CN112378887A - Analysis method of blood coagulation analyzer and blood coagulation analyzer - Google Patents
Analysis method of blood coagulation analyzer and blood coagulation analyzer Download PDFInfo
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- 230000023555 blood coagulation Effects 0.000 title claims abstract description 25
- 238000004458 analytical method Methods 0.000 title claims abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 170
- 230000003287 optical effect Effects 0.000 claims abstract description 106
- 238000000034 method Methods 0.000 claims abstract description 50
- 230000005540 biological transmission Effects 0.000 claims abstract description 47
- 239000008280 blood Substances 0.000 claims abstract description 40
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000011324 bead Substances 0.000 claims abstract description 26
- 230000002159 abnormal effect Effects 0.000 claims abstract description 20
- 230000015271 coagulation Effects 0.000 claims abstract description 17
- 238000005345 coagulation Methods 0.000 claims abstract description 17
- 238000011049 filling Methods 0.000 claims description 57
- 239000003153 chemical reaction reagent Substances 0.000 claims description 46
- 238000011068 loading method Methods 0.000 claims description 19
- 230000037452 priming Effects 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 11
- 230000035931 haemagglutination Effects 0.000 claims description 10
- 238000003860 storage Methods 0.000 claims description 9
- 239000003593 chromogenic compound Substances 0.000 claims description 8
- 230000005856 abnormality Effects 0.000 claims description 4
- 230000007246 mechanism Effects 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims 1
- 238000000265 homogenisation Methods 0.000 claims 1
- 238000000703 high-speed centrifugation Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 19
- 239000013307 optical fiber Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 208000007536 Thrombosis Diseases 0.000 description 4
- 230000023597 hemostasis Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000790 scattering method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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Abstract
The embodiment of the invention is suitable for the technical field of detection equipment, and provides an analysis method of a blood coagulation analyzer and the blood coagulation analyzer, which comprises the following steps: at least one optical transmission channel with a first wavelength, and monochromatic light with the first wavelength is formed by the channel light source and the optical filter; at least one optical transmission channel with a second wavelength, and monochromatic light with the second wavelength is formed by the channel light source and the optical filter; at least one coagulation item, by default, using said first wavelength, and switching to an optical transmission channel of said second wavelength if an anomaly in the signal of said first wavelength is found; most abnormal samples of the project can be detected in time without high-speed centrifugation, blood collection again or recourse to a magnetic bead method with relatively higher detection cost; meanwhile, instrument complexity and relatively higher instrument cost caused by integrating a plurality of detection wavelengths in the same detection channel are avoided.
Description
Technical Field
The invention belongs to the technical field of detection equipment, and particularly relates to an analysis method of a blood coagulation analyzer and the blood coagulation analyzer.
Background
The blood coagulation analyzer is a special instrument for analyzing thrombus and hemostasis, can detect various thrombus and hemostasis indexes, provides valuable indexes for diagnosis of bleeding and thrombotic diseases, monitoring of thrombolysis and anticoagulation treatment and observation of curative effects, and is the most basic equipment used in a thrombus/hemostasis laboratory at present.
The full-automatic blood coagulation analyzer can support the detection project of the coagulation method. On the basis of the items of detection by the coagulation method, a partial hemagglutination analyzer can also support the items of detection by the immunoturbidimetry and/or chromogenic substrate method.
Two basic detection principles of the coagulation method are the optical method and the magnetic bead method. Compared with an optical method, the magnetic bead method is hardly influenced by abnormal interference substances in the sample, and results can be given to the abnormal sample in time. However, instrument cost and detection cost of the magnetic bead method instrument are generally higher than those of the optical method.
In the prior art, a blood coagulation analyzer based on an optical principle for a partial coagulation detection project adopts a mode of integrating a plurality of wavelengths of detection light at the same detection position, and reduces the interference of a sample aiming at the partial coagulation detection project. In such instruments, the dominant wavelength is selected by default, and is typically the wavelength at which the detection signal has a larger amplitude of change, which is more favorable for the detection sensitivity of the sample. However, when the sample has interfering substances, signals with different wavelengths are affected differently, and if it is determined that the interference on the main wavelength is large, the instrument can automatically switch to the sub-wavelength with the relatively small interference degree of the detection channel for detection.
