CN108085226A - A kind of production method of organic health fruit wine - Google Patents
A kind of production method of organic health fruit wine Download PDFInfo
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- CN108085226A CN108085226A CN201711478456.9A CN201711478456A CN108085226A CN 108085226 A CN108085226 A CN 108085226A CN 201711478456 A CN201711478456 A CN 201711478456A CN 108085226 A CN108085226 A CN 108085226A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/10—Fertilisers containing plant vitamins or hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The present invention relates to a kind of production methods of organic health fruit wine, by the plants interplanting with expelling parasite insecticidal effect in orchard, orchard is managed according to organic planting requirement, fruit prepares fruit juice after harvesting ripe, it will according to a certain percentage be mixed to get organic health fruit wine with base liquor after concentration of juices.The present invention has the advantages of nutrition, health, safety, non agricultural chemical residuum using fruit juice, biological peptide and base liquor mixture.
Description
Technical field
The present invention relates to a kind of production methods of organic health fruit wine.
Background technology
Bread is the staff of life, strengthens food security work, the health and life security of relation China more than 1,300,000,000, it is necessary to
Grab tightly and tightly.These years, food security situation constantly improves, but there are the problem of it is still many, common people still have very
It is expect, it is necessary to redouble one's efforts, work be run business into particular one reality, it is ensured that people's " safety on the tip of the tongue " more.
White wine taste is pungent, is not the taste for meeting very much modern, and market lacks in good taste and with health role
Wine.
The content of the invention
The technical problem to be solved by the invention is to provide a kind of production method of organic health fruit wine, the present invention has
The advantages of nutrition, health, safety, non agricultural chemical residuum.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
A kind of production method of organic health fruit wine, it is characterised in that follow the steps below：
By the plants interplanting with expelling parasite insecticidal effect in orchard, orchard is managed according to organic planting requirement, picks fruit
Mature fruit prepares fruit juice in garden, by fruit juice, plant peptide and base liquor according to mass ratio 1~80:0.5~30:20~98 allotments, i.e.,
Obtain organic health fruit wine；
The plant with expelling parasite insecticidal effect includes garlic, lavender, fish pelargonium, common nepenthes, shichongcao and folium artemisiae argyi
In one kind or arbitrary proportion it is two or more；
Plant is the plant for having health-care efficacy in the plant peptide；
The base liquor is brewed wine, Spirit, and one kind or arbitrary proportion in assembled alcoholic drinks and edible alcohol are two or more；
The fruit juice preparation follows the steps below：
1) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2) cool down：The fruit of harvesting is reduced into temperature to 0~5 DEG C；
3) squeeze the juice：60~120mg/kg of sulfur dioxide concentration is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained to exist
Nitrogen or carbon dioxide are filled with before pan feeding, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 20~50mg/L, and 5~8 DEG C of temperature adds fining agent, stands
24~48h is precipitated, during which inflated with nitrogen or carbon dioxide removal oxygen；
5) low tempertaure storage：Fruit juice in step 4) is stored at 0~5 DEG C, inflated with nitrogen or carbon dioxide bind anti-oxidation；
The enzyme be two kinds of one kind or arbitrary proportion in cellulase, hemicellulase, pectase and protease with
On；
The fining agent is the two or more of one kind in gelatin, bentonite, PVPP and egg white or arbitrary proportion.
The plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 80~200 mesh, is added in the ultrasonic container for fill water and carries
It takes, at least extracts once, obtain extracting solution, each Extracting temperature is 20~70 DEG C, and extraction time is 10~40min, and extraction pH is
2.0~10, the mass ratio of plant and water is 1:5~15；
B) zymolyte is prepared：Add in the enzyme of its weight 0.2%~2% in the extracting solution prepared to step a), enzymolysis 3~
12h obtains zymolyte；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 70~150 DEG C, keeps 5~20min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
E) plant peptide is prepared：To get plant peptide after liquid plant peptide is freeze-dried；
The separation is centrifugation, press filtration separation and the combination more than one or both of being separated by filtration；
The enzyme is the two or more of one kind in cellulase, hemicellulase, pectase and protease or arbitrary ratio.
Fertilizer used in the orchard is two kinds of one kind or arbitrary proportion in organic fertilizer, organic and inorganic fertilizer and biological organic fertilizer
More than；Pesticide used in the orchard for lime sulfur, Bordeaux mixture, mineral oil, trichoderma harzianum, quinolinone suspending agent, ethyl alcohol,
Soda, China tree alkali, rotenone, Dalmatian chrysanthemum missible oil and one kind in matrine or arbitrary proportion are two or more.
