CN114895045A - Full-automatic biochemical and immune integrated detection equipment - Google Patents
Full-automatic biochemical and immune integrated detection equipment Download PDFInfo
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- CN114895045A CN114895045A CN202210470872.9A CN202210470872A CN114895045A CN 114895045 A CN114895045 A CN 114895045A CN 202210470872 A CN202210470872 A CN 202210470872A CN 114895045 A CN114895045 A CN 114895045A
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- 238000001514 detection method Methods 0.000 title claims abstract description 108
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 365
- 239000000523 sample Substances 0.000 claims description 101
- 230000007246 mechanism Effects 0.000 claims description 55
- 239000002699 waste material Substances 0.000 claims description 39
- 230000003287 optical effect Effects 0.000 claims description 37
- 238000010438 heat treatment Methods 0.000 claims description 22
- 238000006243 chemical reaction Methods 0.000 claims description 21
- 238000005842 biochemical reaction Methods 0.000 claims description 12
- 238000003018 immunoassay Methods 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 11
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- 239000007788 liquid Substances 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 238000000504 luminescence detection Methods 0.000 description 6
- 238000004020 luminiscence type Methods 0.000 description 6
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- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
Abstract
The invention relates to a full-automatic biochemical and immune integrated detection device, which comprises a shell, a liquid-moving device, a biochemical detection device, a light-emitting detection device, a reagent turntable device, a reagent channel device, a reagent gripping device and a sample frame device, wherein the device integrates biochemical detection and light-emitting detection functions, can perform large-batch reagent detection, improves the detection efficiency of the detection device, improves the detection capacity, and is more suitable for large-batch detection occasions; meanwhile, the reagent channel device in the equipment greatly improves the containing capacity of the reagent units in the equipment, and improves the batch detection efficiency.
Description
Technical Field
The invention belongs to the technical field of in-vitro diagnosis and detection, and relates to full-automatic biochemical and immune integrated detection equipment.
Background
The chemiluminescence immunoassay is an analyzer which is mainly used for measuring the components and the content of substances such as antigen and antibody contained in a sample by detecting the luminous intensity of a luminous reaction liquid corresponding to the sample to be measured. The biochemical analyzer is mainly an analyzer which mixes a sample to be tested and a corresponding reagent in a reaction cup, tests the colorimetric value of the mixed solution through chemical reaction, and determines indexes of various biochemical analysis items through calculation and analysis. At present, immunological detection and biochemical detection are carried out by 2 instruments separately. The detection mechanism needs to buy two instruments, namely a biochemical analyzer and an immunoassay, separately to realize biochemical and immunological detection. For primary hospitals, the simultaneous purchase of two instruments will certainly have more requirements on site and purchase cost
If the testee needs to carry out immune and biochemical detection at the same time, more than 2 tubes of serum need to be collected, or the manual sample separation is carried out to obtain at least two parts of serum, which are respectively detected on an immune instrument and a biochemical instrument. Or the same sample is detected on an immunization instrument and a biochemical instrument in sequence. Therefore, the pain of the blood collected by the person to be detected is increased, the working efficiency is reduced, and the time of detection report is delayed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention discloses a full-automatic biochemical and immune integrated detection device which integrates biochemical detection and luminescence detection functions, can perform large-batch reagent detection, improves the detection efficiency of the detection device, improves the detection capacity, and is more suitable for large-batch detection occasions.
The invention particularly discloses full-automatic biochemical and immune integrated detection equipment which is characterized by comprising a shell, a liquid-transferring device, a biochemical detection device, a light-emitting detection device, a reagent turntable device, a reagent channel device, a reagent grabbing device and a sample rack device,
the reagent turntable device comprises a reagent heating component and a bearing rotary component, wherein the reagent heating component is arranged below the bearing rotary component, and the reagent heating component and the bearing rotary component adopt a coaxial nested structure; the supporting rotary component is connected with a plurality of biochemical reagent rack units and luminous reagent rack units;
the biochemical reagent rack unit comprises a biochemical reagent rack clamping groove and a plurality of biochemical reagent strips, wherein each biochemical reagent strip is provided with a biochemical reagent hole, a biochemical reaction hole and a first gun head placing hole, and the bottom of the biochemical reaction hole is in a transparent state; the biochemical reagent rack clamping groove is provided with a first groove for accommodating biochemical reagent strips, the side wall of the first groove is provided with a first guide protrusion for matching with the biochemical reagent strips, the side wall of the biochemical reagent strips is provided with a first guide groove for matching with the first guide protrusion, and the biochemical reagent strips are pushed into the first groove of the biochemical reagent rack clamping groove and fixed through the matching of the first guide protrusion and the first guide groove;
the luminous reagent rack unit comprises a luminous reagent rack clamping groove and a plurality of luminous reagent strips, and each luminous reagent strip is provided with a luminous reagent hole, a luminous reaction hole and a second gun head placing hole; a second groove for accommodating the luminous reagent strip is formed in the luminous reagent rack clamping groove, a second guide protrusion for matching with the luminous reagent strip is arranged on the side wall of the second groove, a second guide groove for matching with the second guide protrusion is formed in the position of the side wall of the luminous reagent strip, and the luminous reagent strip is pushed into the second groove of the luminous reagent rack clamping groove and fixed through the matching of the second guide protrusion and the second guide groove;
the biochemical detection device is arranged on two sides of the reagent rotating disc device and comprises a light source shell, a light source probe, a first light-emitting source, a first optical sensor and a receiving probe, wherein the first light-emitting source is arranged in the light source shell, the light source probe receives detection light generated by the first light-emitting source, and the light source probe is arranged right above the biochemical reagent rack unit; the first optical sensor receives detection light from a receiving probe, and the receiving probe is arranged below the biochemical reagent rack unit; the light paths of the light source probe and the receiving probe in the vertical direction are kept collinear, the first light emitting source emits detection light which is transmitted to the light source probe and emitted out, the light source probe irradiates the detection light into a biochemical reaction hole of a biochemical reagent strip in a biochemical reagent rack unit, the detection light is transmitted to the receiving probe through the bottom of the biochemical reaction hole and is transmitted to the first light sensor through the receiving probe, and the first light sensor collects detection light signals and outputs electric signals;
the light-emitting detection device comprises a detection shell, a light path probe, a dichroic mirror, a second light-emitting source and a second light sensor, wherein the second light-emitting source, the dichroic mirror and the second light sensor are integrated in the detection shell, a light path connecting port is arranged on the detection shell and connected with the light path probe, the light path probe is positioned right above a light-emitting reagent rack unit and is coaxial with a light-emitting reaction hole of a light-emitting reagent strip, the dichroic mirror is arranged at an included angle and is positioned at the middle position of the second light-emitting source and the light path connecting port, the second light-emitting source emits laser which penetrates through the dichroic mirror and reaches the light path connecting port and is transmitted to the position of the light path probe by the light path connecting port, the laser irradiates into the light-emitting reaction hole of the light-emitting reagent strip in the light-emitting reagent rack unit, and a specific light signal generated by the light-emitting reaction hole reaches the light path connecting port through the light path probe, the specific optical signal is reflected by the dichroic mirror and transmitted to the second optical sensor, and the second optical sensor collects the specific optical signal and outputs a corresponding electrical signal.
