CN112321355A

CN112321355A - Biological organic compound bacterial fertilizer containing ginkgo element, and preparation method and application thereof

Info

Publication number: CN112321355A
Application number: CN202011220750.1A
Authority: CN
Inventors: 张翔飞; 丁超; 石启田
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2021-02-05

Abstract

The invention relates to a biological organic compound bacterial fertilizer containing ginkgo element, a preparation method and application thereof. The invention firstly uses fresh ginkgo nut testa as raw material, adopts plant extraction technology of pure physical manufacturing process to extract ginkgo element component, and uses ginkgo element, various probiotic active bacterial strain floras and other formulas to ferment so as to obtain liquid biological organic compound bacterial fertilizer (mother fertilizer) and liquid functional bacterial fertilizer of ginkgo element. And then the liquid biological organic compound bacterial manure (mother manure) of the ginkgo element, the fresh ginkgo fruits containing the testa and the active carbon, nitrogen, phosphorus, potassium and other components necessary for plant growth and soil improvement are matched with the base materials of poultry and livestock manure, kitchen residue, fish and shrimp and the like to prepare the novel solid biological organic compound bacterial manure. The invention makes the ginkgo episperm fully developed and utilized, and the obtained biological organic compound bacterial fertilizer has rich nutrient components, high fertility, no pollution, and good soil improvement and pest and disease damage inhibition effects.

Description

Biological organic compound bacterial fertilizer containing ginkgo element, and preparation method and application thereof

Technical Field

The invention belongs to the technical field of microbial aerobic fermentation, and particularly relates to a biological organic compound bacterial fertilizer containing ginkgo element, and a preparation method and application thereof.

Background

The ginkgo nut is commonly called ginkgo, and consists of two parts of fleshy testa and nutlet, the testa is smelly and is inedible, and the mass ratio of the nutlet to the testa is about 1: 3. The planting area of the ginkgo trees in China is the first in the world, and the yield of ginkgo fruits accounts for about 80% of the total amount of the world. According to statistics, about hundreds of thousands of tons of meat outer seed coat processing waste materials are discarded as waste materials every year, so that the environment is polluted, and the great resource waste is caused. Therefore, the research, development and utilization of the ginkgo biloba testa are deeply developed, not only can the environmental pollution be prevented, but also the waste can be utilized, and the waste is changed into valuable.

Previous researches show that the ginkgo biloba exocarp contains various beneficial components, contains abundant nutrient components such as amino acid and polysaccharide, and chemical components such as flavonoid, lactone, ginkgolic acid and the like, wherein the ginkgolic acid compound not only has the medicinal functions of reducing blood pressure, resisting allergy and the like, but also has strong effects of preventing and inhibiting plant diseases and insect pests. The water-soluble components contained in the ginkgo biloba sarcotesta have the inhibition rate of about 90 percent on certain pathogenic bacteria. For example, the ginkgo biloba exocarp extract has the inhibition rate of 87.9-100% on the anthracnose pathogen of apple, and the effective rate of preventing and controlling peach aphids and cabbage caterpillars can reach more than 80%. Therefore, the gingko testa has scientific value and basis for comprehensive development and utilization, and the comprehensive utilization of the gingko testa by applying biotechnology has wide development prospect. However, in the patent and literature, the utilization of the ginkgo biloba exocarp mainly focuses on the medicine and health care products, and the development and utilization of the ginkgo biloba exocarp as a biological organic compound bacterial fertilizer (plant nutrient and protective agent) are still blank in China. How to fully exert the function of the ginkgo biloba exocarp to enable the ginkgo biloba exocarp to become an organic raw material which is beneficial to improving the yield and the quality of crops and to prepare a biological organic fertilizer becomes a problem to be solved urgently.

Disclosure of Invention

In order to solve the technical problems, the invention provides a novel biological organic compound bacterial fertilizer and a preparation method thereof, and the preparation method specifically comprises the following steps:

step 1, collecting fresh ginkgo nuts, taking ginkgo episperms, and grinding the ginkgo episperms into homogenate; placing the homogenate into a container, filtering for three times, and removing sludge to obtain ginkgo biloba sarcotesta slurry; and (3) putting the slurry into a storage tank for natural precipitation, standing for 3-5 days, and collecting the precipitate to obtain the ginkgo element pure powder paste.

Step 2, weighing: 2-8 wt% of probiotics, 10-15 wt% of ginkgo element pure powder paste, 5-10 wt% of brown sugar, 30-40 wt% of raw soybean milk, 5-10 wt% of yoghourt, 1-3 wt% of fish protein and/or fish meal and the balance of water, adding the mixture into a fermentation tank, mixing and stirring the mixture until the mixture is dissolved uniformly; continuously increasing oxygen for 24 hours by using an oxygen increasing machine, wherein the power of the oxygen increasing machine is determined by a fermentation tank, and the aim is to turn liquid up and down without overflowing out of the tank body; generating yellow brown foam and rising after fermenting for 3-5 days, removing the foam by stirring or water spraying, and stopping generating the foam after fermenting for a period of time; the temperature in the fermentation tank is 25-38 ℃, the room temperature is 20-38 ℃, and the fermentation time is 28-32 days; monitoring pH value during fermentation, wherein the pH value is 3.5-4.5 after fermentation.