Because the automatic blood coagulation detection instrument has a plurality of detection positions, part of products adopt a light source + optical fiber light splitting mode to provide multi-wavelength detection light for each detection position. The optical fiber splitting mode brings about the complexity of the instrument and the relatively higher cost of the optical system.
If one LED light source is used for each detection position, the cost of the instrument is relatively lower. If the instrument has at least two detection channels with different wavelengths, the detection channels with different wavelengths can be switched, so that the abnormal interference sample can be detected in time without high-speed centrifugation, blood collection again or recourse to a magnetic bead method with relatively higher detection cost. While the instrument maintains low manufacturing costs.
Disclosure of Invention
The embodiment of the invention provides an analysis method of a blood coagulation analyzer and the blood coagulation analyzer, aiming at solving the problem of low detection efficiency in the prior art.
The embodiment of the invention is realized in such a way that the analysis method of the blood coagulation analyzer comprises the following steps:
at least one optical transmission channel with a first wavelength, and monochromatic light with the first wavelength is formed by the channel light source and the optical filter;
at least one optical transmission channel with a second wavelength, and monochromatic light with the second wavelength is formed by the channel light source and the optical filter;
at least one coagulation item, by default, using said first wavelength, and switching to an optical transmission channel of said second wavelength if an anomaly in the signal of said first wavelength is found.
Further, if the signal of the first wavelength is found to be abnormal, the same cuvette is switched to the optical transmission channel of the second wavelength.
Further, if the signal of the first wavelength is found to be abnormal, another cuvette is switched to the optical transmission channel of the second wavelength.
Furthermore, if the signals of the first wavelength and the second wavelength are found to be abnormal, the magnetic bead reaction cup is moved to the detection position of the magnetic bead method for detection.
The present invention also provides a blood coagulation analyzer comprising: the device comprises a rack, a movable filling assembly arranged on the rack, a movable gripper arranged on the rack, a detection assembly arranged on the rack, a reagent storage device, a sample loading device and a reaction cup supply assembly;
the detection assembly comprises: a first transmissive optical detection member disposed on the frame for performing coagulation detection at a first wavelength, and a second transmissive optical detection member disposed on the frame for performing coagulation detection at a second wavelength.
Still further, the first transmissive optical detection member includes: the LED light source comprises a plurality of first detection positions arranged on the rack, first LED lamps correspondingly arranged on two sides of each first detection position, a first photoelectric sensor and a first optical filter, wherein the first LED lamps and the first optical filters generate a first wavelength.
Still further, the second transmissive optical detection member includes: the LED light source comprises a plurality of second detection positions arranged on the rack, and a second LED lamp, a second photoelectric sensor and a second optical filter which are correspondingly arranged on two sides of each second detection position, wherein the second LED lamp and the second optical filter generate a second wavelength.
Still further, the detection assembly includes: and a third transmission optical detection component which is arranged on the frame and performs immunoturbidimetry detection.
Still further, the detection assembly includes: and a fourth transmission optical detection member disposed on the frame for detecting by a chromogenic substrate method.
Further, the first transmissive optical member, the second transmissive optical member, a part or the whole can be reused for detection by immunoturbidimetry or chromogenic substrate method.
Still further, the mobile priming assembly is used for both reagent priming and blood sample priming. The method comprises the following steps: the double-purpose filling device comprises a first three-dimensional moving part arranged on the rack, a first suction control part connected with the first three-dimensional moving part, and a double-purpose filling needle connected with the first suction control part.
Still further, the mobile priming assembly comprises a reagent priming member and a blood sample priming member;
the reagent filling member includes: a second three-dimensional moving member disposed on the frame, a second aspiration control member connected to the second three-dimensional moving member, and a reagent filling needle connected to the second aspiration control member;
the blood sample filling member comprises: the blood sample feeding device comprises a third three-dimensional moving member arranged on the rack, a third suction control member connected with the third three-dimensional moving member, and a blood sample feeding needle connected with the third suction control member.
Still further, the detection assembly further comprises: and the magnetic bead method detection channel is arranged on the rack.
Furthermore, the removal tongs has the function of mixing, and the removal tongs includes: the reaction cup blending mechanism comprises a fourth three-dimensional moving member arranged on the rack, a hand grip structure connected with the fourth three-dimensional moving member, and a reaction cup blending structure arranged on the hand grip structure.
