CN112400896A - MiYou xi pomelo scab prevention and control bactericide and preparation method thereof - Google Patents

MiYou xi pomelo scab prevention and control bactericide and preparation method thereof Download PDF

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Publication number
CN112400896A
CN112400896A CN202011382900.9A CN202011382900A CN112400896A CN 112400896 A CN112400896 A CN 112400896A CN 202011382900 A CN202011382900 A CN 202011382900A CN 112400896 A CN112400896 A CN 112400896A
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bactericide
water emulsion
antibacterial
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controlling
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蓝炎阳
林荣耀
段敬庭
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Shengzhou Nuosheng Agricultural Development Co ltd
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Shengzhou Nuosheng Agricultural Development Co ltd
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Abstract

The invention discloses a bactericide for preventing and controlling a black star of a Mixi GUAN, which is a water emulsion-shaped bactericide, and the water emulsion-shaped bactericide mainly comprises the following components in 1000g by weight: 100-180g of antibacterial pesticide, 50-80g of allicin, 20-40g of chlorophyll, 28-45g of chitosan, 10-16g of polydopamine, 3-8g of plant activator benzothiadiazole, 2-6g of pectinase crude extract, 2-6g of SOD enzyme, 6-12g of potassium carbonate, 8-11g of surfactant, 20-40g of film-spreading oil agent and 20-35g of emulsifier, and the balance of water. According to the invention, the alkaline pesticide is matched with potassium carbonate to adjust the pH value, and the sterilization and nutrition factors are added, so that the resistance of the honey pomelo tree is enhanced, the fruit quality is improved, the synthesis of lignin in the honey pomelo peel is induced, the systemic resistance of the honey pomelo is improved, the surface of the honey pomelo is kept dry by utilizing the characteristics of the film spreading oil agent, and a part of antibacterial factors are locked, so that the honey pomelo has a long-term antibacterial effect.

Description

MiYou xi pomelo scab prevention and control bactericide and preparation method thereof
Technical Field
The invention relates to the field of pesticides, in particular to a bactericide for preventing and controlling a black star of MiYou xi GUAN and a preparation method thereof.
Background
Scab is caused by an infection with the fungus Deuteromycetes, Cladosporium cucumerinum. The germs live through the winter in the fields, in the soil and on the trellis materials of the greenhouse by using mycelium or conidium as the disease residues, and become the initial infection source in the next year. Seeds can also carry bacteria to cause disease. Pathogenic bacteria mainly penetrate through the epidermis directly or invade from pores and wounds to cause diseases, and conidia generated on diseased plants are spread in the field by airflow, rainwater sputtering and farming operation. The growth and development temperature of pathogenic bacteria is 2-35 ℃, the suitable temperature is 20-22 ℃, conidia are easily generated at the relative humidity of more than 93% and the daily average temperature of 15-30 ℃, the conidia can germinate at the temperature of 5-30 ℃ when the relative humidity of 100% is the most, and the suitable temperature is 15-25 ℃ and requires water drops and nutrition. When the minimum field temperature is above 10 ℃, the relative humidity is higher than 90% for a long time, and the condensation of the leaf surfaces of plants occurs seriously. The rain water is much, the rain quantity is large, and the temperature is low in the growing period of the host crops, so that the disease is easy to occur. The scab causes great loss to fruit growers every year.
Since scab may invade in young fruit stage and manifests itself only when the fruit is near mature stage, it is difficult to prevent and treat in advance, the existing bactericide can only reduce the damage of scab to a certain extent, but its effect is limited, and still has great disease loss.
Therefore, it is necessary to invent a bactericide for preventing and controlling the melasma of Mixi GUAN and its preparation method to solve the above problems.