And 3, filtering the liquid slurry after fermentation is finished, collecting filtrate and filter residues, storing the filter residues for later use, wherein the filtrate is a mother liquor protoplasm A agent, filling the agent A into a storage tank, and storing the agent A for later use at room normal temperature.

And 4, taking the agent A obtained in the step 3 as a raw material for further fermentation, specifically: weighing the following components in parts by weight: 50-60 parts of the agent A, 1-5 parts of brown sugar (1 g of brown sugar: 50g of fermentation liquor), 0.1-0.5 part of selenium-enriched mineral powder, 3-6 parts of nitrated nitrogen, 5-8 parts of phosphorus pentoxide, 8-10 parts of potassium oxide and the balance of water in the step 3; adding the agent A and brown sugar into a fermentation tank, mixing, standing for 1-2 days, adding selenium-rich ore powder, nitrated nitrogen, phosphorus pentoxide, potassium oxide and water into the fermentation tank, stirring to dissolve, and continuously increasing oxygen for 24 hours by using an oxygen increasing machine; the temperature in the fermentation tank and the indoor temperature are 25-38 ℃, and the fermentation time is 5-7 days; when the pH value of the fermentation liquor is stabilized at 3.5-4.5 and no yellow foam appears any more, the fermentation is finished; and filtering the fermentation liquor to obtain a liquid fertilizer B agent, and filling the liquid fertilizer B agent into a storage tank for open storage. The nitrated nitrogen refers to nitrate compounds, including potassium nitrate and ammonium nitrate.

And 5, taking the agent A obtained in the step 3 as a raw material for further fermentation, specifically: weighing the following components in parts by weight: 5-10 parts of the agent A in the step 3, 1-5 parts of brown sugar, 20-30 parts of fresh ginkgo nut containing testa and/or filter residue in the step 3, 60-70 parts of livestock manure and/or kitchen residues and/or humus of animals and plants such as fish and shrimps and 3-5 parts of activated carbon; adding the agent A and brown sugar into a special tank, aerating and storing for 24-36 h to obtain a flora mother liquor of an activated strain, uniformly mixing the flora mother liquor of the activated strain and fresh ginkgo nuts containing testa and/or the filter residue, livestock manure and/or kitchen residues and/or humus of animals and plants such as fish, shrimp and the like and active carbon in the step 3, and stacking in a special fermentation tank; and (2) during the fermentation process, selecting the time when the atmospheric temperature is 20-38 ℃, turning the fermentation body to control the humidity of the fermentation body to be 40-50 wt%, fermenting for 10-15 days, finishing the fermentation when the temperature of the fermentation body is higher than and balanced at the natural temperature of 5-10 ℃ to obtain a solid ginkgo element probiotic biological organic compost base material, dehydrating the solid ginkgo element probiotic biological organic compost base material to prepare a powder or granular formulation to obtain a solid fertilizer O agent, and storing the O agent for later use. If the self-operated fermentation tank is used, the fermentation tank can be directly taken out without dehydration processing and directly used for cultivation.

Furthermore, the strains of the probiotics in the step 2 comprise photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria, rhizobia, phosphate solubilizing bacteria and bacillus.

Further, the lactic acid content of the yogurt in the step 2 is more than or equal to 98%.

Further, the selenium element content in the selenium-rich ore powder in the step 4 is 0.49 wt% -5.8 wt%.

Further, the purity of the activated carbon in the step 5 is more than or equal to 90 wt%.

Furthermore, in the step 5, animal manure and/or kitchen waste and/or animal and plant humus such as fish and shrimp are subjected to high-temperature treatment before use.

Furthermore, in the step 5, animal and plant humus such as fish and shrimp is subjected to crushing treatment before use.

The invention adopts fresh ginkgo nut which contains the testa and is peeled firstly to collect the fresh testa, and then the fresh testa is pulped, and the pulp mud and the pulp water are collected together for being used as the fermentation raw material.

The soybean milk adopted by the invention is filtered to remove residues. The yoghourt is fresh and pollution-free yoghourt.

The invention also provides an application method of the novel biological organic compound bacterial fertilizer, which comprises the following specific steps:

1) before the crops are planted, the O-dosage solid organic fertilizer produced by the invention is used as a base fertilizer for planting, generally, 150 kilograms of the O-dosage solid organic fertilizer is applied to each mu of flat-land crops for planting, then, the crops are ploughed and buried, and the quantity of the fruits and vegetables planted in the furrows is about 100 kilograms per mu of the fruits and vegetables planted in the furrows. (the paddy field can be irrigated before planting by using the A-agent bacterial liquid fertilizer, the dosage of the bacterial liquid fertilizer is 2-3 kg per mu, and each kg of the bacterial liquid fertilizer can be irrigated after being diluted by 150 kg of water)

2) After one week of crop emergence or before flowering period after seedling revival, the A-type bacterial liquid is sprayed for three to five times in the dilution ratio of 1 (bacterial liquid fertilizer) to 50-100 (water). After the flowering phase, the B-type functional bacterial liquid fertilizer is sprayed for 2-3 times in proportion.

