of the total mass of the beta-nicotinamide mononucleotide base fertilizer, inserting 4 porous stainless steel pipes into compost after piling, covering a plastic film for sealing, opening a sealing film after 7 days, and turning the pile once by a pile turning machine. Turning the pile once in 7 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the beta-nicotinamide mononucleotide functional nutrient solution in the embodiment is as follows: the weight portions are as follows: dissolving 75 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for standby; and adding 5 parts of yin-yang-essence life mineral fertilizer and 20 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, and finally placing the beta-nicotinamide mononucleotide functional nutrient solution into 4-8 ℃ for storage for standby, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of 30-50 ppm according to the requirement.
The preparation method of the vital essence-yin-yang-essence life mineral fertilizer in the embodiment comprises the following steps: each kg contains 10-15 mg of refined iron, 0.15 mg of refined manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water through high temperature and high pressure, so that the water-soluble mineral fertilizer can be obtained. The vitality elements belong to immune elements in medicine, wherein elements such as yang refined manganese zinc molybdenum selenium and yin refined iron play roles in catalyzing and exciting life cells of the tea, and promote the metabolic function of biological enzymes of the tea.
In this embodiment, the diatom water is the prior art, that is, the water body growing with diatom is the diatom water.
The report of the content of the beta-nicotinamide mononucleotide in the Longjing tea planted in the embodiment is shown in figure 1, and the report of the detection in figure 1 shows that the content of the beta-nicotinamide mononucleotide in each kilogram of tea is 2.01 x 10 4 Mug shows that the planting method of the invention is effective and can successfully obtain tea rich in beta-nicotinamide mononucleotide.
Example 2
A planting method of tea rich in beta-nicotinamide mononucleotide comprises the following planting steps:
(1) Tea tree and planting field selection: the Xinyang hair tips are selected and planted at the altitude of 400-2000 m, the gradient is positive, the pH value is acidic, the land area is more than 200 mu, and the climate is mild.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the holes are 15-20 cm away from tea trees, the depth of the holes is 40 cm, the fertilizer amount is 30Kg per mu, then 60Kg of base fertilizer containing beta-nicotinamide mononucleotide is applied to the tea tree soil every 15 days and covered by soil two months before tea tree picking, and diatom water is poured once at the same time, the spraying amount of the diatom water is 800 liters per mu, so that the tea tree soil is kept moist, and the root absorption of the tea tree is facilitated; and the beta-nicotinamide mononucleotide base fertilizer is applied for the last time 20-30 days before harvest, the application amount of the beta-nicotinamide mononucleotide base fertilizer is 60Kg per mu, meanwhile, diatom water is poured, and the spraying amount of the diatom water is 800 liters per mu.
(3) Pouring diatom water to the tea trees 6 days before picking, wherein the spraying amount of the diatom water is 800 liters per mu;
(4) Spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the first time 13-15 days before tea leaf picking; spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the second time 3-5 days before tea leaf picking, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 30 ppm; the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 40 ppm, and the spraying amount of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 60kg per mu.
The preparation method of the mononucleotide base fertilizer containing the beta-nicotinamide comprises the following steps:
according to the parts by weight, crushing 30 parts of Sang Huangfei fungus sticks, 10 parts of mushroom waste fungus sticks and 5 parts of grifola frondosa waste fungus sticks, adding 25 parts of animal manure and 0.5 part of traditional Chinese medicine high-positive and high-negative medicine residue fertilizer, uniformly mixing, and then adding 1.5 parts of high-temperature ferment fungus and probiotics, and uniformly stirring to obtain the raw material of the beta-nicotinamide mononucleotide fertilizer.
Piling the obtained raw materials of the beta-nicotinamide mononucleotide base fertilizer, adding materials layer by layer during piling, adding a beta-nicotinamide mononucleotide aqueous solution with the concentration of ppm1 while adding materials, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55% -60% of the total mass of the beta-nicotinamide mononucleotide base fertilizer, inserting 4 porous stainless steel pipes into compost after piling, covering a plastic film for sealing, opening a sealing film after 7 days, and turning the pile once by a pile turning machine. Turning the pile once in 6 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the beta-nicotinamide mononucleotide functional nutrient solution in the embodiment is as follows: the weight portions are as follows: dissolving 70 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for standby; and adding 3 parts of yin-yang-essence life mineral fertilizer and 12 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, and finally placing the beta-nicotinamide mononucleotide functional nutrient solution into 4-8 ℃ for storage for standby, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of 30-50 ppm according to the requirement.
The preparation method of the vital essence-yin-yang-essence life mineral fertilizer in the embodiment comprises the following steps: each kg contains 10-15 mg of refined iron, 0.15 mg of refined manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water through high temperature and high pressure, so that the water-soluble mineral fertilizer can be obtained. The vitality elements belong to immune elements in medicine, wherein elements such as yang refined manganese zinc molybdenum selenium and yin refined iron play roles in catalyzing and exciting life cells of the tea, and promote the metabolic function of biological enzymes of the tea.