Disclosure of Invention
The invention aims to provide a bactericide for preventing and controlling the Venturia xi Mi shaddock from the early area and a preparation method thereof, which can inactivate Cladosporium cucumerinum fungi without damaging probiotics and tree tissues by using an alkaline pesticide and potassium carbonate to regulate the pH value, and can enhance the resistance of the tree body of the Mi shaddock by adding chlorophyll, chitosan, polydopamine, a plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, improve the fruit quality, induce the synthesis of lignin in the pericarp of the Mi shaddock, improve the system resistance of the Mi shaddock, keep the surface of the Mi shaddock dry and lock a part of antibacterial factors by utilizing the characteristics of a film-spreading oil agent, so that the Mi shaddock has a long-term antibacterial effect and overcomes the defects in the technology.
In order to achieve the above purpose, the invention provides the following technical scheme: the bactericide for preventing and controlling the myxoma xi pum and the scab of the myxoma xi pumper 1000g mainly comprises the following components in parts by weight:
100-180g of antibacterial pesticide, 50-80g of allicin, 20-40g of chlorophyll, 28-45g of chitosan, 10-16g of polydopamine, 3-8g of plant activator benzothiadiazole, 2-6g of pectinase crude extract, 2-6g of SOD enzyme, 6-12g of potassium carbonate, 8-11g of surfactant, 20-40g of film-spreading oil agent and 20-35g of emulsifier, and the balance of water.
Preferably, the antibacterial pesticide is one of lime sulphur, bordeaux mixture, nitrile mancozeb or zineb.
Preferably, the surfactant is one of quaternary ammonium glucoside and algal glycolipid microbial surfactants.
Preferably, the emulsifier is cis-9-octadecenoic acid, and the membrane spreading oil agent is 30% poison/thiadiazole membrane spreading oil agent.
A preparation method of a bactericide for preventing and controlling a myYou xi pomelo scab comprises the following specific operation steps:
the method comprises the following steps: preparing raw materials, namely preparing antibacterial pesticide, allicin, chlorophyll, chitosan, polydopamine, plant activator benzothiadiazole, pectinase crude extract, SOD enzyme, potassium carbonate, surfactant, film spreading oil agent and emulsifier according to a formula ratio;
step two: mixing chitosan and polydopamine as carrier, adding into liquid culture medium, moistening, injecting plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, standing at room temperature for 12 hr, and preparing defense supply matrix;
step three: adding allicin and chlorophyll into antibacterial pesticide, adding potassium carbonate, stirring, measuring pH of the antibacterial pesticide mixture to make it alkaline, and stirring to obtain antibacterial liquid medicine;
step four: adding the defense supply matrix prepared in the step two into the antibacterial liquid medicine, adding a surfactant, uniformly stirring, filtering impurities by using a centrifugal machine, and taking supernatant for later use;
step five: adding the spreading oil agent and the emulsifier into the supernatant, stirring uniformly, emulsifying to obtain water emulsion bactericide, filling, and storing in a cool and dry place.
Preferably, the addition of potassium carbonate is stopped when the pH value measured in step three is 7.5-8.2.
Preferably, the emulsification reaction temperature in the fifth step is set to be 20-30 ℃, and the emulsification environment is vacuum.
A method for using a bactericide for preventing and controlling the myxoma xi pum, the water emulsion bactericide is applied by an atomization spraying method and is mixed with water for spraying, and the mass mixing ratio of the water emulsion bactericide to the water is set as 1: 500-600.
Preferably, the application period of the water emulsion-shaped bactericide is 12-14 days, if the water emulsion-shaped bactericide meets rainy days, a proper amount of the bactericide is supplemented, the bactericide is applied under clear weather at two or three points in the afternoon, and the antibacterial pesticide in the bactericide is replaced and used alternately when the water emulsion-shaped bactericide is continuously used.