Further, the bearing and rotation assembly comprises a central wheel, a driving shaft, a belt pulley, a bearing fixing seat and a bearing support frame, wherein the driving shaft is positioned below the central wheel, one end of the driving shaft is connected with the axis position of the central wheel, the other end of the driving shaft is downwards connected with the bearing fixing seat, the bearing fixing seat is arranged at the bottom of the shell, the bearing support frame is arranged below the central wheel and fixedly connected with the bottom of the shell, the driving shaft penetrates through the bearing support frame, and the belt pulley is coaxially arranged on the driving shaft between the bearing support frame and the bearing fixing seat; the center wheel is cross-shaped, four connecting arms extend outwards along the radial direction of the center wheel, fixing pieces used for supporting the biochemical reagent frame unit or the luminous reagent frame unit are arranged below each connecting arm, gaps are formed between every two adjacent fixing pieces in the circumferential direction of the center wheel, and the biochemical reagent frame unit or the luminous reagent frame unit is placed in the gaps.
Furthermore, the reagent heating component comprises a bearing bottom plate and a bottom plate supporting frame, the bearing bottom plate is fixed at the bottom of the shell through the bottom plate supporting frame, a through hole for accommodating the driving shaft is formed in the middle of the bearing bottom plate, an extending side wall is upwards arranged at the edge position of the through hole in the bearing bottom plate, an annular groove is fixedly connected above the extending side wall, the annular groove, the extending side wall and the bearing bottom plate jointly form an accommodating space, the accommodating space is used for arranging a sensor component for detecting temperature and a first heating body, and the first heating body is attached to one side of the extending side wall; the support bottom plate is provided with a through hole for passing through the detection light, and the through hole is coaxial with a light source probe of the biochemical detection device.
Further, the pipetting device comprises an X-direction pipetting assembly, a Y-direction pipetting assembly, a Z-direction pipetting assembly and a pipetting gun assembly, wherein the X-direction pipetting assembly, the Y-direction pipetting assembly and the Z-direction pipetting assembly are arranged at upper positions in the shell, and the X-direction pipetting assembly, the Y-direction pipetting assembly and the Z-direction pipetting assembly are combined to move to control the pipetting gun assembly to perform pipetting operation in the inner space of the shell;
the X-direction pipetting assembly comprises two groups of X-direction linear guide rails, the two groups of X-direction linear guide rails are parallel to each other, the two groups of X-direction linear guide rails are fixed at the left side and the right side positions in the shell respectively through vertical supporting vertical frames, each group of X-direction linear guide rails are respectively provided with an X-direction linear slider, one group of X-direction linear guide rails are also provided with an X-direction driving mechanism, and the X-direction driving mechanism is used for driving the X-direction linear sliders to move along the X-direction linear guide rails;
the Y-direction pipetting assembly comprises a Y-direction linear guide rail, the Y-direction linear guide rail is respectively vertical to the X-direction linear guide rails, two ends of the Y-direction linear guide rail are respectively fixedly connected to two groups of X-direction linear sliders of the X-direction pipetting assembly, a Y-direction linear slider and a Y-direction driving mechanism are arranged on the Y-direction linear guide rail, and the Y-direction linear slider can be driven to move along the Y-direction linear guide rail through the Y-direction driving mechanism;
the Z-direction liquid transfer mechanism comprises a Z-direction linear guide rail, the Z-direction linear guide rail is fixedly connected with a Y-direction linear slider, the Z-direction linear slider is respectively perpendicular to the X-direction linear guide rail and the Y-direction linear guide rail, a Z-direction linear slider and a Z-direction driving mechanism are arranged on the Z-direction linear guide rail, and the Z-direction driving mechanism drives the Z-direction linear slider to move along the Z-direction linear guide rail;
the pipetting gun assembly is fixed on the Z-direction linear slide block.
Furthermore, the reagent channel device comprises a reagent feeding channel and a reagent waste channel, the reagent feeding channel is fixedly connected with the reagent waste channel, a channel support is connected to the lower side of the reagent waste channel, and the channel support is fixed to the bottom of the shell.
Furthermore, the reagent feeding channel comprises a feeding channel shell, a first accommodating cavity for accommodating an undetected biochemical reagent rack unit or luminescent reagent rack unit is arranged in the feeding channel shell, vertical guide grooves are respectively arranged on the rear side wall, the left side wall and the right side wall of the first accommodating cavity, the biochemical reagent rack unit or the luminescent reagent rack unit is respectively matched with the vertical guide grooves, the biochemical reagent rack unit or the luminescent reagent rack unit is fixed in the horizontal direction through the vertical guide grooves on the three side walls, and the biochemical reagent rack unit or the luminescent reagent rack unit slides along the length direction of the vertical guide grooves; a second heating body is attached to the front side wall of the first accommodating cavity and used for keeping the temperature inside the reagent feeding channel stable; in addition, a temperature sensor is arranged on the inner side of the reagent feeding channel and used for monitoring the temperature change of the reagent feeding channel; a feeding limiting mechanism is arranged at the lower part of the vertical guide groove of the rear side wall in the first accommodating cavity and comprises a first limiting block, a second limiting groove, a reset spring and a hinge shaft, the first limiting block is connected with the second limiting groove through the hinge shaft, and the reset spring is arranged between the first limiting block and the second limiting groove; when the first limiting block is pressed into the second limiting groove, the reagent rack unit positioned in the first accommodating cavity falls down from the lower port of the first accommodating cavity; when the first limiting block is ejected by the return spring, the biochemical reagent rack unit or the luminous reagent rack unit in the first accommodating cavity is blocked by the first limiting block, and the biochemical reagent rack unit or the luminous reagent rack unit is kept in the first accommodating cavity.