In this embodiment, the diatom water is the prior art, that is, the water body growing with diatom is the diatom water.
Example 3
(1) Tea tree and planting field selection: pu' er is selected, planted in the land with the altitude of 400-2000 m, positive in gradient, acidic in pH value, 200 mu above the land, and moderate in climate.
(2) Application of tea tree beta-Nicotinamide Mononucleotide (NMN) base fertilizer: digging holes in the soil in winter, wherein the holes are 15-20 cm away from tea trees, the depth of the holes is 40 cm, organic beta-nicotinamide mononucleotide base fertilizer is applied, the fertilizer amount is 40Kg per mu, then 80Kg of beta-nicotinamide mononucleotide base fertilizer is applied to the tea tree soil every 20 days before tea tree picking and covered by soil, diatom water is poured once, the spraying amount of the diatom water is 1200 liters per mu, the tea tree soil is kept moist, and the tea tree root absorption is facilitated; the beta-nicotinamide mononucleotide base fertilizer is applied for the last time 20-30 days before picking, the application amount of the beta-nicotinamide mononucleotide base fertilizer is 80Kg per mu, diatom water is poured at the same time, and the spraying amount of the diatom water is 1200 liters per mu;
(3) The diatom water is poured into the tea trees 10 days before harvest, and the spraying amount of the diatom water is 1200 liters per mu;
(4) Spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the first time 13-15 days before tea leaf picking; spraying the beta-nicotinamide mononucleotide functional nutrient solution to the tea leaves for the second time 3-5 days before tea leaf picking, wherein the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 40 ppm; the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 50 ppm, and the spraying amount of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 60kg per mu.
The preparation method of the mononucleotide base fertilizer containing the beta-nicotinamide comprises the following steps:
according to the parts by weight, crushing 40 parts of Sang Huangfei fungus sticks, 20 parts of mushroom waste fungus sticks and 10 parts of grifola frondosa waste fungus sticks, adding 28 parts of animal manure and 0.5 part of traditional Chinese medicine high-positive and high-negative medicine residue fertilizer, uniformly mixing, and then adding 1.5 parts of high-temperature ferment fungus and probiotics, and uniformly stirring to obtain the raw material of the beta-nicotinamide mononucleotide fertilizer.
Piling the obtained raw materials of the beta-nicotinamide mononucleotide base fertilizer, adding a layer by layer during piling, adding a beta-nicotinamide mononucleotide aqueous solution with the concentration of 5 ppm while adding the raw materials, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55% -60% of the total mass of the beta-nicotinamide mononucleotide base fertilizer, inserting 4 porous stainless steel pipes into compost after piling, covering a plastic film for sealing, opening a sealing film after 7 days, and turning the pile once by a pile turning machine. Turning the pile once in 8 days, and repeating for 4 times to prepare the beta-Nicotinamide Mononucleotide (NMN) base fertilizer.
The preparation method of the beta-nicotinamide mononucleotide functional nutrient solution in the embodiment is as follows: the weight portions are as follows: dissolving 80 parts of beta-nicotinamide mononucleotide by adopting warm water at 50-60 ℃, and standing for 10-15 minutes for standby; and then adding 8 parts of yin-yang-essence life mineral fertilizer and 27 parts of diatom water, uniformly mixing to obtain the beta-nicotinamide mononucleotide functional nutrient solution, and finally placing the beta-nicotinamide mononucleotide functional nutrient solution into 4-8 ℃ for storage for standby, and preparing the beta-nicotinamide mononucleotide functional nutrient solution with the concentration of 30-50 ppm according to the requirement.
The preparation method of the vital essence-yin-yang-essence life mineral fertilizer in the embodiment comprises the following steps: each kg contains 10-15 mg of refined iron, 0.15 mg of refined manganese, 30-40 mg of zinc, 0.05-0.01 mg of selenium and 0.004-0.002 mg of molybdenum; the biological elements are put into a closed mixing tank, and metal ions are dissolved in water through high temperature and high pressure, so that the water-soluble mineral fertilizer can be obtained. The vitality elements belong to immune elements in medicine, wherein elements such as yang refined manganese zinc molybdenum selenium and yin refined iron play roles in catalyzing and exciting life cells of the tea, and promote the metabolic function of biological enzymes of the tea.
In this embodiment, the diatom water is the prior art, that is, the water body growing with diatom is the diatom water.
While the invention has been described with respect to the preferred embodiments, it will be understood that the invention is not limited thereto, but is capable of modification and variation without departing from the spirit of the invention, as will be apparent to those skilled in the art.