Compared with the prior art, in the embodiment of the invention, the channel light source and the optical filter form monochromatic light with the first wavelength through at least one optical transmission method channel with the first wavelength; at least one optical transmission channel with a second wavelength, and monochromatic light with the second wavelength is formed by the channel light source and the optical filter; by utilizing at least one coagulation method project, the first wavelength is adopted by default, and if the signal of the first wavelength is found to be abnormal, the optical transmission method channel is switched to the optical transmission method channel of the second wavelength, so that the sample has good advantages in retesting and sample emergency treatment, the detection efficiency of the hemagglutination analyzer can be improved, and the safety is high; the transmission method has the advantages of small occupied space, compact instrument layout and the like compared with the scattering method; meanwhile, the mode of one light source at each detection position is adopted, so that the detection pertinence is strong, and the cost of the instrument is relatively lower.
Drawings
FIG. 1 is a schematic view of the overall structure of a blood coagulation analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic view of the overall structure of a blood coagulation analyzer according to an embodiment of the present invention;
FIG. 3 is a schematic view of the overall structure of a blood coagulation analyzer according to an embodiment of the present invention;
FIG. 4 is a schematic view of the overall structure of a blood coagulation analyzer according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a first transmissive optical detection member provided in an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a second transmissive optical detection member provided in an embodiment of the present invention;
FIG. 7 is a schematic diagram of the overall structure of a mobile dispensing assembly provided by an embodiment of the present invention;
FIG. 8 is a schematic diagram of the overall structure of a reagent filling member provided in an embodiment of the present invention;
FIG. 9 is a schematic view of the overall structure of a blood sample filling member provided by an embodiment of the present invention;
FIG. 10 is a schematic view of the overall structure of a cuvette supply unit according to an embodiment of the present invention;
FIG. 11 is a schematic diagram of the overall structure of the moving gripper provided by the embodiment of the present invention;
FIG. 12 is a schematic structural view of a first transmissive optical detection member provided in another embodiment of the present invention;
fig. 13 is a schematic structural view of a second transmissive optical detection member according to another embodiment of the present invention.
Wherein, 1, a frame, 2, a mobile filling component, 21, a first three-dimensional moving component, 22, a first suction control component, 23, a dual-purpose filling needle, 3, a mobile gripper, 4, a reagent storage device, 5, a reaction cup supply component, 51, a reaction cup loading storage area, 52, a conveying track, 53, a reaction cup dispatching component, 6, a detection component, 61, a first transmission optical detection component, 611, a first detection position, 612, a first LED lamp, 613, a first photoelectric sensor, 62, a second transmission optical detection component, 621, a second detection position, 622, a second LED lamp, 623, a second photoelectric sensor, 63, a first optical filter, 64, a second optical filter, 7, a sample loading device, 71, a sample rack, 72, a sample fixing position, 73, a turntable, 74, a sample tube placing position, 75, a sliding rail, 76, a tray filling component, 8, a reagent component, 81. the device comprises a second three-dimensional moving part, a second suction control part, a reagent filling needle, a reagent filling member, a blood sample filling member, a reagent filling needle, a reagent filling member.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides an analysis method of a blood coagulation analyzer, which comprises the following steps: at least one optical transmission channel with a first wavelength, and monochromatic light with the first wavelength is formed by the channel light source and the optical filter; at least one optical transmission channel with a second wavelength, and monochromatic light with the second wavelength is formed by the channel light source and the optical filter; at least one coagulation item, by default, using said first wavelength, and switching to an optical transmission channel of said second wavelength if an anomaly in the signal of said first wavelength is found.
Specifically, a first wavelength of monochromatic light is formed on at least one optical transmission channel and filter of a first wavelength, and a second wavelength of monochromatic light is formed on at least one optical transmission channel and filter of a second wavelength. By default, using the first wavelength, if the signal of the first wavelength is found to be abnormal, the optical transmission channel of the second wavelength is switched to detect. If the signal of the second wavelength is abnormal, the signal can be detected by switching to the first wavelength. The channels with various wavelengths can improve the detection efficiency and are widely used. Therefore, most abnormal samples of the project can be detected in time without high-speed centrifugation, blood re-sampling or recourse to a magnetic bead method with relatively higher detection cost; meanwhile, instrument complexity and relatively higher instrument cost caused by integrating a plurality of detection wavelengths in the same detection channel are avoided.