In the technical scheme, the invention provides the following technical effects and advantages:
1. the alkaline pesticide is used for matching with potassium carbonate to adjust the pH value, so that the fungi of the fungi imperfecti cucurbita scabra is inactivated without damaging probiotics and tree tissues, the environment is protected, trace elements of honey pomelos are further increased, nutrition improvement is carried out, the green phyllanthin is added to be used as a water soluble fertilizer to be sprayed on the surfaces of the tree tissues, absorption of the fruit trees is promoted, the autoimmunity of the fruit trees is enhanced, chitosan and polydopamine have antibacterial effects, meanwhile, pectinase and SOD enzyme can be adsorbed and cultured as carriers, and the plant activator benzothiadiazole is matched to have a certain control effect on germs of the fruit trees, so that the resistance of the trees of the honey pomelos can be enhanced, the fruit quality is improved, the fruit surfaces are colored and can be stored for a long time, synthesis of lignin in the peel of the honey pomelos can be induced, the systemic resistance of the honey pomelos is improved, the damage of the fungi imperfecti cucumis melo scabra fungus to fruits is, the film-spreading oil agent can be fully mixed in the bactericide, and is sprayed by adding water when in use, the film-spreading oil agent is remained on the surface of the pomelo fruit and locks a part of antibacterial factors, so that the pomelo fruit has a long-term antibacterial effect, and the germination probability of the fungi imperfecti cucurbita scabra fungus can be greatly reduced when the pomelo fruit is kept in a dry state;
2. by adopting one of lime sulfur, Bordeaux mixture, nitrile manganese zinc and zineb as the antibacterial pesticide, the water is rapidly evaporated by two-point to three-point spraying in the afternoon in clear weather, so that the loss of the pesticide is reduced, the disease resistance of the Mixi Yan Guo is obviously improved by alternately using the antibacterial pesticide in the sterilizer when the water emulsion bactericide is continuously used, the recovery ratio of good fruits of the Mixi Yan Guo is improved, the sterilization and the antibiosis are effectively realized, and the drug resistance and the damage to fruit trees are reduced.
Detailed Description
The present invention will be described in further detail below in order to enable those skilled in the art to better understand the technical solution of the present invention.
Example 1:
the invention provides a bactericide for preventing and controlling a black star of a Mixi GUAN, which is a water emulsion bactericide, and the water emulsion bactericide mainly comprises the following components in 1000g by weight:
100g of antibacterial pesticide, 50g of allicin, 20g of chlorophyll, 28g of chitosan, 10g of polydopamine, 3g of diazosulfide serving as a plant activator, 2g of pectinase crude extract, 2g of SOD enzyme, 6g of potassium carbonate, 8g of surfactant, 20g of spreading oil agent and 20g of emulsifier, and the balance of water.
Further, in the above technical solution, the antibacterial pesticide is one of lime sulphur, bordeaux mixture, nitrile bacteria mancozeb or zineb.
Further, in the above technical solution, the surfactant is one of a quaternary ammonium glucoside and a seaweed glycolipid microbial surfactant.
Further, in the above technical scheme, the emulsifier is cis-9-octadecenoic acid, and the membrane-spreading oil is 30% poison/thiadiazole membrane oil.
A preparation method of a bactericide for preventing and controlling a myYou xi pomelo scab comprises the following specific operation steps:
the method comprises the following steps: preparing raw materials, namely preparing antibacterial pesticide, allicin, chlorophyll, chitosan, polydopamine, plant activator benzothiadiazole, pectinase crude extract, SOD enzyme, potassium carbonate, surfactant, film spreading oil agent and emulsifier according to a formula ratio;
step two: mixing chitosan and polydopamine as carrier, adding into liquid culture medium, moistening, injecting plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, standing at room temperature for 12 hr, and preparing defense supply matrix;
step three: adding allicin and chlorophyll into antibacterial pesticide, adding potassium carbonate, stirring, measuring pH of the antibacterial pesticide mixture to make it alkaline, stopping adding potassium carbonate when pH is 7.5, and stirring to obtain antibacterial liquid medicine;
step four: adding the defense supply matrix prepared in the step two into the antibacterial liquid medicine, adding a surfactant, uniformly stirring, filtering impurities by using a centrifugal machine, and taking supernatant for later use;
step five: adding the spreading oil agent and the emulsifier into the supernatant, stirring uniformly, setting the emulsification reaction temperature at 20 ℃, and the emulsification environment at vacuum, preparing the water emulsion bactericide after emulsification, filling the water emulsion bactericide, and storing the water emulsion bactericide in a cool and dry place.