Ecological seaweed biostimulant is added in the organic fertilizer, organic and inorganic fertilizer and biological organic fertilizer；
The ecology seaweed biostimulant is prepared according to following steps：
A seaweed fragment), is prepared：Seaweed after cleaning is crushed, obtains seaweed fragment；
B saccharification seaweed), is prepared：By seaweed fragment and water according to mass ratio 5~30:70~95 are fitted into agitator tank,
1~12h is stirred under the conditions of 50~100 DEG C, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 50~70% to be adjusted to water content with water, is obtained
The mass ratio of mixed culture medium, access candida utili, candida utili and mixed culture medium is 0.2~2:98~
99.8, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 0.1~0.5mg/L, ferments 2~7 days, is 70~150 DEG C in temperature
Under the conditions of sterilize 20~90min, obtain fermentation of seaweed object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 96~99.5:0.5~4, it is 25~35 DEG C in temperature, oxygen content 1
It under the conditions of~5mg/L, ferments 2~7 days, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is sea
Algae residue；
F ecological seaweed biostimulant), is prepared：Ecological seaweed liquid biostimulant it is concentrated and spray drying after, i.e.,
Obtain ecological seaweed biostimulant；
The seaweed is that one kind in Enteromorpha, kelp, sargassum, opotism and kelp or arbitrary proportion are two or more.
The preparation of corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：Thawing method is at least repeated once, and step is：Step 1) is obtained
Expansion is further cultured for strain and is put into -20 DEG C of refrigerator, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Step A) seaweed is Fresh Laminaria Japonica.
The candida utili and corynebacterium ammoniagenes are purchased from Chinese industrial Microbiological Culture Collection administrative center, number
Respectively CICC 31170 and CICC 10168.
Cellulase, hemicellulase, pectase and protease are according to mass ratio 2:1:1:2.
Cellulase, hemicellulase, pectase and protease are the cellulase Validase of Royal DSM company production
TRL, hemicellulase Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP
Concentrate。
The mode that ecological seaweed biostimulant is added in fertilizer is：With the interior addition manner of fertilizer material mixing granulation
With the outer addition manner for spraying to fertiliser granulates surface.
Invention has following advantageous effects：
1st, the present invention uses organic fruits as raw material, and the fruit juice prepared has nutrition, health, safety, non agricultural chemical residuum
The advantages of.
2nd, by anti-oxidation techniques such as fruit juice preparation process whole process inflated with nitrogen or carbon dioxide, fruit juice is increased to greatest extent
Freshness and nutriment activity；The present invention need not add preservative substance.
3rd, the present invention can reach expelling parasite and reduce pesticide by interplanting the plant with expelling parasite insecticidal effect in orchard
The purpose of usage amount.
4th, ecological seaweed biostimulant uses new fresh seaweed in the present invention, and life can largely be stimulated by containing in new fresh seaweed
Long stimulin and resistance substance, activity is good, can achieve the purpose that reduce Fertilizer application amount.
5th, ecological seaweed biostimulant uses aerobic and anaerobism candida utili under anaerobic in the present invention
Fermentation can prevent the loss of seaweed nutritive ingredient, and the alcohol that anaerobic fermentation generates is a supporting role seaweed extraction.
6th, ecological seaweed biostimulant is sent out under anoxic conditions using aerobic and anaerobism corynebacterium ammoniagenes in the present invention
Ferment produces ATP, and ATP is a kind of high energy phosphate compound, and in cell, it can realize and energy storage and put with mutually converting for ADP
Can, so as to ensure that the energy supply of cell items vital movement, promote plant growth.It is unreasonable due to long-term fertilization, it makes
Into soil acidification, corynebacterium ammoniagenes can reduce N03 -And urea, generating ammonia makes tunning that can improve acidifying soil in alkalescent
Earth problem.
7th, ecological seaweed biostimulant has heavy metal certain fixation in the present invention.Contain in fermentate of the present invention
There are polypeptide, alginic acid and other protides, heavy metal can make albumen, alginic acid and polypeptide inactivation, while heavy metal also can
It is fixed by albumen, alginic acid and polypeptide, so as to prevent crop from absorbing.
8th, the present invention is using aerobic and oxygen bacterium, and soil hardening is serious at present, and the microorganism applied in ground is often in anoxic shape
State is suitable for present soil environment using aerobic and oxygen bacterium.
9th, ecological seaweed biostimulant improves the permeability of corynebacterium ammoniagenes cell by thawing method in the present invention, can be with
The ATP that corynebacterium ammoniagenes generate is made to be easier to penetrate into from cell membrane extracellularly, product effects are significantly better than with without place
The product of the corynebacterium ammoniagenes of reason.
Specific embodiment
It is further illustrated the present invention with reference to specific example.