In this embodiment, if the signal of the first wavelength is found to be abnormal, the same cuvette is switched to the optical transmission channel of the second wavelength. If the signal with the first wavelength is found to be abnormal, the reaction cup originally placed on the detection position on the optical transmission method channel with the first wavelength can be grabbed to the optical transmission method channel with the second wavelength by moving the grabbing hand to detect, the reaction cup switching detection effect is good, and the detection efficiency is higher.
In this embodiment, if the signal of the first wavelength is found to be abnormal, another cuvette is switched to the optical transmission channel of the second wavelength. If the signal with the first wavelength is found to be abnormal, the reaction cup originally placed on the detection position on the optical transmission method channel with the first wavelength can be grabbed to the optical transmission method channel with the first wavelength by moving the grabbing hand to detect, the reaction cup switching detection effect is good, and the detection efficiency is higher.
In this embodiment, if the signal abnormality of the first wavelength and the signal abnormality of the second wavelength are found, the magnetic bead cuvette is moved to the detection site of the magnetic bead method, and detection is performed. If the signals of the first wavelength and the second wavelength are found to be abnormal, the magnetic bead reaction cup can be moved to the detection position of the magnetic bead method for detection. The detection mode is various, and the detection effect is better.
Alternatively, for example, the first wavelength is 405nm, the second wavelength is 660nm, and the other wavelengths are 850 nn. If an abnormal sample happens and cannot be tested due to interference during detection at 405nm, the sample can be switched to a transmission method channel with a second wavelength for detection, and if the signals of the first wavelength and the second wavelength are found to be abnormal, the magnetic bead reaction cup is moved to a magnetic bead method detection position for detection. If the signals of the first wavelength and the second wavelength are found to be abnormal, the magnetic bead reaction cup can be moved to the detection position of the magnetic bead method for detection. The detection mode is various, and the detection effect is better.
An embodiment of the present invention further provides a blood coagulation analyzer, as shown in fig. 1 to 4, including: the device comprises a rack 1, a movable filling assembly 2 arranged on the rack 1, a movable gripper 3 arranged on the rack 1, a detection assembly 6 arranged on the rack 1, a reagent storage device 4, a sample loading device 7 and a reaction cup supply assembly 5; the detection assembly 6 includes: a first transmissive optical detection member 61 provided on the gantry 1 for performing a coagulation detection by a first wavelength, and a second transmissive optical detection member 62 provided on the gantry 1 for performing a coagulation detection by a second wavelength. The reagent storage device 4 is used for storing reagents, the sample loading device 7 is used for loading samples, and the reaction cup supply assembly 5 is used for supplying reaction cups, so that the reagents and blood samples can be conveniently filled into the reaction cups by moving the filling assembly 2.
Specifically, the movable filling assembly 2 arranged on the rack 1 is used for filling a sample and a reagent into a reaction cup, the movable gripper 3 is used for uniformly mixing the sample and the reagent and grabbing the reaction cup to the detection assembly 6 for detection, the first transmission optical detection component 61 on the detection assembly 6 is used for carrying out solidification detection through a first wavelength, the second transmission optical detection component 62 is used for carrying out solidification detection through a second wavelength, the detection wavelength is strong in pertinence, the detection effect is good, the detection efficiency of the sample is improved, and the application is wide.
In an alternative embodiment of the present invention, as shown in fig. 5, the first transmissive optical detection member 61 includes: the first light-emitting diode (LED) light source comprises a plurality of first detection positions 611 arranged on a rack 1, a first LED light 612 correspondingly arranged on two sides of each first detection position 611, a first photoelectric sensor 613 and a first optical filter 63, wherein the first LED light 612 and the first optical filter 63 generate a first wavelength.
Specifically, the reaction cup is placed on the first detection position 611 by moving the hand grip 3, the first wavelength light emitted by the first LED lamp 612 passes through the reaction cup and is transmitted to the first photoelectric sensor 613, the optical signal of the first wavelength is converted into an electrical signal by the photoelectric sensor, the electrical signal is transmitted to the processor, and data is displayed on the display screen. The reaction cup is conveniently installed at the first detection position 611, the light emitting effect of the first LED lamp 612 is good, and electric energy is saved. The first photo-sensor 613 senses the light with the first wavelength, thereby improving the detection efficiency of the first detection bit 611 on the sample. The blood sample under special conditions can be detected without replacing the device, and the first LED lamps 612 and the first photo-sensors 613 are in one-to-one correspondence, which can save the cost compared with the method of adding optical filters through optical fibers.