A method for using a bactericide for preventing and controlling the myxoma xi pum, the water emulsion bactericide is applied by an atomization spraying method and is mixed with water for spraying, and the mass mixing ratio of the water emulsion bactericide to the water is set as 1: 500.
further, in the technical scheme, the application period of the water emulsion bactericide is 12 days, if the water emulsion bactericide meets rainy days, a proper amount of the bactericide is supplemented, the water emulsion bactericide is sprayed at two or three points in the afternoon under clear weather, and the antibacterial pesticide in the sterilizer is replaced and alternately used when the water emulsion bactericide is continuously used.
The bactericide prepared in the embodiment is of a water emulsion-shaped structure with uniform texture, has a good mixing effect with water, is dissolved quickly, and has high drug effect content, the bactericide prepared in the embodiment is used for preventing and spraying the myxoma xi pomelo scab, the prevention effect of the bactericide after 1d after spraying is 61.52%, the prevention effect of the bactericide after 7d after spraying is 84.35%, the prevention effect of the bactericide after 14d after spraying is 79.39% and is obviously superior to that of the existing similar products after spraying, the annual yield of the good products of the myxoma xi pomelo is improved by 35.4% in a year by using the bactericide and the conventional bactericide products, and the obvious improvement is achieved.
Example 2:
the invention provides a bactericide for preventing and controlling a black star of a Mixi GUAN, which is a water emulsion bactericide, and the water emulsion bactericide mainly comprises the following components in 1000g by weight:
150g of antibacterial pesticide, 60g of allicin, 30g of chlorophyll, 40g of chitosan, 15g of polydopamine, 5g of plant activator benzothiadiazole, 4g of pectinase crude extract, 4g of SOD enzyme, 10g of potassium carbonate, 9g of surfactant, 30g of film-spreading oil agent and 25g of emulsifier, and the balance of water.
Further, in the above technical solution, the antibacterial pesticide is one of lime sulphur, bordeaux mixture, nitrile bacteria mancozeb or zineb.
Further, in the above technical solution, the surfactant is one of a quaternary ammonium glucoside and a seaweed glycolipid microbial surfactant.
Further, in the above technical scheme, the emulsifier is cis-9-octadecenoic acid, and the membrane-spreading oil is 30% poison/thiadiazole membrane oil.
A preparation method of a bactericide for preventing and controlling a myYou xi pomelo scab comprises the following specific operation steps:
the method comprises the following steps: preparing raw materials, namely preparing antibacterial pesticide, allicin, chlorophyll, chitosan, polydopamine, plant activator benzothiadiazole, pectinase crude extract, SOD enzyme, potassium carbonate, surfactant, film spreading oil agent and emulsifier according to a formula ratio;
step two: mixing chitosan and polydopamine as carrier, adding into liquid culture medium, moistening, injecting plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, standing at room temperature for 12 hr, and preparing defense supply matrix;
step three: adding allicin and chlorophyll into antibacterial pesticide, adding potassium carbonate, stirring, measuring pH of the antibacterial pesticide mixture to make it alkaline, stopping adding potassium carbonate when pH is 8.0, and stirring to obtain antibacterial liquid medicine;
step four: adding the defense supply matrix prepared in the step two into the antibacterial liquid medicine, adding a surfactant, uniformly stirring, filtering impurities by using a centrifugal machine, and taking supernatant for later use;
step five: adding the spreading oil agent and the emulsifier into the supernatant, stirring uniformly, setting the emulsification reaction temperature at 25 ℃ and the emulsification environment at vacuum, preparing the water emulsion bactericide after emulsification, filling the water emulsion bactericide, and storing the water emulsion bactericide in a cool and dry place.