Embodiment 1
By fish pelargonium interplanting in orchard, orchard is managed according to organic planting requirement, mature fruit in orchard is picked and prepares
Fruit juice picks ripe grape in orchard and carries out vintage wine, by fruit juice, plant peptide and brewed wine according to mass ratio 5:1:94 adjust
With to get organic health fruit wine；
The fruit juice preparation follows the steps below：
1) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2) cool down：The fruit of harvesting is reduced into temperature to 3 DEG C；
3) squeeze the juice：Sulfur dioxide concentration 45mg/kg is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained before pan feeding
Nitrogen is filled with, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 40mg/L, and 6 DEG C of temperature is added fining agent, staticly settled
For 24 hours, during which inflated with nitrogen removal oxygen；
5) low tempertaure storage：Fruit juice is stored at 2 DEG C, inflated with nitrogen binds anti-oxidation；
The fruit is strawberry；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:1:1:2；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
The fining agent is gelatin；
The plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 100 mesh, is added in the ultrasonic container for fill water and extracts 2
It is secondary, extracting solution is obtained, each Extracting temperature is 50 DEG C, extraction time 20min, and extraction pH is 8, and the mass ratio of plant and water is 1:
8；
B) zymolyte is prepared：The enzyme of its weight 0.4% is added in the extracting solution prepared to step a), 6h is digested, must digest
Object；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 120 DEG C, keeps 10min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
E) plant peptide is prepared：To get plant peptide after liquid plant peptide is freeze-dried；
Plant described in plant peptide is maca；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:1:1:2；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
The orchard uses the organic fertilizer for adding in ecological seaweed biostimulant；
The ecology seaweed biostimulant is prepared according to following steps：
A kelp fragment), is prepared：Fresh Laminaria Japonica after cleaning is dried and then crushed, obtains kelp fragment；
B saccharification seaweed), is prepared：By kelp fragment and water according to mass ratio 20:80 are fitted into agitator tank, in 60 DEG C of items
3h is stirred under part, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 60% to be adjusted to water content with water, must be mixed
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.6:99.4, it is 32 in temperature
DEG C, it under the conditions of oxygen content is 0.1~0.5mg/L, ferments 5 days, sterilize 40min under the conditions of temperature is 120 DEG C, obtains fermentation of seaweed
Object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 98:2, it is 32 DEG C in temperature, under the conditions of oxygen content is 2mg/L, fermentation 6
My god, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
F ecological seaweed biostimulant), is prepared：Ecological seaweed liquid biostimulant it is concentrated and spray drying after, i.e.,
Obtain ecological seaweed biostimulant；
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：The repetition of thawing method is secondary, and step is：The expansion that step 1) is obtained
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, it is cold
Freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 2
By fish pelargonium interplanting in orchard, orchard is managed according to organic planting requirement, mature fruit in orchard is picked and prepares
Fruit juice, by fruit juice, plant peptide and Spirit according to mass ratio 10:5:85 allotments are to get organic health fruit wine；
The fruit juice preparation follows the steps below：
1) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2) cool down：The fruit of harvesting is reduced into temperature to 3 DEG C；
3) squeeze the juice：Sulfur dioxide concentration 80mg/kg is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained before pan feeding
Nitrogen is filled with, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 40mg/L, and 6 DEG C of temperature is added fining agent, staticly settled
For 24 hours, during which inflated with nitrogen removal oxygen；
5) low tempertaure storage：Fruit juice is stored at 2 DEG C, inflated with nitrogen binds anti-oxidation；
The fruit is strawberry；
The enzyme is pectase；
The fining agent is gelatin.
The plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 80 mesh, is added in the ultrasonic container for fill water and extracts one
It is secondary, extracting solution is obtained, Extracting temperature is 60 DEG C, extraction time 20min, and extraction pH is 4, and the mass ratio of plant and water is 1:7；
B) zymolyte is prepared：The enzyme of its weight 1% is added in the extracting solution prepared to step a), 4h is digested, obtains zymolyte；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 90 DEG C, keeps 10min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
E) plant peptide is prepared：To get plant peptide after liquid plant peptide is freeze-dried；
Plant described in plant peptide is ginseng；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 1:2:2:1；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
The orchard uses the organic fertilizer for adding in ecological seaweed biostimulant；
The ecology seaweed biostimulant is prepared according to following steps：
A kelp fragment), is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains kelp fragment；
B saccharification seaweed), is prepared：By kelp fragment and water according to mass ratio 20:80 are fitted into agitator tank, in 60 DEG C of items
3h is stirred under part, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 60% to be adjusted to water content with water, must be mixed
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.6:99.4, it is 32 in temperature
DEG C, it under the conditions of oxygen content is 0.1~0.5mg/L, ferments 5 days, sterilize 40min under the conditions of temperature is 120 DEG C, obtains fermentation of seaweed
Object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 98:2, it is 32 DEG C in temperature, under the conditions of oxygen content is 2mg/L, fermentation 6
My god, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
F ecological seaweed biostimulant), is prepared：Ecological seaweed liquid biostimulant it is concentrated and spray drying after, i.e.,
Obtain ecological seaweed biostimulant；
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：The repetition of thawing method is secondary, and step is：The expansion that step 1) is obtained
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, it is cold
Freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 3
By fish pelargonium interplanting in orchard, orchard is managed according to organic planting requirement, mature fruit in orchard is picked and prepares
Fruit juice, by fruit juice, plant peptide and 60 degree of edible alcohols according to mass ratio 30:5:5:60 allotments are to get organic health fruit wine；
The fruit juice preparation follows the steps below：
1) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2) cool down：The fruit of harvesting is reduced into temperature to 3 DEG C；
3) squeeze the juice：Sulfur dioxide concentration 80mg/kg is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained before pan feeding
Nitrogen is filled with, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 40mg/L, and 6 DEG C of temperature is added fining agent, staticly settled
For 24 hours, during which inflated with nitrogen removal oxygen；
5) low tempertaure storage：Fruit juice is stored at 2 DEG C, inflated with nitrogen binds anti-oxidation；
The fruit is cherry；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:2:1:1；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
The fining agent is gelatin and PVPP according to mass ratio 3:1 composition.
The plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 150 mesh, is added in the ultrasonic container for fill water and extracts 3
It is secondary, extracting solution is obtained, each Extracting temperature is 30 DEG C, extraction time 15min, and extraction pH is 5, and the mass ratio of plant and water is 1:
12；
B) zymolyte is prepared：The enzyme of its weight 0.8% is added in the extracting solution prepared to step a), 10h is digested, must digest
Object；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 100 DEG C, keeps 10min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
E) plant peptide is prepared：To get plant peptide after liquid plant peptide is freeze-dried；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:1:1:2；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
Plant described in plant peptide is matrimony vine and the fleece-flower root according to mass ratio 1:2 composition；
The organic fertilizer that the orchard is prepared using seaweed residue；
The seaweed residue is prepared according to following steps：
A seaweed fragment), is prepared：Fresh kelp after cleaning is crushed, obtains seaweed fragment；
B saccharification seaweed), is prepared：By seaweed fragment and water according to mass ratio 20:80 are fitted into agitator tank, in 60 DEG C of items
3h is stirred under part, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 60% to be adjusted to water content with water, must be mixed
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.6:99.4, it is 32 in temperature
DEG C, it under the conditions of oxygen content is 0.1~0.5mg/L, ferments 5 days, sterilize 40min under the conditions of temperature is 120 DEG C, obtains fermentation of seaweed
Object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 98:2, it is 32 DEG C in temperature, under the conditions of oxygen content is 2mg/L, fermentation 6
My god, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：The repetition of thawing method is secondary, and step is：The expansion that step 1) is obtained
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, it is cold
Freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 4
By fish pelargonium interplanting in orchard, orchard is managed according to organic planting requirement, mature fruit in orchard is picked and prepares
Fruit juice, by fruit juice, liquid plant peptide and allotment wine according to mass ratio 60:20:20 allotments are to get organic health fruit wine；The tune
With wine by 70 degree of 70 parts of brewed wines, 5 parts of honey, 1 part of edible glycerol mixes；
The fruit juice preparation follows the steps below：
1) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2) cool down：The fruit of harvesting is reduced into temperature to 3 DEG C；
3) squeeze the juice：Sulfur dioxide concentration 80mg/kg is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained before pan feeding
Nitrogen is filled with, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 40mg/L, and 6 DEG C of temperature is added fining agent, staticly settled
For 24 hours, during which inflated with nitrogen removal oxygen；
5) low tempertaure storage：Fruit juice is stored at 2 DEG C, inflated with nitrogen binds anti-oxidation；
The fruit is cherry；
The enzyme is pectase and cellulase according to mass ratio 3:1 composition；
The fining agent is gelatin and PVPP according to mass ratio 1:1 composition.
The liquid plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 100 mesh, is added in the ultrasonic container for fill water and extracts 2
It is secondary, extracting solution is obtained, each Extracting temperature is 40 DEG C, extraction time 20min, and extraction pH is 9, and the mass ratio of plant and water is 1:
10；
B) zymolyte is prepared：The enzyme of its weight 1.5% is added in the extracting solution prepared to step a), 8h is digested, must digest
Object；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 80 DEG C, keeps 20min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
Plant described in plant peptide is Huang Shi；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 1:1:2:2；Cellulase,
Cellulase Validase TRL, the hemicellulase of hemicellulase, pectase and protease for the production of Royal DSM company
Bekezyme HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
The organic fertilizer that the orchard is prepared using seaweed residue；
The seaweed residue is prepared according to following steps：
A seaweed fragment), is prepared：Fresh kelp after cleaning is crushed, obtains seaweed fragment；
B saccharification seaweed), is prepared：By seaweed fragment and water according to mass ratio 20:80 are fitted into agitator tank, in 60 DEG C of items
3h is stirred under part, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 60% to be adjusted to water content with water, must be mixed
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.6:99.4, it is 32 in temperature
DEG C, it under the conditions of oxygen content is 0.1~0.5mg/L, ferments 5 days, sterilize 40min under the conditions of temperature is 120 DEG C, obtains fermentation of seaweed
Object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 98:2, it is 32 DEG C in temperature, under the conditions of oxygen content is 2mg/L, fermentation 6
My god, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：The repetition of thawing method is secondary, and step is：The expansion that step 1) is obtained
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, it is cold
Freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 5
By fish pelargonium interplanting in orchard, orchard is managed according to organic planting requirement, mature fruit in orchard is picked and prepares
Fruit juice, by fruit juice, plant peptide and brewed wine according to mass ratio 5:1:94 allotments are to get organic health fruit wine；
The fruit juice preparation follows the steps below：
4) harvest：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
5) cool down：The fruit of harvesting is reduced into temperature to 3 DEG C；
6) squeeze the juice：Sulfur dioxide concentration 45mg/kg is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained before pan feeding
Nitrogen is filled with, air-discharging is anti-oxidation；
4) clarify：Enzyme is added in fruit juice, enzyme addition is 40mg/L, and 6 DEG C of temperature is added fining agent, staticly settled
For 24 hours, during which inflated with nitrogen removal oxygen；
5) low tempertaure storage：Fruit juice is stored at 2 DEG C, inflated with nitrogen binds anti-oxidation；
The fruit is strawberry；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:1:1:2.