3) Fertilizing 5 to 10 times in the whole growing period of the crops, and averagely calculating the time interval according to the growing period, wherein 0.5 kg of water and 50 kg of water are added in each mu).

The ginkgo element is a composition, and refers to an extract of ginkgo biloba containing testa, including active chemical substances such as ginkgolic acid, flavonoid and the like, and nutritional ingredients such as amino acid, polysaccharide and the like.

The invention has the beneficial technical effects that:

1. the invention uses ginkgo nuts as raw materials to prepare a novel biological organic compound bacterial fertilizer, and adopts a unique raw material formula and three original compound aerobic fermentation technologies, so that the obtained biological organic compound fertilizer containing ginkgo elements and probiotics can provide sufficient nutrition for plant growth, improve soil, play a role in preventing diseases and deinsectization, and prevent plants from being damaged by plant diseases and insect pests.

2. The method for extracting the ginkgo biloba sarcotesta is a pure physical method, has simple operation and high yield compared with the existing extraction method, and can effectively avoid potential pollution caused by a solvent method.

3. The humic substances such as fish, shrimp and the like used by the invention can provide nutrient substances such as protein and the like and promote the propagation of probiotics, the probiotics used by the invention can decompose and eliminate harmful chemical substances such as hormone, plasticizer and the like and pesticide residue, and experiments show that the removal rate of glyphosate by the probiotics used by the invention can reach 98 percent, so that the invention achieves cleanness and no pollution.

4. The invention provides a method for applying a novel biological organic compound bacterial fertilizer, which is summarized by an inventor through a large number of experiments, can obviously improve the yield and the quality of crops, can produce international standard organic food if applied according to standard amount and times (5-8 times), and can obtain the organic food which exceeds the international standard if applied for ten times or ten times according to high standard amount, thereby achieving the international inspection-free purpose.

5. The invention uses three processes of secondary to tertiary fermentation, utilizes optimized probiotic strains and a scientific formula to culture the flora mother liquor containing strong biological beneficial bacteria again, and the multi-strain probiotic flora can decompose and convert organic matter ingredients of the secondary fermentation into plant optimal nutrient amino acid and molecular form which is most easily absorbed, namely small molecular peptide, thereby not only providing various organic nutrients required by plants, but also improving soil, protecting ecological environment and enabling agricultural production to obtain sustainable virtuous cycle.

In conclusion, the novel biological organic compound bacterial fertilizer disclosed by the invention has high fertility, can also play a role in preventing plant diseases and insect pests, realizes the waste utilization of ginkgo biloba sarcotesta, and has high-quality agricultural material industrialization value.

Detailed Description

The present invention will be described in further detail with reference to the following examples and specific embodiments, but it should not be construed that the scope of the subject matter of the present invention is limited to the examples. All the technologies realized based on the above-mentioned contents of the present invention belong to the scope of the present invention, and the following embodiments are all completed by using the conventional technologies except for the specific description.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified. Specifically, the purity of the activated carbon adopted by the invention is more than or equal to 90%, the probiotic strains are purchased from agriculture technology company of Jiangsu Luoma river, the nitrified nitrogen (the purity is more than or equal to 4.5 wt%), the phosphorus pentoxide (the purity is more than or equal to 7.5 wt%) and the potassium oxide (the purity is more than or equal to 9.5 wt%) are purchased by high-quality manufacturers or companies, and the livestock manure and the kitchen residues are purchased or collected by self and are subjected to harmless treatment.

Example 1

A method for preparing biological organic compound bacterial fertilizer containing ginkgo element and plant probiotic nutrition protective agent containing ginkgo element comprises three compound fermentation technical processes of a first fermentation agent A, a second fermentation agent B and a third (solid state) fermentation agent O. The method comprises the following specific steps:

firstly, primary fermentation: a agent-ginkgo element probiotic mother liquor (liquid mother fertilizer)

1) Collecting fresh semen Ginkgo, collecting testa, and grinding the testa into slurry.

2) And (3) putting the ginkgo biloba sarcotesta slurry obtained in the step (1) into a container, repeatedly filtering for three times, and removing sludge to obtain pure ginkgo biloba sarcotesta slurry.

3) And (3) putting the slurry obtained in the step (2) into a storage tank for natural precipitation for about 3-5 days to obtain pure ginkgo element powder paste.

4) Putting probiotic strains, the ginkgo element pure powder paste obtained in the step 3 and the prepared raw materials into a fermentation tank for aerobic fermentation, wherein the mass percentage ratio is as follows:

a 8 percent of probiotics (the strains contain photosynthetic bacteria, lactobacillus, saccharomycetes, actinomycetes, filamentous bacteria, rhizobium, phosphorus solubilizing bacteria, bacillus and other strains).

b gingko extract powder 15%

c brown sugar 10%

d raw soya-bean milk 35%

e yogurt 10%

f fish protein or fish meal 3%

g natural water (balance)

5) Mixing and stirring the formula a.b.c.d.e.f.g under the step 4) until the formula is dissolved uniformly, putting an aerator and a power supply into the mixture for uninterrupted aeration for 24 hours, and starting aerobic fermentation. The power of the aerator depends on the fermentation tank, and the liquid is turned over up and down without overflowing the tank body.