A method for using a bactericide for preventing and controlling the myxoma xi pum, the water emulsion bactericide is applied by an atomization spraying method and is mixed with water for spraying, and the mass mixing ratio of the water emulsion bactericide to the water is set as 1: 550.
further, in the above technical scheme, the application period of the water emulsion-shaped bactericide is 13 days, if meeting rain weather, a proper amount of the bactericide is supplemented, the application environment is that the bactericide is sprayed at two or three points in the afternoon under clear weather, and the antibacterial pesticide in the sterilizer is replaced and used alternately when the water emulsion-shaped bactericide is continuously used.
Compared with the example 1, the bactericide prepared in the example is a water emulsion-like structure with uniform texture, is good in mixing effect with water, is fast to dissolve and has high drug effect content, the bactericide prepared in the example is used for preventing and spraying the myxoma xi Mi Guo from the scab, the prevention effect of the bactericide after 1d of spraying is 61.47 percent, the prevention effect of the bactericide after 7d of spraying is 87.81 percent and the prevention effect of the bactericide after 14d of spraying is 81.23 percent after the detection and further calculation according to the good fruit rate and the sick fruit rate, the prevention effect is obviously better than that of the existing similar products, the annual yield of the good product of the myxoma Mi Guo is improved by 49.6 percent compared with the conventional bactericide product, and the obvious progress is achieved.
Example 3:
the invention provides a bactericide for preventing and controlling a black star of a Mixi GUAN, which is a water emulsion bactericide, and the water emulsion bactericide mainly comprises the following components in 1000g by weight:
180g of antibacterial pesticide, 80g of allicin, 40g of chlorophyll, 45g of chitosan, 16g of polydopamine, 8g of diazosulfide serving as a plant activator, 6g of pectinase crude extract, 6g of SOD enzyme, 12g of potassium carbonate, 11g of surfactant, 40g of film spreading oil agent and 35g of emulsifier, and the balance of water.
Further, in the above technical solution, the antibacterial pesticide is one of lime sulphur, bordeaux mixture, nitrile bacteria mancozeb or zineb.
Further, in the above technical solution, the surfactant is one of a quaternary ammonium glucoside and a seaweed glycolipid microbial surfactant.
Further, in the above technical scheme, the emulsifier is cis-9-octadecenoic acid, and the membrane-spreading oil is 30% poison/thiadiazole membrane oil.
A preparation method of a bactericide for preventing and controlling a myYou xi pomelo scab comprises the following specific operation steps:
the method comprises the following steps: preparing raw materials, namely preparing antibacterial pesticide, allicin, chlorophyll, chitosan, polydopamine, plant activator benzothiadiazole, pectinase crude extract, SOD enzyme, potassium carbonate, surfactant, film spreading oil agent and emulsifier according to a formula ratio;
step two: mixing chitosan and polydopamine as carrier, adding into liquid culture medium, moistening, injecting plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, standing at room temperature for 12 hr, and preparing defense supply matrix;
step three: adding allicin and chlorophyll into antibacterial pesticide, adding potassium carbonate, stirring, measuring pH of the antibacterial pesticide mixture to make it alkaline, stopping adding potassium carbonate when pH is 8.2, and stirring to obtain antibacterial liquid medicine;
step four: adding the defense supply matrix prepared in the step two into the antibacterial liquid medicine, adding a surfactant, uniformly stirring, filtering impurities by using a centrifugal machine, and taking supernatant for later use;
step five: adding the spreading oil agent and the emulsifier into the supernatant, stirring uniformly, setting the emulsification reaction temperature at 30 ℃ and the emulsification environment at vacuum, preparing the water emulsion bactericide after emulsification, filling the water emulsion bactericide, and storing the water emulsion bactericide in a cool and dry place.