The fining agent is gelatin.
The plant peptide follows the steps below：
A) ultrasonic extraction：Plant after drying is crushed to 100 mesh, is added in the ultrasonic container for fill water and extracts 2
It is secondary, extracting solution is obtained, each Extracting temperature is 50 DEG C, extraction time 20min, and extraction pH is 8, and the mass ratio of plant and water is 1:
8；
B) zymolyte is prepared：The enzyme of its weight 0.4% is added in the extracting solution prepared to step a), 6h is digested, must digest
Object；
C) enzyme deactivation：Zymolyte prepared by step b) is heated to 120 DEG C, keeps 10min, enzyme deactivation；
D) liquid plant peptide is prepared：Product after separating step c) enzyme deactivations obtains clarified solution, and clarified solution is by using 0.45 He
0.20 micron of film device degerming, obtains liquid plant peptide；
E) plant peptide is prepared：To get plant peptide after liquid plant peptide is freeze-dried；
Plant described in plant peptide is maca；
The enzyme is cellulase, hemicellulase, pectase and protease according to mass ratio 2:1:1:2.
The orchard uses the organic fertilizer for adding in ecological seaweed biostimulant；
The ecology seaweed biostimulant is prepared according to following steps：
A kelp fragment), is prepared：Fresh Laminaria Japonica after cleaning is dried and then crushed, obtains kelp fragment；
B saccharification seaweed), is prepared：By kelp fragment and water according to mass ratio 20:80 are fitted into agitator tank, in 60 DEG C of items
3h is stirred under part, must be saccharified seaweed；
C seaweed filtrate and seaweed slag), are prepared：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, residue obtained to be
Seaweed slag；
D fermentation of seaweed object), is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 60% to be adjusted to water content with water, must be mixed
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.6:99.4, it is 32 in temperature
DEG C, it under the conditions of oxygen content is 0.1~0.5mg/L, ferments 5 days, sterilize 40min under the conditions of temperature is 120 DEG C, obtains fermentation of seaweed
Object；
E ecological seaweed liquid biostimulant and seaweed residue), are prepared：The seaweed filtrate that step 3) obtains is added to
It is stirred and evenly mixed in the fermentation of seaweed object that step 4) obtains, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture
The mass ratio of the corynebacterium ammoniagenes prepared with thawing method is 98:2, it is 32 DEG C in temperature, under the conditions of oxygen content is 2mg/L, fermentation 6
My god, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
F ecological seaweed biostimulant), is prepared：Ecological seaweed liquid biostimulant it is concentrated and spray drying after, i.e.,
Obtain ecological seaweed biostimulant；
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
I) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
II) thawing method improve cell permeability：The repetition of thawing method is secondary, and step is：The expansion that step 1) is obtained
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, it is cold
Freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
The advantageous effect further illustrated the present invention with reference to experimental data：
Experiment one
1st, material to be tested is tested
1 materials and methods：
1.1 test site：Laboratory.
1.2 material to be tested：Strawberry, comparison 1 in common orchard (non-organic orchard, using inorganic fertilizer and pesticide) (remove
Do not added in the organic fertilizer used outside ecological seaweed biostimulant, it is other processing it is consistent with the present invention), comparison 2 (except prepare
During ecological seaweed biostimulant, corynebacterium ammoniagenes are outer without the processing of thawing method, and other preparation methods are consistent with the present invention) and
Strawberry in the organic orchard of the present invention, compares.
This experiment is consistent with other management in addition to processing difference except testing, and strawberry cultivars are sweet tea Charlie.
1.3 experimental design：The strawberry in common orchard (non-organic orchard), comparison 1 are picked respectively (except the organic fertilizer used
In do not add in outside ecological seaweed biostimulant, other processing are consistent with the present invention), comparison 2 is (except preparing ecological seaweed bio
During stimulin, corynebacterium ammoniagenes are outer without the processing of thawing method, and other preparation methods are consistent with the present invention) and the organic fruit of the present invention
Strawberry detection NO in garden2 -, pesticide residue and sugar content.
