CN112400502A - Method for rapidly rooting sympodial bamboo branches and application thereof - Google Patents
Method for rapidly rooting sympodial bamboo branches and application thereof Download PDFInfo
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- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention belongs to the technical field of vegetative propagation of plants, and relates to a method for rapidly rooting sympodial bamboo branches and application thereof. The method comprises the steps of selecting 1-year-old thick and strong main branches or secondary branches of the sympodial bamboos which grow for more than 2 years and do not have diseases and insect pests, soaking branch roots in clear water in winter, keeping the temperature of water constant, filling air into the water and oxygenating the water, promoting the branch roots to take roots early and take multiple roots, transplanting the rooted branches, and improving the reproduction rate of the sympodial bamboos. The method is simple and easy to operate, the stump is soaked by clear water to ensure that the branches do not lose water, the branches and the stumps are promoted to take roots early and the branches have more roots through constant-temperature treatment of water, and tender roots are ensured not to rot through air filling and oxygenation of the water, so that the propagation rate of the branches is improved, the branch seedlings are cultivated in the same year and can be used for afforestation in the same year, the method is very suitable for application and popularization in production, and the economic benefit, the social benefit and the ecological benefit are good.
Description
Technical Field
The invention belongs to the technical field of vegetative propagation of plants, and particularly relates to a method for rapidly rooting sympodial bamboo branches and application thereof.
Background
The sympodial bamboos are not easy to seed after blooming and cannot be propagated sexually. Vegetative propagation is a common method, with shoot cutting being the most common method of propagation. Other methods of propagation are more difficult to apply. For example, a large amount of strong bamboo stalks are needed for stalk burying, joint burying, stump burying and the like, and the damage to bamboo forests caused by bamboo stalk collection, bamboo stump seedling raising or afforestation is large. Moreover, at present, no enough bamboo forest area is available for collecting bamboo stalks and bamboo stumps, so that the methods are not beneficial to large-scale seedling culture; the tissue culture method is still immature in technology, is limited by weak outdoor stress resistance of seedlings and the like, and cannot be popularized and applied in production, so that the method for cutting the main branches and the secondary branches is mostly adopted for raising the seedlings of the sympodial bamboos.
In the process of branch cuttage, the branches are in false survival, namely, most branches are subjected to branch drawing and leaf spreading at the early stage of the cuttage, and the twigs continuously wither and die after 15 days. The key influencing the survival rate of the cuttage of the branches is whether the branches can rapidly take root, and the roots of the bamboo branches are expected to survive before the nutrients carried by the bamboo branches are consumed, so that the roots are not gradually withered.
Disclosure of Invention
The invention aims to provide a rapid rooting method for sympodial bamboo branches, aiming at overcoming the defects of the traditional vegetative propagation technology of the sympodial bamboo.
The technical scheme of the invention is as follows:
the method comprises the steps of soaking 1-year-old thick and strong main branches and secondary branch roots of the sympodial bamboos in clear water, promoting the branch roots to grow early and more roots by means of keeping the water body at a constant temperature, filling air and oxygenating, transplanting the rooted branches, and improving the reproduction rate of the sympodial bamboos.
The invention is realized by the following steps:
a method for rapidly rooting sympodial bamboo branches comprises the procedures of branch selection, branch collection, water temperature control, air filling and oxygen charging, branch management and the like, and specifically comprises the following steps:
(1) selecting branches: selecting 1-year-old and robust main branches and secondary branches of sympodial bamboos;
(2) branch collection: collecting main branches and secondary branches in winter, and peeling sheaths, cutting stalks and removing leaves;
(3) controlling water temperature: pretreating the branch stump part stripped with the branch sheaths, and then placing the branch stump part in clean water at the constant temperature of 20-26 ℃, wherein the distance between the branch stump part and the water surface is 10-12 cm;
(4) filling air and oxygenating: inserting an inflation pipe into the cultivation barrel for soaking the branch and stump, and filling air into the clear water soaking solution after electrifying;
(5) and (3) cutting management: cutting indoors to avoid direct sunlight, maintaining the temperature of clear water soaking solution at 20-26 deg.C and water depth of 10-12cm, and maintaining aeration, and changing water 1 time every 3-4 days.
Preferably, the selection time of the branches is 1-2 months per year.
Preferably, more than 2-year-old sympodial bamboos without diseases and insect pests are selected, and then 1-year-old main branches or secondary branches are selected from the sympodial bamboos, the branches of the main branches or the secondary branches are bright green, slightly powdery, full in branch stumps, and the bamboo sheaths of the stump parts are not rotted.
Preferably, the branches and roots of the branches collected in the step (2) are not cracked, and the bud of the branch and root is not damaged.
Preferably, the placement mode of the branches in the step (3) is vertical placement or inclined placement.