6) Controlling the fermentation temperature, and keeping the temperature in the fermentation tank at 25-38 ℃.

7) The yellow brown foam is generated and rises in 3-5 days at the initial stage of fermentation, the foam is removed by stirring or water spraying at any time, and the foam is not generated after the fermentation for a period of time.

8) The fermentation container is in a sanitary and clean environment, and the indoor temperature is preferably kept between 20 ℃ and 38 ℃.

9) And (4) detecting the change of the pH value of the fermentation liquor when the fermentation time is 28-32 days, wherein the pH value is 3.5-4.5, and completing the fermentation.

10) And filtering the fermented liquid slurry again to obtain purified gingko element probiotic fermented mother liquor primary slurry.

11) And (3) filling the mother liquor raw stock purified in the step (10) into a storage tank, and storing the mother liquor raw stock indoors at normal temperature for later use.

Secondly, secondary fermentation: b agent-ginkgo element biological organic compound bacterial fertilizer (liquid functional bacterial fertilizer)

12) The raw materials are as follows according to the weight portion:

a. 8 parts of ginkgo element probiotic mother liquor (A agent protoplasm)

b. 8 portions of brown sugar

c. 0.5 part of selenium-rich mineral powder

d. 5 portions of 4.5 percent of nitrated nitrogen

e. 7.5 percent of phosphorus pentoxide and 8 parts

f. 8 portions of 9.5 percent of potassium oxide

g natural water (balance)

13) Mixing the primary pulp of the ginkgo element plant probiotic mother liquor in the items a and b with brown sugar according to the proportion of 1g to 50g, putting the mixture into a fermentation tank, and standing for 1-2 days to enhance the activity of the flora.

14) After the time of 1-2 days in the step 2), mixing the raw materials c, d, e, f and g in the step 1), putting into a fermentation tank, and stirring to dissolve.

15) Controlling the indoor temperature and the temperature in the tank to be between 25 and 38 ℃, and aerating by an aerator to perform aerobic fermentation.

16) Continuously aerating oxygen in the fermentation process, keeping the temperature constant, stabilizing the pH value of the fermentation liquid at 3.5-4.5, avoiding yellow foam, ensuring the liquid to be stable, emitting mixed fragrance of yoghourt and vinasse, and successfully fermenting.

17) The fermentation time of the agent B, namely the functional bacterial fertilizer, is shortened to 5-7 days, namely about one week, and if the fermentation is carried out continuously, the ripening is better.

18) Filtering and purifying the ginkgo element functional bacterial liquid with the fermentation liquid, and then filling the ginkgo element functional bacterial liquid into a special tank, wherein the tank opening is open, and the ginkgo element functional bacterial liquid is stored in an aerobic manner.

And thirdly, fermenting for three times: o agent-ginkgo element biological organic compound bacterial manure (solid compost)

19) The gingko element probiotic mother liquor protoplasm A prepared by the primary fermentation is mixed with the following organic matter ingredients for fermentation, and the mixture ratio by weight is as follows:

a gingko extract probiotic mother liquor (A agent protoplasm) 8 parts

b 5 parts of brown sugar

c, 30 parts of residue filtered from ginkgo biloba testa and/or agent A

70 parts of animal and plant humus such as poultry and livestock manure, smelly fish, rotten shrimp, kitchen waste and the like

e 5 parts of activated carbon

20) Firstly, the A agent prepared by the primary fermentation, namely the ginkgo element probiotic mother liquor (mother fertilizer protoplasm) is mixed with brown sugar according to the proportion of 50g to 1g, so that the probiotic dormant spores in the A agent are awakened and activated

21) Mixing the above materials at a certain ratio

22) Filling the mixed liquid into a special tank, aerating and storing for 24-36 hours to obtain the mother liquid of the activated strain flora.

23) Mixing the activated probiotic mother liquor A with the three raw materials of c, d and e under the item 19) in proportion, stirring uniformly, stacking in a special fermentation tank, and fermenting. (the fermentation tank can be arranged indoors or under a greenhouse, the opening of the fermentation tank is open and does not need to be sealed, and the fermentation agent has strong probiotic flora, so that the aerobic fermentation can be directly carried out).

24) The temperature of the fermentation tank is preferably 25-45 ℃, the time interval of the natural atmospheric temperature of 25-38 ℃ is taken as the optimal fermentation time interval, the fermentation can be continuously carried out under the condition of controlling the temperature, and the fermentation tank is not influenced by natural climate.

25) During fermentation, the water content of the fermented body is kept between 40 and 50 wt% by adopting turning and humidifying according to the water content and the temperature.

26) The fermentation period is 10-15 days, and when the temperature of the fermentation body is balanced to be slightly higher than the natural temperature by 5-10 ℃, the fermentation reaches the use standard, and the solid ginkgo element probiotic biological organic compost is obtained.

27) Processing the compost obtained in the step 26), namely airing or drying, dehydrating, and preparing into powder or granular formulation to obtain solid ginkgo element biological organic compound bacterial fertilizer (base fertilizer), and then packaging and storing for application. If the fermentation tank is used by itself, the fermentation tank can be directly taken out without dehydration processing, and the fermentation tank can be used for the cultivation.