Furthermore, the reagent waste channel comprises a waste channel shell, a second accommodating cavity for accommodating a biochemical reagent rack unit or a luminescent reagent rack unit which is detected is arranged in the waste channel shell, two limiting supporting bodies are arranged in the second accommodating cavity, the limiting supporting bodies are arranged at the corners of the second accommodating cavity, a waste limiting structure is arranged at the lower part of each limiting supporting body, the waste limiting structure comprises a second limiting body, the second limiting body is hinged in a second limiting groove of the limiting supporting body, and a reset spring is arranged between the second limiting body and the second limiting groove; when the biochemical reagent rack unit or the luminous reagent rack unit which is detected completely enters from the lower part of the reagent waste channel, the biochemical reagent rack unit or the luminous reagent rack unit can extrude the second limiting block to enable the second limiting block to press towards the second limiting groove, and when the biochemical reagent rack unit or the luminous reagent rack unit completely enters into the reagent waste channel, the second limiting block is popped up under the action of the reset spring, so that the biochemical reagent rack unit or the luminous reagent rack unit of the reagent waste channel can be limited from falling.
Furthermore, vertical guide blocks are respectively arranged on the left side, the right side and the rear side of the reagent rack clamping groove and are matched with the vertical guide groove of the feeding channel shell; the reagent frame clamping groove is characterized in that a first magnetic suction part and a positioning hole are formed in the bottom of the reagent frame clamping groove, and a limiting groove is further formed in the vertical guide block on the rear side of the reagent frame clamping groove.
Further, the reagent gripping device comprises an X-direction moving mechanism, a Z-direction moving mechanism and a moving arm;
the X-direction moving mechanism comprises an X-direction moving guide rail, an X-direction moving slide block and an X-direction power mechanism, the X-direction moving guide rail is fixed at the bottom of the shell, the X-direction moving slide block is connected with the X-direction moving guide rail in a sliding manner, and the X-direction power mechanism drives the X-direction moving slide block to move along the X-direction moving guide rail;
the Z-direction moving mechanism is fixed on the X-direction moving slide block, the Z-direction moving mechanism comprises a Z-direction moving guide rail, a Z-direction moving slide block and a Z-direction power mechanism, the Z-direction moving guide rail is fixed on the side of the X-direction moving slide block, the Z-direction moving slide block is connected with the Z-direction moving guide rail in a sliding manner, and the Z-direction power mechanism drives the Z-direction moving slide block to displace along the Z-direction moving guide rail;
the movable arm is connected to the Z-direction movable sliding block, and the tail end of the movable arm is provided with a limiting block matched with a limiting groove in the reagent rack clamping groove, a positioning screw matched with a positioning hole in the reagent rack clamping groove and a second magnetic suction part matched with the first magnetic suction part in the reagent rack clamping groove.
Furthermore, the sample rack device comprises a plurality of rows of sample tube fixing frames, each row of sample tube fixing frames comprises a fixing base and a test tube clamping block, and the fixing bases and the bottom of the shell are fixed by adopting a push-pull type limiting structure; the test tube clamp splice is provided with a plurality of sample tube clamp grooves, and each sample tube clamp groove is used for fixing a sample tube.
1) The biochemical and luminous integrated machine has the advantages that the biochemical detection device and the luminous detection device are arranged at the positions of the reagent rotating discs together, the corresponding reagent rotating disc devices are compatible with the biochemical reagent frame units and the luminous reagent frame units, and the biochemical and luminous detection functions can be realized in one instrument by matching different reagent frame units with the corresponding detection devices, and the biochemical and luminous detection functions are independent to each other and are efficient and accurate in detection; the device integrates two detection functions, reduces the occupied area under the condition of ensuring the complete detection functions, has small volume and convenient operation, and greatly facilitates operators.
2) The reagent channel device comprises a reagent feeding channel and a reagent waste channel, wherein a reagent rack unit in the reagent feeding channel slides from the upper end into the reagent feeding channel to be prestored; and to the reagent frame unit that detects the completion, snatch automatically by reagent grabbing device and transfer to and store in the reagent waste material passageway, can once only take out discarded reagent frame unit after detecting the completion at last. Can smoothly carry out reagent frame unit replacement, degree of automation height through reagent passage device and reagent grabbing device cooperation in this device, and reagent passage device has promoted the splendid attire capacity of reagent unit by a wide margin, has realized the full-automatic high flux detection of single one-person reagent strip.
3) According to the invention, the reagent strip adopts single-person, modularized and integrated design, the pipetting gun heads, the reagent holes and the reaction holes are all integrated in the single-person reagent strip, single-person item detection can be carried out in the detection process, detection items can be flexibly adjusted according to needs, various reagent strip tray frames and reagent strips thereof are placed, a plurality of detection items are simultaneously carried out in a single system, the number of the pipetting gun heads, the reagent holes and the reaction holes on the reagent strip is adjusted according to the needs of the detection items, the detection mode is flexible and changeable, and the practicability and the universality are strong; and through adopting modular reagent strip structure, need not to increase the liquid way structure inside the instrument, can realize single project detection through the reagent strip of liquid-transfering device cooperation individual person, reduced the fault rate and the cost of instrument.
4) The luminescence detection device of the invention adopts homogeneous luminescence detection principle to carry out immunoassay, and does not need to be separated and cleaned in the detection process; therefore, a separation cleaning device is not required to be arranged in the equipment, the whole detection process is simple and quick, and the detection efficiency is further improved.
Drawings
FIG. 1 is a schematic view of the overall structure of the fully automatic biochemical and immunological integrated detection apparatus in this embodiment;
FIG. 2a is a schematic view of the mounting of the light source probe in the biochemical detecting device according to the embodiment;
FIG. 2b is a schematic structural diagram of the biochemical detection device according to the embodiment;
FIG. 2c is a schematic view illustrating an installation of an optical path probe in the light-emitting detection apparatus of the present embodiment;
FIG. 2d is a schematic structural diagram of the light-emitting detection device in this embodiment
FIG. 3 is a schematic perspective view of the pipetting device of this embodiment;
FIG. 4 is a schematic view showing the mounting of the pipette gun assembly in the pipetting device of this embodiment;
FIG. 5 is a schematic structural view of a reagent tray apparatus according to this embodiment;
FIG. 6 is a schematic view showing the position of a reagent rack unit in the reagent tray device according to this embodiment;
FIG. 7 is a schematic top view of the reagent tray apparatus of this embodiment;
FIG. 8 is a side cross-sectional view of the location A-A in FIG. 7;
FIG. 9a is a schematic view of a clamping groove of the biochemical reagent rack in this embodiment;
FIG. 9b is a schematic view of a clamping groove of the luminescent reagent rack in this embodiment;
FIG. 10 is a schematic perspective view of the reagent passage device in this embodiment;
FIG. 11 is a schematic view showing the internal structure of the reagent passage device in this embodiment;
FIG. 12 is a schematic top view of the reagent passage device of this embodiment;
FIG. 13 is a schematic perspective view of the reagent grasping apparatus according to this embodiment;
FIG. 14 is a schematic view showing the structure of a moving arm in the reagent holding device according to this embodiment;
fig. 15 is a schematic view of the mounting structure of the sample rack device in this embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1:
referring to fig. 1, this embodiment 1 specifically discloses a full-automatic biochemical and immune integrated detection apparatus, which includes a housing 1, a pipetting device 2, a detection device 7, a reagent rotating disk device 3, a reagent channel device 5, a reagent grasping device 6, and a sample rack device 4. Wherein the detection means 7 comprises biochemical detection means and luminescence detection means.