In an alternative embodiment of the present invention, as shown in fig. 12, the cuvette is placed on the first detection position 611 by moving the hand grip 3, the light with the first wavelength emitted by the first LED lamp 612 passes through the first optical filter 63 and then is transmitted to the first photoelectric sensor 613 through the cuvette, the optical signal with the first wavelength is converted into an electrical signal by the photoelectric sensor, and the electrical signal is transmitted to the processor for displaying data through the display screen. The reaction cup is conveniently installed at the first detection position 611, the light emitting effect of the first LED lamp 612 is good, and electric energy is saved. The first photo-sensor 613 senses the light with the first wavelength, thereby improving the detection efficiency of the first detection bit 611 on the sample. The blood sample under special conditions can be detected without replacing the device, and the first LED lamps 612 and the first photo-sensors 613 are in one-to-one correspondence, which can save the cost compared with the method of adding optical filters through optical fibers.
In the present embodiment, as shown in fig. 6, the second transmissive optical detection member 62 includes: the second detection positions 621 arranged on the rack 1, the second LED lamps 622, the second photoelectric sensors 623 and the second optical filters 64 correspondingly arranged on two sides of each second detection position 621, and the second LED lamps 622 and the second optical filters 64 generate a second wavelength.
Specifically, the reaction cup is placed on the second detection position 621 by moving the hand grip 3, the second wavelength light emitted by the second LED lamp 622 passes through the reaction cup and is transmitted to the second photoelectric sensor 623, the optical signal with the second wavelength is converted into an electrical signal by the photoelectric sensor, the electrical signal is transmitted to the processor, and data is displayed through the display screen. The reaction cup is conveniently installed at the second detection position 621, the light emitting effect of the second LED lamp 622 is good, and electric energy is saved. The second photoelectric sensor 623 senses light with a second wavelength, so that the detection efficiency of the second detection position 621 on the sample is improved, and the use is convenient. The blood sample under special conditions can be detected without replacing the device, and the cost can be saved in a one-to-one correspondence manner of the second LED lamps 622 and the second photoelectric sensors 623 compared with a manner of adding optical filters through optical fibers.
Further, a first filter 63 is disposed between the first LED lamp 612 and the first photo sensor 613; a second optical filter 64 is further disposed between the second LED lamp 622 and the second photoelectric sensor 623. The light with the wavelength different from the first wavelength can be filtered through the first optical filter 63, and the light with the wavelength different from the second wavelength can be filtered through the second optical filter 64, so that the first photoelectric sensor 613 and the second photoelectric sensor 623 can receive the corresponding light with the first wavelength and the second wavelength, the pertinence is strong, the interference is avoided, the effect of converting the optical signal into the electrical signal is good, the accuracy is high, and the adaptation is wide.
In an alternative embodiment of the present invention, as shown in fig. 13, the cuvette is placed on the second detection position 621 by moving the hand grip 3, the light with the second wavelength emitted by the second LED lamp 622 passes through the second optical filter 64 and then is transmitted to the second photo sensor 623 through the cuvette, the optical signal with the second wavelength is converted into an electrical signal by the photo sensor, and the electrical signal is transmitted to the processor, and the data is displayed by the display screen. The reaction cup is conveniently installed at the second detection position 621, the light emitting effect of the second LED lamp 622 is good, and electric energy is saved. The second photoelectric sensor 623 senses light with a second wavelength, so that the detection efficiency of the second detection position 621 on the sample is improved, and the use is convenient. The blood sample under special conditions can be detected without replacing the device, and the cost can be saved in a one-to-one correspondence manner of the second LED lamps 622 and the second photoelectric sensors 623 compared with a manner of adding optical filters through optical fibers.
In the present embodiment, as shown in fig. 1, the detecting unit 6 includes: and a third transmission optical detection component which is arranged on the frame 1 and performs immunoturbidimetric detection. The reaction cup is convenient to be placed on the third transmission optical detection component with immunoturbidimetry detection for detection, and the detection effect is better.
In the present embodiment, as shown in fig. 1, the detecting unit 6 includes: and a fourth transmission optical detection member disposed on the frame 1 and used for detection by a chromogenic substrate method. The reaction cup is conveniently placed on the fourth transmission optical detection component for detection by the chromogenic substrate method, and the detection effect is better.