The biological organic compound bacterial fertilizer containing the ginkgo element, namely the A agent, the B agent and the O agent, respectively comprises the following technical components and indexes:

1. the content of heavy metal Hg in a liquid A, B dosage form is less than or equal to 0.30mg/kg, the content of As is less than or equal to 1.5mg/kg, the content of Cd is less than or equal to 0.30mg/kg, the content of Pb is less than or equal to 4.0mg/kg, the effective viable count of Cr is less than or equal to 7.00mg/kg. bacterial colony is more than or equal to 6.00 hundred million/ml, the content of organic matter is more than or equal to 550g/L, the content of micronutrient elements is more than or equal to 1.8%, and the total nutrient is more than or equal to

2. The content of heavy metal Hg in a solid O dosage form is less than or equal to 0.35mg/kg, the number of effective live bacteria of a flora is more than or equal to 5.00 hundred million/ml, the content of organic matters is not less than 600g/kg, the content of micronutrients is not less than 1.50 percent, and the content of total nutrients is not less than 9.0 percent

In the indexes, the content of heavy metal is obviously lower than the national standard, and the product technical requirements are met. The effective viable count of the flora of the three bacterial fertilizers with different types exceeds the standard specified in the national agricultural microbial agent, which proves that the technical product of the invention has greatly improved and superior flora and bacterial colony count and fertilizer efficiency. In addition, other indexes of the invention all meet the national standard, and the comprehensive performance of the product reaches the national relevant requirements.

Example 2

A preparation method of biological organic compound bacterial fertilizer containing ginkgo element and ginkgo plant probiotic nutrition protective agent comprises three compound fermentation technology processes of a first fermentation agent A (liquid), a second fermentation agent B (liquid) and a third fermentation agent O (solid) and a flow.

Primary fermentation: "A agent" -gingko element probiotics mother liquor (mother fertilizer)

1) Taking fresh ginkgo biloba exocarp, peeling, collecting, and grinding the ginkgo biloba exocarp into homogenate.

2) And (3) putting the ginkgo biloba exocarp slurry obtained in the step (1) into a container, filtering, repeating the filtering for three times, and removing slag mud to obtain pure ginkgo element powder paste.

a 3% of probiotics, and the strains contain photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes, filamentous bacteria, rhizobium, phosphorus solubilizing bacteria, bacillus and other strains.

b 10% of ginkgo element pure powder paste

c brown sugar 6%

d raw soya-bean milk 33%

e 6% of yoghourt

f fish protein or fish meal 1.5%

g natural water (balance)

5) Mixing and stirring the formula a.b.c.d.e.f.g under the step 4) until the formula is dissolved uniformly, putting an aerator and a power supply into the mixture for uninterrupted aeration for 24 hours, and starting aerobic fermentation.

6) The power of the aerator depends on the fermentation tank, and the liquid is turned over up and down without overflowing the tank body.

7) Controlling the fermentation temperature, and keeping the temperature in the fermentation tank at 25-38 ℃.

8) The yellow brown foam is generated and rises in 3-5 days at the initial stage of fermentation, the foam is removed by stirring or water spraying at any time, and the foam is not generated after the fermentation for a period of time.

9) The environment of the fermentation container tank needs to be sanitary and clean, and the indoor temperature is preferably kept between 25 ℃ and 38 ℃.

10) And (4) detecting the change of the pH value of the fermentation liquor when the fermentation time is 28-32 days, wherein the pH value is 3.5-4.5, and completing the fermentation.

11) And filtering the fermented liquid slurry again to obtain purified gingko element probiotic fermented mother liquor primary slurry.

12) And (4) filling the mother liquor raw stock purified in the step (11) into a storage tank, and storing at room temperature.

Secondly, secondary fermentation: b agent-ginkgo element biological organic compound bacterial fertilizer (functional bacterial fertilizer)

13) The raw materials in parts by weight are as follows:

a 50 parts of ginkgo element probiotic mother liquor (A agent protoplasm)

b Brown sugar 6 parts

0.2 part of selenium-rich ore powder

2 parts of d-nitrated nitrogen

e phosphorus pentoxide 5 parts

8 portions of potassium f oxide

g natural water (balance)

14) Mixing the primary pulp of the ginkgo element plant probiotic mother liquor in the items a and b with brown sugar according to the proportion of 50g to 1g, putting the mixture into a fermentation tank, and standing for 1-2 days to enhance the activity of the flora.

15) After the time of step 14 is 1-2 days, the ingredients of c, d, e, f and g of the raw materials in step 13) are mixed and put into a fermentation tank, and stirred to be dissolved.

16) Controlling the indoor and tank temperature at 25-38 deg.C, and aerobic fermentation with oxygen-enriched machine.

17) Continuously aerating oxygen in the fermentation process, keeping the temperature constant, stabilizing the pH value of the fermentation liquid at 3.5-4.5, avoiding yellow foam, ensuring the liquid to be stable, emitting mixed fragrance of yoghourt and vinasse, and successfully fermenting.