As shown in fig. 5 to 8, the reagent turntable device 3 includes a reagent heating assembly 302 and a supporting rotary assembly 301, the reagent heating assembly 302 is disposed below the supporting rotary assembly 301, and the reagent heating assembly 302 and the supporting rotary assembly 301 are in a coaxial nested structure.
The supporting and rotating assembly 301 comprises a central wheel 3011, a driving shaft 3012, a belt pulley 3013, a bearing fixing seat 3014 and a bearing support seat 3015, wherein the driving shaft 3012 is located below the central wheel 3011, one end of the driving shaft 3012 is connected to the axis position of the central wheel 3011, the other end is connected to the bearing fixing seat 3014 downward, the bearing fixing seat 3014 is installed at the bottom of the housing 1, the bearing support seat 3015 is installed below the central wheel 3011, the bearing support seat 3015 is fixedly connected to the bottom of the housing 1, the driving shaft 3012 penetrates through the bearing support seat 3015, and the belt pulley 3013 is coaxially installed on the driving shaft 3012 between the bearing support seat 3015 and the bearing fixing seat 3014; in this embodiment, the central wheel 3011 is cross-shaped, and the central wheel 3011 extends radially outward to form four connecting arms, a fixing component 3016 for supporting the biochemical reagent rack unit 303a or the luminescent reagent rack unit 303b is disposed below each connecting arm, and a gap is disposed between adjacent fixing components 3016 along the circumferential direction of the central wheel 3011, and the biochemical reagent rack unit 303a or the luminescent reagent rack unit 303b is disposed in the gap; since different types of reagent strips are fixed by the reagent strip clamping grooves with the same size in this embodiment, the fixing positions of the supporting rotary component 301 for the biochemical reagent rack unit and the luminescent reagent rack unit in this embodiment in the market can be compatible with each other.
The reagent heating assembly 302 comprises a bearing bottom plate 3021 and a bottom plate support 3022, the bearing bottom plate 3021 is fixed to the bottom of the housing 1 through the bottom plate support 3022, a through hole for accommodating the driving shaft 3012 is formed in the middle of the bearing bottom plate 3021, an extending side wall is upward formed in the edge position of the through hole in the bearing bottom plate 3021, an annular block 3023 is fixedly connected above the extending side wall, the annular block 3023, the extending side wall and the bearing bottom plate 3021 together form an accommodating space, the accommodating space is used for setting a sensor assembly 3025 for detecting temperature and a first heating body 3024, and the first heating body 3024 is attached to one side of the extending side wall; in order to facilitate the passage of the detection laser, a through hole is formed in the supporting base plate 3021, and the through hole is coaxial with the light source probe 702 of the biochemical detection device.
In this embodiment, the biochemical detection device is disposed on both sides of the reagent turntable device 3, as shown in fig. 2a and 2b, the biochemical detection device includes a light source housing, a light source probe 702, a first light emitting source 701a, a first light sensor 705a, and a receiving probe 704, the first light emitting source 701 is disposed inside the light source housing, the light source probe 702 receives probe light generated by the first light emitting source 701a, and the light source probe 702 is disposed right above the biochemical reagent rack unit 303 a; the first optical sensor 705a receives the probe light from the receiving probe 704, and the receiving probe 704 is disposed below the biochemical reagent rack unit 303 a; the light paths of the light source probe 702 and the receiving probe 704 in the vertical direction are kept collinear, the first light emitting source 701a emits detection light, the detection light is transmitted to the light source probe 702 and emitted out, the light source probe 702 irradiates the detection light into a biochemical reaction hole of a biochemical reagent strip 304a in the biochemical reagent rack unit 303a, the detection light is transmitted to the receiving probe 704 through the bottom of the biochemical reaction hole and is transmitted to the first light sensor 705a through the receiving probe 704, and the first light sensor 705a collects detection light signals and outputs electric signals.
In this embodiment, the light source probe 702 and the first light emitting source 701a are integrated inside a light source housing, the light source housing is disposed on the upper side of the reagent turntable device 3, the first optical sensor 705a and the receiving probe 704 are disposed on the lower side of the reagent turntable device 3, and the light source housing and the first optical sensor 705a are relatively fixed in position by a connecting arm, and the connecting arm is fixedly connected inside the housing 1; the light source probe 702 and the receiving probe 704 are collinear in the vertical direction.
In addition, as an alternative, in the biochemical detection apparatus of this embodiment, the first optical fiber 703 may be disposed between the light source probe 702 and the first light source 701a, since the light source probe 702 and the first light source 701a transmit light signals through the first optical fiber 703, and the first optical sensor 705a and the receiving probe 704 transmit light signals through the second optical fiber 706, it is only necessary to keep the light paths of the light source probe 702 and the receiving probe 704 collinear, and the first light source 701a and the first optical sensor 705a may be fixed inside the housing 1, and the fixing positions are not limited, so as to avoid interference of the heat of the light source on the reaction of the reagent.
Correspondingly, as shown in fig. 6, the biochemical reagent rack unit includes a biochemical reagent rack clamping groove 303a and a plurality of biochemical reagent strips 304a, each biochemical reagent strip 304a is provided with a biochemical reagent hole, a biochemical reaction hole and a first gun head placing hole, wherein the bottom of the biochemical reaction hole is transparent so as to facilitate transmission of detection light; as shown in fig. 9a, a first groove 3031a for accommodating the biochemical reagent strip 304a is arranged on the biochemical reagent holder clamping groove 303, a first guide protrusion 3032a for fitting the biochemical reagent strip 304a is arranged on a side wall of the first groove 3031a, a first guide groove for fitting the first guide protrusion is arranged on a side wall of the biochemical reagent strip 304a, and the biochemical reagent strip 304a is pushed into the first groove 3031a of the biochemical reagent holder clamping groove 303a and fixed by the fit of the first guide protrusion 3032a and the first guide groove; the biochemical reagent holder clamping groove 303a is provided with vertical guide blocks 3033a at the left side, the right side and the rear side thereof, respectively, and the vertical guide blocks 3033a are matched with the vertical guide grooves 5013 of the feed channel housing. Further, a first magnetic suction part and a positioning hole 3034a are arranged at the bottom of the biochemical reagent holder clamping groove 303a, and a limiting groove is further arranged on the vertical guide block at the rear side of the biochemical reagent holder clamping groove 303 a.
