BR112020024736A2 - endonucleases cpf1 mutantes - Google Patents
endonucleases cpf1 mutantes Download PDFInfo
- Publication number
- BR112020024736A2 BR112020024736A2 BR112020024736-7A BR112020024736A BR112020024736A2 BR 112020024736 A2 BR112020024736 A2 BR 112020024736A2 BR 112020024736 A BR112020024736 A BR 112020024736A BR 112020024736 A2 BR112020024736 A2 BR 112020024736A2
- Authority
- BR
- Brazil
- Prior art keywords
- cpf1
- seq
- sequence
- mutant
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18175707 | 2018-06-04 | ||
| EP18175707.1 | 2018-06-04 | ||
| EP18190950.8 | 2018-08-27 | ||
| EP18190950 | 2018-08-27 | ||
| PCT/EP2019/064444 WO2019233990A1 (en) | 2018-06-04 | 2019-06-04 | Mutant cpf1 endonucleases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112020024736A2 true BR112020024736A2 (pt) | 2021-03-23 |
Family
ID=66676556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112020024736-7A BR112020024736A2 (pt) | 2018-06-04 | 2019-06-04 | endonucleases cpf1 mutantes |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US12152257B2 (https=) |
| EP (1) | EP3802806A1 (https=) |
| JP (2) | JP2021525541A (https=) |
| KR (1) | KR20210028163A (https=) |
| CN (1) | CN112424349A (https=) |
| AU (1) | AU2019282094A1 (https=) |
| BR (1) | BR112020024736A2 (https=) |
| CA (1) | CA3102676A1 (https=) |
| IL (1) | IL279153A (https=) |
| MA (1) | MA52739A (https=) |
| MX (1) | MX2020013229A (https=) |
| WO (1) | WO2019233990A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| CN111521781B (zh) * | 2020-02-24 | 2021-07-30 | 广州医科大学附属第一医院（广州呼吸中心） | 一种用于新冠肺炎病毒SARS-CoV-2核酸的检测试剂盒及其检测方法 |
| JP7850156B2 (ja) * | 2020-12-17 | 2026-04-22 | モンサント テクノロジー エルエルシー | 操作されたｓｓＤＮａｓｅ不含ＣＲＩＳＰＲエンドヌクレアーゼ |
| EP4284923A1 (en) | 2021-01-29 | 2023-12-06 | Wageningen Universiteit | Crispr-associated base-editing of the complementary strand |
| US20250025572A1 (en) * | 2021-11-29 | 2025-01-23 | Editas Medicine, Inc. | Engineered crispr/cas12a effector proteins, and uses thereof |
| WO2023166030A1 (en) | 2022-03-01 | 2023-09-07 | BASF Agricultural Solutions Seed US LLC | Cas12a nickases |
| JP2025511267A (ja) | 2022-03-30 | 2025-04-15 | ビーエーエスエフ アグリカルチュラル ソリューションズ シード ユーエス エルエルシー | 最適化塩基エディター |
| AU2023380182A1 (en) | 2022-11-18 | 2025-07-03 | Kyoto Prefectural Public University Corporation | Compositions for mitophagy induction and uses thereof |
| WO2024133272A1 (en) | 2022-12-21 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Increased editing efficiency by co-delivery of rnp with nucleic acid |
| US20250001010A1 (en) | 2023-06-30 | 2025-01-02 | Christiana Care Gene Editing Institute, Inc. | Nras gene knockout for treatment of cancer |
| US20250319206A1 (en) | 2024-04-04 | 2025-10-16 | Christina Care Gene Editing Institute, Inc. | Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2 |
| WO2025226816A1 (en) | 2024-04-23 | 2025-10-30 | Christiana Care Gene Editing Institute, Inc. | Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes |
| CN119506249B (zh) * | 2024-11-29 | 2025-11-07 | 大连理工大学 | 一种多PAM选择的基因编辑系统NovaCas-1及其应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| US10648020B2 (en) | 2015-06-18 | 2020-05-12 | The Broad Institute, Inc. | CRISPR enzymes and systems |
| US20190048340A1 (en) * | 2015-09-24 | 2019-02-14 | Crispr Therapeutics Ag | Novel family of rna-programmable endonucleases and their uses in genome editing and other applications |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| US12110490B2 (en) * | 2015-12-18 | 2024-10-08 | The Broad Institute, Inc. | CRISPR enzymes and systems |
| EP3445853A1 (en) | 2016-04-19 | 2019-02-27 | The Broad Institute, Inc. | Cpf1 complexes with reduced indel activity |
| KR20260004568A (ko) | 2016-04-19 | 2026-01-08 | 더 브로드 인스티튜트, 인코퍼레이티드 | 신규한 crispr 효소 및 시스템 |
