CN112424349A - 突变的Cpf1核酸内切酶 - Google Patents

突变的Cpf1核酸内切酶 Download PDF

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CN112424349A
CN112424349A CN201980047153.2A CN201980047153A CN112424349A CN 112424349 A CN112424349 A CN 112424349A CN 201980047153 A CN201980047153 A CN 201980047153A CN 112424349 A CN112424349 A CN 112424349A
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seq
nucleic acid
cpf1
target nucleic
sequence
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S·斯特拉
G·蒙托亚
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Københavns Universitet
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201980047153.2A 2018-06-04 2019-06-04 突变的Cpf1核酸内切酶 Pending CN112424349A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP18175707 2018-06-04
EP18175707.1 2018-06-04
EP18190950.8 2018-08-27
EP18190950 2018-08-27
PCT/EP2019/064444 WO2019233990A1 (en) 2018-06-04 2019-06-04 Mutant cpf1 endonucleases

Publications (1)

Publication Number Publication Date
CN112424349A true CN112424349A (zh) 2021-02-26

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CN201980047153.2A Pending CN112424349A (zh) 2018-06-04 2019-06-04 突变的Cpf1核酸内切酶

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US (2) US12152257B2 (https=)
EP (1) EP3802806A1 (https=)
JP (2) JP2021525541A (https=)
KR (1) KR20210028163A (https=)
CN (1) CN112424349A (https=)
AU (1) AU2019282094A1 (https=)
BR (1) BR112020024736A2 (https=)
CA (1) CA3102676A1 (https=)
IL (1) IL279153A (https=)
MA (1) MA52739A (https=)
MX (1) MX2020013229A (https=)
WO (1) WO2019233990A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119506249A (zh) * 2024-11-29 2025-02-25 大连理工大学 一种多PAM选择的高效基因编辑系统NovaCas-1及其应用

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
EP3823633A4 (en) 2018-06-29 2023-05-03 Editas Medicine, Inc. SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO
CN111521781B (zh) * 2020-02-24 2021-07-30 广州医科大学附属第一医院（广州呼吸中心） 一种用于新冠肺炎病毒SARS-CoV-2核酸的检测试剂盒及其检测方法
JP7850156B2 (ja) * 2020-12-17 2026-04-22 モンサント テクノロジー エルエルシー 操作されたｓｓＤＮａｓｅ不含ＣＲＩＳＰＲエンドヌクレアーゼ
EP4284923A1 (en) 2021-01-29 2023-12-06 Wageningen Universiteit Crispr-associated base-editing of the complementary strand
US20250025572A1 (en) * 2021-11-29 2025-01-23 Editas Medicine, Inc. Engineered crispr/cas12a effector proteins, and uses thereof
WO2023166030A1 (en) 2022-03-01 2023-09-07 BASF Agricultural Solutions Seed US LLC Cas12a nickases
JP2025511267A (ja) 2022-03-30 2025-04-15 ビーエーエスエフ アグリカルチュラル ソリューションズ シード ユーエス エルエルシー 最適化塩基エディター
AU2023380182A1 (en) 2022-11-18 2025-07-03 Kyoto Prefectural Public University Corporation Compositions for mitophagy induction and uses thereof
WO2024133272A1 (en) 2022-12-21 2024-06-27 BASF Agricultural Solutions Seed US LLC Increased editing efficiency by co-delivery of rnp with nucleic acid
US20250001010A1 (en) 2023-06-30 2025-01-02 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
US20250319206A1 (en) 2024-04-04 2025-10-16 Christina Care Gene Editing Institute, Inc. Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2
WO2025226816A1 (en) 2024-04-23 2025-10-30 Christiana Care Gene Editing Institute, Inc. Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244591A (zh) * 2016-08-23 2016-12-21 苏州吉玛基因股份有限公司 修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用
WO2017064546A1 (en) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
WO2018027078A1 (en) * 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US12110490B2 (en) * 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
KR20260004568A (ko) 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
CN109790527A (zh) 2016-07-26 2019-05-21 通用医疗公司 普氏菌属和弗朗西斯氏菌属的CRISPR1(Cpf1)的变体
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
SG10201913505WA (en) 2016-10-17 2020-02-27 Univ Nanyang Tech Truncated crispr-cas proteins for dna targeting
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018195540A1 (en) 2017-04-21 2018-10-25 The General Hospital Corporation Inducible, tunable, and multiplex human gene regulation using crispr-cpf1
US11591589B2 (en) 2017-04-21 2023-02-28 The General Hospital Corporation Variants of Cpf1 (Cas12a) with altered PAM specificity
WO2018209014A1 (en) 2017-05-10 2018-11-15 Regents Of The University Of Minnesota Programmable transcription factors and methods
WO2018213726A1 (en) 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US11866697B2 (en) 2017-05-18 2024-01-09 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
KR102655021B1 (ko) 2017-07-11 2024-04-04 시그마-알드리치 컴퍼니., 엘엘씨 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US11649442B2 (en) 2017-09-08 2023-05-16 The Regents Of The University Of California RNA-guided endonuclease fusion polypeptides and methods of use thereof
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
US20210079366A1 (en) 2017-12-22 2021-03-18 The Broad Institute, Inc. Cas12a systems, methods, and compositions for targeted rna base editing
US11884947B2 (en) 2018-02-23 2024-01-30 Shanghaitech University Fusion proteins for base editing
KR102287880B1 (ko) 2018-03-26 2021-08-09 고쿠리츠다이가쿠호진 고베다이가쿠 세포에서 이중 가닥 dna의 표적 부위를 변형시키기 위한 방법
KR102922694B1 (ko) 2018-04-19 2026-02-03 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 유전자 편집을 위한 조성물 및 방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017064546A1 (en) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
WO2018027078A1 (en) * 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
CN106244591A (zh) * 2016-08-23 2016-12-21 苏州吉玛基因股份有限公司 修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EARL,A.等: "GenBank: EOS46485.1", 《GENBANK》 *
STEFANO STELLA等: "Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage", 《NATURE》 *
无: "NCBI Reference Sequence: WP_021736722.1", 《GENBANK》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119506249A (zh) * 2024-11-29 2025-02-25 大连理工大学 一种多PAM选择的高效基因编辑系统NovaCas-1及其应用
CN119506249B (zh) * 2024-11-29 2025-11-07 大连理工大学 一种多PAM选择的基因编辑系统NovaCas-1及其应用

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CA3102676A1 (en) 2019-12-12
JP2021525541A (ja) 2021-09-27
US12152257B2 (en) 2024-11-26
EP3802806A1 (en) 2021-04-14
BR112020024736A2 (pt) 2021-03-23
JP2025075025A (ja) 2025-05-14
US20210230567A1 (en) 2021-07-29
IL279153A (en) 2021-01-31
MA52739A (fr) 2021-04-21
US20250163394A1 (en) 2025-05-22
WO2019233990A1 (en) 2019-12-12
KR20210028163A (ko) 2021-03-11
MX2020013229A (es) 2021-04-28
AU2019282094A1 (en) 2021-01-07

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