The luminescence detection device in this embodiment is disposed on one side of the reagent turntable device 3, and as shown in fig. 2c and fig. 2d, is specifically disposed above the reagent turntable device 3, the luminescence detection device comprises a detection shell 711, an optical path probe 710, a dichroic mirror 707, a second luminous source 701b and a second light sensor 705b, the second light emitting source 701b, the dichroic mirror 707 and the second light sensor 705b are integrated inside the detection housing 711, the detection shell 711 is provided with an optical path connection port 708, the optical path connection port 708 is connected with an optical path probe 710, the optical path probe 710 is positioned right above the luminescent reagent rack unit, the light path probe 710 is coaxial with the luminescence reaction hole of the luminescence reagent strip, the dichroic mirror 707 is arranged with an included angle, for example, the dichroic mirror 707 in this embodiment is arranged at 45 °, and is aimed to transmit the reflected specific light signal to the second light sensor 705 b; the dichroic mirror 707 is located in the middle of the second light source 701b and the optical path connection port 708, the second light source 701b emits laser, which penetrates through the dichroic mirror 707 and reaches the optical path connection port 708, and is transmitted to the optical path probe 710 through the optical path connection port 708, the laser is irradiated into the luminescence reaction hole of the luminescence reagent strip in the luminescence reagent rack unit, a specific light signal generated by the luminescence reaction hole is reflected by the dichroic mirror 707 and transmitted to the second light sensor 705b, and the second light sensor 705b collects the specific light signal and outputs a corresponding electrical signal.
In this embodiment, the optical path probe 710 can be integrated inside the detection housing 711, so the light emitting detection device in this embodiment can be integrally disposed above the reagent rotating disk device 3, but it should be noted that, as an alternative, in the present invention, a third optical fiber 709 can be disposed between the optical path connection port 708 and the optical path probe 710, and an optical signal is transmitted between the optical path connection port 708 and the optical path probe 710 through the third optical fiber 709, in the present invention, the optical path probe 710 and the optical path connection port 708 of the detection housing 711 adopt a flexible third optical fiber 709 for optical signal transmission, so the optical path probe 710 can be held above the reaction hole of the light emitting reagent strip, and the fixing position of other structures of the light emitting detection device is not limited.
Correspondingly, as shown in fig. 6, the luminescent reagent rack unit includes a luminescent reagent rack clamping groove 303b and a plurality of luminescent reagent strips 304b, and each luminescent reagent strip 304b is provided with a luminescent reagent hole, a luminescent reaction hole, and a second gun head placing hole; as shown in fig. 9b, a second groove 3031b for accommodating the luminescent reagent strip 304b is arranged on the luminescent reagent holder clamping groove 303b, a second guide protrusion 3032b for matching with the luminescent reagent strip 304b is arranged on a side wall of the second groove 3031b, a second guide groove for matching with the second guide protrusion is arranged on a side wall of the luminescent reagent strip 304b, and the luminescent reagent strip 304b is pushed into the second groove 3031b of the luminescent reagent holder clamping groove 303b and fixed by the matching of the second guide protrusion 3032b and the second guide groove; the left, right and rear sides of the reagent frame slot 303b are provided with vertical guides 3033b, respectively, and the vertical guides 3033b mate with the vertical guide slots 5013 of the feed channel housing. Further, a first magnetic suction part and a positioning hole 3034b are arranged at the bottom of the luminous reagent holder clamping groove 303b, and a limiting groove is further arranged on the vertical guide block at the rear side of the luminous reagent holder clamping groove 303 b.
Referring to fig. 3 and 4, the pipetting device 2 includes an X-direction pipetting unit, a Y-direction pipetting unit, a Z-direction pipetting unit, and a pipetting gun unit 210, the X-direction pipetting unit, the Y-direction pipetting unit, and the Z-direction pipetting unit are disposed at upper positions inside the housing 1, and the combined movement of the X-direction pipetting unit, the Y-direction pipetting unit, and the Z-direction pipetting unit controls the pipetting gun unit 210 to perform pipetting operation in the space inside the housing.
Specifically, the X-direction pipetting assembly comprises two groups of X-direction linear guide rails 201, the two groups of X-direction linear guide rails 201 are parallel to each other, the two groups of X-direction linear guide rails 201 are fixed at left and right positions in the housing 1 through vertical support stands 211, each group of X-direction linear guide rails 201 is provided with an X-direction linear slider 202, one group of X-direction linear guide rails 201 is further provided with an X-direction driving mechanism 203, and the X-direction driving mechanism 203 is used for driving the X-direction linear sliders 202 to move along the X-direction linear guide rails 201; the Y-direction pipetting assembly comprises a Y-direction linear guide rail 204, the Y-direction linear guide rail 204 is respectively vertical to the X-direction linear guide rail 201, two ends of the Y-direction linear guide rail 204 are respectively fixedly connected to two groups of X-direction linear sliders 202 of the X-direction pipetting assembly, a Y-direction linear slider 205 and a Y-direction driving mechanism 206 are arranged on the Y-direction linear guide rail 204, and the Y-direction linear slider 205 can be driven to move along the Y-direction linear guide rail 204 by the Y-direction driving mechanism 206; the Z-direction pipetting mechanism comprises a Z-direction linear guide rail 207, the Z-direction linear guide rail 207 is fixedly connected with a Y-direction linear slide block 205, the Z-direction linear guide rail 207 is respectively vertical to the X-direction linear guide rail 201 and the Y-direction linear guide rail 204, a Z-direction linear slide block 208 and a Z-direction driving mechanism 209 are arranged on the Z-direction linear guide rail 207, and the Z-direction driving mechanism 209 drives the Z-direction linear slide block 208 to move along the Z-direction linear guide rail 207; the pipetting gun assembly 210 is fixed on the Z-direction linear slide block 208, and the pipetting gun assembly 210 can move vertically and horizontally through the synchronous matching of the X, Y, Z-direction linear guide rail and the linear slide block, so that the pipetting operation can be performed in the three-dimensional space of the shell.
After a reagent rack unit on the reagent carousel device 3 is used, the reagent gripping device 6 moves to the lower side of the reagent rack unit, the reagent rack unit is attracted by magnetic force, the reagent rack unit is transversely pulled out of the reagent carousel device 3, and the pulled-out reagent rack unit is pushed to the reagent waste channel 502 for storage. The reagent gripping device 6 will then move to the reagent feed path 501 and magnetically pick up a new reagent rack unit, which is then transferred to the reagent rotor device 3 and pushed into the vacant position in the reagent rotor device 3.