| CN106244591A (zh) * | 2016-08-23 | 2016-12-21 | 苏州吉玛基因股份有限公司 | 修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用 |
| CN109790527A (zh) | 2016-07-26 | 2019-05-21 | 通用医疗公司 | 普氏菌属和弗朗西斯氏菌属的CRISPR1(Cpf1)的变体 |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| SG10201913505WA (en) | 2016-10-17 | 2020-02-27 | Univ Nanyang Tech | Truncated crispr-cas proteins for dna targeting |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| WO2018195540A1 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Inducible, tunable, and multiplex human gene regulation using crispr-cpf1 |
| US11591589B2 (en) | 2017-04-21 | 2023-02-28 | The General Hospital Corporation | Variants of Cpf1 (Cas12a) with altered PAM specificity |
| WO2018209014A1 (en) | 2017-05-10 | 2018-11-15 | Regents Of The University Of Minnesota | Programmable transcription factors and methods |
| WO2018213726A1 (en) | 2017-05-18 | 2018-11-22 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
| US11866697B2 (en) | 2017-05-18 | 2024-01-09 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
| KR102655021B1 (ko) | 2017-07-11 | 2024-04-04 | 시그마-알드리치 컴퍼니., 엘엘씨 | 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US11649442B2 (en) | 2017-09-08 | 2023-05-16 | The Regents Of The University Of California | RNA-guided endonuclease fusion polypeptides and methods of use thereof |
| WO2019075197A1 (en) | 2017-10-11 | 2019-04-18 | The General Hospital Corporation | METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES |
| US20210079366A1 (en) | 2017-12-22 | 2021-03-18 | The Broad Institute, Inc. | Cas12a systems, methods, and compositions for targeted rna base editing |
| US11884947B2 (en) | 2018-02-23 | 2024-01-30 | Shanghaitech University | Fusion proteins for base editing |
| KR102287880B1 (ko) | 2018-03-26 | 2021-08-09 | 고쿠리츠다이가쿠호진 고베다이가쿠 | 세포에서 이중 가닥 dna의 표적 부위를 변형시키기 위한 방법 |
| KR102922694B1 (ko) | 2018-04-19 | 2026-02-03 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | 유전자 편집을 위한 조성물 및 방법 |
-
2019
- 2019-06-04 JP JP2020567958A patent/JP2021525541A/ja active Pending
- 2019-06-04 AU AU2019282094A patent/AU2019282094A1/en not_active Abandoned
- 2019-06-04 MA MA052739A patent/MA52739A/fr unknown
- 2019-06-04 CA CA3102676A patent/CA3102676A1/en active Pending
- 2019-06-04 EP EP19727424.4A patent/EP3802806A1/en not_active Withdrawn
- 2019-06-04 CN CN201980047153.2A patent/CN112424349A/zh active Pending
- 2019-06-04 WO PCT/EP2019/064444 patent/WO2019233990A1/en not_active Ceased
- 2019-06-04 KR KR1020207037564A patent/KR20210028163A/ko not_active Ceased
- 2019-06-04 US US15/734,839 patent/US12152257B2/en active Active
- 2019-06-04 MX MX2020013229A patent/MX2020013229A/es unknown
- 2019-06-04 BR BR112020024736-7A patent/BR112020024736A2/pt unknown
-
2020
- 2020-12-02 IL IL279153A patent/IL279153A/en unknown
-
2024
- 2024-10-24 US US18/925,815 patent/US20250163394A1/en active Pending
- 2024-12-23 JP JP2024226441A patent/JP2025075025A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA3102676A1 (en) | 2019-12-12 |
| JP2021525541A (ja) | 2021-09-27 |
| US12152257B2 (en) | 2024-11-26 |
| CN112424349A (zh) | 2021-02-26 |
| EP3802806A1 (en) | 2021-04-14 |
| JP2025075025A (ja) | 2025-05-14 |
| US20210230567A1 (en) | 2021-07-29 |
| IL279153A (en) | 2021-01-31 |
| MA52739A (fr) | 2021-04-21 |
| US20250163394A1 (en) | 2025-05-22 |
| WO2019233990A1 (en) | 2019-12-12 |
| KR20210028163A (ko) | 2021-03-11 |
| MX2020013229A (es) | 2021-04-28 |
| AU2019282094A1 (en) | 2021-01-07 |
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| Date | Code | Title | Description |
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| B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
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