A method for using a bactericide for preventing and controlling the myxoma xi pum, the water emulsion bactericide is applied by an atomization spraying method and is mixed with water for spraying, and the mass mixing ratio of the water emulsion bactericide to the water is set as 1: 600.
further, in the technical scheme, the application period of the water emulsion bactericide is 14 days, if the water emulsion bactericide meets rainy days, a proper amount of the bactericide is supplemented, the water emulsion bactericide is sprayed in a clear environment at two or three points in the afternoon, and the antibacterial pesticide in the sterilizer is replaced and alternately used when the water emulsion bactericide is continuously used.
Comparing examples 1 and 2, the bactericide prepared in this example is a water emulsion structure with uniform texture, good mixing effect with water, fast dissolution and high drug effect, the bactericide prepared in this example is used for preventing and spraying the myxoma xi Mi Guo, the prevention effect of the bactericide after 1d after spraying is 61.76%, the prevention effect of the bactericide after 7d after spraying is 85.22%, the prevention effect of the bactericide after 14d after spraying is 82.01% after detecting the occurrence of the myxoma Mi Guo Black Star and further calculating according to the good fruit rate and the disease fruit rate, the prevention effect is obviously better than that of the existing similar products, the annual yield of the good myxoma Mi Guo is improved by 46.7% after using the bactericide and the conventional bactericide products, and the obvious progress is achieved.
The following table is obtained according to examples 1 to 3:
Figure BDA0002810053010000081
from the above table, it can be seen that in example 2, the raw material proportion is moderate, the processing temperature is moderate, and the processing technology is most suitable for preparing the bactericide for preventing and controlling the cladosporium cucumerinum of the xi honey pomelo, the bactericide prepared in this example has the best curative effect, the disease resistance of the xi honey pomelo is obviously improved, the recovery ratio of good fruits of the xi honey pomelo is improved, the bactericide is effective in sterilization and antibiosis, and has no drug resistance, the production cost is reduced, and the income of fruit growers is greatly improved at the same time, in the technical scheme, one of the mixture of lime sulfur, boldo liquid, nitrile bacteria manganese zinc and zineb is adopted as an antibacterial pesticide, the alkaline pesticide is matched with potassium carbonate to adjust the pH value, so that the Cladosporium cucumerinum fungus is inactivated without damaging probiotics and tree tissues, the environment is protected, trace elements of the honey pomelo are further increased, the nutrition is improved, and the chlorophyllin is added as a water soluble fertilizer to be sprayed on, promoting absorption of fruit trees, enhancing the immunity of the fruit trees, having antibacterial effect of chitosan and polydopamine, meanwhile, can be used as a carrier to adsorb and culture pectinase and SOD enzyme, is matched with a plant activator benzothiadiazole, the fruit tree preservative has certain control effect on fruit tree germs, can enhance the resistance of honey pomelo trees, improve the fruit quality, has good fruit surface coloring and storage resistance, can induce the synthesis of lignin in honey pomelo peels, improve the system resistance of the honey pomelo, reduce the damage of fungi of deuteromycete cucurbita scabbing branch sporotrichia to fruits, and the technical scheme also adds a surfactant and a film-spreading oil agent to be matched with an emulsifier for stirring, so that the film-spreading oil agent can be fully mixed in a bactericide and is added with water for spraying when in use, the film-spreading oil agent is retained on the surface of the pomelo fruit and locks a part of antibacterial factors, so that the pomelo fruit has a long-term antibacterial effect, and the germination probability of fungi imperfecti cucurbita scab mycoderma can be greatly reduced under the condition that the pomelo fruit is kept in a dry state.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the invention. Therefore, the above description is illustrative in nature and should not be construed as limiting the scope of the invention, which is defined by the appended claims, and any modifications, equivalents, improvements and the like, which come within the spirit and scope of the invention are intended to be embraced therein.