The reagent channel device 5 comprises a reagent feeding channel 501 and a reagent waste channel 502, as shown in fig. 10-12, the reagent feeding channel 501 and the reagent waste channel 502 are fixedly connected, a channel bracket 503 is connected to the lower side of the reagent waste channel 502, and the channel bracket 503 is fixed to the bottom of the housing 1.
The reagent feeding channel 501 comprises a feeding channel shell, a first accommodating cavity 5011 for placing an undetected biochemical reagent rack unit or luminous reagent rack unit is arranged in the feeding channel shell, vertical guide grooves 5013 are respectively arranged on the rear side wall, the left side wall and the right side wall of the first accommodating cavity 5011, the reagent rack unit is matched with the vertical guide grooves 5013, the biochemical reagent rack unit or the luminous reagent rack unit is fixed in the horizontal direction through the vertical guide grooves 5013 on the three side walls, and meanwhile, the biochemical reagent rack unit or the luminous reagent rack unit respectively slides along the length direction of the vertical guide grooves 5013; a second heating body 5012 is attached to the front side wall of the first accommodating cavity 5011, and the second heating body 5012 is used for keeping the temperature inside the reagent feeding channel 501 stable; also provided inside the reagent feeding channel 501 is a temperature sensor 5014, which temperature sensor 5014 monitors the temperature change of the reagent feeding channel 501. Furthermore, the lower portion of the vertical guide groove 5013 of the rear side wall of the first accommodating cavity 5011 is provided with a feeding limiting mechanism, specifically, the feeding limiting mechanism comprises a first limiting block, a second limiting groove, a return spring and a hinge shaft, the first limiting block is connected with the second limiting groove through the hinge shaft, and the return spring is arranged between the first limiting block and the second limiting groove. When the first stopper is pressed into the second stopper groove, the reagent rack units of different types located in the first housing cavity 5011 may fall from the lower port of the first housing cavity 5011; when the first stopper is ejected by the return spring, the different types of reagent rack units located in the first accommodation cavity 5011 are blocked by the first stopper, and the corresponding reagent rack units remain inside the first accommodation cavity 5011.
The reagent waste channel 502 comprises a waste channel shell, a second accommodating cavity 5021 for placing a detected biochemical reagent rack unit or luminescent reagent rack unit is arranged inside the waste channel shell, two limiting supports 5022 are arranged inside the second accommodating cavity 5021, the limiting supports 5022 are arranged at the corners of the second accommodating cavity 5021, a waste limiting structure is arranged at the lower part of each limiting support 5022, the waste limiting structure comprises a second limiting body, the second limiting body is hinged in a second limiting groove of the limiting support 5022, and a reset spring is arranged between the second limiting body and the second limiting groove; when biochemical reagent frame unit or luminous reagent frame unit that finish detecting enters by reagent waste passageway 502 below, the reagent frame unit that corresponds can extrude the second stopper, makes the second stopper press to the second spacing inslot, and inside biochemical reagent frame unit or luminous reagent frame unit got into reagent waste passageway 502 completely, the second stopper popped out under reset spring effect, can restrict reagent waste passageway 502's different grade type reagent frame unit and fall.
Furthermore, in order to count the biochemical reagent rack units or the luminescent reagent rack units of the reagent feeding channel 501 and the reagent waste channel 502, respectively, the lower parts of the reagent feeding channel 501 and the reagent waste channel 502 are respectively provided with a counting sensor, and when the biochemical reagent rack units or the luminescent reagent rack units enter or fall down, the counting sensors respectively output corresponding counting signals.
As shown in fig. 13 and 14, the reagent grasping apparatus 6 includes an X-direction moving mechanism, a Z-direction moving mechanism, and a moving arm 607, the X-direction moving mechanism includes an X-direction moving guide 603, an X-direction moving slider 602, and an X-direction power mechanism 601, the X-direction moving guide 603 is fixed to the bottom of the housing 1, the X-direction moving slider 602 is slidably connected to the X-direction moving guide 603, and the X-direction power mechanism 601 drives the X-direction moving slider 602 to displace along the X-direction moving guide 603; the Z-direction moving mechanism is fixed on the X-direction moving slider 602, wherein the Z-direction moving mechanism comprises a Z-direction moving guide rail 606, a Z-direction moving slider 605 and a Z-direction power mechanism 604, the Z-direction moving guide rail 606 is fixed on the side of the X-direction moving slider 602, the Z-direction moving slider 605 is connected to the Z-direction moving guide rail 606 in a sliding manner, and the Z-direction power mechanism 604 drives the Z-direction moving slider 605 to displace along the Z-direction moving guide rail 606; the moving arm 607 is connected to the Z-direction moving slider 605, and the end of the moving arm 607 is provided with a limit block 608 matching with a limit slot on the reagent rack clamp slot 303, a positioning screw 610 matching with a limit hole on the reagent rack clamp slot 303, and a second magnetic part 609 matching with a first magnetic part on the reagent rack clamp slot 303. The moving arm 607 is controlled to move back and forth in the housing by the cooperation of the X-direction moving mechanism and the Z-direction moving mechanism, thereby transferring the reagent feeding path 501 and the reagent waste path 502 in the reagent path unit 5 and the different types of reagent rack units in the reagent rotor apparatus 3.