18) The fermentation time of the agent B, namely the functional bacterial fertilizer, is shortened to 5-7 days, namely about one week, and if the fermentation is carried out continuously, the ripening is better.

19) Filtering and purifying the ginkgo element functional bacterial liquid with the fermentation liquid, and then filling the ginkgo element functional bacterial liquid into a special tank, wherein the tank opening is open, and the ginkgo element functional bacterial liquid is stored in an aerobic manner.

Three, O agent-ginkgo element biological organic compound bacterial fertilizer (base fertilizer)

20) Taking the ginkgo element probiotic A agent raw pulp prepared by the primary fermentation as a solid bacterial manure fermentation mother liquor, wherein the raw materials in parts by weight are as follows:

a gingko extract probiotic mother liquor (A agent protoplasm) 6 parts

b Brown sugar 3 parts

c, 25 parts of residue filtered from ginkgo biloba testa and/or agent A

60 portions of animal and plant humus such as poultry manure, kitchen residue, fish and shrimp

e 3 parts of activated carbon

21) Firstly, adding brown sugar water into the ginkgo element probiotic mother liquor protoplasm A prepared by primary fermentation for activation, wherein the ratio of the ginkgo element probiotic mother liquor protoplasm A to the brown sugar is 50g to 1g.

22) Filling the mixed liquid in the step 21) into a special tank, aerating and storing for 24-36 hours to obtain the mother liquid of the activated strain flora.

23) Mixing the mother liquor microbial inoculum obtained in the step 22) with the raw materials c, d and e in the step 20) in proportion, stirring uniformly, stacking in a special fermentation tank, and fermenting. (the fermentation tank can be arranged indoors or under a greenhouse, the opening of the fermentation tank is open and does not need to be sealed, and the fermentation agent has strong probiotic flora, so that the aerobic fermentation can be directly carried out).

24) The fermentation is carried out at the atmospheric temperature of 20-38 ℃.

26) The fermentation period is 10-15 days, and when the temperature of the fermentation body is higher than the natural temperature by 10 ℃, the fermentation reaches the use standard, so as to obtain the solid ginkgo element probiotic biological organic compost.

27) And (2) processing the compost obtained in the step (26), namely airing or drying, dehydrating, preparing into powder or granular formulation to obtain the solid ginkgo element bio-organic compound bacterial fertilizer, and then packaging and storing for later application. If the self-operated fermentation tank is used, the fermentation tank can be directly taken out without dehydration processing and directly used for cultivation.

The biological organic compound bacterial fertilizer containing the ginkgo element obtained by the technology of the invention has the technical indexes that:

the content of heavy metal Hg in a 1 liquid A, B dosage form is less than or equal to 0.30mg/kg, As is less than or equal to 1.5mg/kg, and Cd is less than or equal to

0.30mg/kg, Pb < 4.0mg/kg, Cr < 7.00mg/kg., the effective viable count of the bacteria group is more than or equal to 6.00 hundred million/ml, the organic content is more than or equal to 550g/L, the micronutrient elements are more than or equal to 1.8 percent, and the total nutrient is more than or equal to 8.5 percent

Example 3

A method for preparing biological organic compound bacterial fertilizer containing ginkgo element and gingko plant probiotic nutrition protective agent comprises three compound fermentation technical processes of a primary fermentation agent A, a secondary fermentation agent B and a tertiary fermentation agent O. The method comprises the following specific steps:

primary fermentation: a agent-ginkgo element probiotic mother liquor (mother fertilizer)

1) Collecting fresh semen Ginkgo seed coat, peeling, collecting testa, and grinding into slurry.

2) And (3) putting the ginkgo biloba sarcotesta slurry obtained in the step (1) into a container, repeatedly filtering for three times, and removing residue mud to obtain pure ginkgo biloba sarcotesta slurry.

3) Placing the slurry obtained in the step 2 into a storage tank for natural precipitation for about 3-5 days to obtain the ginkgo element pure powder paste

a 5% of probiotics, and the strains contain photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes, filamentous bacteria, rhizobium, phosphorus solubilizing bacteria, bacillus and other strains.

b ginkgo element pure powder paste 12.5%

c brown sugar 7.5%

d raw soya-bean milk 35%

e 7.5 percent of yoghourt

f fish protein or fish meal 2%

g natural water (balance)

9) The fermentation container is in a sanitary and clean environment, and the indoor temperature is preferably kept between 20 ℃ and 38 ℃.

11) And filtering the fermented liquid slurry again to obtain purified gingko element probiotic fermented mother liquor.

12) And (3) filling the mother liquor raw stock purified in the step (10) into a storage tank, and storing the mother liquor raw stock indoors at normal temperature for later use.

13) The raw materials in parts by weight are as follows:

a. 55 parts of ginkgo element probiotic mother liquor (A agent protoplasm)

b. 6.5 portions of brown sugar (1 g of brown sugar: 50g of fermentation liquor)

c. 0.3 part of selenium-rich mineral powder

d. 4.5 parts of nitrated nitrogen

e. 6.5 parts of phosphorus pentoxide

f. 9 portions of potassium oxide

Natural water (balance)

14) Mixing the primary pulp of the ginkgo element plant probiotic mother liquor of item a and item b with brown sugar water according to the proportion of 50g to 1g, putting the mixture into a fermentation tank, and standing for 1-2 days to enhance the activity of the flora.