Claims (9)

1. A bactericide for preventing and controlling a myYou xi GUAN from a scab, which is characterized in that: the bactericide is a water emulsion bactericide, and the water emulsion bactericide mainly comprises the following components in parts by weight per 1000 g:
100-180g of antibacterial pesticide, 50-80g of allicin, 20-40g of chlorophyll, 28-45g of chitosan, 10-16g of polydopamine, 3-8g of plant activator benzothiadiazole, 2-6g of pectinase crude extract, 2-6g of SOD enzyme, 6-12g of potassium carbonate, 8-11g of surfactant, 20-40g of film-spreading oil agent and 20-35g of emulsifier, and the balance of water.
2. The bactericide for preventing and controlling the asterism of Mixi GUAN, as claimed in claim 1, wherein: the antibacterial pesticide is one of lime sulfur, Bordeaux mixture, nitrile bacteria manganese zinc or zineb.
3. The bactericide for preventing and controlling the asterism of Mixi GUAN, as claimed in claim 1, wherein: the surfactant is one of quaternary ammonium glucoside and seaweed glycolipid microbial surfactants.
4. The bactericide for preventing and controlling the asterism of Mixi GUAN, as claimed in claim 1, wherein: the emulsifier is cis-9-octadecenoic acid, and the membrane spreading oil agent is 30% poison and thiadiazole spreading oil agent.
5. A preparation method of a bactericide for preventing and controlling a myYou xi pomelo scab is characterized in that: the specific operation steps are as follows:
the method comprises the following steps: preparing raw materials, namely preparing antibacterial pesticide, allicin, chlorophyll, chitosan, polydopamine, plant activator benzothiadiazole, pectinase crude extract, SOD enzyme, potassium carbonate, surfactant, film spreading oil agent and emulsifier according to a formula ratio;
step two: mixing chitosan and polydopamine as carrier, adding into liquid culture medium, moistening, injecting plant activator benzothiadiazole, pectinase crude extract and SOD enzyme, standing at room temperature for 12 hr, and preparing defense supply matrix;
step three: adding allicin and chlorophyll into antibacterial pesticide, adding potassium carbonate, stirring, measuring pH of the antibacterial pesticide mixture to make it alkaline, and stirring to obtain antibacterial liquid medicine;
step four: adding the defense supply matrix prepared in the step two into the antibacterial liquid medicine, adding a surfactant, uniformly stirring, filtering impurities by using a centrifugal machine, and taking supernatant for later use;
step five: adding the spreading oil agent and the emulsifier into the supernatant, stirring uniformly, emulsifying to obtain water emulsion bactericide, filling, and storing in a cool and dry place.
6. The method of claim 5, wherein the bactericide for preventing and controlling the Vena Mixi Guo disease comprises: and (3) stopping adding the potassium carbonate when the pH value is 7.5-8.2 in the third step.
7. The method of claim 5, wherein the bactericide for preventing and controlling the Vena Mixi Guo disease comprises: and in the fifth step, the emulsification reaction temperature is set to be 20-30 ℃, and the emulsification environment is vacuum.
8. A method for using a bactericide for preventing and controlling a myYou xi GUAN from a scab disease, is characterized in that: the water emulsion bactericide is applied by an atomization spraying method and sprayed by adding water, and the mass mixing ratio of the water emulsion bactericide to the water is set as 1: 500-600.
9. The method of using a bactericidal agent for preventing and controlling a melasma of a Mixi GUAN, according to claim 8, wherein: the application period of the water emulsion-shaped bactericide is 12-14 days, if meeting rain weather, a proper amount of tonic is applied, the application environment is that the bactericide is sprayed at two or three points in the afternoon under clear weather, and the antibacterial pesticide in the bactericide is replaced and used alternately when the water emulsion-shaped bactericide is continuously used.
CN202011382900.9A 2020-12-01 2020-12-01 MiYou xi pomelo scab prevention and control bactericide and preparation method thereof Pending CN112400896A (en)

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