In this embodiment, the detecting component 6 further includes: and the magnetic bead method detection channel is arranged on the frame 1. The magnetic bead measurement position 15 is arranged on the magnetic bead method detection channel, so that magnetic bead method detection is conveniently carried out on the reaction cup, and the detection effect is better.
In this embodiment, as shown in FIG. 1, a part or all of the first transmission optical member 61 and the second transmission optical member 62 may be reused for the detection by immunoturbidimetry or chromogenic substrate method. The detection function is diversified, the detection efficiency is high, and the application is wide.
In this embodiment, as shown in fig. 1, a plurality of the first detection bits 611 are arranged on the same straight line. The arrangement of a plurality of first detection positions 611 can be optimized, and the use is convenient.
In this embodiment, as shown in fig. 1 and fig. 5 to 6, a plurality of the second detection bits 621 are arranged on the same straight line. The arrangement of the second detection positions 621 can be optimized, and the use is convenient.
In this embodiment, as shown in fig. 1 and fig. 5 to 6, a plurality of the first detection bits 611 and a plurality of the second detection bits 621 are arranged on the same straight line. Optimize and arrange, reduce required space of arranging, and the test tube that the machinery of being convenient for grabbed and will fail to detect grabs another detection zone.
In an alternative embodiment of the invention, as shown in fig. 1 and 7, the mobile dispensing assembly 2 comprises: a first three-dimensional moving member 21 provided on said frame 1, a first suction control member 22 connected to said first three-dimensional moving member 21, and a dual-purpose filling needle 23 connected to said first suction control member 22.
Specifically, the dual-purpose filling needle 23 is used for extracting a sample and a reagent from the sample and filling the sample into the reaction cup, the first suction control part 22 is used for controlling the suction function of the dual-purpose filling needle 23, and the first three-dimensional moving part 21 is used for moving the dual-purpose filling needle 23 among the reagent storage device 4, the sample loading device 7 and the moving hand grip 3, so that the reagent and sample filling is facilitated.
Because first three-dimensional moving member 21 sets up on frame 1, first suction control part 22 is connected on first three-dimensional moving member 21, and double-purpose filling needle 23 sets up on first suction control part 22, controls the suction volume of double-purpose filling needle 23 through first suction control part 22, removes the filling through first three-dimensional moving member 21, and double-purpose filling needle 23 is various, and filling efficiency is high, practices thrift the cost.
In an alternative embodiment of the present invention, as shown in fig. 1, 8-9, said mobile priming assembly 2 comprises a reagent priming member 8 and a blood sample priming member 9; the reagent filling means 8 comprises: a second three-dimensional moving member 81 provided on the frame 1, a second suction control member 82 connected to the second three-dimensional moving member 81, and a reagent filling needle 83 connected to the second suction control member 82; said blood sample filling means 9 comprises: a third three-dimensional moving member 81 provided on said frame 1, a third suction control member 92 connected to said third three-dimensional moving member 81, and a blood sample filling needle 93 connected to said third suction control member 92.
Specifically, the reagent filling component 8 is used for reagent extraction and filling, the blood sample filling component 9 is used for blood extraction and filling, and blood samples and reagents are respectively extracted and filled into the reaction cups, so that the filling needle is cleaner and tidier, the detection precision is high, and the use is convenient.
Specifically, the reagent filling needle 83 is inserted into the reagent storage device 4, the second suction control member 82 controls the amount of reagent sucked by the reagent filling needle 83, and after reagent suction is completed, the reagent filling needle 83 is moved to the reaction cup by the second three-dimensional moving member 81 to fill the reagent.
Specifically, insert blood sample filling needle 93 in sample loading device 7, through the volume of the suction of third suction control spare 92 control blood sample filling needle 93 to the blood sample size, treat the blood sample suction and accomplish the back, move blood sample filling needle 93 to the reaction cup through third three-dimensional moving member 81 on, carry out the blood sample filling for blood sample and reagent carry out mixing reaction, and then improve reaction efficiency, the result of use is better.
In an alternative embodiment of the invention, as shown in fig. 1 and 11, the moving gripper 3 comprises: the device comprises a fourth three-dimensional moving member 11 arranged on the rack 1, a hand grab structure 12 connected with the fourth three-dimensional moving member 11, and a reaction cup blending structure 13 arranged on the hand grab structure 12.