2 results and analysis in table 1
Table 1
Though by data compare it can be seen from using the common orchard yield of fertilizer and pesticide higher than the present invention, it exists
NO2 -, Pesticide Residue, and sugar content is low, and mouthfeel is not good enough；Comparison 1 is compared with the present invention, it can be seen that product of the invention
Comparison 1 is above with sugar content, illustrates that plant growth can be stimulated and improve crop quality by adding in ecological seaweed biostimulant；
Compared by comparison 2 and data of the present invention as can be seen that of the invention be superior to comparison 2 in terms of yield and sugar content, illustrate production ammonia
For bar bacterium after the processing of thawing method, effect is more prominent.
Experiment two
1st, material to be tested is tested
1 materials and methods：
1.1 test site：Laboratory.
1.2 material to be tested：Plant chooses maca in plant peptide, is prepared and planted using the preparation method of embodiment 1 to embodiment 5
Object peptide and comparison 1 (except the enzyme of addition is in addition to compound protease in plant peptide preparation process, other preparation methods with embodiment 1
Unanimously) the plant peptide prepared, compares.
This experiment is consistent with other experiments in addition to processing difference except testing.
1.3 experimental design：According to national standard《The measure of amino acid in food》Method makes embodiment 1 to embodiment 5
Standby plant peptide and comparison 1 (in plant peptide preparation process in addition to the enzyme of addition is compound protease, other preparation methods with reality
It is consistent to apply example 1) the plant peptide detection total amino acid content for preparing.
2 results and analysis in table 2
Table 2
| Total amino acid content (%) | |
| Embodiment 1 | 30.27 |
| Embodiment 2 | 28.54 |
| Embodiment 3 | 29.32 |
| Embodiment 4 | 28.77 |
| Embodiment 5 | 29.37 |
| Comparison 1 | 25.56 |
Compared by embodiment 1 to 4 data of embodiment it can be seen that the ratio for the enzyme that embodiment is selected is optimal；By embodiment 1
Compare with 5 data of embodiment it can be seen that the enzyme effect of Royal DSM company production is selected to be significantly better than the enzyme of common producer production；
Compared by embodiment 1 and 1 data of comparison as can be seen that being compounded using cellulase, hemicellulase, pectase and protease
Amino acid is with obvious effects is better than using only compound protease for enzyme extraction.
Experiment three
1st, general information
Inventor selects 300 volunteers to use products of the present invention between in July, 2016~2017 year July, wherein male
Property, each 150 of women, the range of age be 40~60 years old.Universal symptom is weakness of limbs with dizziness and nausea symptom.By this 300
Name experimenter is divided into 3 groups immediately, and every group of 100 people, the fruit wine prepared respectively according to 1 preparation method of embodiment is made according to embodiment 5
Fruit wine prepared by fruit wine and comparison 1 prepared by Preparation Method (except the enzyme of addition is in addition to compound protease in plant peptide preparation process,
Its preparation method is consistent with embodiment 1).
Embodiment 1, embodiment 5 and fruit selected by comparison 1 are Kiwi berry, and plant selected by plant peptide is Huang Shi.
2nd, test method
Sooner or later respectively drink once, each 25ml continuously takes 3 months.
3rd, judgment criteria and evaluation result
3.1 judgment criteria
It is divided into recovery from illness：Energetic, dizziness and nausea symptom disappears；It improves：Weakness of limbs phenomenon mitigates, dizziness and nausea part
Symptom disappears；It is invalid：It is unchanged；Adverse reaction：Exacerbation of symptoms.
3.2 evaluation results statistics such as table 3.
Table 3
| It fully recovers (%) | It improves (%) | Invalid (%) | Adverse reaction (%) | |
| Embodiment 1 | 43 | 28 | 29 | 0 |
| Embodiment 5 | 32 | 17 | 51 | 0 |
| Comparison 1 | 23 | 12 | 65 | 0 |
It can be seen that the present invention that the enzyme of Royal DSM company production is selected to prepare by embodiment 1 and 5 data comparison of embodiment
Product prepared by the enzyme with obvious effects for being better than common producer's production of product；It can relatively be seen by embodiment 1 and 1 data of comparison
Go out, using cellulase, hemicellulase, pectase and protease compounding enzyme prepare product of the present invention it is with obvious effects good
In the product effect prepared using only compound protease.
Separately there is hyperpietic, after taking the present invention, blood pressure is declined.
It is high so-and-so, Shandong people from Qixia original blood pressure 90/140 takes this product and blood pressure 85/130 is detected after 3 months.
" managing orchard according to organic planting requirement " of the present invention refers to according to GB/T19630《Organic products》Mark
Quasi- management orchard.