15) After the time of the step 14) is 1-2 days, the raw materials c, d, e and f in the step 12) are mixed and put into a fermentation tank, and stirred to be dissolved.

17) Continuously aerating oxygen in the fermentation process, keeping constant temperature, ensuring that the pH value of the liquid to be fermented is stabilized at 3.5-4.5, no yellow foam appears, ensuring that the liquid is stable, emitting mixed fragrance of yoghourt and vinasse, and successfully fermenting.

19) Filtering and purifying the fermented ginkgo element functional bacterial liquid, and then filling the ginkgo element functional bacterial liquid into a special tank, wherein the tank opening is open, and the ginkgo element functional bacterial liquid is stored in an aerobic manner.

And thirdly, fermenting for three times: o agent-ginkgo element biological organic compound bacterial fertilizer (base fertilizer)

20) The ginkgo element probiotic mother liquor A agent raw stock prepared by the primary fermentation is mixed with the following organic raw materials in parts by weight for fermentation: the mixture ratio is as follows:

a gingko extract probiotic mother liquor (A agent protoplasm) 7.5 parts

b brown sugar 2.5 parts

25 parts of residue filtered from ginkgo biloba sarcotesta and agent A

65 portions of animal and plant humus such as poultry and livestock manure, fish and shrimp, kitchen residue and the like

e 4 parts of activated carbon

21) Firstly, the A agent prepared by the primary fermentation, namely the ginkgo element probiotic mother liquor (mother fertilizer protoplasm) is mixed with brown sugar according to the proportion of 50g to 1g, so that the probiotic dormant spores in the A agent are awakened and activated

22) Mixing the above materials at a certain ratio

23) Filling the mixed liquid into a special tank, aerating and storing for 24-36 hours to obtain the mother liquid of the activated strain flora.

24) Mixing the activated probiotic mother liquor A with the three raw materials of c, d and e under the item 23) in proportion, stirring uniformly, stacking in a special fermentation tank, and fermenting. (the fermentation tank can be arranged indoors or under a greenhouse, the opening of the fermentation tank is open and does not need to be sealed, and the fermentation agent has strong probiotic flora, so that the aerobic fermentation can be directly carried out).

25) The temperature of the fermentation tank is preferably 25-45 ℃, the time interval of the natural atmospheric temperature of 25-38 ℃ is taken as the optimal fermentation time interval, the fermentation can be continuously carried out under the condition of controlling the temperature, and the fermentation tank is not influenced by natural climate.

26) During fermentation, the water content of the fermented body is kept between 40 and 50 wt% by adopting turning and humidifying according to the water content and the temperature.

27) The fermentation period is 10-15 days, and when the temperature of the fermentation body is 5-10 ℃ higher than the natural temperature, the fermentation reaches the use standard, so as to obtain the solid ginkgo element probiotic biological organic compost.

28) And (2) processing the compost obtained in the step (27), namely airing or drying, dehydrating, preparing into powder or granular formulation to obtain the solid ginkgo element bio-organic compound bacterial fertilizer, and then packaging and storing for later application. If the fermentation tank is used by itself, the fermentation tank can be directly taken out without dehydration processing, and the fermentation tank can be used for the cultivation.

1. the content of heavy metal Hg in the liquid A, B dosage form is less than or equal to 0.30mg/kg, As is less than or equal to 1.5mg/kg, and Cd is less than or equal to

0.30mg/kg, Pb < 4.0mg/kg, Cr < 7.00mg/kg., the effective viable count of the bacterial colony is more than or equal to 6.00 hundred million/ml, the organic content is more than or equal to 550g/L, the micronutrient elements are more than or equal to 1.8%, and the total nutrient is more than or equal to 8.5%.

2. The number of effective viable bacteria of the solid O-type flora is not less than 5.00 hundred million/ml, the content of organic matters is not less than 600g/kg, the content of micronutrients is not less than 1.50%, and the content of total nutrients is not less than 9.0%.

Experiment one

The experimental method comprises the following steps: the biological organic compound bacterial fertilizer containing the ginkgo element obtained in the example 1 is used for carrying out planting tests, and a control group is common and common conventional planting, and grains, fruits and vegetables are planted in a field and a greenhouse. 2 mu of greenhouse, one mu of tomato and one mu of pepper are planted, and the specific fertilization scheme is shown in table 1

The experimental results are as follows: the results are shown in tables 2 and 3

TABLE 1 fertilization protocol

TABLE 2 comparison table of fruit, vegetable and grain crop total yield and quality achievement

Table 3 comparison group shows the comparison of the yield of the general bacterial manure planting and the bacterial manure using the invention