More specifically, as shown in fig. 15, the sample rack device 4 includes a plurality of rows of test tube holders, each row of test tube holders includes a fixing base 401 and a test tube clamping block 402, the fixing base 401 and the bottom of the housing 1 are fixed by a push-pull type limiting structure, for example, in this embodiment, a guide groove is disposed on a side of the fixing base 401, a guide strip is disposed on the corresponding bottom of the housing 1, and the fixing base can move only along the Y-axis direction, i.e., the left-right direction of the housing, by matching the guide strip and the guide groove; the test tube clamping block is provided with a plurality of test tube clamping grooves 403, and each test tube clamping groove 403 is used for fixing a sample tube 404.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (10)
1. A full-automatic biochemical and immune integrated detection device is characterized by comprising a shell, a liquid-transfering device, a biochemical detection device, a luminous detection device, a reagent turntable device, a reagent channel device, a reagent gripping device and a sample rack device,
the reagent turntable device comprises a reagent heating component and a bearing rotary component, wherein the reagent heating component is arranged below the bearing rotary component, and the reagent heating component and the bearing rotary component adopt a coaxial nested structure; the supporting rotary component is connected with a plurality of biochemical reagent rack units and luminous reagent rack units;
the biochemical reagent rack unit comprises a biochemical reagent rack clamping groove and a plurality of biochemical reagent strips, wherein each biochemical reagent strip is provided with a biochemical reagent hole, a biochemical reaction hole and a first gun head placing hole, and the bottom of the biochemical reaction hole is in a transparent state; the biochemical reagent rack clamping groove is provided with a first groove for accommodating biochemical reagent strips, the side wall of the first groove is provided with a first guide protrusion for matching with the biochemical reagent strips, the side wall of the biochemical reagent strips is provided with a first guide groove for matching with the first guide protrusion, and the biochemical reagent strips are pushed into the first groove of the biochemical reagent rack clamping groove and fixed through the matching of the first guide protrusion and the first guide groove;
the luminous reagent rack unit comprises a luminous reagent rack clamping groove and a plurality of luminous reagent strips, and each luminous reagent strip is provided with a luminous reagent hole, a luminous reaction hole and a second gun head placing hole; a second groove for accommodating a luminous reagent strip is formed in the luminous reagent rack clamping groove, a second guide protrusion for matching with the luminous reagent strip is arranged on the side wall of the second groove, a second guide groove for matching with the second guide protrusion is formed in the side wall of the luminous reagent strip, and the luminous reagent strip is pushed into the second groove of the luminous reagent rack clamping groove and fixed through the matching of the second guide protrusion and the second guide groove;
the biochemical detection device is arranged on two sides of the reagent rotating disc device and comprises a light source shell, a light source probe, a first light emitting source, a first optical sensor and a receiving probe, wherein the first light emitting source is arranged in the light source shell, the light source probe receives detection light generated by the first light emitting source, and the light source probe is arranged right above the biochemical reagent rack unit; the first optical sensor receives detection light from a receiving probe, and the receiving probe is arranged below the biochemical reagent rack unit; the light paths of the light source probe and the receiving probe in the vertical direction are kept collinear, the first light emitting source emits detection light which is transmitted to the light source probe and emitted out, the light source probe irradiates the detection light into a biochemical reaction hole of a biochemical reagent strip in a biochemical reagent rack unit, the detection light is transmitted to the receiving probe through the bottom of the biochemical reaction hole and is transmitted to the first light sensor through the receiving probe, and the first light sensor collects detection light signals and outputs electric signals;
the light-emitting detection device comprises a detection shell, a light path probe, a dichroic mirror, a second light-emitting source and a second light sensor, wherein the second light-emitting source, the dichroic mirror and the second light sensor are integrated in the detection shell, a light path connecting port is arranged on the detection shell and connected with the light path probe, the light path probe is positioned right above a light-emitting reagent rack unit and is coaxial with a light-emitting reaction hole of a light-emitting reagent strip, the dichroic mirror is arranged at an included angle and is positioned at the middle position of the second light-emitting source and the light path connecting port, the second light-emitting source emits laser which penetrates through the dichroic mirror and reaches the light path connecting port and is transmitted to the position of the light path probe by the light path connecting port, the laser irradiates into the light-emitting reaction hole of the light-emitting reagent strip in the light-emitting reagent rack unit, and a specific light signal generated by the light-emitting reaction hole reaches the light path connecting port through the light path probe, the specific optical signal is reflected by the dichroic mirror and transmitted to the second optical sensor, and the second optical sensor collects the specific optical signal and outputs a corresponding electrical signal.
2. The integrated biochemical-immunoassay test device as set forth in claim 1, wherein: the bearing rotary assembly comprises a central wheel, a driving shaft, a belt pulley, a bearing fixing seat and a bearing supporting frame, wherein the driving shaft is positioned below the central wheel, one end of the driving shaft is connected with the axis position of the central wheel, the other end of the driving shaft is downwards connected with the bearing fixing seat, the bearing fixing seat is arranged at the bottom of the shell, the bearing supporting frame is arranged below the central wheel and fixedly connected with the bottom of the shell, the driving shaft penetrates through the bearing supporting frame, and the belt pulley is coaxially arranged on the driving shaft between the bearing supporting frame and the bearing fixing seat; the center wheel is cross-shaped, four connecting arms extend outwards along the radial direction of the center wheel, fixing pieces used for supporting the biochemical reagent frame unit or the luminous reagent frame unit are arranged below each connecting arm, gaps are formed between every two adjacent fixing pieces in the circumferential direction of the center wheel, and the biochemical reagent frame unit or the luminous reagent frame unit is placed in the gaps.
3. The integrated biochemical-immunoassay test device according to claim 2, wherein: the reagent heating assembly comprises a bearing bottom plate and a bottom plate supporting frame, the bearing bottom plate is fixed to the bottom of the shell through the bottom plate supporting frame, a through hole for accommodating a driving shaft is formed in the middle of the bearing bottom plate, an extending side wall is upwards arranged at the edge of the through hole in the bearing bottom plate, an annular groove is fixedly connected above the extending side wall, the annular groove, the extending side wall and the bearing bottom plate jointly form an accommodating space, the accommodating space is used for arranging a sensor assembly for detecting temperature and a first heating body, and the first heating body is attached to one side of the extending side wall; the support bottom plate is provided with a through hole for passing through the detection light, and the through hole is coaxial with a light source probe of the biochemical detection device.
4. The integrated biochemical-immunoassay test device as set forth in claim 1, wherein: the pipetting device comprises an X-direction pipetting assembly, a Y-direction pipetting assembly, a Z-direction pipetting assembly and a pipetting gun assembly, the X-direction pipetting assembly, the Y-direction pipetting assembly and the Z-direction pipetting assembly are arranged at upper positions in the shell, and the X-direction pipetting assembly, the Y-direction pipetting assembly and the Z-direction pipetting assembly are combined to move to control the pipetting gun assembly to perform pipetting operation in the inner space of the shell;
the X-direction pipetting assembly comprises two groups of X-direction linear guide rails, the two groups of X-direction linear guide rails are parallel to each other, the two groups of X-direction linear guide rails are fixed at the left side and the right side positions in the shell respectively through vertical supporting vertical frames, each group of X-direction linear guide rails are respectively provided with an X-direction linear slider, one group of X-direction linear guide rails are also provided with an X-direction driving mechanism, and the X-direction driving mechanism is used for driving the X-direction linear sliders to move along the X-direction linear guide rails;
the Y-direction pipetting assembly comprises a Y-direction linear guide rail, the Y-direction linear guide rail is respectively vertical to the X-direction linear guide rails, two ends of the Y-direction linear guide rail are respectively fixedly connected to two groups of X-direction linear sliders of the X-direction pipetting assembly, a Y-direction linear slider and a Y-direction driving mechanism are arranged on the Y-direction linear guide rail, and the Y-direction linear slider can be driven to move along the Y-direction linear guide rail through the Y-direction driving mechanism;
the Z-direction liquid transfer mechanism comprises a Z-direction linear guide rail, the Z-direction linear guide rail is fixedly connected with a Y-direction linear slider, the Z-direction linear slider is respectively perpendicular to the X-direction linear guide rail and the Y-direction linear guide rail, a Z-direction linear slider and a Z-direction driving mechanism are arranged on the Z-direction linear guide rail, and the Z-direction driving mechanism drives the Z-direction linear slider to move along the Z-direction linear guide rail;
the pipetting gun assembly is fixed on the Z-direction linear slide block.