Claims (10)
1. a kind of production method of organic health fruit wine, it is characterised in that follow the steps below：
By the plants interplanting with expelling parasite insecticidal effect in orchard, orchard is managed according to organic planting requirement, is picked in orchard
Mature fruit prepares fruit juice, by fruit juice, plant peptide and base liquor according to mass ratio 1~80:0.5~30:20~98 allocate to have
Machine health fruit；
The plant with expelling parasite insecticidal effect is included in garlic, lavender, fish pelargonium, common nepenthes, shichongcao and folium artemisiae argyi
A kind of or arbitrary proportion is two or more；
Plant is the plant for having health-care efficacy in the plant peptide；
The base liquor is brewed wine, Spirit, and one kind or arbitrary proportion in assembled alcoholic drinks and edible alcohol are two or more；
The fruit juice preparation follows the steps below：
1）Harvesting：Organic orchard harvests fresh, healthy, disease-free rotten fruit；
2）Cooling：The fruit of harvesting is reduced into temperature to 0~5 DEG C；
3）It squeezes the juice：60~120mg/kg of sulfur dioxide concentration is added in fruit, flexibility is broken, squeezes the juice；Juice container is contained in pan feeding
Before be filled with nitrogen or carbon dioxide, air-discharging is anti-oxidation；
4）Clarification：Enzyme is added in fruit juice, enzyme addition is 20~50mg/L, and 5~8 DEG C of temperature is added fining agent, staticly settled
24~48h, during which inflated with nitrogen or carbon dioxide remove oxygen；
5）Low tempertaure storage：By step 4）In fruit juice stored at 0~5 DEG C, inflated with nitrogen or carbon dioxide bind anti-oxidation；
The enzyme is the two or more of one kind in cellulase, hemicellulase, pectase and protease or arbitrary proportion；
The fining agent is the two or more of one kind in gelatin, bentonite, PVPP and egg white or arbitrary proportion.
2. the production method of organic health fruit wine as described in claim 1, it is characterised in that：The plant peptide is according to following steps
It carries out：
a）Ultrasonic extraction：Plant after drying is crushed to 80~200 mesh, is added in the ultrasonic container for fill water and extracts,
It at least extracts once, obtains extracting solution, each Extracting temperature is 20~70 DEG C, and extraction time is 10~40min, and extraction pH is 2.0
~10, the mass ratio of plant and water is 1:5~15；
b）Prepare zymolyte：To step a）The enzyme of its weight 0.2%~2% is added in the extracting solution of preparation, 3~12h is digested, obtains enzyme
Solve object；
c）Enzyme deactivation：Zymolyte prepared by step b) is heated to 70~150 DEG C, keeps 5~20min, enzyme deactivation；
d）Prepare liquid plant peptide：Separating step c）Product after enzyme deactivation obtains clarified solution, and clarified solution is by using 0.45 and 0.20
Micron film device degerming, obtains liquid plant peptide；
e）Prepare plant peptide：To get plant peptide after liquid plant peptide is freeze-dried；
The separation is centrifugation, press filtration separation and the combination more than one or both of being separated by filtration；
The enzyme is the two or more of one kind in cellulase, hemicellulase, pectase and protease or arbitrary ratio.
3. the production method of organic health fruit wine as described in claim 1, it is characterised in that：Fertilizer used in the orchard is organic
Fertilizer, organic and inorganic fertilizer and one kind in biological organic fertilizer or arbitrary proportion are two or more；Pesticide used in the orchard closes for stone sulphur
Agent, Bordeaux mixture, mineral oil, trichoderma harzianum, quinolinone suspending agent, ethyl alcohol, soda, China tree alkali, rotenone, Dalmatian chrysanthemum missible oil
It is two or more with one kind in matrine or arbitrary proportion.
4. the production method of organic health fruit wine as claimed in claim 3, it is characterised in that：The organic fertilizer, organic and inorganic fertilizer
With the ecological seaweed biostimulant of addition in biological organic fertilizer；
The ecology seaweed biostimulant is prepared according to following steps：
A）, prepare seaweed fragment：Seaweed after cleaning is crushed, obtains seaweed fragment；
B）, prepare saccharification seaweed：By seaweed fragment and water according to mass ratio 5~30:70~95 are fitted into agitator tank, 50~
1~12h is stirred under the conditions of 100 DEG C, must be saccharified seaweed；
C）, prepare seaweed filtrate and seaweed slag：To be saccharified seaweed press filtration, and gained filtrate is seaweed filtrate, and residue obtained is seaweed
Slag；
D）, prepare fermentation of seaweed object：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to quality
Than 95.5:1:0.5:1:1:1 is added to mixing in the fermentation tank to sterilize, and it is 50~70% to be adjusted to water content with water, must mix training
The mass ratio of foster base, access candida utili, candida utili and mixed culture medium is 0.2~2:98~99.8, in temperature
It spends for 25~35 DEG C, under the conditions of oxygen content is 0.1~0.5mg/L, ferments 2~7 days, go out under the conditions of temperature is 70~150 DEG C
20~90min of bacterium obtains fermentation of seaweed object；
E）, prepare ecological seaweed liquid biostimulant and seaweed residue：By step 3）Obtained seaweed filtrate is added to step
4）It is stirred and evenly mixed in obtained fermentation of seaweed object, obtains mixture, corynebacterium ammoniagenes prepared by access thawing method, mixture and thawing
The mass ratio of corynebacterium ammoniagenes prepared by method is 96~99.5:0.5~4, it is 25~35 DEG C in temperature, oxygen content is 1~5mg/L
Under the conditions of, it ferments 2~7 days, gained filtrate is ecological seaweed liquid biostimulant after press filtration；Filter residue is seaweed residue；
F）, prepare ecological seaweed biostimulant：Ecological seaweed liquid biostimulant it is concentrated and spray drying after to get life
State seaweed bio stimulin；
The seaweed is that one kind in Enteromorpha, kelp, sargassum, opotism and kelp or arbitrary proportion are two or more.