In conclusion, the biological organic compound bacterial fertilizer provided by the invention has high content of nutrient components, can inhibit crop diseases and insect pests at the same time, and realizes yield increase and income increase of agricultural planting, thereby having high-quality agricultural material industrialization value.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A preparation method of a biological organic compound bacterial fertilizer containing ginkgo element is characterized by comprising the following steps and formula:

step 1, collecting fresh ginkgo nuts, taking ginkgo episperms, and grinding the ginkgo episperms into homogenate; placing the homogenate into a container, filtering for three times, and removing sludge to obtain ginkgo biloba sarcotesta slurry; placing the slurry into a storage tank for natural precipitation, standing for 3-5 days, and collecting precipitate to obtain pure ginkgo element powder paste;

step 2, weighing:

2-8 wt% of probiotics;

10-15 wt% of ginkgo element pure powder paste;

5-10 wt% of brown sugar;

30-40 wt% of raw soybean milk;

5-10 wt% of yoghourt;

1-3 wt% of fish protein and/or fish meal;

the balance of water;

adding into a fermentation tank, mixing and stirring until the mixture is dissolved uniformly; continuously increasing oxygen for 24 hours by using an oxygen increasing machine, wherein the power of the oxygen increasing machine is determined by a fermentation tank, and the aim is to turn liquid up and down without overflowing out of the tank body; generating yellow brown foam and rising after fermenting for 3-5 days, removing the foam by stirring or water spraying, and stopping generating the foam after fermenting for a period of time; the temperature in the fermentation tank is between 25 and 38 ℃, the indoor temperature is preferably between 20 and 38 ℃, and the fermentation time is between 28 and 32 days; monitoring pH value during fermentation, wherein the pH value is 3.5-4.5 when fermentation is completed;

2. The method of claim 1, wherein the species of the probiotic bacteria include photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria, rhizobia, phospholytic bacteria, and bacillus.

3. The method according to claim 1 or 2, wherein the agent a obtained in step 3 is further fermented as a raw material, specifically: weighing the following components in parts by weight:

50-60 parts of the agent A in the step 3;

1-5 parts of brown sugar;

0.1-0.5 part of selenium-rich ore powder;

3-6 parts of nitrated nitrogen;

5-8 parts of phosphorus pentoxide;

8-10 parts of potassium oxide;

the balance of water;

adding the agent A and brown sugar into a fermentation tank, mixing, standing for 1-2 days, adding selenium-rich ore powder, nitrated nitrogen, phosphorus pentoxide, potassium oxide and water into the fermentation tank, stirring to dissolve, and continuously increasing oxygen for 24 hours by using an oxygen increasing machine; the temperature in the fermentation tank and the indoor temperature are 25-38 ℃, and the fermentation time is 5-7 days; when the pH value of the fermentation liquor is stabilized at 3.5-4.5 and no yellow foam appears any more, the fermentation is finished; and filtering the fermentation liquor to obtain a liquid fertilizer B agent, and filling the liquid fertilizer B agent into a storage tank for open storage.

4. The method according to claim 3, wherein the selenium-enriched ore powder contains 0.49 wt% to 5.8 wt% of selenium.

5. The process according to any one of claims 1 to 4, wherein the agent A obtained in step 3 is further fermented as a starting material, in particular: weighing the following components in parts by weight:

5-10 parts of the agent A in the step 3;

1-5 parts of brown sugar;

20-30 parts of fresh ginkgo nuts containing testa and the filter residue obtained in the step 3;

60-70 parts of animal and poultry manure and/or kitchen waste and/or animal and plant humus such as fish, shrimp and the like;

3-5 parts of activated carbon;

adding the agent A and brown sugar into a special tank, aerating and storing for 24-36 h to obtain a flora mother liquor of an activated strain, uniformly mixing the flora mother liquor of the activated strain, fresh ginkgo nuts containing testa and the filter residue, livestock manure and/or kitchen residues and/or humus of animals and plants such as fish, shrimps and the like and active carbon in the step 3, and stacking in a special fermentation tank; and (2) during the fermentation process, selecting the time when the atmospheric temperature is 20-38 ℃, turning the pile to control the humidity of the fermentation body to be 40-50 wt%, fermenting for 10-15 days, finishing the fermentation when the temperature of the fermentation body is higher than and balanced at the natural temperature of 5-10 ℃ to obtain a solid ginkgo element probiotic bioorganic compost base material, dehydrating the solid ginkgo element probiotic bioorganic compost base material to prepare a powder or granular formulation to obtain a solid bacterial manure O agent, and storing the O agent for later use.

6. The method according to claim 5, wherein the animal manure and/or kitchen waste and/or humus of animals and plants such as fish and shrimp is sterilized at high temperature before use; before use, the humus of animals and plants such as fish, shrimp and the like is crushed; the purity of the active carbon is more than or equal to 90 wt%.

7. A bio-organic composite bacterial fertilizer, which is characterized by being prepared by the preparation method of claim 1 or 2.

8. A biological organic compound bacterial fertilizer, which is characterized by being prepared by the preparation method of claim 3 or 4.

9. A bio-organic compound bacterial fertilizer, which is characterized by being prepared by the preparation method of claim 5 or 6.

10. A fertilizer application method is characterized in that the biological organic compound bacterial fertilizer of claim 7 is used as a foliar fertilizer, the biological organic compound bacterial fertilizer of claim 8 is used as a water flush fertilizer, and the biological organic compound bacterial fertilizer of claim 9 is used as a base fertilizer.