Preferably, the pretreatment in the step (3) is to soak the stump part of the branch with a rooting powder aqueous solution with the concentration of 50-100 mg/L.
Preferably, the temperature of the constant temperature clear water in the step (3) is 22 ℃.
Preferably, the method for managing cuttings in step (5) comprises: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 20-26 ℃ at a constant temperature, air is continuously filled, water is changed for 1 time every 3-4 days, and the rooting condition of branches is checked every day.
Compared with the prior art, the invention has the following advantages:
(1) the branches are not dehydrated and withered within 1 month after being separated from the mother bamboos, so that conditions are created for promoting rooting;
(2) the branches are short, the transportation is simple, the wound treatment is not needed, and the working procedures are reduced;
(3) the invention changes the normal temperature treatment into the constant temperature treatment, the rooting time is advanced, the roots are more and orderly, the roots are promoted in winter in the current year, the roots can be transplanted to a container in spring or directly afforested, and the innovation effect is obvious;
(4) the method advances the seedling raising time of the sympodial bamboos to winter, utilizes low temperature to inhibit the sprouting of the branches and buds, reduces the nutrition consumption during the rooting period, and reduces the false survival phenomenon.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further clearly understood from the specific examples given below, which are not intended to limit the present invention.
The implementation place of the embodiment and the comparative example is south China Guangxi city, the implementation time is 1-2 months, the average high temperature of the air temperature is 15 ℃ and the average low temperature is 10 ℃;
the rooting powder used in this example was 5% Guoguanpan, produced by Sichuan Guoguang agro-chemical Co.
Example 1
A method for rapidly rooting sympodial bamboo branches specifically comprises the following steps:
(1) selecting branches: selecting a 2-year-old main branch without diseases and insect pests of the sympodial bamboos in No. 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 1.8 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 nodes of the branch with stump are left, the length of the branch is 50cm, no leaves are left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: soaking the stump part of the branch with the branch sheaths in 50mg/L rooting powder aqueous solution for 2h, then placing the branch in clean water at the constant temperature of 20 ℃, vertically placing the branch, keeping the stump part of the branch at the constant temperature of the water by using an automatic water temperature controller, and keeping the water at the constant temperature;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 20 ℃, air is kept to be filled continuously, and water is changed for 1 time every 3 days.
Example 2
(1) Selecting branches: selecting a secondary branch of 3-year-old sympodial bamboos without diseases and insect pests in No. 1 month 15, wherein the secondary branch is 1 year old and the diameter of a first section of the base of the secondary branch is 2.5 cm;
(2) branch collection: cutting off the joint of the main branch stump part and the secondary branch stump part to obtain secondary branches, cutting off tip parts of the secondary branches to leave 3 nodes of branches with stumps, the branches are 55cm long, leaves are not left, branch sheaths of the branch stump parts are peeled off after collection, and shading and moisturizing are carried out;
(3) controlling water temperature: soaking the stump part of the branch with the branch sheaths in 100mg/L rooting powder aqueous solution for 3h, pretreating with the rooting powder aqueous solution, placing in clean water at the constant temperature of 26 ℃, vertically placing the branch, keeping the stump part of the branch 12cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 26 ℃, air is kept to be filled continuously, and water is changed for 1 time every 3 days.
Example 3
(1) Selecting branches: selecting a 2-year-old main branch without plant diseases and insect pests of the sympodial bamboos in No. 30 of 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 2.2 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the tip of the main branch is cut off, 2.5 knots of a branch with the stump are left, the length of the branch is 52cm, no leaves are left, the branch stump part and the branch stump sheaths are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: soaking the stump part of the branch with the branch sheaths in 60mg/L rooting powder aqueous solution for 2.5h, then placing the branch in clean water at the constant temperature of 22 ℃, obliquely placing the branch with the stump part 11cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 22 ℃, air is kept to be filled continuously, and water is changed for 1 time every 4 days.
Example 4
(1) Selecting branches: selecting a secondary branch of 2-year-old sympodial bamboos without diseases and insect pests in No. 1 month 12, wherein the secondary branch is 1 year old and the diameter of a first section of the base of the secondary branch is 3.0 cm;
(2) branch collection: cutting off the joint of the main branch stump part and the secondary branch stump part to obtain secondary branches, cutting off tip parts of the secondary branches to leave 2 nodes of branches with stumps, the branches are 52cm long, leaves are not left, the branch stump part and branch sheaths are peeled off after collection, and shading and moisturizing are carried out;
(3) controlling water temperature: soaking the stump part of the branch with the branch sheaths in 80mg/L rooting powder aqueous solution for 3h, then placing the branch in clean water at the constant temperature of 24 ℃, obliquely placing the branch, keeping the stump part of the branch 12cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 24 ℃, air is kept to be filled continuously, water is changed for 1 time every 4 days, and rooting conditions of branches are checked every day.