Specifically, move through fourth three-dimensional moving member 11 and correspond tongs structure 12 on the reaction cup, snatch the reaction cup after will annotating blood sample and reagent through tongs structure 12 to carry out blood sample and reagent mixing through reaction cup mixing structure 13, wait blood sample and reagent mixing after, move the reaction cup through fourth three-dimensional moving member 11 and carry out sample measurement on detecting element 6. The gripping handle structure 12 is convenient to grip or loosen, facilitates gripping of reaction cups and placing of the reaction cups, is stable in gripping and is convenient to use.
In an alternative embodiment of the present invention, as shown in fig. 1, the sample loading device 7 is a plurality of sample holders 71 detachably disposed on the rack 1, and the sample holders 71 are provided with a plurality of sample fixing positions 72.
Specifically, by detachably mounting the sample holder 71 on the rack 1, the fixing effect is good, and meanwhile, the replacement or maintenance is facilitated. And sample frame 71 is used for setting up a plurality of sample fixed position 72, and through putting the sample into sample fixed position 72 and carry out fixed mounting, the sample is fixed effectual, and it is convenient to detect, and the accuracy is high.
Optionally, as shown in fig. 2 to 3, the plurality of sample fixing positions 72 are arranged side by side or in parallel, so that the sample is conveniently loaded and the use efficiency is high.
In an alternative embodiment of the present invention, as shown in fig. 1, the rack 1 is provided with a slide rail 75, and the sample rack 71 is slidably disposed on the slide rail 75.
Specifically, through set up slide rail 75 in frame 1, slide sample frame 71 and set up on slide rail 75, be convenient for slide sample frame 71 to can change the sample in a plurality of sample fixed position 72 on sample frame 71, the adaptation is extensive.
In an alternative embodiment of the present invention, as shown in fig. 2, the sample loading device 7 is a tray assembly 76 that is integrally movable on the rack 1, the tray assembly 76 is provided with a plurality of sample tube placement positions 74, the sample tubes are placed in the tray assembly 76, and after the tray assembly 76 is set in position relative to the rack 1, each sample tube has a fixed coordinate position relative to the rack 1.
In an alternative embodiment of the present invention, as shown in fig. 4, the sample loading device 7 is a turntable 73 disposed on the rack 1, and the turntable 73 is provided with a plurality of sample fixing positions 72.
Specifically, by setting the sample loading device 7 as the rotary disk 73, the rotary disk 73 is provided with a plurality of sample fixing positions 72, so that the sample can be conveniently loaded in the plurality of sample fixing positions 72 for fixing. Since the turntable 73 is convenient to rotate to adjust the position of the sample fixing position 72, the sample moves along with the sample fixing position 72 during the movement. When the filling assembly 2 is moved to completely extract the sample, the rotary disc 73 is rotated to extract and fill the rest of the sample into the reaction cup, so that the sample replacement effect is high, and the use is convenient.
In an alternative embodiment of the present invention, as shown in fig. 3, the sample loading device 7 is provided with a plurality of fixed sample tube placement positions 74 on the rack 1. The sample tube is convenient to install and use, and the sample can be placed in the sample tube.
In an alternative embodiment of the present invention, as shown in fig. 1 and 10, the cuvette supply unit 5 includes: the reaction cup loading and storing device comprises a reaction cup loading and storing area 51, a conveying track 52 and a reaction cup scheduling part 53, wherein the input end of the conveying track 52 corresponds to the reaction cup loading and storing area 51, and the output end of the conveying track 52 corresponds to the reaction cup scheduling part 53.
Specifically, the cuvettes are adjusted by the cuvette adjusting member by placing the cuvettes in the cuvette loading/storing section 51 and transferring the cuvettes to the cuvette adjusting member through the transfer rail 52. The input end of the conveying track 52 corresponds to the reaction cup loading storage area 51, and the output end of the conveying track 52 corresponds to the reaction cup scheduling part 53, so that the reaction cup conveying effect is good, the efficiency is high, and the use is convenient.
In an alternative embodiment of the present invention, as shown in FIGS. 1-4, there is also a separate cuvette temperature incubation assembly 10.
Specifically, the mixed reagent and the blood sample are incubated through the independent reaction cup temperature incubation device 10, so that the reaction efficiency between the reagent and the blood sample is higher, and the reaction effect is better.
Further, the reaction temperature incubation device 10 can be arranged at the same position as the reaction cup measuring position 61, and the reaction cup measuring position 61 is used for measuring after the incubation of the reagent and the blood sample is finished by performing the incubation work at first, so that the space is saved.