5. The integrated biochemical-immunoassay test device as set forth in claim 1, wherein: the reagent channel device comprises a reagent feeding channel and a reagent waste channel, the reagent feeding channel is fixedly connected with the reagent waste channel, a channel support is connected to the lower side of the reagent waste channel, and the channel support is fixed to the bottom of the shell.
6. The integrated biochemical-immunoassay test device according to claim 5, wherein: the reagent feeding channel comprises a feeding channel shell, a first accommodating cavity for accommodating an undetected biochemical reagent rack unit or luminescent reagent rack unit is arranged in the feeding channel shell, vertical guide grooves are respectively formed in the rear side wall, the left side wall and the right side wall of the first accommodating cavity, the biochemical reagent rack unit or the luminescent reagent rack unit is respectively matched with the vertical guide grooves, the biochemical reagent rack unit or the luminescent reagent rack unit is fixed in the horizontal direction through the vertical guide grooves in the three side walls, and the biochemical reagent rack unit or the luminescent reagent rack unit slides along the length direction of the vertical guide grooves; a second heating body is attached to the front side wall of the first accommodating cavity and used for keeping the temperature inside the reagent feeding channel stable; in addition, a temperature sensor is also arranged on the inner side of the reagent feeding channel and used for monitoring the temperature change of the reagent feeding channel; a feeding limiting mechanism is arranged at the lower part of the vertical guide groove of the rear side wall in the first accommodating cavity and comprises a first limiting block, a second limiting groove, a reset spring and a hinge shaft, the first limiting block is connected with the second limiting groove through the hinge shaft, and the reset spring is arranged between the first limiting block and the second limiting groove; when the first limiting block is pressed into the second limiting groove, the reagent rack unit positioned in the first accommodating cavity falls down from the lower port of the first accommodating cavity; when the first limiting block is ejected out by the return spring, the biochemical reagent rack unit or the luminous reagent rack unit positioned in the first accommodating cavity is blocked by the first limiting block, and the biochemical reagent rack unit or the luminous reagent rack unit is kept in the first accommodating cavity.
7. The integrated biochemical-immunoassay test device according to claim 5, wherein: the reagent waste channel comprises a waste channel shell, a second accommodating cavity for accommodating a biochemical reagent rack unit or a luminous reagent rack unit which is detected is arranged in the waste channel shell, two limiting supporting bodies are arranged in the second accommodating cavity, the limiting supporting bodies are arranged at the corners of the second accommodating cavity, a waste limiting structure is arranged at the lower part of each limiting supporting body, the waste limiting structure comprises a second limiting body, the second limiting body is hinged in a second limiting groove of the limiting supporting body, and a reset spring is arranged between the second limiting body and the second limiting groove; when biochemical reagent frame unit or luminous reagent frame unit that finishes detecting enters from reagent waste passageway below, biochemical reagent frame unit or luminous reagent frame unit can extrude the second stopper, make the second stopper press to the second spacing inslot, inside biochemical reagent frame unit or luminous reagent frame unit got into reagent waste passageway completely, the second stopper popped out under reset spring effect, and biochemical reagent frame unit or luminous reagent frame unit that can restrict reagent waste passageway fall.
8. The integrated biochemical-immunoassay test device as set forth in claim 6, wherein: the left side, the right side and the rear side of the reagent rack clamping groove are respectively provided with a vertical guide block, and the vertical guide blocks are matched with the vertical guide grooves of the feeding channel shell; the reagent frame clamping groove is characterized in that a first magnetic suction part and a positioning hole are formed in the bottom of the reagent frame clamping groove, and a limiting groove is further formed in the vertical guide block on the rear side of the reagent frame clamping groove.
9. The integrated biochemical-immunoassay test device as set forth in claim 8, wherein: the reagent gripping device comprises an X-direction moving mechanism, a Z-direction moving mechanism and a moving arm;
the X-direction moving mechanism comprises an X-direction moving guide rail, an X-direction moving slide block and an X-direction power mechanism, the X-direction moving guide rail is fixed at the bottom of the shell, the X-direction moving slide block is connected with the X-direction moving guide rail in a sliding manner, and the X-direction power mechanism drives the X-direction moving slide block to move along the X-direction moving guide rail;
the Z-direction moving mechanism is fixed on the X-direction moving slide block, the Z-direction moving mechanism comprises a Z-direction moving guide rail, a Z-direction moving slide block and a Z-direction power mechanism, the Z-direction moving guide rail is fixed on the side of the X-direction moving slide block, the Z-direction moving slide block is connected with the Z-direction moving guide rail in a sliding manner, and the Z-direction power mechanism drives the Z-direction moving slide block to displace along the Z-direction moving guide rail;
the movable arm is connected to the Z-direction movable sliding block, and the tail end of the movable arm is provided with a limiting block matched with a limiting groove in the reagent rack clamping groove, a positioning screw matched with a positioning hole in the reagent rack clamping groove and a second magnetic suction part matched with the first magnetic suction part in the reagent rack clamping groove.
10. The integrated biochemical-immunoassay test device as set forth in claim 1, wherein: the sample rack device comprises a plurality of rows of sample tube fixing frames, each row of sample tube fixing frames comprises a fixed base and a test tube clamping block, and the fixed bases and the bottom of the shell are fixed by adopting a push-pull limiting structure; the test tube clamp splice is provided with a plurality of sample tube clamp grooves, and each sample tube clamp groove is used for fixing a sample tube.
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| CN213580982U (en) * | 2020-08-07 | 2021-06-29 | 中山泰辉生物科技有限公司 | Long afterglow luminous homogeneous immunoassay device |
| CN114019178A (en) * | 2021-11-08 | 2022-02-08 | 桂林优利特医疗电子有限公司 | Full-automatic immune biochemical integrated analyzer and use method thereof |
| CN114167069A (en) * | 2021-12-09 | 2022-03-11 | 桂林优利特医疗电子有限公司 | Reagent sample dish of immune biochemical integral type |
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