Comparative example 1
(1) Selecting branches: selecting a boughs of a sympodial bamboo which grows for 2 years and has no plant diseases and insect pests at No. 1 month, wherein the boughs grow for 1 year and the diameter of a first section of the base of the boughs is 1.7 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 nodes of the branch with the stump are left, the length of the branch is 50cm, no leaves are left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: pre-soaking the stump part of the branch with the branch sheaths in 50mg/L rooting powder aqueous solution for 2h, then placing the branch in clean water at the constant temperature of 20 ℃, vertically placing the branch, keeping the stump part of the branch at the constant temperature of the water by 12cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 20 ℃, air is kept to be filled continuously, and water is changed for 1 time every 3 days.
Comparative example 2
(1) Selecting branches: selecting a 2-year-old main branch without diseases and insect pests of the sympodial bamboos in No. 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 1.8 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 nodes of the branch with the stump are left, the length of the branch is 50m, no leaves are left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: pre-soaking the stump part of the branch with the branch sheaths in 50mg/L rooting powder aqueous solution for 2h, then placing the branch in clean water at the constant temperature of 20 ℃, vertically placing the branch with the stump part 5cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 20 ℃, air is kept to be filled continuously, and water is changed for 1 time every 3 days.
Comparative example 3
(1) Selecting branches: selecting a 2-year-old main branch without diseases and insect pests of the sympodial bamboos in No. 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 1.8 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 stump-carrying branches are left, the length of the branches is 50cm, no leaves are left, the branch stump part and branch sheaths are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: pre-soaking the stump part of the branch with the branch sheaths in 50mg/L rooting powder aqueous solution for 2h, then placing the branch in clean water at the constant temperature of 20 ℃, vertically placing the branch, keeping the stump part of the branch at the constant temperature of the water by using an automatic water temperature controller, and keeping the water at the constant temperature;
(4) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 22 ℃, and water is changed for 1 time every 3 days.
Comparative example 4
(1) Selecting branches: selecting a 2-year-old main branch without diseases and insect pests of the sympodial bamboos in No. 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 1.8 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 nodes of the branch with the stump are left, the length of the branch is 50cm, no leaves are left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: placing the branch stump part stripped with the branch sheaths in clean water at the constant temperature of 20 ℃, vertically placing the branches, keeping the branch stump part 12cm away from the water surface, and keeping the water body at the constant temperature through an automatic water temperature controller;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage is carried out, direct sunlight is avoided, the temperature of clear water is kept at 20 ℃, air is kept to be filled continuously, and water is changed for 1 time every 3 days.
Comparative example 5
(1) Selecting branches: selecting a 2-year-old main branch without diseases and insect pests of the sympodial bamboos in No. 1 month, wherein the main branch is 1 year old and the diameter of a first section at the base part of the main branch is 1.8 cm;
(2) branch collection: cutting off the joint of the mother bamboo stalk and the main branch stump to obtain a main branch, wherein the branch stump of the collected main branch is not cracked, stump buds are not damaged, the main branch is cut off at the tip part, 2 nodes of the branch with the stump are left, the length of the branch is 50cm, no leaves are left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing treatment is carried out;
(3) controlling water temperature: pre-soaking a 50mg/L rooting powder aqueous solution in the stump part of the branch with the branch sheaths in a normal-temperature clear water for 2 hours, vertically placing the branch, and keeping the stump part of the branch 12cm away from the water surface;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: indoor cuttage, direct sunlight prevention, normal-temperature culture, continuous air filling, and water change for 1 time every 3 days.
The difference between comparative example 1 and example 1 is: the diameter of the first section of the base of the main branch in step (1) in comparative example 1 was 1.7 cm.
The difference between comparative example 2 and example 1 is: the stump of the branch in the step (3) of the comparative example 2 is 5cm away from the water surface.
The difference between comparative example 3 and example 1 is: comparative example 3 was not aerated and oxygenated.
The difference between comparative example 4 and example 1 is: comparative example 4 did not use the aqueous rooting powder solution for pretreatment.
The difference between comparative example 5 and example 1 is: the culture was carried out at room temperature in clear water without constant temperature in comparative example 5.