Optionally, the device also comprises a dual-magnetic-circuit magnetic bead method measuring device of a coagulation method, and the magnetic bead method measuring device has a good blood sample measuring effect.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (14)
1. An analysis method of a blood coagulation analyzer, comprising the steps of:
at least one optical transmission channel with a first wavelength, and monochromatic light with the first wavelength is formed by the channel light source and the optical filter;
at least one optical transmission channel with a second wavelength, and monochromatic light with the second wavelength is formed by the channel light source and the optical filter;
at least one coagulation item, by default, using said first wavelength, and switching to an optical transmission channel of said second wavelength if an anomaly in the signal of said first wavelength is found.
2. The method of claim 1, wherein the abnormality of the signal of the first wavelength is detected by switching the same cuvette to the optical transmission channel of the second wavelength.
3. The method of claim 1, wherein if the signal of the first wavelength is found to be abnormal, the other cuvette is switched to the optical transmission channel of the second wavelength.
4. The method of analyzing a blood coagulation analyzer according to claim 1, wherein when the signal abnormality is found in the first wavelength and the second wavelength, the magnetic bead cuvette is moved to a detection site of a magnetic bead method to perform detection.
5. A blood clotting analyzer, comprising: the device comprises a rack, a movable filling component arranged on the rack, a movable gripper arranged on the rack, a detection component arranged on the rack, a reagent storage device, a sample loading device and a reaction cup supply component;
the detection assembly comprises: a first transmissive optical detection member disposed on the frame for performing coagulation detection at a first wavelength, and a second transmissive optical detection member disposed on the frame for performing coagulation detection at a second wavelength.
6. The hemagglutination analyzer of claim 5, wherein the first transmission optical detection member comprises: the LED light source comprises a plurality of first detection positions arranged on the rack, first LED lamps correspondingly arranged on two sides of each first detection position, a first photoelectric sensor and a first optical filter, wherein the first LED lamps and the first optical filters generate a first wavelength.
7. The hemagglutination analyzer of claim 6, wherein the second transmission optical detection member comprises: the LED light source comprises a plurality of second detection positions arranged on the rack, and a second LED lamp, a second photoelectric sensor and a second optical filter which are correspondingly arranged on two sides of each second detection position, wherein the second LED lamp and the second optical filter generate a second wavelength.
8. The hemagglutination analyzer of any one of claims 5-7, wherein the detection assembly comprises: and a third transmission optical detection component which is arranged on the frame and performs immunoturbidimetry detection.
9. The hemagglutination analyzer of any one of claims 5-7, wherein the detection assembly comprises: and a fourth transmission optical detection member disposed on the frame for detecting by a chromogenic substrate method.
10. The hemagglutination analyzer of any one of claims 5-7, wherein the first transmissive optical member, the second transmissive optical member, in part or in whole, can be multiplexed for immunoturbidimetric or chromogenic substrate assay.
11. The coagulometer according to any of claims 5-7, characterized in that said mobile filling assembly is used for both reagent filling and blood filling. The method comprises the following steps: the double-purpose filling device comprises a first three-dimensional moving part arranged on the rack, a first suction control part connected with the first three-dimensional moving part, and a double-purpose filling needle connected with the first suction control part.
12. The hemagglutination analyzer of any one of claims 5-7, wherein the mobile priming assembly comprises a reagent priming member and a blood sample priming member;
the reagent filling member includes: a second three-dimensional moving member disposed on the frame, a second aspiration control member connected to the second three-dimensional moving member, and a reagent filling needle connected to the second aspiration control member;
the blood sample filling member comprises: the blood sample feeding device comprises a third three-dimensional moving member arranged on the rack, a third suction control member connected with the third three-dimensional moving member, and a blood sample feeding needle connected with the third suction control member.
13. The hemagglutination analyzer of any one of claims 5-7, wherein the detection assembly further comprises: and the magnetic bead method detection channel is arranged on the rack.
14. The hemagglutination analyzer of any one of claims 5-7, wherein the movable grip has a homogenization function, the movable grip comprising: the reaction cup blending mechanism comprises a fourth three-dimensional moving member arranged on the rack, a hand grip structure connected with the fourth three-dimensional moving member, and a reaction cup blending structure arranged on the hand grip structure.
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