Claims (3)
1. A method for planting tea trees rich in beta-nicotinamide mononucleotide, which is characterized by comprising the following steps:
(1) Selecting proper tea trees according to the characteristic of nutrient absorption of the root parts and leaves of the tea trees, and planting the proper tea trees on proper lands;
(2) After digging holes in winter, applying a certain amount of beta-nicotinamide mononucleotide base fertilizer to tea trees; then, supplementing beta-nicotinamide mononucleotide base fertilizer to the tea tree soil every 15-20 days two months before picking the tea tree, and simultaneously watering diatom water; the base fertilizer containing the beta-nicotinamide mononucleotide is applied for the last time 20-30 days before picking, and diatom water is poured at the same time;
(3) Pouring diatom water to the tea tree 6-10 days before harvest;
(4) Spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the first time 13-15 days before tea leaf picking; spraying beta-nicotinamide mononucleotide functional nutrient solution on tea leaves for the second time 3-5 days before tea leaf picking;
in the step (2), the application amount of the beta-nicotinamide mononucleotide base fertilizer after digging holes in winter is 30-40Kg per mu; supplementing and applying beta-nicotinamide mononucleotide base fertilizer two months before spring picking, and supplementing and applying 60-80Kg of beta-nicotinamide mononucleotide base fertilizer per mu of tea tree soil every 15-20 days;
in the steps (2) and (3), the application amount of diatom water is 800-1200 liters per mu each time;
in the step (4), the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the first time is 30-40 ppm; the concentration of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 40-50 ppm, and the spraying amount of the beta-nicotinamide mononucleotide functional nutrient solution sprayed for the second time is 50-60kg per mu;
in the step (2), the formula of the beta-nicotinamide mononucleotide base fertilizer comprises the following components in percentage by mass: 30-40 parts of Sang Huangfei fungus sticks, 10-20 parts of mushroom waste fungus sticks, 5-10 parts of grifola frondosa waste fungus sticks, 1.5 parts of high-temperature ferment fungus and probiotics, 25-28 parts of animal manure, 0.5 part of traditional Chinese medicine high-positive and high-negative medicine residue fertilizer and 1-5 ppm of beta-nicotinamide mononucleotide aqueous solution, wherein the beta-nicotinamide mononucleotide aqueous solution accounts for 55% -65% of the mass of the total beta-nicotinamide mononucleotide base fertilizer;
the preparation of the beta-nicotinamide mononucleotide base fertilizer comprises the following steps: crushing Sang Huangfei fungus sticks, mushroom waste fungus sticks and grifola frondosa waste fungus sticks, adding animal manure and traditional Chinese medicine high-positive and high-negative medicine residue fertilizers, uniformly mixing, adding high-temperature ferment fungus and probiotics, continuously stirring uniformly to obtain a raw material of the beta-nicotinamide mononucleotide base fertilizer, and stacking the raw materials of the beta-nicotinamide mononucleotide base fertilizer, wherein the raw materials are added layer by layer during stacking, and the beta-nicotinamide mononucleotide aqueous solution is added while being added; after the pile is built, a plurality of ventilation pipes are inserted into the compost, the compost is sealed, the sealed pile is opened and turned over once after 6-8 days, and the beta-nicotinamide mononucleotide base fertilizer can be prepared by repeating for a plurality of times.
2. A method for planting tea leaves rich in beta-nicotinamide mononucleotide tea trees according to claim 1, wherein: in the step (1), the tea tree comprises at least one of green tea, yellow tea, black tea, white tea, black tea and oolong tea; the green tea comprises at least one of Longjing tea, xinyang Maojian tea, biluochun tea, yulu tea and Queen tea; black tea includes Dahongpao tea; the black tea comprises Pu' er; oolong tea includes rock tea and/or cinnamon; the land is selected to have the altitude of 400-2000 m, positive gradient, acidic pH value and mild climate.
3. A tea leaf enriched in β -nicotinamide mononucleotide, characterized in that: the method for planting according to claim 1 or 2, wherein the content of the beta-nicotinamide mononucleotide in the tea rich in the beta-nicotinamide mononucleotide is 5-30mg/Kg.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998288A (en) * | 2007-01-04 | 2007-07-18 | 卢万俊 | Method for breeding wild tea tree |
| CN102599305A (en) * | 2012-03-08 | 2012-07-25 | 刘茜 | Selenium-rich chrysanthemum tea or tea |
| CN105191631A (en) * | 2015-09-24 | 2015-12-30 | 广西百润源农业有限公司 | High-yield planting method for pollution-free tea |
| CA3060371A1 (en) * | 2017-04-28 | 2018-11-01 | Nichia Corporation | Method for increasing amount of phenolic compound in plant |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998288A (en) * | 2007-01-04 | 2007-07-18 | 卢万俊 | Method for breeding wild tea tree |
| CN102599305A (en) * | 2012-03-08 | 2012-07-25 | 刘茜 | Selenium-rich chrysanthemum tea or tea |
| CN105191631A (en) * | 2015-09-24 | 2015-12-30 | 广西百润源农业有限公司 | High-yield planting method for pollution-free tea |
| CA3060371A1 (en) * | 2017-04-28 | 2018-11-01 | Nichia Corporation | Method for increasing amount of phenolic compound in plant |
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