5. the production method of organic health fruit wine as claimed in claim 4, it is characterised in that：The preparation of corynebacterium ammoniagenes according to
Lower step carries out：
ⅰ）The expansion of strain is further cultured for：Using the thalline in exponential phase as seed, liquid is inoculated in 10% inoculum concentration
In culture medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for strain；
ⅱ）Thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：By step 1）Obtained expansion
It is further cultured for strain to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, 20.0~25.0g of agar;
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams and is soaked in
It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
6. the production method of organic health fruit wine as claimed in claim 4, it is characterised in that：Step A）The seaweed is fresh
Kelp.
7. the production method of organic health fruit wine as described in claim 4 or 5, it is characterised in that：The candida utili
Chinese industrial Microbiological Culture Collection administrative center is purchased from corynebacterium ammoniagenes, number is respectively CICC 31170 and CICC
10168。
8. the production method of organic health fruit wine as claimed in claim 1 or 2, it is characterised in that：Cellulase, hemicellulose
Enzyme, pectase and protease are according to mass ratio 2:1:1:2.
9. the production method of organic health fruit wine as claimed in claim 8, it is characterised in that：Cellulase, hemicellulase,
Pectase and cellulase Validase TRL, hemicellulase Bekezyme that protease is the production of Royal DSM company
HSP6000BG, pectase Klzrzyme150 and protease ValidaseFP Concentrate.
10. the production method of organic health fruit wine as claimed in claim 4, it is characterised in that：Ecological seaweed biostimulant
The mode being added in fertilizer is：With the interior addition manner of fertilizer material mixing granulation and spraying to the outer of fertiliser granulates surface and adding
Add mode.
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Cited By (2)
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|---|---|---|---|---|
| CN108753532A (en) * | 2018-06-26 | 2018-11-06 | 广西驰胜农业科技有限公司 | A kind of brewage process of holy girl's fruit wine |
| CN109097234A (en) * | 2018-09-05 | 2018-12-28 | 天津农学院 | A kind of production method of allotment type persimmon brandy |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086133A (en) * | 2016-06-20 | 2016-11-09 | 武汉轻工大学 | A kind of enzyme process prepares the method for Semen coryli heterophyllae albumen |
| CN106479839A (en) * | 2016-11-24 | 2017-03-08 | 李多伟 | A kind of eucommia Bark male flower peptide wine and preparation method thereof |
| CN106692425A (en) * | 2016-12-20 | 2017-05-24 | 广州聚澜健康产业研究院有限公司 | Female healthcare wine for fading chloasma, maintaining beauty and keeping young |
| CN106929353A (en) * | 2017-02-14 | 2017-07-07 | 广西肽王生物科技有限公司 | A kind of antidepressant Moringa peptide method for preparing medicated wine |
| CN107418807A (en) * | 2017-09-14 | 2017-12-01 | 吴毅 | A kind of production method of Organic grape fruit wine |
-
2017
- 2017-12-29 CN CN201711478456.9A patent/CN108085226A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086133A (en) * | 2016-06-20 | 2016-11-09 | 武汉轻工大学 | A kind of enzyme process prepares the method for Semen coryli heterophyllae albumen |
| CN106479839A (en) * | 2016-11-24 | 2017-03-08 | 李多伟 | A kind of eucommia Bark male flower peptide wine and preparation method thereof |
| CN106692425A (en) * | 2016-12-20 | 2017-05-24 | 广州聚澜健康产业研究院有限公司 | Female healthcare wine for fading chloasma, maintaining beauty and keeping young |
| CN106929353A (en) * | 2017-02-14 | 2017-07-07 | 广西肽王生物科技有限公司 | A kind of antidepressant Moringa peptide method for preparing medicated wine |
| CN107418807A (en) * | 2017-09-14 | 2017-12-01 | 吴毅 | A kind of production method of Organic grape fruit wine |
Non-Patent Citations (2)
| Title |
|---|
| （英）伯 奇（BIRCH，G.G.）等: "《酶与食品加工》", 31 March 1991, 轻工业出版社 * |
| 黄耀江: "《蛋白质工程原理及应用》", 31 August 2007, 中央民族大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753532A (en) * | 2018-06-26 | 2018-11-06 | 广西驰胜农业科技有限公司 | A kind of brewage process of holy girl's fruit wine |
| CN109097234A (en) * | 2018-09-05 | 2018-12-28 | 天津农学院 | A kind of production method of allotment type persimmon brandy |
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