10 repetitions of the main branches and the secondary branches in the comparative examples and the examples were selected, and the rooting time per branch, the number of roots per branch, the length of root per branch, the number of capillary roots grown and the rooting rate were recorded. The results are shown in Table 1:
TABLE 2 test results of examples 1 to 4 and comparative examples 1 to 5
As shown in Table 1, the example 1-4 roots after the stump parts of the branches are cultured for 3 days in constant temperature clear water, the rooting time is shortened compared with the comparative example 1-5, and the number of roots of each branch of the example 1-4 is more than that of the comparative example 1-5; examples 1-4 each shoot had a longer rooted length than comparative examples 1-5; the number of growing capillary roots of examples 1 to 4 was greater than that of comparative examples 1 to 5; the rooting rate of the branches in the examples 1 to 4 is higher than that in the comparative examples 1 to 5, wherein the rooting rate of the branches in the example 3 is up to 72.7 percent, and the rooting rate of the sympodial bamboos is the highest under the condition of constant temperature of 22 ℃ clear water hydroponics; comparative example 5 does not culture in constant temperature clear water, the rooting time is 10 days, the rooting rate is only 22.3%, which shows that the constant temperature clear water culture plays an important role.
Application example
Taking 10 main branches of the flower punt-pole bamboo, the caragana bambusicola, the large green bamboo, the strong green bamboo, the flower garcinia mangostana, the meinong dendrocalamus latiflorus, the Malaytea dragon bamboo and the Vietnam giant bamboo, and carrying out the test by adopting the technical scheme of the embodiment 1, wherein the results are shown in a table 2:
TABLE 2 rooting of various sympodial bamboos under the conditions of example 1
The technical scheme of the embodiment 1 is adopted, and the chrysanthemum setosum bamboo, the malachite, the large green bamboo, the strong green bamboo, the flower garcinia mangostana, the phyllostachys pubescens, the meinong dendrocalamus, the dendrocalamus latiflorus and the Vietnam giant bamboo start to take root in the 3 rd day, and the rooting rate is more than 50%.
Claims (9)
1. A method for rapidly rooting sympodial bamboo branches is characterized by comprising the following steps:
(1) selecting branches: selecting a main branch or a secondary branch of the sympodial bamboos, wherein the diameter of a first section of the base of the main branch or the secondary branch is more than 1.8 cm;
(2) branch collection: cutting off a joint of a mother bamboo stalk and a main branch stump to obtain a main branch, cutting off a joint of the main branch stump and a secondary branch stump to obtain a secondary branch, cutting off tips of the main branch and the secondary branch to leave 2-3 branches with stumps, the length of the branches is 50-55cm, leaves are not left, branch sheaths of the branch stump part are peeled off after collection, and shading and moisturizing are carried out;
(3) controlling water temperature: pretreating the stump part of the branch with the branch sheaths, and then placing the branch stump part in clean water at the constant temperature of 20-26 ℃, wherein the distance between the stump part of the branch and the water surface is 10-12 cm;
(4) filling air and oxygenating: filling air into the clean water in the step (3);
(5) and (3) cutting management: avoiding direct irradiation of sunlight, keeping the temperature of clear water at 20-26 ℃, keeping continuously filling air, and changing water 1 time every 3-4 days.
2. The method according to claim 1, wherein said shoots in step (1) are selected from 1-2 months.
3. The method according to claim 1, wherein the sympodial bamboos in step (1) are grown for more than 2 years and have no plant diseases and insect pests.
4. The method according to claim 1, wherein the shoot stump of the shoot harvested in step (2) is not cracked and the stump bud is not damaged.
5. The method according to claim 1, wherein the placement of the branches in step (3) is vertical or diagonal.
6. The method for rapid rooting of clump bamboo shoots according to claim 1, wherein the pretreatment in step (3) is to soak the root parts of the shoots with an aqueous solution of rooting powder with a concentration of 50-100mg/L for 2-3 h.
7. The method for rapid rooting of sympodial bamboo shoots according to claim 1, wherein the temperature of the constant temperature water in step (3) is 22 ℃.
8. The use of the method of rapid rooting of sympodial bamboo shoots according to any one of claims 1-7 in seedling raising and forestation of sympodial bamboo.
9. The use of claim 8, wherein the sympodial bamboo is selected from the group consisting of tamarind, horse chestnut, DALVZHU, SUZUOZHU, ZHUANGLUOZHU, HUASHUANGZHU, MEISONGMA, MAIMITANGLONG, and YUNNANDAZHU.
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CN202011318823.0A CN112400502A (en) | 2020-11-23 | 2020-11-23 | Method for rapidly rooting sympodial bamboo branches and application thereof |
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CN109169226A (en) * | 2018-09-29 | 2019-01-11 | 浙江省亚热带作物研究所 | A kind of ciltivating process of bamboo |
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CN109169226A (en) * | 2018-09-29 | 2019-01-11 | 浙江省亚热带作物研究所 | A kind of ciltivating process of bamboo |
CN109757233A (en) * | 2019-03-18 | 2019-05-17 | 中国林业科学研究院亚热带林业研究所 | A kind of Cluster Bamboo full exposure is sprayed cuttage rapid propagating method |
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